Nucleoporin GLE1

Overview
NameNucleoporin GLE1
Smed IDSMED30002248
Length (bp)1406
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Nucleoporin GLE1 (SMED30002248) t-SNE clustered cells

Violin plots show distribution of expression levels for Nucleoporin GLE1 (SMED30002248) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Nucleoporin GLE1 (SMED30002248) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Nucleoporin GLE1 (SMED30002248) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002248

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30002248h1SMcG0001974 dd_Smed_v4_3943_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30002248h1SMcG0001974 dd_Smed_v4_3943_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30002248h1SMcG0001974 dd_Smed_v4_3943_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30002248h1SMcG0001974 dd_Smed_v4_3943_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of Nucleoporin GLE1 vs. TrEMBL
Match: U6IVS5 (Nucleoporin GLE1 OS=Echinococcus granulosus OX=6210 GN=EGR_05979 PE=4 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 2.618e-21
Identity = 71/257 (27.63%), Postives = 122/257 (47.47%), Query Frame = 3 
Query:  642 IRKFVNNICSQITSDNPNHALGRLQDLINLLNKADVPLVGTEGFINLNQHPRANEIFWEFLRNAVIVQAQTQFIVNIKTAIVFSSLLAAVLGQFPTKLAAMLQALEAKFSHLLKXXXXXXXXXXXXXXQLN-----SLIGLARLVAGISVAHPPPGFPVHPE-LGPAFLWALVARICNEQSVSPFTAQACIGLLEVGGHSLWRAYGHQFEKLLTALLDKFSVSTNLTVRSLNLLITTSLDNRSFEQLGY--LTNEFW 1388
            ++  + + CSQIT  +P+H L RL  L+     A V   G    +            W  L NA + QA+ QF  ++ + + F+++LA +L ++P  +   L  + +    L   +D         + +L+     S  G++RL A + +A PPPGF   PE L P  LW  +A I   +     T++   GL+EVGG+ L + Y  Q  +L  +L+D    S +++  +L   +   LD  +  + G+  L ++FW
Sbjct:  226 VKMIIGSACSQITGSDPDHCLNRLNYLLAFFEGAPVRPPGAAAEVFRLPQDMGTAFAWHCLLNAALAQAERQFAFSLHSVLPFAAVLATLLARYPEYIPNFLARMASACPVLTSYVDDSATIFASLDVELDEGVLQSWRGISRLFAAVLLARPPPGFTHRPEHLNPKLLWITIAGIARHEGKLDITSEVLHGLIEVGGYVLLQLYQRQARRLFKSLIDAVGDSQSVSQLALITCLEQLLDPTTATENGHGVLLDDFW 482
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BLAST of Nucleoporin GLE1 vs. TrEMBL
Match: A0A068Y683 (Nucleoporin GLE1 OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000755400 PE=4 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 4.722e-21
Identity = 70/257 (27.24%), Postives = 121/257 (47.08%), Query Frame = 3 
Query:  642 IRKFVNNICSQITSDNPNHALGRLQDLINLLNKADVPLVGTEGFINLNQHPRANEIFWEFLRNAVIVQAQTQFIVNIKTAIVFSSLLAAVLGQFPTKLAAMLQALEAKFSHLLKXXXXXXXXXXXXXXQLNSLI-----GLARLVAGISVAHPPPGFPVHPE-LGPAFLWALVARICNEQSVSPFTAQACIGLLEVGGHSLWRAYGHQFEKLLTALLDKFSVSTNLTVRSLNLLITTSLDNRSFEQLGY--LTNEFW 1388
            ++  + + CSQIT  +P H L RL  L+     A V   G    +            W  L NA + QA+ QF  ++ + + F+++LA +L ++P  +   L  + +    L   +D         + +L+  +     G++RL A + +A PPPGF   PE L P  LW  +A I   +     T++   GL+EVGG+ L + Y  Q  +L  +L+D    S +++  +L   +   LD  +  + G+  L ++FW
Sbjct:  226 VKMIIGSACSQITGSDPEHCLNRLNYLLAFFEGAPVRPPGAAAEVFRLPQDMGMAFAWHCLLNAALAQAERQFAFSLHSVLPFAAVLATLLARYPEYIPNFLARMASACPVLTSYVDDSATTFASLDVELDEGVLQCWRGISRLFAAVLLARPPPGFTQRPEHLNPKLLWITIAGIARHEGKLDITSEVLHGLIEVGGYVLLQLYQRQARRLFKSLIDAVGDSQSVSQLALITCLEQLLDPTTATENGHGVLLDDFW 482
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BLAST of Nucleoporin GLE1 vs. TrEMBL
Match: G4VJF6 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_087340 PE=4 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 7.252e-20
Identity = 64/218 (29.36%), Postives = 110/218 (50.46%), Query Frame = 3 
Query:  642 IRKFVNNICSQITSDNPNHALGRLQDLINLLNKADVPLVGTEGFI--NLNQHPRANEIFWEFLRNAVIVQAQTQFIVNIKTAIVFSSLLAAVLGQFPTKL----AAMLQALEAKFSHLLKXXXXXXXXXXXXXXQLNSLIGLARLVAGISVAHPPP--GFPVHPELGPAFLWALVARICNEQSVSPFTAQACIGLLEVGGHSLWRAYGHQFEKLLTAL 1271
            I++ +   CSQI+S +P H +GRL+ L+N+  +  +     +  +  + N +   +   W+ L ++ I QA+ QF  NI   +V++SLL+ +   FP KL      +  A  A             ++     + L++  G+ARL   + +AHPPP  G   H  L P+ LW ++A I N++ +   TA+   G+LE+GG      YG Q ++LL  +
Sbjct:  255 IKQDITCACSQISSHDPPHCIGRLECLLNIFRRTTITDCSEQTKLGKSRNTNQLVDSFAWQCLYSSSISQAEKQFAYNIDITLVYASLLSGIFALFPDKLFEFFGRIFLACPALGLFSDPSGLNLIEEMFSTTDILSNWRGVARLFTALLLAHPPPHLGVSRHKLLNPSLLWRVIAGIVNQEFIPCATAEVLHGILEIGGSVFANLYGKQAKRLLNTI 472
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BLAST of Nucleoporin GLE1 vs. TrEMBL
Match: A0A0R3VTX9 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS730 PE=4 SV=2)

HSP 1 Score: 102.834 bits (255), Expect = 7.664e-20
Identity = 73/256 (28.52%), Postives = 119/256 (46.48%), Query Frame = 3 
Query:  645 RKFVNNICSQITSDNPNHALGRLQDLINLLNKADVPLVGTEGFINLNQHPRANEIFWEFLRNAVIVQAQTQFIVNIKTAIVFSSLLAAVLGQF----PTKLAAMLQALEAKFSHLLKXXXXXXXXXXXXXXQ-LNSLIGLARLVAGISVAHPPPGFPVHPE-LGPAFLWALVARICNEQSVSPFTAQACIGLLEVGGHSLWRAYGHQFEKLLTALLDKFSVSTNLTVRSLNLLITTSLDNRSFEQLGY--LTNEFW 1388
            +  + + CSQIT  +P H L RL  L+       V   G    +            W  L NA + QA+ QF  ++++ + F+++LA +L ++    P  LA M  A     S++     +    + + ++  L S  G++RL A + +A PPPGF   PE L P  LW  +A I         T++   GL+EVGG+ L + Y  Q  +L  +L+D    S  ++  +L   +   LD  +    GY  L ++FW
Sbjct:  227 KMIIGSACSQITGSDPEHCLNRLNYLLAFFEGEPVRPPGAAADVFRLPESMGTVFAWHCLLNAALAQAERQFAFSLQSVLPFAAVLATLLARYSEYTPNFLARMASACPVLTSYVDDSTTIFASLDAELDDGVLQSWRGISRLFAAVLLARPPPGFTQRPEHLNPKLLWITIAGIAKHTDKFSVTSEVLHGLIEVGGNVLLKLYRRQARRLFKSLIDAIGDSPPVSQLALTTCLEQLLDPATAVDNGYGVLLDDFW 482
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BLAST of Nucleoporin GLE1 vs. TrEMBL
Match: A0A5K4EHM1 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.117e-19
Identity = 69/235 (29.36%), Postives = 116/235 (49.36%), Query Frame = 3 
Query:  600 IEYESDLNVHYKNNIRKFVNNI---CSQITSDNPNHALGRLQDLINLLNKADVPLVGTEGFI--NLNQHPRANEIFWEFLRNAVIVQAQTQFIVNIKTAIVFSSLLAAVLGQFPTKL----AAMLQALEAKFSHLLKXXXXXXXXXXXXXXQLNSLIGLARLVAGISVAHPPP--GFPVHPELGPAFLWALVARICNEQSVSPFTAQACIGLLEVGGHSLWRAYGHQFEKLLTAL 1271
            I Y S  +  +K +  K   +I   CSQI+S +P H +GRL+ L+N+  +  +     +  +  + N +   +   W+ L ++ I QA+ QF  NI   +V++SLL+ +   FP KL      +  A  A             ++     + L++  G+ARL   + +AHPPP  G   H  L P+ LW ++A I N++ +   TA+   G+LE+GG      YG Q ++LL  +
Sbjct:  155 ILYPSLWDSKFKMDALKIKQDITCACSQISSHDPPHCIGRLECLLNIFRRTTITDCSEQTKLGKSRNTNQLVDSFAWQCLYSSSISQAEKQFAYNIDITLVYASLLSGIFALFPDKLFEFFGRIFLACPALGLFSDPSGLNLIEEMFSTTDILSNWRGVARLFTALLLAHPPPHLGVSRHKLLNPSLLWRVIAGIVNQEFIPCATAEVLHGILEIGGSVFANLYGKQAKRLLNTI 389
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BLAST of Nucleoporin GLE1 vs. Ensembl Sea Lamprey
Match: gle1 (GLE1 RNA export mediator [Source:NCBI gene;Acc:445408])

HSP 1 Score: 48.1358 bits (113), Expect = 3.091e-6
Identity = 31/88 (35.23%), Postives = 49/88 (55.68%), Query Frame = 3 
Query: 1029 LNSLIGLARLVAGISVAHPPPGFPV---HPELGPAFLWALVARICNEQSVSPFTAQACIGLLEVGGHSLWRAYGHQFEKLLTALLDKF 1283
            LN + G+ RL A I +A P P   +   HP  G  + W  +A++ N + VS  +A      LEV G+++ RAY  QF KLL  +++++
Sbjct:  414 LNRMSGMMRLYAAI-IATPVPSPSMNKSHPH-GLQYGWRWIAQMLNLEPVSDISATMLYDFLEVAGNAMERAYCSQFWKLLFFIIEEY 499
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BLAST of Nucleoporin GLE1 vs. Ensembl Nematostella
Match: EDO28097 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T7F4])

HSP 1 Score: 49.2914 bits (116), Expect = 9.341e-7
Identity = 31/85 (36.47%), Postives = 41/85 (48.24%), Query Frame = 3 
Query: 1029 LNSLIGLARLVAGISVAHPPPGFPVHPELGPAFLWALVARICNEQSVSPFTAQACIGLLEVGGHSLWRAYGHQFEKLLTALLDKF 1283
            L  + G  RL A I  + PP G      LG +  W  +AR+ N    + +TA A    LEV GH L + Y  QF KL+  L  +F
Sbjct:  101 LKRMTGTVRLYAAIMSSPPPKGTAQPHPLGISHGWTWLARVLNISPRADYTATAVYEFLEVAGHVLVKLYRGQFWKLMQILCQEF 185
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BLAST of Nucleoporin GLE1 vs. Planmine SMEST
Match: SMESG000003757.1 (SMESG000003757.1)

HSP 1 Score: 862.062 bits (2226), Expect = 0.000e+0
Identity = 436/438 (99.54%), Postives = 437/438 (99.77%), Query Frame = 3 
Query:   87 MNEIKDNKKCDPDELIYRILSTIETFNSRKIKLQKYKRIVTPLKVVNKPNIDYTIDTSKRNANEEFLTFQKSVEIFNHLYIKNGEYISKYFECELNSLYDCVKIILNYFNEISIEGSNVTMVNFEKLSLIQLAIEFQVKGLELLLVDSKNKYKMEIEKTLAEKQMQQEIKRIEYESDLNVHYKNNIRKFVNNICSQITSDNPNHALGRLQDLINLLNKADVPLVGTEGFINLNQHPRANEIFWEFLRNAVIVQAQTQFIVNIKTAIVFSSLLAAVLGQFPTKLAAMLQALEAKFSHLLKXXXXXXXXXXXXXXQLNSLIGLARLVAGISVAHPPPGFPVHPELGPAFLWALVARICNEQSVSPFTAQACIGLLEVGGHSLWRAYGHQFEKLLTALLDKFSVSTNLTVRSLNLLITTSLDNRSFEQLGYLTNEFWTGTN 1400
            MNEIKDNKKCDPDELIYRILSTIETFNSRKIKLQKYKRIVTPLKVVNKPNIDYTIDTSKRNANEEFLTFQKSVEIFNHLYIKNGEYISKYFECELNSLYDCVKIILNYFNEISIEGSNVTMVNFEKLSLIQLAIEFQVKGLELLLVDSKNKYKMEIEKTLAEKQMQQEIKRIEYESDLNVHYKNNIRKFVNNICSQITSDNP HALGRLQDLINLLNKADVPLVGTEGFINLNQHPRANEIFWEFLRNAVIVQAQTQFIVNIKTAIVFSSLLAAVLGQFPTKLAAMLQALEAKFSHLLKVIDVDDDDEIDEEEQLNSLIGLARLVAGIS+AHPPPGFPVHPELGPAFLWALVARICNEQSVSPFTAQACIGLLEVGGHSLWRAYGHQFEKLLTALLDKFSVSTNLTVRSLNLLITTSLDNRSFEQLGYLTNEFWTGTN
Sbjct:    1 MNEIKDNKKCDPDELIYRILSTIETFNSRKIKLQKYKRIVTPLKVVNKPNIDYTIDTSKRNANEEFLTFQKSVEIFNHLYIKNGEYISKYFECELNSLYDCVKIILNYFNEISIEGSNVTMVNFEKLSLIQLAIEFQVKGLELLLVDSKNKYKMEIEKTLAEKQMQQEIKRIEYESDLNVHYKNNIRKFVNNICSQITSDNPTHALGRLQDLINLLNKADVPLVGTEGFINLNQHPRANEIFWEFLRNAVIVQAQTQFIVNIKTAIVFSSLLAAVLGQFPTKLAAMLQALEAKFSHLLKVIDVDDDDEIDEEEQLNSLIGLARLVAGISIAHPPPGFPVHPELGPAFLWALVARICNEQSVSPFTAQACIGLLEVGGHSLWRAYGHQFEKLLTALLDKFSVSTNLTVRSLNLLITTSLDNRSFEQLGYLTNEFWTGTN 438
The following BLAST results are available for this feature:
BLAST of Nucleoporin GLE1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nucleoporin GLE1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nucleoporin GLE1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nucleoporin GLE1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nucleoporin GLE1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nucleoporin GLE1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nucleoporin GLE1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nucleoporin GLE1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
U6IVS52.618e-2127.63Nucleoporin GLE1 OS=Echinococcus granulosus OX=621... [more]
A0A068Y6834.722e-2127.24Nucleoporin GLE1 OS=Echinococcus multilocularis OX... [more]
G4VJF67.252e-2029.36Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A0R3VTX97.664e-2028.52Uncharacterized protein OS=Taenia asiatica OX=6051... [more]
A0A5K4EHM11.117e-1929.36Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
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BLAST of Nucleoporin GLE1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nucleoporin GLE1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gle13.091e-635.23GLE1 RNA export mediator [Source:NCBI gene;Acc:445... [more]
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BLAST of Nucleoporin GLE1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nucleoporin GLE1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO280979.341e-736.47Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Nucleoporin GLE1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nucleoporin GLE1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000003757.10.000e+099.54SMESG000003757.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002248 ID=SMED30002248|Name=Nucleoporin GLE1|organism=Schmidtea mediterranea sexual|type=transcript|length=1406bp
GAAAAATTTATTTTTTATTGAATATTGAAATTTAGTTATATTTTCATTAT
TTTAAGTATAATAAAATATATTTTTAAATCAGTTTTATGAATGAAATAAA
AGATAATAAAAAATGTGATCCAGATGAACTCATCTATCGTATATTATCTA
CAATAGAAACATTTAATTCCAGAAAAATAAAATTACAAAAATATAAAAGA
ATTGTTACACCATTGAAAGTTGTGAATAAACCTAATATTGATTATACTAT
AGATACAAGTAAACGCAATGCAAATGAAGAATTTCTGACTTTTCAGAAAT
CTGTAGAGATTTTTAATCATTTATATATTAAAAATGGAGAATATATTTCA
AAATACTTTGAATGTGAGTTAAATTCATTATATGATTGTGTGAAAATAAT
CTTAAATTATTTCAACGAAATATCGATTGAAGGATCAAACGTCACAATGG
TAAATTTTGAAAAATTGTCACTAATACAATTAGCCATCGAGTTTCAAGTA
AAAGGATTGGAGCTACTGTTGGTTGATTCAAAAAATAAATATAAAATGGA
AATTGAGAAAACCCTCGCGGAAAAACAAATGCAACAAGAAATTAAGAGAA
TCGAATATGAATCAGATCTTAATGTACATTACAAAAATAATATACGAAAA
TTTGTCAATAATATTTGTAGCCAAATTACCAGCGATAATCCCAATCATGC
GCTTGGACGGTTGCAGGATTTGATTAACTTGTTAAATAAAGCCGATGTGC
CATTAGTGGGAACTGAAGGATTCATTAACCTCAATCAACATCCAAGAGCC
AATGAAATCTTCTGGGAATTTCTCCGCAATGCAGTGATTGTGCAAGCTCA
AACCCAATTCATTGTCAACATAAAAACGGCAATTGTCTTTTCCAGTTTAC
TAGCCGCAGTTCTCGGCCAATTTCCAACTAAACTGGCGGCAATGTTGCAG
GCGCTCGAAGCGAAATTCTCTCATTTATTGAAGGTGATTGATGTTGACGA
TGACGATGAGATTGATGAAGAGGAACAATTGAATAGTTTGATTGGCTTAG
CCCGACTTGTTGCCGGAATCAGTGTAGCGCATCCACCGCCGGGTTTCCCG
GTTCATCCTGAGCTCGGGCCGGCATTTCTATGGGCGCTGGTAGCGCGCAT
TTGCAATGAGCAGTCCGTTTCACCATTCACTGCACAGGCGTGCATCGGAC
TGCTGGAGGTCGGAGGCCATTCGTTGTGGCGCGCATACGGACACCAATTC
GAGAAACTATTGACTGCGTTGCTGGACAAGTTCAGCGTGTCCACTAATTT
GACTGTGAGATCATTGAACCTGCTTATCACGACTTCACTTGATAACAGAT
CATTCGAGCAACTCGGATATTTAACTAATGAGTTTTGGACGGGCACGAAT
TGATGC
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protein sequence of SMED30002248-orf-1

>SMED30002248-orf-1 ID=SMED30002248-orf-1|Name=SMED30002248-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=101bp
HQFVPVQNSLVKYPSCSNDLLSSEVVISRFNDLTVKLVDTLNLSSNAVNS
FSNWCPYARHNEWPPTSSSPMHACAVNGETDCSLQMRATSAHRNAGPSSG
*
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protein sequence of SMED30002248-orf-2

>SMED30002248-orf-2 ID=SMED30002248-orf-2|Name=SMED30002248-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=439bp
MNEIKDNKKCDPDELIYRILSTIETFNSRKIKLQKYKRIVTPLKVVNKPN
IDYTIDTSKRNANEEFLTFQKSVEIFNHLYIKNGEYISKYFECELNSLYD
CVKIILNYFNEISIEGSNVTMVNFEKLSLIQLAIEFQVKGLELLLVDSKN
KYKMEIEKTLAEKQMQQEIKRIEYESDLNVHYKNNIRKFVNNICSQITSD
NPNHALGRLQDLINLLNKADVPLVGTEGFINLNQHPRANEIFWEFLRNAV
IVQAQTQFIVNIKTAIVFSSLLAAVLGQFPTKLAAMLQALEAKFSHLLKV
IDVDDDDEIDEEEQLNSLIGLARLVAGISVAHPPPGFPVHPELGPAFLWA
LVARICNEQSVSPFTAQACIGLLEVGGHSLWRAYGHQFEKLLTALLDKFS
VSTNLTVRSLNLLITTSLDNRSFEQLGYLTNEFWTGTN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR038506GLE1-like_sf
IPR012476GLE1
Vocabulary: cellular component
TermDefinition
GO:0005643nuclear pore
Vocabulary: biological process
TermDefinition
GO:0016973poly(A)+ mRNA export from nucleus
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012476GLE1-likePFAMPF07817GLE1coord: 164..286
e-value: 2.8E-8
score: 33.3
coord: 309..395
e-value: 4.1E-10
score: 39.3
IPR012476GLE1-likePANTHERPTHR12960GLE-1-RELATEDcoord: 163..281
coord: 311..399
IPR038506GLE1-like superfamilyGENE3DG3DSA:1.25.40.510coord: 298..425
e-value: 1.5E-11
score: 45.7