Nucleobindin 2

Overview


Name	Nucleobindin 2
Smed ID	SMED30002242
Length (bp)	1242

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Nucleobindin 2 (SMED30002242) t-SNE clustered cells

Violin plots show distribution of expression levels for Nucleobindin 2 (SMED30002242) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Nucleobindin 2 (SMED30002242) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Nucleobindin 2 (SMED30002242) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30002242

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 12

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
pigment cell	SMED30002242	SMESG000016720.1	SmedASXL_019713	SmedAsxl_ww_GCZZ01	PMID:29158443 He et al., 2017
X1 cell	SMED30002242	SMESG000016720.1	SmedASXL_019713	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
X2 cell	SMED30002242	SMESG000016720.1	SmedASXL_019713	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
epidermis	SMED30002242	SMESG000016720.1	dd_Smed_v4_6912_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30002242	SMESG000016720.1	dd_Smed_v4_6912_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30002242	SMESG000016720.1	dd_Smed_v4_6912_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30002242	SMESG000016720.1	Contig38455	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30002242	SMESG000016720.1	Contig38455	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
gut	SMED30002242	SMESG000016720.1	SMED30002242	smed_20140614	PMID:26457503 Tu et al., 2015
parenchyma	SMED30002242	SMESG000016720.1	SMED30002242	smed_20140614	PMID:26457503 Tu et al., 2015
Category 2 cell	SMED30002242	SMESG000016720.1	dd_Smed_v6_6912_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
Category 2 cell	SMED30002242	SMESG000016720.1	dd_Smed_v4_6912_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of Nucleobindin 2 vs. Ensembl Human
Match: NUCB1 (nucleobindin 1 [Source:HGNC Symbol;Acc:HGNC:8043])

HSP 1 Score: 123.25 bits (308), Expect = 2.309e-30
Identity = 86/247 (34.82%), Postives = 139/247 (56.28%), Query Frame = 1

Query:  337 EIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKS--KRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIE-WKTSE 1065
            E+K +E++RL        RML  +   ++ + +   D+L   ++  HL       F   D+E L+     DL   D    E+++RYE  K+   R+ LE   +  +K   ++LEE    ++ +R+H ++N PGS+ Q KE+W+ +D LD + FN KTFF LHD+N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD ++D L+TL+E +  T RK+F    E W+T E
Sbjct:  102 ELKRQEVSRL--------RMLLKAKMDAEQDPNVQVDHLNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEE---QQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWETVE 337

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Query:  337 EIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKS--KRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIE-WKTSE 1065
            E+K +E++RL        RML  +   ++ + +   D+L   ++  HL       F   D+E L+     DL   D    E+++RYE  K+   R+ LE   +  +K   ++LEE    ++ +R+H ++N PGS+ Q KE+W+ +D LD + FN KTFF LHD+N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD ++D L+TL+E +  T RK+F    E W+T E
Sbjct:  102 ELKRQEVSRL--------RMLLKAKMDAEQDPNVQVDHLNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEE---QQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWETVE 337

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Human
Match: NUCB1 (nucleobindin 1 [Source:HGNC Symbol;Acc:HGNC:8043])

HSP 1 Score: 114.775 bits (286), Expect = 2.035e-28
Identity = 71/189 (37.57%), Postives = 111/189 (58.73%), Query Frame = 1

Query:  472 HLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKS--KRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKD 1032
            HL       F   D+E L+     DL   D    E+++RYE  K+   R+ LE   +  +K   ++LEE    ++ +R+H ++N PGS+ Q KE+W+ +D LD + FN KTFF LHD+N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD ++D L+TL+E +  T RK+
Sbjct:  110 HLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEE---QQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKE 295

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Human
Match: NUCB2 (nucleobindin 2 [Source:HGNC Symbol;Acc:HGNC:8044])

HSP 1 Score: 112.079 bits (279), Expect = 1.168e-26
Identity = 106/345 (30.72%), Postives = 173/345 (50.14%), Query Frame = 1

Query:   82 MCYCTFILYFAVLSTFCTSSSSEQIEKFIDELNQKE-----------------YSEYMRNVMQELLKDKNIQGKIFNQNSTSNKD--FMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKT 1059
            M + T +L +  L   C  ++ E +   ID+   +                  Y EY++ V+  L  DK+ + K+   +    K       LDL S   R+KLDE+K +E+ RL        RML  + KL   +   M       +  HL+     KF   D++ L+     DL   D  R E++++YE  K+   R+E  K  +  ++ +   + ++ +K +  H ++N PGS+DQ KE+W+  D LD + F+ KTFF LHD+N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M +VD +KD L+TL+E +  T +K+F     W+T
Sbjct:    1 MRWRTILLQYCFLLITCLLTALEAVPIDIDKTKVQNIHPVESAKIEPPDTGLYYDEYLKQVIDVLETDKHFREKLQKADIEEIKSGRLSKELDLVSHHVRTKLDELKRQEVGRL--------RMLIKA-KLDSLQDIGMDHQALLKQFDHLNHLNPDKFESTDLDMLIKAATSDLEHYDKTRHEEFKKYEMMKEHE-RREYLKTLNEEKRKEEESKFEEMKKKHENHPKVNHPGSKDQLKEVWEETDGLDPNDFDPKTFFKLHDVNSDGFLDEQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMNEVDTNKDRLVTLEEFLKATEKKEFLEPDSWET 335

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Human
Match: NUCB2 (nucleobindin 2 [Source:HGNC Symbol;Acc:HGNC:8044])

HSP 1 Score: 112.079 bits (279), Expect = 1.448e-26
Identity = 106/345 (30.72%), Postives = 173/345 (50.14%), Query Frame = 1

Query:   82 MCYCTFILYFAVLSTFCTSSSSEQIEKFIDELNQKE-----------------YSEYMRNVMQELLKDKNIQGKIFNQNSTSNKD--FMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKT 1059
            M + T +L +  L   C  ++ E +   ID+   +                  Y EY++ V+  L  DK+ + K+   +    K       LDL S   R+KLDE+K +E+ RL        RML  + KL   +   M       +  HL+     KF   D++ L+     DL   D  R E++++YE  K+   R+E  K  +  ++ +   + ++ +K +  H ++N PGS+DQ KE+W+  D LD + F+ KTFF LHD+N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M +VD +KD L+TL+E +  T +K+F     W+T
Sbjct:    1 MRWRTILLQYCFLLITCLLTALEAVPIDIDKTKVQNIHPVESAKIEPPDTGLYYDEYLKQVIDVLETDKHFREKLQKADIEEIKSGRLSKELDLVSHHVRTKLDELKRQEVGRL--------RMLIKA-KLDSLQDIGMDHQALLKQFDHLNHLNPDKFESTDLDMLIKAATSDLEHYDKTRHEEFKKYEMMKEHE-RREYLKTLNEEKRKEEESKFEEMKKKHENHPKVNHPGSKDQLKEVWEETDGLDPNDFDPKTFFKLHDVNSDGFLDEQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMNEVDTNKDRLVTLEEFLKATEKKEFLEPDSWET 335

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BLAST of Nucleobindin 2 vs. Ensembl Celegans
Match: nucb-1 (NUCleoBindin homolog [Source:UniProtKB/TrEMBL;Acc:Q8MQ51])

HSP 1 Score: 105.916 bits (263), Expect = 9.605e-25
Identity = 62/180 (34.44%), Postives = 116/180 (64.44%), Query Frame = 1

Query:  472 HLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELI 1011
            HL   E  KF K+D+ KL+ +   D+ ++D  R++ +++YE +K      +L +   P ++ K   E ++S K +  H+++  PGS+DQ +E+W+  D+L+   ++ KTFF LHDLN DG W+  E+E++ Q ++EK+Y + N   D +E+  E+ +MR+H+M+++D ++D +I++QE +
Sbjct:  153 HLDVQELEKFHKEDLRKLIQKTVADMNVMDEQRKDDFKQYEMKKQAEEDHKLAQMT-PEEREKAKSEHEESLKRHNDHEKLKHPGSKDQLQEVWEESDHLEKDQYDPKTFFALHDLNGDGFWNDFELESLFQLELEKMYNETNPDDDMKERAEEMYRMREHVMKQIDVNQDRMISMQEFL 331

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Celegans
Match: nucb-1 (NUCleoBindin homolog [Source:UniProtKB/TrEMBL;Acc:Q8MQ51])

HSP 1 Score: 105.531 bits (262), Expect = 1.454e-24
Identity = 62/180 (34.44%), Postives = 116/180 (64.44%), Query Frame = 1

Query:  472 HLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELI 1011
            HL   E  KF K+D+ KL+ +   D+ ++D  R++ +++YE +K      +L +   P ++ K   E ++S K +  H+++  PGS+DQ +E+W+  D+L+   ++ KTFF LHDLN DG W+  E+E++ Q ++EK+Y + N   D +E+  E+ +MR+H+M+++D ++D +I++QE +
Sbjct:  153 HLDVQELEKFHKEDLRKLIQKTVADMNVMDEQRKDDFKQYEMKKQAEEDHKLAQMT-PEEREKAKSEHEESLKRHNDHEKLKHPGSKDQLQEVWEESDHLEKDQYDPKTFFALHDLNGDGFWNDFELESLFQLELEKMYNETNPDDDMKERAEEMYRMREHVMKQIDVNQDRMISMQEFL 331

[Close]

Query:  472 HLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELI 1011
            HL   E  KF K+D+ KL+ +   D+ ++D  R++ +++YE +K      +L +   P ++ K   E ++S K +  H+++  PGS+DQ +E+W+  D+L+   ++ KTFF LHDLN DG W+  E+E++ Q ++EK+Y + N   D +E+  E+ +MR+H+M+++D ++D +I++QE +
Sbjct:  153 HLDVQELEKFHKEDLRKLIQKTVADMNVMDEQRKDDFKQYEMKKQAEEDHKLAQMT-PEEREKAKSEHEESLKRHNDHEKLKHPGSKDQLQEVWEESDHLEKDQYDPKTFFALHDLNGDGFWNDFELESLFQLELEKMYNETNPDDDMKERAEEMYRMREHVMKQIDVNQDRMISMQEFL 331

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Fly
Match: NUCB1 (gene:FBgn0052190 transcript:FBtr0075171)

HSP 1 Score: 94.7449 bits (234), Expect = 1.010e-20
Identity = 85/293 (29.01%), Postives = 161/293 (54.95%), Query Frame = 1

Query:  187 EYSEYMRNVMQELLKDKNIQGKI--FNQNSTSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKT 1059
            EY  Y+R V++ L  D   + K+    +    +      LD  +   R+KLDEIK RE+ RL  L  Q       + +LS N+ D     ++     HL    +  F  +D+ KL+ +   DL   D  R  +++ YE QK++  R+  +K  D   + K   E+K+  + ++ H++++ PG++ Q +++W+  D++D + F+ KTFF++HD++ +G WD  E++A+   +++K+Y+      D  E+  E+ +MR+H  ++ D + D LI++ E +V T +++F+ + EW+T
Sbjct:   50 EYERYLREVVEALEADPEFRKKLDKAPEADIRSGKIAQELDYVNHHVRTKLDEIKRREVERLRELANQ-------AYELS-NDIDRKHLKVSQ----HLDHDNEHTFEIEDLRKLIQKTSDDLAEADRKRRGEFKEYEMQKEFE-REAQKKEMDEESRKKFETELKEKEEKHKDHEKLHHPGNKAQLEDVWEKQDHMDKNDFDPKTFFSIHDVDSNGYWDEAEVKALFVKELDKVYQSDLPEDDMRERAEEMERMREHYFQETDMNHDGLISIDEFMVQTNKEEFQKDPEWET 329

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BLAST of Nucleobindin 2 vs. Ensembl Zebrafish
Match: nucb2b (nucleobindin 2b [Source:ZFIN;Acc:ZDB-GENE-030131-4775])

HSP 1 Score: 127.102 bits (318), Expect = 3.511e-32
Identity = 109/335 (32.54%), Postives = 177/335 (52.84%), Query Frame = 1

Query:   88 YCTFILYF-AVLSTFCTSSSSEQIEKFIDELNQKE-------YSEYMRNVMQELLKDKNIQGKIFNQNSTSNKD--FMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKT 1059
            +C  IL   A L     +    ++    +     E       Y  Y+R V+  L KD + + K+ N +    K       LD  S + R+KLDE+K +E+NRL +L       +K+   L+  E+    D+    ++  HL+      F  +D+++L+    +DL   D  R E+++RYE  K+   R+ L K  +  ++ K  E  ++ +K +  H ++N PGS+DQ KE+W+  D LD + F+ KTFFNLHD N DG +D  E+EA+   ++EKIY+ A    D  E   E ++MR+H+M +VD +KD L++L+E I  T +K+F    EW+T
Sbjct:    6 HCCLILALWACLDALPVAVDKTKVSPPAEAQEPPENADTGLHYDRYLREVIDFLEKDPHFREKLHNTDMEDIKQGKLAKELDFVSHNVRTKLDELKRQEVNRLRTL-------IKAKQDLN-GEKGMTVDHQALLKQFEHLNHMNPHTFEVEDLDRLIKSATNDLENFDKERHEEFKRYEMMKEHERREHL-KTMNEEERKKEEEHYEEMKKKHADHPKLNHPGSQDQLKEVWEEADGLDPNDFDPKTFFNLHDTNGDGYFDEQELEALFTKELEKIYDPAQEEDDMVEMEEERLRMREHVMNEVDTNKDRLVSLEEFITATNKKEFLEPDEWET 331

[Close]

Query:   88 YCTFILYF-AVLSTFCTSSSSEQIEKFIDELNQKE-------YSEYMRNVMQELLKDKNIQGKIFNQNSTSNKD--FMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKT 1059
            +C  IL   A L     +    ++    +     E       Y  Y+R V+  L KD + + K+ N +    K       LD  S + R+KLDE+K +E+NRL +L       +K+   L+  E+    D+    ++  HL+      F  +D+++L+    +DL   D  R E+++RYE  K+   R+ L K  +  ++ K  E  ++ +K +  H ++N PGS+DQ KE+W+  D LD + F+ KTFFNLHD N DG +D  E+EA+   ++EKIY+ A    D  E   E ++MR+H+M +VD +KD L++L+E I  T +K+F    EW+T
Sbjct:    6 HCCLILALWACLDALPVAVDKTKVSPPAEAQEPPENADTGLHYDRYLREVIDFLEKDPHFREKLHNTDMEDIKQGKLAKELDFVSHNVRTKLDELKRQEVNRLRTL-------IKAKQDLN-GEKGMTVDHQALLKQFEHLNHMNPHTFEVEDLDRLIKSATNDLENFDKERHEEFKRYEMMKEHERREHL-KTMNEEERKKEEEHYEEMKKKHADHPKLNHPGSQDQLKEVWEEADGLDPNDFDPKTFFNLHDTNGDGYFDEQELEALFTKELEKIYDPAQEEDDMVEMEEERLRMREHVMNEVDTNKDRLVSLEEFITATNKKEFLEPDEWET 331

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Zebrafish
Match: nucb1 (nucleobindin 1 [Source:ZFIN;Acc:ZDB-GENE-060825-222])

HSP 1 Score: 121.324 bits (303), Expect = 5.919e-30
Identity = 96/293 (32.76%), Postives = 160/293 (54.61%), Query Frame = 1

Query:  190 YSEYMRNVMQELLKDKNIQGKIFNQNS--TSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKT 1059
            Y  Y+R V++ L  D + + K+   N+    N      LDL     R++LDE+K +E++RL        RML  +   S N +    D+ +  ++  HL     + F   D+E L++    DL   D  R E+++RYE  K+   R+E  K  D  ++ K  + + + ++ +R+H ++N PGS DQ +E+W+  D LD   FN KTFF LHD N DG  D+ E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD + D L++L+E +  T +++  +  EW+T
Sbjct:   44 YDRYLREVIEVLETDPHFREKLQTANTEDIKNGRLSKELDLVGHHVRTRLDELKRQEVSRL--------RMLLKAKLDSTNAQSVQMDHASLLKQFEHLDPHNQNTFEAKDLELLIATATKDLENYDAERHEEFKRYEMLKEHE-RREYLKSLDQEKREKEEKRMDELKEKHRQHPKVNSPGSVDQLREVWEETDGLDPQEFNPKTFFKLHDTNSDGVLDVQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMQNVDVNHDRLVSLEEFLKSTEKRELNNQKEWET 327

[Close]

Query:  190 YSEYMRNVMQELLKDKNIQGKIFNQNS--TSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKT 1059
            Y  Y+R V++ L  D + + K+   N+    N      LDL     R++LDE+K +E++RL        RML  +   S N +    D+ +  ++  HL     + F   D+E L++    DL   D  R E+++RYE  K+   R+E  K  D  ++ K  + + + ++ +R+H ++N PGS DQ +E+W+  D LD   FN KTFF LHD N DG  D+ E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD + D L++L+E +  T +++  +  EW+T
Sbjct:   44 YDRYLREVIEVLETDPHFREKLQTANTEDIKNGRLSKELDLVGHHVRTRLDELKRQEVSRL--------RMLLKAKLDSTNAQSVQMDHASLLKQFEHLDPHNQNTFEAKDLELLIATATKDLENYDAERHEEFKRYEMLKEHE-RREYLKSLDQEKREKEEKRMDELKEKHRQHPKVNSPGSVDQLREVWEETDGLDPQEFNPKTFFKLHDTNSDGVLDVQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMQNVDVNHDRLVSLEEFLKSTEKRELNNQKEWET 327

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BLAST of Nucleobindin 2 vs. Ensembl Zebrafish
Match: nucb2a (nucleobindin 2a [Source:ZFIN;Acc:ZDB-GENE-030826-14])

HSP 1 Score: 118.627 bits (296), Expect = 7.568e-29
Identity = 98/297 (33.00%), Postives = 167/297 (56.23%), Query Frame = 1

Query:  190 YSEYMRNVMQELLKDKNIQGKIFNQNSTSNKD--FMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEES 1071
            Y  Y+R V+  L KD++ + K+ N +    K       LD  S   RSKLDE+K +E++RL +L ++ K+ ++    ++        D+    ++  +L+      F  +D+++L+     DL   D  R E++++YE  K+   R+ L+   D   + K  E  ++ +K +  H ++N PGS+DQ KE+W+  D LD   F+ KTFFNLHD N DG +D  E+E++   ++EKIY+  N   D  E   E ++MR+H+M +VD++KD L++L E +V T +K+F     W+T E++
Sbjct:   53 YDRYLREVIDFLEKDQHFREKLHNTDMEDIKQGKLAKELDFVSHHVRSKLDELKRQEVSRLRTL-IKAKQDIEGGNDIA-------VDHQALLKQFEYLNHMNPHTFEVEDLDRLIKSATKDLENYDKERHEEFKKYEMMKEHERREHLKTL-DEDGRKKEEEHYEEMKKKHADHPKVNHPGSKDQLKEVWEEADGLDPEDFDPKTFFNLHDTNGDGFFDEQELESLFTKELEKIYDPTNEEDDMVEMEEERLRMREHVMNEVDSNKDRLVSLDEFLVATKKKEFLEPDSWETLEQN 340

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Xenopus
Match: nucb1 (nucleobindin 1 [Source:NCBI gene;Acc:407884])

HSP 1 Score: 119.013 bits (297), Expect = 5.778e-29
Identity = 84/248 (33.87%), Postives = 138/248 (55.65%), Query Frame = 1

Query:  337 EIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEE--IXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEES 1071
            E+K +E++RL        RML  +   +  E +   D+++  ++  HL       F   D+E L+     DL   D  R E+++RYE  K+   R+ L+   +   + +++EE   ++ +K +++H ++N PGS DQ KE+W+  D LD + FN KTFF LHD N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD + D L+TL E +  T RK+F     W+T +E+
Sbjct:   77 ELKRQEVSRL--------RMLIKAKMDATMEENVQIDHMSLLKQFEHLDPQNQHTFEARDLELLIQAATKDLENYDAARHEEFKRYEMMKEHERREYLKSLDE---EKRKMEEAHFEEMKKKHKEHPKVNVPGSIDQLKEVWEETDGLDPNEFNPKTFFKLHDTNGDGVLDEQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMKNVDANHDRLVTLDEFLKSTERKEFNEADGWETVDET 313

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Xenopus
Match: nucb1 (nucleobindin 1 [Source:NCBI gene;Acc:407884])

HSP 1 Score: 118.242 bits (295), Expect = 6.245e-29
Identity = 100/299 (33.44%), Postives = 160/299 (53.51%), Query Frame = 1

Query:  190 YSEYMRNVMQELLKDKNIQGKI--FNQNSTSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEE--IXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEES 1071
            Y  Y+R V+  L  D + + K+   N +   +      LD  S   R+KLDE+K +E++RL        RML  +   +  E +   D+++  ++  HL       F   D+E L+     DL   D  R E+++RYE  K+   R   E  K   ++ +++EE   ++ +K +++H ++N PGS DQ KE+W+  D LD + FN KTFF LHD N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD + D L+TL E +  T RK+F     W+T +E+
Sbjct:   44 YDRYLREVIDVLETDGHFREKLQAANADDIKSGKLSKELDFVSHHVRTKLDELKRQEVSRL--------RMLIKAKMDATMEENVQIDHMSLLKQFEHLDPQNQHTFEARDLELLIQAATKDLENYDAARHEEFKRYEMMKEHERR---EYLKSLDEEKRKMEEAHFEEMKKKHKEHPKVNVPGSIDQLKEVWEETDGLDPNEFNPKTFFKLHDTNGDGVLDEQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMKNVDANHDRLVTLDEFLKSTERKEFNEADGWETVDET 331

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Xenopus
Match: nucb1 (nucleobindin 1 [Source:NCBI gene;Acc:407884])

HSP 1 Score: 118.627 bits (296), Expect = 9.418e-29
Identity = 84/248 (33.87%), Postives = 138/248 (55.65%), Query Frame = 1

Query:  337 EIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEE--IXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEES 1071
            E+K +E++RL        RML  +   +  E +   D+++  ++  HL       F   D+E L+     DL   D  R E+++RYE  K+   R+ L+   +   + +++EE   ++ +K +++H ++N PGS DQ KE+W+  D LD + FN KTFF LHD N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD + D L+TL E +  T RK+F     W+T +E+
Sbjct:   95 ELKRQEVSRL--------RMLIKAKMDATMEENVQIDHMSLLKQFEHLDPQNQHTFEARDLELLIQAATKDLENYDAARHEEFKRYEMMKEHERREYLKSLDE---EKRKMEEAHFEEMKKKHKEHPKVNVPGSIDQLKEVWEETDGLDPNEFNPKTFFKLHDTNGDGVLDEQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMKNVDANHDRLVTLDEFLKSTERKEFNEADGWETVDET 331

[Close]

Query:  337 EIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEE--IXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEES 1071
            E+K +E++RL        RML  +   +  E +   D+++  ++  HL       F   D+E L+     DL   D  R E+++RYE  K+   R+ L+   +   + +++EE   ++ +K +++H ++N PGS DQ KE+W+  D LD + FN KTFF LHD N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD + D L+TL E +  T RK+F     W+T +E+
Sbjct:   95 ELKRQEVSRL--------RMLIKAKMDATMEENVQIDHMSLLKQFEHLDPQNQHTFEARDLELLIQAATKDLENYDAARHEEFKRYEMMKEHERREYLKSLDE---EKRKMEEAHFEEMKKKHKEHPKVNVPGSIDQLKEVWEETDGLDPNEFNPKTFFKLHDTNGDGVLDEQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMKNVDANHDRLVTLDEFLKSTERKEFNEADGWETVDET 331

[Close]

Query:  337 EIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEE--IXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEES 1071
            E+K +E++RL        RML  +   +  E +   D+++  ++  HL       F   D+E L+     DL   D  R E+++RYE  K+   R+ L+   +   + +++EE   ++ +K +++H ++N PGS DQ KE+W+  D LD + FN KTFF LHD N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD + D L+TL E +  T RK+F     W+T +E+
Sbjct:   95 ELKRQEVSRL--------RMLIKAKMDATMEENVQIDHMSLLKQFEHLDPQNQHTFEARDLELLIQAATKDLENYDAARHEEFKRYEMMKEHERREYLKSLDE---EKRKMEEAHFEEMKKKHKEHPKVNVPGSIDQLKEVWEETDGLDPNEFNPKTFFKLHDTNGDGVLDEQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMKNVDANHDRLVTLDEFLKSTERKEFNEADGWETVDET 331

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Mouse
Match: Nucb1 (nucleobindin 1 [Source:MGI Symbol;Acc:MGI:97388])

HSP 1 Score: 127.102 bits (318), Expect = 6.850e-32
Identity = 86/247 (34.82%), Postives = 140/247 (56.68%), Query Frame = 1

Query:  337 EIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIE-WKTSEES 1071
            E+K +E++RL        RML  +   +K E +   D++   ++  HL       F   D+E L+     DL   D    E+++RYE  K+   R+ LE   +  Q+ +   ++++ ++ +R+H ++N PGS+ Q KE+W+ +D LD + FN KTFF LHD+N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD ++D L+TL+E +  T RK+F    E WKT E S
Sbjct:  101 ELKRQEVSRL--------RMLLKAKMDAKQEPNLQVDHMNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGE-EQRKEAERKLQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWKTVEMS 338

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Mouse
Match: Nucb1 (nucleobindin 1 [Source:MGI Symbol;Acc:MGI:97388])

HSP 1 Score: 127.102 bits (318), Expect = 7.195e-32
Identity = 86/247 (34.82%), Postives = 140/247 (56.68%), Query Frame = 1

Query:  337 EIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIE-WKTSEES 1071
            E+K +E++RL        RML  +   +K E +   D++   ++  HL       F   D+E L+     DL   D    E+++RYE  K+   R+ LE   +  Q+ +   ++++ ++ +R+H ++N PGS+ Q KE+W+ +D LD + FN KTFF LHD+N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD ++D L+TL+E +  T RK+F    E WKT E S
Sbjct:  101 ELKRQEVSRL--------RMLLKAKMDAKQEPNLQVDHMNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGE-EQRKEAERKLQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWKTVEMS 338

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Mouse
Match: Nucb1 (nucleobindin 1 [Source:MGI Symbol;Acc:MGI:97388])

HSP 1 Score: 115.931 bits (289), Expect = 2.884e-29
Identity = 69/177 (38.98%), Postives = 110/177 (62.15%), Query Frame = 1

Query:  544 DLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIE-WKTSEES 1071
            DL   D    E+++RYE  K+   R+ LE   +  Q+ +   ++++ ++ +R+H ++N PGS+ Q KE+W+ +D LD + FN KTFF LHD+N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD ++D L+TL+E +  T RK+F    E WKT E S
Sbjct:   30 DLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEE-QRKEAERKLQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWKTVEMS 205

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Mouse
Match: Nucb2 (nucleobindin 2 [Source:MGI Symbol;Acc:MGI:1858179])

HSP 1 Score: 114.39 bits (285), Expect = 1.265e-27
Identity = 80/241 (33.20%), Postives = 133/241 (55.19%), Query Frame = 1

Query:  337 EIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKT 1059
            E+K +E+ RL        RML  + KL   +   M  +L   +  HL+    + F   D++ L+     DL   D  R E++++YE  K+   R+E  K     ++ +   + ++ ++ +  H ++N PGS+DQ KE+W+  D LD + F+ KTFF LHD+N DG  D  E+EA+   ++EK+Y   N   D  E   E ++MR+H+M ++DN+KD L+TL+E +  T +K+F     W+T
Sbjct:  105 ELKRQEVGRL--------RMLIKA-KLDALQDTGMNHHLLLKQFEHLNHQNPNTFESRDLDMLIKAATADLEQYDRTRHEEFKKYEMMKEHE-RREYLKTLSEEKRKEEESKFEEMKRKHEDHPKVNHPGSKDQLKEVWEETDGLDPNDFDPKTFFKLHDVNNDGFLDEQELEALFTRELEKVYNPQNAEDDMIEMEEERLRMREHVMSEIDNNKDRLVTLEEFLRATEKKEFLEPDSWET 335

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Mouse
Match: Nucb2 (nucleobindin 2 [Source:MGI Symbol;Acc:MGI:1858179])

HSP 1 Score: 114.39 bits (285), Expect = 1.368e-27
Identity = 80/241 (33.20%), Postives = 133/241 (55.19%), Query Frame = 1

Query:  337 EIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKT 1059
            E+K +E+ RL        RML  + KL   +   M  +L   +  HL+    + F   D++ L+     DL   D  R E++++YE  K+   R+E  K     ++ +   + ++ ++ +  H ++N PGS+DQ KE+W+  D LD + F+ KTFF LHD+N DG  D  E+EA+   ++EK+Y   N   D  E   E ++MR+H+M ++DN+KD L+TL+E +  T +K+F     W+T
Sbjct:  105 ELKRQEVGRL--------RMLIKA-KLDALQDTGMNHHLLLKQFEHLNHQNPNTFESRDLDMLIKAATADLEQYDRTRHEEFKKYEMMKEHE-RREYLKTLSEEKRKEEESKFEEMKRKHEDHPKVNHPGSKDQLKEVWEETDGLDPNDFDPKTFFKLHDVNNDGFLDEQELEALFTRELEKVYNPQNAEDDMIEMEEERLRMREHVMSEIDNNKDRLVTLEEFLRATEKKEFLEPDSWET 335

[Close]

BLAST of Nucleobindin 2 vs. UniProt/SwissProt
Match: sp|Q63083|NUCB1_RAT (Nucleobindin-1 OS=Rattus norvegicus OX=10116 GN=Nucb1 PE=1 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 3.778e-31
Identity = 85/245 (34.69%), Postives = 139/245 (56.73%), Query Frame = 1

Query:  337 EIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIE-WKTSE 1065
            E+K +E++RL        RML  +   +K E +   D++   ++  HL       F   D+E L+     DL   D    E+++RYE  K+   R+ LE   +  Q+ +   ++++ ++ +R+H ++N PGS+ Q KE+W+ +D LD + FN KTFF LHD+N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD ++D L+TL+E +  T RK+F    E WKT E
Sbjct:  101 ELKRQEVSRL--------RMLLKAKMDAKQEPNLQVDHMNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGE-EQRKEAERKLQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGETAEGWKTVE 336

[Close]

BLAST of Nucleobindin 2 vs. UniProt/SwissProt
Match: sp|Q02819|NUCB1_MOUSE (Nucleobindin-1 OS=Mus musculus OX=10090 GN=Nucb1 PE=1 SV=2)

HSP 1 Score: 127.102 bits (318), Expect = 4.791e-31
Identity = 86/247 (34.82%), Postives = 140/247 (56.68%), Query Frame = 1

Query:  337 EIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIE-WKTSEES 1071
            E+K +E++RL        RML  +   +K E +   D++   ++  HL       F   D+E L+     DL   D    E+++RYE  K+   R+ LE   +  Q+ +   ++++ ++ +R+H ++N PGS+ Q KE+W+ +D LD + FN KTFF LHD+N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD ++D L+TL+E +  T RK+F    E WKT E S
Sbjct:  101 ELKRQEVSRL--------RMLLKAKMDAKQEPNLQVDHMNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGE-EQRKEAERKLQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWKTVEMS 338

[Close]

BLAST of Nucleobindin 2 vs. UniProt/SwissProt
Match: sp|Q5R4U1|NUCB1_PONAB (Nucleobindin-1 OS=Pongo abelii OX=9601 GN=NUCB1 PE=2 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 6.287e-30
Identity = 87/247 (35.22%), Postives = 138/247 (55.87%), Query Frame = 1

Query:  337 EIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKS--KRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIE-WKTSE 1065
            E+K +E++RL        RML  +   ++ + +   D+L   ++  HL       F   D+E L+     DL   D    E+++RYE  K+   R+ LE   +  +K   +RLEE    ++ +R+H ++N PGS+ Q KE+W+ +D LD + FN KTFF LHD+N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD ++D L+TL E +  T RK+F    E W+T E
Sbjct:  102 ELKRQEVSRL--------RMLLKAKMDAEQDPNVQVDHLNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERRLEE---QQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLGEFLASTQRKEFGDTGEGWETVE 337

[Close]

BLAST of Nucleobindin 2 vs. UniProt/SwissProt
Match: sp|Q02818|NUCB1_HUMAN (Nucleobindin-1 OS=Homo sapiens OX=9606 GN=NUCB1 PE=1 SV=4)

HSP 1 Score: 123.25 bits (308), Expect = 1.109e-29
Identity = 86/247 (34.82%), Postives = 139/247 (56.28%), Query Frame = 1

Query:  337 EIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKS--KRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIE-WKTSE 1065
            E+K +E++RL        RML  +   ++ + +   D+L   ++  HL       F   D+E L+     DL   D    E+++RYE  K+   R+ LE   +  +K   ++LEE    ++ +R+H ++N PGS+ Q KE+W+ +D LD + FN KTFF LHD+N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD ++D L+TL+E +  T RK+F    E W+T E
Sbjct:  102 ELKRQEVSRL--------RMLLKAKMDAEQDPNVQVDHLNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEE---QQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWETVE 337

[Close]

BLAST of Nucleobindin 2 vs. UniProt/SwissProt
Match: sp|Q0P569|NUCB1_BOVIN (Nucleobindin-1 OS=Bos taurus OX=9913 GN=NUCB1 PE=1 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.470e-29
Identity = 84/242 (34.71%), Postives = 135/242 (55.79%), Query Frame = 1

Query:  337 EIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKS--KRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEW 1053
            E+K +E++RL        RML  +   ++ E +   D+L   ++  HL       F   D+E L+     DL   D    E+++RYE  K+   R+ LE   +  +K   ++LEE    ++ +R+H ++N PGS+ Q KE+W+ +D LD + FN KTFF LHD+N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD ++D L+TL+E +  T RK+F    E 
Sbjct:   99 ELKRQEVSRL--------RMLLKAKMDAQQEPNIQLDHLNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEE---QQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEDDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEG 329

[Close]

BLAST of Nucleobindin 2 vs. TrEMBL
Match: A0A267H5F8 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig004318g1 PE=4 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 1.507e-37
Identity = 117/346 (33.82%), Postives = 189/346 (54.62%), Query Frame = 1

Query:   76 AFMCYCTFILYFA--VLSTFCTSSSSEQIEKFIDELNQKEYSEYMRNVMQELLKDKNIQGKIFNQNSTSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLT---------SYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKD-WRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDAN-GTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEESD 1074
            A + +C     FA  V+     +  +++     D L+   Y+ Y+R V Q L  +K    ++  QN +   D    L+    S R++LDEIK  E++RLN+L+    +ML++    + N   E    +          + ++ HL       F   D+EKL+ Q+  D+  VD  ++EQY++YE  K+  RLRK L K  D   +    +E ++ +K +++H++I+ PGS +Q ++ W   D++D   FN KTFF LHD+N DG+ D+NEIEA+   ++EK Y   N  T +  EK HE+ +MRQ +++KVD +KD +I+  E   +T   DFK + +WKTSEE D
Sbjct:   13 AAILFCCLTGCFAPPVVKKPAPTVVAKKPGPADDLLSHLPYANYVREVSQILESNKEFAKRV--QNLSEAGDLASQLEFVDHSVRTRLDEIKRNEVDRLNALE----KMLEAKKAAAGNPMREAGVPIHPDVFNMDQETIKKAHLDHENPHSFEASDLEKLIHQVVSDMHRVDEAKQEQYKQYEMMKEHLRLRK-LHKINDTKAREAAEKEYQEEKKKHKQHEKIHHPGSSEQLRDAWADEDHMDKDSFNPKTFFLLHDVNGDGHLDVNEIEALFLKELEKAYNRTNDTTVNALEKQHEMERMRQEVLDKVDTNKDMMISWDEFERWTKNPDFKKDDDWKTSEEDD 351

[Close]

BLAST of Nucleobindin 2 vs. TrEMBL
Match: B7Q350 (Niemann-Pick type C1 domain-containing protein, putative OS=Ixodes scapularis OX=6945 GN=8035180 PE=4 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 2.628e-37
Identity = 95/296 (32.09%), Postives = 167/296 (56.42%), Query Frame = 1

Query:  187 EYSEYMRNVMQELLKDKNIQGKIFNQ--NSTSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEE 1068
            EY  Y++ V++ L  D     K+ N   +   + D    L+    S R++LDE+K  E++RL   +L +K M +    L   +RD++   L      H+       F  DD++KL+     DL  +D  R+E+++ YE QK+   R +LE   +  +K K  +  ++ ++ ++ H  ++EPGS+ Q +E W+  D+++   F+  TFF +HDLN DG  D +EIEA++ P+++K+Y+  N   DP E+  EI +MR+H++ +V ++KD +I+ +E +  T RKDF+ +  WK  +E
Sbjct: 1211 EYDRYLQEVVRALEDDAEFAAKLRNVSIDDIKSGDVAHQLEFVKHSVRNRLDELKRIEVDRLR--RLTVKAMERKEFGL---DRDQVKMPL------HVDYQNPHSFEIDDLKKLIVTATKDLEKLDEKRKEEFKEYEMQKELEYRHKLENMTEE-EKKKEQQHHEELKRKHKDHPAVHEPGSKPQLEETWEKQDHMNKEDFDPNTFFAMHDLNGDGQLDQDEIEAILTPEVKKVYDPNNEEDDPRERQEEIQRMREHVVHEVSSNKDGMISHEEFMDMTQRKDFERDDGWKGLDE 1494

[Close]

BLAST of Nucleobindin 2 vs. TrEMBL
Match: A0A267FYX5 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009833g1 PE=4 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 3.737e-37
Identity = 117/346 (33.82%), Postives = 190/346 (54.91%), Query Frame = 1

Query:   76 AFMCYCTFILYFA--VLSTFCTSSSSEQIEKFIDELNQKEYSEYMRNVMQELLKDKNIQGKIFNQNSTSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLT---------SYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKD-WRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDAN-GTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEESD 1074
            A + +C     FA  V+     +  +++     D L+   Y+ Y+R V Q L  +K    ++  QN +   D    L+    S R++LDEIK +E++RLN+L+    +ML++    + N   E    +          + ++ HL       F   D+EKL+ Q+  D+  VD  ++EQY++YE  K+  RLRK L K  D   +    +E ++ +K +++H++I+ PGS +Q ++ W   D++D   FN KTFF LHD+N DG+ D+NEIEA+   ++EK Y   N  T +  EK HE+ +MRQ +++KVD +KD +I+  E   +T   DFK + +WKTSEE D
Sbjct:   12 AAILFCCLTGCFAPPVVKKPAPTVVAKKPGPADDLLSHLPYANYVREVSQILESNKEFAKRV--QNLSEAGDLASQLEFVDHSVRTRLDEIKRKEVDRLNALE----KMLEAKKAAAGNPMREAEVPIHPDVFNMDQETIKKAHLDHENPHSFESSDLEKLIRQVVSDMHRVDEAKQEQYKQYEMMKEHLRLRK-LHKINDTKAREVAEKEYQEEKKKHKQHEKIHHPGSSEQLRDAWADEDHMDKDSFNPKTFFLLHDVNGDGHLDVNEIEALFLKELEKAYNRTNDTTVNALEKQHEMERMRQEVLDKVDTNKDMMISWDEFERWTKNPDFKKDDDWKTSEEDD 350

[Close]

BLAST of Nucleobindin 2 vs. TrEMBL
Match: A0A1I8HPU3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 1.999e-35
Identity = 113/351 (32.19%), Postives = 186/351 (52.99%), Query Frame = 1

Query:   76 AFMCYCTFILYFAVL-----STFCTSSSSEQIEKFIDELNQKEYSEYMRNVMQELLKDKNIQGKI--FNQNSTSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLT---------SYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKD-WRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDAN-GTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEESD 1074
            A + +C     FA       +    +      +  +   +  +Y +Y+  VM  L  DK+ + +   F  ++    D    L+    S R++LDEIK  E++RLN+L+    +ML++    + N   E    +          + ++ HL       F   D+EKL+ Q+  D+  VD  ++EQY++YE  K+  RLRK L K  D   +    +E ++ +K +++H++I+ PGS +Q ++ W   D++D   FN KTFF LHD+N DG+ D+NEIEA+   ++EK Y   N  T +  EK HE+ +MRQ +++KVD +KD +I+  E   +T   DFK + +WKTSEE D
Sbjct:   10 AAILFCCLTGCFAPPVVKKPAPTVVAKKPGPADDLLKAYSPDDYKKYVEEVMAHLHMDKHFKSEWEKFKNSTHKAGDLASQLEFVDHSVRTRLDEIKRNEVDRLNALE----KMLEAKKAAAGNPMREAGVPIHPDVFNMDQETIKKAHLDHENPHSFEASDLEKLIHQVVSDMHRVDEAKQEQYKQYEMMKEHLRLRK-LHKINDTKAREAAEKEYQEEKKKHKQHEKIHHPGSSEQLRDAWADEDHMDKDSFNPKTFFLLHDVNGDGHLDVNEIEALFLKELEKAYNRTNDTTVNALEKQHEMERMRQEVLDKVDTNKDMMISWDEFERWTKNPDFKKDDDWKTSEEDD 355

[Close]

BLAST of Nucleobindin 2 vs. TrEMBL
Match: A0A267DJS5 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034413g1 PE=4 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 5.560e-35
Identity = 111/352 (31.53%), Postives = 194/352 (55.11%), Query Frame = 1

Query:   43 LTATQNFFFI*AFMCY-CTFILYFAVLSTFCTSSSSEQIEKFIDELNQKEYSEYMRNVMQELLKDKNIQGKIFNQNSTSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSY-----RRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDAN-GTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEESDQ 1077
            +T +   F I AF+   CT   +   +S     + SE++   +  L    Y +Y+  V+  +  DK    ++ + N +   +  + L+ AS S R++LDEIK +E+ RLN L+  L+  L       K  R +    L S      +R HL  +    F   D++KL++Q+  D+  VD  RE +Y+RYE  K+   R  L + KD   +    ++++  R+ +R+H+R+++PGS +Q KE W+  D +D   F+ K FF LHD+N DG+ D+NEIEA+   +++K+Y   N  T D  E+ HE+ +MR+ ++ ++D +KD +++ +E   + + ++   + EWKT+EESD+
Sbjct:    3 MTPSIKLFLISAFLLISCT---HSVPVSKEEKKAESERLPDNV--LPDVSYRQYLSEVISAMESDKEFANRLRSMNDSD--EMANQLEFASHSVRNRLDEIKRQEVERLNELEKALEDKLALKANPGKASRAKFRFGLQSLDEATIKRAHLDHSNPHTFEIADLQKLINQVTQDIDQVDQAREAEYKRYEMGKEHSRRARLRRIKDKKAREAAEQQMRAERERHRRHERVHQPGSREQLKEAWEKGDRMDRDSFDPKAFFMLHDINGDGHMDVNEIEALFWKELDKVYSKENDTTVDRVERQHEMERMRRAVLMRLDKNKDRMVSWEEFNAWISSQEANKDEEWKTAEESDE 347

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Cavefish
Match: nucb1 (nucleobindin-2-like [Source:NCBI gene;Acc:103046537])

HSP 1 Score: 115.931 bits (289), Expect = 4.832e-28
Identity = 100/293 (34.13%), Postives = 162/293 (55.29%), Query Frame = 1

Query:  190 YSEYMRNVMQELLKDKNIQGKIFNQNS--TSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKT 1059
            Y  Y+R V++ L  D + + K+   N+    N      LDL     R++LDE+K +E++RL        RML  +   S N +    D+++  ++  HL     + F   D+E L++    DL   D  R E+++RYE  K+   R+E  K  D  ++ +  + I+  R+ +R+H ++N PGS DQ +E+W+  D LD   FN KTFF LHD N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+ VD ++D L++L+E I  T +K+F +  EW+T
Sbjct:   49 YDRYLREVIEVLETDPHFREKLQTANTEDIKNGRLSKELDLVGHHVRTRLDELKRQEVSRL--------RMLLKAKLDSTNTQSVQMDHVSLLKQFEHLDPHNQNTFEAKDLELLIAAATKDLENYDAERHEEFKRYEMLKEHE-RREYLKSLDQEKREREEKRIEDLREKHRQHPKVNAPGSVDQLREVWEETDGLDPQEFNPKTFFKLHDTNGDGLLDEQELEALFTKELEKVYDPKNEEDDMMEMEEERLRMREHVMKNVDLNRDRLVSLEEFIKSTEKKEFNNAKEWET 332

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Cavefish
Match: nucb1 (nucleobindin-2-like [Source:NCBI gene;Acc:103046537])

HSP 1 Score: 96.6709 bits (239), Expect = 1.413e-21
Identity = 93/281 (33.10%), Postives = 149/281 (53.02%), Query Frame = 1

Query:  190 YSEYMRNVMQELLKDKNIQGKIFNQNS--TSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTA 1023
            Y  Y+R V++ L  D + + K+   N+    N      LDL     R++LDE+K +E++RL        RML  +   S N +    D+++  ++  HL     + F   D+E L++    DL   D  R E+++RYE  K+   R+E  K  D  ++ +  + I+  R+ +R+H ++N PGS DQ +E+W+  D LD   FN KTFF LHD N DG  D  E+EA+   ++EK+Y+  N   D  E   E ++MR+H+M+   +DK  + T  EL  + A
Sbjct:   49 YDRYLREVIEVLETDPHFREKLQTANTEDIKNGRLSKELDLVGHHVRTRLDELKRQEVSRL--------RMLLKAKLDSTNTQSVQMDHVSLLKQFEHLDPHNQNTFEAKDLELLIAAATKDLENYDAERHEEFKRYEMLKEHE-RREYLKSLDQEKREREEKRIEDLREKHRQHPKVNAPGSVDQLREVWEETDGLDPQEFNPKTFFKLHDTNGDGLLDEQELEALFTKELEKVYDPKNEEDDMMEMEEERLRMREHVMKNTLDDKQPVYTEAELQRFEA 320

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Cavefish
Match: nucb2a (nucleobindin 2a [Source:ZFIN;Acc:ZDB-GENE-030826-14])

HSP 1 Score: 48.521 bits (114), Expect = 8.976e-6
Identity = 85/348 (24.43%), Postives = 150/348 (43.10%), Query Frame = 1

Query:   97 FILYFAVLSTFCTSSSSEQI----------EKFIDELNQK-----EYSEYMRNVMQELLKDKNIQGKIFNQNSTSNKD--FMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRRT--HLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEI-WKSVDNLDISY---FNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEES 1071
            FI    VL++   SS +  I          E+ + E  Q       Y  Y+R V+  L KD++ + K+ N +    K       LD  S   R+KLDE+K +E++RL +L            K    E + MA +  +  +   +L+      F  +D+++L+     DL   D  R E++++YE  K+   R+ L+   +  +K +                       E+ ++E+  K  D+  +++   F +K  + L +L                   EKIY+  N   D  E   E ++MR+H+M +VD +KD L++L E +  T +K+F     W+T E++
Sbjct:    7 FITSCLVLASLLFSSEAVPISVDKTKVKLPEETVQEPPQSVDTGLHYDRYLREVIDFLEKDQHFREKLHNTDMEDIKQGKLAKELDFVSHHVRTKLDELKRQEVSRLRTL---------IKAKQDVEEGNGMAVDHQALLKQFEYLNHMNPHTFEVEDLDRLIKSATSDLENFDKERHEEFKKYEMMKEHERREHLKTLDEEGRKKE-----------------------EEHYEEMKKKHADHPKVNHPVRFILKLIYTLTEL-------------------EKIYDPTNEEDDMVEMEEERLRMREHVMNEVDANKDRLVSLDEFLTATKKKEFLEPDSWETLEQN 303

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Sea Lamprey
Match: nucb2b (nucleobindin 2b [Source:ZFIN;Acc:ZDB-GENE-030131-4775])

HSP 1 Score: 65.4698 bits (158), Expect = 2.641e-12
Identity = 61/217 (28.11%), Postives = 99/217 (45.62%), Query Frame = 1

Query:  190 YSEYMRNVMQELLKDKNIQGK--------IFNQNSTSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLH 813
            Y  Y+R V+  L KD + + K        I N   +   DF+     +         E+K +E+ RL        RML  +   +   +D   D+ +  ++  HL       F   D+EKL+     DL   D  R E+++RYE  K+   R+E  K  D  ++ +  +     ++ ++ H ++N PGS+DQ +E+W+  D LD   F  KTFF LH
Sbjct:   60 YDRYLREVIDVLEKDTHFKQKLETADIEDIKNGRLSKELDFVSHHVRSKLD------ELKRQEVARL--------RMLIKAKMDAAQGKDLRMDHQSLLKQFEHLDHNNPHSFEPQDLEKLIKTATKDLENYDAARHEEFKRYEMLKEHE-RREFLKTLDEERRQEEEKRFLDMKQKHKDHPKVNHPGSKDQLQEVWEESDGLDPEQFEPKTFFKLH 261

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Nematostella
Match: EDO43098 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZI2])

HSP 1 Score: 100.138 bits (248), Expect = 2.736e-23
Identity = 79/299 (26.42%), Postives = 157/299 (52.51%), Query Frame = 1

Query:  187 EYSEYMRNVMQELLKDKNIQGKIFNQNSTSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYR-RTHLHSTEDSK----FTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEE 1068
            EY+ Y+R V++ L KD++   K+ N    S+ D        +        +++S+ ++ L   +++ +RM+          R +M D+L   + R + +   D +    F  DD +KL+ +   ++   D  R E+++++E QK+   + +++   +  +K       +   K + + K+++ PGS+ Q +++W+  D LD   F+ KTFF LHD+N DG  D  E+EA+   ++ K+Y   +   DP+E+  EI +MR+H+M ++D DKD  ++  E +  +  ++F  +  WK+ E+
Sbjct:   43 EYARYLRQVIEILEKDEDYVRKLMN---ASDDDL--RSGRIAEDIDLVKHDVRSK-LDELKRQEVERQRMI----------RRQMNDHLNGIKEREYWNPLFDDENPDFFGADDFKKLLWKHHEEMDKQDRERREEFKKHEMQKEHERKIKMKDMDEKHRKEAEEHFRELQEKHHNQSKQLHHPGSKAQLEQVWEESDGLDAKDFDPKTFFKLHDVNGDGFLDTGELEALFVKEVTKLYNPKDEDYDPKERDEEISRMREHVMNEIDKDKDGFVSQDEFLQSSKGEEFDKDDGWKSVED 325

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Medaka
Match: nucb2a (nucleobindin-2 [Source:NCBI gene;Acc:101163155])

HSP 1 Score: 125.176 bits (313), Expect = 3.432e-31
Identity = 100/297 (33.67%), Postives = 167/297 (56.23%), Query Frame = 1

Query:  190 YSEYMRNVMQELLKDKNIQGKIFNQNSTSNKD--FMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEES 1071
            Y  Y+R V+  L KD++ + K+ N +    K       LD  S   R+KLDE+K +E+NRL +L ++ K+ ++    ++        D+    ++  +L+      F  +D+++L+     DL   D  R E+++RYE  K+   R+ L+   D  ++ K  E  ++ RK +  H ++N PGSE+Q KE+W+  D LD   F+ KTFFNLHD N DG +D  E+EA+   ++EKIY+  N   D  E   E ++MR+H+M +VD +KD L+++ E +V T +K+F     W+T E++
Sbjct:   63 YDRYLREVIDFLEKDQHFREKLHNTDMEDIKQGKLAKELDFVSHHVRTKLDELKRQEVNRLRTL-IKAKQDIEGGNDIA-------VDHQALLKQFEYLNHMNPHTFEVEDLDRLIKSATKDLENYDKERHEEFKRYEMMKEHDRREHLKTLNDE-ERKKEEEHYEEMRKKHADHPKVNHPGSENQLKEVWEEADGLDPEDFDPKTFFNLHDTNGDGFFDEQELEALFTKELEKIYDPTNEEDDMVEMEEERLRMREHVMNEVDTNKDRLVSMDEFLVATKKKEFLEPDSWETLEQN 350

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Medaka
Match: nucb1 (nucleobindin-2 [Source:NCBI gene;Acc:101169036])

HSP 1 Score: 119.013 bits (297), Expect = 3.157e-29
Identity = 101/293 (34.47%), Postives = 161/293 (54.95%), Query Frame = 1

Query:  190 YSEYMRNVMQELLKDKNIQGKIFNQNS--TSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRR-THLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKT 1059
            Y  Y+R V++ L  D + + K+   N+    N      LDL     R++LDE+K +E++RL        RML  +   S N +    D+ +  ++  HL     + F   D+E L+S    DL   D  R E+++RYE  K+   R+E  K  D  ++ K  + I++ ++ +R+H ++N PGS DQ +E+W+  D LD   FN KTFF LHD N+DG  D  E+EA+   ++EK+Y   N   D  E   E ++MR+H+M+ VD ++D L+TL+E +  T RK+F +   W+T
Sbjct:   47 YDRYLREVIEVLETDPHFREKLQTANTEDIKNGRLSKELDLVGHHVRTRLDELKRQEVSRL--------RMLLKAKLDSTNTQSLQMDHASLLKQFEHLDPHNQNTFEAKDLELLISAATKDLENYDAERHEEFKRYEMLKEHE-RREYLKSLDQEKREKEEKRIQELKEKHRQHPKVNAPGSVDQLREVWEETDGLDPQEFNPKTFFKLHDTNEDGVLDEQELEALFTKELEKVYNPKNEEDDMMEMEEERLRMREHVMKNVDTNRDRLVTLEEFLKSTERKEFNNAKGWET 330

[Close]

BLAST of Nucleobindin 2 vs. Ensembl Medaka
Match: NUCB2 (nucleobindin 2 [Source:NCBI gene;Acc:101162363])

HSP 1 Score: 118.242 bits (295), Expect = 3.719e-29
Identity = 97/292 (33.22%), Postives = 156/292 (53.42%), Query Frame = 1

Query:  190 YSEYMRNVMQELLKDKNIQGKIFNQNSTSNKD--FMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKT 1059
            Y  Y+R V++ L KD + + K+ N +    K       L+    + RSKLDE+K  EINRL        RML  + K    E + M       +  HL+      F  DD+++L+     DL   D  R +++++YE  K+   R+ L+   +  +K K  +  ++ +K +  H ++N PGSEDQ KE+W+  D LD   F+ KTFF +HD N DG +D +E+EA+   ++EK+Y   N   D  E   E ++M++H+M +VD +KD L++  E +  T ++DF    EW+T
Sbjct:   52 YDRYLREVIEYLEKDPHFREKLKNADMDDIKQGKLSKELNFVQHNFRSKLDELKREEINRL--------RMLIKA-KHDTEEHNAMDHQTLLKQFEHLNHMNPHTFEVDDLDRLIQSATKDLENFDKERHDEFKKYELLKEHERRERLKGMSEEDRK-KAEQHYEEMKKKHANHPKVNHPGSEDQLKEVWQETDGLDPQDFDPKTFFKMHDSNGDGFFDESELEALFTKELEKVYNPDNEEDDMVEMEEERLRMKEHVMNEVDTNKDRLVSFSEFMAATKKEDFAEKDEWET 333

[Close]

BLAST of Nucleobindin 2 vs. Planmine SMEST
Match: SMESG000016720.1 (SMESG000016720.1)

HSP 1 Score: 628.246 bits (1619), Expect = 0.000e+0
Identity = 360/363 (99.17%), Postives = 363/363 (100.00%), Query Frame = 1

Query:   82 MCYCTFILYFAVLSTFCTSSSSEQIEKFIDELNQKEYSEYMRNVMQELLKDKNIQGKIFNQNSTSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEESDQXXXXXXXXXXXXXXXXXXXQSSVVNVDESLN 1170
            MCYCTFILYFAVLSTFCTSSSSEQIEKFIDELNQKEYSEYMRNVMQELLKDKNIQGKIFNQNSTSNKDFMDALDLASSSSR+KLDEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEW+KDWRLRKELEKFKDPVQKSKRLEEIKKSRKSYRKHKRINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEK+DNDKDDLITLQELIVYTARKDFKSNIEWKTSEESDQSIYSEEEKIKFEKELEEISQSSVVNVDESLN
Sbjct:    1 MCYCTFILYFAVLSTFCTSSSSEQIEKFIDELNQKEYSEYMRNVMQELLKDKNIQGKIFNQNSTSNKDFMDALDLASSSSRNKLDEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWKKDWRLRKELEKFKDPVQKSKRLEEIKKSRKSYRKHKRINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKIDNDKDDLITLQELIVYTARKDFKSNIEWKTSEESDQSIYSEEEKIKFEKELEEISQSSVVNVDESLN 363

[Close]

BLAST of Nucleobindin 2 vs. Planmine SMEST
Match: SMESG000017142.1 (SMESG000017142.1)

HSP 1 Score: 298.13 bits (762), Expect = 5.583e-99
Identity = 168/292 (57.53%), Postives = 229/292 (78.42%), Query Frame = 1

Query:  202 MRNVMQELLKDKNIQGKIFNQ-NSTSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEESD 1074
            M++VM EL  D   Q K+ ++  S SN+D++ AL+ ASSS+R+KLDEIK REI RLN  KL+LKR LK +  ++K ERD++  +LT     H+HST++ KFTK+D EKL+SQI+HDLRLVD  RE++YRR+EW+KD  ++K+LE  KDP  KSKRLEE+KK+RK+ R HK +NEPGSE+Q KE+W++ D LD SYFN+KTFFNLHD+NQDG+WDI E+E+++ P++ KIYE  N T DPEEK+HEIMKMR+H MEK+D DKD+++T +E + ++ +K+FK N EWK +EE D
Sbjct:    1 MQSVMTELGTDIEFQNKVLDKKGSMSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYLEFSKKKEFKINKEWKVTEEQD 292

[Close]

BLAST of Nucleobindin 2 vs. Planmine SMEST
Match: SMESG000013792.1 (SMESG000013792.1)

HSP 1 Score: 146.747 bits (369), Expect = 4.685e-40
Identity = 111/336 (33.04%), Postives = 188/336 (55.95%), Query Frame = 1

Query:   85 CYCTFILY-FAVLSTFCTSSSSEQIEKFIDELNQKEYSEYMRNVMQELLKDKNIQGKIFNQNSTSNKDFMXXXXX---XXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSST-KLSKNERDEMADNLTSYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEESDQ 1077
            CY    L+ F+++S       SE+ E     L + EY  Y++ V+  L  D   + KI  ++  ++ D M  ++     S   R+KLDEIK  E+NRLN ++ +L+++ KSS   LS+ ++D            H++   D KF ++D+ +L+S+       +D   EE+YRRY+ +K+ + +  L K K    + K+      S K ++ H  I+ PG+E+Q KE+W+  D LD + FN  TFF LHD N+DG+ DI EIEA++Q ++ K+YE  N   D  E ++E+++MR+ + ++ D + D +I+  E I +T    FK++ +WK  +E +Q
Sbjct:    5 CYIFISLFVFSIMSAPVIKKPSEEDEIKEGTLPRVEYGNYLKQVIDVLETDPEFKKKI--ESLGNDSDIMSVVNHFDLVSHHVRTKLDEIKRIELNRLNKMEARLEKLQKSSNFSLSRIDKD------------HINIHNDLKFEEEDLRRLISKTVEIANKLDAKAEEEYRRYQMEKEIKRKLRLSKIKSKEMRDKQRLLDAASEKRHKSHPAIHYPGTEEQLKEVWEKEDKLDPNSFNPVTFFRLHDTNEDGHLDIAEIEAIMQIELNKVYEVNNSDDDIVEMYNEMLRMRKEVFKEADTNNDRMISFPEFIRWTKSDKFKTDEDWKIQDELNQ 326

[Close]

BLAST of Nucleobindin 2 vs. Planmine SMEST
Match: SMESG000000846.1 (SMESG000000846.1)

HSP 1 Score: 145.591 bits (366), Expect = 3.279e-39
Identity = 113/340 (33.24%), Postives = 190/340 (55.88%), Query Frame = 1

Query:   61 FFFI*AFMCYCTFILYFAVLSTFCTSSSSEQIEKFIDELNQKEYSEYMRNVMQELLKDKNIQGKIFNQNSTSNKDFMXXXXX-XXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEESDQ 1077
            F  I   + +  + +Y A +      S    +       N   Y EY+  +++EL  DKN   +I +  + +  + +           RS+LDEIK REI RLN L+ +++R    +        D    ++ S+ + HL       F   D+EKL+ ++  DL  +D  +E+Q++ +E +K+   +K+LE  KDP ++ K  E++K+S   ++ H +++ PGSE+Q KE+W   D+LD   FN K FF+LHD+N DG+ DINEIE+++Q ++ K+YE  N   D +EK+HE+M+MR  +M+ +D +KD+L++  E + Y+   +FK + E KT EE  Q
Sbjct:    2 FSIIKILLLFFIYNIYAAPVRKGEVKSEESNL------FNDLHYKEYLDEIIKELESDKNFAERIKDAQAHNRTEHIAQSLQFVHHRIRSRLDEIKRREIERLNVLENEIERRRLEAKGYKVPPPDPKLKSVISFDKEHLDHNNVHTFEMSDLEKLIKKVEDDLSQIDKEQEKQFQSHEMKKEHLKKKKLETIKDPNERKKVEEQMKESEIRHKNHSKVHRPGSEEQLKEVWDKNDHLDPKEFNPKAFFDLHDVNGDGHLDINEIESLMQNELSKVYEANNSDDDMQEKYHEMMRMRLEMMKVIDKNKDNLVSKDEFLQYSQTTNFKEDPELKTQEEDGQ 335

[Close]

BLAST of Nucleobindin 2 vs. Planmine SMEST
Match: SMESG000022270.1 (SMESG000022270.1)

HSP 1 Score: 143.665 bits (361), Expect = 1.049e-38
Identity = 114/337 (33.83%), Postives = 184/337 (54.60%), Query Frame = 1

Query:   79 FMCYCTFILYFAVLSTFCTSSSSEQIEKFIDELNQKEYSEYMRNVMQELLKDKNIQGKIFNQNSTSNKDFMXXXXXXXXXXXXXXXEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMA-DNLTSYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEK-FKDPVQKSKRLEE-----IXXXXXXXXXXXXINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDKDDLITLQELIVYTARKDFKSNIEWKTSEE 1068
            F+  C +I +F            E  E+F   LN  +Y+ Y+  VM EL KD+     I    + S+    +AL++ S + R+KLDE+K  EI RLN L+ +      S  K +K    EM    + S   +HL       F+ +D+++L+ +       +D   +E Y+ YE +K+ + R ELE+ + D    +K LE+     +++ +  + KHK INEPGS +Q K +W++ D +    FN KTFF LHD+N DGN D+ E+E ++Q +++K Y+  +   D  E++HE+ +MR+  +E VD DK+ LI+L+E + +T   +   N EWKT EE
Sbjct:    7 FLLVCLYIDFFQCPPVQPAKKLEESDEEFNKLLNSVDYASYLSEVMSELHKDEKFSKTI--NTAKSSDSVSEALNIVSPNIRTKLDELKRLEIIRLNKLEKE-----ASKRKSNKYHPHEMPIPPIKSVDISHLDDINPESFSPEDLKRLIDRTTEIFNQIDKRNDENYKMYEMEKEAKKRAELERTYSD----TKILEDAQNKWLEEQKSKHSKHKMINEPGSIEQLKSVWQNEDQMSEDSFNPKTFFYLHDVNGDGNLDVAELETLMQKELDKAYDPNDNATDVIERYHEMSRMRKKFLEHVDLDKNHLISLEEFMQWTKSGEAVKNNEWKTQEE 332

The following BLAST results are available for this feature:

BLAST of Nucleobindin 2 vs. Ensembl Human
BLAST of Nucleobindin 2 vs. Ensembl Celegans
BLAST of Nucleobindin 2 vs. Ensembl Fly
BLAST of Nucleobindin 2 vs. Ensembl Zebrafish
BLAST of Nucleobindin 2 vs. Ensembl Xenopus
BLAST of Nucleobindin 2 vs. Ensembl Mouse
BLAST of Nucleobindin 2 vs. UniProt/SwissProt
BLAST of Nucleobindin 2 vs. TrEMBL
BLAST of Nucleobindin 2 vs. Ensembl Cavefish
BLAST of Nucleobindin 2 vs. Ensembl Sea Lamprey
BLAST of Nucleobindin 2 vs. Ensembl Yeast
BLAST of Nucleobindin 2 vs. Ensembl Nematostella
BLAST of Nucleobindin 2 vs. Ensembl Medaka
BLAST of Nucleobindin 2 vs. Planmine SMEST

BLAST of Nucleobindin 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
NUCB1	2.309e-30	34.82	nucleobindin 1 [Source:HGNC Symbol;Acc:HGNC:8043]	[more]
NUCB1	2.309e-30	34.82	nucleobindin 1 [Source:HGNC Symbol;Acc:HGNC:8043]	[more]
NUCB1	2.035e-28	37.57	nucleobindin 1 [Source:HGNC Symbol;Acc:HGNC:8043]	[more]
NUCB2	1.168e-26	30.72	nucleobindin 2 [Source:HGNC Symbol;Acc:HGNC:8044]	[more]
NUCB2	1.448e-26	30.72	nucleobindin 2 [Source:HGNC Symbol;Acc:HGNC:8044]	[more]

BLAST of Nucleobindin 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3


Match Name	E-value	Identity	Description
nucb-1	9.605e-25	34.44	NUCleoBindin homolog [Source:UniProtKB/TrEMBL;Acc...	[more]
nucb-1	1.454e-24	34.44	NUCleoBindin homolog [Source:UniProtKB/TrEMBL;Acc...	[more]
nucb-1	1.454e-24	34.44	NUCleoBindin homolog [Source:UniProtKB/TrEMBL;Acc...	[more]

BLAST of Nucleobindin 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1


Match Name	E-value	Identity	Description
NUCB1	1.010e-20	29.01	gene:FBgn0052190 transcript:FBtr0075171	[more]

BLAST of Nucleobindin 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
nucb2b	3.511e-32	32.54	nucleobindin 2b [Source:ZFIN;Acc:ZDB-GENE-030131-4...	[more]
nucb2b	3.511e-32	32.54	nucleobindin 2b [Source:ZFIN;Acc:ZDB-GENE-030131-4...	[more]
nucb1	5.919e-30	32.76	nucleobindin 1 [Source:ZFIN;Acc:ZDB-GENE-060825-22...	[more]
nucb1	5.919e-30	32.76	nucleobindin 1 [Source:ZFIN;Acc:ZDB-GENE-060825-22...	[more]
nucb2a	7.568e-29	33.00	nucleobindin 2a [Source:ZFIN;Acc:ZDB-GENE-030826-1...	[more]

BLAST of Nucleobindin 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
nucb1	5.778e-29	33.87	nucleobindin 1 [Source:NCBI gene;Acc:407884]	[more]
nucb1	6.245e-29	33.44	nucleobindin 1 [Source:NCBI gene;Acc:407884]	[more]
nucb1	9.418e-29	33.87	nucleobindin 1 [Source:NCBI gene;Acc:407884]	[more]
nucb1	9.418e-29	33.87	nucleobindin 1 [Source:NCBI gene;Acc:407884]	[more]
nucb1	9.418e-29	33.87	nucleobindin 1 [Source:NCBI gene;Acc:407884]	[more]

BLAST of Nucleobindin 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Nucb1	6.850e-32	34.82	nucleobindin 1 [Source:MGI Symbol;Acc:MGI:97388]	[more]
Nucb1	7.195e-32	34.82	nucleobindin 1 [Source:MGI Symbol;Acc:MGI:97388]	[more]
Nucb1	2.884e-29	38.98	nucleobindin 1 [Source:MGI Symbol;Acc:MGI:97388]	[more]
Nucb2	1.265e-27	33.20	nucleobindin 2 [Source:MGI Symbol;Acc:MGI:1858179]	[more]
Nucb2	1.368e-27	33.20	nucleobindin 2 [Source:MGI Symbol;Acc:MGI:1858179]	[more]

BLAST of Nucleobindin 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q63083\|NUCB1_RAT	3.778e-31	34.69	Nucleobindin-1 OS=Rattus norvegicus OX=10116 GN=Nu...	[more]
sp\|Q02819\|NUCB1_MOUSE	4.791e-31	34.82	Nucleobindin-1 OS=Mus musculus OX=10090 GN=Nucb1 P...	[more]
sp\|Q5R4U1\|NUCB1_PONAB	6.287e-30	35.22	Nucleobindin-1 OS=Pongo abelii OX=9601 GN=NUCB1 PE...	[more]
sp\|Q02818\|NUCB1_HUMAN	1.109e-29	34.82	Nucleobindin-1 OS=Homo sapiens OX=9606 GN=NUCB1 PE...	[more]
sp\|Q0P569\|NUCB1_BOVIN	1.470e-29	34.71	Nucleobindin-1 OS=Bos taurus OX=9913 GN=NUCB1 PE=1...	[more]

BLAST of Nucleobindin 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A267H5F8	1.507e-37	33.82	Uncharacterized protein (Fragment) OS=Macrostomum ...	[more]
B7Q350	2.628e-37	32.09	Niemann-Pick type C1 domain-containing protein, pu...	[more]
A0A267FYX5	3.737e-37	33.82	Uncharacterized protein (Fragment) OS=Macrostomum ...	[more]
A0A1I8HPU3	1.999e-35	32.19	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A267DJS5	5.560e-35	31.53	Uncharacterized protein (Fragment) OS=Macrostomum ...	[more]

BLAST of Nucleobindin 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
nucb1	4.832e-28	34.13	nucleobindin-2-like [Source:NCBI gene;Acc:10304653...	[more]
nucb1	1.413e-21	33.10	nucleobindin-2-like [Source:NCBI gene;Acc:10304653...	[more]
nucb2a	8.976e-6	24.43	nucleobindin 2a [Source:ZFIN;Acc:ZDB-GENE-030826-1...	[more]

BLAST of Nucleobindin 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
nucb2b	2.641e-12	28.11	nucleobindin 2b [Source:ZFIN;Acc:ZDB-GENE-030131-4...	[more]

BLAST of Nucleobindin 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Nucleobindin 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO43098	2.736e-23	26.42	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Nucleobindin 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3


Match Name	E-value	Identity	Description
nucb2a	3.432e-31	33.67	nucleobindin-2 [Source:NCBI gene;Acc:101163155]	[more]
nucb1	3.157e-29	34.47	nucleobindin-2 [Source:NCBI gene;Acc:101169036]	[more]
NUCB2	3.719e-29	33.22	nucleobindin 2 [Source:NCBI gene;Acc:101162363]	[more]

BLAST of Nucleobindin 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000016720.1	0.000e+0	99.17	SMESG000016720.1	[more]
SMESG000017142.1	5.583e-99	57.53	SMESG000017142.1	[more]
SMESG000013792.1	4.685e-40	33.04	SMESG000013792.1	[more]
SMESG000000846.1	3.279e-39	33.24	SMESG000000846.1	[more]
SMESG000022270.1	1.049e-38	33.83	SMESG000022270.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30002242 ID=SMED30002242|Name=Nucleobindin 2|organism=Schmidtea mediterranea sexual|type=transcript|length=1242bp
ATTTCTTAGTCGAAGATTACGATTTAAATTTAATCCTACACATTAACTGC
AACACAGAATTTTTTTTTTATCTGAGCATTTATGTGTTACTGTACTTTTA
TACTCTACTTTGCGGTGCTCTCTACATTTTGCACTTCATCTTCATCTGAA
CAAATTGAAAAATTTATTGATGAGTTAAACCAAAAAGAGTATTCGGAATA
TATGCGTAATGTTATGCAAGAACTTTTAAAAGACAAAAATATTCAAGGGA
AAATTTTCAACCAAAATTCTACGTCAAACAAAGATTTTATGGATGCTTTG
GATTTGGCTAGCAGTTCTTCTCGAAGTAAACTCGACGAAATCAAAAGTAG
AGAAATAAATAGATTAAATTCCCTTAAATTACAACTAAAAAGAATGCTTA
AATCAAGCACTAAACTTAGTAAAAACGAAAGAGATGAGATGGCTGACAAT
TTGACTTCTTATAGAAGAACCCATCTGCATTCAACTGAGGATTCCAAATT
CACAAAGGACGACATGGAAAAATTAATGTCTCAGATACGGCATGATTTAC
GATTAGTTGACACTGTTAGAGAGGAACAGTATCGTAGATATGAGTGGCAA
AAAGATTGGAGATTGAGGAAGGAGCTAGAAAAATTCAAAGATCCTGTTCA
GAAGAGTAAACGATTAGAAGAAATTAAAAAGTCAAGAAAATCGTATCGAA
AACATAAACGCATAAATGAACCGGGAAGTGAGGATCAATTTAAAGAGATA
TGGAAATCTGTGGACAATCTCGACATCTCTTATTTTAATATCAAAACCTT
CTTCAATTTGCATGATTTAAATCAAGATGGAAATTGGGATATCAATGAAA
TTGAGGCAGTGGTTCAACCAGACATTGAGAAAATATACGAAGATGCTAAT
GGAACCGCCGATCCAGAAGAAAAGTTTCACGAAATAATGAAAATGAGGCA
GCATTTGATGGAAAAAGTTGACAATGATAAAGATGATTTGATAACGCTGC
AAGAACTGATTGTCTATACGGCAAGGAAAGATTTTAAGAGCAACATCGAA
TGGAAAACCAGTGAAGAAAGTGATCAGTCAATTTATTCAGAGGAAGAAAA
GATAAAATTTGAAAAAGAATTAGAGGAAATTTCTCAATCCAGTGTGGTTA
ATGTGGATGAGTCGCTAAATTGAAATGGTGAGTCGCATTCATTTGAGAGG
CGCAGTATTTTATGTATGATACATGTTTTGGTTAACATCTGC

protein sequence of SMED30002242-orf-1

>SMED30002242-orf-1 ID=SMED30002242-orf-1|Name=SMED30002242-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=364bp
MCYCTFILYFAVLSTFCTSSSSEQIEKFIDELNQKEYSEYMRNVMQELLK
DKNIQGKIFNQNSTSNKDFMDALDLASSSSRSKLDEIKSREINRLNSLKL
QLKRMLKSSTKLSKNERDEMADNLTSYRRTHLHSTEDSKFTKDDMEKLMS
QIRHDLRLVDTVREEQYRRYEWQKDWRLRKELEKFKDPVQKSKRLEEIKK
SRKSYRKHKRINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDG
NWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKVDNDK
DDLITLQELIVYTARKDFKSNIEWKTSEESDQSIYSEEEKIKFEKELEEI
SQSSVVNVDESLN*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **biological process**
Term	Definition
GO:0032099	negative regulation of appetite
GO:0032094	response to food

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut
PLANA:0000028	Category 2 cell
PLANA:0002032	epidermal cell
PLANA:0002075	pigment cell
PLANA:0002109	X1 cell
PLANA:0002111	X2 cell
PLANA:0002142	parenchyma

Vocabulary: **INTERPRO**
Term	Definition
IPR040250	Nucleobindin
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **molecular function**
Term	Definition
GO:0005509	calcium ion binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 216..318 e-value: 1.4E-22 score: 81.8
None	No IPR available	SIGNALP_EUK	SignalP-noTM	SignalP-noTM	coord: 1..22 score: 0.752
IPR040250	Nucleobindin	PANTHER	PTHR19237	NUCLEOBINDIN	coord: 32..349
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 297..309
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 220..314

Main menu

Nucleobindin 2

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 12

Owlery

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