SMED30002222

Overview


Name	SMED30002222
Smed ID	SMED30002222
Length (bp)	770

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30002222

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Expressed In	Reference Transcript	Published Transcript	Transcriptome	Publication
pharynx	SMED30002222	Contig7536	newmark_ests	PMID:23079596 Forsthoefel et al., 2012
pharynx	SMED30002222	Contig7536	uc_Smed_v2	PMID:23079596 Forsthoefel et al., 2012
gut	SMED30002222	Contig7536	newmark_ests	PMID:23079596 Forsthoefel et al., 2012
gut	SMED30002222	Contig7536	uc_Smed_v2	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30002222	Contig7536	newmark_ests	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30002222	Contig7536	uc_Smed_v2	PMID:23079596 Forsthoefel et al., 2012

Note: Hover over icons to view figure legend

Homology

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BLAST of SMED30002222 vs. Ensembl Human
Match: ZBED9 (zinc finger BED-type containing 9 [Source:HGNC Symbol;Acc:HGNC:13851])

HSP 1 Score: 70.8626 bits (172), Expect = 2.532e-13
Identity = 47/152 (30.92%), Postives = 84/152 (55.26%), Query Frame = -3

Query:  127 AVSDYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFSTNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGE-FIKECLENVA-DIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 576
            A+    LKRH + KH          + + K +ELKS     +FN + + N SAL+ASY VA  +AK   P+T  E  +K+C++ V  +++       ++++ LS+ +IARRI+++   +E +L     +  Y+SL +DE  D+++   + +Y
Sbjct:  619 AMKPSKLKRHLYSKHKEISSQPKEFFER-KSSELKSQ-PKQVFN-VSHINISALRASYKVALPVAKSKTPYTIAETLVKDCIKEVCLEMLGESAAKKVAQVPLSNDTIARRIQELANDMEDQLIEQIKLAKYFSLQLDECRDIANMIILLVY 767

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BLAST of SMED30002222 vs. Ensembl Human
Match: ZBED9 (zinc finger BED-type containing 9 [Source:HGNC Symbol;Acc:HGNC:13851])

HSP 1 Score: 69.707 bits (169), Expect = 7.761e-13
Identity = 47/152 (30.92%), Postives = 84/152 (55.26%), Query Frame = -3

Query:  127 AVSDYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFSTNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGE-FIKECLENVA-DIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 576
            A+    LKRH + KH          + + K +ELKS     +FN + + N SAL+ASY VA  +AK   P+T  E  +K+C++ V  +++       ++++ LS+ +IARRI+++   +E +L     +  Y+SL +DE  D+++   + +Y
Sbjct:  770 AMKPSKLKRHLYSKHKEISSQPKEFFER-KSSELKSQ-PKQVFN-VSHINISALRASYKVALPVAKSKTPYTIAETLVKDCIKEVCLEMLGESAAKKVAQVPLSNDTIARRIQELANDMEDQLIEQIKLAKYFSLQLDECRDIANMIILLVY 918

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BLAST of SMED30002222 vs. Ensembl Human
Match: ZBED5 (zinc finger BED-type containing 5 [Source:HGNC Symbol;Acc:HGNC:30803])

HSP 1 Score: 50.447 bits (119), Expect = 1.344e-6
Identity = 37/107 (34.58%), Postives = 61/107 (57.01%), Query Frame = -3

Query:  127 IDNQNDSALKASYVVANIIAKKSKPFTDGEF-IKECLENVADIICPDTQSY-ISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 441
            ++  N+SA +ASY V+  IA   +  T GE  IK C ++V   +  +  S  I  ++LS+ ++ARRI+D+   IE  L     I + +SL +DES D+S  A + ++
Sbjct:  186 VNTDNESATEASYNVSYHIALSGEAHTIGELLIKPCAKDVVMRMFDEQYSKKIDAVQLSNSTVARRIKDLAADIEEELVCRLKICDGFSLQLDESADVSGLAVLLVF 292

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Query:  127 IDNQNDSALKASYVVANIIAKKSKPFTDGEF-IKECLENVADIICPDTQSY-ISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 441
            ++  N+SA +ASY V+  IA   +  T GE  IK C ++V   +  +  S  I  ++LS+ ++ARRI+D+   IE  L     I + +SL +DES D+S  A + ++
Sbjct:  186 VNTDNESATEASYNVSYHIALSGEAHTIGELLIKPCAKDVVMRMFDEQYSKKIDAVQLSNSTVARRIKDLAADIEEELVCRLKICDGFSLQLDESADVSGLAVLLVF 292

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BLAST of SMED30002222 vs. Ensembl Human
Match: EPM2AIP1 (EPM2A interacting protein 1 [Source:HGNC Symbol;Acc:HGNC:19735])

HSP 1 Score: 50.447 bits (119), Expect = 1.366e-6
Identity = 38/165 (23.03%), Postives = 79/165 (47.88%), Query Frame = -3

Query:   85 LAVSDYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFSTNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALYNITQVLATNINTDL 579
            +A  + +++RHY  +H   Y+ Y  +   ++ A ++ +   ++        + A +A   +  ++A K + + +G+F+ +C+E +   + P+  S +  + LS     +RI  I +++  +L N +  F  YSLA+D      D A VA  N   V    +  +L
Sbjct:   47 VATRERDVRRHYEAEH-EYYERY--VADGERAALVERLRQGDLPVASFTPEERAARAGLGLCRLLALKGRGWGEGDFVYQCMEVLLREVLPEHVSVLQGVDLSPDITRQRILSIDRNLRNQLFNRARDFKAYSLALD------DQAFVAYENYLLVFIRGVGPEL 202

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BLAST of SMED30002222 vs. Ensembl Zebrafish
Match: AL928808.1 (pep chromosome:GRCz11:20:17257101:17259573:-1 gene:ENSDARG00000101333.2 transcript:ENSDART00000166397.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:AL928808.1)

HSP 1 Score: 89.3521 bits (220), Expect = 7.608e-20
Identity = 49/152 (32.24%), Postives = 83/152 (54.61%), Query Frame = -3

Query:  127 NLKRHYFQKHVSTYDAY--NGMYSKDKVAELKSVFS--TNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSY---ISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 561
            NL+RH+   H +    +       K KV  LK      T +F +    +++   ASY +A  IA+  KP+ +GEF+K+CL +   I+CP+ ++    +  ++LS  ++ +RI DI  S+ET L +      Y+S+A+DES D+ D  Q A++
Sbjct:   53 NLERHFTSLHSAVAREFPKGSELRKHKVKTLKGQAEKQTQLFRKFSKHSETVTLASYQLAWNIARAKKPYLEGEFVKKCLSDAVAILCPENENLKRSVKDLQLSRHTVEQRISDIDNSVETHLLSDLQKCQYFSIALDESCDVQDKPQFAIF 204

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BLAST of SMED30002222 vs. Ensembl Zebrafish
Match: CR392001.3 (pep chromosome:GRCz11:8:38963323:38965260:-1 gene:ENSDARG00000117159.1 transcript:ENSDART00000181495.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR392001.3)

HSP 1 Score: 48.1358 bits (113), Expect = 5.738e-6
Identity = 53/187 (28.34%), Postives = 85/187 (45.45%), Query Frame = -3

Query:   55 NLKRHYFQKHVSTYDAYN-GMYSKDKVAEL--KSVFSTNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQ---SYISKIR---LSHQSIARR-IEDIGKSIETRLGNGSVIFNYYSLAIDESTDL--------SDTAQVALYNITQVLATNINTDLYENVNKILFE 561
            N++RH+  +H +    Y  G   K  +A L  K     N F +     +S   AS+V    I K+ KPFTDG+++KE   N+++ +  D +     I KI+   LS +++  R I+  G   E ++ + +     YS+A DES D         SD  Q  +  +  +       D+ E V K L E
Sbjct:   51 NVERHFQGRHATFAAEYPVGSERKSAIALLVEKLEERKNKFKKWIASPNSTTAASFVATREIIKRGKPFTDGDYMKESFINISEHLFSDFKNKTEIIQKIKDMPLSAKTVKERAIKMAGNITEQQIKDINSA-PAYSIACDESCDTALLCRYVNSDGPQEEIIKLIPLKGQTRGEDICEAVLKCLNE 236

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BLAST of SMED30002222 vs. Ensembl Xenopus
Match: spag1 (sperm associated antigen 1 [Source:Xenbase;Acc:XB-GENE-853609])

HSP 1 Score: 97.0561 bits (240), Expect = 2.527e-22
Identity = 54/153 (35.29%), Postives = 87/153 (56.86%), Query Frame = -3

Query:  127 AVSDYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFST--NIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQS-YISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 576
            +  +YN+KRHY   H + Y  Y G      + +LKS      NIF+ +     +A+KAS++VA  IAK+  PF+ G  +K C   +A+    +T +  +  I LSHQ++AR I  I + +  +L N      Y+S+A+DESTD++DT Q+ ++
Sbjct:   55 SCKEYNVKRHYTTLHGAKYAKYTGEARLTIIKDLKSKLCKQKNIFSAVTTTQKAAVKASFLVAEEIAKRKYPFSSGTLVKTCALKMAEAFGNETMAKTVESISLSHQTVARHICTINQHLTDKLQNILRSAPYFSIALDESTDITDTCQLVIF 207

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BLAST of SMED30002222 vs. Ensembl Xenopus
Match: ENSXETT00000016563.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:4:37379994:37381838:-1 gene:ENSXETG00000009412.1 transcript:ENSXETT00000016563.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 97.0561 bits (240), Expect = 2.771e-22
Identity = 54/153 (35.29%), Postives = 87/153 (56.86%), Query Frame = -3

Query:  127 AVSDYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFST--NIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQS-YISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 576
            +  +YN+KRHY   H + Y  Y G      + +LKS      NIF+ +     +A+KAS++VA  IAK+  PF+ G  +K C   +A+    +T +  +  I LSHQ++AR I  I + +  +L N      Y+S+A+DESTD++DT Q+ ++
Sbjct:   55 SCKEYNVKRHYTTLHGAKYAKYTGEARLTIIKDLKSKLCKQKNIFSAVTTTQKAAVKASFLVAEEIAKRKYPFSSGTLVKTCALKMAEAFGNETMAKTVESISLSHQTVARHICTINQHLTDKLQNILRSAPYFSIALDESTDITDTCQLVIF 207

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BLAST of SMED30002222 vs. Ensembl Xenopus
Match: ENSXETT00000028445.1 (general transcription factor II-I repeat domain-containing protein 2-like [Source:NCBI gene;Acc:101734469])

HSP 1 Score: 69.707 bits (169), Expect = 5.574e-13
Identity = 48/155 (30.97%), Postives = 79/155 (50.97%), Query Frame = -3

Query:  127 LAVS-DYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFSTN-IFNRIDNQNDS-ALKASYVVANIIAKKSKPFTDGEFIKECLENVADIIC-PDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 579
            LAV  +YN+KRHY   H  TY  Y G +    + EL+S  +       I+   D+ ALKAS+++   I K+ + F   + +KEC   +A +    +  +    I L  Q++ARR+  + + I  +L        Y+SLA+DE+TD   T  + ++
Sbjct:   42 LAVCKEYNVKRHYISLHEKTYSQYTGDHRMAILNELRSKLNKQETVTSIETATDTAALKASFIICEEIVKRKQAFDSVDVVKECAVKMARVFGNEEMAAKFDSISLQPQTVARRVSAMHQQITQKLHAILKNCCYFSLALDETTDSCGTDLLVIF 196

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BLAST of SMED30002222 vs. Ensembl Xenopus
Match: ENSXETT00000010836.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:7:63865670:63866962:-1 gene:ENSXETG00000001503.1 transcript:ENSXETT00000010836.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 49.2914 bits (116), Expect = 1.238e-6
Identity = 26/56 (46.43%), Postives = 38/56 (67.86%), Query Frame = -3

Query:  334 KDKVAELKSVFSTN--IFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECL 495
            K KV ELKSV S    IF +  N++ +A  AS+ VA+++AKK KP+ DGE +K+ +
Sbjct:   62 KVKVKELKSVLSAQQAIFKKPANKSKAATIASFKVAHVLAKKKKPYEDGEIVKDAM 117

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BLAST of SMED30002222 vs. Ensembl Mouse
Match: Epm2aip1 (EPM2A (laforin) interacting protein 1 [Source:MGI Symbol;Acc:MGI:1925031])

HSP 1 Score: 53.5286 bits (127), Expect = 1.001e-7
Identity = 39/160 (24.38%), Postives = 77/160 (48.12%), Query Frame = -3

Query:   85 NLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFSTNI-FNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALYNITQVLATNINTDL 561
            +++RHY  +H   Y+ + G   +++ A ++ +   ++    +    + A +A   +   +A K + + +G+F+ +C+E +   + PD    +  I LS +   +RI  I  ++ ++L N +  F  YSLA+D      D A VA  N   V    +  DL
Sbjct:   53 DVRRHYEAEH-EFYERFVG--DEERAALVERLRQGDMSLAAVLTPEERATRAGLGLCRFLALKGRGWGEGDFVHQCMEVLLREVLPDHVGVLEGIDLSPEITRQRILSIDSNLRSQLFNRARDFKAYSLALD------DQAFVAYENYLLVFIRGVGRDL 203

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Query:   85 NLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFSTNI-FNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALYNITQVLATNINTDL 561
            +++RHY  +H   Y+ + G   +++ A ++ +   ++    +    + A +A   +   +A K + + +G+F+ +C+E +   + PD    +  I LS +   +RI  I  ++ ++L N +  F  YSLA+D      D A VA  N   V    +  DL
Sbjct:   53 DVRRHYEAEH-EFYERFVG--DEERAALVERLRQGDMSLAAVLTPEERATRAGLGLCRFLALKGRGWGEGDFVHQCMEVLLREVLPDHVGVLEGIDLSPEITRQRILSIDSNLRSQLFNRARDFKAYSLALD------DQAFVAYENYLLVFIRGVGRDL 203

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BLAST of SMED30002222 vs. Ensembl Mouse
Match: Zbed5 (zinc finger, BED type containing 5 [Source:MGI Symbol;Acc:MGI:1919220])

HSP 1 Score: 51.6026 bits (122), Expect = 4.986e-7
Identity = 43/148 (29.05%), Postives = 77/148 (52.03%), Query Frame = -3

Query:  127 LKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFSTNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFI--KECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGN--GSVIFNYYSLAIDESTDLSDTAQVALY 558
            LKRH+  KH S++   +  Y + K   LK     +  ++ + QN +A++ SY+VA  IA+  KP T  E +      + V  +I  +  + +S + LS+ ++ RRI D+   I  ++     S      S+ +DESTD+++  Q+ +Y
Sbjct:  208 LKRHFESKH-SSFAGKDTNYFRSKADGLKKA-RPDTGSKSNKQNVAAVETSYLVALRIARDMKPHTFAEHLLFPVAKDRVRVMIGDEFVTKLSAVSLSNDTVRRRIHDMSADILDQVVQEIKSAPLPICSIQLDESTDVANCLQLMVY 353

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BLAST of SMED30002222 vs. UniProt/SwissProt
Match: sp|Q6R2W3|SCND3_HUMAN (SCAN domain-containing protein 3 OS=Homo sapiens OX=9606 GN=ZBED9 PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 3.727e-12
Identity = 47/152 (30.92%), Postives = 84/152 (55.26%), Query Frame = -3

Query:  127 AVSDYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFSTNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGE-FIKECLENVA-DIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 576
            A+    LKRH + KH          + + K +ELKS     +FN + + N SAL+ASY VA  +AK   P+T  E  +K+C++ V  +++       ++++ LS+ +IARRI+++   +E +L     +  Y+SL +DE  D+++   + +Y
Sbjct:  770 AMKPSKLKRHLYSKHKEISSQPKEFFER-KSSELKSQ-PKQVFN-VSHINISALRASYKVALPVAKSKTPYTIAETLVKDCIKEVCLEMLGESAAKKVAQVPLSNDTIARRIQELANDMEDQLIEQIKLAKYFSLQLDECRDIANMIILLVY 918

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BLAST of SMED30002222 vs. UniProt/SwissProt
Match: sp|A4IFA3|GT2D2_BOVIN (General transcription factor II-I repeat domain-containing protein 2 OS=Bos taurus OX=9913 GN=GTF2IRD2 PE=2 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 2.431e-7
Identity = 35/147 (23.81%), Postives = 62/147 (42.18%), Query Frame = -3

Query:  127 DYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFSTNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 567
            +YNL+RHY   H   YD Y      +K+ ELK                                       E +++ L   +D +CP+ +   +K+     +  + +ED+  ++  +L      F  YS+AIDE TD+++T Q+A++
Sbjct:  460 EYNLRRHYQTNHSKHYDQYTEKMRDEKLQELK---------------------------------------EGLRKYLLGSSDTVCPEQKQVFAKVNPRENAAVQPVEDVAGNLWEKLREKIRSFVAYSIAIDEITDINNTTQLAIF 567

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BLAST of SMED30002222 vs. UniProt/SwissProt
Match: sp|Q8VEH5|EPMIP_MOUSE (EPM2A-interacting protein 1 OS=Mus musculus OX=10090 GN=Epm2aip1 PE=1 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 7.002e-7
Identity = 39/160 (24.38%), Postives = 77/160 (48.12%), Query Frame = -3

Query:   85 NLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFSTNI-FNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALYNITQVLATNINTDL 561
            +++RHY  +H   Y+ + G   +++ A ++ +   ++    +    + A +A   +   +A K + + +G+F+ +C+E +   + PD    +  I LS +   +RI  I  ++ ++L N +  F  YSLA+D      D A VA  N   V    +  DL
Sbjct:   53 DVRRHYEAEH-EFYERFVG--DEERAALVERLRQGDMSLAAVLTPEERATRAGLGLCRFLALKGRGWGEGDFVHQCMEVLLREVLPDHVGVLEGIDLSPEITRQRILSIDSNLRSQLFNRARDFKAYSLALD------DQAFVAYENYLLVFIRGVGRDL 203

[Close]

BLAST of SMED30002222 vs. UniProt/SwissProt
Match: sp|Q49AG3|ZBED5_HUMAN (Zinc finger BED domain-containing protein 5 OS=Homo sapiens OX=9606 GN=ZBED5 PE=2 SV=2)

HSP 1 Score: 50.447 bits (119), Expect = 6.454e-6
Identity = 37/107 (34.58%), Postives = 61/107 (57.01%), Query Frame = -3

Query:  127 IDNQNDSALKASYVVANIIAKKSKPFTDGEF-IKECLENVADIICPDTQSY-ISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 441
            ++  N+SA +ASY V+  IA   +  T GE  IK C ++V   +  +  S  I  ++LS+ ++ARRI+D+   IE  L     I + +SL +DES D+S  A + ++
Sbjct:  186 VNTDNESATEASYNVSYHIALSGEAHTIGELLIKPCAKDVVMRMFDEQYSKKIDAVQLSNSTVARRIKDLAADIEEELVCRLKICDGFSLQLDESADVSGLAVLLVF 292

[Close]

BLAST of SMED30002222 vs. UniProt/SwissProt
Match: sp|A4Z943|ZBED5_BOVIN (Zinc finger BED domain-containing protein 5 OS=Bos taurus OX=9913 GN=ZBED5 PE=4 SV=1)

HSP 1 Score: 50.447 bits (119), Expect = 6.516e-6
Identity = 37/107 (34.58%), Postives = 61/107 (57.01%), Query Frame = -3

Query:  127 IDNQNDSALKASYVVANIIAKKSKPFTDGEF-IKECLENVADIICPDTQSY-ISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 441
            ++  N+SA +ASY V+  IA   +  T GE  IK C ++V   +  +  S  I  ++LS+ ++ARRI+D+   IE  L     I + +SL +DES D+S  A + ++
Sbjct:  187 VNTDNESATEASYNVSYHIALSGEAHTIGELLIKPCAKDVVMRMFDEQYSKKIDAVQLSNSTVARRIKDLAADIEEELVCRLKICDGFSLQLDESADVSGLAVLLVF 293

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BLAST of SMED30002222 vs. TrEMBL
Match: J9JJ25 (DUF4371 domain-containing protein OS=Acyrthosiphon pisum OX=7029 GN=103308085 PE=4 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 3.431e-37
Identity = 73/166 (43.98%), Postives = 106/166 (63.86%), Query Frame = -3

Query:  106 VSDYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFS--TNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVAL--------YNITQVLA 573
            + +YNLKRHY  KH + YD   G    DK+A L       ++   +    +++++KAS+++A  I+ KSKPFTDGEFIKEC+E  ++I+CP  +   SK+ LS  ++ARRIE++G  IE+ L      F +YSL +DESTDLSDTAQ+A+        +NIT+ LA
Sbjct:   45 MKEYNLKRHYGTKHAAKYDMIQGQLRIDKLALLMKNIQGQSSGLKKYHKDSEASVKASFIIAQKISAKSKPFTDGEFIKECMEAASEILCPAQKQLFSKLSLSGVTVARRIEELGTDIESTLKERISKFIFYSLTLDESTDLSDTAQLAIFVRGIDSNFNITEELA 210

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BLAST of SMED30002222 vs. TrEMBL
Match: A0A1S4G7B0 (Uncharacterized protein OS=Aedes aegypti OX=7159 PE=4 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 5.155e-36
Identity = 70/166 (42.17%), Postives = 113/166 (68.07%), Query Frame = -3

Query:  106 VSDYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKS--VFSTNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVAL--------YNITQVLA 573
            + ++N+KRH+  KH S ++  +G    DK+ EL+   +   ++F + +++++S +KASYV++  IA+ SK F +GEFIKECL +VA+I+CP+ +    KI LS  ++ RRIE+IG+  E+RL   +  F +YS+A+DESTD+SDTAQ+A+        +NIT+ LA
Sbjct:   49 IKEFNIKRHFDTKHASKFENLSGNLRADKLRELQRQMIHQHSVFKKQNSESESLVKASYVISEKIARSSKSFMEGEFIKECLVSVAEILCPNQKKVFEKISLSAPTVTRRIEEIGEEFESRLKEKAGQFVFYSVALDESTDISDTAQLAIFVRGIDEKFNITEELA 214

[Close]

BLAST of SMED30002222 vs. TrEMBL
Match: A0A0P6J0U1 (Proteinral transcription factor ii-i repeat domain-containing protein 2a (Fragment) OS=Aedes aegypti OX=7159 PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 3.953e-35
Identity = 68/166 (40.96%), Postives = 113/166 (68.07%), Query Frame = -3

Query:  106 VSDYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKS--VFSTNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVAL--------YNITQVLA 573
            + ++N+KRH+  KH S ++ ++G    DK+ +L+   +   ++F + +++++S +KASYV++  IA+  K F +GEFIKECL +VA+I+CP+ +    KI LS  ++ RRIE+IG+  E+RL   +  F +YS+A+DESTD+SDTAQ+A+        +NIT+ LA
Sbjct:   40 IKEFNIKRHFDTKHASKFENHSGNLRADKLRKLQRQMIHQHSVFKKQNSESESLVKASYVISEKIARSPKSFMEGEFIKECLVSVAEILCPNQKKVFEKISLSDPTVTRRIEEIGEEFESRLKEKAGQFVFYSVALDESTDISDTAQLAIFVRGIDEKFNITEELA 205

[Close]

BLAST of SMED30002222 vs. TrEMBL
Match: A0A2T7P2Y2 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_12960 PE=4 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 4.778e-34
Identity = 68/188 (36.17%), Postives = 117/188 (62.23%), Query Frame = -3

Query:   61 VSDYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFS--TNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVAL--------YNITQVLAT-------NINTDLYENVNKIL 573
            V ++N+KRHY  KH + YD   G    +K+A+L+      T IF++   +N++A+ ASY VA+I++++ KPF+DGE +K+CL  V + +CP+ ++    + LS  +IARR+ED+GK ++++L + +  F  +S+A DESTD SDTAQ+ +        + +T+ LA         I  DL++ V+ ++
Sbjct:   56 VHEHNIKRHYETKHKNKYDGILGSSRTEKIAKLRQEIGGLTRIFSKKRQENEAAVSASYRVAHILSREVKPFSDGECVKKCLLAVVEELCPEKKNAFEAVSLSRTTIARRVEDMGKHLQSKLRDKAARFEMFSIAADESTDSSDTAQLLVFIRGTNSDFKLTEELAAMRGMHGNTIGEDLFKEVSAVI 243

[Close]

BLAST of SMED30002222 vs. TrEMBL
Match: A0A3B1J537 (DUF4371 domain-containing protein OS=Astyanax mexicanus OX=7994 PE=4 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 4.613e-33
Identity = 65/149 (43.62%), Postives = 97/149 (65.10%), Query Frame = -3

Query:  127 DYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVF--STNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 567
            +YNLKRHY  +H   Y  Y G    ++VA LK+      + F +   +ND+A++ASYVV+ +IAK  KPFT+GEF+K+C+   A I+CP+ +   S I LS  ++A RI D+   I  +L   +  F+ YS+A+DESTD++DTAQ+A+Y
Sbjct:  102 EYNLKRHYTTRHAEEYAKYQGDERANRVANLKTCLLRQQDFFKKATKENDAAVEASYVVSEMIAKAGKPFTEGEFVKKCILQAASIVCPEKKGQFSNISLSANTVAERISDLSSDIYDQLCEKAKRFSVYSVALDESTDITDTAQLAIY 250

[Close]

BLAST of SMED30002222 vs. Ensembl Cavefish
Match: ENSAMXT00000030525.1 (pep primary_assembly:Astyanax_mexicanus-2.0:1:12992682:12994541:1 gene:ENSAMXG00000035573.1 transcript:ENSAMXT00000030525.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 136.732 bits (343), Expect = 1.747e-36
Identity = 65/149 (43.62%), Postives = 97/149 (65.10%), Query Frame = -3

Query:  127 DYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVF--STNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 567
            +YNLKRHY  +H   Y  Y G    ++VA LK+      + F +   +ND+A++ASYVV+ +IAK  KPFT+GEF+K+C+   A I+CP+ +   S I LS  ++A RI D+   I  +L   +  F+ YS+A+DESTD++DTAQ+A+Y
Sbjct:  102 EYNLKRHYTTRHAEEYAKYQGDERANRVANLKTCLLRQQDFFKKATKENDAAVEASYVVSEMIAKAGKPFTEGEFVKKCILQAASIVCPEKKGQFSNISLSANTVAERISDLSSDIYDQLCEKAKRFSVYSVALDESTDITDTAQLAIY 250

[Close]

BLAST of SMED30002222 vs. Ensembl Cavefish
Match: ENSAMXT00000035253.1 (general transcription factor II-I repeat domain-containing protein 2B-like [Source:NCBI gene;Acc:111191579])

HSP 1 Score: 108.997 bits (271), Expect = 9.428e-27
Identity = 55/152 (36.18%), Postives = 97/152 (63.82%), Query Frame = -3

Query:  127 VSDYNLKRHYFQKHVSTYDAYNGMYSKDKVA-ELKSVFST--NIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 573
            + +YNL+RH+  KH + Y   + +  K ++A ELK    +  N+F +   +N +A+KAS++VA  IA+ SK F+ G F+K+C+  V + +CPD       + LS  +IA R++++ +++ T+L   +  +  +SLA+DESTD +DTAQ+A++
Sbjct:   43 IKEYNLRRHFDTKHGAKYGKAS-LQEKQQIAQELKDKLKSQQNLFTKATAKNSAAVKASFIVAEEIARASKYFSGGAFLKQCMLKVCEQVCPDQIQTFKNVSLSRNTIADRVKELAENLATQLAEETRSYTAFSLAVDESTDNTDTAQLAIF 193

[Close]

BLAST of SMED30002222 vs. Ensembl Cavefish
Match: ENSAMXT00000052542.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000549.1:35642:40620:1 gene:ENSAMXG00000033785.1 transcript:ENSAMXT00000052542.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 107.842 bits (268), Expect = 2.341e-26
Identity = 56/149 (37.58%), Postives = 88/149 (59.06%), Query Frame = -3

Query:  127 DYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKS--VFSTNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 567
            +YNL+RHY  KH    D   G   K K+  LK   V    +F +   ++++   ASY +AN++AK ++PF DGEF+K+C+    D+ICP+ +     I LS  +I RRI D+  +   +L +      Y+S A+DESTD +DTAQ++++
Sbjct:   48 EYNLRRHYETKHPKLAD-LQGEDRKQKIEALKKQLVSQQGVFEKRRKESEACTIASYRIANLVAKSARPFIDGEFVKDCMLEACDVICPEAKDAFKNISLSRTTIQRRIVDLSSNSYCQLQSRINDVTYFSAALDESTDNTDTAQLSIF 195

[Close]

BLAST of SMED30002222 vs. Ensembl Cavefish
Match: ENSAMXT00000038753.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000657.1:4925:12560:-1 gene:ENSAMXG00000034887.1 transcript:ENSAMXT00000038753.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 107.457 bits (267), Expect = 2.544e-26
Identity = 51/151 (33.77%), Postives = 93/151 (61.59%), Query Frame = -3

Query:  127 VSDYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFST--NIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 573
            + +YNL+RH+  KH + Y   +    +    ELK    +  N+F +   +N +++KAS++VA  IA+ SK F++G F+K+C+  V + +CPD       + LS  +I  R++++ +++ T+L   +  +  +SLA+DESTD +DTAQ+A++
Sbjct:   43 IKEYNLRRHFDTKHGAKYGKASLQEKQQIAQELKDKLKSQQNLFTKATAKNSASVKASFIVAEEIARASKSFSEGAFLKQCMLKVCEQVCPDQIQTFKNVSLSRNTIVDRVKELAENLATQLAEETRSYTAFSLAVDESTDNTDTAQLAIF 193

[Close]

BLAST of SMED30002222 vs. Ensembl Cavefish
Match: ENSAMXT00000037725.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:29162452:29166863:1 gene:ENSAMXG00000037303.1 transcript:ENSAMXT00000037725.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 105.916 bits (263), Expect = 1.449e-25
Identity = 55/149 (36.91%), Postives = 87/149 (58.39%), Query Frame = -3

Query:  127 DYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKS--VFSTNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 567
            +YNL+RHY  KH    D   G   K K+  LK   V    +F +   ++++   ASY +AN++AK ++PF DGEF+K+C+    D+ICP+ +     I LS  +I  RI D+  +   +L +      Y+S A+DESTD +DTAQ++++
Sbjct:  236 EYNLRRHYETKHPKLAD-LQGEDRKQKIEALKKQLVSQQGVFEKRRKESEACTIASYRIANLVAKSARPFIDGEFVKDCMLEACDVICPEAKDAFKNISLSRTTIQLRIVDLSSNSYCQLQSRINDVTYFSAALDESTDNTDTAQLSIF 383

[Close]

BLAST of SMED30002222 vs. Ensembl Medaka
Match: ENSORLT00000027093.1 (general transcription factor II-I repeat domain-containing protein 2B-like [Source:NCBI gene;Acc:111947639])

HSP 1 Score: 125.561 bits (314), Expect = 1.384e-32
Identity = 68/189 (35.98%), Postives = 110/189 (58.20%), Query Frame = -3

Query:   61 DYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFS-----TNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQV--------ALYNITQVLA-------TNINTDLYENVNKIL 567
            DYNL RHY  KH    + Y  +  +++  E +++         +  ++    ++A++ S+V+++ IA+ SKPF+DGEF+KECL +   +ICP+T+S    I LS +++ARRIEDI  ++E +L + +  F+ +SLA+DES D+ DTAQ+        A + IT+ LA       T    DL+  VN  L
Sbjct:   32 DYNLNRHYTTKH---EEKYRNLSDEERAKESEALLQKLQTQQGLLTKLHAPRNAAVRTSFVISHKIARNSKPFSDGEFVKECLLDSVALICPETKSAFENISLSRRTVARRIEDIAGNLELQLKSRAADFDCFSLALDESCDIRDTAQLLIFLRGITAEFQITEELAAMRSMKGTTTGQDLFREVNACL 217

[Close]

BLAST of SMED30002222 vs. Ensembl Medaka
Match: ENSORLT00000033014.1 (general transcription factor II-I repeat domain-containing protein 2 [Source:NCBI gene;Acc:101164918])

HSP 1 Score: 123.635 bits (309), Expect = 8.106e-32
Identity = 67/174 (38.51%), Postives = 107/174 (61.49%), Query Frame = -3

Query:   52 DYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFST--NIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALYNITQVLATNINTDLYENVNKILFEL 567
            ++N++RHY  KH S YD   G+  ++K+ +L+S  +T  +I       +D+  +ASY VA +IAK  KPFTDGEFIK+CL  + + ICP+ +   + + L H ++ +RIEDI  +I+ +L      F ++SLA DESTD S+TAQ+ ++         ++ D+  NV++ L EL
Sbjct:   48 EFNIRRHYQTKHAS-YDNLTGVQRREKLEQLESALTTQQSICAIKSEPDDNTTQASYEVATLIAKHCKPFTDGEFIKDCLMKIVETICPEKKHEFANVCLDHNTVVQRIEDISIAIKRQLEVKGAKFEFFSLACDESTDASNTAQLLIF------LRGVDRDM--NVSEELLEL 212

[Close]

BLAST of SMED30002222 vs. Ensembl Medaka
Match: ENSORLT00000028655.1 (pep primary_assembly:ASM223467v1:8:18412528:18414615:1 gene:ENSORLG00000023148.1 transcript:ENSORLT00000028655.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 112.849 bits (281), Expect = 3.820e-28
Identity = 65/190 (34.21%), Postives = 105/190 (55.26%), Query Frame = -3

Query:   61 VSDYNLKRHY--FQKHVSTYDAYNGMYSKDKVAELKSVFSTN--IFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY--------NITQVL-------ATNINTDLYENVNKIL 573
            + +YNL+RH+   +KH       +      KV ELK    +   +F    +Q+ +A+KAS++VA  + K ++PFT+GEFIK C+  V D +CPD +   S + LS  ++A RI+ +  +++ +L      F  YSLA+DESTD SD AQ++++        +IT+ L        T    DLYE V++ +
Sbjct:   49 MKEYNLRRHHETSKKHTDKDKNMDTEQRLQKVEELKRGLKSQQALFTIAKSQSGAAVKASFIVAEEVEKSARPFTEGEFIKNCMLKVCDAVCPDKRQLFSNVSLSRNTVAERIDQLSTNLKEQLVGKGKDFIAYSLAVDESTDTSDIAQLSIFIRGVDSSLSITEELLALRPLHGTTTGQDLYEEVSRCV 238

[Close]

BLAST of SMED30002222 vs. Ensembl Medaka
Match: ENSORLT00000044825.1 (general transcription factor II-I repeat domain-containing protein 2-like [Source:NCBI gene;Acc:111949247])

HSP 1 Score: 75.8702 bits (185), Expect = 2.242e-15
Identity = 53/186 (28.49%), Postives = 86/186 (46.24%), Query Frame = -3

Query:   61 VSDYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFSTNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY--------NITQVLA-------TNINTDLYENVNKIL 573
            + +YN++RHY  KH + +  Y G   + K +EL         +++ +Q  +                        +  CL   A I+CP  +   S+I LS  ++ RR+ED+ + I  +L   S  F  +SLA DESTD+SD+AQ+ ++         ITQ LA       T    DL+  V+++L
Sbjct:   44 LKEYNIRRHYETKH-AVFSRYKGEARQKKSSEL--------LSKLRSQQAT------------------------LTRCLSTAASIMCPSQERVFSQISLSRNTVTRRVEDLSRDIRDQLTVKSRDFVAFSLACDESTDISDSAQLLVFVRGINADMEITQELAGLETLRGTTKGEDLFAAVSRVL 196

[Close]

BLAST of SMED30002222 vs. Ensembl Medaka
Match: ENSORLT00000038351.1 (pep primary_assembly:ASM223467v1:3:5078117:5081344:-1 gene:ENSORLG00000025675.1 transcript:ENSORLT00000038351.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 73.559 bits (179), Expect = 1.201e-14
Identity = 47/174 (27.01%), Postives = 87/174 (50.00%), Query Frame = -3

Query:   67 LAVSDYNLKRHYFQKHVSTYDAYNGMYSKDKVA-ELKSVFST--NIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALYNITQVLATNINTDLYENVNK 579
            L V +YNL+ H   KH + Y A   +  K ++A ELK    +  + F +   + ++++KAS+++A  IA  SK F++G F+ +C+  V + +CPD       + LS  +IA R++++  + +         F +Y L +   T L  +    L ++  +  T    D+ + V K
Sbjct:   40 LVVKEYNLRSHLDTKHGAKY-AKASLQEKKQIAQELKGKLQSQQSFFTKSTVKEEASVKASFIMAEHIAHASKSFSEGAFLGQCMLKVYEQVCPDQLQTFKNVSLSQNTIAGRVKEVRHAAKR--------FFFYLLLMKAQTTLDLSITEKLLDVAAIHGTTTGQDIVDAVEK 204

[Close]

BLAST of SMED30002222 vs. Planmine SMEST
Match: SMESG000037770.1 (SMESG000037770.1)

HSP 1 Score: 112.464 bits (280), Expect = 6.552e-28
Identity = 67/193 (34.72%), Postives = 108/193 (55.96%), Query Frame = -3

Query:   52 DYNLKRHYFQKH----VSTYDAYNGMYSKDKVAELKSVFSTNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY---------------NITQVLATNINTDLYENVNKIL--FEL 567
            +YNLKRH+  KH    ++  +      + D V  LK      IF +  +  ++A KAS V+A+ I K +KPF++GEF+K+C+  +ADIICP+ +     I LS +++ RRIE I + +  +L N    F ++S+A+DESTD+ DTAQ+ ++               ++  +  T    DL++N+ K L  FEL
Sbjct:  567 EYNLKRHFQTKHGNFGINLNETELRRKADDLVRSLKQ--EQTIFLKPSSIQEAATKASLVIAHKIVKHNKPFSEGEFVKDCVLEIADIICPENKKKFENISLSRRTVVRRIESIAEDLSDQLLNKIKTFQWFSIALDESTDIQDTAQLLIFIRGIDDKFIATEELLSMEHLKDTTTGQDLFDNLIKALDKFEL 757

[Close]

BLAST of SMED30002222 vs. Planmine SMEST
Match: SMESG000015269.1 (SMESG000015269.1)

HSP 1 Score: 111.309 bits (277), Expect = 1.341e-27
Identity = 65/197 (32.99%), Postives = 112/197 (56.85%), Query Frame = -3

Query:   49 VSDYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFST-----NIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY--------NITQVLA-------TNINTDLYENVNKIL--FELD 573
            + +YN++RH+  KH STY   + +  KD++ + +++  +     N+F   +++N++    S  +A ++AK  KPF DG+  KEC+      +CP+  +    I LS  +IARRI+DIG +I  ++ + +  F  YSLA+++STD++DT+Q+ ++        N+TQ LA       T    D++E V K L  F LD
Sbjct:  130 MKEYNIRRHHETKHSSTY---SQLKVKDRIEKFEAMHRSLCSQQNLFRNANSENEALTLVSMKIAYLLAKAGKPFMDGQIFKECMMEAVGELCPEKLNLFKMISLSRHTIARRIDDIGNNILHQVTSKTQRFTQYSLAMNKSTDVNDTSQLLIFVRGVDDELNVTQELASVNSMHSTTTGKDIFEEVEKTLLKFNLD 323

[Close]

BLAST of SMED30002222 vs. Planmine SMEST
Match: SMESG000045419.1 (SMESG000045419.1)

HSP 1 Score: 96.6709 bits (239), Expect = 1.509e-22
Identity = 56/149 (37.58%), Postives = 84/149 (56.38%), Query Frame = -3

Query:  127 VSDYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSVFSTNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 573
            + +YN+KRHY ++H   Y    G   K+K       F T             LKAS      I  K +PFTD EF+K CL  VA+ +CP+T++    I LS ++ ARR+E+IG ++  +L   +  F+ YSLA+DES D++DTAQ+ ++
Sbjct:   44 MKEYNVKRHYEKEHERQYKDLTGEIRKNK-------FRT-------------LKASLTAQQSIFMK-RPFTDLEFVKRCLVAVAEKLCPETKTLFQDISLSARTCARRVEEIGTNLFEQLKCKAKSFDCYSLAMDESIDITDTAQLIIF 171

[Close]

BLAST of SMED30002222 vs. Planmine SMEST
Match: SMESG000060595.1 (SMESG000060595.1)

HSP 1 Score: 70.4774 bits (171), Expect = 5.685e-15
Identity = 44/136 (32.35%), Postives = 72/136 (52.94%), Query Frame = -3

Query:  121 MYSKDKVAELKSVFST--NIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIIC------PDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALYNI 504
            M  K+K+ +LK+  +    +F R    +  A  AS+ +++++AKK KPF DGE  KE      D +        +  S I  ++LS+ ++  RI  I   I+T+L     I +++ L  DESTD+SDT Q+ +  I
Sbjct:    4 MCVKEKICKLKASLTVKQTVFTRHVEYSKKATIASFKISHMLAKKKKPFIDGELFKELFLTGVDCLFDRFSNKHEIVSAIQDLQLSNNTVTIRIHAISTDIQTQLKTDLEICDWFWLQFDESTDISDTEQLEVMMI 139

[Close]

BLAST of SMED30002222 vs. Planmine SMEST
Match: SMESG000018070.1 (SMESG000018070.1)

HSP 1 Score: 73.9442 bits (180), Expect = 9.114e-15
Identity = 51/152 (33.55%), Postives = 77/152 (50.66%), Query Frame = -3

Query:  127 AVSDYNLKRHYFQKHVSTYDAYNGMYSKDKVAELKSV--FSTNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDGEFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNGSVIFNYYSLAIDESTDLSDTAQVALY 576
             + +YN+KRH+  KH   Y   N  + K+ ++ELKS   +   IF+       + +KASY V  +IAKK KPF D E IKECL  V           +  + L    ++  I +I  ++  R     V    YSL  +ESTD+SD +Q+ ++
Sbjct:  850 PIKEYNVKRHHETKH-DVYVKSNKEWEKNSLSELKSELKYQQKIFSSSLQLASNIVKASYSVTLLIAKKMKPFLDRECIKECLAAV-----------LEDVMLDKHKMSSSINEIVMTLRERFQQSKV----YSLVFNESTDISDASQLVVF 985

The following BLAST results are available for this feature:

BLAST of SMED30002222 vs. Ensembl Human
BLAST of SMED30002222 vs. Ensembl Celegans
BLAST of SMED30002222 vs. Ensembl Fly
BLAST of SMED30002222 vs. Ensembl Zebrafish
BLAST of SMED30002222 vs. Ensembl Xenopus
BLAST of SMED30002222 vs. Ensembl Mouse
BLAST of SMED30002222 vs. UniProt/SwissProt
BLAST of SMED30002222 vs. TrEMBL
BLAST of SMED30002222 vs. Ensembl Cavefish
BLAST of SMED30002222 vs. Ensembl Sea Lamprey
BLAST of SMED30002222 vs. Ensembl Yeast
BLAST of SMED30002222 vs. Ensembl Nematostella
BLAST of SMED30002222 vs. Ensembl Medaka
BLAST of SMED30002222 vs. Planmine SMEST

BLAST of SMED30002222 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ZBED9	2.532e-13	30.92	zinc finger BED-type containing 9 [Source:HGNC Sym...	[more]
ZBED9	7.761e-13	30.92	zinc finger BED-type containing 9 [Source:HGNC Sym...	[more]
ZBED5	1.344e-6	34.58	zinc finger BED-type containing 5 [Source:HGNC Sym...	[more]
ZBED5	1.344e-6	34.58	zinc finger BED-type containing 5 [Source:HGNC Sym...	[more]
EPM2AIP1	1.366e-6	23.03	EPM2A interacting protein 1 [Source:HGNC Symbol;Ac...	[more]

BLAST of SMED30002222 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30002222 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30002222 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
AL928808.1	7.608e-20	32.24	pep chromosome:GRCz11:20:17257101:17259573:-1 gene...	[more]
CR392001.3	5.738e-6	28.34	pep chromosome:GRCz11:8:38963323:38965260:-1 gene:...	[more]

BLAST of SMED30002222 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4


Match Name	E-value	Identity	Description
spag1	2.527e-22	35.29	sperm associated antigen 1 [Source:Xenbase;Acc:XB-...	[more]
ENSXETT00000016563.1	2.771e-22	35.29	pep primary_assembly:Xenopus_tropicalis_v9.1:4:373...	[more]
ENSXETT00000028445.1	5.574e-13	30.97	general transcription factor II-I repeat domain-co...	[more]
ENSXETT00000010836.1	1.238e-6	46.43	pep primary_assembly:Xenopus_tropicalis_v9.1:7:638...	[more]

BLAST of SMED30002222 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3


Match Name	E-value	Identity	Description
Epm2aip1	1.001e-7	24.38	EPM2A (laforin) interacting protein 1 [Source:MGI ...	[more]
Epm2aip1	1.001e-7	24.38	EPM2A (laforin) interacting protein 1 [Source:MGI ...	[more]
Zbed5	4.986e-7	29.05	zinc finger, BED type containing 5 [Source:MGI Sym...	[more]

BLAST of SMED30002222 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q6R2W3\|SCND3_HUMAN	3.727e-12	30.92	SCAN domain-containing protein 3 OS=Homo sapiens O...	[more]
sp\|A4IFA3\|GT2D2_BOVIN	2.431e-7	23.81	General transcription factor II-I repeat domain-co...	[more]
sp\|Q8VEH5\|EPMIP_MOUSE	7.002e-7	24.38	EPM2A-interacting protein 1 OS=Mus musculus OX=100...	[more]
sp\|Q49AG3\|ZBED5_HUMAN	6.454e-6	34.58	Zinc finger BED domain-containing protein 5 OS=Hom...	[more]
sp\|A4Z943\|ZBED5_BOVIN	6.516e-6	34.58	Zinc finger BED domain-containing protein 5 OS=Bos...	[more]

BLAST of SMED30002222 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
J9JJ25	3.431e-37	43.98	DUF4371 domain-containing protein OS=Acyrthosiphon...	[more]
A0A1S4G7B0	5.155e-36	42.17	Uncharacterized protein OS=Aedes aegypti OX=7159 P...	[more]
A0A0P6J0U1	3.953e-35	40.96	Proteinral transcription factor ii-i repeat domain...	[more]
A0A2T7P2Y2	4.778e-34	36.17	Uncharacterized protein OS=Pomacea canaliculata OX...	[more]
A0A3B1J537	4.613e-33	43.62	DUF4371 domain-containing protein OS=Astyanax mexi...	[more]

BLAST of SMED30002222 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000030525.1	1.747e-36	43.62	pep primary_assembly:Astyanax_mexicanus-2.0:1:1299...	[more]
ENSAMXT00000035253.1	9.428e-27	36.18	general transcription factor II-I repeat domain-co...	[more]
ENSAMXT00000052542.1	2.341e-26	37.58	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
ENSAMXT00000038753.1	2.544e-26	33.77	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
ENSAMXT00000037725.1	1.449e-25	36.91	pep primary_assembly:Astyanax_mexicanus-2.0:14:291...	[more]

BLAST of SMED30002222 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30002222 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30002222 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30002222 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSORLT00000027093.1	1.384e-32	35.98	general transcription factor II-I repeat domain-co...	[more]
ENSORLT00000033014.1	8.106e-32	38.51	general transcription factor II-I repeat domain-co...	[more]
ENSORLT00000028655.1	3.820e-28	34.21	pep primary_assembly:ASM223467v1:8:18412528:184146...	[more]
ENSORLT00000044825.1	2.242e-15	28.49	general transcription factor II-I repeat domain-co...	[more]
ENSORLT00000038351.1	1.201e-14	27.01	pep primary_assembly:ASM223467v1:3:5078117:5081344...	[more]

BLAST of SMED30002222 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000037770.1	6.552e-28	34.72	SMESG000037770.1	[more]
SMESG000015269.1	1.341e-27	32.99	SMESG000015269.1	[more]
SMESG000045419.1	1.509e-22	37.58	SMESG000045419.1	[more]
SMESG000060595.1	5.685e-15	32.35	SMESG000060595.1	[more]
SMESG000018070.1	9.114e-15	33.55	SMESG000018070.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30002222 ID=SMED30002222|Name=SMED30002222|organism=Schmidtea mediterranea sexual|type=transcript|length=770bp
AAGACCTTCCTTTTTTCAACCATCGCTGGGACACCATCAGGCATATGGAT
CAAGTTCAAATAAAATTTTGTTAACGTTTTCGTACAAATCTGTGTTGATG
TTGGTAGCCAACACTTGTGTGATATTATATAAAGCAACCTGAGCAGTATC
ACTGAGATCAGTACTTTCATCTATTGCTAAAGAATAATAATTAAAAATAA
CAGATCCATTTCCCAACCGTGTTTCAATGGATTTTCCAATATCTTCAATT
CGTCTTGCTATAGATTGGTGTGATAACCTGATTTTTGATATGTACGATTG
TGTATCTGGACAAATAATGTCAGCTACATTTTCCAAACATTCCTTAATAA
ATTCGCCATCTGTGAAGGGTTTCGACTTCTTTGCAATTATATTTGCTACC
ACATAACTGGCTTTCAAGGCAGAATCATTTTGATTGTCAATTCTATTAAA
AATGTTTGTTGAGAACACAGACTTCAGTTCAGCTACTTTATCTTTGCTAT
ACATTCCGTTGTAAGCATCATACGTTGAAACATGTTTTTGAAAATAATGT
CGTTTCAAATTATAATCTGAAACTGCTAGTTAGAATTATATTCACGTCGT
GTACGTACAAAATTTAATTATCCCGCAGTCGCATTTTACATTTTAATCTT
AATTCCTGAAGAAAAAAATCTAGGTGAAGCCTGTTTGTGTATTAATATAC
TATAACAGTATTTTTATTTATTCCCTGCTATTGGAGTATTATATTTCCAG
GAAAGTAAATGTTATTTAAA

protein sequence of SMED30002222-orf-1

>SMED30002222-orf-1 ID=SMED30002222-orf-1|Name=SMED30002222-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=156bp
MYSKDKVAELKSVFSTNIFNRIDNQNDSALKASYVVANIIAKKSKPFTDG
EFIKECLENVADIICPDTQSYISKIRLSHQSIARRIEDIGKSIETRLGNG
SVIFNYYSLAIDESTDLSDTAQVALYNITQVLATNINTDLYENVNKILFE
LDPYA*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000026	gut
PLANA:0000070	intestinal phagocyte

Main menu

SMED30002222

Zeng et. al., 2018

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Owlery

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