DUF862 domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30002215 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of DUF862 domain-containing protein vs. TrEMBL
Match: E9GQZ7 (Uncharacterized protein OS=Daphnia pulex OX=6669 GN=DAPPUDRAFT_305262 PE=4 SV=1) HSP 1 Score: 55.8398 bits (133), Expect = 3.582e-6 Identity = 38/133 (28.57%), Postives = 64/133 (48.12%), Query Frame = 3 Query: 162 DLSKPILHVWKYKTPMHWIQLTSFYFYHIFIVIETDEFFWSVEKNNLGIWIQKGRFKNDVISKMAGEER--------NLVICLEDLQGDFGQFRNLVDWIEINELQKGYSCNDYDF---NSKGFGDKVYDNII 527 D S+ I V Y P++W Q++ + +H FIV+ET+ ++WS+EK+ GI IQ+ + +V+ + R NL + E ++V+ + E DY F N K F + +YDN + Sbjct: 48 DSSEKINEVRYYTHPLNWWQMSKYVLHHAFIVLETESWWWSIEKDEGGITIQRSKHLENVLDQCRRHGRITGLATWINLGVTCEKKGPSHASVIDVVNRMWREECLHS----DYHFLQNNCKHFANTIYDNFV 176
BLAST of DUF862 domain-containing protein vs. TrEMBL
Match: E9GQ32 (Uncharacterized protein OS=Daphnia pulex OX=6669 GN=DAPPUDRAFT_320721 PE=4 SV=1) HSP 1 Score: 55.0694 bits (131), Expect = 5.989e-6 Identity = 45/160 (28.12%), Postives = 71/160 (44.38%), Query Frame = 3 Query: 69 ESDDWEGRIFFIRDTNCPNELYNGGEMKRQFDLSKPILHVWKYKTPMHWIQLTSFYFYHIFIVIETDEFFWSVEKNNLGIWIQKGRFKNDVISKMAGEERNLVICLEDLQGD--------FGQFRNLVDWIEINELQKGYSCNDYDF---NSKGFGDKVY 515 E+D GR FFI + + + G+ S IL V Y P++ +Q+T +H FIV T+ +WS+E+N LG+ IQ+ + V K ++R DL G FG + + + ++ Y DYD N K F + +Y Sbjct: 25 EADSSTGRDFFITAADLRKGVESLGK-------SDNILEVRYYTNPLYGVQVTQLVLHHAFIVFLTERAWWSIERNELGVTIQRSKKIEFVRDKFRRQDRTT-----DLFGSGIHWEKRCFGITNVTLKELLTHICREDYLNEDYDLLENNCKHFAENIY 172
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000024669.1 (SMESG000024669.1) HSP 1 Score: 330.102 bits (845), Expect = 3.004e-117 Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 3 Query: 60 MCFESDDWEGRIFFIRDTNCPNELYNGGEMKRQFDLSKPILHVWKYKTPMHWIQLTSFYFYHIFIVIETDEFFWSVEKNNLGIWIQKGRFKNDVISKMAGEERNLVICLEDLQGDFGQFRNLVDWIEINELQKGYSCNDYDFNSKGFGDKVYDNIINKQF 539 MCFESDDWEGRIFFIRDTNCPNELYNGGEMKRQFDLSKPILHVWKYKTPMHWIQLTSFYFYHIFIVIETDEFFWSVEKNNLGIWIQKGRFKNDVISKMAGEERNLVICLEDLQGDFGQFRNLVDWIEINELQKGYSCNDYDFNSKGFGDKVYDNIINKQF Sbjct: 1 MCFESDDWEGRIFFIRDTNCPNELYNGGEMKRQFDLSKPILHVWKYKTPMHWIQLTSFYFYHIFIVIETDEFFWSVEKNNLGIWIQKGRFKNDVISKMAGEERNLVICLEDLQGDFGQFRNLVDWIEINELQKGYSCNDYDFNSKGFGDKVYDNIINKQF 160
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000024663.1 (SMESG000024663.1) HSP 1 Score: 155.606 bits (392), Expect = 2.640e-48 Identity = 68/150 (45.33%), Postives = 105/150 (70.00%), Query Frame = 3 Query: 78 DWEGRIFFIRDTNCPNELYNGGEMKRQFDLSKPILHVWKYKTPMHWIQLTSFYFYHIFIVIETDEFFWSVEKNNLGIWIQKGRFKNDVISKMAGEERNLVICLEDLQGDFGQFRNLVDWIEINELQKGYSCNDYDFNSKGFGDKVYDNII 527 +WE RI FI +T+ PNE++NG EM++ D S+ I+ VWKYK + W Q+TSFY YH F++ ET++++WS+EKN GIWIQK + K+++++K+ G ERN L+ QG F +N+++WIE EL+ Y + D N +GF D+ Y++I+ Sbjct: 13 NWESRIHFINNTSAPNEVFNGSEMRKLIDTSETIVKVWKYKEKLVWWQITSFYLYHAFVIFETNDWYWSMEKNGSGIWIQKSKSKDNLVAKIGGNERNSTTLLDSCQGRFVTIKNIIEWIEKEELKSAYWYMNSDDNCQGFADRGYNHIV 162
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000024661.1 (SMESG000024661.1) HSP 1 Score: 143.665 bits (361), Expect = 1.219e-43 Identity = 65/150 (43.33%), Postives = 98/150 (65.33%), Query Frame = 3 Query: 78 DWEGRIFFIRDTNCPNELYNGGEMKRQFDLSKPILHVWKYKTPMHWIQLTSFYFYHIFIVIETDEFFWSVEKNNLGIWIQKGRFKNDVISKMAGEERNLVICLEDLQGDFGQFRNLVDWIEINELQKGYSCNDYDFNSKGFGDKVYDNII 527 +W RIFFI +T+ PN+++ G EM++ D S+ I+ WKYK + W Q+TSF+ YH F++ ET +++WS+EKN GIWIQK + KN++++K G ERN L+ G+F L++W+E NEL Y + FN +GF D+ Y +II Sbjct: 13 NWLSRIFFINNTSAPNDVFKGSEMRKLIDTSETIIQAWKYKQKLVWFQITSFFLYHAFVIFETKDWYWSIEKNGSGIWIQKSKSKNELVTKNGGNERNSTTLLDSEFGNFITIGELIEWVENNELINRYFYIETYFNCQGFADRAYQHII 162
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000024649.1 (SMESG000024649.1) HSP 1 Score: 129.798 bits (325), Expect = 2.667e-38 Identity = 66/159 (41.51%), Postives = 97/159 (61.01%), Query Frame = 3 Query: 54 LAMCFESDDWEGRIFFIRDTNCPNELYNGGEMKRQFDLSKPILHVWKYKTPMHWIQLTSFYFYHIFIVIETDEFFWSVEKNNLGIWIQKGRFKNDVISKMAGEERNLVICLEDLQGDFGQFRNLVDWIEINELQKGYSCNDYDFNSKGFGDKVYDNIIN 530 +++ + WE ++F I D PN LY G +MK+ +LS+ I VW+YK P+ QLTS FYHIF+V++TD++ WS+EKN GIW+QK N+++ K G ER V L G+F + ++L DWI NEL+ Y D +N KGF ++ + I N Sbjct: 1 MSLDIKGGKWESQLFLINDIRHPNNLYYGSQMKQMGNLSERIQQVWRYKEPLKSWQLTSVIFYHIFLVLQTDKWNWSIEKNGTGIWVQKSENHNELLKKKGGVERKSVSLLGHRIGNFVRIKSLFDWIIKNELKIKYYWLDSYYNCKGFVERAFKFITN 159
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000070711.1 (SMESG000070711.1) HSP 1 Score: 92.8189 bits (229), Expect = 7.416e-24 Identity = 48/154 (31.17%), Postives = 86/154 (55.84%), Query Frame = 3 Query: 78 DWEGRIFFIRDTNCPNELYNGGEMKRQFDLSKPILHVWKYKTPMHWIQLTSFYFY-----HIFIVIETDEFFWSVEKNNLGIWIQKGRFKNDVISKMAGEERNLVICLEDLQGDFGQFRNLVDWIEINELQKGYSCNDYDFNSKGFGDKVYDNI 524 +WEG+IF+I D P E+YN E+ ++ + I+ + YKTP+ + Y H F+V++T F++S +KN GI+IQK K+DV+ + G++R+ + L+ F ++ WI +EL++GY +++ N +GF +Y + Sbjct: 6 NWEGKIFYIDDLERPEEIYNQHEILQKIGSKETIVEIDVYKTPLQGLDFVEEKVYEKIVHHAFVVVKTTNFYFSFDKNGRGIYIQKSENKDDVLKSVFGDQRSRPVKLKSNTNVRVDFDTIIKWIIRDELKRGYHILNFETNCQGFSASLYTKV 159 The following BLAST results are available for this feature:
BLAST of DUF862 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of DUF862 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 2
BLAST of DUF862 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30002215 ID=SMED30002215|Name=DUF862 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=590bpback to top protein sequence of SMED30002215-orf-1 >SMED30002215-orf-1 ID=SMED30002215-orf-1|Name=SMED30002215-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=161bp MCFESDDWEGRIFFIRDTNCPNELYNGGEMKRQFDLSKPILHVWKYKTPMback to top Annotated Terms
The following terms have been associated with this transcript:
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