SMED30002203

Overview
NameSMED30002203
Smed IDSMED30002203
Length (bp)4522
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30002203 (SMED30002203) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30002203 (SMED30002203) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30002203 (SMED30002203) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30002203 (SMED30002203) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30002203

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30002203h1SMcG0012501 dd_Smed_v4_8612_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
anterior sideSMED30002203h1SMcG0012501 dd_Smed_v4_8612_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
testisSMED30002203h1SMcG0012501 Contig4488uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30002203h1SMcG0012501 Contig4488newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30002203 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000547:58374..93155 -22149
Homology
BLAST of SMED30002203 vs. Planmine SMEST
Match: SMESG000073490.1 (SMESG000073490.1)

HSP 1 Score: 2164.81 bits (5608), Expect = 0.000e+0
Identity = 1119/1127 (99.29%), Postives = 1124/1127 (99.73%), Query Frame = 1
Query:   40 MIYSTWMSRLILKFKNYCGGISILLFLLNIFDFAEGSNFSILNSQGYKFRFLRKKSVEPKRNFCSENLENLLNYTFVKNKLSPALXXXXXXXXXXXXXXTTILLPQGNESVEQYLKLTPILAPIQRIKSNEKVILFPVTGCINLASYNLQSISTVNNKTERLSGVQIFIFNLDNHTDDKSTNIDLKILLYSTQEKALIQPIVIVLSSFLPRKFSLSAISFISHSQRPLHIILTDSKSEVEINDNQLLAKDLNKLFISTIDLDKDRYFIYSEKMDNFFFENFQKQLIKTSLFKYGFFFGSNANSLSSNRQIQKLWLFNDEKWFSIHKPNIRIAMIYIANRSLNAIELMIGKPIKTVNQFDSSRKNWRGNTCNSFKFLNQIVKTYIKVEYFVDSTRTCYYDVQSSLKTITNKYKTITWLHIESLPNVSLNIEFKSSHNMDLNKYSYLVILTENPKTFSKIALSSLFHDKVKILTNISLDRFDLNFSGNSSSIELQLISNSNDGSFQSQTIANYISAGSSGTMQTTVYSFAKTRPFYEISINIDDSKSVPEVIHQCTGNSHNGYLVLISINLSKWKLIGNWAEAEFYLGNSSCIFNKRRNKTIHITKEAIHCSSTYNDKLYYNKIYGIWRNKTYLLKTVYFLCKNNANQKSHATSTKESAIYIPKDENVINIFNNSDRKTFPEKIFYDKSRLFIQLVNLKPPFSFELRSCYLSNQMATFEDFKSSSEIDGSFKKMSIVNEGCLNKSSPWYLNIDKSQQPNELPNYNTEMSYYFFIEIDANIWAKKLYLENQWYTENKYEVHLYCKISPCFKKNSKLTSSCRSKEERCSKKLSNIDIEVKYYGSIVIAKKYENVFFFKIGILITXXXXXXXXXXXXXXXXXCHSLKQRHIQLEIVNKNPRLGQFNDEEVLFSSPRKRLMNLNYHSLTLLSNSNDKFSTNLYSEPKLYNSLKNLVANCDKSIYLNAGFLTFSPNVDNSQLLVSVVEPLYSPNKLKNKCRSLSEHSFTEPTMTNFVINSWPLSSVERKSISQGRKNSHQYSFQLSKTMKMKSNQHYNNLRHPTTNXXXXXXXXXXXXPFHSELKFNSNIKISSNRTTLSDSTSVNDIPNKSSSKWWSLLTNYWMNQSLVMSRN 3420
            MIYSTWMSRLILKFKNYCGGISILLFLLNIFDFAEGSNFSILNSQGYKFRFLRKKSVEPKRNFCSENLENLLNYTFVKNKLSPALSSNYKPSNKNYSSSTTILLPQGNESVEQYLKLTPILAPIQRIKSNEKVILFPVTGCINLASYNLQSISTVNNKTERLSGVQIFIFNLDNH DDKSTNIDLKILLYSTQEKALIQPIVIVLSSFLPRKFSLSAISFISHSQRPLHIILTDSKSEVEINDNQLLAKDLNKLFISTIDLDKDRYFIYSEKMDNFFFEN QKQLIKTSLFKYGFFFGSNANSLSSNRQIQKLWLFNDEKWFSIHKPNIRIAMIYIANRSLNAIELMIGKPIKTVNQFDSSRKNWRGNTCNSFKFLNQIVKTYIKVEYFVDSTRTCYYDVQSSLK+ITNKYKTITWLHIESLPNVSLNIEFKSSHNMDLNKYSYLVILTENPKTFSKIALSSLFHDKVKILTNISLDRFDLNFSGNSSSIELQLISNSNDGSFQSQTIANYISAGSSGTMQTTVYSFAKTRPFYEISI+IDDSKSVPEVIHQCTGNSHNGYLVLISINLSKWKLIGNWAEAEFYLGNSSCIFNKRRNKTIHITKEAIHCSSTYNDKLYYNKIYGIWRNKTYLLKTVYFLCKNNANQKSHAT+TKESAIYIPKDENVINIFNNSDRKTFPEKIFYDKSRLFIQLVNLKPPFSFELRSCYLSNQMATFEDFKSSSEIDGSFKKMSIVNEGCLNKSSPWYLNIDKSQQPNELPNYNTEMSYYFFIEIDANIWAKKLYLENQWYTENKYEVHLYCKISPCFKKNSKLTSSCRSKEERCSKKLSNIDIEVKYYGSIVIAKKYENVFFFKIGILITLLVIIFMLILSFILFCLCHSLKQRHIQLEIVNKNPRLGQFNDEEVLFSSPRKRLMNLNYHSLTLLSNSN+KFSTNLYSEPKLYNSLKNLVANCDKSIYLNAGFLTFSP+VDNSQLLVSVVEPLYSPNK KNKCRSLSEHSFTEPTMTNFVINSWPLSSVERKSISQGRKNSHQYSFQLSKTMKMKSNQHYNNLRHPTTNSSGGSSKYKISSPFHSELKFNSNIKISSNRTTLSDSTSVNDIPNKSSSKWWSLLTNYWMNQSLVMSRN
Sbjct:    1 MIYSTWMSRLILKFKNYCGGISILLFLLNIFDFAEGSNFSILNSQGYKFRFLRKKSVEPKRNFCSENLENLLNYTFVKNKLSPALSSNYKPSNKNYSSSTTILLPQGNESVEQYLKLTPILAPIQRIKSNEKVILFPVTGCINLASYNLQSISTVNNKTERLSGVQIFIFNLDNHPDDKSTNIDLKILLYSTQEKALIQPIVIVLSSFLPRKFSLSAISFISHSQRPLHIILTDSKSEVEINDNQLLAKDLNKLFISTIDLDKDRYFIYSEKMDNFFFENVQKQLIKTSLFKYGFFFGSNANSLSSNRQIQKLWLFNDEKWFSIHKPNIRIAMIYIANRSLNAIELMIGKPIKTVNQFDSSRKNWRGNTCNSFKFLNQIVKTYIKVEYFVDSTRTCYYDVQSSLKSITNKYKTITWLHIESLPNVSLNIEFKSSHNMDLNKYSYLVILTENPKTFSKIALSSLFHDKVKILTNISLDRFDLNFSGNSSSIELQLISNSNDGSFQSQTIANYISAGSSGTMQTTVYSFAKTRPFYEISISIDDSKSVPEVIHQCTGNSHNGYLVLISINLSKWKLIGNWAEAEFYLGNSSCIFNKRRNKTIHITKEAIHCSSTYNDKLYYNKIYGIWRNKTYLLKTVYFLCKNNANQKSHATATKESAIYIPKDENVINIFNNSDRKTFPEKIFYDKSRLFIQLVNLKPPFSFELRSCYLSNQMATFEDFKSSSEIDGSFKKMSIVNEGCLNKSSPWYLNIDKSQQPNELPNYNTEMSYYFFIEIDANIWAKKLYLENQWYTENKYEVHLYCKISPCFKKNSKLTSSCRSKEERCSKKLSNIDIEVKYYGSIVIAKKYENVFFFKIGILITLLVIIFMLILSFILFCLCHSLKQRHIQLEIVNKNPRLGQFNDEEVLFSSPRKRLMNLNYHSLTLLSNSNEKFSTNLYSEPKLYNSLKNLVANCDKSIYLNAGFLTFSPSVDNSQLLVSVVEPLYSPNKFKNKCRSLSEHSFTEPTMTNFVINSWPLSSVERKSISQGRKNSHQYSFQLSKTMKMKSNQHYNNLRHPTTNSSGGSSKYKISSPFHSELKFNSNIKISSNRTTLSDSTSVNDIPNKSSSKWWSLLTNYWMNQSLVMSRN 1127          
BLAST of SMED30002203 vs. Planmine SMEST
Match: SMESG000073490.1 (SMESG000073490.1)

HSP 1 Score: 2152.48 bits (5576), Expect = 0.000e+0
Identity = 1113/1121 (99.29%), Postives = 1118/1121 (99.73%), Query Frame = 1
Query:   58 MSRLILKFKNYCGGISILLFLLNIFDFAEGSNFSILNSQGYKFRFLRKKSVEPKRNFCSENLENLLNYTFVKNKLSPALXXXXXXXXXXXXXXTTILLPQGNESVEQYLKLTPILAPIQRIKSNEKVILFPVTGCINLASYNLQSISTVNNKTERLSGVQIFIFNLDNHTDDKSTNIDLKILLYSTQEKALIQPIVIVLSSFLPRKFSLSAISFISHSQRPLHIILTDSKSEVEINDNQLLAKDLNKLFISTIDLDKDRYFIYSEKMDNFFFENFQKQLIKTSLFKYGFFFGSNANSLSSNRQIQKLWLFNDEKWFSIHKPNIRIAMIYIANRSLNAIELMIGKPIKTVNQFDSSRKNWRGNTCNSFKFLNQIVKTYIKVEYFVDSTRTCYYDVQSSLKTITNKYKTITWLHIESLPNVSLNIEFKSSHNMDLNKYSYLVILTENPKTFSKIALSSLFHDKVKILTNISLDRFDLNFSGNSSSIELQLISNSNDGSFQSQTIANYISAGSSGTMQTTVYSFAKTRPFYEISINIDDSKSVPEVIHQCTGNSHNGYLVLISINLSKWKLIGNWAEAEFYLGNSSCIFNKRRNKTIHITKEAIHCSSTYNDKLYYNKIYGIWRNKTYLLKTVYFLCKNNANQKSHATSTKESAIYIPKDENVINIFNNSDRKTFPEKIFYDKSRLFIQLVNLKPPFSFELRSCYLSNQMATFEDFKSSSEIDGSFKKMSIVNEGCLNKSSPWYLNIDKSQQPNELPNYNTEMSYYFFIEIDANIWAKKLYLENQWYTENKYEVHLYCKISPCFKKNSKLTSSCRSKEERCSKKLSNIDIEVKYYGSIVIAKKYENVFFFKIGILITXXXXXXXXXXXXXXXXXCHSLKQRHIQLEIVNKNPRLGQFNDEEVLFSSPRKRLMNLNYHSLTLLSNSNDKFSTNLYSEPKLYNSLKNLVANCDKSIYLNAGFLTFSPNVDNSQLLVSVVEPLYSPNKLKNKCRSLSEHSFTEPTMTNFVINSWPLSSVERKSISQGRKNSHQYSFQLSKTMKMKSNQHYNNLRHPTTNXXXXXXXXXXXXPFHSELKFNSNIKISSNRTTLSDSTSVNDIPNKSSSKWWSLLTNYWMNQSLVMSRN 3420
            MSRLILKFKNYCGGISILLFLLNIFDFAEGSNFSILNSQGYKFRFLRKKSVEPKRNFCSENLENLLNYTFVKNKLSPALSSNYKPSNKNYSSSTTILLPQGNESVEQYLKLTPILAPIQRIKSNEKVILFPVTGCINLASYNLQSISTVNNKTERLSGVQIFIFNLDNH DDKSTNIDLKILLYSTQEKALIQPIVIVLSSFLPRKFSLSAISFISHSQRPLHIILTDSKSEVEINDNQLLAKDLNKLFISTIDLDKDRYFIYSEKMDNFFFEN QKQLIKTSLFKYGFFFGSNANSLSSNRQIQKLWLFNDEKWFSIHKPNIRIAMIYIANRSLNAIELMIGKPIKTVNQFDSSRKNWRGNTCNSFKFLNQIVKTYIKVEYFVDSTRTCYYDVQSSLK+ITNKYKTITWLHIESLPNVSLNIEFKSSHNMDLNKYSYLVILTENPKTFSKIALSSLFHDKVKILTNISLDRFDLNFSGNSSSIELQLISNSNDGSFQSQTIANYISAGSSGTMQTTVYSFAKTRPFYEISI+IDDSKSVPEVIHQCTGNSHNGYLVLISINLSKWKLIGNWAEAEFYLGNSSCIFNKRRNKTIHITKEAIHCSSTYNDKLYYNKIYGIWRNKTYLLKTVYFLCKNNANQKSHAT+TKESAIYIPKDENVINIFNNSDRKTFPEKIFYDKSRLFIQLVNLKPPFSFELRSCYLSNQMATFEDFKSSSEIDGSFKKMSIVNEGCLNKSSPWYLNIDKSQQPNELPNYNTEMSYYFFIEIDANIWAKKLYLENQWYTENKYEVHLYCKISPCFKKNSKLTSSCRSKEERCSKKLSNIDIEVKYYGSIVIAKKYENVFFFKIGILITLLVIIFMLILSFILFCLCHSLKQRHIQLEIVNKNPRLGQFNDEEVLFSSPRKRLMNLNYHSLTLLSNSN+KFSTNLYSEPKLYNSLKNLVANCDKSIYLNAGFLTFSP+VDNSQLLVSVVEPLYSPNK KNKCRSLSEHSFTEPTMTNFVINSWPLSSVERKSISQGRKNSHQYSFQLSKTMKMKSNQHYNNLRHPTTNSSGGSSKYKISSPFHSELKFNSNIKISSNRTTLSDSTSVNDIPNKSSSKWWSLLTNYWMNQSLVMSRN
Sbjct:    1 MSRLILKFKNYCGGISILLFLLNIFDFAEGSNFSILNSQGYKFRFLRKKSVEPKRNFCSENLENLLNYTFVKNKLSPALSSNYKPSNKNYSSSTTILLPQGNESVEQYLKLTPILAPIQRIKSNEKVILFPVTGCINLASYNLQSISTVNNKTERLSGVQIFIFNLDNHPDDKSTNIDLKILLYSTQEKALIQPIVIVLSSFLPRKFSLSAISFISHSQRPLHIILTDSKSEVEINDNQLLAKDLNKLFISTIDLDKDRYFIYSEKMDNFFFENVQKQLIKTSLFKYGFFFGSNANSLSSNRQIQKLWLFNDEKWFSIHKPNIRIAMIYIANRSLNAIELMIGKPIKTVNQFDSSRKNWRGNTCNSFKFLNQIVKTYIKVEYFVDSTRTCYYDVQSSLKSITNKYKTITWLHIESLPNVSLNIEFKSSHNMDLNKYSYLVILTENPKTFSKIALSSLFHDKVKILTNISLDRFDLNFSGNSSSIELQLISNSNDGSFQSQTIANYISAGSSGTMQTTVYSFAKTRPFYEISISIDDSKSVPEVIHQCTGNSHNGYLVLISINLSKWKLIGNWAEAEFYLGNSSCIFNKRRNKTIHITKEAIHCSSTYNDKLYYNKIYGIWRNKTYLLKTVYFLCKNNANQKSHATATKESAIYIPKDENVINIFNNSDRKTFPEKIFYDKSRLFIQLVNLKPPFSFELRSCYLSNQMATFEDFKSSSEIDGSFKKMSIVNEGCLNKSSPWYLNIDKSQQPNELPNYNTEMSYYFFIEIDANIWAKKLYLENQWYTENKYEVHLYCKISPCFKKNSKLTSSCRSKEERCSKKLSNIDIEVKYYGSIVIAKKYENVFFFKIGILITLLVIIFMLILSFILFCLCHSLKQRHIQLEIVNKNPRLGQFNDEEVLFSSPRKRLMNLNYHSLTLLSNSNEKFSTNLYSEPKLYNSLKNLVANCDKSIYLNAGFLTFSPSVDNSQLLVSVVEPLYSPNKFKNKCRSLSEHSFTEPTMTNFVINSWPLSSVERKSISQGRKNSHQYSFQLSKTMKMKSNQHYNNLRHPTTNSSGGSSKYKISSPFHSELKFNSNIKISSNRTTLSDSTSVNDIPNKSSSKWWSLLTNYWMNQSLVMSRN 1121          
The following BLAST results are available for this feature:
BLAST of SMED30002203 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002203 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002203 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002203 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002203 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002203 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002203 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002203 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002203 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002203 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002203 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002203 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002203 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002203 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000073490.10.000e+099.29SMESG000073490.1[more]
SMESG000073490.10.000e+099.29SMESG000073490.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002203 ID=SMED30002203|Name=SMED30002203|organism=Schmidtea mediterranea sexual|type=transcript|length=4522bp
ATCTATATAAATCTTATATGAACAACTCAGGAATATGAAATGATCTACTC
AACATGGATGTCTCGTTTAATATTAAAATTCAAGAATTATTGTGGTGGAA
TTAGCATTTTATTGTTTTTGTTAAATATTTTTGACTTCGCCGAAGGATCA
AATTTTAGCATCTTGAATTCTCAGGGTTATAAATTTCGTTTTCTCAGAAA
AAAAAGTGTTGAACCTAAAAGAAACTTTTGCAGTGAAAATTTAGAAAATT
TACTCAATTATACCTTTGTTAAAAACAAGCTGTCACCAGCATTGTCTTCC
AACTACAAGCCTTCAAATAAAAACTATTCTAGCTCAACCACTATTCTCCT
ACCTCAGGGAAATGAATCTGTCGAACAATATTTGAAATTAACACCAATTT
TAGCACCAATTCAAAGAATTAAGAGTAACGAGAAGGTTATACTTTTCCCG
GTGACTGGGTGCATAAATTTAGCAAGTTACAATCTGCAATCTATTAGTAC
AGTGAATAATAAAACAGAACGTCTTTCTGGTGTTCAGATTTTTATATTTA
ATCTAGATAACCACACAGATGATAAGTCAACAAATATTGATCTAAAAATA
TTATTATATTCTACTCAGGAAAAAGCACTCATTCAGCCAATTGTGATCGT
GCTCAGTAGCTTTCTACCTCGAAAGTTTTCTCTCTCAGCTATATCATTTA
TATCGCATTCTCAGCGGCCACTTCACATCATTCTAACTGACTCGAAAAGT
GAAGTGGAAATCAATGATAATCAATTACTTGCTAAAGATTTGAACAAGTT
ATTTATTAGTACTATTGATTTGGATAAAGACAGATATTTCATTTATTCTG
AGAAAATGGACAATTTTTTTTTTGAAAATTTTCAAAAACAGTTAATCAAA
ACCAGCTTGTTTAAATATGGCTTCTTTTTCGGTTCAAACGCTAATTCTCT
TTCATCAAACAGGCAAATTCAAAAATTATGGTTGTTTAATGATGAGAAAT
GGTTTTCAATACATAAACCAAATATTAGAATAGCAATGATTTATATTGCA
AATAGGTCTTTGAATGCTATAGAATTGATGATCGGTAAACCAATTAAAAC
AGTAAATCAATTTGATAGTTCTAGGAAAAATTGGAGAGGAAACACGTGTA
ATTCTTTCAAGTTTCTAAATCAAATTGTCAAAACCTATATAAAAGTTGAA
TATTTTGTCGATTCAACTCGAACTTGTTATTACGATGTTCAATCAAGTCT
AAAAACTATAACGAATAAATATAAAACAATTACATGGTTGCATATTGAAA
GCTTACCAAATGTTTCCTTAAACATCGAATTTAAATCTAGTCATAATATG
GATTTAAATAAGTATTCGTATTTAGTTATTCTTACAGAGAATCCAAAAAC
ATTCTCGAAGATCGCACTGAGTTCTCTGTTTCACGATAAAGTGAAAATTC
TAACTAATATATCATTAGATCGGTTTGATCTAAATTTCAGTGGTAATTCG
TCTTCAATTGAACTACAGTTAATCAGTAATTCAAATGATGGCTCATTTCA
AAGTCAAACAATAGCTAATTACATATCGGCAGGATCATCAGGTACCATGC
AAACCACAGTCTACAGTTTTGCTAAAACAAGGCCATTTTATGAAATCAGT
ATCAACATCGATGATTCTAAATCTGTTCCTGAAGTTATTCATCAATGCAC
AGGCAATAGTCATAATGGATATTTAGTTTTAATATCAATAAACTTATCAA
AATGGAAGTTGATTGGCAACTGGGCAGAGGCTGAATTTTATCTTGGGAAC
TCGAGTTGTATTTTTAACAAAAGAAGAAACAAAACTATTCATATCACAAA
AGAAGCAATTCATTGTTCTTCAACTTATAACGACAAGCTTTATTATAATA
AGATTTACGGAATTTGGAGAAATAAAACATATTTATTGAAAACCGTTTAT
TTTCTGTGCAAAAATAATGCTAATCAGAAATCACATGCAACTTCAACAAA
GGAAAGTGCGATTTACATACCAAAGGATGAAAATGTTATTAATATTTTCA
ATAATTCTGATAGAAAAACTTTTCCAGAAAAAATATTCTATGATAAATCA
CGATTATTTATACAATTGGTAAACCTTAAACCACCTTTTTCATTTGAGTT
GAGATCTTGTTATCTTTCAAATCAAATGGCCACGTTTGAGGATTTTAAAT
CATCATCAGAAATTGATGGGTCTTTTAAAAAAATGTCTATAGTTAACGAG
GGTTGTCTTAATAAATCTAGTCCTTGGTATTTAAATATCGATAAATCACA
ACAACCAAATGAATTGCCTAACTATAACACAGAAATGTCATATTATTTCT
TCATAGAAATTGATGCTAATATTTGGGCCAAAAAATTATATCTAGAAAAT
CAGTGGTACACAGAAAATAAATATGAAGTTCATTTATATTGTAAGATTAG
TCCATGCTTCAAGAAAAATTCTAAATTAACTTCATCGTGTCGGAGTAAAG
AAGAGCGTTGTTCTAAAAAATTATCAAATATAGATATTGAAGTAAAATAT
TATGGATCGATTGTTATCGCAAAGAAATATGAAAATGTATTTTTTTTCAA
AATTGGAATTCTTATTACACTCTTAGTGATAATCTTTATGCTTATATTGA
GTTTTATTTTATTCTGCTTATGTCATTCATTGAAGCAAAGACATATTCAA
TTAGAAATTGTTAATAAAAACCCTCGATTAGGACAATTTAATGATGAAGA
AGTACTATTTTCTAGTCCTCGTAAAAGACTTATGAATTTGAATTATCACA
GCCTGACATTGCTTTCAAATTCTAATGATAAATTTTCTACAAATTTGTAC
TCTGAACCAAAATTATATAATTCGTTAAAGAATTTAGTTGCGAATTGTGA
TAAGTCTATATATTTAAATGCTGGTTTCTTGACCTTTTCTCCTAATGTTG
ATAATTCTCAATTATTAGTGTCTGTAGTCGAACCTTTGTATTCACCAAAT
AAATTAAAAAATAAATGTCGTTCTCTATCTGAACATAGTTTTACAGAACC
AACTATGACTAATTTTGTTATTAATTCTTGGCCACTGTCATCTGTTGAAC
GCAAATCTATATCTCAGGGACGAAAAAATTCTCACCAATACAGTTTTCAA
CTTTCAAAAACCATGAAGATGAAATCTAATCAACATTATAATAACTTGAG
GCATCCAACTACTAATAGCAGCGGAGGTAGTAGTAAGTATAAAATTTCCA
GCCCCTTTCATTCGGAATTAAAATTTAACTCAAATATTAAAATATCTAGC
AATCGAACTACTCTCTCCGATTCTACATCGGTGAATGATATACCTAATAA
ATCTTCTTCAAAGTGGTGGTCACTGCTAACTAATTATTGGATGAATCAGA
GTTTAGTTATGTCTCGAAATTGATTTATGGATATCCAATCGACTGTGGCA
GCAGTGGTTCTATTTTCAACCCATTTTTATATATCCATTAAATAACTGCA
GGAATTTTTTTTCATTAAAATTATACCACTTGCACCCCTACTTGTGTAAC
TATTTTATGCATTTTACATCAGGAATTGTTTCTACCTCAGGGCACCATTT
AGAAAACATTTTTTCCTCTTATCAATTTGGTTTATAAAATCATTTTGTTT
CAACATTGCCGAATGTCAAACTGCATATTTGTAACCTCTCATGCTTTATT
GAACGTAAAAATGTATTACTAGTATTGTGTGATTTGGTATAGTTGGAGAG
TTATAACATTTTAATTTATCTCGATTGTATGTGTGTTTATAATATTCATT
ACTTTTTTCTGTATATTATTCATTGATCTTTAAGTTGTGTTTGGGAATTA
TTGTTTTTTGTTTTGATTGTGTCTGTAAATTAAGAGACCGATGCGAATGG
TGCCTCTTTGTTGACTCAGGATTTATTTTGCTTTTAGTTTTATTATTACT
GGTGTTATTGTTGATTTATACATTTTATCTCTGTAATGTAGTGTTACTGT
TAGTCATGTATTGATTTTAAGAAAACCAATAATCTGTGATGATATACACA
GCTGTGACTGTCCAGGCTTGCTCTTTTATGTAATCTATGTACATTTTTGA
CGAATCATCATGGCTTTTCATCATTTATCTGAGAAAAAATATAACATTTA
TGACATGTAAAATTTTCTTTTATCTGTAAACATAGTTTTAAATTTAATAT
ATTTTATTAAAGTTTTAATAAATCTTTAGAGATAAACCTTAAAAAATCAC
TTATTTTTATTTTTCCCTAGTTATTTGTGTGAAATTTTATTTTGAGCCTA
ATGAATGTCTTTGAATCAGGGATTGTACAGATTCTATTACTACAATTGCC
GTGTTGTACTAAATTGTCGATTTATTGAATATTGCCAATGCATAGTTTGC
AGCATTATTTAGATAATTATAAATTATCGGGAATTCGTGTGATACGTCAA
TTTTGCTTGTAAGCATGGAATATTTGTTGATATTTTACTTGCTGGATAAC
AATATAAGAATTTGATTTCGTT
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protein sequence of SMED30002203-orf-1

>SMED30002203-orf-1 ID=SMED30002203-orf-1|Name=SMED30002203-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1128bp
MIYSTWMSRLILKFKNYCGGISILLFLLNIFDFAEGSNFSILNSQGYKFR
FLRKKSVEPKRNFCSENLENLLNYTFVKNKLSPALSSNYKPSNKNYSSST
TILLPQGNESVEQYLKLTPILAPIQRIKSNEKVILFPVTGCINLASYNLQ
SISTVNNKTERLSGVQIFIFNLDNHTDDKSTNIDLKILLYSTQEKALIQP
IVIVLSSFLPRKFSLSAISFISHSQRPLHIILTDSKSEVEINDNQLLAKD
LNKLFISTIDLDKDRYFIYSEKMDNFFFENFQKQLIKTSLFKYGFFFGSN
ANSLSSNRQIQKLWLFNDEKWFSIHKPNIRIAMIYIANRSLNAIELMIGK
PIKTVNQFDSSRKNWRGNTCNSFKFLNQIVKTYIKVEYFVDSTRTCYYDV
QSSLKTITNKYKTITWLHIESLPNVSLNIEFKSSHNMDLNKYSYLVILTE
NPKTFSKIALSSLFHDKVKILTNISLDRFDLNFSGNSSSIELQLISNSND
GSFQSQTIANYISAGSSGTMQTTVYSFAKTRPFYEISINIDDSKSVPEVI
HQCTGNSHNGYLVLISINLSKWKLIGNWAEAEFYLGNSSCIFNKRRNKTI
HITKEAIHCSSTYNDKLYYNKIYGIWRNKTYLLKTVYFLCKNNANQKSHA
TSTKESAIYIPKDENVINIFNNSDRKTFPEKIFYDKSRLFIQLVNLKPPF
SFELRSCYLSNQMATFEDFKSSSEIDGSFKKMSIVNEGCLNKSSPWYLNI
DKSQQPNELPNYNTEMSYYFFIEIDANIWAKKLYLENQWYTENKYEVHLY
CKISPCFKKNSKLTSSCRSKEERCSKKLSNIDIEVKYYGSIVIAKKYENV
FFFKIGILITLLVIIFMLILSFILFCLCHSLKQRHIQLEIVNKNPRLGQF
NDEEVLFSSPRKRLMNLNYHSLTLLSNSNDKFSTNLYSEPKLYNSLKNLV
ANCDKSIYLNAGFLTFSPNVDNSQLLVSVVEPLYSPNKLKNKCRSLSEHS
FTEPTMTNFVINSWPLSSVERKSISQGRKNSHQYSFQLSKTMKMKSNQHY
NNLRHPTTNSSGGSSKYKISSPFHSELKFNSNIKISSNRTTLSDSTSVND
IPNKSSSKWWSLLTNYWMNQSLVMSRN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000140anterior region of the whole animal
PLANA:0003001lateral region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR006588Peptide_N_glycanase_PAW_dom
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
Vocabulary: biological process
TermDefinition
GO:0006516glycoprotein catabolic process