Ast1 protein

Overview


Name	Ast1 protein (CCW36839.1)
Smed ID	SMED30002195
Length (bp)	787

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Ast1 protein (SMED30002195) t-SNE clustered cells

Violin plots show distribution of expression levels for Ast1 protein (SMED30002195) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ast1 protein (SMED30002195) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ast1 protein (SMED30002195) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30002195

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
prepharyngeal region	SMED30002195	h1SMcG0000570	SmedASXL_009672	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
parapharyngeal region	SMED30002195	h1SMcG0000570	SmedASXL_009672	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
parapharyngeal region	SMED30002195	h1SMcG0000570	dd_Smed_v6_51_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
parapharyngeal region	SMED30002195	h1SMcG0000570	dd_Smed_v6_85_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
head region	SMED30002195	h1SMcG0000570	OX_Smed_1.0.03748	ox_Smed_v2	PMID:24238224 Kao et al., 2013

Note: Hover over icons to view figure legend

Homology

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BLAST of Ast1 protein vs. Ensembl Human
Match: ASTL (astacin like metalloendopeptidase [Source:HGNC Symbol;Acc:HGNC:31704])

HSP 1 Score: 90.5077 bits (223), Expect = 3.645e-20
Identity = 63/216 (29.17%), Postives = 98/216 (45.37%), Query Frame = 2

Query:  125 LLDKSDIALINGFKNHYANTKKWPNN-----NVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKF-TESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMY-SKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
             L + DI   + F+   A + KWP        V +  +  +      V+ + L E +R    C++F T  +Q  +I +     GC+S +G + GM  Q ++L  P C+   +G V HE +H LGF H H R DRD +I +NW  I       F K     +  PY+ +SVMHY    F+      +  L      IG   + + +D+  +   YGC
Sbjct:   72 FLIEGDIIRPSPFRLLSATSNKWPMGGSGVVEVPFLLSSKYDEPSRQVILEALAEFERS--TCIRFVTYQDQRDFISII-PMYGCFSSVGRSGGM--QVVSLA-PTCLQKGRGIVLHELMHVLGFWHEHTRADRDRYIRVNWNEILPGFEINFIKSQSSNMLTPYDYSSVMHYGRLAFSRRGLPTITPLWAPSVHIGQRWNLSASDITRVLKLYGC 281

[Close]

BLAST of Ast1 protein vs. Ensembl Human
Match: TLL1 (tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843])

HSP 1 Score: 75.485 bits (184), Expect = 5.007e-15
Identity = 59/197 (29.95%), Postives = 87/197 (44.16%), Query Frame = 2

Query:  185 KKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDG---PYETTSVMHYNSEGFACDPREKVVL--RKDGG---AIGYNHDTTPNDLNMIRNFYGC 748
            + WP   + Y   GNF   +  + +Q +   ++    CV F E S++  YI    +  GC S +G     P Q +++      +  G V HE  H +GF H H R DRDNH+TI    I+  Q   F K +  E++     Y+  S+MHY    F+       +L  R D G   AIG     +  D+   R  Y C
Sbjct:  155 RIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKH--TCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGP-QAISIGKNCDKF--GIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRC 346

[Close]

BLAST of Ast1 protein vs. Ensembl Human
Match: MEP1B (meprin A subunit beta [Source:HGNC Symbol;Acc:HGNC:7020])

HSP 1 Score: 75.485 bits (184), Expect = 7.318e-15
Identity = 66/218 (30.28%), Postives = 100/218 (45.87%), Query Frame = 2

Query:  116 GDMLLDKSDIALINGFKNHYANTK-KWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQF-TKCDGC--ELDGPYETTSVMHYNSEGFACDPREKVVLRKDG--GAIGYNHDTTPNDLNMIRNFYGC 748
            GD+ LD++ I      +N     K +WP+  + Y    +   +   V+       +  L  C+ F   + + +YI V  K  GC+S +G    +  Q L++    C     TV+HEF+HALGF H   R DRD+++ I W RI + +   F T  D     L+ PY+ TSVMHY+   F       +V R       IG   D + +DL  +   Y C
Sbjct:   51 GDIRLDRAQI------RNSIIGEKYRWPHT-IPYVLEDSLEMNAKGVILNAFERYR--LKTCIDFKPWAGETNYISV-FKGSGCWSSVGNRR-VGKQELSI-GANCD-RIATVQHEFLHALGFWHEQSRSDRDDYVRIMWDRILSGREHNFNTYSDDISDSLNVPYDYTSVMHYSKTAFQNGTEPTIVTRISDFEDVIGQRMDFSDSDLLKLNQLYNC 255

[Close]

BLAST of Ast1 protein vs. Ensembl Human
Match: MEP1B (meprin A subunit beta [Source:HGNC Symbol;Acc:HGNC:7020])

HSP 1 Score: 75.485 bits (184), Expect = 7.594e-15
Identity = 66/218 (30.28%), Postives = 100/218 (45.87%), Query Frame = 2

Query:  116 GDMLLDKSDIALINGFKNHYANTK-KWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQF-TKCDGC--ELDGPYETTSVMHYNSEGFACDPREKVVLRKDG--GAIGYNHDTTPNDLNMIRNFYGC 748
            GD+ LD++ I      +N     K +WP+  + Y    +   +   V+       +  L  C+ F   + + +YI V  K  GC+S +G    +  Q L++    C     TV+HEF+HALGF H   R DRD+++ I W RI + +   F T  D     L+ PY+ TSVMHY+   F       +V R       IG   D + +DL  +   Y C
Sbjct:   51 GDIRLDRAQI------RNSIIGEKYRWPHT-IPYVLEDSLEMNAKGVILNAFERYR--LKTCIDFKPWAGETNYISV-FKGSGCWSSVGNRR-VGKQELSI-GANCD-RIATVQHEFLHALGFWHEQSRSDRDDYVRIMWDRILSGREHNFNTYSDDISDSLNVPYDYTSVMHYSKTAFQNGTEPTIVTRISDFEDVIGQRMDFSDSDLLKLNQLYNC 255

[Close]

BLAST of Ast1 protein vs. Ensembl Human
Match: TLL1 (tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843])

HSP 1 Score: 74.7146 bits (182), Expect = 1.416e-14
Identity = 59/195 (30.26%), Postives = 86/195 (44.10%), Query Frame = 2

Query:  191 WPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDG---PYETTSVMHYNSEGFACDPREKVVL--RKDGG---AIGYNHDTTPNDLNMIRNFYGC 748
            WP   + Y   GNF   +  + +Q +   ++    CV F E S++  YI    +  GC S +G     P Q +++      +  G V HE  H +GF H H R DRDNH+TI    I+  Q   F K +  E++     Y+  S+MHY    F+       +L  R D G   AIG     +  D+   R  Y C
Sbjct:  157 WPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKH--TCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGP-QAISIGKNCDKF--GIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRC 346

[Close]

BLAST of Ast1 protein vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 98.2117 bits (243), Expect = 7.134e-24
Identity = 61/195 (31.28%), Postives = 97/195 (49.74%), Query Frame = 2

Query:  185 KKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE---SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCD---GCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 748
            ++WPNN + Y+ +  +      V+   + E       CVKF     S    Y+ ++   EGCYSL+G   G   QP++L++ GC+   GT+ HE +HA+GF H   R+DRD++I + W  +      QF K +      LD PY+  S+MHY    F+   ++ +V +K G   +G     +  D+  I   Y C
Sbjct:  102 RRWPNNEIPYTLSSQYGSYARSVIANAMNEYH--TKTCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGG--KQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289

[Close]

BLAST of Ast1 protein vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 97.4413 bits (241), Expect = 2.579e-23
Identity = 61/195 (31.28%), Postives = 97/195 (49.74%), Query Frame = 2

Query:  185 KKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE---SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCD---GCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 748
            ++WPNN + Y+ +  +      V+   + E       CVKF     S    Y+ ++   EGCYSL+G   G   QP++L++ GC+   GT+ HE +HA+GF H   R+DRD++I + W  +      QF K +      LD PY+  S+MHY    F+   ++ +V +K G   +G     +  D+  I   Y C
Sbjct:  102 RRWPNNEIPYTLSSQYGSYARSVIANAMNEYH--TKTCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGG--KQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289

[Close]

BLAST of Ast1 protein vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 97.4413 bits (241), Expect = 2.770e-23
Identity = 64/205 (31.22%), Postives = 100/205 (48.78%), Query Frame = 2

Query:  155 NGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE---SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCD---GCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 748
            N  K  Y   ++WPNN + Y+ +  +      V+   + E       CVKF     S    Y+ ++   EGCYSL+G   G   QP++L++ GC+   GT+ HE +HA+GF H   R+DRD++I + W  +      QF K +      LD PY+  S+MHY    F+   ++ +V +K G   +G     +  D+  I   Y C
Sbjct:   95 NAIKQIY---RRWPNNEIPYTLSSQYGSYARSVIANAMNEYH--TKTCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGG--KQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289

[Close]

BLAST of Ast1 protein vs. Ensembl Celegans
Match: nas-7 (Zinc metalloproteinase nas-7 [Source:UniProtKB/Swiss-Prot;Acc:P55113])

HSP 1 Score: 93.9745 bits (232), Expect = 5.099e-22
Identity = 66/232 (28.45%), Postives = 112/232 (48.28%), Query Frame = 2

Query:   74 RQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYAN--TKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGH--YIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELD---GPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            R++L  + +    + D    KSDI L    K +  +   K WPN  + Y+ + ++   +  ++ + + +       C++F     G   Y+ +  K +GC+S +G  +G+  Q L+L+N GCM    T+ HE +H +GF H H R DRDN I I W  I+     QF K D  +      PY+  S++HY+S  F+ +    ++ +     IG   D +  D++ I   Y C
Sbjct:   51 REELFGKHIPVEVVNDF---KSDIRLPRRHKRNGVSRAAKLWPNARIPYAISPHYSPHERALLAKAVKQYHE--KTCIRFVPRQTGEPDYLFIG-KVDGCFSEVGRTSGV--QVLSLDN-GCM-EYATIIHEMMHVVGFYHEHERWDRDNFIDIIWQNIDRGALDQFGKVDLSKTSYYGQPYDYKSILHYDSLAFSKNGFPTMLPKVKSATIGNARDFSDVDISKINRMYNC 272

[Close]

BLAST of Ast1 protein vs. Ensembl Celegans
Match: nas-6 (Zinc metalloproteinase nas-6 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3S9])

HSP 1 Score: 90.5077 bits (223), Expect = 7.799e-21
Identity = 65/207 (31.40%), Postives = 97/207 (46.86%), Query Frame = 2

Query:  146 ALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGC--ELDGP-YETTSVMHYNSEGFACDPREKVVLRKDG--GAIGYNHDTTPNDLNMIRNFYGC 748
             L N  KN       W    + Y     F  ++I ++ +     +R    C++F +   Q  Y+ +  K  GCYS +G   G   Q ++L   GC + +  V HE +H++GF H H R DRD+HI INW  I     SQF K      +L G  Y+  S+MHY+S  F+ + R  +   ++G    IG   D +P D+  I   Y C
Sbjct:   69 VLFNALKNKQLT---WEGGVIPYEMDTAFSPNEIKILEKAFDSYRR--TTCIRFEKREGQTDYLNI-VKGYGCYSQVGRTGG--KQEISLGR-GCFFHEIIV-HELMHSVGFWHEHSRADRDDHIKINWDNILPGMKSQFDKISAVLQDLQGENYDYKSIMHYDSTAFSRNGRNTIETVENGFTQVIGTAMDLSPLDIVKINKLYSC 265

[Close]

BLAST of Ast1 protein vs. Ensembl Fly
Match: CG6763 (gene:FBgn0039069 transcript:FBtr0084378)

HSP 1 Score: 124.79 bits (312), Expect = 2.138e-33
Identity = 67/215 (31.16%), Postives = 112/215 (52.09%), Query Frame = 2

Query:  116 GDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDG-PYETTSVMHYNSEGFACDPREKVVLRKDGGA--IGYNHDTTPNDLNMIRNFYGC 748
            GDML+ ++D+ + NG       + +WPN  V Y   GNF    +  +   +GE  R    C++F + S++  YI +     GC+S +G   G   Q +NL++PGC+   GT  HE +HALGF+H   R +RD ++ I +  +++S  + F K    E  G PY+  SVMHY+   F+ + +  ++  +  GA  +G  +  +  D+  +   Y C
Sbjct:   95 GDMLVPQTDLIMKNGLPTQ---SSRWPNGVVPYEIRGNFNARDMATIENAIGEYHRR--TCIRFVKRSSERDYISIRGDNSGCWSSVGRVGG--KQEVNLQSPGCLSRPGTAMHELMHALGFLHEQNRMERDGYVAIQYNNVQSSAMNNFEKAARTEAFGVPYDYGSVMHYSKNAFSINGQPTILAMQANGADKMGQRNGFSDYDIQKLNRMYDC 302

[Close]

BLAST of Ast1 protein vs. Ensembl Fly
Match: CG6763 (gene:FBgn0039069 transcript:FBtr0334497)

HSP 1 Score: 124.79 bits (312), Expect = 2.138e-33
Identity = 67/215 (31.16%), Postives = 112/215 (52.09%), Query Frame = 2

Query:  116 GDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDG-PYETTSVMHYNSEGFACDPREKVVLRKDGGA--IGYNHDTTPNDLNMIRNFYGC 748
            GDML+ ++D+ + NG       + +WPN  V Y   GNF    +  +   +GE  R    C++F + S++  YI +     GC+S +G   G   Q +NL++PGC+   GT  HE +HALGF+H   R +RD ++ I +  +++S  + F K    E  G PY+  SVMHY+   F+ + +  ++  +  GA  +G  +  +  D+  +   Y C
Sbjct:   95 GDMLVPQTDLIMKNGLPTQ---SSRWPNGVVPYEIRGNFNARDMATIENAIGEYHRR--TCIRFVKRSSERDYISIRGDNSGCWSSVGRVGG--KQEVNLQSPGCLSRPGTAMHELMHALGFLHEQNRMERDGYVAIQYNNVQSSAMNNFEKAARTEAFGVPYDYGSVMHYSKNAFSINGQPTILAMQANGADKMGQRNGFSDYDIQKLNRMYDC 302

[Close]

BLAST of Ast1 protein vs. Ensembl Fly
Match: CG11865 (gene:FBgn0028947 transcript:FBtr0080753)

HSP 1 Score: 92.8189 bits (229), Expect = 2.330e-22
Identity = 60/189 (31.75%), Postives = 89/189 (47.09%), Query Frame = 2

Query:  191 WPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKF--TESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFT--KCDGCELDG-PYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFY 742
            W    +VYS+AG F    I  +   + E+      CVKF  TE  +   + +  +  GC+S +GY  G   Q LNL   GCM S  T++HE +HALGF HTH    RD ++ I    I +     F   + +G    G  Y+  S+MHY    F+ + +  +V  K    IG     +P D+  ++  Y
Sbjct:   56 WKGRTLVYSYAGGFSSLDIASIESAMAEISSK--TCVKFRRTEYKREPQVVIQKEGSGCWSYVGYL-GRADQTLNL-GSGCM-SNRTIQHELLHALGFFHTHSDPQRDKYVRIQTDNIRSGHEHNFQRLRANGVTNYGFGYDYDSIMHYGPFAFSKNGQSTIVPLKSHAKIGQATQMSPKDVQTLKRMY 239

[Close]

BLAST of Ast1 protein vs. Ensembl Fly
Match: CG15254 (gene:FBgn0028949 transcript:FBtr0343718)

HSP 1 Score: 85.8853 bits (211), Expect = 1.044e-19
Identity = 62/212 (29.25%), Postives = 106/212 (50.00%), Query Frame = 2

Query:  155 NGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE--SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLE----NPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDG---PYETTSVMHYNSEGFACDPREKVVLRKDGGA--IGYNHDTTPNDLNMIRNFYGC*RR 757
            NG +N    T +WPN  V Y    +   +    + + +  +++   +C+ F E  ++Q +Y+ V S+  GCYS +GY   +  Q LNL+    + GC +  GT+ HEF+HALGF H     +RD+++ I    I       F K D   ++    PY+ +SV+HY +  F+ +    +V  ++G    +G     T +D+N +   Y C R+
Sbjct:   50 NGLRNE---TFRWPNRIVYYYINRDIDTEHRNHILRGIRIIEQ--SSCLVFKEATTDQEYYVNVTSEAGGCYSYVGYRNRV--QQLNLQTYALDTGC-FRLGTIVHEFLHALGFYHQQSTWNRDDYVRIAEENITEGTEGNFNKYDNETVEDYGEPYDYSSVLHYTAYAFSKNGEMTIVPLQEGAEELMGQRLQMTQSDINKLNVMYKCPRQ 253

[Close]

BLAST of Ast1 protein vs. Ensembl Fly
Match: CG15254 (gene:FBgn0028949 transcript:FBtr0080783)

HSP 1 Score: 85.8853 bits (211), Expect = 1.044e-19
Identity = 62/212 (29.25%), Postives = 106/212 (50.00%), Query Frame = 2

Query:  155 NGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE--SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLE----NPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDG---PYETTSVMHYNSEGFACDPREKVVLRKDGGA--IGYNHDTTPNDLNMIRNFYGC*RR 757
            NG +N    T +WPN  V Y    +   +    + + +  +++   +C+ F E  ++Q +Y+ V S+  GCYS +GY   +  Q LNL+    + GC +  GT+ HEF+HALGF H     +RD+++ I    I       F K D   ++    PY+ +SV+HY +  F+ +    +V  ++G    +G     T +D+N +   Y C R+
Sbjct:   50 NGLRNE---TFRWPNRIVYYYINRDIDTEHRNHILRGIRIIEQ--SSCLVFKEATTDQEYYVNVTSEAGGCYSYVGYRNRV--QQLNLQTYALDTGC-FRLGTIVHEFLHALGFYHQQSTWNRDDYVRIAEENITEGTEGNFNKYDNETVEDYGEPYDYSSVLHYTAYAFSKNGEMTIVPLQEGAEELMGQRLQMTQSDINKLNVMYKCPRQ 253

[Close]

BLAST of Ast1 protein vs. Ensembl Zebrafish
Match: hce2l2 (high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-146])

HSP 1 Score: 97.4413 bits (241), Expect = 1.113e-23
Identity = 73/272 (26.84%), Postives = 129/272 (47.43%), Query Frame = 2

Query:   11 MFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMG-DMLLDKSD---IALINGF--------------KNHYANTKKWP-----NNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNM--IRNFYGC 748
            +FL    +++   VS   G  RQ+     ++  +   D ++D +D   +  ++G               KN +A +  W      N  + YS +  +    +  +++ +  +++D   CV+F   ++Q  Y+++  K  GC+S LG   G   Q ++L++P C  S  TV HE +HALGF+H   R DRD ++TI W+ I   +   F K     LD PY+ +SVMH+    F+ D    +V +++   +       P+DL++  I   Y C
Sbjct:    9 LFLAGTCLSLPSQVSPNFGARRQRSYSEDIEENQTAMDRIIDVNDYQGVFSVDGTNLREGDIAVSGRSQKNCFARSCLWTKSVDGNVYIAYSLSHAYDDADVKNIKEGMELIEQD--TCVRFVPRTHQRDYLDIQPKT-GCWSYLGARGG--RQTISLQSPDCTGSGVTV-HELMHALGFVHEQSRADRDKYVTIMWSNIWKDRLRNFEKFKTNNLDTPYDYSSVMHFGKYAFSEDGEPTIVPKRNWN-VKIGQRLGPSDLDIMKINKLYSC 273

[Close]

BLAST of Ast1 protein vs. Ensembl Zebrafish
Match: he1.2 (hatching enzyme 1, tandem dupliate 2 [Source:ZFIN;Acc:ZDB-GENE-030131-2100])

HSP 1 Score: 97.0561 bits (240), Expect = 1.171e-23
Identity = 72/225 (32.00%), Postives = 105/225 (46.67%), Query Frame = 2

Query:   89 KRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNN--NVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV--LRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            K S + +  GD++L K+  ALI   K+ +   KK  NN   V Y  +G F  +   V+   +         C++F   S Q  Y+ + +K +GCYS +G   G   Q ++L   GC+YS G  +HE  HALGF H   R DRD ++ INW  I       F K      + PY+  S+MHY    FA  P  + +  +  +   IG     +  D+  I   YGC
Sbjct:   47 KGSSEVLFEGDVVLPKNRNALICEDKSCF--WKKNANNIVEVPYVVSGEFSINDKSVIANAISIFHAQ--TCIRFVPRSIQADYLSIENK-DGCYSAIGRTGG--KQVVSLNRKGCVYS-GIAQHELNHALGFYHEQSRSDRDQYVRINWNNISPGMAYNFLKQKTNNQNTPYDYGSLMHYGKTAFAIQPGLETITPIPDENVQIGQRQGLSKIDILRINKLYGC 263

[Close]

BLAST of Ast1 protein vs. Ensembl Zebrafish
Match: hce2l2 (high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-146])

HSP 1 Score: 95.1301 bits (235), Expect = 2.767e-23
Identity = 63/209 (30.14%), Postives = 109/209 (52.15%), Query Frame = 2

Query:  128 LDKSDIALI-NGFKNHYANTKKWP-----NNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIR 733
            L + DIA+     KN +A +  W      N  + YS +  +    +  +++ +  +++D   CV+F   ++Q  Y+++  K  GC+S LG   G   Q ++L++P C  S  TV HE +HALGF+H   R DRD ++TI W+ I   +   F K     LD PY+ +SVMH+    F+ D    +V +++   +       P+DL++++
Sbjct:    6 LREGDIAVSGRSQKNCFARSCLWTKSVDGNVYIAYSLSHAYDDADVKNIKEGMELIEQD--TCVRFVPRTHQRDYLDIQPKT-GCWSYLGARGGR--QTISLQSPDCTGSGVTV-HELMHALGFVHEQSRADRDKYVTIMWSNIWKDRLRNFEKFKTNNLDTPYDYSSVMHFGKYAFSEDGEPTIVPKRNWN-VKIGQRLGPSDLDIMK 207

[Close]

BLAST of Ast1 protein vs. Ensembl Zebrafish
Match: he1.1 (hatching enzyme 1, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-021211-3])

HSP 1 Score: 95.9005 bits (237), Expect = 3.468e-23
Identity = 71/225 (31.56%), Postives = 104/225 (46.22%), Query Frame = 2

Query:   89 KRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNN--NVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV--LRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            K S + +  GD++L K+  A I   K+ +   KK  NN   V Y  +G F  +   V+   +         C++F   S Q  Y+ + +K +GCYS +G   G   Q ++L   GC+YS G  +HE  HALGF H   R DRD ++ INW  I       F K      + PY+  S+MHY    FA  P  + +  +  +   IG     +  D+  I   YGC
Sbjct:   47 KGSSEVLFEGDVVLPKNRNAFICEDKSCF--WKKNANNIVEVPYVVSGEFSINDKSVIANAISIFHAQ--TCIRFVPRSIQADYLSIENK-DGCYSAIGRTGG--KQVVSLNRKGCVYS-GIAQHELNHALGFYHEQSRSDRDQYVRINWNNISPGMAYNFLKQKTNNQNTPYDYGSLMHYGKTAFAIQPGLETITPIPDENVQIGQRQGLSKIDILRINKLYGC 263

[Close]

Query:   89 KRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNN--NVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV--LRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            K S + +  GD++L K+  A I   K+ +   KK  NN   V Y  +G F  +   V+   +         C++F   S Q  Y+ + +K +GCYS +G   G   Q ++L   GC+YS G  +HE  HALGF H   R DRD ++ INW  I       F K      + PY+  S+MHY    FA  P  + +  +  +   IG     +  D+  I   YGC
Sbjct:   47 KGSSEVLFEGDVVLPKNRNAFICEDKSCF--WKKNANNIVEVPYVVSGEFSINDKSVIANAISIFHAQ--TCIRFVPRSIQADYLSIENK-DGCYSAIGRTGG--KQVVSLNRKGCVYS-GIAQHELNHALGFYHEQSRSDRDQYVRINWNNISPGMAYNFLKQKTNNQNTPYDYGSLMHYGKTAFAIQPGLETITPIPDENVQIGQRQGLSKIDILRINKLYGC 263

[Close]

BLAST of Ast1 protein vs. Ensembl Xenopus
Match: polr2l.2 (polymerase (RNA) II (DNA directed) polypeptide L, 7.6kDa, gene 2 [Source:Xenbase;Acc:XB-GENE-5898446])

HSP 1 Score: 97.0561 bits (240), Expect = 2.329e-22
Identity = 67/215 (31.16%), Postives = 106/215 (49.30%), Query Frame = 2

Query:  128 LDKSDIALINGFKNHYANTKKWPNN-----NVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFA-CDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 748
            L + DIA+  G       +  WP +      V Y  + ++  ++   +R  L  L+ +   CV F E S +  ++++ S   GC+S +G   G   Q L+L + GC+ +KG ++HE  HALGF H   R DRD +I + W  I  S    F   D   LD PY+ +SVMHY    F+    +  +  + D  A IG  +  +P D++ ++  YGC
Sbjct:   50 LWQGDIAIETGRSATKCPSCLWPKSADGTVRVPYRLSADYSDNEKSSIRDAL--LEFNTLTCVHFVERSTEEDFLDIVSDS-GCWSSIGRTGG--AQTLSLMSSGCL-AKGIIQHEVDHALGFYHEQSRSDRDTYIDVLWQYICESDWGSFEMVDTDNLDLPYDYSSVMHYGWYAFSNTSGQPSLRPKPDPTANIGQRYGLSPLDVSKVKELYGC 258

[Close]

BLAST of Ast1 protein vs. Ensembl Xenopus
Match: ENSXETT00000022029.1 (astacin-like metalloendopeptidase [Source:NCBI gene;Acc:100496745])

HSP 1 Score: 94.3597 bits (233), Expect = 1.687e-21
Identity = 66/222 (29.73%), Postives = 106/222 (47.75%), Query Frame = 2

Query:  107 MKMGDMLLDKSDIALINGFKNHYANTKKWPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKF-TESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRK--DGGAIGYNHDTTPNDLNMIRNFYGC 748
            M  GD+ + KS  AL+   K     +  WP +      V Y+ + N+   +  ++R  + E+   +  C++F T +N+  Y+ +    +GC+S +G   G   Q ++L   GC++  G ++HE +H+LGF H   R DRDN+I INW  I   +   F K +   L  PY+  SVMHY    FA +  +  +  K      IG     +  D+  I   Y C
Sbjct:    1 MIQGDIAVKKSRNALMCPGK-----SCLWPRSRKGLVLVPYTLSNNYNSTERDIIRAAMDEVT--VLTCIQFVTYNNESDYLRIR-PYDGCWSYIGRVGG--AQDVSLMKTGCLH-HGVIQHELLHSLGFQHEQCRSDRDNYININWDNISHDKERNFLKMNTQNLGSPYDYLSVMHYGKFAFATNSGKPTLEPKGNPSAMIGQRVGLSSLDVEKINRLYQC 211

[Close]

BLAST of Ast1 protein vs. Ensembl Xenopus
Match: kpna4 (karyopherin alpha 4 (importin alpha 3) [Source:Xenbase;Acc:XB-GENE-1011058])

HSP 1 Score: 92.4337 bits (228), Expect = 7.884e-21
Identity = 69/232 (29.74%), Postives = 115/232 (49.57%), Query Frame = 2

Query:  116 GDMLLDKSDIALINGFKNHYANTKKWPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA--IGYNHDTTPNDLNMIRNFYGC*RR*KTNICKICL 787
            G+M L +    LIN  ++  ++   WP +      V Y F+ N+  D++ + +  + E +     CV+F  ++ Q  ++ + S   GC S++G   G   Q + L + GCM S+G ++HE  HALGF H HMR DRD+++TI    I  S  + F+K     +   Y+  SVMHY+   F+  P +  ++ K   +  IG     +  D+  I+  Y C      ++C I L
Sbjct:   49 GNMALTEGGDILINIGRSATSSDYLWPKSADGTVVVPYIFSYNYSADELTLFKTAMQEFETL--TCVRFVPKTIQRDFLNIVSNG-GCLSMVGRNGG--GQKVELASYGCM-SRGVIQHELNHALGFYHEHMRSDRDDYVTIITENIIPSYENYFSKRKTNNMGIIYDYNSVMHYSRNTFSISPDKSTIVPKPDPSIPIGQRDGLSILDILKIKKLYQC------DVCAILL 268

[Close]

BLAST of Ast1 protein vs. Ensembl Xenopus
Match: ENSXETT00000032191.1 (astacin-like metallo-endopeptidase 3 gene b1 [Source:NCBI gene;Acc:100498420])

HSP 1 Score: 90.5077 bits (223), Expect = 4.023e-20
Identity = 59/193 (30.57%), Postives = 97/193 (50.26%), Query Frame = 2

Query:  191 WPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 748
            WP +      V Y+F+ N+  D++ + +  + E +     CV+F   +N+  ++ + S   GC S LG   G   Q + L++ GC+Y +G ++HE  HALGF H   R DRD+++TI+   I       F K D   L   Y+ +SVMHY+ + F+ +    +V + D    IG     +  D++ I   Y C
Sbjct:  124 WPKSTDGTVIVPYNFSSNYSADQLALFKSTMQEYES--LTCVRFVPRANETDFLSIVS-DNGCASFLGKVGG--DQTVQLDSYGCIY-RGIIQHELNHALGFYHEQSRSDRDDYVTIHTENIIPGYEGNFNKADSNNLGLEYDYSSVMHYSGDAFSKNGNLTIVPKPDPTVPIGQRDGLSILDVSKINRLYQC 310

[Close]

BLAST of Ast1 protein vs. Ensembl Xenopus
Match: ENSXETT00000032173.1 (astacin-like metallo-endopeptidase 3 gene b1 [Source:NCBI gene;Acc:100498420])

HSP 1 Score: 90.5077 bits (223), Expect = 4.061e-20
Identity = 59/193 (30.57%), Postives = 97/193 (50.26%), Query Frame = 2

Query:  191 WPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 748
            WP +      V Y+F+ N+  D++ + +  + E +     CV+F   +N+  ++ + S   GC S LG   G   Q + L++ GC+Y +G ++HE  HALGF H   R DRD+++TI+   I       F K D   L   Y+ +SVMHY+ + F+ +    +V + D    IG     +  D++ I   Y C
Sbjct:  124 WPKSTDGTVIVPYNFSSNYSADQLALFKSTMQEYES--LTCVRFVPRANETDFLSIVS-DNGCASFLGKVGG--DQTVQLDSYGCIY-RGIIQHELNHALGFYHEQSRSDRDDYVTIHTENIIPGYEGNFNKADSNNLGLEYDYSSVMHYSGDAFSKNGNLTIVPKPDPTVPIGQRDGLSILDVSKINRLYQC 310

[Close]

BLAST of Ast1 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 79.7221 bits (195), Expect = 1.525e-16
Identity = 58/218 (26.61%), Postives = 94/218 (43.12%), Query Frame = 2

Query:  125 LLDKSDIALINGFKNHYANTKKWPNN-----NVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMY-SKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFYGC*R 754
             L + DI   + F+       KWP        + +  +  +      V+     E +R    C++F     Q  ++ +     GC+S +G + GM  Q ++L  P C+   +G V HE +H LGF H H R DRD +I +NW  I       F K     +  PY+ +SVMHY    F+   +  ++ L      IG   + + +D+  +   Y C R
Sbjct:   72 FLVEGDIIRPSPFRLLSVTNNKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRFVAYHGQRDFVSIL-PMAGCFSGVGRSGGM--QVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQPTIIPLWTSSVHIGQRWNLSTSDITRVCRLYNCSR 283

[Close]

BLAST of Ast1 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 78.5666 bits (192), Expect = 2.848e-16
Identity = 58/218 (26.61%), Postives = 94/218 (43.12%), Query Frame = 2

Query:  125 LLDKSDIALINGFKNHYANTKKWPNN-----NVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMY-SKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFYGC*R 754
             L + DI   + F+       KWP        + +  +  +      V+     E +R    C++F     Q  ++ +     GC+S +G + GM  Q ++L  P C+   +G V HE +H LGF H H R DRD +I +NW  I       F K     +  PY+ +SVMHY    F+   +  ++ L      IG   + + +D+  +   Y C R
Sbjct:   51 FLVEGDIIRPSPFRLLSVTNNKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRFVAYHGQRDFVSI-LPMAGCFSGVGRSGGM--QVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQPTIIPLWTSSVHIGQRWNLSTSDITRVCRLYNCSR 262

[Close]

Query:  125 LLDKSDIALINGFKNHYANTKKWPNN-----NVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMY-SKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFYGC*R 754
             L + DI   + F+       KWP        + +  +  +      V+     E +R    C++F     Q  ++ +     GC+S +G + GM  Q ++L  P C+   +G V HE +H LGF H H R DRD +I +NW  I       F K     +  PY+ +SVMHY    F+   +  ++ L      IG   + + +D+  +   Y C R
Sbjct:   51 FLVEGDIIRPSPFRLLSVTNNKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRFVAYHGQRDFVSI-LPMAGCFSGVGRSGGM--QVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQPTIIPLWTSSVHIGQRWNLSTSDITRVCRLYNCSR 262

[Close]

BLAST of Ast1 protein vs. Ensembl Mouse
Match: Mep1b (meprin 1 beta [Source:MGI Symbol;Acc:MGI:96964])

HSP 1 Score: 77.0258 bits (188), Expect = 1.502e-15
Identity = 69/216 (31.94%), Postives = 101/216 (46.76%), Query Frame = 2

Query:  128 LDKSDIAL-INGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQR-DLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLG-YAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGC---ELDGPYETTSVMHYNSEGFACDPREKVVLR--KDGGAIGYNHDTTPNDLNMIRNFYGC 748
            L + DI L  NG  +   + K+WP+  + Y    +   +   V+   L   +R  L  C+ F   S + +YI V  K  GC+S +G   AG   Q L++    C     TV+HEF+HALGF H   R DRD+++ I W RI+  +   F   +      L+ PY+ TSVMHY+   F       +V R  +    IG   D +  DL  +   Y C
Sbjct:   50 LFEGDIKLEANGKNSIIGDHKRWPHT-IPYVLEDSLEMNAKGVI---LNAFERYRLKTCIDFKPWSGEANYISV-FKGSGCWSSVGNIHAG--KQELSI-GTNCD-RIATVQHEFLHALGFWHEQSRADRDDYVIIVWDRIQPGKEHNFNIYNDSVSDSLNVPYDYTSVMHYSKTAFQNGTESTIVTRISEFEDVIGQRMDFSDYDLLKLNQLYNC 256

[Close]

BLAST of Ast1 protein vs. Ensembl Mouse
Match: Tll1 (tolloid-like [Source:MGI Symbol;Acc:MGI:106923])

HSP 1 Score: 77.0258 bits (188), Expect = 1.786e-15
Identity = 60/195 (30.77%), Postives = 87/195 (44.62%), Query Frame = 2

Query:  191 WPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDG---PYETTSVMHYNSEGFACDPREKVVL--RKDGG---AIGYNHDTTPNDLNMIRNFYGC 748
            WP   + Y   GNF   +  + +Q +   ++    CV FTE S++  YI    +  GC S +G     P Q +++      +  G V HE  H +GF H H R DRDNH+TI    I+  Q   F K +  E++     Y+  S+MHY    F+       +L  R D G   AIG     +  D+   R  Y C
Sbjct:  157 WPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKH--TCVTFTERSDEESYIVFTYRPCGCCSYVGRRGNGP-QAISIGKNCDKF--GIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRC 346

[Close]

BLAST of Ast1 protein vs. UniProt/SwissProt
Match: sp|P55112|NAS4_CAEEL (Zinc metalloproteinase nas-4 OS=Caenorhabditis elegans OX=6239 GN=nas-4 PE=2 SV=4)

HSP 1 Score: 98.2117 bits (243), Expect = 9.075e-23
Identity = 61/195 (31.28%), Postives = 97/195 (49.74%), Query Frame = 2

Query:  185 KKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE---SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCD---GCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 748
            ++WPNN + Y+ +  +      V+   + E       CVKF     S    Y+ ++   EGCYSL+G   G   QP++L++ GC+   GT+ HE +HA+GF H   R+DRD++I + W  +      QF K +      LD PY+  S+MHY    F+   ++ +V +K G   +G     +  D+  I   Y C
Sbjct:  102 RRWPNNEIPYTLSSQYGSYARSVIANAMNEYH--TKTCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGG--KQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289

[Close]

BLAST of Ast1 protein vs. UniProt/SwissProt
Match: sp|P31579|LCE_ORYLA (Low choriolytic enzyme OS=Oryzias latipes OX=8090 GN=lce PE=1 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 2.155e-22
Identity = 66/226 (29.20%), Postives = 105/226 (46.46%), Query Frame = 2

Query:   95 SVDSMKMGDMLLDKSDIALINGFKNHYA-NTKKWPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            S++ +  GD++L K+     N  K   A ++ +WP ++     V Y  + N+  D+   +R  + E       C+ F   +N+  Y+ +  +  GC S++GY      Q + L+  GC+     ++HE +HALGF H H R DRD H+ INW  I       F K D   L  PY+  S+MHY    F  D +E +  +     AIG     +  D+  +   Y C
Sbjct:   56 SMEELLEGDLVLPKTR----NAMKCFGAPDSCRWPKSSNGIVKVPYVVSDNYESDEKETIRNAMKEFAE--KTCIHFVPRNNERAYLSLEPRF-GCKSMMGYVGD--KQVVVLQRFGCI-KHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDYGSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC 271

[Close]

BLAST of Ast1 protein vs. UniProt/SwissProt
Match: sp|P31581|HCE2_ORYLA (High choriolytic enzyme 2 OS=Oryzias latipes OX=8090 GN=hceb PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.057e-21
Identity = 57/155 (36.77%), Postives = 81/155 (52.26%), Query Frame = 2

Query:  185 KKWPNNNVV--YSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFA 640
            KK  N  VV  Y  +  + R ++  +   +         C++F   +N+  +I V SK  GCYS LG   G   Q L+L   GCMYS G ++HE  HALGF H   R DRD+++ INW  I  +    F K D   L+ PY+ +S+MHY  + F+
Sbjct:   92 KKASNGFVVIPYVISSQYSRGEVATIEGAMRAFNGRT--CIRFVRRTNEYDFISVVSKN-GCYSELGRKGGQ--QELSLNRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFS 240

[Close]

BLAST of Ast1 protein vs. UniProt/SwissProt
Match: sp|P31580|HCE1_ORYLA (High choriolytic enzyme 1 OS=Oryzias latipes OX=8090 GN=hcea PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 3.515e-21
Identity = 49/116 (42.24%), Postives = 68/116 (58.62%), Query Frame = 2

Query:  296 CVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFA 640
            C++F   +N+  +I V SK  GCYS LG   G   Q L++   GCMYS G ++HE  HALGF H   R DRD+++ INW  I  +    F K D   L+ PY+ +S+MHY  + F+
Sbjct:  120 CIRFVRRTNEYDFISVVSKT-GCYSELGRKGGQ--QELSINRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWENIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFS 231

[Close]

BLAST of Ast1 protein vs. UniProt/SwissProt
Match: sp|P55113|NAS7_CAEEL (Zinc metalloproteinase nas-7 OS=Caenorhabditis elegans OX=6239 GN=nas-7 PE=2 SV=2)

HSP 1 Score: 93.9745 bits (232), Expect = 6.486e-21
Identity = 66/232 (28.45%), Postives = 112/232 (48.28%), Query Frame = 2

Query:   74 RQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYAN--TKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGH--YIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELD---GPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            R++L  + +    + D    KSDI L    K +  +   K WPN  + Y+ + ++   +  ++ + + +       C++F     G   Y+ +  K +GC+S +G  +G+  Q L+L+N GCM    T+ HE +H +GF H H R DRDN I I W  I+     QF K D  +      PY+  S++HY+S  F+ +    ++ +     IG   D +  D++ I   Y C
Sbjct:   51 REELFGKHIPVEVVNDF---KSDIRLPRRHKRNGVSRAAKLWPNARIPYAISPHYSPHERALLAKAVKQYHE--KTCIRFVPRQTGEPDYLFIG-KVDGCFSEVGRTSGV--QVLSLDN-GCM-EYATIIHEMMHVVGFYHEHERWDRDNFIDIIWQNIDRGALDQFGKVDLSKTSYYGQPYDYKSILHYDSLAFSKNGFPTMLPKVKSATIGNARDFSDVDISKINRMYNC 272

[Close]

BLAST of Ast1 protein vs. TrEMBL
Match: A0A060Q785 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast1 PE=2 SV=1)

HSP 1 Score: 527.709 bits (1358), Expect = 0.000e+0
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 2

Query:    2 MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC
Sbjct:    1 MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 249

[Close]

BLAST of Ast1 protein vs. TrEMBL
Match: A0A060Q714 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast2 PE=2 SV=1)

HSP 1 Score: 410.223 bits (1053), Expect = 5.773e-143
Identity = 191/251 (76.10%), Postives = 214/251 (85.26%), Query Frame = 2

Query:    2 MQKMFLMCLAIAVIGYVSGQCGCPRQ--KLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            M+K+ L+ L   VI Y +GQCGCPRQ  KL  R +D++KMGDMLLD +DI +INGFKNHYAN+KKWPNN V Y+FAGNFP+DKI  VRQ LGELQ+DL NCVKF E   GHYIEVNSK+EGCYS+LG   G P+QPLNLENPGCMYSKGT+KHEFIHALGFMHTHMRKDRDNHITI W RI TSQCSQFTKCDGCE DGPYET SVMHYNSEGFAC+P EKVV++K+GGAIGYNH+TT NDL MI+NFY C
Sbjct:    1 MEKLILLLLGFVVIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGG-PSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQMIKNFYNC 250

[Close]

BLAST of Ast1 protein vs. TrEMBL
Match: A0A060Q786 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast6 PE=2 SV=1)

HSP 1 Score: 392.119 bits (1006), Expect = 9.484e-136
Identity = 181/249 (72.69%), Postives = 207/249 (83.13%), Query Frame = 2

Query:    2 MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            M+ +F + L +A + YVSGQCGCPRQKLLKR +DSM+MGDM+LDKSDIALINGFK HY+NTK+WPNN V++SFA  FP DK  +VR+CL ELQ+DLGNCVKF+ES   HYIEV+S  +GCYSLLGY  G P QPLNL+NPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITI W RI TS CSQF KC+GC+LDGPYET SVMHY S GFAC P E VV ++DGG I YNH T+ NDL+M+R FY C
Sbjct:    1 MRSLFCLLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGG-PNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 248

[Close]

BLAST of Ast1 protein vs. TrEMBL
Match: I1ZIC3 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast3 PE=2 SV=1)

HSP 1 Score: 297.36 bits (760), Expect = 2.467e-98
Identity = 136/249 (54.62%), Postives = 184/249 (73.90%), Query Frame = 2

Query:    2 MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            MQK FL+ + + +   V+ QCGC ++KL+ R+ +++ +GDMLL K+DIA+INGFK+H    + WP   V ++FA +FP DK  +V+QCL ELQ DLGNCV+F ES QG +I V+++ +GCY+++G   G   Q LNL+NP CM SKGT+KHEFIHALGFMHTHMR DRDN I+INW  I++SQC  F KC+GC +DG YET SVMHY+S+ F C      +++K+G  I YNH+TTPNDL+MI++ Y C
Sbjct:    5 MQK-FLILIVLVLANEVASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIG-CTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNC 251

[Close]

BLAST of Ast1 protein vs. TrEMBL
Match: A0A060Q750 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast5 PE=2 SV=1)

HSP 1 Score: 229.565 bits (584), Expect = 1.356e-71
Identity = 113/253 (44.66%), Postives = 156/253 (61.66%), Query Frame = 2

Query:    2 MQKMFLMCLAIAVIGYV---SGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMP-TQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            M ++F+    ++++  V   +G+CGC + KL KR++ S+K+ DMLLD  D+  I GF+ +  N ++W +N + Y+F   FP DK  +VR+ + EL  DL  CVK+ ES  G++I+V S Q GC+S +G       +Q LNLENPGC+YSKGT+KHEFIHA GFMHT MR DRD  + I W RI++  C  F  C  CE    Y+T SVMHY SE F C  +   ++ K    I YNH T   D  MI++ Y C
Sbjct:    1 MNQIFIGLFLLSILNLVFIEAGKCGCQKSKL-KRAI-SLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYTFHPQFPADKKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQC 251

[Close]

BLAST of Ast1 protein vs. Ensembl Cavefish
Match: c6ast1 (low choriolytic enzyme-like [Source:NCBI gene;Acc:111191947])

HSP 1 Score: 102.834 bits (255), Expect = 8.697e-26
Identity = 69/225 (30.67%), Postives = 115/225 (51.11%), Query Frame = 2

Query:  104 SMKMGDMLLDKSDIALINGFKNHYANTKK-----WPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGH-YIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFY 742
            +++ GD+L+D       +G +N    T +     WP N+     + Y  +  F  ++  ++   L   ++    C++F   ++   YI+V S  EGCYS +G   G   Q L+L++ GC+Y K  ++HEF+HALGF H   R DRD HI I +  I+  +   F K D   L+ PY+  SVMHY  + F+ + +  +V +     A+G   + + ND+  I+  Y
Sbjct:   54 TIRFGDILVD-------DGLQNADRCTSRKPSCMWPMNSDGNTYIPYIISNQFSPNEKDLITSVLKTFEK--STCIRFKPRDREEDYIDVQS-LEGCYSFVGRTGGR--QTLSLKSRGCLYKK-IIQHEFLHALGFHHEQCRSDRDKHIRILYENIDPGKEHNFDKKDTNNLNTPYDYGSVMHYGRDAFSRNGQPTMVPIPNQNVAVGRAEEMSANDILRIKKLY 265

[Close]

BLAST of Ast1 protein vs. Ensembl Cavefish
Match: hce2l1 (high choriolytic enzyme 1-like [Source:NCBI gene;Acc:103038207])

HSP 1 Score: 103.219 bits (256), Expect = 8.882e-26
Identity = 64/191 (33.51%), Postives = 97/191 (50.79%), Query Frame = 2

Query:  191 WP---NNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGG-AIGYNHDTTPNDLNMIRNFYGC 748
            WP   +  V   +A +   D +  +   +G L     +CVKF   ++Q +++++  K  GC+S LG   G  TQ L+L+ PGCM+S G   HE +HALGF+H   R DRD ++TI W  I   Q   F   D   L+  Y+ +SVMHY    F+ +    ++ + D    IG     +P D++ I   Y C
Sbjct:   90 WPKSVDGFVYVPYALSMQYDTMDRITIEMGMLDISSKSCVKFVPRTHQANFLDIQPK-FGCWSYLGMVGG--TQALSLQTPGCMWS-GVASHELMHALGFVHEQSRSDRDRYVTILWDNIIEGQKHNFRMYDTNNLNTAYDYSSVMHYGRFAFSEEGLPTIIPKPDPNIPIGQREGPSPLDIHKINLLYNC 276

[Close]

BLAST of Ast1 protein vs. Ensembl Cavefish
Match: ENSAMXT00000033223.1 (high choriolytic enzyme 1-like [Source:NCBI gene;Acc:111197481])

HSP 1 Score: 102.449 bits (254), Expect = 1.037e-25
Identity = 64/213 (30.05%), Postives = 101/213 (47.42%), Query Frame = 2

Query:  116 GDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 748
            GD++L ++  AL+    N +          V ++ + +F      V+   +         C++F + S +  Y+ + ++ +GCYS LG   G   Q ++L   GC+Y  G V+HE  HALGF H H R DRD ++TINW  I+ +    F K +   L+ PY+ +SVMHY    F  +    +    D    IG   D +  D+  I   Y C
Sbjct:   49 GDIVLPRTRNALVCANNNCFWKKSSTGLVQVPFTVSADFSSTDRAVIAGAMATFHSK--TCIRFVDRSTEIDYLSIENR-DGCYSSLGRTGG--KQVVSLSTRGCVY-HGIVQHELNHALGFYHEHTRSDRDRYVTINWQNIDPTMQYNFNKENTNNLNTPYDYSSVMHYGKTAFTINGLNTITPIPDASVQIGQRVDLSVIDIQRINTLYNC 255

[Close]

BLAST of Ast1 protein vs. Ensembl Cavefish
Match: ENSAMXT00000056363.1 (high choriolytic enzyme 1-like [Source:NCBI gene;Acc:111197481])

HSP 1 Score: 102.449 bits (254), Expect = 1.037e-25
Identity = 64/213 (30.05%), Postives = 101/213 (47.42%), Query Frame = 2

Query:  116 GDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 748
            GD++L ++  AL+    N +          V ++ + +F      V+   +         C++F + S +  Y+ + ++ +GCYS LG   G   Q ++L   GC+Y  G V+HE  HALGF H H R DRD ++TINW  I+ +    F K +   L+ PY+ +SVMHY    F  +    +    D    IG   D +  D+  I   Y C
Sbjct:   49 GDIVLPRTRNALVCANNNCFWKKSSTGLVQVPFTVSADFSSTDRAVIAGAMATFHSK--TCIRFVDRSTEIDYLSIENR-DGCYSSLGRTGG--KQVVSLSTRGCVY-HGIVQHELNHALGFYHEHTRSDRDRYVTINWQNIDPTMQYNFNKENTNNLNTPYDYSSVMHYGKTAFTINGLNTITPIPDASVQIGQRVDLSVIDIQRINTLYNC 255

[Close]

BLAST of Ast1 protein vs. Ensembl Cavefish
Match: ENSAMXT00000044782.1 (high choriolytic enzyme 1-like [Source:NCBI gene;Acc:111197481])

HSP 1 Score: 102.449 bits (254), Expect = 1.037e-25
Identity = 64/213 (30.05%), Postives = 101/213 (47.42%), Query Frame = 2

Query:  116 GDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 748
            GD++L ++  AL+    N +          V ++ + +F      V+   +         C++F + S +  Y+ + ++ +GCYS LG   G   Q ++L   GC+Y  G V+HE  HALGF H H R DRD ++TINW  I+ +    F K +   L+ PY+ +SVMHY    F  +    +    D    IG   D +  D+  I   Y C
Sbjct:   49 GDIVLPRTRNALVCANNNCFWKKSSTGLVQVPFTVSADFSSTDRAVIAGAMATFHSK--TCIRFVDRSTEIDYLSIENR-DGCYSSLGRTGG--KQVVSLSTRGCVY-HGIVQHELNHALGFYHEHTRSDRDRYVTINWQNIDPTMQYNFNKENTNNLNTPYDYSSVMHYGKTAFTINGLNTITPIPDASVQIGQRVDLSVIDIQRINTLYNC 255

[Close]

BLAST of Ast1 protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007253.1 (pep scaffold:Pmarinus_7.0:GL476910:54360:144194:-1 gene:ENSPMAG00000006538.1 transcript:ENSPMAT00000007253.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 77.7962 bits (190), Expect = 1.301e-16
Identity = 60/195 (30.77%), Postives = 88/195 (45.13%), Query Frame = 2

Query:  191 WPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDG---PYETTSVMHYNSEGFACDPREKVVLRK--DGG---AIGYNHDTTPNDLNMIRNFYGC 748
            WP   + Y   GNF   +  + RQ +   ++    CV F E +++  YI    +  GC S +G   G P Q +++      +  G V HE  H +GF H H R DRD+H+TI  A I+  Q   F K +  E+D     Y+  S+MHY    F+       +L +  D G   AIG     +  D+   R  Y C
Sbjct:   22 WPGGVIPYIVGGNFTGSQKAMFRQAMRHWEKH--TCVTFIERTDEESYIVFTYRPCGCCSYVGRRGGGP-QAISIGKNCDKF--GIVVHELGHVIGFWHEHTRPDRDDHVTIIKANIQPGQEYNFLKMEPGEVDSLGESYDFDSIMHYARNTFSRGIFLDTILPRPDDSGVRPAIGQRTRLSQGDIAQARKLYRC 211

[Close]

BLAST of Ast1 protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004913.1 (pep scaffold:Pmarinus_7.0:GL478359:22377:26907:1 gene:ENSPMAG00000004462.1 transcript:ENSPMAT00000004913.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.6842 bits (130), Expect = 1.585e-9
Identity = 28/74 (37.84%), Postives = 40/74 (54.05%), Query Frame = 2

Query:  440 GTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDG-PYETTSVMHYNSEGFACDPREK 658
            GTV HE  HA+G++H   R DRD  I++    ++  Q  QF      +  G PY+  SVMHY+    AC  R++
Sbjct:   25 GTVAHELGHAVGYVHEQSRPDRDAFISVLEGNVQDGQLPQFAVYSATDPRGVPYDLKSVMHYSRNVRACGTRDR 98

[Close]

BLAST of Ast1 protein vs. Ensembl Nematostella
Match: EDO42421 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1T1])

HSP 1 Score: 92.8189 bits (229), Expect = 4.457e-23
Identity = 60/193 (31.09%), Postives = 91/193 (47.15%), Query Frame = 2

Query:  185 KKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE--SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDG---PYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            K WP   VVY     F R    + +        +   C++F +  +N   Y+ +  +  GC S +G   G   Q L+L + GC + KGT+ HE  HALGF H   R DRD+++TI +  I     S F K    E+D    PY+  S+MHY  + F+ + +  +V +K G   G     +P D+  +   Y C
Sbjct:   10 KLWPGGVVVYELDPRFERSSRAMEKLTQAISDYEKYTCIRFRKKTANDNDYVYIKDRG-GCRSHVGRKGG--KQYLSLSS-GC-WHKGTIIHELGHALGFFHEQTRPDRDDYVTILFENIRLGMASNFMKHSAGEVDSLGTPYDYGSIMHYGPKIFSANGKPTIVAKKPGVKFGQRDGLSPIDIKQVNLLYKC 197

[Close]

BLAST of Ast1 protein vs. Ensembl Nematostella
Match: EDO34721 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNJ4])

HSP 1 Score: 92.4337 bits (228), Expect = 2.106e-22
Identity = 65/217 (29.95%), Postives = 105/217 (48.39%), Query Frame = 2

Query:  104 SMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELD---GPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFY 742
            + ++ D L+  S  AL++   +       WPN  + Y F G          R  + E + +   C++F E +++  YI V S + GC+S +G + G   Q L+L        KGT  HE +H LGF H   R+DRDN+ITI+W  I++ +   F K +  ++D    PY+  S+MHY    F+ + R  +V +    +IG     +  DL  +   Y
Sbjct:   12 TTRIMDQLMSPSREALVDPVSH-------WPNGRLPYKF-GAVSNSVKEAFRAAIDEYEAN--TCIRFVERTDETDYILVVS-EGGCWSPIGRSGGQ--QKLSLGT--YCDRKGTAMHELMHTLGFFHEQSRRDRDNYITIHWDNIQSDKQFNFQKYEHGKVDTLGAPYDYGSIMHYPMFAFSSNGRATIVPKDPTASIGQRSGFSKVDLQQLNKLY 213

[Close]

BLAST of Ast1 protein vs. Ensembl Nematostella
Match: EDO37770 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEU5])

HSP 1 Score: 92.8189 bits (229), Expect = 3.662e-22
Identity = 75/262 (28.63%), Postives = 120/262 (45.80%), Query Frame = 2

Query:    2 MQKMFLMCL-AIAVIGYVS--GQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALIN-----GFKNHYANTKKWPNNNVVYSFAGNFPRDKIP-VVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELD---GPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            M+   L+C  AI+++   S     G PR  L +        GDM+L       +N       +    + KKWPN  + Y    +        V++  + E +     CV+F + + Q +Y+    K +GC+S +G       Q ++L   GC + KGT  HE  HALGF H   R DRDN++T++W  I+T     F K     ++    PY+  S+MHY +  F+ + R  +  +K G  IG     +  D++ +   YGC
Sbjct:    1 MRLSVLLCFTAISLVSSASIDENAGNPRSDLFE--------GDMILTPDQQQAVNNGDLGASRGAPIHDKKWPNAILPYEIRSDLKGTPAEGVIQNAMREWESK--TCVRFQKRTTQKNYVYF-FKGDGCWSYVG-MLDRGAQAISL-GSGC-WHKGTAAHEIGHALGFYHEQSRPDRDNYVTVHWDNIKTDSKHNFNKYSADRINTFGTPYDFGSLMHYGTNYFSVNGRATITPKKSGAKIGQRDYLSDLDVHQMNLRYGC 248

[Close]

BLAST of Ast1 protein vs. Ensembl Nematostella
Match: EDO29086 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T4M8])

HSP 1 Score: 85.1149 bits (209), Expect = 5.504e-21
Identity = 47/131 (35.88%), Postives = 67/131 (51.15%), Query Frame = 2

Query:  359 CYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTK-CDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            CYS +G+      Q +++   GC    G V HE +HALGF H   RKDRD ++TINW  I   +   F K  +   L  PY+  S+MHY    F+ + R+ +  +K G AIG     +  D+  +R  Y C
Sbjct:    1 CYSYVGHQ--RREQDISI-GRGCE-RMGIVAHEILHALGFWHEQSRKDRDQYVTINWNNIRAGKAHNFNKHLNSHTLGAPYDYNSLMHYGRRSFSKNGRDTITPKKSGAAIGQRDGLSRVDIWQVRKRYSC 127

[Close]

BLAST of Ast1 protein vs. Ensembl Nematostella
Match: EDO41420 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4I5])

HSP 1 Score: 85.1149 bits (209), Expect = 6.030e-20
Identity = 54/192 (28.12%), Postives = 86/192 (44.79%), Query Frame = 2

Query:  182 TKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDG---PYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            T +WP   + Y+   +     +  +R  + ++  D   C++F E    +      +  GC S++G       Q ++L   GC Y  GTV HE  HALGF H   R DRDN++TI+W  I  +    F       +D    PY+  SVMHY    F+ + R  +  ++ G  +G     +  D+  +   Y C
Sbjct:   38 TGRWPEGVMAYTIDRSLSNRAMNAIRAGM-KMWTD-NTCIRFKERTNEYAYARFWRGRGCSSMVGRTG--RRQDISLAG-GCWY-PGTVAHEIGHALGFYHEQSRPDRDNYVTIHWNNISPNMKFNFNMYPRSTIDSLGTPYDYRSVMHYQGNAFSSNGRATITAKQSGVTLGNRRQLSKVDIQQMNLMYKC 223

[Close]

BLAST of Ast1 protein vs. Ensembl Medaka
Match: mc6ast5 (astacin like metallo-protease [Source:NCBI gene;Acc:100049509])

HSP 1 Score: 107.071 bits (266), Expect = 2.965e-27
Identity = 58/155 (37.42%), Postives = 85/155 (54.84%), Query Frame = 2

Query:  290 GNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGG-AIGYNHDTTPNDLNMIRNFYGC 748
            G C+KF   +++ H++++  +  GC+S LG   G  +Q L+L+ PGCM+S G   HEF+HALGF+H   R DRD+++TI W  I       F K     L+ PY+ +SVMHY    F+ D    ++ R D    IG     +  DL+ I   Y C
Sbjct:  129 GTCIKFVPRTHEAHFLDIQPRY-GCWSFLGQTGG--SQTLSLQTPGCMWS-GIAAHEFMHALGFVHEQSRSDRDHYVTIVWKNIMPDHAHNFRKQATNNLNSPYDYSSVMHYGRYAFSGDGGPTIIPRPDPYIPIGQRDGPSALDLHKINVLYDC 279

[Close]

BLAST of Ast1 protein vs. Ensembl Medaka
Match: mc6ast4 (astacin like metallo-protease [Source:NCBI gene;Acc:100049317])

HSP 1 Score: 103.605 bits (257), Expect = 5.708e-26
Identity = 64/220 (29.09%), Postives = 111/220 (50.45%), Query Frame = 2

Query:  116 GDMLLDKSDIALINGFKNH--YANTKKWPNN-----NVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGG-AIGYNHDTTPNDLNMIRNFYGC 748
            G   L + DIA+  G ++   +A +  W  +      + Y+ + ++    + ++++ +  ++   G CV+F  +++Q  Y+++  K  GC+S LG   G   Q L+L+NP CM   G + HEF+HALGF+H   R DRDN+IT+ W  I   +   F K     LD PY+  S+MH+    ++ D +  +V + +    +G     +  D   I   Y C
Sbjct:   62 GTTTLREGDIAVSTGRRSKMCFARSCLWSKSVDGHVYIAYTISPDYTEADVKLIKKGMENIEE--GTCVRFVPQTHQRDYVDIQPK-SGCWSYLGARGG--RQTLSLQNPDCM-QVGVISHEFMHALGFVHEQSRFDRDNYITVMWPNIWRDRLRNFEKFKTDSLDLPYDYGSIMHFGMYAYSMDGQPTLVAKNNRNMKLGQASSLSHTDKLKINRLYQC 275

[Close]

BLAST of Ast1 protein vs. Ensembl Medaka
Match: lce (protease [Source:NCBI gene;Acc:100049451])

HSP 1 Score: 96.6709 bits (239), Expect = 1.838e-23
Identity = 66/226 (29.20%), Postives = 105/226 (46.46%), Query Frame = 2

Query:   95 SVDSMKMGDMLLDKSDIALINGFKNHYA-NTKKWPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            S++ +  GD++L K+     N  K   A ++ +WP ++     V Y  + N+  D+   +R  + E       C+ F   +N+  Y+ +  +  GC S++GY      Q + L+  GC+     ++HE +HALGF H H R DRD H+ INW  I       F K D   L  PY+  S+MHY    F  D +E +  +     AIG     +  D+  +   Y C
Sbjct:   56 SMEELLEGDLVLPKTR----NAMKCFGAPDSCRWPKSSNGIVKVPYVVSDNYESDEKETIRNAMKEFAE--KTCIHFVPRNNERAYLSLEPRF-GCKSMMGYVGD--KQVVVLQRFGCI-KHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDYGSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC 271

[Close]

BLAST of Ast1 protein vs. Ensembl Medaka
Match: lce (protease [Source:NCBI gene;Acc:100049451])

HSP 1 Score: 96.2857 bits (238), Expect = 2.404e-23
Identity = 66/226 (29.20%), Postives = 105/226 (46.46%), Query Frame = 2

Query:   95 SVDSMKMGDMLLDKSDIALINGFKNHYA-NTKKWPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            S++ +  GD++L K+     N  K   A ++ +WP ++     V Y  + N+  D+   +R  + E       C+ F   +N+  Y+ +  +  GC S++GY      Q + L+  GC+     ++HE +HALGF H H R DRD H+ INW  I       F K D   L  PY+  S+MHY    F  D +E +  +     AIG     +  D+  +   Y C
Sbjct:   56 SMEELLEGDLVLPKTR----NAMKCFGAPDSCRWPKSSNGIVKVPYVVSDNYESDEKETIRNAMKEFAE--KTCIHFVPRNNERAYLSLEPRF-GCKSMMGYVGD--KQVVVLQRFGCI-KHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDYGSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC 271

[Close]

BLAST of Ast1 protein vs. Ensembl Medaka
Match: mc6ast3 (astacin like metallo-protease [Source:NCBI gene;Acc:100049508])

HSP 1 Score: 95.9005 bits (237), Expect = 3.390e-23
Identity = 70/254 (27.56%), Postives = 115/254 (45.28%), Query Frame = 2

Query:   20 MCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNH----YANTKKWPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 748
            + LA++   + + +     + L K + D +   D      DIA ++    +     +++  WP +      + Y  A +F   ++ V+ + L        +C++F   S Q  Y+ + S Q GCYS +G       Q L+L+  GC+Y   TV+HE +HALGF H   R DRD HI + W  I++     F K +   LD PY+  SVM Y+   F+ + R  +    D     G     + ND+  +   Y C
Sbjct:   14 ILLAVSTCSWATEKELSVSELLWKANKDVVHTEDEPFVVDDIAYVSKSDRNADQCTSSSCLWPKSADGRVYIPYVIANHFSSSELQVIERGLQSFSE--VSCIRFIPYSGQRDYVHIQS-QNGCYSYVGRIGY--GQTLSLDRQGCIY-HSTVQHELLHALGFNHEQCRSDRDQHIRVLWENIQSGLEYAFDKLNTLNLDTPYDYNSVMQYHRYAFSANNRPTMEPYPDPNVPFGTATQMSNNDITRLNRLYKC 261

[Close]

BLAST of Ast1 protein vs. Planmine SMEST
Match: SMESG000015174.1 (SMESG000015174.1)

HSP 1 Score: 527.709 bits (1358), Expect = 0.000e+0
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 2

Query:    2 MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC
Sbjct:    1 MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 249

[Close]

BLAST of Ast1 protein vs. Planmine SMEST
Match: SMESG000015174.1 (SMESG000015174.1)

HSP 1 Score: 521.931 bits (1343), Expect = 0.000e+0
Identity = 246/246 (100.00%), Postives = 246/246 (100.00%), Query Frame = 2

Query:   11 MFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            MFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC
Sbjct:    1 MFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 246

[Close]

BLAST of Ast1 protein vs. Planmine SMEST
Match: SMESG000015174.1 (SMESG000015174.1)

HSP 1 Score: 515.383 bits (1326), Expect = 0.000e+0
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 2

Query:   20 MCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            MCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC
Sbjct:    1 MCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 243

[Close]

BLAST of Ast1 protein vs. Planmine SMEST
Match: SMESG000072791.1 (SMESG000072791.1)

HSP 1 Score: 392.119 bits (1006), Expect = 2.637e-139
Identity = 181/249 (72.69%), Postives = 207/249 (83.13%), Query Frame = 2

Query:    2 MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            M+ +F + L +A + YVSGQCGCPRQKLLKR +DSM+MGDM+LDKSDIALINGFK HY+NTK+WPNN V++SFA  FP DK  +VR+CL ELQ+DLGNCVKF+ES   HYIEV+S  +GCYSLLGY  G P QPLNL+NPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITI W RI TS CSQF KC+GC+LDGPYET SVMHY S GFAC P E VV ++DGG I YNH T+ NDL+M+R FY C
Sbjct:    1 MRSLFCLLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGG-PNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 248

[Close]

BLAST of Ast1 protein vs. Planmine SMEST
Match: SMESG000072807.1 (SMESG000072807.1)

HSP 1 Score: 384.03 bits (985), Expect = 2.967e-136
Identity = 181/245 (73.88%), Postives = 202/245 (82.45%), Query Frame = 2

Query:   20 MCLAIAVIGYVSGQCGCPRQ--KLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 748
            M  +  +I Y      C  Q  KL  R +D++KMGDMLLD +DI +INGFKNHYAN+KKWPNN V Y+FAGNFP+DKI  VRQ LGELQ+DL NCVKF E   GHYIEVNSK+EGCYS+LG   G P+QPLNLENPGCMYSKGT+KHEFIHALGFMHTHMRKDRDNHITI W RI TSQCSQFTKCDGCE DGPYET SVMHYNSEGFAC+P EKVV++K+GGAIGYNH+TT NDL MI+NFY C
Sbjct:    1 MSTSSEIIYYYQASSFCLIQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGG-PSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQMIKNFYNC 244

The following BLAST results are available for this feature:

BLAST of Ast1 protein vs. Ensembl Human
BLAST of Ast1 protein vs. Ensembl Celegans
BLAST of Ast1 protein vs. Ensembl Fly
BLAST of Ast1 protein vs. Ensembl Zebrafish
BLAST of Ast1 protein vs. Ensembl Xenopus
BLAST of Ast1 protein vs. Ensembl Mouse
BLAST of Ast1 protein vs. UniProt/SwissProt
BLAST of Ast1 protein vs. TrEMBL
BLAST of Ast1 protein vs. Ensembl Cavefish
BLAST of Ast1 protein vs. Ensembl Sea Lamprey
BLAST of Ast1 protein vs. Ensembl Yeast
BLAST of Ast1 protein vs. Ensembl Nematostella
BLAST of Ast1 protein vs. Ensembl Medaka
BLAST of Ast1 protein vs. Planmine SMEST

BLAST of Ast1 protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ASTL	3.645e-20	29.17	astacin like metalloendopeptidase [Source:HGNC Sym...	[more]
TLL1	5.007e-15	29.95	tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843]	[more]
MEP1B	7.318e-15	30.28	meprin A subunit beta [Source:HGNC Symbol;Acc:HGNC...	[more]
MEP1B	7.594e-15	30.28	meprin A subunit beta [Source:HGNC Symbol;Acc:HGNC...	[more]
TLL1	1.416e-14	30.26	tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843]	[more]

BLAST of Ast1 protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
nas-4	7.134e-24	31.28	Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw...	[more]
nas-4	2.579e-23	31.28	Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw...	[more]
nas-4	2.770e-23	31.22	Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw...	[more]
nas-7	5.099e-22	28.45	Zinc metalloproteinase nas-7 [Source:UniProtKB/Sw...	[more]
nas-6	7.799e-21	31.40	Zinc metalloproteinase nas-6 [Source:UniProtKB/Sw...	[more]

BLAST of Ast1 protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CG6763	2.138e-33	31.16	gene:FBgn0039069 transcript:FBtr0084378	[more]
CG6763	2.138e-33	31.16	gene:FBgn0039069 transcript:FBtr0334497	[more]
CG11865	2.330e-22	31.75	gene:FBgn0028947 transcript:FBtr0080753	[more]
CG15254	1.044e-19	29.25	gene:FBgn0028949 transcript:FBtr0343718	[more]
CG15254	1.044e-19	29.25	gene:FBgn0028949 transcript:FBtr0080783	[more]

BLAST of Ast1 protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
hce2l2	1.113e-23	26.84	high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:...	[more]
he1.2	1.171e-23	32.00	hatching enzyme 1, tandem dupliate 2 [Source:ZFIN;...	[more]
hce2l2	2.767e-23	30.14	high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:...	[more]
he1.1	3.468e-23	31.56	hatching enzyme 1, tandem duplicate 1 [Source:ZFIN...	[more]
he1.1	3.468e-23	31.56	hatching enzyme 1, tandem duplicate 1 [Source:ZFIN...	[more]

BLAST of Ast1 protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
polr2l.2	2.329e-22	31.16	polymerase (RNA) II (DNA directed) polypeptide L, ...	[more]
ENSXETT00000022029.1	1.687e-21	29.73	astacin-like metalloendopeptidase [Source:NCBI gen...	[more]
kpna4	7.884e-21	29.74	karyopherin alpha 4 (importin alpha 3) [Source:Xen...	[more]
ENSXETT00000032191.1	4.023e-20	30.57	astacin-like metallo-endopeptidase 3 gene b1 [Sour...	[more]
ENSXETT00000032173.1	4.061e-20	30.57	astacin-like metallo-endopeptidase 3 gene b1 [Sour...	[more]

BLAST of Ast1 protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Astl	1.525e-16	26.61	astacin-like metalloendopeptidase (M12 family) [So...	[more]
Astl	2.848e-16	26.61	astacin-like metalloendopeptidase (M12 family) [So...	[more]
Astl	2.848e-16	26.61	astacin-like metalloendopeptidase (M12 family) [So...	[more]
Mep1b	1.502e-15	31.94	meprin 1 beta [Source:MGI Symbol;Acc:MGI:96964]	[more]
Tll1	1.786e-15	30.77	tolloid-like [Source:MGI Symbol;Acc:MGI:106923]	[more]

BLAST of Ast1 protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P55112\|NAS4_CAEEL	9.075e-23	31.28	Zinc metalloproteinase nas-4 OS=Caenorhabditis ele...	[more]
sp\|P31579\|LCE_ORYLA	2.155e-22	29.20	Low choriolytic enzyme OS=Oryzias latipes OX=8090 ...	[more]
sp\|P31581\|HCE2_ORYLA	1.057e-21	36.77	High choriolytic enzyme 2 OS=Oryzias latipes OX=80...	[more]
sp\|P31580\|HCE1_ORYLA	3.515e-21	42.24	High choriolytic enzyme 1 OS=Oryzias latipes OX=80...	[more]
sp\|P55113\|NAS7_CAEEL	6.486e-21	28.45	Zinc metalloproteinase nas-7 OS=Caenorhabditis ele...	[more]

BLAST of Ast1 protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A060Q785	0.000e+0	100.00	Metalloendopeptidase OS=Schmidtea mediterranea OX=...	[more]
A0A060Q714	5.773e-143	76.10	Metalloendopeptidase OS=Schmidtea mediterranea OX=...	[more]
A0A060Q786	9.484e-136	72.69	Metalloendopeptidase OS=Schmidtea mediterranea OX=...	[more]
I1ZIC3	2.467e-98	54.62	Metalloendopeptidase OS=Schmidtea mediterranea OX=...	[more]
A0A060Q750	1.356e-71	44.66	Metalloendopeptidase OS=Schmidtea mediterranea OX=...	[more]

BLAST of Ast1 protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
c6ast1	8.697e-26	30.67	low choriolytic enzyme-like [Source:NCBI gene;Acc:...	[more]
hce2l1	8.882e-26	33.51	high choriolytic enzyme 1-like [Source:NCBI gene;A...	[more]
ENSAMXT00000033223.1	1.037e-25	30.05	high choriolytic enzyme 1-like [Source:NCBI gene;A...	[more]
ENSAMXT00000056363.1	1.037e-25	30.05	high choriolytic enzyme 1-like [Source:NCBI gene;A...	[more]
ENSAMXT00000044782.1	1.037e-25	30.05	high choriolytic enzyme 1-like [Source:NCBI gene;A...	[more]

BLAST of Ast1 protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2


Match Name	E-value	Identity	Description
ENSPMAT00000007253.1	1.301e-16	30.77	pep scaffold:Pmarinus_7.0:GL476910:54360:144194:-1...	[more]
ENSPMAT00000004913.1	1.585e-9	37.84	pep scaffold:Pmarinus_7.0:GL478359:22377:26907:1 g...	[more]

BLAST of Ast1 protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Ast1 protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO42421	4.457e-23	31.09	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO34721	2.106e-22	29.95	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO37770	3.662e-22	28.63	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO29086	5.504e-21	35.88	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO41420	6.030e-20	28.13	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Ast1 protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
mc6ast5	2.965e-27	37.42	astacin like metallo-protease [Source:NCBI gene;Ac...	[more]
mc6ast4	5.708e-26	29.09	astacin like metallo-protease [Source:NCBI gene;Ac...	[more]
lce	1.838e-23	29.20	protease [Source:NCBI gene;Acc:100049451]	[more]
lce	2.404e-23	29.20	protease [Source:NCBI gene;Acc:100049451]	[more]
mc6ast3	3.390e-23	27.56	astacin like metallo-protease [Source:NCBI gene;Ac...	[more]

BLAST of Ast1 protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000015174.1	0.000e+0	100.00	SMESG000015174.1	[more]
SMESG000015174.1	0.000e+0	100.00	SMESG000015174.1	[more]
SMESG000015174.1	0.000e+0	100.00	SMESG000015174.1	[more]
SMESG000072791.1	2.637e-139	72.69	SMESG000072791.1	[more]
SMESG000072807.1	2.967e-136	73.88	SMESG000072807.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Cross References

External references for this transcript


Database	Accession
SmedGD GBrowse	SMED30002195
GenBank	CCW36839.1

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30002195 ID=SMED30002195|Name=Ast1 protein|organism=Schmidtea mediterranea sexual|type=transcript|length=787bp
CATGCAAAAAATGTTTTTGATGTGTTTGGCGATTGCCGTTATCGGTTACG
TTTCTGGTCAATGTGGGTGCCCTAGACAGAAACTGCTGAAGAGGTCTGTA
GATAGCATGAAAATGGGAGACATGTTATTGGATAAATCTGATATTGCACT
AATAAACGGCTTTAAAAACCATTATGCTAATACTAAAAAATGGCCTAACA
ACAATGTTGTATACTCTTTTGCTGGAAACTTTCCACGAGACAAGATTCCA
GTCGTTAGACAATGCTTGGGAGAATTGCAACGAGATTTGGGAAACTGTGT
CAAGTTCACCGAATCCAATCAAGGCCATTATATTGAAGTCAACTCAAAAC
AAGAAGGCTGCTATTCACTGTTGGGATATGCCGCAGGAATGCCTACCCAA
CCATTGAATTTGGAAAATCCCGGATGCATGTATTCAAAAGGTACAGTTAA
GCACGAATTCATTCATGCACTCGGATTCATGCATACACATATGAGAAAAG
ACAGAGACAATCATATTACTATTAATTGGGCAAGAATTGAAACAAGTCAA
TGCAGTCAGTTTACGAAATGCGACGGATGTGAACTTGATGGACCATACGA
AACCACCAGTGTAATGCACTATAACAGTGAAGGATTTGCTTGTGATCCTC
GAGAAAAAGTTGTTTTAAGAAAAGATGGAGGAGCGATCGGTTATAATCAC
GACACAACTCCTAATGATTTGAACATGATAAGAAACTTTTATGGATGTTA
AAGACGTTGAAAAACAAATATATGTAAAATTTGCTTG

protein sequence of SMED30002195-orf-1

>SMED30002195-orf-1 ID=SMED30002195-orf-1|Name=SMED30002195-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=250bp
MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIAL
INGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCV
KFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVK
HEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYE
TTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC*

Aliases

The feature 'Ast1 protein' has the following synonyms


Synonym
Ast1

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000418	head
PLANA:0000420	parapharyngeal region

Vocabulary: **INTERPRO**
Term	Definition
IPR001506	Peptidase_M12A
IPR006026	Peptidase_Metallo
IPR024079	MetalloPept_cat_dom_sf

Vocabulary: **molecular function**
Term	Definition
GO:0004222	metalloendopeptidase activity
GO:0008270	zinc ion binding
GO:0008237	metallopeptidase activity
GO:0008233	peptidase activity
GO:0016787	hydrolase activity
GO:0046872	metal ion binding

Vocabulary: **biological process**
Term	Definition
GO:0006508	proteolysis

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001506	Peptidase M12A	PRINTS	PR00480	ASTACIN	coord: 162..179 score: 42.22 coord: 143..161 score: 45.53 coord: 235..248 score: 21.79 coord: 198..213 score: 43.33
IPR001506	Peptidase M12A	PFAM	PF01400	Astacin	coord: 62..249 e-value: 2.8E-39 score: 134.7
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 60..203 e-value: 1.4E-21 score: 87.7
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	G3DSA:3.40.390.10		coord: 25..249 e-value: 1.8E-47 score: 163.8
None	No IPR available	PANTHER	PTHR10127	DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING	coord: 40..249
None	No IPR available	PROSITE	PS51864	ASTACIN	coord: 49..249 score: 41.17
None	No IPR available	SIGNALP_EUK	SignalP-noTM	SignalP-noTM	coord: 1..19 score: 0.859
None	No IPR available	SUPERFAMILY	SSF55486	Metalloproteases ("zincins"), catalytic domain	coord: 60..249

Main menu

Ast1 protein

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Owlery

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