Spondin-1
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30002168 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 12
Homology
BLAST of Spondin-1 vs. Ensembl Human
Match: SPON1 (spondin 1 [Source:HGNC Symbol;Acc:HGNC:11252]) HSP 1 Score: 154.836 bits (390), Expect = 5.094e-38 Identity = 146/515 (28.35%), Postives = 224/515 (43.50%), Query Frame = 2
Query: 149 AFSTRFNLYTNHEKEKLTKITSYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRER 1663
A TR YT Y P +Y +T+S + T+ L N + EE +AG F + +T++ CP A + + + +WIAP C+ + + +I + +CEQ ST + K P+ C TA YR+T W+ H KD+P + + I +G SHS + ++ GG A+EGV QV GS +++++ + + ++TVI A++ A N R SA V+ T+HL+SFL + PSPDW GLS DLC C W +K + L PWDAG SG TY + N+ T Q I P+TS +PF G I + V + I + N ++++ I+ D A + K D C+ S WS WS+CS S K +++LK + + + T + + C + TC+ W WSPC+ +C G+ RER
Sbjct: 50 AQGTRREGYTEFSLRVEGDPDFYKPGTSYRVTLSAAPPSYFRGFTL---IALRENREGDKEE------DHAG--TFQIIDEEETQFMSNCPV-AVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQD-------------STFDGVTDK--PILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAI---IGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEE-------KDEDDTPETCIYSNWSPWSACSSSTCDKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSISCGM-GMRSRER 526
BLAST of Spondin-1 vs. Ensembl Human
Match: SPON2 (spondin 2 [Source:HGNC Symbol;Acc:HGNC:11253]) HSP 1 Score: 93.2041 bits (230), Expect = 7.699e-20 Identity = 62/188 (32.98%), Postives = 95/188 (50.53%), Query Frame = 2
Query: 668 CNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKI--TQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDA-SSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWP 1219
C+ + A Y +T KW+ K +P + P + LGA+HS++++++ + G+ G L K+ AG + +V S A S T + SA + V LVSF+ R+ PSPDW G+ DLC+G +W+E+ + LYP+DAG SG T+ + N T Q + ITSS P
Sbjct: 35 CSARALAKYSITFTGKWSQTAFPKQYPL---FRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGEAWALMKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGD-RWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSP 218
BLAST of Spondin-1 vs. Ensembl Human
Match: SPON2 (spondin 2 [Source:HGNC Symbol;Acc:HGNC:11253]) HSP 1 Score: 93.2041 bits (230), Expect = 7.699e-20 Identity = 62/188 (32.98%), Postives = 95/188 (50.53%), Query Frame = 2
Query: 668 CNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKI--TQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDA-SSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWP 1219
C+ + A Y +T KW+ K +P + P + LGA+HS++++++ + G+ G L K+ AG + +V S A S T + SA + V LVSF+ R+ PSPDW G+ DLC+G +W+E+ + LYP+DAG SG T+ + N T Q + ITSS P
Sbjct: 35 CSARALAKYSITFTGKWSQTAFPKQYPL---FRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGEAWALMKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGD-RWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSP 218
BLAST of Spondin-1 vs. Ensembl Human
Match: SPON2 (spondin 2 [Source:HGNC Symbol;Acc:HGNC:11253]) HSP 1 Score: 93.2041 bits (230), Expect = 7.699e-20 Identity = 62/188 (32.98%), Postives = 95/188 (50.53%), Query Frame = 2
Query: 668 CNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKI--TQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDA-SSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWP 1219
C+ + A Y +T KW+ K +P + P + LGA+HS++++++ + G+ G L K+ AG + +V S A S T + SA + V LVSF+ R+ PSPDW G+ DLC+G +W+E+ + LYP+DAG SG T+ + N T Q + ITSS P
Sbjct: 35 CSARALAKYSITFTGKWSQTAFPKQYPL---FRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGEAWALMKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGD-RWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSP 218
BLAST of Spondin-1 vs. Ensembl Human
Match: SPINT1 (serine peptidase inhibitor, Kunitz type 1 [Source:HGNC Symbol;Acc:HGNC:11246]) HSP 1 Score: 74.3294 bits (181), Expect = 4.515e-14 Identity = 45/123 (36.59%), Postives = 63/123 (51.22%), Query Frame = 2
Query: 1823 RFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPET----------YPNRCG--VSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
R+YYD + C +F+Y GC G NN+ EECIL CR + +RR P+T +P+ G V + C + S RWYY+ ++ C RF +GGC GN NNF + +C C
Sbjct: 83 RWYYDPTEQICKSFVYGGCLGNKNNYLREEECILACRGVQGPSMERRH--PDTSGFDELQRIHFPSDKGHCVDLPDTGLC--KESIPRWYYNPFSEHCARFTYGGCYGNKNNFEEEQQCLESC 201
BLAST of Spondin-1 vs. Ensembl Celegans
Match: spon-1 (SPONdin (Extracellular matrix glycoprotein) family [Source:UniProtKB/TrEMBL;Acc:Q19305]) HSP 1 Score: 147.902 bits (372), Expect = 2.708e-36 Identity = 163/665 (24.51%), Postives = 275/665 (41.35%), Query Frame = 2
Query: 212 SYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEYCPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDASSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTR-QTPICPITSSWPKDNPFTVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLTNTES--CSSK---CSTVISFET-CSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASERCK--PDVICKEYVYPGDS-CSWSSP------SQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCL 2158
++P + Y +++ + + T+K V + E + S W +VV A + C Q V W AP++ + C+ + I ++ E + +C QK ++ +K DP C A Y + W+ H KD+P + + T LG+SHS+ ++++ +GG++ +G+ ++ G+ + + + + + +++ + L + VVN H VS PSPDWC GLS +LC C W E+ L P+DAG SG TY++ NE T + PI IT+ +PF N K I + +++ N ++ C S + IL+ EA+ N +S + D+ R C++++W WS CS +CGKG + R R + Q TE C++K C +F + C WG W C+ C G+ R R F+N K + + + C PD +CK + S CS S ++ F+Y C N + C++ + E + + +C G F R++Y++ +C RF F GC+GN N F T+ EC+ +CL
Sbjct: 62 GFVPGEIYKVSI----RGWRTQYTVKTFRGFVVSSLFE-DNTSAGSW----QVVKGHGDARISPGCRQSGVSHANLKSKTSVHMMWKAPEVSSGCVVFRASVIETKYIWFTEAEGLTVKLCIQKGTQI--------------LKPVDDPSATCCACDIAQYDLEFTGIWSKNTHPKDYPTLEHLT-HFTDMLGSSHSSNYSLWTIGGISTDGMKEIAEWGNTYKAEAEAKAKASEVRSLMKVKGLWFPDVQGTTKSQFVVNKYHHFVSLATMFGPSPDWCVGLSSVNLCLPDCTWAEERTFELQPFDAGTDSGPTYMSPNEPTEPREPIHWITTKLNPLSPF--YNSKSDTIPTLAKVILRRKNVTSSE-CKS-DDILKAEAH-NITNTSEDEEYKDR-RECMMTQWEPWSLCSATCGKGIRIRSRVYVFPIKAQVFHCHRQTTEKQFCNAKINECENSEAFSSKCQVSSWGSWGECSVQCGH-GMRSRNRTFLNPATKSGDCSVDLERKDICVGENGDDCNVTPDPLCKTTAWSDWSPCSASCDEGVRVRTRLFFYSEHEKRCMHV----------NLQEKDTCVMQSCRRFIEINSEEICQEDKQAGQCA-----GNF-------PRYWYNHEKTQCERFIFTGCKGNRNQFETEEECKQICL 673 HSP 2 Score: 62.7734 bits (151), Expect = 1.125e-9 Identity = 39/121 (32.23%), Postives = 59/121 (48.76%), Query Frame = 2
Query: 1586 CSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASER----CKPDVICKEYVYPGDSCSWSSPSQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRP 1936
C W WSPC+A+C + G+ R R+F + + CM + ++ C + S R + IC+E G C+ + P R++Y+ C FI+ GC G N F T EEC +C P
Sbjct: 559 CKTTAWSDWSPCSASCDE-GVRVRTRLFFYSEHEKRCMHVN-LQEKDTCVMQSCRRFIEINSEEICQEDKQAGQ-CAGNFP--RYWYNHEKTQCERFIFTGCKGNRNQFETEEECKQICLP 674
BLAST of Spondin-1 vs. Ensembl Celegans
Match: E01G6.1 (pep chromosome:WBcel235:X:12260881:12266537:1 gene:WBGene00008449.1 transcript:E01G6.1.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:E01G6.1) HSP 1 Score: 68.1662 bits (165), Expect = 2.554e-11 Identity = 46/165 (27.88%), Postives = 67/165 (40.61%), Query Frame = 2
Query: 1757 PDVICKEYVYPGDSCSWSSPS-QRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCR-----------------------------PKLKETDQRRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCLR 2161
P++ C E V G C + QRFY++ S+ C F Y GC G NNF T ++C C P E + + P + N C +M+ G C + + W+YD K+C +F + GC G N F TK C C++
Sbjct: 434 PELTCNEMVSAGTPCFGRGLTIQRFYFNPSTQKCQPFHYYGCSGNGNNFETVDQCQNFCLHSADSACDGLVPLKNPNSELQRCSEEDPCPAGYECNDSSYCCPSSE-NACNANMSRGNGCKGSTQRSMWFYDKSKKKCSQFVYNGCGGTPNRFTTKVACTESCVQ 597
BLAST of Spondin-1 vs. Ensembl Celegans
Match: Y55F3BR.2 (pep chromosome:WBcel235:IV:862099:874724:1 gene:WBGene00021939.1 transcript:Y55F3BR.2b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y55F3BR.2) HSP 1 Score: 68.1662 bits (165), Expect = 2.788e-11 Identity = 58/225 (25.78%), Postives = 93/225 (41.33%), Query Frame = 2
Query: 1592 FRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASERC--KPDVICKEYVYPGDSCSWSSPSQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCR-----PKLKET------------------------------DQRRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
F + + C C++ H + C + ++ ++ CA+ + C P +C E + GD C S ++F+Y+ + TC +F+Y GC G N F++ EC C+ PK + D + + C + + GI C S SS R+YY+N KEC + F GC GN+NNF + +CQN C
Sbjct: 190 FNNFQNQADCQLFCARLQCPHGSPLTNGNGSPQRCARDTDCPSTHTCAMEHQVCCPTPQTLCTEPLRVGD-CKQSV--RQFWYNAETKTCESFLYTGCQGNNNRFNSLNECQSYCKNINAEPKCPQGRAYVDFSGKFMQCGEGLGGTACPANYECTFDGLVYGCCPSKAYTCSLQVNKGIGCGS-GSSYRYYYNNQAKECQSYLFLGCDGNSNNFPSIEKCQNYC 410 HSP 2 Score: 53.9138 bits (128), Expect = 5.903e-7 Identity = 37/137 (27.01%), Postives = 56/137 (40.88%), Query Frame = 2
Query: 1817 SQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPETYPNRCG----------VSMTWGIFC--------------DSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
S R+YY+ + G C+ F + G G NNF +C L C +L+ P RC +M + C D + S +++Y+ K C F + GC+GN N F + ECQ+ C
Sbjct: 166 STRYYYNPTDGQCHPFTFNGFLGNFNNFQNQADCQLFCA-RLQCPHGSPLTNGNGSPQRCARDTDCPSTHTCAMEHQVCCPTPQTLCTEPLRVGDCKQSVRQFWYNAETKTCESFLYTGCQGNNNRFNSLNECQSYC 301
BLAST of Spondin-1 vs. Ensembl Celegans
Match: Y55F3BR.2 (pep chromosome:WBcel235:IV:857327:874724:1 gene:WBGene00021939.1 transcript:Y55F3BR.2a.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y55F3BR.2) HSP 1 Score: 68.1662 bits (165), Expect = 2.961e-11 Identity = 58/225 (25.78%), Postives = 93/225 (41.33%), Query Frame = 2
Query: 1592 FRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASERC--KPDVICKEYVYPGDSCSWSSPSQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCR-----PKLKET------------------------------DQRRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
F + + C C++ H + C + ++ ++ CA+ + C P +C E + GD C S ++F+Y+ + TC +F+Y GC G N F++ EC C+ PK + D + + C + + GI C S SS R+YY+N KEC + F GC GN+NNF + +CQN C
Sbjct: 483 FNNFQNQADCQLFCARLQCPHGSPLTNGNGSPQRCARDTDCPSTHTCAMEHQVCCPTPQTLCTEPLRVGD-CKQSV--RQFWYNAETKTCESFLYTGCQGNNNRFNSLNECQSYCKNINAEPKCPQGRAYVDFSGKFMQCGEGLGGTACPANYECTFDGLVYGCCPSKAYTCSLQVNKGIGCGS-GSSYRYYYNNQAKECQSYLFLGCDGNSNNFPSIEKCQNYC 703 HSP 2 Score: 54.299 bits (129), Expect = 4.752e-7 Identity = 37/137 (27.01%), Postives = 56/137 (40.88%), Query Frame = 2
Query: 1817 SQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPETYPNRCG----------VSMTWGIFC--------------DSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
S R+YY+ + G C+ F + G G NNF +C L C +L+ P RC +M + C D + S +++Y+ K C F + GC+GN N F + ECQ+ C
Sbjct: 459 STRYYYNPTDGQCHPFTFNGFLGNFNNFQNQADCQLFCA-RLQCPHGSPLTNGNGSPQRCARDTDCPSTHTCAMEHQVCCPTPQTLCTEPLRVGDCKQSVRQFWYNAETKTCESFLYTGCQGNNNRFNSLNECQSYC 594
BLAST of Spondin-1 vs. Ensembl Celegans
Match: Y55F3BR.2 (pep chromosome:WBcel235:IV:858930:874724:1 gene:WBGene00021939.1 transcript:Y55F3BR.2a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y55F3BR.2) HSP 1 Score: 68.1662 bits (165), Expect = 2.961e-11 Identity = 58/225 (25.78%), Postives = 93/225 (41.33%), Query Frame = 2
Query: 1592 FRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASERC--KPDVICKEYVYPGDSCSWSSPSQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCR-----PKLKET------------------------------DQRRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
F + + C C++ H + C + ++ ++ CA+ + C P +C E + GD C S ++F+Y+ + TC +F+Y GC G N F++ EC C+ PK + D + + C + + GI C S SS R+YY+N KEC + F GC GN+NNF + +CQN C
Sbjct: 483 FNNFQNQADCQLFCARLQCPHGSPLTNGNGSPQRCARDTDCPSTHTCAMEHQVCCPTPQTLCTEPLRVGD-CKQSV--RQFWYNAETKTCESFLYTGCQGNNNRFNSLNECQSYCKNINAEPKCPQGRAYVDFSGKFMQCGEGLGGTACPANYECTFDGLVYGCCPSKAYTCSLQVNKGIGCGS-GSSYRYYYNNQAKECQSYLFLGCDGNSNNFPSIEKCQNYC 703 HSP 2 Score: 54.299 bits (129), Expect = 4.752e-7 Identity = 37/137 (27.01%), Postives = 56/137 (40.88%), Query Frame = 2
Query: 1817 SQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPETYPNRCG----------VSMTWGIFC--------------DSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
S R+YY+ + G C+ F + G G NNF +C L C +L+ P RC +M + C D + S +++Y+ K C F + GC+GN N F + ECQ+ C
Sbjct: 459 STRYYYNPTDGQCHPFTFNGFLGNFNNFQNQADCQLFCA-RLQCPHGSPLTNGNGSPQRCARDTDCPSTHTCAMEHQVCCPTPQTLCTEPLRVGDCKQSVRQFWYNAETKTCESFLYTGCQGNNNRFNSLNECQSYC 594
BLAST of Spondin-1 vs. Ensembl Fly
Match: fat-spondin (gene:FBgn0026721 transcript:FBtr0086975) HSP 1 Score: 133.265 bits (334), Expect = 1.514e-31 Identity = 160/700 (22.86%), Postives = 270/700 (38.57%), Query Frame = 2
Query: 212 SYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEYCPQRA-YVQYLYTQPQ-HVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQY---LGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDASSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQTP--ICPITSSWPKDNPFTVMNGKIKNIGVVTFQLIQE---YNKN-DNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKG--KSEQCIES--------------------SLTNTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASERC-KPDVICKEYVYPGDSCSWSSPSQRFYYDFS--SGTC--------------NAFIYKGCYGGLNNFHTYEECI--LLCRPKLKETDQRRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
Y+P KTY++ L H K+ + + + HT + G F + S T++ + V P+ V W+AP+ + C+ ++ ++ + S ICE+K D A+ K+C A Y E W+ H KD+P ++ +T + +GASH + F+ + +A G + GS L+ + G + T+I A L + S+ V+ VS ++ PSPDW G+S DLC C W+E L+PWDAG SG +Y++ N +T Q P + IT+ +P+D N K + + + ++ ++N D+++ ++ + D+A + + ++ + C +S S K + E C+ + +L N++S S C W WS C+A+C GI R R F+N + C + ++ + +C +PD ++ P C S + D+S S TC N + C + H +EC+ + C ++ P+ P R G M R+ YD N+ CY F +GGCRGN NNF T+ +C N C
Sbjct: 54 GYVPGKTYNLL--LLGSRTHLKVQHFTHFTITAEAHTGARRPQAASPRRVGR--FQLFSDSLTQFNDRCVNTVSEADDLPKTEVQVMWVAPESGSGCVSLSAMVYEGPRAWFADDGNLSTVICERK--------PDAAAAQ-----------KECCACDEAKYSFVFEGIWSNETHPKDYP----FAIWLTHFSDVIGASHESNFSFWGENHIATAGFRSLAEWGSPAALETELRANGPKLRTLIKAAGLWYPNVNQNTSSKFRVDRKHPKVSLVSMFGPSPDWVVGISGLDLCTEDCSWKESMDFDLFPWDAGTDSGISYMSPNSET-QPPERMYRITTMYPEDPRAPFYNPKSREMTPLAKLYLRREKIVSRNCDDEFLQALQLEVSDDAEEQDTRAECRVGDYSAWSPCSVSCGKGIRMRSRQYLYPAAADQNKCARQLVAKEMCVAAIPECADGPAQSKDRDDDEGENLANSQSLVG--SNGEGAGLCKTSPWSVWSECSASCG-IGITMRTRTFVNHLGRKRCPHITIVEKN--------KCMRPDCTYEQVELPDPQCPTSQ-----WSDWSPCSSTCGRGVTIRTRLLLLENGPDKESCTQRM-ELHQQKECVNPIDCHINAEQAKDICVQAPDPGPCR-GTYM-------------RYAYDPQNQHCYSFTYGGCRGNRNNFLTENDCLNTC 694
BLAST of Spondin-1 vs. Ensembl Fly
Match: CG17739 (gene:FBgn0033710 transcript:FBtr0087893) HSP 1 Score: 132.109 bits (331), Expect = 4.411e-31 Identity = 84/294 (28.57%), Postives = 146/294 (49.66%), Query Frame = 2
Query: 452 HVTFYWIAPKIPN---FCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDASSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQTP---ICPITSSWPKD--NPFTVMNG-KIKNIGVVTFQLIQEYNKN 1306
V +W+AP P +R TV+ + + F + +CE++ + +D + DP C+ A Y +T E KW+ H KD+P + + + + +GASH+ ++ + G +A+EG+ +V +GS L+ + I T+I AR + + T K A+ V++ HL+S ++ + PSPDW G+S +LC +C W E + +LYPWDAG SG +Y++ ++ Q P + I S++P D +PF G ++K + + + Y KN
Sbjct: 122 RVDVFWVAPSSPGQGCILLRATVMQHRDVWFMDDGFLTKR--MCEEEVDDIDTQPS------------IVDPCCACD---EAKYELTFEGKWSRHTHPKDFPA-NSWRTRFSDIIGASHTLDYRFWQYGELASEGLREVAEHGSTRTLESELKDQSEHIRTIIKARGIAYPNVTGKTFAVFRVDSNHHLISLVSMVDPSPDWIVGVSGLELCLPNCSWVENKVHNLYPWDAGTDSGPSYMSADQP--QVPPDVVRRIKSNFPNDPRSPFYDPTGAQMKPLATLHINRRRLYEKN 395
BLAST of Spondin-1 vs. Ensembl Fly
Match: CG30203 (gene:FBgn0050203 transcript:FBtr0273303) HSP 1 Score: 121.709 bits (304), Expect = 8.914e-28 Identity = 78/273 (28.57%), Postives = 145/273 (53.11%), Query Frame = 2
Query: 452 HVTFYWIAPKIPN-FCIRITV-ILKMNDL--ISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYK-AGNG-IMTVILARSLDASSKTNKRS-ALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQTP---ICPITSSWPKD--NPF 1234
++ WIAP+ P+ C+ I +++ D+ + + +R IC ++ ++++ + T P++ C A Y + +E KW HWKD+P +D + ++ + +GASHS+ + + GG A++G+ ++ +G+ L+ + + NG + T+I A + KT + A ++ H +S A++ PSPDW G++ +LC +C W E+ +++LYPWD G SG +Y++ +E Q P + ITSS+P D +PF
Sbjct: 119 YIAVSWIAPRNPDSGCVLIKAGVVQHRDVWFLDDGFLTKR---ICPEEIDELNSL---------------TPPLETCCACDEAKYEIVLERKWARNTHWKDFPSED-WRTRLGEVIGASHSHSYRYWAYGGRASKGMKELAEHGATRTLENEIRENTQNGEVRTIIKAPGIPYRPKTFGTTLANARLDPVHHQISLAAKIDPSPDWILGVAGLELCLSNCTWLERKVLNLYPWDIGTDSGPSYMSSDEP--QVPPDVVRRITSSYPSDHRSPF 370
BLAST of Spondin-1 vs. Ensembl Fly
Match: CG30046 (gene:FBgn0050046 transcript:FBtr0087895) HSP 1 Score: 114.005 bits (284), Expect = 1.608e-25 Identity = 79/293 (26.96%), Postives = 143/293 (48.81%), Query Frame = 2
Query: 452 HVTFYWIAPKIP-NFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYK--AGNGIMTVILARSLDASSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQTP---ICPITSSWPKD--NPFTVMNG-KIKNIGVVTFQLIQEYNK 1303
H+ W+AP P + C+ I ++ + + + ICE+ D S A P C+ A Y + E W+ H KD+P + G+ + + LGA+HS+++ + G +A+ + + + S L+++F I T+I AR + ++K A + V+ H+VSF +++ PSPDW G++ +LC +C W E+ +++LYPWD G SG TY + ++ Q P I + S +P D +PF ++G +K + ++T + + Y +
Sbjct: 126 HMHLTWVAPSEPGSGCVLIRATVQQHREVWHMDDGGLTKRICEEV--------TDDVESQPTAPAAVDVPCCACD---EARYELIFEGVWSRNLHPKDFPTR-GWETRFCELLGAAHSSDYRFWESGELASVAMKEYAEHCSSRLLEREFSINFRDQKIRTIIKARGPSYPNISSKTMASVRVDPIHHMVSFASKIEPSPDWIVGVTGLELCLRNCTWMEEKVINLYPWDVGTDSGPTYTSSDQP--QVPPDVIRRMRSDFPNDPRSPFYDISGTPMKPMAILTVKRQRIYER 404
BLAST of Spondin-1 vs. Ensembl Fly
Match: CG30046 (gene:FBgn0050046 transcript:FBtr0339627) HSP 1 Score: 114.005 bits (284), Expect = 1.608e-25 Identity = 79/293 (26.96%), Postives = 143/293 (48.81%), Query Frame = 2
Query: 452 HVTFYWIAPKIP-NFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYK--AGNGIMTVILARSLDASSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQTP---ICPITSSWPKD--NPFTVMNG-KIKNIGVVTFQLIQEYNK 1303
H+ W+AP P + C+ I ++ + + + ICE+ D S A P C+ A Y + E W+ H KD+P + G+ + + LGA+HS+++ + G +A+ + + + S L+++F I T+I AR + ++K A + V+ H+VSF +++ PSPDW G++ +LC +C W E+ +++LYPWD G SG TY + ++ Q P I + S +P D +PF ++G +K + ++T + + Y +
Sbjct: 126 HMHLTWVAPSEPGSGCVLIRATVQQHREVWHMDDGGLTKRICEEV--------TDDVESQPTAPAAVDVPCCACD---EARYELIFEGVWSRNLHPKDFPTR-GWETRFCELLGAAHSSDYRFWESGELASVAMKEYAEHCSSRLLEREFSINFRDQKIRTIIKARGPSYPNISSKTMASVRVDPIHHMVSFASKIEPSPDWIVGVTGLELCLRNCTWMEEKVINLYPWDVGTDSGPTYTSSDQP--QVPPDVIRRMRSDFPNDPRSPFYDISGTPMKPMAILTVKRQRIYER 404
BLAST of Spondin-1 vs. Ensembl Zebrafish
Match: spon1b (spondin 1b [Source:ZFIN;Acc:ZDB-GENE-000427-10]) HSP 1 Score: 152.91 bits (385), Expect = 1.191e-37 Identity = 143/534 (26.78%), Postives = 227/534 (42.51%), Query Frame = 2
Query: 113 GFCDIQFPKSYVA---FSTRFNLYTNHEKEKLTKITSYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLI---SESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYR-KLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRER 1663
GFC I ++ A F L E E +Y P TY +++ + + T+ + E +S D+ F + S T++ CP A + + + + +W AP + C+ + + +I E +R +CE+ D A T P ++C TA YR+T W+ H KD+P + + + +GASH+ + ++ GG A+EGV QV GS +++++ + G+ +MTVI ++ A N R SA V+ +HL+SFL L PSPDW GLS DLC C W +K + L PWDAG SG +Y + N+ + Q I P+TS +PF G + +G V + I + N ++I+ I+ D A + K D T + S S + R R ++LK + + + T + E C + TC W WSPC+A+C G+ RER
Sbjct: 38 GFCRILRTQARSARRDGQNEFRLKVEGEPE------TYQPGSTYRVSLYASSPSYFRGFTLIAL-------KEGREGVSPDDYAG----TFKLIDESDTQFMTNCPP-AVTESIPRRRTRIQVFWTAPPSGSGCVTLKASVVQRRIIFFQDEGSLTKR---LCEK----------DPVAITE-------KPAQECCACGTAKYRVTFYGNWSEKLHPKDYPRRANH---WSALIGASHTRSYVLWEYGGFASEGVKQVSEYGSPVKMEEEIRQKGDDVMTVIKTKAQWPAWQPLNIRAAPSAEFSVDRVRHLMSFLTMLGPSPDWNVGLSGEDLCTRDCGWVQKLVKDLVPWDAGTDSGVSYESPNKPSIPQERIRPLTSLDHPQSPFYDPSGGPMTPLGRVVVERIARKGEQCNTVPDTIDDIVADIAQEE-------KEEGDNTPETCIYSNWSPWSACSSSTCDKGRRMRQRMLKAQLDPNLPCLHTQDFEPCMGPGCSEEEASTCMMSEWISWSPCSASCGM-GMRSRER 522
BLAST of Spondin-1 vs. Ensembl Zebrafish
Match: spon1b (spondin 1b [Source:ZFIN;Acc:ZDB-GENE-000427-10]) HSP 1 Score: 152.91 bits (385), Expect = 1.273e-37 Identity = 143/534 (26.78%), Postives = 227/534 (42.51%), Query Frame = 2
Query: 113 GFCDIQFPKSYVA---FSTRFNLYTNHEKEKLTKITSYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLI---SESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYR-KLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRER 1663
GFC I ++ A F L E E +Y P TY +++ + + T+ + E +S D+ F + S T++ CP A + + + + +W AP + C+ + + +I E +R +CE+ D A T P ++C TA YR+T W+ H KD+P + + + +GASH+ + ++ GG A+EGV QV GS +++++ + G+ +MTVI ++ A N R SA V+ +HL+SFL L PSPDW GLS DLC C W +K + L PWDAG SG +Y + N+ + Q I P+TS +PF G + +G V + I + N ++I+ I+ D A + K D T + S S + R R ++LK + + + T + E C + TC W WSPC+A+C G+ RER
Sbjct: 38 GFCRILRTQARSARRDGQNEFRLKVEGEPE------TYQPGSTYRVSLYASSPSYFRGFTLIAL-------KEGREGVSPDDYAG----TFKLIDESDTQFMTNCPP-AVTESIPRRRTRIQVFWTAPPSGSGCVTLKASVVQRRIIFFQDEGSLTKR---LCEK----------DPVAITE-------KPAQECCACGTAKYRVTFYGNWSEKLHPKDYPRRANH---WSALIGASHTRSYVLWEYGGFASEGVKQVSEYGSPVKMEEEIRQKGDDVMTVIKTKAQWPAWQPLNIRAAPSAEFSVDRVRHLMSFLTMLGPSPDWNVGLSGEDLCTRDCGWVQKLVKDLVPWDAGTDSGVSYESPNKPSIPQERIRPLTSLDHPQSPFYDPSGGPMTPLGRVVVERIARKGEQCNTVPDTIDDIVADIAQEE-------KEEGDNTPETCIYSNWSPWSACSSSTCDKGRRMRQRMLKAQLDPNLPCLHTQDFEPCMGPGCSEEEASTCMMSEWISWSPCSASCGM-GMRSRER 522
BLAST of Spondin-1 vs. Ensembl Zebrafish
Match: spon1b (spondin 1b [Source:ZFIN;Acc:ZDB-GENE-000427-10]) HSP 1 Score: 152.525 bits (384), Expect = 1.491e-37 Identity = 143/534 (26.78%), Postives = 227/534 (42.51%), Query Frame = 2
Query: 113 GFCDIQFPKSYVA---FSTRFNLYTNHEKEKLTKITSYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLI---SESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYR-KLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRER 1663
GFC I ++ A F L E E +Y P TY +++ + + T+ + E +S D+ F + S T++ CP A + + + + +W AP + C+ + + +I E +R +CE+ D A T P ++C TA YR+T W+ H KD+P + + + +GASH+ + ++ GG A+EGV QV GS +++++ + G+ +MTVI ++ A N R SA V+ +HL+SFL L PSPDW GLS DLC C W +K + L PWDAG SG +Y + N+ + Q I P+TS +PF G + +G V + I + N ++I+ I+ D A + K D T + S S + R R ++LK + + + T + E C + TC W WSPC+A+C G+ RER
Sbjct: 38 GFCRILRTQARSARRDGQNEFRLKVEGEPE------TYQPGSTYRVSLYASSPSYFRGFTLIAL-------KEGREGVSPDDYAG----TFKLIDESDTQFMTNCPP-AVTESIPRRRTRIQVFWTAPPSGSGCVTLKASVVQRRIIFFQDEGSLTKR---LCEK----------DPVAITE-------KPAQECCACGTAKYRVTFYGNWSEKLHPKDYPRRANH---WSALIGASHTRSYVLWEYGGFASEGVKQVAEYGSPVKMEEEIRQKGDDVMTVIKTKAQWPAWQPLNIRAAPSAEFSVDRVRHLMSFLTMLGPSPDWNVGLSGEDLCTRDCGWVQKLVKDLVPWDAGTDSGVSYESPNKPSIPQERIRPLTSLDHPQSPFYDPSGGPMTPLGRVVVERIARKGEQCNTVPDTIDDIVADIAQEE-------KEEGDNTPETCIYSNWSPWSACSSSTCDKGRRMRQRMLKAQLDPNLPCLHTQDFEPCMGPGCSEEEASTCMMSEWISWSPCSASCGM-GMRSRER 522
BLAST of Spondin-1 vs. Ensembl Zebrafish
Match: spon1b (spondin 1b [Source:ZFIN;Acc:ZDB-GENE-000427-10]) HSP 1 Score: 149.828 bits (377), Expect = 1.005e-36 Identity = 126/445 (28.31%), Postives = 198/445 (44.49%), Query Frame = 2
Query: 371 VFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLI---SESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYR-KLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRER 1663
F + S T++ CP A + + + + +W AP + C+ + + +I E +R +CE+ D A T P ++C TA YR+T W+ H KD+P + + + +GASH+ + ++ GG A+EGV QV GS +++++ + G+ +MTVI ++ A N R SA V+ +HL+SFL L PSPDW GLS DLC C W +K + L PWDAG SG +Y + N+ + Q I P+TS +PF G + +G V + I + N ++I+ I+ D A + K D T + S S + R R ++LK + + + T + E C + TC W WSPC+A+C G+ RER
Sbjct: 73 TFKLIDESDTQFMTNCPP-AVTESIPRRRTRIQVFWTAPPSGSGCVTLKASVVQRRIIFFQDEGSLTKR---LCEK----------DPVAITE-------KPAQECCACGTAKYRVTFYGNWSEKLHPKDYPRRANH---WSALIGASHTRSYVLWEYGGFASEGVKQVSEYGSPVKMEEEIRQKGDDVMTVIKTKAQWPAWQPLNIRAAPSAEFSVDRVRHLMSFLTMLGPSPDWNVGLSGEDLCTRDCGWVQKLVKDLVPWDAGTDSGVSYESPNKPSIPQERIRPLTSLDHPQSPFYDPSGGPMTPLGRVVVERIARKGEQCNTVPDTIDDIVADIAQEE-------KEEGDNTPETCIYSNWSPWSACSSSTCDKGRRMRQRMLKAQLDPNLPCLHTQDFEPCMGPGCSEEEASTCMMSEWISWSPCSASCGM-GMRSRER 485
BLAST of Spondin-1 vs. Ensembl Zebrafish
Match: spon1b (spondin 1b [Source:ZFIN;Acc:ZDB-GENE-000427-10]) HSP 1 Score: 149.828 bits (377), Expect = 1.043e-36 Identity = 152/551 (27.59%), Postives = 240/551 (43.56%), Query Frame = 2
Query: 77 FFILKF----DFAECF-----GFCDIQFPKSYVA---FSTRFNLYTNHEKEKLTKITSYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRER 1663
FIL F D AE GFC I ++ A F L E E +Y P TY +++ + + T+ + E +S D+ F + S T++ CP A + + + + +W AP + C+ + + +I + +CE+ + + C T+ VK C G TA YR+T W+ H KD+P + + + +GASH+ + ++ GG A+EGV QV GS +++++ + G+ +MTVI ++ A N R SA V+ +HL+SFL L PSPDW GLS DLC C W +K + L PWDAG SG +Y + N+ + Q I P+TS +PF G + +G V + I + N ++I+ I+ D A + K + C+ S WS WS+CS S + +++LK + + + T + E C + TC W WSPC+A+C G+ RER
Sbjct: 2 VFILTFLGLQDTAERHSRSEGGFCRILRTQARSARRDGQNEFRLKVEGEPE------TYQPGSTYRVSLYASSPSYFRGFTLIAL-------KEGREGVSPDDYAG----TFKLIDESDTQFMTNCPP-AVTESIPRRRTRIQVFWTAPPSGSGCVTLKASVVQRRIIFFQDEGSLTKRLCEK-----------ESLNEHC-----TNKVKCCACG-TAKYRVTFYGNWSEKLHPKDYPRRANH---WSALIGASHTRSYVLWEYGGFASEGVKQVAEYGSPVKMEEEIRQKGDDVMTVIKTKAQWPAWQPLNIRAAPSAEFSVDRVRHLMSFLTMLGPSPDWNVGLSGEDLCTRDCGWVQKLVKDLVPWDAGTDSGVSYESPNKPSIPQERIRPLTSLDHPQSPFYDPSGGPMTPLGRVVVERIARKGEQCNTVPDTIDDIVADIAQEE-------KEEDNTPETCIYSNWSPWSACSSSTCDKGRRMRQRMLKAQLDPNLPCLHTQDFEPCMGPGCSEEEASTCMMSEWISWSPCSASCGM-GMRSRER 506
BLAST of Spondin-1 vs. Ensembl Xenopus
Match: spon1 (spondin 1, extracellular matrix protein [Source:NCBI gene;Acc:100036727]) HSP 1 Score: 151.369 bits (381), Expect = 5.831e-37 Identity = 127/443 (28.67%), Postives = 202/443 (45.60%), Query Frame = 2
Query: 452 HVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASERCKP 1759
+ W AP I + C+ + + +I + +CE R + P+ C TA YR+T W+ H KD+P + + I +G SHS E+ ++ GG A++GV QV GS +++++ + G+ ++TVI A++ A N R SA V+ ++HL+SFLA + PSPDW GLS DLC C W +K I L PWDAG SG TY + N+ + Q I P+TS +PF G I + V + I N ++++ I+ D + K D C+ S WS WS+CS + K +++LK + + + T + E C + TC W WSPC+A+C G+ RER ++ + + M + + +C + +E C+P
Sbjct: 134 RIQVLWTAPPIGSGCVILKASIVQKKIIYFQDEGSLTKRMCEPDRLGL--------------TGGNEKPIPDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAI---IGGSHSKEYVLWEYGGQASDGVKQVAELGSPVKMEEEIRQKGDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRSRHLMSFLAMMGPSPDWNVGLSSEDLCTKECGWVQKVIQDLIPWDAGTDSGVTYESPNKPSIPQDKIRPLTSLDHPQSPFYDPEGGPIIPVARVVIERIARKGDQCNIVPDNVDDIVPDLVTEE-------KEEDDTPETCIYSNWSPWSACSSATCDKGKRMRQRMLKAQLDLSVPCPDTQDFEPCMGPGCSDDEASTCMMSEWITWSPCSASCGM-GMRSRER-YVKQFPEDGSMCKVPTEETEKC-IVNEECEP 549
BLAST of Spondin-1 vs. Ensembl Xenopus
Match: spon1 (spondin 1, extracellular matrix protein [Source:NCBI gene;Acc:100036727]) HSP 1 Score: 149.828 bits (377), Expect = 1.340e-36 Identity = 145/526 (27.57%), Postives = 229/526 (43.54%), Query Frame = 2
Query: 215 YIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESC-SSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASERCKP 1759
Y P TY +T+ A T+ + E +AG F + T++ CP A + + + W AP I + C+ + + +I + +CE N+ P+ C TA YR+T W+ H KD+P + + I +G SHS E+ ++ GG A++GV QV GS +++++ + G+ ++TVI A++ A N R SA V+ ++HL+SFLA + PSPDW GLS DLC C W +K I L PWDAG SG TY + N+ + Q I P+TS +PF G I + V + I N ++++ I+ D + K D C+ S WS WS+CS + K +++LK + + + T + E C CS S TC W WSPC+A+C G+ RER ++ + + M + + +C + +E C+P
Sbjct: 67 YKPGNTYRVTLLAASPAYFRGFTLIALKEGKEGEKEE---------DHAG--TFQIIDDEDTQFMSNCPV-AVTESTPRRRTRIQVLWTAPPIGSGCVILKASIVQKKIIYFQDEGSLTKRMCEPGGNE--------------------KPIPDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAI---IGGSHSKEYVLWEYGGQASDGVKQVAELGSPVKMEEEIRQKGDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRSRHLMSFLAMMGPSPDWNVGLSSEDLCTKECGWVQKVIQDLIPWDAGTDSGVTYESPNKPSIPQDKIRPLTSLDHPQSPFYDPEGGPIIPVARVVIERIARKGDQCNIVPDNVDDIVPDLVTEE-------KEEDDTPETCIYSNWSPWSACSSATCDKGKRMRQRMLKAQLDLSVPCPDTQDFEPCMGPGCSDDAS--TCMMSEWITWSPCSASCGM-GMRSRER-YVKQFPEDGSMCKVPTEETEKC-IVNEECEP 545
BLAST of Spondin-1 vs. Ensembl Xenopus
Match: spon1 (spondin 1, extracellular matrix protein [Source:NCBI gene;Acc:100036727]) HSP 1 Score: 150.214 bits (378), Expect = 1.367e-36 Identity = 118/375 (31.47%), Postives = 184/375 (49.07%), Query Frame = 2
Query: 656 PVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASERCKP 1759
P+ C TA YR+T W+ H KD+P + + I +G SHS E+ ++ GG A++GV QV GS +++++ + G+ ++TVI A++ A N R SA V+ ++HL+SFLA + PSPDW GLS DLC C W +K I L PWDAG SG TY + N+ + Q I P+TS +PF G I + V + I N ++++ I+ D + K D C+ S WS WS+CS + K +++LK + + + T + E C + TC W WSPC+A+C G+ RER ++ + + M + + +C + +E C+P
Sbjct: 191 PIPDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAI---IGGSHSKEYVLWEYGGQASDGVKQVAELGSPVKMEEEIRQKGDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRSRHLMSFLAMMGPSPDWNVGLSSEDLCTKECGWVQKVIQDLIPWDAGTDSGVTYESPNKPSIPQDKIRPLTSLDHPQSPFYDPEGGPIIPVARVVIERIARKGDQCNIVPDNVDDIVPDLVTEE-------KEEDDTPETCIYSNWSPWSACSSATCDKGKRMRQRMLKAQLDLSVPCPDTQDFEPCMGPGCSDDEASTCMMSEWITWSPCSASCGM-GMRSRER-YVKQFPEDGSMCKVPTEETEKC-IVNEECEP 552
BLAST of Spondin-1 vs. Ensembl Xenopus
Match: spon1 (spondin 1, extracellular matrix protein [Source:NCBI gene;Acc:100036727]) HSP 1 Score: 149.828 bits (377), Expect = 1.490e-36 Identity = 130/444 (29.28%), Postives = 204/444 (45.95%), Query Frame = 2
Query: 452 HVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESC-SSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASERCKP 1759
+ W AP I + C+ + + +I + +CE N+ P+ C TA YR+T W+ H KD+P + + I +G SHS E+ ++ GG A++GV QV GS +++++ + G+ ++TVI A++ A N R SA V+ ++HL+SFLA + PSPDW GLS DLC C W +K I L PWDAG SG TY + N+ + Q I P+TS +PF G I + V + I N ++++ I+ D + K D C+ S WS WS+CS + K +++LK + + + T + E C CS S TC W WSPC+A+C G+ RER ++ + + M + + +C + +E C+P
Sbjct: 121 RIQVLWTAPPIGSGCVILKASIVQKKIIYFQDEGSLTKRMCEPGGNE--------------------KPIPDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAI---IGGSHSKEYVLWEYGGQASDGVKQVAELGSPVKMEEEIRQKGDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRSRHLMSFLAMMGPSPDWNVGLSSEDLCTKECGWVQKVIQDLIPWDAGTDSGVTYESPNKPSIPQDKIRPLTSLDHPQSPFYDPEGGPIIPVARVVIERIARKGDQCNIVPDNVDDIVPDLVTEE-------KEEDDTPETCIYSNWSPWSACSSATCDKGKRMRQRMLKAQLDLSVPCPDTQDFEPCMGPGCSDDAS--TCMMSEWITWSPCSASCGM-GMRSRER-YVKQFPEDGSMCKVPTEETEKC-IVNEECEP 529
BLAST of Spondin-1 vs. Ensembl Xenopus
Match: spon1 (spondin 1, extracellular matrix protein [Source:NCBI gene;Acc:100036727]) HSP 1 Score: 149.058 bits (375), Expect = 3.204e-36 Identity = 121/376 (32.18%), Postives = 186/376 (49.47%), Query Frame = 2
Query: 656 PVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESC-SSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASERCKP 1759
P+ C TA YR+T W+ H KD+P + + I +G SHS E+ ++ GG A++GV QV GS +++++ + G+ ++TVI A++ A N R SA V+ ++HL+SFLA + PSPDW GLS DLC C W +K I L PWDAG SG TY + N+ + Q I P+TS +PF G I + V + I N ++++ I+ D + K D C+ S WS WS+CS + K +++LK + + + T + E C CS S TC W WSPC+A+C G+ RER ++ + + M + + +C + +E C+P
Sbjct: 191 PIPDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAI---IGGSHSKEYVLWEYGGQASDGVKQVAELGSPVKMEEEIRQKGDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRSRHLMSFLAMMGPSPDWNVGLSSEDLCTKECGWVQKVIQDLIPWDAGTDSGVTYESPNKPSIPQDKIRPLTSLDHPQSPFYDPEGGPIIPVARVVIERIARKGDQCNIVPDNVDDIVPDLVTEE-------KEEDDTPETCIYSNWSPWSACSSATCDKGKRMRQRMLKAQLDLSVPCPDTQDFEPCMGPGCSDDAS--TCMMSEWITWSPCSASCGM-GMRSRER-YVKQFPEDGSMCKVPTEETEKC-IVNEECEP 551
BLAST of Spondin-1 vs. Ensembl Mouse
Match: Spon1 (spondin 1, (f-spondin) extracellular matrix protein [Source:MGI Symbol;Acc:MGI:2385287]) HSP 1 Score: 155.606 bits (392), Expect = 1.915e-38 Identity = 145/516 (28.10%), Postives = 224/516 (43.41%), Query Frame = 2
Query: 149 AFSTRFNLYTNHEKEKLTKITSYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTD-PVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRER 1663
A TR YT Y P +Y +T+S + T+ L N + EE +AG F + +T++ CP A + + + +WIAP C+ + + +I + +CEQ + TD P+ C TA YR+T W+ H KD+P + + I +G SHS + ++ GG A+EGV QV GS +++++ + + ++TVI A++ A N R SA V+ T+HL+SFL + PSPDW GLS DLC C W +K + L PWDAG SG TY + N+ T Q I P+TS +PF G I + V + I + N ++++ I+ D A + K D C+ S WS WS+CS S + K +++LK + + + T + + C + TC+ W WSPC+ +C G+ RER
Sbjct: 50 AQGTRREGYTEFSLRVEGDPDFYKPGSSYRVTLSAAPPSYFRGFTL---IALKENQEGDKEE------DHAG--TFQIIDEEETQFMSNCPV-AVTESTPRRRTRIQVFWIAPPTGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQDPT----------------LDGVTDRPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAI---IGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPVNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEE-------KDEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSVSCGM-GMRSRER 526
BLAST of Spondin-1 vs. Ensembl Mouse
Match: Spon2 (spondin 2, extracellular matrix protein [Source:MGI Symbol;Acc:MGI:1923724]) HSP 1 Score: 90.1225 bits (222), Expect = 6.949e-19 Identity = 66/219 (30.14%), Postives = 105/219 (47.95%), Query Frame = 2
Query: 668 CNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKI--TQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDA-SSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPFTVMNGKIKN---IGVVTFQLIQEYNK 1303
C A Y +T KW+ K +P + P + LGA+HS++++++ + G+ G L K+ AG + +V S A S T + S + V+ LVSF+ R+ PSPDW G+ DLC G +W+E+ ++ LYP DAG SG T+ + N T Q + IT+S P + ++K+ I VTF +Q+ +
Sbjct: 34 CTARPLARYSITFIGKWSQTAFPKQYPL---FRPPAQWSSLLGAAHSSDYSMWRKNEYVSNGLRDFAERGEAWALMKEIEAAGEKLQSVHAVFSAPAIPSGTGQTSTELEVHPRHSLVSFVVRIVPSPDWFVGIDSLDLCEGG-RWKEQVVLDLYPHDAGTDSGFTFSSPNFATIPQDTVTEITASSPSHPANSFYYPRLKSLPPIAKVTFVRLQQSPR 248
BLAST of Spondin-1 vs. Ensembl Mouse
Match: Tfpi (tissue factor pathway inhibitor [Source:MGI Symbol;Acc:MGI:1095418]) HSP 1 Score: 70.4774 bits (171), Expect = 5.846e-13 Identity = 39/110 (35.45%), Postives = 58/110 (52.73%), Query Frame = 2
Query: 1826 FYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
++++ + C FIY GC G N F T EEC C P ++T + + E P+ C + G+ + R+ Y+N K+C RF +GGC GN NNF T EC+ +C
Sbjct: 66 YFFNMYTHQCEEFIYGGCEGNENRFDTLEECKKTCIPGYEKTAVKAASGAER-PDFCFLEEDPGL---CRGYMKRYLYNNQTKQCERFVYGGCLGNRNNFETLDECKKIC 171
BLAST of Spondin-1 vs. Ensembl Mouse
Match: Tfpi (tissue factor pathway inhibitor [Source:MGI Symbol;Acc:MGI:1095418]) HSP 1 Score: 70.4774 bits (171), Expect = 5.846e-13 Identity = 39/110 (35.45%), Postives = 58/110 (52.73%), Query Frame = 2
Query: 1826 FYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
++++ + C FIY GC G N F T EEC C P ++T + + E P+ C + G+ + R+ Y+N K+C RF +GGC GN NNF T EC+ +C
Sbjct: 66 YFFNMYTHQCEEFIYGGCEGNENRFDTLEECKKTCIPGYEKTAVKAASGAER-PDFCFLEEDPGL---CRGYMKRYLYNNQTKQCERFVYGGCLGNRNNFETLDECKKIC 171
BLAST of Spondin-1 vs. Ensembl Mouse
Match: Tfpi (tissue factor pathway inhibitor [Source:MGI Symbol;Acc:MGI:1095418]) HSP 1 Score: 70.4774 bits (171), Expect = 5.846e-13 Identity = 39/110 (35.45%), Postives = 58/110 (52.73%), Query Frame = 2
Query: 1826 FYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
++++ + C FIY GC G N F T EEC C P ++T + + E P+ C + G+ + R+ Y+N K+C RF +GGC GN NNF T EC+ +C
Sbjct: 66 YFFNMYTHQCEEFIYGGCEGNENRFDTLEECKKTCIPGYEKTAVKAASGAER-PDFCFLEEDPGL---CRGYMKRYLYNNQTKQCERFVYGGCLGNRNNFETLDECKKIC 171
BLAST of Spondin-1 vs. UniProt/SwissProt
Match: sp|Q8VCC9|SPON1_MOUSE (Spondin-1 OS=Mus musculus OX=10090 GN=Spon1 PE=1 SV=1) HSP 1 Score: 155.606 bits (392), Expect = 1.339e-37 Identity = 145/516 (28.10%), Postives = 224/516 (43.41%), Query Frame = 2
Query: 149 AFSTRFNLYTNHEKEKLTKITSYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTD-PVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRER 1663
A TR YT Y P +Y +T+S + T+ L N + EE +AG F + +T++ CP A + + + +WIAP C+ + + +I + +CEQ + TD P+ C TA YR+T W+ H KD+P + + I +G SHS + ++ GG A+EGV QV GS +++++ + + ++TVI A++ A N R SA V+ T+HL+SFL + PSPDW GLS DLC C W +K + L PWDAG SG TY + N+ T Q I P+TS +PF G I + V + I + N ++++ I+ D A + K D C+ S WS WS+CS S + K +++LK + + + T + + C + TC+ W WSPC+ +C G+ RER
Sbjct: 50 AQGTRREGYTEFSLRVEGDPDFYKPGSSYRVTLSAAPPSYFRGFTL---IALKENQEGDKEE------DHAG--TFQIIDEEETQFMSNCPV-AVTESTPRRRTRIQVFWIAPPTGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQDPT----------------LDGVTDRPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAI---IGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPVNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEE-------KDEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSVSCGM-GMRSRER 526
BLAST of Spondin-1 vs. UniProt/SwissProt
Match: sp|P35446|SPON1_RAT (Spondin-1 OS=Rattus norvegicus OX=10116 GN=Spon1 PE=1 SV=1) HSP 1 Score: 154.836 bits (390), Expect = 2.340e-37 Identity = 145/516 (28.10%), Postives = 224/516 (43.41%), Query Frame = 2
Query: 149 AFSTRFNLYTNHEKEKLTKITSYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTD-PVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDAS-SKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRER 1663
A TR YT Y P +Y +T+S + T+ L N + EE +AG F + +T++ CP A + + + +WIAP C+ + + +I + +CEQ + TD P+ C TA YR+T W+ H KD+P + + I +G SHS + ++ GG A+EGV QV GS +++++ + + ++TVI A++ S N R SA V+ T+HL+SFL + PSPDW GLS DLC C W +K + L PWDAG SG TY + N+ T Q I P+TS +PF G I + V + I + N ++++ I+ D A + K D C+ S WS WS+CS S + K +++LK + + + T + + C + TC+ W WSPC+ +C G+ RER
Sbjct: 50 AQGTRREGYTEFSLRVEGDPDFYKPGSSYRVTLSAAPPSYFRGFTL---IALKENREGDKEE------DHAG--TFQIIDEEETQFMSNCPV-AVTESTPRRRTRIQVFWIAPPTGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQDPT----------------LDGVTDRPILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAI---IGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPSWQPVNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEE-------KDEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSVSCGM-GMRSRER 526
BLAST of Spondin-1 vs. UniProt/SwissProt
Match: sp|Q9HCB6|SPON1_HUMAN (Spondin-1 OS=Homo sapiens OX=9606 GN=SPON1 PE=1 SV=2) HSP 1 Score: 154.836 bits (390), Expect = 2.446e-37 Identity = 146/515 (28.35%), Postives = 224/515 (43.50%), Query Frame = 2
Query: 149 AFSTRFNLYTNHEKEKLTKITSYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRER 1663
A TR YT Y P +Y +T+S + T+ L N + EE +AG F + +T++ CP A + + + +WIAP C+ + + +I + +CEQ ST + K P+ C TA YR+T W+ H KD+P + + I +G SHS + ++ GG A+EGV QV GS +++++ + + ++TVI A++ A N R SA V+ T+HL+SFL + PSPDW GLS DLC C W +K + L PWDAG SG TY + N+ T Q I P+TS +PF G I + V + I + N ++++ I+ D A + K D C+ S WS WS+CS S K +++LK + + + T + + C + TC+ W WSPC+ +C G+ RER
Sbjct: 50 AQGTRREGYTEFSLRVEGDPDFYKPGTSYRVTLSAAPPSYFRGFTL---IALRENREGDKEE------DHAG--TFQIIDEEETQFMSNCPV-AVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQD-------------STFDGVTDK--PILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAI---IGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEE-------KDEDDTPETCIYSNWSPWSACSSSTCDKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSISCGM-GMRSRER 526
BLAST of Spondin-1 vs. UniProt/SwissProt
Match: sp|Q9GLX9|SPON1_BOVIN (Spondin-1 OS=Bos taurus OX=9913 GN=SPON1 PE=1 SV=1) HSP 1 Score: 152.91 bits (385), Expect = 8.986e-37 Identity = 141/493 (28.60%), Postives = 218/493 (44.22%), Query Frame = 2
Query: 215 YIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRER 1663
Y P +Y +T+S + T+ L N + EE +AG F + +T++ CP A + + + +WIAP C+ + + +I + +CEQ ST + K P+ C TA YR+T W+ H KD+P + + I +G SHS + ++ GG A+EGV QV GS +++++ + + ++TVI A++ A N R SA V+ T+HL+SFL + PSPDW GLS DLC C W +K + L PWDAG SG TY + N+ T Q I P+TS +PF G I + V + I + N ++++ I+ D A + K D C+ S WS WS+CS S K +++LK + + + T + + C + TC+ W WSPC+ +C G RER
Sbjct: 72 YKPGTSYRVTLSAAPPSYFRGFTL---IALKENREGDKEE------DHAG--TFQIIDEEETQFMSNCPV-AVTESTPRRRTRIQVFWIAPPAGTGCVILKASIVQKRIIYFQDEGSLTKKLCEQD-------------STFDGVTDK--PILDCCACGTAKYRLTFYGNWSEKTHPKDYPRRANHWSAI---IGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAEDLCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSLDHPQSPFYDPEGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEE-------KDEDDTPETCIYSNWSPWSACSSSTCDKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCTMSEWITWSPCSISCG-TGTRSRER 526
BLAST of Spondin-1 vs. UniProt/SwissProt
Match: sp|Q9W770|SPON1_CHICK (Spondin-1 OS=Gallus gallus OX=9031 GN=SPON1 PE=2 SV=1) HSP 1 Score: 150.984 bits (380), Expect = 3.954e-36 Identity = 141/493 (28.60%), Postives = 215/493 (43.61%), Query Frame = 2
Query: 215 YIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRER 1663
Y P +Y +T+S A T+ ++E E D + F + +T++ CP A + + + +W AP C+ + + +I + ICEQ S S + K P C TA YR+T W+ H KD+P + + I +G+SHS + ++ GG A+EGV QV GS +++++ + + ++TVI A++ A N R SA V+ +HL+SFL L PSPDW GLS DLC C W +K + L PWDAG SG TY + N+ T Q I P+TS +PF G IK + V + I + N ++I+ I+ D A + K D C+ S WS WS+CS S + K +++LK + + + T + + C + TC W WSPC+ +C G RER
Sbjct: 67 YKPGNSYRVTLSAATPAYFRGFTL-----------IALKEGKEGDKEEDHAGTFQIIDEEETQFMSNCPV-AVTESTPRRRTRIQVFWTAPPTGTGCVILKASIVQKRIIYFQDEGSLTKRICEQD-------------SASEGVTDK--PTLDCCACGTAKYRLTFYGNWSEKTHPKDFPRRTNHWSAI---IGSSHSKNYILWEYGGYASEGVKQVAELGSPVKMEEEIRQQSDEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRHRHLMSFLTMLGPSPDWNVGLSAEDLCTKDCGWVQKVVQDLIPWDAGTDSGVTYESPNKPTVPQEKIRPLTSLDHPQSPFYDPEGGSIKLVARVVLERIARKGEQCNFVPDNIDDIVADLAPEE-------KEEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGSTCMMSDWITWSPCSVSCGM-GTRSRER 521
BLAST of Spondin-1 vs. TrEMBL
Match: A0A3P7E8N4 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS2095 PE=4 SV=1) HSP 1 Score: 208.764 bits (530), Expect = 3.661e-52 Identity = 188/633 (29.70%), Postives = 245/633 (38.70%), Query Frame = 2
Query: 581 KRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFY------KAGNGIMTVILARSLDAS---------------SKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQT--PICPITSSWPKDNPFT--------------------------------VMNGKIKNIGVVTFQLIQEYNKNDNQYCNS-INSILQDEAYQNEPKSSALK--------------SNPD----KTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKG-KSEQCIESSLTNTESCSSKCSTVISFETCSFRLWGQWSPCNATCS-QKGIIHRERVFINEQEKHECMQY---------SEMKASLECALASERCKPDVICKEYVYPGDSCSWSSPSQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRR--------FNTPETYPN-------------RCGVSMTWGIFCD-SQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCL 2158
+ N D +Q ++ P C G TA YR+T + WN H +DWP K+ T +GASHS F IY +G A+ GV VC G T L++ + G+ T + + D S + +R+ L VN T L S L L PSPDWCTG+S LC C W ++L+PWDAG+ G TY+ K ++ PI I W NPF +M+G I G ++ K D + S I+ + KS S+P T C+ SEWS WS+CS +CG G KTR R+L+ K E C L ESC + T CSF W WSPCNATC Q G+ R R +E +C S MK C + E C+P IC E G C +RFYY C F Y GC GG N F T EEC LC P ++ R F T + P+ C + G CD + N WYY C F + GC G N + T C C+
Sbjct: 231 RNNPADPIQTPNVDPMELRLRDSIQPKDCCACG-TATYRLTFQGLWNRTTHPQDWPTKNPNLLHWTNLIGASHSPSFQIYAIGQTASAGVQSVCAYGDTTVLREALALAAAKSEGAAGVPTGSQSATTDVSPLRALISTPGMWGEEALMEQRTTLFSVNRTHPLFSLLTMLGPSPDWCTGISGQSLCKADCTWVRNMTLYLHPWDAGIREGNTYMPKESDRKEIPDPIRYIDEKWMPGNPFRDKKPIARVEIERELPKHDWECTNKDADGVHLFLMSGGIHVTGKKASDGMESDKKKDQMHAASATQQIVTGKGRGKSSKSVGFGGPGASGTLGTNNPLSDPSLAQMATFLCITSEWSAWSACSVTCGVGTKTRTRQLIANKKPELCQHVPLVMEESCEGRKRTCDYSAPCSFLPWTLWSPCNATCDRQVGVRSRTRALARPEEAKQCEHLWKGRVGGMESVMKEVEVCRIPPEACEPATICGEGPKQGYPC--GKKVRRFYYSAIDHECLEFEYLGCKGGRNMFETKEECEKLCIPAVEALPGWRRERMGLLQFKTSQLAPSAGDTGEEELKQDPACHYEVNTGYECDPPKELGNYWYYCTRTNRCRDFFYLGCGGTPNRWANHTACMAACM 860
BLAST of Spondin-1 vs. TrEMBL
Match: A0A0R3WMX0 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 PE=4 SV=1) HSP 1 Score: 208.764 bits (530), Expect = 4.014e-52 Identity = 188/633 (29.70%), Postives = 245/633 (38.70%), Query Frame = 2
Query: 581 KRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFY------KAGNGIMTVILARSLDAS---------------SKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQT--PICPITSSWPKDNPFT--------------------------------VMNGKIKNIGVVTFQLIQEYNKNDNQYCNS-INSILQDEAYQNEPKSSALK--------------SNPD----KTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKG-KSEQCIESSLTNTESCSSKCSTVISFETCSFRLWGQWSPCNATCS-QKGIIHRERVFINEQEKHECMQY---------SEMKASLECALASERCKPDVICKEYVYPGDSCSWSSPSQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRR--------FNTPETYPN-------------RCGVSMTWGIFCD-SQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCL 2158
+ N D +Q ++ P C G TA YR+T + WN H +DWP K+ T +GASHS F IY +G A+ GV VC G T L++ + G+ T + + D S + +R+ L VN T L S L L PSPDWCTG+S LC C W ++L+PWDAG+ G TY+ K ++ PI I W NPF +M+G I G ++ K D + S I+ + KS S+P T C+ SEWS WS+CS +CG G KTR R+L+ K E C L ESC + T CSF W WSPCNATC Q G+ R R +E +C S MK C + E C+P IC E G C +RFYY C F Y GC GG N F T EEC LC P ++ R F T + P+ C + G CD + N WYY C F + GC G N + T C C+
Sbjct: 231 RNNPADPIQTPNVDPMELRLRDSIQPKDCCACG-TATYRLTFQGLWNRTTHPQDWPTKNPNLLHWTNLIGASHSPSFQIYAIGQTASAGVQSVCAYGDTTVLREALALAAAKSEGAAGVPTGSQSATTDVSPLRALISTPGMWGEEALMEQRTTLFSVNRTHPLFSLLTMLGPSPDWCTGISGQSLCKADCTWVRNMTLYLHPWDAGIREGNTYMPKESDRKEIPDPIRYIDEKWMPGNPFRDKKPIARVEIERELPKHDWECTNKDADGVHLFLMSGGIHVTGKKASDGMESDKKKDQMHAASATQQIVTGKGRGKSSKSVGFGGPGASGTLGTNNPLSDPSLAQMATFLCITSEWSAWSACSVTCGVGTKTRTRQLIANKKPELCQHVPLVMEESCEGRKRTCDYSAPCSFLPWTLWSPCNATCDRQVGVRSRTRALARPEEAKQCEHLWKGRVGGMESVMKEVEVCRIPPEACEPATICGEGPKQGYPC--GKKVRRFYYSAIDHECLEFEYLGCKGGRNMFETKEECEKLCIPAVEALPGWRRERMGLLQFKTSQLAPSAGDTGEEELKQDPACHYEVNTGYECDPPKELGNYWYYCTRTNRCRDFFYLGCGGTPNRWANHTACMAACM 860
BLAST of Spondin-1 vs. TrEMBL
Match: A0A0R3U498 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS1462 PE=4 SV=2) HSP 1 Score: 207.994 bits (528), Expect = 7.653e-52 Identity = 202/699 (28.90%), Postives = 276/699 (39.48%), Query Frame = 2
Query: 443 QPQHVTFYWIAPKI-PNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAM--------KQKTDPV----------KQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGN------GIMTVILARSLDAS---------------SKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQTP--ICPITSSWPKDNPF----TVMNGKIKNI--------------GVVTFQLIQEY----NKND-------------NQYCNSINSILQDE------AYQNEPKSSALKSN-----PD----KTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGK-SEQCIESSLTNTESCSSKCSTVISFETCSFRLWGQWSPCNATC-SQKGIIHRERVFINEQEKHECMQYSE-----------MKASLECALASERCKPDVICKEYVYPGDSCSWSSPSQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLK---------------ETDQRRFNTPETYPNR------CGVSMTWGIFCD-SQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCL 2158
Q HV W P + C+ I ++K + + + + + + +C + M+ + ++ S + DPV K C TA Y++T + WN H +DWP K T +GASH+ F IY +G A+ GV VC G T L++ A + G+ T + + D S + +RS L VN T LVS L L PSPDWCTG+S LC C W +HLYPWDAG+ G TY+ K R+ P I I SW NPF V KI+ + GV F++ KND Q C +I + Q + S + SN P T C+ EWS WS+CS +CG G KTR R+L+ K E C L ESC + T CS W WSPC TC S +G R+R +E +C E M EC + SE C+P IC E G C +RFYY C F Y GC GG N F T ++C LC P ++ +T Q ++P+ + C + G CD + N WY+ + C F + GC G N F T C C+
Sbjct: 185 QLPHVVAIWRTPSTNTSNCVTIHAMVKSSTSVLK-EVGDLTKTLCPSEMRPMEPVLSENRGSVDADAIDPSREFDRPAPDPVEMRPRDSMQPKDCCACGTATYKLTFQGVWNSTTHPQDWPYKHPNLLHWTNLIGASHAPSFQIYAIGQTASAGVQSVCAYGDTTILREALSLAASKAEGAAGVPTGSQSATTDVSPLRSLITTPGMWGEETLMEERSTLFSVNRTHPLVSMLTMLGPSPDWCTGISGQSLCKADCTWVNNLTLHLYPWDAGIREGNTYMPKESDRREIPDPIRYIDESWMPGNPFRERKPVAVVKIQRVRPKYDWACTNTDEDGVQLFKMGGGIYVTGKKNDKESDESSLKEKAREQRCTTITAGKQKGKRTKSVGFGGPGASGTIGSNNPLSDPSLAQMATFLCITDEWSAWSACSVTCGVGTKTRTRRLIANKKPELCQHVPLVMEESCEGRKRTCDYSAPCSLLPWTLWSPCTGTCDSPRGHRSRKRALARPEEAAQCGHLWEGRNQEGREQGIMSEVEECQVPSETCEPATICGEGPKQGYPCG--PKLRRFYYSAIDHECLEFEYLGCKGGRNMFETKQDCEKLCIPAVEALPAWRRERMGLLQFKTSQLTSSSPDDLEDSLKRDPVCYYEVNTGYECDPPKELGNYWYFCSRTNRCRDFFYLGCGGTPNRFANHTACMAACM 880
BLAST of Spondin-1 vs. TrEMBL
Match: A0A3R7C1T5 (Spondin-1 OS=Clonorchis sinensis OX=79923 GN=SPON1 PE=4 SV=1) HSP 1 Score: 197.208 bits (500), Expect = 2.122e-48 Identity = 99/228 (43.42%), Postives = 140/228 (61.40%), Query Frame = 2
Query: 623 STSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWP----GKDG--YSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFY-KAGNGIMTVILARSLDASSKTNKR--SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETY--LNKNEKTRQT-PICPITSSWPKDNPFTVMNGKIKNIGV 1270
S SCA+++ PV++C +TA+YRM I++ W HW+DWP +DG SP ++ LG SHS ++++H+GG A+ V +C +L+ +F +AG IMTVI R +D+S+ +R SAL VN+T HL+SFL RL PSPDWCTGLSR D+C +C W + L PWDAGV SG TY + +E+ RQ P+CPIT + PFTV+ + G+
Sbjct: 300 SGSCAVQEIQKPVRRCCACNTAIYRMIIQSDWQQQQHWRDWPTAGVNRDGITVSPHFSEILGVSHSPLYDLFHVGGYASPAVDALCTTSDVAKLENEFKNQAGENIMTVIRTRGIDSSAPPEQRIRSALFAVNSTHHLISFLTRLVPSPDWCTGLSRVDICLANCSWPLQMRFRLEPWDAGVMSGNTYVPVEPSERLRQPKPMCPITPDLRANTPFTVVTDYVSPPGI 527 HSP 2 Score: 73.1738 bits (178), Expect = 2.539e-9 Identity = 61/218 (27.98%), Postives = 90/218 (41.28%), Query Frame = 2
Query: 1571 ISFETCSFRLWGQWSPC-NATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASE-RCKPDVICKE-YVYPGDS----CSWSSPSQRFYYDFSSGTCNAFIYK------------GCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCLREK 2167
IS E C WG WS C NATC++ G+I+R R F + K C Y + C + +C P+++ K Y P + + + R+YY S C F Y N F C LC+ + +++ R + + N C C + RWY+D I K+C +F G C N F T+ ECQ C+ E+
Sbjct: 721 ISAEACQSLPWGSWSACINATCTKPGLIYRWREFPDLATKEACESYPLVNELDTCWPPTNLQCDPELLRKACYEEPPSANRLCLRPENTTVRYYYHPSQKECKTFDYSPQCHIRALVSDHAIAYTRNQFPDLASCERLCKFEKQQSRSRWSSRYKRASNPCNNPPYPNSQCGPSETVARWYFDQIKKQCLQFSKGECGSGMNRFATENECQATCVPEQ 938
BLAST of Spondin-1 vs. TrEMBL
Match: H2KTV5 (Spondin-1 OS=Clonorchis sinensis OX=79923 GN=CLF_106333 PE=4 SV=1) HSP 1 Score: 197.593 bits (501), Expect = 2.409e-48 Identity = 99/228 (43.42%), Postives = 140/228 (61.40%), Query Frame = 2
Query: 623 STSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWP----GKDG--YSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFY-KAGNGIMTVILARSLDASSKTNKR--SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETY--LNKNEKTRQT-PICPITSSWPKDNPFTVMNGKIKNIGV 1270
S SCA+++ PV++C +TA+YRM I++ W HW+DWP +DG SP ++ LG SHS ++++H+GG A+ V +C +L+ +F +AG IMTVI R +D+S+ +R SAL VN+T HL+SFL RL PSPDWCTGLSR D+C +C W + L PWDAGV SG TY + +E+ RQ P+CPIT + PFTV+ + G+
Sbjct: 297 SGSCAVQEIQKPVRRCCACNTAIYRMIIQSDWQQQQHWRDWPTAGVNRDGITVSPHFSEILGVSHSPLYDLFHVGGYASPAVDALCTTSDVAKLENEFKNQAGENIMTVIRTRGIDSSAPPEQRIRSALFAVNSTHHLISFLTRLVPSPDWCTGLSRVDICLANCSWPLQMRFRLEPWDAGVMSGNTYVPVEPSERLRQPKPMCPITPDLRANTPFTVVTDYVSPPGI 524 HSP 2 Score: 68.1662 bits (165), Expect = 8.246e-8 Identity = 59/211 (27.96%), Postives = 86/211 (40.76%), Query Frame = 2
Query: 1571 ISFETCSFRLWGQWSPC-NATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASE-RCKPDVICKE-YVYPGDS----CSWSSPSQRFYYDFSSGTCNAFIYK------------GCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQ 2146
IS E C WG WS C NATC++ G+I+R R F + K C Y + C + +C P+++ K Y P + + + R+YY S C F Y N F C LC+ + +++ R + + N C C + RWY+D I K+C +F G C N F T+ ECQ
Sbjct: 718 ISAEACQSLPWGSWSACINATCTKPGLIYRWREFPDLATKEACESYPLVNELDTCWPPTNLQCDPELLRKACYEEPPSANRLCLRPENTTVRYYYHPSQKECKTFDYSPQCHIRALVSDHAIAYTRNQFPDLASCERLCKFEKQQSRSRWSSRYKRASNPCNNPPYPNSQCGPSETVARWYFDQIKKQCLQFSKGECGSGMNRFATENECQ 928
BLAST of Spondin-1 vs. Ensembl Cavefish
Match: spon1b (spondin 1b [Source:ZFIN;Acc:ZDB-GENE-000427-10]) HSP 1 Score: 145.206 bits (365), Expect = 2.558e-35 Identity = 132/494 (26.72%), Postives = 220/494 (44.53%), Query Frame = 2
Query: 212 SYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTR-QTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRER 1663
+Y P TY +++ + + T+ + E +S D+ AG F + +T++ CP A + + + + +W AP + C+ + + +I + +CE +++ + P++ C TA YR+T W+ H KD+P + + + +GASHS + ++ GG A+EGV QV GS +++++ + G+ +MTVI ++ A N R SA V+ HL+SFL L PSPDW G+S +LC C W ++ + L PWDAG SG TY + N+ T Q I P+TS +PF + G I + V + I + CN++ + D P+ + + P+ C+ S WS WS+CS S + +++LK + + + T + E C TC W WSPC+A+C G+ RER
Sbjct: 61 TYQPGSTYRVSLYASSPSYFRGFTLIAL-------KEGREGVSPDDY--AGN--FKIIDEEETQFMTSCPP-AVTESIPRRRTRIQVFWTAPPSGSGCVILKASIVQRRIIFFQDEGSLTRRLCEVQKSIFGGI--------------TLKPLQDCCACGTAKYRVTFYGNWSEKVHPKDYPRRANH---WSALIGASHSRNYVLWEYGGYASEGVRQVAELGSPVKMEEEIRQKGDDVMTVIKTKAQWPAWQPLNIRAAPSAEFSVDRAHHLMSFLTMLGPSPDWNVGISGENLCTQECGWVQRLVKDLLPWDAGTDSGVTYESPNKPTNPQERIRPLTSLDHPQSPFYSPHGGPITPLARVVVERI----ARKGEQCNTVPDTVDDIVADIPPEDTEEDNTPET---CIYSNWSPWSACSSSTCDKGRRMRQRMLKAQLDATVPCLHTQDFEPCMGPGCREEEPSTCMLSEWISWSPCSASCGM-GMRSRER 517
BLAST of Spondin-1 vs. Ensembl Cavefish
Match: spon1a (spondin 1a [Source:ZFIN;Acc:ZDB-GENE-000427-9]) HSP 1 Score: 108.227 bits (269), Expect = 1.035e-23 Identity = 128/492 (26.02%), Postives = 206/492 (41.87%), Query Frame = 2
Query: 215 YIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRER 1663
Y P TY +T+ + A T+ L + E EE AG F + T++ CP A + + + +W AP + CI + + +IS + +CE++ S + P+ C TA YR+T W+ H KD+P + + I +G+SHS + ++ GG A++GV QV GS +++++ + G+ ++TVI ++ A N R SA V+ ++HL+SFL L PSPDW GL + G C W PW++ + S EK R P+TS +PF G I + V + I + N ++++ I+ D A + K D C+ S WS WS+CS S + K +++LK + + + T + E C + TC W WSPC+ +C G+ RER
Sbjct: 78 YQPASTYRVTLYATNPAYFRGFTL---IALKEGRNGEKEE------DYAGN--FQIIDEEDTQFMTNCPP-AVTESTPRRRTRIQVFWTAPPSGSGCILLKASIVQRKIISFQDEGSLTKRMCEKE---------------SLYGESTDKPLLDCCACGTAKYRVTFYGNWSEKLHPKDYPRRANHWSAI---IGSSHSKNYVLWEYGGYASDGVKQVAELGSPVKMEEEIRQKGDEVLTVIKMKAQWPAWQPLNVRAAPSAEFSVDRSRHLMSFLTMLGPSPDWNVGLLSSPV--GRCGW---------PWNS-ICSPNKPTVPQEKIR-----PLTSLDHPQSPFYDPEGGAITQVARVVVERIARKGEQCNIVPDNVDDIVADIAQEE-------KEEDDTPETCIYSNWSPWSACSSSTCEKGKRMRQRMLKAQLDLSVPCPHTQDFEPCMGPGCSDEDATTCMMSEWITWSPCSVSCGL-GMRSRER 514
BLAST of Spondin-1 vs. Ensembl Cavefish
Match: spon2b (spondin 2b, extracellular matrix protein [Source:ZFIN;Acc:ZDB-GENE-990415-161]) HSP 1 Score: 92.8189 bits (229), Expect = 7.527e-20 Identity = 68/203 (33.50%), Postives = 98/203 (48.28%), Query Frame = 2
Query: 632 CAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKI--TQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS----LDASSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKN-EKTRQTPICPITSSWP 1219
C M D V C+ STA Y +T KW+ K +P Y P + +G +HS++++I+ A+ GV + G L K+ AG I +V S LD +S+T V A +SF+ RL PSPDW G+ +LC G W+E + L+P+DAG SG T+ + N E Q + ITSS+P
Sbjct: 41 CTMPVSVDSV--CSAPSTAKYSLTFTGKWSQTAFPKQYPV---YRPPAQWSPLIGVTHSSDYHIWQKNEYASNGVREFSEKGEAWTLIKEVEAAGERIQSVYGLFSTPAVLDGTSQTTTE---FEVYARHSFLSFIMRLVPSPDWFVGVDSLNLCEGD-HWKENISLDLFPYDAGTDSGFTFSSPNFETIPQDKVMQITSSFP 234
BLAST of Spondin-1 vs. Ensembl Cavefish
Match: wfikkn1 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 [Source:NCBI gene;Acc:103037683]) HSP 1 Score: 80.8777 bits (198), Expect = 2.358e-15 Identity = 46/114 (40.35%), Postives = 58/114 (50.88%), Query Frame = 2
Query: 1826 FYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCLREK 2167
++YD S G+C F + GC GG N F TYEEC C QR T P G W RW Y+ INK+C F +GGC+GN NNFR+K EC++ C + K
Sbjct: 335 WHYDTSLGSCRPFAHGGCPGGRNRFETYEECQAAC--------QREGLGLCTLPAVQGPCRNW---------EARWAYNTINKQCQAFVYGGCQGNNNNFRSKVECESRCPQPK 431
BLAST of Spondin-1 vs. Ensembl Cavefish
Match: spon2a (spondin-2-like [Source:NCBI gene;Acc:103039648]) HSP 1 Score: 73.9442 bits (180), Expect = 1.133e-13 Identity = 47/186 (25.27%), Postives = 85/186 (45.70%), Query Frame = 2
Query: 629 SCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKI--TQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDASSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT 1180
S A +T V C+ A Y + W+ K +P + P ++ + +H++++ ++ G A+ GV G EL K+ +A + R+ S S ++++ L+S L ++ PSPDW G+ +LC G +W+++ L+P+DAG SG T+ + N T
Sbjct: 25 SSAFPLRTSNVTTCSARGPASYILVFTGHWSPQTFPKQYPL---FRPPAQWSKLVAVTHNDQYRLWQEGSPASPGVQNFAELGVTVELVKEAKEARKTRAVGSMYRTAGIPSGIGHSSTELILHPRNPLLSLLVKVIPSPDWFVGVDSLNLCEGG-QWKQEVTFDLHPFDAGTDSGFTFSSPNFPT 206
BLAST of Spondin-1 vs. Ensembl Sea Lamprey
Match: spon2b (spondin 2b, extracellular matrix protein [Source:ZFIN;Acc:ZDB-GENE-990415-161]) HSP 1 Score: 75.0998 bits (183), Expect = 8.577e-15 Identity = 62/257 (24.12%), Postives = 110/257 (42.80%), Query Frame = 2
Query: 665 QCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKI--TQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQF---------YKAGNGIMTVILARSLDASSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPFTVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSA-LKSNP 1396
+C+ G Y++ +W+ + K +P + P + + H F ++ A+ GV ++ G + ++ + V A +L + T + S + A Q L+S + RL PSPDW G+ +LC G +W+E+ + L P+DAG SG T+ + N T Q PI +TSS P + K+K + + ++ K + + +++ QNEP S +K P
Sbjct: 9 KCSAGGHPKYQLVFTGRWSQTSFPKQYPL---FRPPAQWSSLVAVVHGRPFQMWRASRFASPGVREMSERGEAWGIMREVDAHAQARTAAARPPAVAGVFSAPALP--NGTGQSSTELEFTAKQPLLSLMVRLVPSPDWFVGVDSLNLCEGE-RWKERLSLDLQPYDAGTDSGFTFSSPNYATIPQEPITQMTSSSPSHPANSFYYPKLKQLPPLARLVLTRMGKRPHSSKHPDANLV---TRQNEPASGVYIKQTP 256
BLAST of Spondin-1 vs. Ensembl Sea Lamprey
Match: spon2a (spondin 2a, extracellular matrix protein [Source:ZFIN;Acc:ZDB-GENE-990415-160]) HSP 1 Score: 60.077 bits (144), Expect = 1.510e-10 Identity = 39/132 (29.55%), Postives = 58/132 (43.94%), Query Frame = 2
Query: 1010 VSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTR-QTPICPITS---SWPKDNPFTVMNGKIKNIGVVTFQL---IQEYNKNDNQYCNSINSILQDEAYQNEPKSSAL 1384
VS + RL PSPDW G+S DLC+ + W L PWD G SG + + N + Q PI IT+ S P ++ + + IG + F L + + D+ C + D + E + L
Sbjct: 1 VSIVVRLIPSPDWFVGVSSLDLCSNAGGWAPLVSHDLQPWDGGTDSGFAFSSPNYASEPQEPISLITAQRPSHPANSFYYPRLQALPRIGFLEFHLQPADHAFQRPDDLICKHCQIVRSDSGQREEAAGTPL 132
BLAST of Spondin-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005038.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005038.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 60.077 bits (144), Expect = 2.844e-10 Identity = 33/112 (29.46%), Postives = 52/112 (46.43%), Query Frame = 2
Query: 1823 RFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCL 2158
R +Y+ + C F Y GC+G NNF + EC C Q R + P C + M G+ ++ R++++ + C F +GGC+GN NNF+ C C+
Sbjct: 18 RVFYNRYTQRCEPFHYGGCFGNGNNFESLAECNAKCAWIPDHRLQLRRKQTDIAPKACRLDMEVGV---CRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHCV 126 HSP 2 Score: 53.5286 bits (127), Expect = 4.596e-8 Identity = 36/129 (27.91%), Postives = 60/129 (46.51%), Query Frame = 2
Query: 1820 QRFYYDFSSGTCNAFIYKGCYGGLNNFHT----YEECILLCRP-KLKETDQRRFNTPETYPNRCG------------VSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
+R++++ +SG+C F Y GC G LNNF Y+ C+ P K+K+ ++ N RC + + + + R++Y+ C F +GGC GN NNF ++ C +VC
Sbjct: 89 RRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHCVHRAVPLKIKKRSKQTNNNNNNKAARCNGESNSEQFVLPRICLMKADPGECTAVIPRFHYNPKTSNCESFTYGGCGGNDNNFSSERSCMSVC 217
BLAST of Spondin-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009465.1 (pep scaffold:Pmarinus_7.0:GL478507:22936:25713:-1 gene:ENSPMAG00000008558.1 transcript:ENSPMAT00000009465.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 60.4622 bits (145), Expect = 3.019e-10 Identity = 42/115 (36.52%), Postives = 56/115 (48.70%), Query Frame = 2
Query: 1823 RFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLC-RPKLKETDQ-RRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCLR 2161
R+ YD ++ TC F Y GC G NNF EC++ C R +K +F + +P C + G + S RW YD + C F +GGC GN NNF +TEC C R
Sbjct: 16 RWTYDAATSTCKEFTYGGCRGNQNNFLEEAECLMDCGRSDVKMAVYCWQFGS---WPYICLLEEAVG---PCRGSFTRWIYDAESSNCKEFTYGGCGGNFNNFLEETECMVTCGR 124 HSP 2 Score: 58.9214 bits (141), Expect = 8.793e-10 Identity = 42/130 (32.31%), Postives = 54/130 (41.54%), Query Frame = 2
Query: 1823 RFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPETYP---NRCG-VSMTW---------------GIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
R+ YD S C F Y GC G NNF EC++ C L R++ Y N C +S W + + S RW YD + C F +GGC GN NNF +TEC +C
Sbjct: 87 RWIYDAESSNCKEFTYGGCGGNFNNFLEETECMVTCGRSLGTLRTCRYHRTLNYLVVNNSCFLISSCWMTRRLFHVQDVCLLEAVTSPCRGSFTRWTYDAESGHCKEFTYGGCGGNFNNFLEETECVAMC 216
BLAST of Spondin-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005043.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005043.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 58.5362 bits (140), Expect = 9.992e-10 Identity = 34/115 (29.57%), Postives = 54/115 (46.96%), Query Frame = 2
Query: 1823 RFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRRFNTP--ETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCLR 2161
R +Y+ + C F Y GC+G NNF + EC C D +R +P P C + M G+ ++ R++++ + C F +GGC+GN NNF+ C C+
Sbjct: 18 RVFYNRYTQRCEPFHYGGCFGNGNNFESLAECNAKC---AWIPDNKRLASPCPLVAPKACRLDMEVGV---CRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHCVH 126 HSP 2 Score: 53.5286 bits (127), Expect = 4.935e-8 Identity = 36/129 (27.91%), Postives = 60/129 (46.51%), Query Frame = 2
Query: 1820 QRFYYDFSSGTCNAFIYKGCYGGLNNFHT----YEECILLCRP-KLKETDQRRFNTPETYPNRCG------------VSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
+R++++ +SG+C F Y GC G LNNF Y+ C+ P K+K+ ++ N RC + + + + R++Y+ C F +GGC GN NNF ++ C +VC
Sbjct: 88 RRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHCVHRAVPLKIKKRSKQTNNNNNNKAARCNGESNSEQFVLPRICLMKADPGECTAVIPRFHYNPKTSNCESFTYGGCGGNDNNFSSERSCMSVC 216
BLAST of Spondin-1 vs. Ensembl Nematostella
Match: EDO49895 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RF43]) HSP 1 Score: 106.301 bits (264), Expect = 1.063e-24 Identity = 101/370 (27.30%), Postives = 171/370 (46.22%), Query Frame = 2
Query: 665 QCNIGSTAVYRMTIENKWNLLNHWKDWPGKDG-YSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDASSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEK-TRQTPICPITSSW---PKDNPFTVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIE-SSLTNTESCS-SKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASERC 1753
+C A Y++T + KW+ H + PG +SP I G SHSN++ ++ GG+A+ V + G+ L ++ +M++I SL + SA+ V++T+HL+S ++R+ PSPDW G+ R +LC +C W+ + + L PWDAG G T+ + N + T + PI IT+ P+ + + N +I+ + + + C++ I Y PKS+ + C L+ WS+W+SCS +CG GE+ R R +++ I +L C+ +CS S + C W WS C +C + R+ Q M + +C L+ C
Sbjct: 1 ECCACGKATYKLTFKGKWSQETHARHHPGPMARWSPLI----GCSHSNKYVMWRYGGIASRAVQMMSEWGATEYLDEEMRNQQENVMSIIQTPSLWYG--VGESSAVFRVDSTRHLLSMISRMIPSPDWNVGVDRVNLCTTNCTWKHEETIDLIPWDAGTDDGITFKSPNARSTPKQPISRITTRLHDHPRASFYLNTNDQIRPFASLKLERLAVSGG-----CSNDAHIAPKNPY--PPKSARID--------CQLTHWSKWTSCSVTCGVGEQIRGRDIIRVPEHDGIPCPNLYEHRPCARQRCS---STKKCEVTPWSAWSSCRGSCGVGRKVRTRRII--RQSSLSGMTCPSLVKKRKCKLSPCHC 344
BLAST of Spondin-1 vs. Ensembl Nematostella
Match: EDO31760 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SX09]) HSP 1 Score: 59.3066 bits (142), Expect = 2.405e-11 Identity = 25/45 (55.56%), Postives = 34/45 (75.56%), Query Frame = 2
Query: 2033 QSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCLREK 2167
++S RWYY+N ++C +F FGGC GNANNF TK+ C+N C+R K
Sbjct: 11 RASIQRWYYNNETRKCIKFTFGGCHGNANNFPTKSSCRNACMRRK 55
BLAST of Spondin-1 vs. Ensembl Nematostella
Match: EDO38466 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCV3]) HSP 1 Score: 46.9802 bits (110), Expect = 7.205e-7 Identity = 19/36 (52.78%), Postives = 24/36 (66.67%), Query Frame = 2
Query: 2048 RWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
RW+YD + EC F +GGC+GNANNF +K C C
Sbjct: 30 RWHYDMRSSECKMFTYGGCQGNANNFESKAACLKKC 65
BLAST of Spondin-1 vs. Ensembl Nematostella
Match: EDO41696 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1]) HSP 1 Score: 46.2098 bits (108), Expect = 7.331e-7 Identity = 17/36 (47.22%), Postives = 28/36 (77.78%), Query Frame = 2
Query: 2048 RWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
RW+Y+ +++C +F +GGC+GN+NNF +K EC+ C
Sbjct: 17 RWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEKKC 52
BLAST of Spondin-1 vs. Ensembl Nematostella
Match: EDO26263 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1]) HSP 1 Score: 46.2098 bits (108), Expect = 7.331e-7 Identity = 17/36 (47.22%), Postives = 28/36 (77.78%), Query Frame = 2
Query: 2048 RWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
RW+Y+ +++C +F +GGC+GN+NNF +K EC+ C
Sbjct: 17 RWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEKKC 52
BLAST of Spondin-1 vs. Ensembl Medaka
Match: spon1b (spondin-1 [Source:NCBI gene;Acc:101161803]) HSP 1 Score: 148.288 bits (373), Expect = 2.552e-36 Identity = 139/532 (26.13%), Postives = 232/532 (43.61%), Query Frame = 2
Query: 113 GFCDIQFPKSYVAFSTR-----FNLYTNHEKEKLTKITSYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRER 1663
GFC+ + P++ + + + F L E+E +Y P TY + + A T+ ++E +E + F + T++ CP A + + + +W AP + C+ + + +I + +CE++ ++D A P++ C G TA YR+T W+ H KD+P + + + +GASHS + ++ GG A+EGV QV GS +++ + + G+ ++TVI ++ A N R SA V+ +HL+SFL L PSPDW GLS DLC C W ++ + L PWDAG SG TY + N+ T Q I P+TS +PF G I + V + I + N ++I+ I+ D + K D C+ S WS WS+CS + + +++LK + + + T + + C + ++ TC W WS C+ +C G+ RER
Sbjct: 32 GFCE-RIPRAQIQRTRKEGHHEFRLKVEGEQE------NYNPGTTYKVLLLATSPAYFRGFTL-----------IALKEGTEGTTPDDYIGQFQIVDKDDTQFMTSCPP-AVTETTPRRRTKIQVFWTAPPSGSGCVILKASIVQKRVIFFQDKGSLTVRLCEKEPVQLDTTSAP--------------PIECCACG-TAKYRLTFYGNWSEKVHPKDYPRRANH---WSALIGASHSRNYVLWEYGGYASEGVRQVAEFGSPIKMEGEIRQKGDDVLTVIKTKAQWPAWQPLNLRAAPSAEFSVDRARHLMSFLTMLGPSPDWNVGLSGEDLCTKDCGWIQRLVADLIPWDAGTDSGVTYESPNKPTIPQDKIRPLTSFDHPQSPFYDPQGGPISPLARVVVERIARKGEQCNVVPDNIDDIVADIGQEE-------KEEDDTPETCIYSAWSPWSACSSATCDKGRRMRQRMLKAQLDLSVPCPHTQDFQPCMAPGCSMEEPSTCMMSEWISWSACSVSCGM-GMRSRER 518
BLAST of Spondin-1 vs. Ensembl Medaka
Match: spon1a (spondin 1 [Source:NCBI gene;Acc:101161963]) HSP 1 Score: 142.895 bits (359), Expect = 1.696e-34 Identity = 132/494 (26.72%), Postives = 216/494 (43.72%), Query Frame = 2
Query: 212 SYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEY---CPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARS-LDASSKTNKR---SALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKT-RQTPICPITSSWPKDNPF-TVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLT-NTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRER 1663
SY P TY + V + + T+ ++E + D + F + +T++ CP A + + ++ +W AP + C+ + + +I + +CE++ D P+ C TA YR+T W+ H KD+P + + I +GASHS + ++ GG A++GV QV GS +++++ + G+ ++TVI ++ A N R SA V+ +HL+SFL + PSPDW GLS +LC C W +K + L PWDAG SG +Y + N+ T Q I P+TS +PF G I + V + I + N ++++ I+ D A + K D C+ S WS WS+CS + + K +++LK + + + T + E C + TC W WSPC+ +C GI RER
Sbjct: 69 SYQPGSTYRVNVIASSPSYFRGFTL-----------IALKEGAGGDKDDDYAGNFRIIDEDETQFMTNCPH-AVTESTPRRRNNIQVFWTAPPTGSGCVLLKASIVQKKIIFFQDEGPLTKQMCEKESLYGDATDK---------------PLLDCCACGTAKYRVTFYGNWSEKIHPKDYPRRANHWSAI---IGASHSKNYVLWEYGGSASDGVKQVAELGSPVKMEEEIRQKGDDVLTVIKMKAQWPAWQPLNVRAAPSAEFSVDRIRHLMSFLTMMGPSPDWNVGLSAENLCTKECGWVQKVVQDLIPWDAGTDSGVSYESSNKPTVPQEKIRPLTSLDHPQSPFYDPEGGAITPVARVVVERIARKGEQCNIVPDNVDDIVADIAQEE-------KEEDDTPETCIYSNWSPWSACSSATCEKGKRMRQRMLKAQLDLSVPCPHTQDFEPCMGPGCSDEDGSTCMMSEWITWSPCSMSCG-AGIRSRER 524
BLAST of Spondin-1 vs. Ensembl Medaka
Match: spon2b (spondin 2 [Source:NCBI gene;Acc:101164320]) HSP 1 Score: 91.2781 bits (225), Expect = 2.034e-19 Identity = 91/374 (24.33%), Postives = 137/374 (36.63%), Query Frame = 2
Query: 659 VKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKI--TQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDAS-SKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKN-EKTRQTPICPITSSWPKDNPFTVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLTNTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASE--RCKPD 1762
+ C A Y +T KW K +P Y P + +G +HS +++++ A+ GV + L K+ AG I +V S A T + V A L+SF+ R+ PSPDW G+ +LCNG KW+E + L+P+DAG SG T+ + N E Q I ITSS+P + ++K++ + + + K N I S+ + N LL +E IE +L NT C +W W C C + G+ HR R I + + C L E +C PD
Sbjct: 37 LPMCTASEPAQYSLTFSGKWTRAAFPKQYPV---YRPPAQWSNLIGVTHSLDYHMWQRNEFASNGVREFAEKSEAWTLMKEVETAGERIQSVYGILSAPAVVGGTGQMDTEFEVFARHSLMSFMVRIIPSPDWFVGVDSINLCNGD-KWKESVTLELFPYDAGTDSGFTFSSPNFETIPQDRITQITSSYPSHPANSFFYPRLKHLPPIAKVKLTKIKKT-----NQIISLPMEPTQSN------------------------------------------LLPTGNE--IEETLINTPL------------DCEVSVWSPWGLCKGKCGESGVQHRTRYVIMHPANN----------GVACPLLEEERKCIPD 335
BLAST of Spondin-1 vs. Ensembl Medaka
Match: spon2a (spondin-2 [Source:NCBI gene;Acc:101163419]) HSP 1 Score: 71.633 bits (174), Expect = 7.079e-13 Identity = 55/231 (23.81%), Postives = 101/231 (43.72%), Query Frame = 2
Query: 665 QCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKI--TQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDASSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQTP-ICPITSSWPKDNPFTVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQD 1348
+C S A Y + W+ + K +P + P ++ + SH++ F ++ G A+ GV + G EL K +A + R+ + S + + L+S + ++ PSPDW G++ +LC G+ W+++ V+L P+DAG SG T+ + T I ITS P + ++K + + I +++ N+ N IL +
Sbjct: 37 ECTAKSPASYILVFTGHWSPQSFPKQYPL---FRPPAQWSKLIAVSHNHHFRLWEEGAAASLGVQKFAELGLTVELMKAGKEARKKRTVGAMQRTAGIPNGIGHSSTEVHMQPRSSLLSLMVKMIPSPDWFVGVNSLNLCEGN-HWKQEVTVNLQPYDAGTNSGFTFSSPKFPTSPPENISKITSQVPNHPANSFYYPRLKELPAIASIKITRQHRSANRQTVLSNHILPN 263
BLAST of Spondin-1 vs. Ensembl Medaka
Match: COL6A3 (collagen type VI alpha 3 chain [Source:NCBI gene;Acc:101161016]) HSP 1 Score: 71.633 bits (174), Expect = 3.386e-12 Identity = 42/150 (28.00%), Postives = 63/150 (42.00%), Query Frame = 2
Query: 1757 PDVICKEYVYPGDSCSWSSPSQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLC-----------------RPKLKETDQRRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
P + CK+Y ++YY+ +G C F Y GC G N F + C+ C +P+ ++T+Q P + C + G + +WYYD +K C RF +GGC GN N F T+ EC+ C
Sbjct: 2557 PGMPCKDY------------QAKWYYERQNGFCTQFWYGGCGGNDNRFDSEALCLKRCLRSEVQLAGKPEEKPQMQPEAQQTEQ--VEIPPAPVDICQLPKQEG---SCAEFALKWYYDTTSKSCTRFWYGGCDGNQNRFDTQEECKEAC 2689
BLAST of Spondin-1 vs. Planmine SMEST
Match: SMESG000039905.1 (SMESG000039905.1) HSP 1 Score: 1621.68 bits (4198), Expect = 0.000e+0 Identity = 834/837 (99.64%), Postives = 835/837 (99.76%), Query Frame = 2
Query: 47 MKLILNILSTFFILKFDFAECFGFCDIQFPKSYVAFSTRFNLYTNHEKEKLTKITSYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEYCPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDASSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQTPICPITSSWPKDNPFTVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLKGKSEQCIESSLTNTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASERCKPDVICKEYVYPGDSCSWSSPSQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCLREKXXXXXXXXISQPVTVRKVCIYXXXXXXXXXXXXXQNQQTRIRFLIEKNDDCTESISETRKCQSEKCNSPNNGFISLRNCKYXXXXXXXXXXXRCGPGVRERKRKKLSRNCKPEFPQFTDRESCYNACAYD 2557
MKLILNILSTFFILKFDFAECFGFCDIQFPKSYVAFSTRFNLYTNHEKEKLTKITSYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEYCPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDASSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQTPICPITSSWPKDNPFTVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLSEWSEWSSCSESCGKGEKTRYRKLLKGKSEQCIESSLTNTESCSSKCST ISFETCSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASERCKPDVICKEYVYPGDSCSWSSPSQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPETYPNRCGVSMTWGIFCDSQS SNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCLREKSTSTTTTTISQPVTVRKVCIYSNWSDWSSCD+NCQNQQTRIRFLIEKNDDCTESISETRKCQSEKCNSPNNGFISLRNCKYSSWSEWSACSSRCGPGVRERKRKKLSRNCKPEFPQFTDRESCYNACAYD
Sbjct: 1 MKLILNILSTFFILKFDFAECFGFCDIQFPKSYVAFSTRFNLYTNHEKEKLTKITSYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEYCPQRAYVQYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDASSKTNKRSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQTPICPITSSWPKDNPFTVMNGKIKNIGVVTFQLIQEYNKNDNQYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLSEWSEWSSCSESCGKGEKTRYRKLLKGKSEQCIESSLTNTESCSSKCSTAISFETCSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASLECALASERCKPDVICKEYVYPGDSCSWSSPSQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPETYPNRCGVSMTWGIFCDSQSPSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCLREKSTSTTTTTISQPVTVRKVCIYSNWSDWSSCDDNCQNQQTRIRFLIEKNDDCTESISETRKCQSEKCNSPNNGFISLRNCKYSSWSEWSACSSRCGPGVRERKRKKLSRNCKPEFPQFTDRESCYNACAYD 837
BLAST of Spondin-1 vs. Planmine SMEST
Match: SMESG000009946.1 (SMESG000009946.1) HSP 1 Score: 246.128 bits (627), Expect = 3.387e-69 Identity = 212/747 (28.38%), Postives = 322/747 (43.11%), Query Frame = 2
Query: 149 AFSTRFNLYTNHEKEKLTKITSYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSN--------------VHTEMEE---LSEHDW---KNAGEVVFNVTHASKTEYCPQRAYV---QYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDASSKTNK------RSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQT--PICPITSSWPKDNPFT-------VMNGKIK--------NIGVVTFQLIQEYNKNDN---QYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLK-GKSEQCIESSLTNTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASL----ECALASERCKPDVICKEYVYPGDSCSWSSPSQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQ------------------RRFNTPETYPNRCGVSMTW-GIFCDSQSSSN-RWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCLREK 2167
A +TR N + H+K T + D + I + M ++ E Y ++ N EME+ + H + G + N CP R + +L +V WIAP P CIR + D + IC K KM + ++ S + T VK+C A Y++T W+ H KDWP K T +GA+H+ ++ IY+ GG A++ V +C G T L++QF + + L + S N+ RS I VN T H SFL + PSPDWCTG++ D+C C W++ F + L+P+DAG+ +G TY +N + PI I +++ PFT V+ KIK + + E N N N Q+ S + + + P +N T C+ S WSEW+ CS +CG G + R R +LK K+E C L + +C + + CS W +W PCNATC G R R+F +EK +C++ E + + C + C P +IC E + G SC P +++YD + +C F Y GC G LNNF + C C + Q +R P T N+C + + + G+ CD + R+YYDN N C +F++ GC GN+NNFR+ C C+ ++
Sbjct: 7 AMNTRTNCFKMHKK---TYQKVMMKDGEFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFL----TYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMIS-EENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWP-KQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEE---TIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANM-ATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSC--GKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMP-TMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQ 737
BLAST of Spondin-1 vs. Planmine SMEST
Match: SMESG000009946.1 (SMESG000009946.1) HSP 1 Score: 245.358 bits (625), Expect = 6.979e-69 Identity = 212/747 (28.38%), Postives = 322/747 (43.11%), Query Frame = 2
Query: 149 AFSTRFNLYTNHEKEKLTKITSYIPDKTYDITVSLNDKAMHEKLTIKEIYVLVSN--------------VHTEMEE---LSEHDW---KNAGEVVFNVTHASKTEYCPQRAYV---QYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDASSKTNK------RSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQT--PICPITSSWPKDNPFT-------VMNGKIK--------NIGVVTFQLIQEYNKNDN---QYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLK-GKSEQCIESSLTNTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASL----ECALASERCKPDVICKEYVYPGDSCSWSSPSQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQ------------------RRFNTPETYPNRCGVSMTW-GIFCDSQSSSN-RWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCLREK 2167
A +TR N + H+K T + D + I + M ++ E Y ++ N EME+ + H + G + N CP R + +L +V WIAP P CIR + D + IC K KM + ++ S + T VK+C A Y++T W+ H KDWP K T +GA+H+ ++ IY+ GG A++ V +C G T L++QF + + L + S N+ RS I VN T H SFL + PSPDWCTG++ D+C C W++ F + L+P+DAG+ +G TY +N + PI I +++ PFT V+ KIK + + E N N N Q+ S + + + P +N T C+ S WSEW+ CS +CG G + R R +LK K+E C L + +C + + CS W +W PCNATC G R R+F +EK +C++ E + + C + C P +IC E + G SC P +++YD + +C F Y GC G LNNF + C C + Q +R P T N+C + + + G+ CD + R+YYDN N C +F++ GC GN+NNFR+ C C+ ++
Sbjct: 14 AMNTRTNCFKMHKK---TYQKVMMKDGEFRIVIRDMKGKMADRFEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDEGLGHFIINKKFGLVDYKCPNRMHGNDNMFL----TYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMIS-EENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWP-KQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEE---TIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANM-ATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSC--GKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMP-TMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQ 744
BLAST of Spondin-1 vs. Planmine SMEST
Match: SMESG000009946.1 (SMESG000009946.1) HSP 1 Score: 244.202 bits (622), Expect = 1.109e-68 Identity = 202/706 (28.61%), Postives = 311/706 (44.05%), Query Frame = 2
Query: 215 YIPDKTYDITVSLNDKAMH-EKLTIKEIYVLVSNVHTEMEELSEHDWKNAGEVVFNVTHASKTEYCPQRAYV---QYLYTQPQHVTFYWIAPKIPNFCIRITVILKMNDLISESKFEERSHWICEQKRNKMDQMQADQYASTSCAMKQKTDPVKQCNIGSTAVYRMTIENKWNLLNHWKDWPGKDGYSPKITQYLGASHSNEFNIYHLGGMANEGVSQVCGNGSYTELQKQFYKAGNGIMTVILARSLDASSKTNK------RSALIVVNATQHLVSFLARLHPSPDWCTGLSRFDLCNGSCKWQEKFIVHLYPWDAGVYSGETYLNKNEKTRQT--PICPITSSWPKDNPFT-------VMNGKIK--------NIGVVTFQLIQEYNKNDN---QYCNSINSILQDEAYQNEPKSSALKSNPDKTRHCLLXXXXXXXXXXXXXXXXXXTRYRKLLK-GKSEQCIESSLTNTESCSSKCSTVISFETCSFRLWGQWSPCNATCSQKGIIHRERVFINEQEKHECMQYSEMKASL----ECALASERCKPDVICKEYVYPGDSCSWSSPSQRFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQ------------------RRFNTPETYPNRCGVSMTW-GIFCDSQSSSN-RWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVCLREK 2167
+ P ++Y I ++ M +I Y + ++ E ++ + G + N CP R + +L +V WIAP P CIR + D + IC K KM + ++ S + T VK+C A Y++T W+ H KDWP K T +GA+H+ ++ IY+ GG A++ V +C G T L++QF + + L + S N+ RS I VN T H SFL + PSPDWCTG++ D+C C W++ F + L+P+DAG+ +G TY +N + PI I +++ PFT V+ KIK + + E N N N Q+ S + + + P +N T C+ S WSEW+ CS +CG G + R R +LK K+E C L + +C + + CS W +W PCNATC G R R+F +EK +C++ E + + C + C P +IC E + G SC P +++YD + +C F Y GC G LNNF + C C + Q +R P T N+C + + + G+ CD + R+YYDN N C +F++ GC GN+NNFR+ C C+ ++
Sbjct: 31 FEPGESYRIILNNAMYGMDFRDFSIFAAYEMEDSLQMESHKMIPDE--GLGHFIINKKFGLVDYKCPNRMHGNDNMFL----TYVESTWIAPHNPINCIRFEAQVVKFDFMVYESIGRLVKVICPTKMYKMIS-EENKNMKISKPFDEMTPMVKECCACGVASYKVTFMGLWSKKTHPKDWP-KQKSMVHWTNLIGATHTGDYFIYYNGGQASQAVQSICSYGDSTVLKQQFAEE---TIKPHLKSYFNTSGMWNEDEIEQSRSGFISVNRTHHFFSFLTMMGPSPDWCTGVAAVDMCMNDCTWKDDFTMDLFPFDAGIKNGFTYFPENSDRQDIPDPIRTINTTYMTQFPFTANVPVARVIYQKIKPKHNWECTKMNAKQVKEFDEMNSNKNGNSQFDGSSLTKKRRHINKKSPLDDPTLANM-ATFLCITSPWSEWTKCSVTCGIGTEMRSRDMLKNAKTELCRHLPLLESRTCEGRKRSCDFSAPCSLLSWSRWGPCNATCEMHGYQQRSRMFARFEEKEKCLKNKERRFKIFEEKRCKKDTALCDPVIICSEGLIMGSSC--GKPVMKYFYDAAKHSCVPFEYLGCKGSLNNFPSKMSCEKTCLAAVNALPQWRKDKMAHLQFQSQSNSDKRKKMP-TMANKCRLEVKYGGMNCDGTMGPDIRYYYDNRNGRCRQFKYTGCDGNSNNFRSYHSCIKACMPDQ 721
BLAST of Spondin-1 vs. Planmine SMEST
Match: SMESG000037996.1 (SMESG000037996.1) HSP 1 Score: 60.077 bits (144), Expect = 2.186e-10 Identity = 36/111 (32.43%), Postives = 58/111 (52.25%), Query Frame = 2
Query: 1823 RFYYDFSSGTCNAFIYKGCYGGLNNFHTYEECILLCRPKLKETDQRRFNTPETYPNRCGVSMTWGIFCDSQSSSNRWYYDNINKECYRFRFGGCRGNANNFRTKTECQNVC 2155
R++Y+ +S C FIY GC NNF T ++C LC + K+ C + G FC ++ R++Y++ +++C RF +GGC N NNF+ +CQ+ C
Sbjct: 42 RYFYNDTSEKCERFIYGGCGANGNNFKTLKDCQDLCEERNKQN--------------CEDPLAKG-FC--KAYIPRYFYNDTSEKCERFIYGGCGANGNNFKILKDCQDFC 135 The following BLAST results are available for this feature:
BLAST of Spondin-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Spondin-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Spondin-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Spondin-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Spondin-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Spondin-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30002168 ID=SMED30002168|Name=Spondin-1|organism=Schmidtea mediterranea sexual|type=transcript|length=2693bpback to top protein sequence of SMED30002168-orf-1 >SMED30002168-orf-1 ID=SMED30002168-orf-1|Name=SMED30002168-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=838bp MKLILNILSTFFILKFDFAECFGFCDIQFPKSYVAFSTRFNLYTNHEKEKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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