SMED30002155

Overview
NameSMED30002155
Smed IDSMED30002155
Length (bp)6793
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30002155 (SMED30002155) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30002155 (SMED30002155) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30002155 (SMED30002155) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30002155 (SMED30002155) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30002155

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
neuronSMED30002155SMESG000074740.1 SMESG000074739.1 dd_Smed_v4_8417_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30002155SMESG000074740.1 SMESG000074739.1 dd_Smed_v4_8417_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30002155SMESG000074740.1 SMESG000074739.1 dd_Smed_v6_8417_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30002155 vs. Planmine SMEST
Match: SMESG000074739.1 (SMESG000074739.1)

HSP 1 Score: 3256.08 bits (8441), Expect = 0.000e+0
Identity = 1700/1706 (99.65%), Postives = 1701/1706 (99.71%), Query Frame = 1
Query: 1489 MNKNSKKAKPLSNKTSENNTNSVNPTETPILNSPAQIQCKTKPFSDSRIRIKKDHQIQEPSYSNKRTLKKFSTKPMSVGINQPEIMLKSYSDDLNTNIFKSLSETSVRLLTEIISNINKTNQILTSRNTEKSQTENRDYTPLVSEYLQYLMGINDMDFRNENCRQHPIYDMLQELQFNAMKITEKQAILNKSNNGLSQTLERFYQEQLNKCYDDIKRLKRQLESTQQLLTSKMPADATQVLFEAKQKLQAELESVDREYARRMSRERKRLEDSENLYNELLVDHENMQKKYISNINSLNQQIEQLERKIEADRRFIDQQQFEWELERSQHNVDMENLNRSIGRIKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXSKQRSFSVELTEISCNEIATQTIQNDVIILKAHLVFADLEKYDEFQDLKEEVVNSFMLLINFIHLLVDLIFDVTEKNKLNVSLKILHEFNSKQSCNIIFQLDHLQWDCMREEILNFRDFLNNFINEQANLKHNCDRIQEDLQISATDMALKQDIITSLGEEIQSLMEFQRNLSKDYDILQSMLEEKQNELDNLSAKLLSQQTIDEYEDTIQNLNKEIIEKQNIIDERDEDLYVLKEDKELMEQELKSSKEKYEAIIIQHNDIYQDLEKEMKDCQSLNHEFQDQINNYLIKIREQDEEISNINEKQISLIDQISELTEKNDILIHKNLDLNDETKLTELRLSEMKEQHKLEVLKINTSLEEIEKFNRELMEKTKIKQGIFESASCQTDRDNNESMCRDSLMSCVNIQDKLLTTIDKLKREYVREKELRQQIEIKYKHFAAQYETLSSKVILMGNKVNETRTYTNQECNNDFNQSIESIDINDLVNEESLDEDIEFKKLINDCSSIGHGDNHGYHQAVACQTESAGKMTTIIDNNSMGLDFGFTSLSEWLPDLLKEVELVLQSCVLGDDLPKEVIPKMNKFRNLLEQMYKKQLSILLNKISTIDQELQIQRKTNQELKHKSDFIMNELQEKESIQKNVRMRLDQEMCNNTRLSEANKNQKDQNRSIKTDLSNQIFHLKEELERERNKSSDISRQLRNEQHSREQMEAVLSVTNERIKLVEQSNVDLIKKSRQQTDFQQKEMDNFNSEYAELFRRCNNYKIQIESLQQNLQYQVMECDKTKNELQETIQSNTEYRDKMNEFVKIYQERRKKLETEVNEMHQLLQISCRREITFVKETETLRVKCQSVKNMCKDYXXXXXXXXXFIGDLHTKHDEERQSWLNMINGSIDAKDKDDGILSQVNWLLKMLQLRIELLVLHYIPDLSSQGKANEWNVNAEVKSMLKCRPYSADSNINRNXXXXXXXXXXXXXXXXXXXXYLSNGQIHLPKNGSCIPESNPKISLMTERNSLNQNNMTNRPKPTGXXXXXXXXXXXXXXXXHKETSLFHLEIDSMLELQGKLDHSEKWRQLLIDQKKYILSQLSRKMCNFIVTPLLTEQQQILIHKNFFKFKFRAVGFAIIAHVRFNKTNQPTSNITLSSRLNKIGLQLPQPKFSTNIPINKFNQYSNFFSKTRLQE---XXXXXXDSQYHSFIKSSSENFSNKYSESLTTHLKNNLKFPPNETVKEPVNSNTSANNIPTHYVASNLNPIVCTSPVFDRNKKRLTKNNSSIIPKSIHKSTGSNIPVATNSNINSLRKSYPLSITKQKVHCPIERNHSDRPANFSNTLKQIDDFEKRLDKY 6597
            MNKNSKKAKPLSNKTSENNTNSVNPTETPILNSPAQIQCKTKPFSDSRIRIKKDHQIQEPSYSNKRTLKKFSTKPMSVGINQPEIMLKSYSDDLNTNIFKSLSETSVRLLTEIISNINKTNQILTSRNTEKSQTENRDYTPLVSEYLQYLMGINDMDFRNENCRQHPIYDMLQELQFNAMKITEKQAILNKSNNGLSQTLERFYQEQLNKCYDDIKRLKRQLESTQQLLTSKMPADATQVLFEAKQKLQAELESVDREYARRMSRERKRLEDSENLYNELLVDHENMQKKYISNINSLNQQIEQLERKIEADRRFIDQQQFEWELERSQHNVDMENLNRSIGRIKKSSSCSDNLSDCSESPKKEKIKKSNSLPKSKQRSFSVELTEISCNEIATQTIQNDVIILKAHLVFADLEKYDEFQDLKEEVVNSFMLLINFIHLLVDLIFDVTEKNKLNVSLKILHEFNSKQSCNIIFQLDHLQWDCMREEILNFRDFLNNFINEQANLKHNCDRIQEDLQISATDMALKQDIITSLGEEIQSLMEFQRNLSKDYDILQSMLEEKQNELDNLSAKLLSQQTIDEYEDTIQNLNKEIIEKQNIIDERDEDLYVLKEDKELMEQELKSSKEKYEAIIIQHNDIYQDLEKEMKDCQSLNHEFQDQINNYLIKIREQDEEISNINEKQISLIDQISELTEKNDILIHKNLDLNDETKLTELRLSEMKEQHKLEVLKINTSLEEIEKFNRELMEKTKIKQGIFESASCQTDRDNNESMCRDSLMSCVNIQDKLLTTIDKLKREYVREKELRQQIEIKYKHFAAQYETLSSKVILMGNKVNETRTYTNQECNNDFNQSIESIDINDLVNEESLDEDIEFKKLINDCSSIGHGDNHGYHQAVACQTESAGKMTTIIDNNSMGLDFGFTSLSEWLPDLLKEVELVLQSCVLGDDLPKEVIPKMNKFRNLLEQMYKKQLSILLNKISTIDQELQIQRKTNQELKHKSDFIMNELQEKESIQKNVRMRLDQEMCNNTRLSEANKNQKDQNRSIKTDLSNQIFHLKEELERERNKSSDISRQLRNEQHSREQMEAVLSVTNERIKLVEQSNVDLIKKSRQQTDFQQKEMDNFNSEYAELFRRCNNYKIQIESLQQNLQYQVMECDKTKNELQETIQSNTEYRDKMNEFVKIYQERRKKLETEVNEMHQLLQISCRREITFVKETETLRVKCQSVKNMCKDYEKKLKELEKFIGDLHTKHDEERQSWLNMING+IDAKDKDDGILSQVNWLLKMLQLRIELLVLHYIPDLSSQGKANEWNVNAEVKSMLKCRPYSADSNINRNSSELLMLLQSIQSMQSMINLYLSNGQIHLPKNGSCIPESNPKISLMTERNSLNQNNMTNRPKPTGSSDSLDSISDTDSASDHKETSLFHLEIDSMLELQGKLDHSEK RQLLIDQKKYILSQLSRKMCNFIVTPLLTEQQQILIHKNFFKFKFRAVGFAIIAHVRFNKTNQPTSNITLSSRLNKIGLQLPQPKFSTNIPINKFNQYSNFFSKTRLQE   NNNNNNDSQYHSFIKSSSENFSNKYSESLTTHLKNNLKFPPNETVKEPVNSNTSANNIPTHYVASNLNPIVCTSPVFDRNKKRLTKNNSSIIPKSIHKSTGSNIPVATNSNINSLRKSYPLSITKQKVH PIERNHSDRPANFSNTLKQIDDFEKRLDKY
Sbjct:    1 MNKNSKKAKPLSNKTSENNTNSVNPTETPILNSPAQIQCKTKPFSDSRIRIKKDHQIQEPSYSNKRTLKKFSTKPMSVGINQPEIMLKSYSDDLNTNIFKSLSETSVRLLTEIISNINKTNQILTSRNTEKSQTENRDYTPLVSEYLQYLMGINDMDFRNENCRQHPIYDMLQELQFNAMKITEKQAILNKSNNGLSQTLERFYQEQLNKCYDDIKRLKRQLESTQQLLTSKMPADATQVLFEAKQKLQAELESVDREYARRMSRERKRLEDSENLYNELLVDHENMQKKYISNINSLNQQIEQLERKIEADRRFIDQQQFEWELERSQHNVDMENLNRSIGRIKKSSSCSDNLSDCSESPKKEKIKKSNSLPKSKQRSFSVELTEISCNEIATQTIQNDVIILKAHLVFADLEKYDEFQDLKEEVVNSFMLLINFIHLLVDLIFDVTEKNKLNVSLKILHEFNSKQSCNIIFQLDHLQWDCMREEILNFRDFLNNFINEQANLKHNCDRIQEDLQISATDMALKQDIITSLGEEIQSLMEFQRNLSKDYDILQSMLEEKQNELDNLSAKLLSQQTIDEYEDTIQNLNKEIIEKQNIIDERDEDLYVLKEDKELMEQELKSSKEKYEAIIIQHNDIYQDLEKEMKDCQSLNHEFQDQINNYLIKIREQDEEISNINEKQISLIDQISELTEKNDILIHKNLDLNDETKLTELRLSEMKEQHKLEVLKINTSLEEIEKFNRELMEKTKIKQGIFESASCQTDRDNNESMCRDSLMSCVNIQDKLLTTIDKLKREYVREKELRQQIEIKYKHFAAQYETLSSKVILMGNKVNETRTYTNQECNNDFNQSIESIDINDLVNEESLDEDIEFKKLINDCSSIGHGDNHGYHQAVACQTESAGKMTTIIDNNSMGLDFGFTSLSEWLPDLLKEVELVLQSCVLGDDLPKEVIPKMNKFRNLLEQMYKKQLSILLNKISTIDQELQIQRKTNQELKHKSDFIMNELQEKESIQKNVRMRLDQEMCNNTRLSEANKNQKDQNRSIKTDLSNQIFHLKEELERERNKSSDISRQLRNEQHSREQMEAVLSVTNERIKLVEQSNVDLIKKSRQQTDFQQKEMDNFNSEYAELFRRCNNYKIQIESLQQNLQYQVMECDKTKNELQETIQSNTEYRDKMNEFVKIYQERRKKLETEVNEMHQLLQISCRREITFVKETETLRVKCQSVKNMCKDYEKKLKELEKFIGDLHTKHDEERQSWLNMINGNIDAKDKDDGILSQVNWLLKMLQLRIELLVLHYIPDLSSQGKANEWNVNAEVKSMLKCRPYSADSNINRNSSELLMLLQSIQSMQSMINLYLSNGQIHLPKNGSCIPESNPKISLMTERNSLNQNNMTNRPKPTGSSDSLDSISDTDSASDHKETSLFHLEIDSMLELQGKLDHSEKLRQLLIDQKKYILSQLSRKMCNFIVTPLLTEQQQILIHKNFFKFKFRAVGFAIIAHVRFNKTNQPTSNITLSSRLNKIGLQLPQPKFSTNIPINKFNQYSNFFSKTRLQENNNNNNNNNDSQYHSFIKSSSENFSNKYSESLTTHLKNNLKFPPNETVKEPVNSNTSANNIPTHYVASNLNPIVCTSPVFDRNKKRLTKNNSSIIPKSIHKSTGSNIPVATNSNINSLRKSYPLSITKQKVHSPIERNHSDRPANFSNTLKQIDDFEKRLDKY 1706          
BLAST of SMED30002155 vs. Planmine SMEST
Match: SMESG000074740.1 (SMESG000074740.1)

HSP 1 Score: 830.091 bits (2143), Expect = 0.000e+0
Identity = 434/435 (99.77%), Postives = 434/435 (99.77%), Query Frame = 1
Query:   55 MTLIKEIIKEDIMNREKLLDKVIIEWNSRYKVNLEKLSNKYKVKTKXXXXXXXXXXXXYRININDNEIAYKLIKKAIKCKAYAETEYQSIKDIRSELEDCQMIKELGTSLRKLEEEKSEQNVKIKTLTGKNLELMQEKQYLEKSLQELNLQSKLSMNGKVPIEKLAQSEYTDRKSALTAKLESYTNRAQETKRINKNPEYCHIAPNFNLFXXXXXXXXXXXXXXKDKVRDGHFTNTNKHMNIVNSPDKFESGIHQVTKSTPIKENTKSDTERNNSTHLSAINNLTQDLKEIQKYFDKKEDESMISPTHILDWEMSQISFDELKDQNANGFMLEGKEDPDDDQLATSHGPCRNSCCGDEKLNTNPLTAIPTVNNSLQFSGGKIVCDIGKLQTELREEKEKFNNLSNQIAELYALVHGSEKIETNEMPTCSGDRLSK 1359
            MTLIKEIIKEDIMNREKLLDKVIIEWNSRYKVNLEKLSNKYKVKTKSSSGSESDSESDYRININDNEIAYKLIKKAIKCKAYAETEYQSIKDIRSELEDCQMIKELGTSLRKLEEEKSEQNVKIKTLTGKNLELMQEKQYLEKSLQELNLQSKLSMNGKVPIEKLAQSEYTDRKSALTAKLESYTNRAQETKRINKNPEYCHIAPNFNLFSDSELSISEEDSLEKDKVRDGHFTNTNKHMNIVNSPDKFESGIHQVTKSTPIKENTKSDTERNNSTHLSAINNLTQDLKEIQKYFDKKEDESMISPTHILDWEMSQISFDELKDQNANGFMLEGKEDPDDDQLATSHGPCRNSCCGDEKLNTNPLTA PTVNNSLQFSGGKIVCDIGKLQTELREEKEKFNNLSNQIAELYALVHGSEKIETNEMPTCSGDRLSK
Sbjct:    1 MTLIKEIIKEDIMNREKLLDKVIIEWNSRYKVNLEKLSNKYKVKTKSSSGSESDSESDYRININDNEIAYKLIKKAIKCKAYAETEYQSIKDIRSELEDCQMIKELGTSLRKLEEEKSEQNVKIKTLTGKNLELMQEKQYLEKSLQELNLQSKLSMNGKVPIEKLAQSEYTDRKSALTAKLESYTNRAQETKRINKNPEYCHIAPNFNLFSDSELSISEEDSLEKDKVRDGHFTNTNKHMNIVNSPDKFESGIHQVTKSTPIKENTKSDTERNNSTHLSAINNLTQDLKEIQKYFDKKEDESMISPTHILDWEMSQISFDELKDQNANGFMLEGKEDPDDDQLATSHGPCRNSCCGDEKLNTNPLTAFPTVNNSLQFSGGKIVCDIGKLQTELREEKEKFNNLSNQIAELYALVHGSEKIETNEMPTCSGDRLSK 435          
BLAST of SMED30002155 vs. Planmine SMEST
Match: SMESG000074740.1 (SMESG000074740.1)

HSP 1 Score: 807.749 bits (2085), Expect = 0.000e+0
Identity = 422/423 (99.76%), Postives = 422/423 (99.76%), Query Frame = 1
Query:   91 MNREKLLDKVIIEWNSRYKVNLEKLSNKYKVKTKXXXXXXXXXXXXYRININDNEIAYKLIKKAIKCKAYAETEYQSIKDIRSELEDCQMIKELGTSLRKLEEEKSEQNVKIKTLTGKNLELMQEKQYLEKSLQELNLQSKLSMNGKVPIEKLAQSEYTDRKSALTAKLESYTNRAQETKRINKNPEYCHIAPNFNLFXXXXXXXXXXXXXXKDKVRDGHFTNTNKHMNIVNSPDKFESGIHQVTKSTPIKENTKSDTERNNSTHLSAINNLTQDLKEIQKYFDKKEDESMISPTHILDWEMSQISFDELKDQNANGFMLEGKEDPDDDQLATSHGPCRNSCCGDEKLNTNPLTAIPTVNNSLQFSGGKIVCDIGKLQTELREEKEKFNNLSNQIAELYALVHGSEKIETNEMPTCSGDRLSK 1359
            MNREKLLDKVIIEWNSRYKVNLEKLSNKYKVKTKSSSGSESDSESDYRININDNEIAYKLIKKAIKCKAYAETEYQSIKDIRSELEDCQMIKELGTSLRKLEEEKSEQNVKIKTLTGKNLELMQEKQYLEKSLQELNLQSKLSMNGKVPIEKLAQSEYTDRKSALTAKLESYTNRAQETKRINKNPEYCHIAPNFNLFSDSELSISEEDSLEKDKVRDGHFTNTNKHMNIVNSPDKFESGIHQVTKSTPIKENTKSDTERNNSTHLSAINNLTQDLKEIQKYFDKKEDESMISPTHILDWEMSQISFDELKDQNANGFMLEGKEDPDDDQLATSHGPCRNSCCGDEKLNTNPLTA PTVNNSLQFSGGKIVCDIGKLQTELREEKEKFNNLSNQIAELYALVHGSEKIETNEMPTCSGDRLSK
Sbjct:    1 MNREKLLDKVIIEWNSRYKVNLEKLSNKYKVKTKSSSGSESDSESDYRININDNEIAYKLIKKAIKCKAYAETEYQSIKDIRSELEDCQMIKELGTSLRKLEEEKSEQNVKIKTLTGKNLELMQEKQYLEKSLQELNLQSKLSMNGKVPIEKLAQSEYTDRKSALTAKLESYTNRAQETKRINKNPEYCHIAPNFNLFSDSELSISEEDSLEKDKVRDGHFTNTNKHMNIVNSPDKFESGIHQVTKSTPIKENTKSDTERNNSTHLSAINNLTQDLKEIQKYFDKKEDESMISPTHILDWEMSQISFDELKDQNANGFMLEGKEDPDDDQLATSHGPCRNSCCGDEKLNTNPLTAFPTVNNSLQFSGGKIVCDIGKLQTELREEKEKFNNLSNQIAELYALVHGSEKIETNEMPTCSGDRLSK 423          
The following BLAST results are available for this feature:
BLAST of SMED30002155 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002155 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002155 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002155 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002155 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002155 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002155 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002155 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002155 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002155 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002155 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002155 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002155 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002155 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000074739.10.000e+099.65SMESG000074739.1[more]
SMESG000074740.10.000e+099.77SMESG000074740.1[more]
SMESG000074740.10.000e+099.76SMESG000074740.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002155 ID=SMED30002155|Name=SMED30002155|organism=Schmidtea mediterranea sexual|type=transcript|length=6793bp
CGAACCAATAATTAATCAAAATTGAATTTTTGTGATTAATTTAATATTTT
TTAAATGACTTTGATAAAAGAGATAATTAAAGAAGACATTATGAATCGTG
AGAAATTATTAGATAAAGTCATTATTGAGTGGAACTCTCGTTACAAAGTT
AATTTGGAAAAACTTTCAAACAAATATAAAGTAAAAACCAAATCATCATC
TGGAAGTGAGAGCGATAGCGAAAGTGATTATAGAATTAATATAAATGATA
ATGAAATAGCTTATAAGTTAATTAAAAAAGCTATTAAATGTAAAGCATAT
GCTGAAACAGAGTATCAATCAATAAAAGACATTAGATCTGAATTAGAAGA
TTGTCAAATGATAAAAGAACTTGGAACATCATTGCGTAAATTGGAAGAAG
AGAAATCTGAACAAAATGTTAAAATAAAAACATTAACTGGGAAAAACTTG
GAATTAATGCAAGAAAAACAATACCTTGAGAAAAGTCTACAAGAACTTAA
CTTGCAATCTAAATTATCAATGAATGGAAAAGTGCCAATAGAAAAACTTG
CTCAAAGTGAGTATACTGATAGAAAATCTGCGCTAACTGCGAAATTGGAG
TCCTACACGAACAGAGCGCAAGAAACGAAGCGCATAAACAAGAATCCAGA
GTATTGTCATATTGCTCCGAATTTTAATTTATTCAGCGATAGTGAACTAT
CTATTAGCGAAGAAGATTCCCTAGAAAAGGATAAAGTCCGCGACGGACAT
TTCACCAACACAAATAAACATATGAATATTGTCAATTCCCCTGATAAATT
TGAATCAGGAATCCATCAGGTCACTAAGAGCACACCTATTAAAGAAAATA
CAAAATCTGATACTGAAAGAAATAATTCAACACATTTAAGTGCTATTAAT
AATTTAACTCAAGATTTGAAGGAAATACAAAAATATTTTGACAAAAAAGA
AGACGAATCCATGATCAGTCCAACGCATATTCTAGATTGGGAAATGAGTC
AGATATCATTTGATGAATTAAAAGATCAAAATGCAAATGGATTTATGCTA
GAGGGAAAAGAAGATCCTGATGATGATCAGTTGGCTACGAGTCACGGTCC
CTGTCGTAATTCATGTTGTGGTGATGAAAAATTGAATACAAATCCACTGA
CTGCTATTCCAACTGTGAATAATTCACTGCAGTTTTCTGGTGGTAAAATC
GTTTGTGATATTGGAAAACTACAAACGGAGTTGAGAGAGGAAAAAGAAAA
ATTCAATAATTTATCAAATCAAATAGCAGAATTGTATGCATTGGTTCACG
GATCAGAAAAAATTGAAACAAATGAAATGCCAACCTGTTCCGGAGACCGA
TTGTCAAAAAATACAGATATTCCTGGTGATCCCGTTGAACAAGTTGAAAA
AATGAAAAACTTGATAAGTACCAAATTTGGCGAAGAAAAGAATGCTAAAA
GTTCAAAATCGTTTCGAGCTCGTCTTTTTAATAAGTTTATGAATAAAAAT
TCAAAAAAAGCCAAGCCACTATCAAACAAAACCTCAGAGAATAACACAAA
TTCTGTTAATCCTACCGAAACACCAATTTTAAATAGTCCTGCGCAAATCC
AGTGTAAAACGAAACCCTTTTCCGATAGCCGAATAAGAATTAAAAAAGAT
CACCAAATACAAGAGCCATCTTATAGTAATAAACGAACATTAAAAAAATT
CTCAACAAAGCCTATGTCTGTGGGCATCAATCAACCAGAAATTATGCTCA
AATCTTACTCAGATGATTTGAATACAAATATATTCAAATCTCTATCGGAA
ACAAGCGTCAGACTGTTAACTGAGATTATTTCAAATATTAATAAAACTAA
TCAAATTCTGACCAGCAGAAACACGGAAAAATCTCAAACAGAAAATCGAG
ATTATACTCCTCTTGTGTCAGAATATTTACAATATTTAATGGGGATAAAT
GACATGGATTTTAGAAATGAAAATTGTAGACAACATCCAATATATGATAT
GCTGCAGGAGTTGCAGTTTAATGCAATGAAAATCACCGAGAAACAAGCTA
TATTAAATAAAAGTAACAATGGCCTTAGTCAAACTCTTGAAAGGTTTTAC
CAAGAGCAACTAAATAAGTGCTACGATGATATAAAAAGATTAAAAAGGCA
GCTGGAGTCAACTCAGCAATTGCTTACAAGTAAAATGCCTGCTGATGCCA
CACAAGTTCTCTTCGAAGCCAAACAAAAGTTGCAGGCTGAGTTAGAAAGC
GTTGATCGTGAATATGCTAGGCGAATGAGCAGGGAAAGGAAACGGTTGGA
GGATTCAGAAAATTTATACAATGAATTATTGGTTGATCATGAAAATATGC
AGAAGAAATATATTTCAAATATAAATTCTCTTAATCAGCAAATAGAACAA
TTGGAAAGGAAAATTGAGGCAGACAGACGATTTATTGACCAACAGCAATT
TGAATGGGAACTTGAACGTAGCCAGCACAATGTCGACATGGAAAATCTTA
ATAGATCTATTGGTCGCATAAAAAAGTCATCATCATGCAGTGACAATCTC
TCAGATTGTTCTGAATCCCCAAAGAAAGAAAAGATTAAAAAATCTAATTC
GTTACCAAAATCGAAACAACGGAGTTTTTCTGTGGAGTTAACGGAAATAT
CTTGCAACGAAATTGCTACTCAGACGATTCAAAATGACGTAATTATTCTC
AAAGCTCATTTAGTATTCGCAGATCTCGAAAAATATGATGAATTTCAAGA
CTTAAAAGAGGAAGTTGTGAACAGTTTTATGTTGTTGATCAATTTCATTC
ACCTCTTGGTCGACCTGATATTTGATGTAACGGAAAAGAATAAGCTCAAT
GTTTCACTGAAAATTCTCCATGAATTTAATTCCAAACAAAGTTGTAATAT
TATATTCCAATTGGACCACCTGCAGTGGGATTGTATGCGAGAAGAAATTT
TGAATTTTCGTGATTTCCTTAATAATTTCATTAACGAACAAGCTAATTTG
AAGCACAATTGCGATAGAATTCAAGAAGACCTTCAAATCAGTGCAACTGA
CATGGCACTTAAACAGGATATTATTACATCTCTTGGAGAAGAAATACAAA
GTTTGATGGAATTTCAAAGAAATTTATCTAAGGATTATGACATTTTACAG
TCGATGCTTGAAGAAAAACAGAACGAATTAGATAATTTATCGGCTAAATT
GCTATCACAACAAACAATAGATGAGTATGAAGATACAATTCAAAATCTCA
ATAAAGAAATAATTGAAAAACAAAACATTATTGACGAACGAGATGAAGAT
TTATACGTATTAAAGGAAGATAAAGAACTAATGGAACAAGAGTTAAAATC
ATCAAAAGAAAAATACGAAGCCATTATAATTCAACATAACGATATTTATC
AAGATCTTGAAAAAGAAATGAAAGATTGTCAAAGCTTAAATCATGAATTC
CAGGACCAAATTAATAATTATTTAATAAAAATTAGAGAACAAGATGAAGA
AATTTCCAACATTAACGAGAAGCAGATTTCTTTAATTGATCAAATATCTG
AATTGACTGAAAAAAATGACATTTTAATTCACAAAAATCTTGATTTAAAT
GATGAAACTAAACTTACAGAATTGCGTTTATCAGAAATGAAAGAACAACA
TAAGCTTGAAGTATTAAAAATAAATACTAGTTTGGAAGAAATTGAAAAAT
TTAATCGAGAATTAATGGAAAAGACTAAAATTAAGCAGGGGATTTTTGAA
TCTGCCAGCTGCCAAACTGATCGGGATAATAATGAGAGCATGTGCCGAGA
TTCCCTTATGAGTTGCGTGAATATCCAGGATAAACTATTGACAACGATTG
ATAAATTAAAACGGGAATATGTTCGCGAAAAGGAGCTCAGACAACAAATT
GAAATCAAATACAAGCATTTCGCTGCTCAATACGAAACATTATCGAGTAA
AGTTATTCTTATGGGAAATAAGGTTAACGAAACCAGGACATATACCAATC
AGGAGTGTAATAATGATTTTAACCAATCAATCGAATCTATTGATATCAAT
GATTTAGTAAACGAGGAGTCTCTAGACGAAGATATTGAATTTAAAAAACT
AATAAATGATTGTAGCTCAATCGGGCACGGCGACAATCACGGATATCATC
AAGCAGTTGCTTGTCAAACAGAATCAGCGGGGAAAATGACAACAATTATA
GACAACAATAGCATGGGTTTGGATTTCGGTTTCACTAGTCTGTCAGAGTG
GCTGCCTGATTTATTGAAGGAAGTCGAATTAGTTTTGCAGTCATGTGTTC
TCGGAGATGATCTACCAAAAGAAGTTATTCCAAAAATGAATAAATTTAGA
AATTTATTGGAACAAATGTATAAAAAGCAGCTTTCAATTTTATTGAATAA
AATCTCAACCATCGATCAAGAATTACAAATCCAAAGGAAAACAAATCAGG
AATTGAAGCATAAATCTGATTTTATTATGAATGAACTACAAGAAAAAGAA
TCTATCCAAAAGAATGTTCGAATGAGGTTAGATCAAGAAATGTGCAACAA
TACTCGCCTATCTGAAGCCAATAAGAATCAAAAAGATCAAAACAGATCGA
TTAAAACTGATCTGTCGAATCAAATATTTCATTTGAAAGAAGAATTGGAA
AGGGAAAGAAATAAATCTTCTGATATTAGTCGACAACTGAGAAATGAGCA
GCATAGCCGGGAACAGATGGAAGCCGTCTTGTCGGTTACGAATGAGAGGA
TTAAATTAGTTGAACAATCAAATGTAGATTTAATAAAGAAATCTCGTCAA
CAAACTGATTTTCAACAAAAGGAAATGGATAATTTCAATTCAGAGTACGC
GGAGCTTTTTAGGAGATGCAATAACTACAAAATCCAGATTGAATCCTTGC
AACAGAATCTACAATATCAAGTTATGGAATGTGATAAAACTAAAAATGAA
CTTCAAGAGACAATTCAGTCAAATACTGAATATCGAGACAAAATGAATGA
ATTCGTAAAAATCTATCAAGAAAGGAGAAAAAAATTGGAAACAGAAGTGA
ATGAGATGCATCAATTGTTGCAAATATCTTGTCGACGGGAAATTACGTTT
GTTAAAGAAACTGAAACTTTAAGAGTCAAATGCCAGTCAGTTAAGAACAT
GTGTAAAGATTATGAAAAAAAGCTCAAAGAACTGGAAAAATTTATTGGTG
ATTTACATACAAAGCACGATGAAGAGCGCCAGAGTTGGCTTAATATGATT
AACGGTAGCATTGATGCAAAGGATAAAGACGATGGGATTTTATCTCAAGT
AAATTGGTTGTTAAAAATGTTACAATTGCGAATAGAATTATTGGTATTGC
ATTATATACCAGATCTATCATCTCAGGGTAAGGCCAATGAATGGAATGTC
AATGCAGAGGTCAAATCAATGTTAAAGTGCCGTCCATATAGTGCTGATTC
CAACATCAATCGTAATAGTTCAGAATTGCTTATGTTGCTTCAGTCCATTC
AATCAATGCAGTCTATGATCAATCTTTATCTTAGCAATGGTCAGATTCAT
CTACCTAAAAATGGGTCTTGTATTCCCGAATCAAACCCTAAAATTTCGCT
GATGACTGAAAGAAATTCTTTGAATCAAAATAATATGACTAACAGACCGA
AACCGACCGGGTCATCGGATTCATTAGATTCTATTTCAGACACAGATTCA
GCATCTGATCACAAAGAAACGTCTCTTTTCCATCTTGAAATCGATTCGAT
GCTTGAACTTCAAGGCAAATTGGATCATTCGGAAAAATGGCGTCAGTTGC
TAATTGATCAAAAAAAATATATTTTATCCCAATTATCGCGGAAGATGTGC
AATTTTATCGTAACTCCGCTTCTCACAGAACAACAGCAGATTTTAATTCA
CAAGAATTTCTTCAAATTTAAGTTTAGAGCAGTCGGTTTCGCTATAATTG
CCCATGTCAGGTTTAATAAAACGAATCAGCCTACCTCAAATATTACTCTT
AGTAGTAGACTGAATAAAATCGGATTACAGTTACCGCAGCCAAAATTTTC
TACTAATATTCCAATAAATAAGTTTAATCAGTATTCAAATTTTTTTTCCA
AAACTCGGCTGCAAGAAAATAATAATAATAATAATGATTCTCAATACCAT
TCTTTCATCAAATCCAGTAGTGAAAATTTTAGCAATAAATACTCAGAATC
TTTGACAACACATTTGAAAAACAATCTCAAATTTCCTCCCAATGAAACTG
TGAAAGAGCCTGTCAATTCTAATACGAGTGCTAATAACATTCCAACCCAT
TATGTTGCATCTAATTTAAACCCTATTGTTTGTACTTCACCGGTGTTCGA
TAGAAATAAAAAAAGGTTGACGAAAAATAATTCATCCATTATTCCTAAGT
CTATCCATAAATCGACTGGTTCAAATATCCCTGTTGCAACTAATTCCAAT
ATTAATTCCTTGCGGAAGTCCTATCCGTTATCAATCACCAAGCAAAAAGT
GCACTGCCCTATTGAACGGAATCATTCTGACAGACCAGCAAATTTTAGTA
ACACATTAAAACAAATAGACGACTTTGAAAAGCGATTGGATAAATATTCT
GATTCCTCAAACGGGATTTGATTTATTTCAAGTATTCTCTAAATTTTTTG
CTCTTCAATTTTTAATTCTTTGCATTTATTTTTTTTATCCTACCGACATG
ATTAATTATTGATCTAATGATTAGCATTTTAATTTTGTTTGAATACATGA
CATATTTTCATGTGGATATTTATATATTATAATATAATGTATT
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protein sequence of SMED30002155-orf-1

>SMED30002155-orf-1 ID=SMED30002155-orf-1|Name=SMED30002155-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=2189bp
MTLIKEIIKEDIMNREKLLDKVIIEWNSRYKVNLEKLSNKYKVKTKSSSG
SESDSESDYRININDNEIAYKLIKKAIKCKAYAETEYQSIKDIRSELEDC
QMIKELGTSLRKLEEEKSEQNVKIKTLTGKNLELMQEKQYLEKSLQELNL
QSKLSMNGKVPIEKLAQSEYTDRKSALTAKLESYTNRAQETKRINKNPEY
CHIAPNFNLFSDSELSISEEDSLEKDKVRDGHFTNTNKHMNIVNSPDKFE
SGIHQVTKSTPIKENTKSDTERNNSTHLSAINNLTQDLKEIQKYFDKKED
ESMISPTHILDWEMSQISFDELKDQNANGFMLEGKEDPDDDQLATSHGPC
RNSCCGDEKLNTNPLTAIPTVNNSLQFSGGKIVCDIGKLQTELREEKEKF
NNLSNQIAELYALVHGSEKIETNEMPTCSGDRLSKNTDIPGDPVEQVEKM
KNLISTKFGEEKNAKSSKSFRARLFNKFMNKNSKKAKPLSNKTSENNTNS
VNPTETPILNSPAQIQCKTKPFSDSRIRIKKDHQIQEPSYSNKRTLKKFS
TKPMSVGINQPEIMLKSYSDDLNTNIFKSLSETSVRLLTEIISNINKTNQ
ILTSRNTEKSQTENRDYTPLVSEYLQYLMGINDMDFRNENCRQHPIYDML
QELQFNAMKITEKQAILNKSNNGLSQTLERFYQEQLNKCYDDIKRLKRQL
ESTQQLLTSKMPADATQVLFEAKQKLQAELESVDREYARRMSRERKRLED
SENLYNELLVDHENMQKKYISNINSLNQQIEQLERKIEADRRFIDQQQFE
WELERSQHNVDMENLNRSIGRIKKSSSCSDNLSDCSESPKKEKIKKSNSL
PKSKQRSFSVELTEISCNEIATQTIQNDVIILKAHLVFADLEKYDEFQDL
KEEVVNSFMLLINFIHLLVDLIFDVTEKNKLNVSLKILHEFNSKQSCNII
FQLDHLQWDCMREEILNFRDFLNNFINEQANLKHNCDRIQEDLQISATDM
ALKQDIITSLGEEIQSLMEFQRNLSKDYDILQSMLEEKQNELDNLSAKLL
SQQTIDEYEDTIQNLNKEIIEKQNIIDERDEDLYVLKEDKELMEQELKSS
KEKYEAIIIQHNDIYQDLEKEMKDCQSLNHEFQDQINNYLIKIREQDEEI
SNINEKQISLIDQISELTEKNDILIHKNLDLNDETKLTELRLSEMKEQHK
LEVLKINTSLEEIEKFNRELMEKTKIKQGIFESASCQTDRDNNESMCRDS
LMSCVNIQDKLLTTIDKLKREYVREKELRQQIEIKYKHFAAQYETLSSKV
ILMGNKVNETRTYTNQECNNDFNQSIESIDINDLVNEESLDEDIEFKKLI
NDCSSIGHGDNHGYHQAVACQTESAGKMTTIIDNNSMGLDFGFTSLSEWL
PDLLKEVELVLQSCVLGDDLPKEVIPKMNKFRNLLEQMYKKQLSILLNKI
STIDQELQIQRKTNQELKHKSDFIMNELQEKESIQKNVRMRLDQEMCNNT
RLSEANKNQKDQNRSIKTDLSNQIFHLKEELERERNKSSDISRQLRNEQH
SREQMEAVLSVTNERIKLVEQSNVDLIKKSRQQTDFQQKEMDNFNSEYAE
LFRRCNNYKIQIESLQQNLQYQVMECDKTKNELQETIQSNTEYRDKMNEF
VKIYQERRKKLETEVNEMHQLLQISCRREITFVKETETLRVKCQSVKNMC
KDYEKKLKELEKFIGDLHTKHDEERQSWLNMINGSIDAKDKDDGILSQVN
WLLKMLQLRIELLVLHYIPDLSSQGKANEWNVNAEVKSMLKCRPYSADSN
INRNSSELLMLLQSIQSMQSMINLYLSNGQIHLPKNGSCIPESNPKISLM
TERNSLNQNNMTNRPKPTGSSDSLDSISDTDSASDHKETSLFHLEIDSML
ELQGKLDHSEKWRQLLIDQKKYILSQLSRKMCNFIVTPLLTEQQQILIHK
NFFKFKFRAVGFAIIAHVRFNKTNQPTSNITLSSRLNKIGLQLPQPKFST
NIPINKFNQYSNFFSKTRLQENNNNNNDSQYHSFIKSSSENFSNKYSESL
TTHLKNNLKFPPNETVKEPVNSNTSANNIPTHYVASNLNPIVCTSPVFDR
NKKRLTKNNSSIIPKSIHKSTGSNIPVATNSNINSLRKSYPLSITKQKVH
CPIERNHSDRPANFSNTLKQIDDFEKRLDKYSDSSNGI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0003116parenchymal cell
Vocabulary: molecular function
TermDefinition
GO:0003779actin binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005635nuclear envelope
GO:0005737cytoplasm
GO:0005783endoplasmic reticulum
GO:0005789endoplasmic reticulum membrane
GO:0005794Golgi apparatus
GO:0005815microtubule organizing center
GO:0005856cytoskeleton
GO:0016020membrane
GO:0016021integral component of membrane
GO:0031965nuclear membrane
GO:0032580Golgi cisterna membrane
GO:0042175nuclear outer membrane-endoplasmic reticulum membrane network
Vocabulary: biological process
TermDefinition
GO:0007275multicellular organism development
GO:0009847spore germination
GO:0016337single organismal cell-cell adhesion
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 1244..1264
NoneNo IPR availableCOILSCoilCoilcoord: 816..836
NoneNo IPR availableCOILSCoilCoilcoord: 572..707
NoneNo IPR availableCOILSCoilCoilcoord: 1068..1099
NoneNo IPR availableCOILSCoilCoilcoord: 230..257
NoneNo IPR availableCOILSCoilCoilcoord: 274..344
NoneNo IPR availableCOILSCoilCoilcoord: 715..735
NoneNo IPR availableCOILSCoilCoilcoord: 1142..1222
NoneNo IPR availableCOILSCoilCoilcoord: 523..543
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 381..401
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1408..1429
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1408..1431
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 34..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 386..400