Vacuole membrane protein 1

Overview
NameVacuole membrane protein 1
Smed IDSMED30002139
Length (bp)1317
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Vacuole membrane protein 1 (SMED30002139) t-SNE clustered cells

Violin plots show distribution of expression levels for Vacuole membrane protein 1 (SMED30002139) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Vacuole membrane protein 1 (SMED30002139) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Vacuole membrane protein 1 (SMED30002139) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002139

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30002139h1SMcG0001902 dd_Smed_v4_1156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30002139h1SMcG0001902 dd_Smed_v4_1156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30002139h1SMcG0001902 dd_Smed_v4_1156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30002139h1SMcG0001902 dd_Smed_v4_1156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30002139h1SMcG0001902 dd_Smed_v4_1156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30002139h1SMcG0001902 dd_Smed_v4_1156_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Human
Match: VMP1 (vacuole membrane protein 1 [Source:HGNC Symbol;Acc:HGNC:29559])

HSP 1 Score: 279.641 bits (714), Expect = 9.785e-90
Identity = 159/352 (45.17%), Postives = 231/352 (65.62%), Query Frame = 2 
Query:  179 LHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTL-IVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSV-ETEQPQD--------ESA--WHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIH---KAIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQ 1189
            L R PL+T+ Y  +E + ++ ++T  L   + I+    +    L +   VEG HQ+ +    K+F  + +WI LG LSS G G+GLHTF+LYLGP IA VT+AAYEC S NFPEPPYP+Q +CPD+       +L  ++ K+ +E+ +WG GTAIGELPPYFMARAA LSG+  + E+ Q+        ESA  +  RA+  ++ +V++VGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKA+ KM +Q  FV+  FS+  V  + + I  VP  G SL     E+L  QR+K+H   +    + ++WL  +F  + ++++  F++SII+S+A +YA R++Q
Sbjct:   46 LWRQPLITLQYFSLEILVILKEWTSKLWHRQSIVVSFLLLLAVLIATYYVEGVHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSVNFPEPPYPDQIICPDEEGTEGTISLWSIISKVRIEACMWGIGTAIGELPPYFMARAARLSGAEPDDEEYQEFEEMLEHAESAQDFASRAKLAVQKLVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIITFSKHIVEQMVAFIGAVPGIGPSLQKPFQEYLEAQRQKLHHKSEMGTPQGENWLSWMFEKLVVVMVCYFILSIINSMAQSYAKRIQQ 397
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Human
Match: VMP1 (vacuole membrane protein 1 [Source:HGNC Symbol;Acc:HGNC:29559])

HSP 1 Score: 168.703 bits (426), Expect = 3.167e-49
Identity = 96/216 (44.44%), Postives = 136/216 (62.96%), Query Frame = 2 
Query:  623 GFGTAIGELPPYFMARAAALSGS-VETEQPQD--------ESA--WHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFS-------------QDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIH---KAIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQ 1189
            G GTAIGELPPYFMARAA LSG+  + E+ Q+        ESA  +  RA+  ++ +V++VGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKA+ KM +Q  FV+  FS             +D  A        VP  G SL     E+L  QR+K+H   +    + ++WL  +F  + ++++  F++SII+S+A +YA R++Q
Sbjct:    1 GIGTAIGELPPYFMARAARLSGAEPDDEEYQEFEEMLEHAESAQDFASRAKLAVQKLVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIITFSKHIVEQMVAFIGLEDNGALQPPPPSAVPGIGPSLQKPFQEYLEAQRQKLHHKSEMGTPQGENWLSWMFEKLVVVMVCYFILSIINSMAQSYAKRIQQ 216
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Human
Match: VMP1 (vacuole membrane protein 1 [Source:HGNC Symbol;Acc:HGNC:29559])

HSP 1 Score: 53.9138 bits (128), Expect = 3.380e-9
Identity = 24/34 (70.59%), Postives = 27/34 (79.41%), Query Frame = 2 
Query:  617 LWGFGTAIGELPPYFMARAAALSGSVETEQPQDE 718
            +WG GTAIGELPPYFMARAA LSG+    +P DE
Sbjct:    1 MWGIGTAIGELPPYFMARAARLSGA----EPDDE 30
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Celegans
Match: epg-3 (Ectopic P granules protein 3 [Source:UniProtKB/Swiss-Prot;Acc:Q9XWU8])

HSP 1 Score: 256.529 bits (654), Expect = 1.264e-80
Identity = 156/390 (40.00%), Postives = 224/390 (57.44%), Query Frame = 2 
Query:  125 DPENGVKKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFIC-AFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVETEQPQDESAWH--------------------RRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAI---VSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWKGNYNECIDD 1222
             P      + E++ ++    R P + I Y+LME  +L ++    +L+HK ++ +  I    +++  +   GAHQ+ +    K   WW WW+ LG LSS G GSGLHTF++YLGP IA VTMAAYEC S +FP+PPYPE   CP       +    +V K+ +ES+LWG GTA+GELPPYFMARAA +SG    ++P DE                         RA+  ++  + R+GF GI+L AS+PNPLFDLAG+TCGHFLVPF +FFGAT++GKA+ KM +QM FV+  FS          ++ +P  G  +   I + L +QR+ +HK       +  S+L    S +   +IL F +SI++SLA +Y  RL + K   N+ + D
Sbjct:   41 SPSQNTLNRMERETIVFW--RRPHIVIPYALMEIAHLAVELFFKILAHKTVLLLTAISIGLAVYGYH-APGAHQEHVQTIEKHILWWSWWVLLGVLSSIGLGSGLHTFLIYLGPHIAAVTMAAYECQSLDFPQPPYPESIQCPSTKSSIAVTFWQIVAKVRVESLLWGAGTALGELPPYFMARAARISG----QEPDDEEYREFLELMNADKESDADQKLSIVERAKSWVEHNIHRLGFPGILLFASIPNPLFDLAGITCGHFLVPFWSFFGATLIGKALVKMHVQMGFVILAFSDHHAENFVKILEKIPAVGPYIRQPISDLLEKQRKALHKTPGEHSEQSTSYLAWGLSLMVTFMILFFFLSIVNSLAKDYHKRLWERKRRQNKDLID 423
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Fly
Match: Tango5 (gene:FBgn0052675 transcript:FBtr0071499)

HSP 1 Score: 236.498 bits (602), Expect = 3.869e-73
Identity = 145/373 (38.87%), Postives = 222/373 (59.52%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWW-GWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTL-IVVRKILLESMLWGFGTAIGELPPYFMARAAALSG-------------SVETEQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHK---------AIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQ 1189
            +++ E+ +L+L   R PL T  Y  +E   L+  ++  LL  + +++ L + +     I  ++G HQ +  E +++  W+  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYEC S  FP+PPYP+  +CP++     +  +  ++ K+ LE+ LWG GTA+GELPPYFMA+AA LSG             ++  ++ Q   +   R +  M+ +V+RVGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKAV KM +Q  FV+  F++  +      +  +P  G  L      FL  Q++++H+             +  + L  IF    + ++  FV+SI++SLA +Y  RL +
Sbjct:   29 RERLERGQLVL--WRRPLQTTKYCGLELFTLLRTWSTRLLQQRLLLATLIVLSIVFSVIYKIDGPHQ-LAIEFVRRNTWFFVYWLGLGVLSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSLRFPQPPYPDDIICPEEPYDKHVPNIWSIMSKVRLEAFLWGAGTALGELPPYFMAKAARLSGYDPEDAEELAEFEALNAKRHQKNLSMMDRGKLFMERVVERVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAVIKMHIQKIFVIIAFNETLIERAVDLLATLPVLGHKLQEPFKSFLKNQKQRLHRQQRGAAGATTGAGDSGNLLSRIFETFVIGMVCYFVVSIVNSLAQSYHKRLHK 398
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Fly
Match: Tango5 (gene:FBgn0052675 transcript:FBtr0071500)

HSP 1 Score: 237.654 bits (605), Expect = 1.679e-72
Identity = 145/371 (39.08%), Postives = 221/371 (59.57%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWW-GWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTL-IVVRKILLESMLWGFGTAIGELPPYFMARAAALSG-------------SVETEQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHK---------AIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRL 1183
            +++ E+ +L+L   R PL T  Y  +E   L+  ++  LL  + +++ L + +     I  ++G HQ +  E +++  W+  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYEC S  FP+PPYP+  +CP++     +  +  ++ K+ LE+ LWG GTA+GELPPYFMA+AA LSG             ++  ++ Q   +   R +  M+ +V+RVGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKAV KM +Q  FV+  F++  +      +  +P  G  L      FL  Q++++H+             +  + L  IF    + ++  FV+SI++SLA +Y  RL
Sbjct:  131 RERLERGQLVL--WRRPLQTTKYCGLELFTLLRTWSTRLLQQRLLLATLIVLSIVFSVIYKIDGPHQ-LAIEFVRRNTWFFVYWLGLGVLSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSLRFPQPPYPDDIICPEEPYDKHVPNIWSIMSKVRLEAFLWGAGTALGELPPYFMAKAARLSGYDPEDAEELAEFEALNAKRHQKNLSMMDRGKLFMERVVERVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAVIKMHIQKIFVIIAFNETLIERAVDLLATLPVLGHKLQEPFKSFLKNQKQRLHRQQRGAAGATTGAGDSGNLLSRIFETFVIGMVCYFVVSIVNSLAQSYHKRL 498
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Fly
Match: Tango5 (gene:FBgn0052675 transcript:FBtr0334457)

HSP 1 Score: 237.654 bits (605), Expect = 1.679e-72
Identity = 145/371 (39.08%), Postives = 221/371 (59.57%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWW-GWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTL-IVVRKILLESMLWGFGTAIGELPPYFMARAAALSG-------------SVETEQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHK---------AIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRL 1183
            +++ E+ +L+L   R PL T  Y  +E   L+  ++  LL  + +++ L + +     I  ++G HQ +  E +++  W+  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYEC S  FP+PPYP+  +CP++     +  +  ++ K+ LE+ LWG GTA+GELPPYFMA+AA LSG             ++  ++ Q   +   R +  M+ +V+RVGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKAV KM +Q  FV+  F++  +      +  +P  G  L      FL  Q++++H+             +  + L  IF    + ++  FV+SI++SLA +Y  RL
Sbjct:  131 RERLERGQLVL--WRRPLQTTKYCGLELFTLLRTWSTRLLQQRLLLATLIVLSIVFSVIYKIDGPHQ-LAIEFVRRNTWFFVYWLGLGVLSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSLRFPQPPYPDDIICPEEPYDKHVPNIWSIMSKVRLEAFLWGAGTALGELPPYFMAKAARLSGYDPEDAEELAEFEALNAKRHQKNLSMMDRGKLFMERVVERVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAVIKMHIQKIFVIIAFNETLIERAVDLLATLPVLGHKLQEPFKSFLKNQKQRLHRQQRGAAGATTGAGDSGNLLSRIFETFVIGMVCYFVVSIVNSLAQSYHKRL 498
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Fly
Match: Tango5 (gene:FBgn0052675 transcript:FBtr0300569)

HSP 1 Score: 237.654 bits (605), Expect = 1.679e-72
Identity = 145/371 (39.08%), Postives = 221/371 (59.57%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWW-GWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTL-IVVRKILLESMLWGFGTAIGELPPYFMARAAALSG-------------SVETEQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHK---------AIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRL 1183
            +++ E+ +L+L   R PL T  Y  +E   L+  ++  LL  + +++ L + +     I  ++G HQ +  E +++  W+  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYEC S  FP+PPYP+  +CP++     +  +  ++ K+ LE+ LWG GTA+GELPPYFMA+AA LSG             ++  ++ Q   +   R +  M+ +V+RVGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKAV KM +Q  FV+  F++  +      +  +P  G  L      FL  Q++++H+             +  + L  IF    + ++  FV+SI++SLA +Y  RL
Sbjct:  131 RERLERGQLVL--WRRPLQTTKYCGLELFTLLRTWSTRLLQQRLLLATLIVLSIVFSVIYKIDGPHQ-LAIEFVRRNTWFFVYWLGLGVLSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSLRFPQPPYPDDIICPEEPYDKHVPNIWSIMSKVRLEAFLWGAGTALGELPPYFMAKAARLSGYDPEDAEELAEFEALNAKRHQKNLSMMDRGKLFMERVVERVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAVIKMHIQKIFVIIAFNETLIERAVDLLATLPVLGHKLQEPFKSFLKNQKQRLHRQQRGAAGATTGAGDSGNLLSRIFETFVIGMVCYFVVSIVNSLAQSYHKRL 498
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Fly
Match: Tango5 (gene:FBgn0052675 transcript:FBtr0346377)

HSP 1 Score: 237.654 bits (605), Expect = 1.679e-72
Identity = 145/371 (39.08%), Postives = 221/371 (59.57%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWW-GWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTL-IVVRKILLESMLWGFGTAIGELPPYFMARAAALSG-------------SVETEQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHK---------AIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRL 1183
            +++ E+ +L+L   R PL T  Y  +E   L+  ++  LL  + +++ L + +     I  ++G HQ +  E +++  W+  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYEC S  FP+PPYP+  +CP++     +  +  ++ K+ LE+ LWG GTA+GELPPYFMA+AA LSG             ++  ++ Q   +   R +  M+ +V+RVGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKAV KM +Q  FV+  F++  +      +  +P  G  L      FL  Q++++H+             +  + L  IF    + ++  FV+SI++SLA +Y  RL
Sbjct:  131 RERLERGQLVL--WRRPLQTTKYCGLELFTLLRTWSTRLLQQRLLLATLIVLSIVFSVIYKIDGPHQ-LAIEFVRRNTWFFVYWLGLGVLSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSLRFPQPPYPDDIICPEEPYDKHVPNIWSIMSKVRLEAFLWGAGTALGELPPYFMAKAARLSGYDPEDAEELAEFEALNAKRHQKNLSMMDRGKLFMERVVERVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAVIKMHIQKIFVIIAFNETLIERAVDLLATLPVLGHKLQEPFKSFLKNQKQRLHRQQRGAAGATTGAGDSGNLLSRIFETFVIGMVCYFVVSIVNSLAQSYHKRL 498
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Zebrafish
Match: vmp1 (vacuole membrane protein 1 [Source:NCBI gene;Acc:336789])

HSP 1 Score: 297.36 bits (760), Expect = 8.257e-97
Identity = 171/387 (44.19%), Postives = 246/387 (63.57%), Query Frame = 2 
Query:  125 DPENGV--------KKKAEKKE-LILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINL-VEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDD-VMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET-----------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAI---VSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWKGNYNE 1210
            D +NG         +K+ +++E L L L + P +T+ Y  +E++  + ++T  L   + ++  L +  FSLFS+   +EGAHQ+ +    KKF W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYECGS NFPEPPYP Q +CP+D  ++  +    ++ K+ LE+ +WG GTAIGELPPYFMARAA +SG+              E  Q    +  RA+  ++ +V++VGF GI+ CAS+PNPLFDLAG+TCGHFL+PF TFFGAT++GKA+ KM +Q  FV+  FS+  V  + S I  +P  G SL     E+L  QR K+H       +  +SWL  +F  + L+++  F++SII+S+A +YA RL+Q K  Y+E
Sbjct:   19 DKQNGSSTDSSLRERKQLDREERLSLVLWKRPFITLQYFFLETVITLKEWTWKLWQRRGVV-FLTVVLFSLFSLAYSIEGAHQEYVQHLEKKFLWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSVNFPEPPYPAQIVCPEDEALQESISLWTIMSKVRLEACMWGAGTAIGELPPYFMARAARMSGADPDDEDYEEFEEMLEHSQSAQDFASRAKLAVQNMVQKVGFFGILACASIPNPLFDLAGITCGHFLIPFWTFFGATLIGKAIIKMHIQKLFVIITFSKHIVEQMVSLIGVIPGVGASLQKPFREYLEAQRTKLHNPAGDGAAAGESWLSWVFEKVVLVMVCYFILSIINSMAQSYAKRLQQKK--YSE 402
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Zebrafish
Match: vmp1 (vacuole membrane protein 1 [Source:NCBI gene;Acc:336789])

HSP 1 Score: 297.36 bits (760), Expect = 8.257e-97
Identity = 171/387 (44.19%), Postives = 246/387 (63.57%), Query Frame = 2 
Query:  125 DPENGV--------KKKAEKKE-LILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINL-VEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDD-VMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET-----------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAI---VSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWKGNYNE 1210
            D +NG         +K+ +++E L L L + P +T+ Y  +E++  + ++T  L   + ++  L +  FSLFS+   +EGAHQ+ +    KKF W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYECGS NFPEPPYP Q +CP+D  ++  +    ++ K+ LE+ +WG GTAIGELPPYFMARAA +SG+              E  Q    +  RA+  ++ +V++VGF GI+ CAS+PNPLFDLAG+TCGHFL+PF TFFGAT++GKA+ KM +Q  FV+  FS+  V  + S I  +P  G SL     E+L  QR K+H       +  +SWL  +F  + L+++  F++SII+S+A +YA RL+Q K  Y+E
Sbjct:   19 DKQNGSSTDSSLRERKQLDREERLSLVLWKRPFITLQYFFLETVITLKEWTWKLWQRRGVV-FLTVVLFSLFSLAYSIEGAHQEYVQHLEKKFLWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSVNFPEPPYPAQIVCPEDEALQESISLWTIMSKVRLEACMWGAGTAIGELPPYFMARAARMSGADPDDEDYEEFEEMLEHSQSAQDFASRAKLAVQNMVQKVGFFGILACASIPNPLFDLAGITCGHFLIPFWTFFGATLIGKAIIKMHIQKLFVIITFSKHIVEQMVSLIGVIPGVGASLQKPFREYLEAQRTKLHNPAGDGAAAGESWLSWVFEKVVLVMVCYFILSIINSMAQSYAKRLQQKK--YSE 402
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Xenopus
Match: vmp1 (vacuole membrane protein 1 [Source:NCBI gene;Acc:493262])

HSP 1 Score: 276.559 bits (706), Expect = 5.535e-89
Identity = 160/368 (43.48%), Postives = 232/368 (63.04%), Query Frame = 2 
Query:  140 VKKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTL-IVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET------------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAIVS---EKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWK 1195
            +K +  ++   + L R PL+T+ Y ++E +  +  ++  L   + ++  + +    L     +EG HQ+ +    KK  W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYEC S NFPEPPYP++ +CPD+       +L  ++ K+ LE+ +WG GTAIGELPPYFMARAA LSG VET            E  Q    +  RA+  ++ +V++VGF+GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKA+ KM +Q  FV+  FS+  V  + S I  +P  G SL     E+L  QR+K+H    S   + ++WL   F  + +I++  F++SII+S+A +YA R++Q K
Sbjct:    9 IKSRDREERQSIVLWRKPLITLQYFILEVLITLKDWSIRLWHRRMMVVSVLLLLAVLSVAYYIEGTHQQYVQYVEKKCLWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTIAAYECNSVNFPEPPYPDEIICPDEEGTEGAISLWTIISKVRLEACMWGAGTAIGELPPYFMARAARLSG-VETDDEEYAEFEEMLEHAQTAQDFATRAKLAVQNLVQKVGFLGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKLFVIITFSKHIVEQMVSLIGVIPSIGPSLQKPFQEYLEAQRKKLHHKEDSGAPQSENWLSWAFEKLVIIMVFYFILSIINSMAQSYAKRVQQRK 375
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Xenopus
Match: vmp1 (vacuole membrane protein 1 [Source:NCBI gene;Acc:493262])

HSP 1 Score: 276.559 bits (706), Expect = 1.214e-88
Identity = 160/367 (43.60%), Postives = 233/367 (63.49%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTL-IVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET------------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAIVS---EKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWK 1195
            + + E++ ++L   R PL+T+ Y ++E +  +  ++  L   + ++  + +    L     +EG HQ+ +    KK  W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYEC S NFPEPPYP++ +CPD+       +L  ++ K+ LE+ +WG GTAIGELPPYFMARAA LSG VET            E  Q    +  RA+  ++ +V++VGF+GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKA+ KM +Q  FV+  FS+  V  + S I  +P  G SL     E+L  QR+K+H    S   + ++WL   F  + +I++  F++SII+S+A +YA R++Q K
Sbjct:   32 RDREERQSIVL--WRKPLITLQYFILEVLITLKDWSIRLWHRRMMVVSVLLLLAVLSVAYYIEGTHQQYVQYVEKKCLWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTIAAYECNSVNFPEPPYPDEIICPDEEGTEGAISLWTIISKVRLEACMWGAGTAIGELPPYFMARAARLSG-VETDDEEYAEFEEMLEHAQTAQDFATRAKLAVQNLVQKVGFLGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKLFVIITFSKHIVEQMVSLIGVIPSIGPSLQKPFQEYLEAQRKKLHHKEDSGAPQSENWLSWAFEKLVIIMVFYFILSIINSMAQSYAKRVQQRK 395
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Xenopus
Match: vmp1 (vacuole membrane protein 1 [Source:NCBI gene;Acc:493262])

HSP 1 Score: 276.559 bits (706), Expect = 1.383e-88
Identity = 160/367 (43.60%), Postives = 233/367 (63.49%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTL-IVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET------------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAIVS---EKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWK 1195
            + + E++ ++L   R PL+T+ Y ++E +  +  ++  L   + ++  + +    L     +EG HQ+ +    KK  W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYEC S NFPEPPYP++ +CPD+       +L  ++ K+ LE+ +WG GTAIGELPPYFMARAA LSG VET            E  Q    +  RA+  ++ +V++VGF+GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKA+ KM +Q  FV+  FS+  V  + S I  +P  G SL     E+L  QR+K+H    S   + ++WL   F  + +I++  F++SII+S+A +YA R++Q K
Sbjct:   36 RDREERQSIVL--WRKPLITLQYFILEVLITLKDWSIRLWHRRMMVVSVLLLLAVLSVAYYIEGTHQQYVQYVEKKCLWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTIAAYECNSVNFPEPPYPDEIICPDEEGTEGAISLWTIISKVRLEACMWGAGTAIGELPPYFMARAARLSG-VETDDEEYAEFEEMLEHAQTAQDFATRAKLAVQNLVQKVGFLGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKLFVIITFSKHIVEQMVSLIGVIPSIGPSLQKPFQEYLEAQRKKLHHKEDSGAPQSENWLSWAFEKLVIIMVFYFILSIINSMAQSYAKRVQQRK 399
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Xenopus
Match: vmp1 (vacuole membrane protein 1 [Source:NCBI gene;Acc:493262])

HSP 1 Score: 263.462 bits (672), Expect = 1.396e-83
Identity = 156/367 (42.51%), Postives = 229/367 (62.40%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTL-IVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET------------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAIVS---EKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWK 1195
            + + E++ ++L   R PL+T+ Y ++E +  +  ++  L   + ++  + +    L     +EG HQ+ +    KK  W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYEC S NFPEPPYP++ +CPD+       +L  ++ K+ LE+ +W     IGELPPYFMARAA LSG VET            E  Q    +  RA+  ++ +V++VGF+GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKA+ KM +Q  FV+  FS+  V  + S I  +P  G SL     E+L  QR+K+H    S   + ++WL   F  + +I++  F++SII+S+A +YA R++Q K
Sbjct:   39 RDREERQSIVL--WRKPLITLQYFILEVLITLKDWSIRLWHRRMMVVSVLLLLAVLSVAYYIEGTHQQYVQYVEKKCLWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTIAAYECNSVNFPEPPYPDEIICPDEEGTEGAISLWTIISKVRLEACMW---VTIGELPPYFMARAARLSG-VETDDEEYAEFEEMLEHAQTAQDFATRAKLAVQNLVQKVGFLGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKLFVIITFSKHIVEQMVSLIGVIPSIGPSLQKPFQEYLEAQRKKLHHKEDSGAPQSENWLSWAFEKLVIIMVFYFILSIINSMAQSYAKRVQQRK 399
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Xenopus
Match: vmp1 (vacuole membrane protein 1 [Source:NCBI gene;Acc:493262])

HSP 1 Score: 250.751 bits (639), Expect = 6.724e-79
Identity = 144/318 (45.28%), Postives = 203/318 (63.84%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTL-IVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET------------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIH 1057
            + + E++ ++L   R PL+T+ Y ++E +  +  ++  L   + ++  + +    L     +EG HQ+ +    KK  W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYEC S NFPEPPYP++ +CPD+       +L  ++ K+ LE+ +WG GTAIGELPPYFMARAA LSG VET            E  Q    +  RA+  ++ +V++VGF+GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKA+ KM +Q  FV+  FS+  V  + S I  +P  G SL     E+L  QR+K+H
Sbjct:   39 RDREERQSIVL--WRKPLITLQYFILEVLITLKDWSIRLWHRRMMVVSVLLLLAVLSVAYYIEGTHQQYVQYVEKKCLWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTIAAYECNSVNFPEPPYPDEIICPDEEGTEGAISLWTIISKVRLEACMWGAGTAIGELPPYFMARAARLSG-VETDDEEYAEFEEMLEHAQTAQDFATRAKLAVQNLVQKVGFLGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKLFVIITFSKHIVEQMVSLIGVIPSIGPSLQKPFQEYLEAQRKKLH 353
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Mouse
Match: Vmp1 (vacuole membrane protein 1 [Source:MGI Symbol;Acc:MGI:1923159])

HSP 1 Score: 285.034 bits (728), Expect = 5.201e-92
Identity = 165/376 (43.88%), Postives = 239/376 (63.56%), Query Frame = 2 
Query:  125 DPENGVKKKAEKKE--LILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTL-IVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVETEQPQDES---------------AWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIH---KAIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQ 1189
            DP +  +KK   +E    + L R PL+T+ Y  +E++ ++ ++T  L   + I+    +   +L +   VEGAHQ+ +    K+F  + +WI LG LSS G G+GLHTF+LYLGP IA VT+AAYEC S NFPEPPYP+Q +CP++       +L  ++ K+ +E+ +WG GTAIGELPPYFMARAA LSG+    +P DE                 +  RA+  ++ +V++VGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKA+ KM +Q  FV+  FS+  V  + + I  VP  G SL     E+L  QR+K+H   +A   + ++WL  +F  + + ++  FV+SII+S+A NYA R++Q
Sbjct:   26 DPSSVHEKKRRDREERQNIVLWRQPLITLQYFSLETLVVLKEWTSKLWHRQSIVVSFLLLLAALVATYYVEGAHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSVNFPEPPYPDQIICPEEEGAEGAISLWSIISKVRIEACMWGIGTAIGELPPYFMARAARLSGA----EPDDEEYQEFEEMLEHAEAAQDFASRAKLAVQKLVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIVTFSKHIVEQMVTFIGAVPGIGPSLQKPFQEYLEAQRQKLHHRSEAGTPQGENWLSWMFEKLVVAMVCYFVLSIINSMAQNYAKRIQQ 397
[Close]
BLAST of Vacuole membrane protein 1 vs. UniProt/SwissProt
Match: sp|Q6NYY9|VMP1_DANRE (Vacuole membrane protein 1 OS=Danio rerio OX=7955 GN=vmp1 PE=2 SV=1)

HSP 1 Score: 295.819 bits (756), Expect = 2.430e-95
Identity = 171/387 (44.19%), Postives = 245/387 (63.31%), Query Frame = 2 
Query:  125 DPENGV--------KKKAEKKE-LILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINL-VEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDD-VMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET-----------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAI---VSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWKGNYNE 1210
            D +NG         +K+ +++E L L L + P +T+ Y  +E+   + ++T  L   + ++  L +  FSLFS+   +EGAHQ+ +    KKF W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYECGS NFPEPPYP Q +CP+D  ++  +    ++ K+ LE+ +WG GTAIGELPPYFMARAA +SG+              E  Q    +  RA+  ++ +V++VGF GI+ CAS+PNPLFDLAG+TCGHFL+PF TFFGAT++GKA+ KM +Q  FV+  FS+  V  + S I  +P  G SL     E+L  QR K+H       +  +SWL  +F  + L+++  F++SII+S+A +YA RL+Q K  Y+E
Sbjct:   19 DKQNGSSTDSSLRERKQLDREERLSLVLWKRPFITLQYFFLETAITLKEWTWKLWQRRGVV-FLTVVLFSLFSLAYSIEGAHQEYVQHLEKKFLWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSVNFPEPPYPAQIVCPEDEALQESISLWTIMSKVRLEACMWGAGTAIGELPPYFMARAARMSGADPDDEDYEEFEEMLEHSQSAQDFASRAKLAVQNMVQKVGFFGILACASIPNPLFDLAGITCGHFLIPFWTFFGATLIGKAIIKMHIQKLFVIITFSKHIVEQMVSLIGVIPGVGASLQKPFREYLEAQRTKLHNPAGDGAAAGESWLSWVFEKVVLVMVCYFILSIINSMAQSYAKRLQQKK--YSE 402
[Close]
BLAST of Vacuole membrane protein 1 vs. UniProt/SwissProt
Match: sp|Q91ZQ0|VMP1_RAT (Vacuole membrane protein 1 OS=Rattus norvegicus OX=10116 GN=Vmp1 PE=1 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 7.332e-93
Identity = 163/376 (43.35%), Postives = 240/376 (63.83%), Query Frame = 2 
Query:  125 DPE--NGVKKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTL-IVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVETEQPQDES---------------AWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIH---KAIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQ 1189
            DP   N  K++  ++   + L R PL+T+ Y  +E++ ++ ++T  L   + ++   F+   +L +   VEGAHQ+ +    K+F  + +WI LG LSS G G+GLHTF+LYLGP IA VT+AAYEC S NFPEPPYP+Q +CPD+       +L  ++ K+ +E+ +WG GTAIGELPPYFMARAA LSG+    +P DE                 +  RA+  ++ +V++VGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKA+ KM +Q  FV+  FS+  V  + + I  VP  G SL     E+L  QR+K+H   +A  ++ ++WL   F  + + ++  F++SII+S+A +YA R++Q
Sbjct:   26 DPSSVNEKKRRDREERQNIVLWRQPLITLQYFSLETLVVLKEWTSKLWHRQSMVVSFFLLLAALVATYYVEGAHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSVNFPEPPYPDQIICPDEEGTEGAISLWSIISKVRIEACMWGIGTAIGELPPYFMARAARLSGA----EPDDEEYQEFEEMLEHAETAQDFASRAKLAVQKLVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIVTFSKHIVEQMVAFIGAVPGIGPSLQKPFQEYLEAQRQKLHHRSEAGTAQGENWLSWTFEKLVVAMVCYFILSIINSMAQSYAKRIQQ 397
[Close]
BLAST of Vacuole membrane protein 1 vs. UniProt/SwissProt
Match: sp|Q99KU0|VMP1_MOUSE (Vacuole membrane protein 1 OS=Mus musculus OX=10090 GN=Vmp1 PE=1 SV=2)

HSP 1 Score: 285.034 bits (728), Expect = 3.638e-91
Identity = 165/376 (43.88%), Postives = 239/376 (63.56%), Query Frame = 2 
Query:  125 DPENGVKKKAEKKE--LILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTL-IVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVETEQPQDES---------------AWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIH---KAIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQ 1189
            DP +  +KK   +E    + L R PL+T+ Y  +E++ ++ ++T  L   + I+    +   +L +   VEGAHQ+ +    K+F  + +WI LG LSS G G+GLHTF+LYLGP IA VT+AAYEC S NFPEPPYP+Q +CP++       +L  ++ K+ +E+ +WG GTAIGELPPYFMARAA LSG+    +P DE                 +  RA+  ++ +V++VGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKA+ KM +Q  FV+  FS+  V  + + I  VP  G SL     E+L  QR+K+H   +A   + ++WL  +F  + + ++  FV+SII+S+A NYA R++Q
Sbjct:   26 DPSSVHEKKRRDREERQNIVLWRQPLITLQYFSLETLVVLKEWTSKLWHRQSIVVSFLLLLAALVATYYVEGAHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSVNFPEPPYPDQIICPEEEGAEGAISLWSIISKVRIEACMWGIGTAIGELPPYFMARAARLSGA----EPDDEEYQEFEEMLEHAEAAQDFASRAKLAVQKLVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIVTFSKHIVEQMVTFIGAVPGIGPSLQKPFQEYLEAQRQKLHHRSEAGTPQGENWLSWMFEKLVVAMVCYFVLSIINSMAQNYAKRIQQ 397
[Close]
BLAST of Vacuole membrane protein 1 vs. UniProt/SwissProt
Match: sp|Q0VCK9|VMP1_BOVIN (Vacuole membrane protein 1 OS=Bos taurus OX=9913 GN=VMP1 PE=2 SV=1)

HSP 1 Score: 283.108 bits (723), Expect = 2.250e-90
Identity = 162/376 (43.09%), Postives = 238/376 (63.30%), Query Frame = 2 
Query:  125 DPE--NGVKKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTL-IVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVETEQPQDES---------------AWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIH---KAIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQ 1189
            DP   N  K++  ++   + L R PL+T+ Y  +E++ ++ ++T  L   + I+    +    L +   VEGAHQ+ +    K+F  + +WI LG LSS G G+GLHTF+LYLGP IA VT+AAYEC S NFPEPPYP+Q +CPD+       +L  ++ K+ +E+ +WG GTAIGELPPYFMARAA LSG+    +P DE                 +  RA+  ++ +V++VGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKA+ KM +Q  FV+  FS+  V  + + I  VP  G SL     E+L  QR+K+H   +    + ++WL  +F  + ++++  F++SII+S+A +YA R++Q
Sbjct:   26 DPSSVNEKKRRDREERQNIVLWRQPLITLQYFSLETLVILKEWTSKLWHRQSIVVSFLLLLAVLTATYYVEGAHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSVNFPEPPYPDQIICPDEEGTEGTISLWSIISKVRIEACMWGIGTAIGELPPYFMARAARLSGA----EPDDEEYQEFEEMLEHAETAQDFASRAKLAVQNLVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKLFVIVAFSKHIVEQMVAFIGAVPGIGPSLQKPFQEYLEAQRQKLHHRSEMGTPQGENWLSWMFEKLVVVMVCYFILSIINSMAQSYAKRIQQ 397
[Close]
BLAST of Vacuole membrane protein 1 vs. UniProt/SwissProt
Match: sp|Q5R9K4|VMP1_PONAB (Vacuole membrane protein 1 OS=Pongo abelii OX=9601 GN=VMP1 PE=2 SV=1)

HSP 1 Score: 281.182 bits (718), Expect = 1.222e-89
Identity = 160/352 (45.45%), Postives = 232/352 (65.91%), Query Frame = 2 
Query:  179 LHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTL-IVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSV-ETEQPQD--------ESA--WHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIH---KAIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQ 1189
            L R PL+T+ Y  +E + ++ ++T  L   + I+    +    L +   VEGAHQ+ +    K+F  + +WI LG LSS G G+GLHTF+LYLGP IA VT+AAYEC S NFPEPPYP+Q +CPD+       +L  ++ K+ +E+ +WG GTAIGELPPYFMARAA LSG+  + E+ Q+        ESA  +  RA+  ++ +V++VGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKA+ KM +Q  FV+  FS+  V  + + I  VP  G SL     E+L  QR+K+H   +    + ++WL  +F  + ++++  F++SII+S+A +YA R++Q
Sbjct:   46 LWRQPLITLQYFSLEILVILKEWTSKLWHRQSIVVSFLLLLAVLIATYYVEGAHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSVNFPEPPYPDQIICPDEGGTEGTISLWSIISKVRIEACMWGIGTAIGELPPYFMARAARLSGAEPDDEEYQEFEEMLEHAESAQDFASRAKLAVQKLVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIITFSKHIVEQMVAFIGAVPGIGPSLQKPFQEYLEAQRQKLHHKSEMGTPQGENWLSWMFEKLVVVMVCYFILSIINSMAQSYAKRIQQ 397
[Close]
BLAST of Vacuole membrane protein 1 vs. TrEMBL
Match: A0A3B1KKM6 (Vacuole membrane protein 1 OS=Astyanax mexicanus OX=7994 PE=4 SV=1)

HSP 1 Score: 307.375 bits (786), Expect = 2.180e-97
Identity = 179/399 (44.86%), Postives = 253/399 (63.41%), Query Frame = 2 
Query:   65 DSNKHTLSNGXXXXXXXXXXDPENGVKKKAEKKE-LILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINL-VEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDD-VMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET-----------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAI---VSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWKGNYNE 1210
            DS+     +G K+       D     +K+ +++E L L L R PL+T+ YS +E++  + ++T  L   + ++  L +  FS+FS+   +EGAHQK +    KK  W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYECGS NFPEPPYP Q +CPD+ V+   +    ++ K+ LE+ +WG GTAIGELPPYFMARAA LSG+              EQ Q    +  RA+  ++ +V++VGF+GI+ CAS+PNPLFDLAG+TCGHFL+PF TFFGAT++GKAV KM +Q  FV+  FS+  V  + S I  +P  G SL     E+L  QR K+H  +       +SWL  +F  + L+++  F++SII+S+A +YA RL+Q K  Y+E
Sbjct:    7 DSDLPQRRSGPKENRNGNFTDASVRERKQQDREERLSLVLWRRPLVTLQYSFLETLITLKEWTWKLWRRRGVV-FLILVLFSVFSVAYSIEGAHQKYVQYLEKKLLWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSVNFPEPPYPTQIICPDEEVLPDSISLWTIMSKVRLEACMWGAGTAIGELPPYFMARAARLSGAEPDDEDYEEFEEMLEQAQSAQDFATRAKLAVQKMVQKVGFLGILACASIPNPLFDLAGITCGHFLIPFWTFFGATLIGKAVIKMHIQKLFVIITFSRHIVEQMVSLIGAIPGVGASLQKPFREYLEAQRTKLHNPVGDGTPAGESWLSWVFEKVVLVMVCYFILSIINSMAQSYAKRLQQRK--YSE 402
[Close]
BLAST of Vacuole membrane protein 1 vs. TrEMBL
Match: A0A3B4D6U8 (Vacuole membrane protein 1 OS=Pygocentrus nattereri OX=42514 PE=4 SV=1)

HSP 1 Score: 306.22 bits (783), Expect = 5.067e-97
Identity = 173/372 (46.51%), Postives = 244/372 (65.59%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINL-VEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDD-VMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET-----------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAIVS---EKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWKGNYNE 1210
            K++  ++ L L L R PLLT+ YS +E++  + ++TG L   +  +  L +  FS+FS+   +EGAHQK +    KK  W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYECGS NFPEPPYP Q +CP++ V++  +    ++ K+ LE+ +WG GTAIGELPPYFMARAA LSG+              EQ Q    +  RA+  ++ +V++VGF+GI+ CAS+PNPLFDLAG+TCGHFL+PF TFFGAT++GKAV KM +Q  FV+  FS+  V  + S I  +P  G SL     E+L  QR K+H  +       ++WL  +F  + L+++  F++SII+S+A +YA RL+Q K  Y+E
Sbjct:   34 KQQDREERLSLVLWRKPLLTLQYSFLETLVTLKEWTGRLWQRRGAV-FLVLVLFSVFSMAYSIEGAHQKYVQYLEKKILWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSVNFPEPPYPAQIICPEEEVVQDSVSLWTIMSKVRLEACMWGAGTAIGELPPYFMARAARLSGAGPDDEDYEEFEEMLEQAQSAQDFATRAKLAVQNLVQKVGFLGILACASIPNPLFDLAGITCGHFLIPFWTFFGATLIGKAVIKMHIQKLFVIITFSRHIVEQMVSLIGVIPGVGTSLQKPFREYLEAQRTKLHNPVADGTPAGENWLSWVFEKVVLVMVCYFILSIINSMAQSYAKRLQQRK--YSE 402
[Close]
BLAST of Vacuole membrane protein 1 vs. TrEMBL
Match: A0A1A8QPD8 (Vacuole membrane protein 1 OS=Nothobranchius rachovii OX=451742 GN=VMP1 PE=4 SV=1)

HSP 1 Score: 306.22 bits (783), Expect = 6.578e-97
Identity = 172/368 (46.74%), Postives = 243/368 (66.03%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINL-VEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDD-VMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET-----------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAIVSE--KQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWKGN 1201
            ++K + + L L L R P+ T+ Y L+E++ ++ ++T Y L H++    L +   SLFS+    EGAHQ+ +    KKF W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYECGS +FPEPPYP+Q +CPDD  ++  +    ++ K+ LE+ +WG GTAIGELPPYFMARAA  SG+              EQ +D   +  RA+  ++ +V++VGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKAV KM +Q  FV+  FS   V  + S I +VPR G SL     ++L  Q+ K+H A  S+    +WL  +F  + L+++  F+ISII+S+A +YA RL+Q + +
Sbjct:   34 RQKDKNERLSLVLWRRPVTTLQYFLLETLIMLKEWT-YELWHRRGAVFLLLVLCSLFSVTYSTEGAHQEYVQYLEKKFLWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSIDFPEPPYPDQIVCPDDGQLEGSVSLFSIMSKVRLEACMWGAGTAIGELPPYFMARAARQSGADPDDEDYEEFEEMLEQAEDAQDFVTRAKLGVQHMVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAVIKMHIQKLFVIITFSNHIVEQMVSLIGSVPRVGPSLQKPFSQYLEAQKNKLHHAGGSKTADDNWLSWVFEKVVLVMVCYFIISIINSMAQSYAKRLQQLRNS 400
[Close]
BLAST of Vacuole membrane protein 1 vs. TrEMBL
Match: A0A1A8R2Y2 (Vacuole membrane protein 1 OS=Nothobranchius pienaari OX=704102 GN=VMP1 PE=4 SV=1)

HSP 1 Score: 306.22 bits (783), Expect = 6.578e-97
Identity = 172/368 (46.74%), Postives = 243/368 (66.03%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINL-VEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDD-VMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET-----------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAIVSE--KQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWKGN 1201
            ++K + + L L L R P+ T+ Y L+E++ ++ ++T Y L H++    L +   SLFS+    EGAHQ+ +    KKF W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYECGS +FPEPPYP+Q +CPDD  ++  +    ++ K+ LE+ +WG GTAIGELPPYFMARAA  SG+              EQ +D   +  RA+  ++ +V++VGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKAV KM +Q  FV+  FS   V  + S I +VPR G SL     ++L  Q+ K+H A  S+    +WL  +F  + L+++  F+ISII+S+A +YA RL+Q + +
Sbjct:   34 RQKDKNERLSLVLWRRPVTTLQYFLLETLIMLKEWT-YELWHRRGAVFLLLVLCSLFSVTYSTEGAHQEYVQYLEKKFLWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSIDFPEPPYPDQIVCPDDGQLEGSVSLFSIMSKVRLEACMWGAGTAIGELPPYFMARAARQSGADPDDEDYEEFEEMLEQAEDAQDFVTRAKLGVQHMVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAVIKMHIQKLFVIITFSNHIVEQMVSLIGSVPRVGPSLQKPFSQYLEAQKNKLHHAGGSKTADDNWLSWVFEKVVLVMVCYFIISIINSMAQSYAKRLQQLRNS 400
[Close]
BLAST of Vacuole membrane protein 1 vs. TrEMBL
Match: A0A1A8DVB5 (Vacuole membrane protein 1 OS=Nothobranchius kadleci OX=1051664 GN=VMP1 PE=4 SV=1)

HSP 1 Score: 304.679 bits (779), Expect = 2.305e-96
Identity = 171/368 (46.47%), Postives = 243/368 (66.03%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINL-VEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDD-VMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET-----------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAIVSE--KQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWKGN 1201
            ++K + + L L L R P+ T+ Y L+E++ ++ ++T Y L H++    L +   SLFS+    EGAHQ+ +    KKF W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYECGS +FPEPPYP++ +CPDD  ++  +    ++ K+ LE+ +WG GTAIGELPPYFMARAA  SG+              EQ +D   +  RA+  ++ +V++VGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKAV KM +Q  FV+  FS   V  + S I +VPR G SL     ++L  Q+ K+H A  S+    +WL  +F  + L+++  F+ISII+S+A +YA RL+Q + +
Sbjct:   34 RQKDKDERLSLVLWRRPVTTLQYFLLETLIMLKEWT-YKLWHRRGAVFLLLVLCSLFSVTYSAEGAHQEYVQYLEKKFLWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSIDFPEPPYPDEIVCPDDGQLEGSVSLFSIMSKVRLEACMWGAGTAIGELPPYFMARAARQSGADPDDEDYEEFEEMLEQAEDAQDFVTRAKLGVQHMVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAVIKMHIQKLFVIITFSNHIVEQMVSLIGSVPRVGPSLQKPFSQYLEAQKNKLHHAGGSKTADDNWLSWVFEKVVLVMVCYFIISIINSMAQSYAKRLQQLRNS 400
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Cavefish
Match: vmp1 (vacuole membrane protein 1 [Source:NCBI gene;Acc:103034060])

HSP 1 Score: 307.375 bits (786), Expect = 8.259e-101
Identity = 179/399 (44.86%), Postives = 253/399 (63.41%), Query Frame = 2 
Query:   65 DSNKHTLSNGXXXXXXXXXXDPENGVKKKAEKKE-LILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINL-VEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDD-VMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET-----------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAI---VSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWKGNYNE 1210
            DS+     +G K+       D     +K+ +++E L L L R PL+T+ YS +E++  + ++T  L   + ++  L +  FS+FS+   +EGAHQK +    KK  W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYECGS NFPEPPYP Q +CPD+ V+   +    ++ K+ LE+ +WG GTAIGELPPYFMARAA LSG+              EQ Q    +  RA+  ++ +V++VGF+GI+ CAS+PNPLFDLAG+TCGHFL+PF TFFGAT++GKAV KM +Q  FV+  FS+  V  + S I  +P  G SL     E+L  QR K+H  +       +SWL  +F  + L+++  F++SII+S+A +YA RL+Q K  Y+E
Sbjct:    7 DSDLPQRRSGPKENRNGNFTDASVRERKQQDREERLSLVLWRRPLVTLQYSFLETLITLKEWTWKLWRRRGVV-FLILVLFSVFSVAYSIEGAHQKYVQYLEKKLLWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSVNFPEPPYPTQIICPDEEVLPDSISLWTIMSKVRLEACMWGAGTAIGELPPYFMARAARLSGAEPDDEDYEEFEEMLEQAQSAQDFATRAKLAVQKMVQKVGFLGILACASIPNPLFDLAGITCGHFLIPFWTFFGATLIGKAVIKMHIQKLFVIITFSRHIVEQMVSLIGAIPGVGASLQKPFREYLEAQRTKLHNPVGDGTPAGESWLSWVFEKVVLVMVCYFILSIINSMAQSYAKRLQQRK--YSE 402
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Cavefish
Match: vmp1 (vacuole membrane protein 1 [Source:NCBI gene;Acc:103034060])

HSP 1 Score: 273.863 bits (699), Expect = 1.791e-88
Identity = 154/372 (41.40%), Postives = 219/372 (58.87%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINL-VEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDD-VMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET-----------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAI---VSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWKGNYNE 1210
            K++  ++ L L L R PL+T+ YS +E++  + ++T  L   + ++  L +  FS+FS+   +EGAHQK                                     GP IA VT+AAYECGS NFPEPPYP Q +CPD+ V+   +    ++ K+ LE+ +WG GTAIGELPPYFMARAA LSG+              EQ Q    +  RA+  ++ +V++VGF+GI+ CAS+PNPLFDLAG+TCGHFL+PF TFFGAT++GKAV KM +Q  FV+  FS+  V  + S I  +P  G SL     E+L  QR K+H  +       +SWL  +F  + L+++  F++SII+S+A +YA RL+Q K  Y+E
Sbjct:   21 KQQDREERLSLVLWRRPLVTLQYSFLETLITLKEWTWKLWRRRGVV-FLILVLFSVFSVAYSIEGAHQK-------------------------------------GPHIASVTLAAYECGSVNFPEPPYPTQIICPDEEVLPDSISLWTIMSKVRLEACMWGAGTAIGELPPYFMARAARLSGAEPDDEDYEEFEEMLEQAQSAQDFATRAKLAVQKMVQKVGFLGILACASIPNPLFDLAGITCGHFLIPFWTFFGATLIGKAVIKMHIQKLFVIITFSRHIVEQMVSLIGAIPGVGASLQKPFREYLEAQRTKLHNPVGDGTPAGESWLSWVFEKVVLVMVCYFILSIINSMAQSYAKRLQQRK--YSE 352
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Sea Lamprey
Match: vmp1 (vacuole membrane protein 1 [Source:NCBI gene;Acc:336789])

HSP 1 Score: 268.47 bits (685), Expect = 2.842e-86
Identity = 155/373 (41.55%), Postives = 225/373 (60.32%), Query Frame = 2 
Query:  131 ENGVKKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPD-DVMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVETEQPQDESAWH----------------RRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHK---AIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQ 1189
            E   + + E+  L+L   R P  TI Y ++E      ++   L + + ++++  +   +L +    EG HQ+ +    KK  W  +W+ LG LSS G G+GLHTF+LYLGP IA VT++AYEC S +FP PPYP++ +CP+ + +   +    ++ K+ LE+ +WG GTAIGELPPYFMARAA LSGS    +P DE                     RA+  M+ +V+RVGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKA+ KM +Q  FV+  FS+  V  +   I +VP  G  L     ++L  QR K+H       S   SWL  +F  + ++++  FV+SI++S+A +YA R++Q
Sbjct:   37 EQRARDRKERDGLVL--WRRPFTTIKYFVLECGLETCKWASRLWARRGVVTLFLLMLAALTAAYYAEGEHQEFVRSTEKKILWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTLSAYECDSVDFPAPPYPDEIICPESEGLNVPISIWTIMSKVRLEACMWGAGTAIGELPPYFMARAARLSGS----EPDDEEYQEFEEILEHARDSQQDLGTRAKLAMQHLVQRVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKLFVIICFSKHIVEQIVGLIGSVPSVGPMLQKPFRDYLEAQRLKLHHKPGTASSTGDSWLSWVFEKVIIVMVCYFVLSIVNSMAQSYAKRVQQ 403
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Nematostella
Match: EDO42085 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2B7])

HSP 1 Score: 247.284 bits (630), Expect = 4.874e-78
Identity = 152/370 (41.08%), Postives = 216/370 (58.38%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCP--DDVMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSV-------------ETEQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIH----KAIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWK 1195
            +K  +++   + L R P+ T++Y ++E I L+  +   +L H+K  S++     +   +  V+GAH   I E      W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AA+EC S +FPEPPYP    CP    V  S +    ++ K+ LE+ +WG GTA+GELPPYFMARAA LSG                      +  W  +A+  +   V+RVGF GI++CASVPNPLFDLAG+TCGH LVPF TFFGAT++GKAV KM +Q +FV+  FS+  V  L   +  +P  G  +       L +Q+EK+     K  V+     L  +F  + + +I+ F++SII+S+A  YA RL + K
Sbjct:   15 RKLDQQERHKIVLWRRPITTLHYFVLEVIILLHDYKERVLKHRKTCSLITFITLTTLLVYNVDGAHHHYIDEIEALAVWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAWECVSLDFPEPPYPTDIKCPAPGHVSSSPVNMWTIMSKVRLEAFMWGAGTALGELPPYFMARAARLSGVEPDDEELDEVEELERMTSAAGQDLW-TKAKKFVHDFVERVGFFGILICASVPNPLFDLAGITCGHCLVPFWTFFGATLIGKAVIKMHIQKSFVILSFSKHYVEALLEFVGTIPAVGPYIQKPFKIALEKQKEKLRGKSGKGTVASPNI-LAWVFEKVVIAMIVYFLLSIINSMAQAYAKRLDEEK 382
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Medaka
Match: vmp1 (vacuole membrane protein 1 [Source:NCBI gene;Acc:110016252])

HSP 1 Score: 293.508 bits (750), Expect = 1.856e-95
Identity = 161/365 (44.11%), Postives = 238/365 (65.21%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDD-VMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET-----------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKA--IVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWK 1195
            ++K +++ L L L R P++T++Y ++E++  + + T  L   + I+ +L +     +     EG HQ  +    KKF W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYECGS +FPEPPYP++ +CP+D  + S +  L ++ K+ LE+ +WG GTAIGELPPYFMARAA  SG+              EQ +D   +  RA+  ++ +V++VGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKA+ KM +Q  FV+  FS+  V  + S I +VP+ G  L     ++L  QR K+H A      +++WL  +F  + L+++  F+ISI++S+A +YA RL+Q +
Sbjct:   34 RQKDKEERLSLVLWRRPVITLHYFVLETLIKLKELTFKLWQRRGIVFLLLVLCTVFYIAYSSEGTHQHYVQYLEKKFLWCVYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSIDFPEPPYPDEIVCPEDGSLASNISLLTIMSKVRLEACMWGAGTAIGELPPYFMARAARQSGADPEDEDYEEFEEMLEQAKDAQDFVTRAKLGVQHMVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKLFVIITFSKHIVEQMVSLIGSVPKVGPLLQKPFSQYLEAQRNKLHHAGGRAPAEENWLAWVFEKVVLVMVCYFIISIVNSMAQSYAKRLQQLR 398
[Close]
BLAST of Vacuole membrane protein 1 vs. Ensembl Medaka
Match: vmp1 (vacuole membrane protein 1 [Source:NCBI gene;Acc:110016252])

HSP 1 Score: 293.508 bits (750), Expect = 1.856e-95
Identity = 161/365 (44.11%), Postives = 238/365 (65.21%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDD-VMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVET-----------EQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKA--IVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWK 1195
            ++K +++ L L L R P++T++Y ++E++  + + T  L   + I+ +L +     +     EG HQ  +    KKF W  +W+ LG LSS G G+GLHTF+LYLGP IA VT+AAYECGS +FPEPPYP++ +CP+D  + S +  L ++ K+ LE+ +WG GTAIGELPPYFMARAA  SG+              EQ +D   +  RA+  ++ +V++VGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKA+ KM +Q  FV+  FS+  V  + S I +VP+ G  L     ++L  QR K+H A      +++WL  +F  + L+++  F+ISI++S+A +YA RL+Q +
Sbjct:   34 RQKDKEERLSLVLWRRPVITLHYFVLETLIKLKELTFKLWQRRGIVFLLLVLCTVFYIAYSSEGTHQHYVQYLEKKFLWCVYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSIDFPEPPYPDEIVCPEDGSLASNISLLTIMSKVRLEACMWGAGTAIGELPPYFMARAARQSGADPEDEDYEEFEEMLEQAKDAQDFVTRAKLGVQHMVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKLFVIITFSKHIVEQMVSLIGSVPKVGPLLQKPFSQYLEAQRNKLHHAGGRAPAEENWLAWVFEKVVLVMVCYFIISIVNSMAQSYAKRLQQLR 398
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BLAST of Vacuole membrane protein 1 vs. Ensembl Medaka
Match: ENSORLT00000015287.2 (pep primary_assembly:ASM223467v1:14:21786227:21849697:1 gene:ENSORLG00000012209.2 transcript:ENSORLT00000015287.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 267.314 bits (682), Expect = 1.104e-85
Identity = 163/365 (44.66%), Postives = 228/365 (62.47%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSV--------------ETEQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWK 1195
            + + E++EL L   + P+LT+ Y  ME +  +  + G L  H++    L I    L     +EG HQK +  A KK  W  +W  LG LSS G G+GLHTF+LYLGP IA VT+AAYECGS +FPEPPYP+Q +CP    +  +    ++ K+ LE+ +WG GTAIGELPPYFMARAA LSG                +TE  QD ++   +A+  ++ ++++VGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKAV KM +Q  FV+  FS+  V  + S    VP  G +L     E+L  Q+ K+H  +  E +SWL  +F  + ++++  FV SI++S+A +YA R +Q K
Sbjct:    4 RDREEREELWL--WKKPVLTLRYFFMELLVTLRGWMGRLWQHRQTALGLLILLVLLSIAYRIEGVHQKYVRFAEKKTLWCAYWAGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSVDFPEPPYPDQIVCPQQ--EGSISVWSIISKVRLEACMWGAGTAIGELPPYFMARAARLSGIDPDDEDYQKFEEMLDQTEASQDFAS---QAKAAVQRLIQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAVIKMHIQKLFVIITFSRHIVEQMVSLFGAVPVLGAALQKPFREYLEAQKAKLHH-LAGEGESWLSWLFEKVVVVMVCFFVCSIVNSMAQSYAKRRRQEK 360
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BLAST of Vacuole membrane protein 1 vs. Ensembl Medaka
Match: ENSORLT00000028121.1 (pep primary_assembly:ASM223467v1:14:21771912:21849697:1 gene:ENSORLG00000012209.2 transcript:ENSORLT00000028121.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 256.144 bits (653), Expect = 1.880e-80
Identity = 163/394 (41.37%), Postives = 229/394 (58.12%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDD--------------------------VMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSV--------------ETEQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAI---VSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWK 1195
            + + E++EL L   + P+LT+ Y  ME +  +  + G L  H++    L I    L     +EG HQK +  A KK  W  +W  LG LSS G G+GLHTF+LYLGP IA VT+AAYECGS +FPEPPYP+Q +CP                             ++  +    ++ K+ LE+ +WG GTAIGELPPYFMARAA LSG                +TE  QD ++   +A+  ++ ++++VGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKAV KM +Q  FV+  FS+  V  + S    VP  G +L     E+L  Q+ K+H      V  ++SWL  +F  + ++++  FV SI++S+A +YA R +Q K
Sbjct:   46 RDREEREELWL--WKKPVLTLRYFFMELLVTLRGWMGRLWQHRQTALGLLILLVLLSIAYRIEGVHQKYVRFAEKKTLWCAYWAGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSVDFPEPPYPDQIVCPQQEVTTTSSDAEQVGVEAAGVAGVTGSAALQGSISVWSIISKVRLEACMWGAGTAIGELPPYFMARAARLSGIDPDDEDYQKFEEMLDQTEASQDFAS---QAKAAVQRLIQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAVIKMHIQKLFVIITFSRHIVEQMVSLFGAVPVLGAALQKPFREYLEAQKAKLHHLAGEGVPTEESWLSWLFEKVVVVMVCFFVCSIVNSMAQSYAKRRRQEK 434
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BLAST of Vacuole membrane protein 1 vs. Ensembl Medaka
Match: ENSORLT00000031883.1 (pep primary_assembly:ASM223467v1:14:21771916:21848762:1 gene:ENSORLG00000012209.2 transcript:ENSORLT00000031883.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 237.654 bits (605), Expect = 3.015e-73
Identity = 147/345 (42.61%), Postives = 199/345 (57.68%), Query Frame = 2 
Query:  143 KKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDD--------------------------VMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSV--------------ETEQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIH 1057
            + + E++EL L   + P+LT+ Y  ME +  +  + G L  H++    L I    L     +EG HQK +  A KK  W  +W  LG LSS G G+GLHTF+LYLGP IA VT+AAYECGS +FPEPPYP+Q +CP                             ++  +    ++ K+ LE+ +WG GTAIGELPPYFMARAA LSG                +TE  QD   +  +A+  ++ ++++VGF GI+ CAS+PNPLFDLAG+TCGHFLVPF TFFGAT++GKAV KM +Q  FV+  FS+  V  + S    VP  G +L     E+L  Q+ K+H
Sbjct:   46 RDREEREELWL--WKKPVLTLRYFFMELLVTLRGWMGRLWQHRQTALGLLILLVLLSIAYRIEGVHQKYVRFAEKKTLWCAYWAGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSVDFPEPPYPDQIVCPQQEVTTTSSDAEQVGVEAAGVAGVTGSAALQGSISVWSIISKVRLEACMWGAGTAIGELPPYFMARAARLSGIDPDDEDYQKFEEMLDQTEASQD---FASQAKAAVQRLIQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAVIKMHIQKLFVIITFSRHIVEQMVSLFGAVPVLGAALQKPFREYLEAQKAKLH 385
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BLAST of Vacuole membrane protein 1 vs. Planmine SMEST
Match: SMESG000034612.1 (SMESG000034612.1)

HSP 1 Score: 761.14 bits (1964), Expect = 0.000e+0
Identity = 396/396 (100.00%), Postives = 396/396 (100.00%), Query Frame = 2 
Query:   53 MSSGDSNKHTLSNGXXXXXXXXXXDPENGVKKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIXXXXXXXXXXXXXXHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVETEQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWKGNYNECIDDCKLKNH 1240
            MSSGDSNKHTLSNGKKKLIPKKLLDPENGVKKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIFLGFLSSCGFGSGLHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVETEQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWKGNYNECIDDCKLKNH
Sbjct:    1 MSSGDSNKHTLSNGKKKLIPKKLLDPENGVKKKAEKKELILRLHRHPLLTIYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKIIFEALKKFQWWGWWIFLGFLSSCGFGSGLHTFVLYLGPFIAKVTMAAYECGSTNFPEPPYPEQFLCPDDVMKSEMKTLIVVRKILLESMLWGFGTAIGELPPYFMARAAALSGSVETEQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCASVPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQDKVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAIVSEKQSWLQLIFSYIQLIVILGFVISIIHSLADNYATRLKQWKGNYNECIDDCKLKNH 396
The following BLAST results are available for this feature:
BLAST of Vacuole membrane protein 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
VMP19.785e-9045.17vacuole membrane protein 1 [Source:HGNC Symbol;Acc... [more]
VMP13.167e-4944.44vacuole membrane protein 1 [Source:HGNC Symbol;Acc... [more]
VMP13.380e-970.59vacuole membrane protein 1 [Source:HGNC Symbol;Acc... [more]
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BLAST of Vacuole membrane protein 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
epg-31.264e-8040.00Ectopic P granules protein 3 [Source:UniProtKB/Sw... [more]
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BLAST of Vacuole membrane protein 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tango53.869e-7338.87gene:FBgn0052675 transcript:FBtr0071499[more]
Tango51.679e-7239.08gene:FBgn0052675 transcript:FBtr0071500[more]
Tango51.679e-7239.08gene:FBgn0052675 transcript:FBtr0334457[more]
Tango51.679e-7239.08gene:FBgn0052675 transcript:FBtr0300569[more]
Tango51.679e-7239.08gene:FBgn0052675 transcript:FBtr0346377[more]
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BLAST of Vacuole membrane protein 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
vmp18.257e-9744.19vacuole membrane protein 1 [Source:NCBI gene;Acc:3... [more]
vmp18.257e-9744.19vacuole membrane protein 1 [Source:NCBI gene;Acc:3... [more]
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BLAST of Vacuole membrane protein 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
vmp15.535e-8943.48vacuole membrane protein 1 [Source:NCBI gene;Acc:4... [more]
vmp11.214e-8843.60vacuole membrane protein 1 [Source:NCBI gene;Acc:4... [more]
vmp11.383e-8843.60vacuole membrane protein 1 [Source:NCBI gene;Acc:4... [more]
vmp11.396e-8342.51vacuole membrane protein 1 [Source:NCBI gene;Acc:4... [more]
vmp16.724e-7945.28vacuole membrane protein 1 [Source:NCBI gene;Acc:4... [more]
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BLAST of Vacuole membrane protein 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Vmp15.201e-9243.88vacuole membrane protein 1 [Source:MGI Symbol;Acc:... [more]
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BLAST of Vacuole membrane protein 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6NYY9|VMP1_DANRE2.430e-9544.19Vacuole membrane protein 1 OS=Danio rerio OX=7955 ... [more]
sp|Q91ZQ0|VMP1_RAT7.332e-9343.35Vacuole membrane protein 1 OS=Rattus norvegicus OX... [more]
sp|Q99KU0|VMP1_MOUSE3.638e-9143.88Vacuole membrane protein 1 OS=Mus musculus OX=1009... [more]
sp|Q0VCK9|VMP1_BOVIN2.250e-9043.09Vacuole membrane protein 1 OS=Bos taurus OX=9913 G... [more]
sp|Q5R9K4|VMP1_PONAB1.222e-8945.45Vacuole membrane protein 1 OS=Pongo abelii OX=9601... [more]
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BLAST of Vacuole membrane protein 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3B1KKM62.180e-9744.86Vacuole membrane protein 1 OS=Astyanax mexicanus O... [more]
A0A3B4D6U85.067e-9746.51Vacuole membrane protein 1 OS=Pygocentrus natterer... [more]
A0A1A8QPD86.578e-9746.74Vacuole membrane protein 1 OS=Nothobranchius racho... [more]
A0A1A8R2Y26.578e-9746.74Vacuole membrane protein 1 OS=Nothobranchius piena... [more]
A0A1A8DVB52.305e-9646.47Vacuole membrane protein 1 OS=Nothobranchius kadle... [more]
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BLAST of Vacuole membrane protein 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
vmp18.259e-10144.86vacuole membrane protein 1 [Source:NCBI gene;Acc:1... [more]
vmp11.791e-8841.40vacuole membrane protein 1 [Source:NCBI gene;Acc:1... [more]
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BLAST of Vacuole membrane protein 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
vmp12.842e-8641.55vacuole membrane protein 1 [Source:NCBI gene;Acc:3... [more]
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BLAST of Vacuole membrane protein 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Vacuole membrane protein 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO420854.874e-7841.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Vacuole membrane protein 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
vmp11.856e-9544.11vacuole membrane protein 1 [Source:NCBI gene;Acc:1... [more]
vmp11.856e-9544.11vacuole membrane protein 1 [Source:NCBI gene;Acc:1... [more]
ENSORLT00000015287.21.104e-8544.66pep primary_assembly:ASM223467v1:14:21786227:21849... [more]
ENSORLT00000028121.11.880e-8041.37pep primary_assembly:ASM223467v1:14:21771912:21849... [more]
ENSORLT00000031883.13.015e-7342.61pep primary_assembly:ASM223467v1:14:21771916:21848... [more]
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BLAST of Vacuole membrane protein 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000034612.10.000e+0100.00SMESG000034612.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002139 ID=SMED30002139|Name=Vacuole membrane protein 1|organism=Schmidtea mediterranea sexual|type=transcript|length=1317bp
ATTACACTCACAATCGCGTCATTTTAAAGTTTAAGTTAAACATCAATTAA
ACATGTCTTCTGGAGATTCTAATAAACACACATTATCAAATGGGAAGAAA
AAATTAATTCCAAAAAAATTACTCGATCCTGAAAATGGGGTAAAAAAGAA
AGCAGAAAAAAAAGAATTAATTTTGAGATTGCATAGACACCCTTTATTGA
CAATTTACTATTCTTTGATGGAGTCTATTTATTTAATATTGCAATTTACG
GGATATTTATTAAGTCACAAAAAGATAATCAGCATATTATTTATCTGTGC
ATTTTCTCTGTTTTCAATTAATTTAGTTGAAGGAGCACATCAAAAAATAA
TTTTTGAAGCCCTTAAAAAATTTCAGTGGTGGGGTTGGTGGATATTTTTA
GGGTTTTTGTCATCTTGTGGGTTTGGAAGTGGTCTTCATACATTTGTGCT
GTATTTGGGACCGTTTATTGCAAAAGTTACAATGGCAGCTTATGAATGCG
GATCGACGAATTTTCCTGAACCACCATATCCTGAACAATTTTTATGTCCT
GACGATGTTATGAAAAGTGAAATGAAGACATTAATAGTTGTGCGCAAAAT
TTTATTAGAATCTATGCTATGGGGTTTCGGCACTGCTATTGGAGAATTAC
CACCGTATTTTATGGCAAGAGCGGCGGCACTCAGTGGCTCAGTGGAAACA
GAACAGCCTCAAGATGAATCAGCGTGGCACAGGAGAGCTCAGGATACAAT
GAAAACTATTGTGAAACGCGTTGGATTTATTGGCATTGTTTTGTGCGCCT
CTGTACCGAATCCTCTATTTGATCTTGCTGGTATGACTTGCGGACACTTT
CTAGTCCCGTTTTCGACTTTCTTTGGGGCAACTGTACTCGGCAAGGCAGT
CAACAAGATGCTTTTGCAAATGGCATTTGTTGTTTTTCTTTTCTCACAGG
ACAAGGTCGCATGGTTGCAGTCATGCATCAAAAATGTGCCTCGCTATGGG
GAATCTCTGAATTCATTGATCGATGAATTCCTGATCCAGCAACGAGAGAA
GATCCACAAAGCTATTGTGTCAGAGAAACAGAGTTGGCTGCAATTGATAT
TCAGTTACATTCAACTGATTGTGATATTAGGATTTGTGATATCGATCATA
CATTCACTCGCAGACAATTACGCGACCAGATTAAAGCAGTGGAAAGGCAA
TTACAATGAGTGCATTGACGATTGCAAGCTGAAAAACCACTAATCCGATG
GCGCTGCTCACGTTCCATACAAATACTATGTTCTATTCTAAAATAAATCT
TATTGTTCCAAAAAAAA
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protein sequence of SMED30002139-orf-1

>SMED30002139-orf-1 ID=SMED30002139-orf-1|Name=SMED30002139-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=397bp
MSSGDSNKHTLSNGKKKLIPKKLLDPENGVKKKAEKKELILRLHRHPLLT
IYYSLMESIYLILQFTGYLLSHKKIISILFICAFSLFSINLVEGAHQKII
FEALKKFQWWGWWIFLGFLSSCGFGSGLHTFVLYLGPFIAKVTMAAYECG
STNFPEPPYPEQFLCPDDVMKSEMKTLIVVRKILLESMLWGFGTAIGELP
PYFMARAAALSGSVETEQPQDESAWHRRAQDTMKTIVKRVGFIGIVLCAS
VPNPLFDLAGMTCGHFLVPFSTFFGATVLGKAVNKMLLQMAFVVFLFSQD
KVAWLQSCIKNVPRYGESLNSLIDEFLIQQREKIHKAIVSEKQSWLQLIF
SYIQLIVILGFVISIIHSLADNYATRLKQWKGNYNECIDDCKLKNH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000099neuron
PLANA:0002032epidermal cell
Vocabulary: cellular component
TermDefinition
GO:0000407pre-autophagosomal structure
GO:0005773vacuole
GO:0005774vacuolar membrane
GO:0005783endoplasmic reticulum
GO:0005886plasma membrane
GO:0016020membrane
GO:0016021integral component of membrane
GO:0033116endoplasmic reticulum-Golgi intermediate compartment membrane
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
Vocabulary: biological process
TermDefinition
GO:0006887exocytosis
GO:0007029endoplasmic reticulum organization
GO:0007030Golgi organization
GO:0010506regulation of autophagy
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR10281:SF1VACUOLE MEMBRANE PROTEIN 1coord: 31..382
NoneNo IPR availablePANTHERPTHR10281MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATEDcoord: 31..382
NoneNo IPR availableTMHMMTMhelixcoord: 75..97
NoneNo IPR availableTMHMMTMhelixcoord: 258..280
NoneNo IPR availableTMHMMTMhelixcoord: 112..134
NoneNo IPR availableTMHMMTMhelixcoord: 48..70
NoneNo IPR availableTMHMMTMhelixcoord: 348..370
NoneNo IPR availableTMHMMTMhelixcoord: 236..253