SMED30002136

Overview
NameSMED30002136
Smed IDSMED30002136
Length (bp)2292
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30002136 (SMED30002136) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30002136 (SMED30002136) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30002136 (SMED30002136) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30002136 (SMED30002136) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30002136

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30002136SMESG000081604.1 Contig4254GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30002136SMESG000078281.1 Contig4254GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
nervous systemSMED30002136SMESG000078281.1 dd_Smed_v4_8853_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30002136SMESG000078281.1 dd_Smed_v4_8853_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30002136SMESG000078281.1 dd_Smed_v4_8853_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30002136SMESG000078281.1 dd_Smed_v4_8853_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002136SMESG000078281.1 Contig48971newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002136SMESG000078281.1 Contig48971uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30002136 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000365:12487..31164 -11094
Homology
BLAST of SMED30002136 vs. Planmine SMEST
Match: SMESG000078281.1 (SMESG000078281.1)

HSP 1 Score: 1321.61 bits (3419), Expect = 0.000e+0
Identity = 706/711 (99.30%), Postives = 709/711 (99.72%), Query Frame = 3
Query:   30 MVESVLCFSDSKAIKAGSICIFNQNKLFLVDNEIPSQEVLFIPNSFGILTTEEYNILKSVEPHKRIKKLQHYLNCQNLKPNDKILIMDSNLEWTVMNKNLVDDQITYSISASNKEIKEIEPAVVKRILXXXXXXXXXXXLERSNSLSKRRKEEQLKFINEIYLSNSTNLKQYSIISTEHLFSDNFLVQVLNIEVYGKIKFVISNFQTVLACISINDGIDKELENIRDQIEFQTNIGSEHFHGIIVLFDCVKEFSESPFYITNDCPNLCKSSFKYLEENDKSKFNAHDLVKFYGNKKRHYANVVKTCLINNEPCLKVSWIFPEEAQRPDLQFVKECDMVKVDDQELEFKYFEGVIITPYPILTIETTNSEYIKINDNVYFILRDSLKKIKGNVVWIGKINGMFVGILNLKRDYELKIGGKFRIKEHRMKYFKEFQYSNLLYIVPFCHLKREKYKELQEIIIEFLRSNDDCLGVVINKAMEMREKSVLPLLIENMEVTVKXXXXXXXXXXXXXRNQFETMQILLNKSIGNESIXXXXXXXXXXXXXXXXMNIFESLVQKCTNKNIKFDNGETFLTNIFKENMSNDVIIHLLDMNIFDLNQSNDGNETPLILACDKLMIDVVEKIMSYSNLNTFQIVYAQWKVLEKNKIDNPIEEKDKIINMVKMLSSKSFNEKFLSSICSICKEQTTTNVREFESPICENCLEHKSQSEIKNA 2162
            MVESVLCFSDSKAIKAGSICIFNQNKLFLVDNEIPSQEVLFIPNSFGILTTEEYNILKSVEPHKRIKKLQHYLNCQNLKPNDKILIMDSNLEWTVMNKNLVDDQITYSISASNKEIKEIEPAVVKRILSNTNTKSSNSTLERSN+LSKRRKEEQLKFINEIYLSNSTNLKQYSIISTEHLFSDNFLVQVLNIEVYGKIKFVISNFQTVLACIS+NDGIDKELENIRDQIEFQTNIGSEHFHGIIVLFDCVKEFSESPFYITNDCPNLCKSSFKYLEENDKSKFNAHDLVKFYGNKKRHYANVVKTCLINNEPCLKVSWIFPEEAQRPDLQFVKECDMVKV DQELEFKYFEGVIITPYPILTIETTNSEYIKINDNVYFILRDSLKKIKGNVVWIGKINGMFVGILNLKRDYELKIGGKFRIKEHRMKYFKEFQYSNLLYIVPFCHLKREKYKELQEIIIEFLRSNDDCLGVVINKAMEMREKSVLPLLIENMEVTVKLELYQELLLLLFERNQFETMQILLNKSIGNESIDLLLLDIGLKCLKLDKM+IFESLVQKCTNKNIKFDNGETFLTNIFKENMSNDVIIHLLDMNIFDLNQSNDGNETPLILACDKLMIDVVEKIMSYSNLNT QIVYAQWKVLEKNKIDNPIEEKDKIINMVKMLSSKSFNEKFLSSICSICKEQTTTNVREFESPICENCLEHKSQSEIKNA
Sbjct:    1 MVESVLCFSDSKAIKAGSICIFNQNKLFLVDNEIPSQEVLFIPNSFGILTTEEYNILKSVEPHKRIKKLQHYLNCQNLKPNDKILIMDSNLEWTVMNKNLVDDQITYSISASNKEIKEIEPAVVKRILSNTNTKSSNSTLERSNTLSKRRKEEQLKFINEIYLSNSTNLKQYSIISTEHLFSDNFLVQVLNIEVYGKIKFVISNFQTVLACISMNDGIDKELENIRDQIEFQTNIGSEHFHGIIVLFDCVKEFSESPFYITNDCPNLCKSSFKYLEENDKSKFNAHDLVKFYGNKKRHYANVVKTCLINNEPCLKVSWIFPEEAQRPDLQFVKECDMVKV-DQELEFKYFEGVIITPYPILTIETTNSEYIKINDNVYFILRDSLKKIKGNVVWIGKINGMFVGILNLKRDYELKIGGKFRIKEHRMKYFKEFQYSNLLYIVPFCHLKREKYKELQEIIIEFLRSNDDCLGVVINKAMEMREKSVLPLLIENMEVTVKLELYQELLLLLFERNQFETMQILLNKSIGNESIDLLLLDIGLKCLKLDKMDIFESLVQKCTNKNIKFDNGETFLTNIFKENMSNDVIIHLLDMNIFDLNQSNDGNETPLILACDKLMIDVVEKIMSYSNLNTSQIVYAQWKVLEKNKIDNPIEEKDKIINMVKMLSSKSFNEKFLSSICSICKEQTTTNVREFESPICENCLEHKSQSEIKNA 710          
BLAST of SMED30002136 vs. Planmine SMEST
Match: SMESG000007100.1 (SMESG000007100.1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.632e-10
Identity = 46/157 (29.30%), Postives = 83/157 (52.87%), Query Frame = 3
Query: 1671 MNIFESLVQKCTNKNIKFDNGETFLTNIFKENMSNDVIIHLLDMNIFDLNQSNDGNETPLILACDKLMIDVVEKIMSYS-NLNTFQIVYAQWKVLEKNK--IDNPIEEKDKIINMVKMLS-SKSFNEKFLSSICSICKEQTTTNVREFESPICENCL 2129
            + +F+ ++  CT+KN    NGETF+T + K +++   ++ +L+  +FD+N++N   + PLILAC+ L+  VV  ++  + +L   +    +  VL K+K  + N   +  K   ++++LS S  F E  L  IC    + T  N       IC + L
Sbjct:  332 LELFDEIISICTDKNAILHNGETFVTALLKSDINPATMVSILNRKLFDINKANLHKQVPLILACENLLFVVVNALLKLNCDLKYHKSQAIESVVLAKSKAELQNDHVKAKKGSEIIELLSESVDFKELHLCQICMDRNKDTALNCGHTLCSICSDKL 488          
BLAST of SMED30002136 vs. Planmine SMEST
Match: SMESG000007115.1 (SMESG000007115.1)

HSP 1 Score: 63.5438 bits (153), Expect = 4.236e-10
Identity = 31/66 (46.97%), Postives = 44/66 (66.67%), Query Frame = 3
Query: 1692 VQKCTNKNIKFDNGETFLTNIFKENMSNDVIIHLLDMNIFDLNQSNDGNETPLILACDKLMIDVVE 1889
            ++KCTN+N  F+NGETFLT   KE     +II L+  N FD+NQ N  N+T L LA D  ++++V+
Sbjct:  119 IEKCTNRNFIFNNGETFLTASIKEKYDESIIIQLIKRNFFDINQENGQNQTVLSLAKDSSLLNLVD 184          
BLAST of SMED30002136 vs. Planmine SMEST
Match: SMESG000007087.1 (SMESG000007087.1)

HSP 1 Score: 63.5438 bits (153), Expect = 6.470e-10
Identity = 49/153 (32.03%), Postives = 87/153 (56.86%), Query Frame = 3
Query: 1677 IFESLVQKCTNKNIKFDNGETFLTNIFKENMSNDVIIHLLDMNIFDLNQSNDGNETPLILACDKLMIDVVEKIMSYS-NLNTFQIVYAQWKVLEKNKIDNPIE--EKDKIINMVKMLSSKSFNEKFLSSICSICKEQTTTNVREFESPICENC 2126
            +F+ ++  C +KNI F  GETF+T + K  + +  ++ +L   +FD+N++N GNETPL+LAC+ L++DVV+ +++ + +L  ++    +  VL K K  N     + D    ++K+LS    N+   S  C+IC +++   V       C NC
Sbjct: 1594 LFDKIMSLCLDKNIIFCGGETFVTALIKSGIHSSTMVSILARKLFDINKTNLGNETPLVLACEGLLLDVVKVLVALNCDLKRYRHEAIESAVLAKLKAKNENNHLKVDLGSQIIKLLSETIDND---SIQCNICMDRSIDTVLNCGHTFCLNC 1743          

HSP 2 Score: 51.2174 bits (121), Expect = 3.236e-6
Identity = 58/252 (23.02%), Postives = 106/252 (42.06%), Query Frame = 3
Query:  492 LKFINEIYLSNSTNLKQYSIISTEHLFSDNFLVQVLNIEVYGKIKFVISNFQTVLACISINDGIDKELENIRDQIE---FQTNI----GSEHFHGIIVLFDCVKEFSESPFYITNDCPNLCKSSFKYLEENDKSKFNAHDLVKFYGNKKRHYANVVKTCLINNEPCLKVSWIFPEEAQRPDLQFVKECDMVKVDDQELEFKYFEGVIITPYPILTIETTNSEYIKINDNVYFILRDSLKKIKGNVVWIGKIN 1226
            +  I +I++  +        + T+ ++     V++L  +++G+I+     ++ +   I +           +DQI+   F   I    G+  +  +I+L D + + SE+  +  N+   + K   +Y        F   DL+K        Y  V    +IN   CL V      EA+    +++ E + +KVD+ EL+ KYF+GV I P  I + E        I + V F+  D     KG +   G  N
Sbjct:  454 IDLIKDIFVEKNQKEFSRGSVRTDDIYHVGQFVKILPFDLFGQIECFHVVYEEMFVQIQLT----------KDQIKRLPFHQRIDRAAGNAIYSTVILLKDSIAKCSENLEFF-NESSLINKECIEYRIPRPMETFRTGDLIKAGKTGNVKYFIVNNRFIINGISCLSVMESILGEAE----EYLPESEAIKVDNSELKMKYFQGVFIDPKSIRSFEKCEINDFCIGNRVEFMSMDRKSSYKGRIKSTGIAN 690          
BLAST of SMED30002136 vs. Planmine SMEST
Match: SMESG000074227.1 (SMESG000074227.1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.050e-9
Identity = 48/157 (30.57%), Postives = 80/157 (50.96%), Query Frame = 3
Query: 1677 IFESLVQKCTNKNIKFDNGETFLTNIFKENMSNDVIIHLLDMNIFDLNQSNDGNETPLILACDKLMIDVVEKIMSYS-NLNTFQIVYAQWKVLEKNKIDN-----PIEEKDKIINMVKMLSSKSFNEKFLSSICSICKEQTTTNVREFESPICENCL 2129
            +F+ ++  C  KNI    GET +T + K  + +  ++ +L+  IFD N++N  NE PLILAC  L+ DVV  +++ + +L  ++    +  VL K +  N      +E   +II   K+LS    N+   S  C IC +++   V       C NC+
Sbjct:  627 LFDKIMSLCLYKNIILCGGETVVTAVIKSGIPSSTMVSILERKIFDTNKTNLENEAPLILACKFLLFDVVNVLVALNCDLKRYRHEAIESAVLAKLEAKNENNHLKVEFGSEII---KLLSETIDND---SIQCQICMDRSKDTVLNCGHTFCLNCV 777          
The following BLAST results are available for this feature:
BLAST of SMED30002136 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002136 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002136 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002136 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002136 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002136 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002136 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002136 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002136 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002136 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002136 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002136 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002136 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002136 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000078281.10.000e+099.30SMESG000078281.1[more]
SMESG000007100.11.632e-1029.30SMESG000007100.1[more]
SMESG000007115.14.236e-1046.97SMESG000007115.1[more]
SMESG000007087.16.470e-1032.03SMESG000007087.1[more]
SMESG000074227.12.050e-930.57SMESG000074227.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002136 ID=SMED30002136|Name=SMED30002136|organism=Schmidtea mediterranea sexual|type=transcript|length=2292bp
CTATATAAATCTTATATGCAATTTATCCAATGGTAGAATCTGTCCTATGC
TTTAGTGATTCAAAAGCAATCAAGGCTGGTTCAATATGTATTTTTAATCA
AAACAAACTTTTTTTGGTTGACAATGAGATTCCTTCACAAGAAGTTCTTT
TCATTCCAAACAGCTTTGGAATTTTAACCACTGAAGAATATAATATTCTG
AAATCTGTTGAACCACATAAAAGAATAAAAAAATTACAACATTATCTCAA
TTGCCAAAATCTGAAACCAAATGACAAAATTCTAATAATGGATAGCAATT
TAGAATGGACAGTGATGAATAAAAATTTAGTCGATGATCAAATCACTTAT
TCCATTTCTGCGTCAAATAAAGAAATTAAAGAAATTGAACCAGCTGTCGT
CAAAAGAATTCTTTCTAATACAAATACGAAATCGTCAAATTCCACTCTTG
AGAGATCCAACTCTTTATCAAAAAGAAGAAAGGAAGAACAGCTCAAATTT
ATAAATGAAATATATTTGTCCAATTCCACAAATTTGAAACAATATTCAAT
TATTTCAACTGAACATTTATTTTCGGATAATTTTCTGGTTCAAGTATTAA
ATATTGAAGTATATGGCAAAATAAAGTTTGTTATTTCAAATTTCCAAACG
GTTTTAGCTTGTATTTCAATAAATGATGGCATTGATAAGGAGCTTGAAAA
TATAAGAGACCAGATAGAATTCCAAACAAATATTGGCTCGGAACATTTTC
ATGGAATCATTGTGCTATTTGATTGTGTGAAAGAGTTCTCAGAAAGTCCT
TTTTATATCACCAATGATTGTCCTAATTTATGTAAATCGAGTTTTAAATA
TCTAGAGGAAAATGATAAGAGCAAATTCAATGCTCATGATTTGGTTAAGT
TTTATGGAAATAAAAAACGTCACTATGCAAACGTTGTTAAAACTTGCTTA
ATAAATAATGAACCCTGTTTAAAGGTATCTTGGATCTTCCCAGAAGAAGC
TCAAAGACCAGATTTACAATTTGTTAAAGAGTGTGATATGGTCAAAGTTG
ATGATCAAGAATTAGAATTTAAATATTTTGAAGGAGTAATCATAACTCCA
TACCCAATTTTGACAATAGAAACTACAAATTCAGAATACATAAAAATAAA
TGATAATGTTTATTTTATTCTGAGAGATTCACTAAAAAAAATCAAAGGAA
ATGTTGTATGGATTGGTAAAATAAATGGGATGTTTGTCGGAATTTTAAAT
TTGAAAAGAGATTATGAATTGAAAATTGGTGGGAAATTTAGAATTAAAGA
ACATCGTATGAAATATTTTAAAGAATTTCAATATAGTAATTTGCTATATA
TTGTGCCCTTCTGTCATTTAAAGAGAGAGAAATATAAAGAGCTTCAAGAG
ATAATCATCGAATTCTTGCGCTCAAACGATGACTGTTTAGGCGTTGTAAT
AAACAAAGCCATGGAAATGAGAGAAAAAAGTGTTTTGCCTTTGTTGATTG
AAAATATGGAAGTGACTGTGAAACTAGAATTATATCAGGAATTGCTATTA
TTGTTATTTGAAAGAAATCAATTTGAAACGATGCAAATTTTATTAAATAA
GTCAATTGGTAATGAAAGCATTGATCTTCTTCTTTTGGATATCGGACTGA
AATGTTTAAAATTGGACAAAATGAATATATTTGAGTCACTTGTTCAAAAG
TGCACCAATAAAAACATTAAATTCGACAATGGGGAAACATTTTTAACCAA
TATTTTTAAGGAGAACATGTCTAATGATGTTATTATACATTTATTGGATA
TGAATATTTTTGATTTAAACCAATCAAATGATGGCAACGAAACGCCGTTG
ATACTTGCTTGTGACAAGCTTATGATAGATGTTGTGGAAAAAATTATGAG
CTATTCCAATTTAAATACTTTCCAAATTGTGTATGCCCAATGGAAAGTGT
TGGAAAAAAACAAAATTGACAATCCTATTGAAGAAAAAGATAAAATTATC
AATATGGTTAAAATGCTTTCATCTAAATCCTTCAATGAAAAATTTTTATC
CTCTATTTGCAGTATTTGTAAAGAACAAACTACTACAAATGTACGCGAAT
TTGAAAGTCCGATTTGTGAAAATTGTTTGGAACATAAAAGTCAATCTGAA
ATTAAAAATGCATAAAATTTTTACTTTCCAATTCAAATTGTCATTAGAGA
CGTTTTCCCAATTAATTTTTTGACAATTTATTAACATAAACTGTTTTTGA
TTATTAATTAAACATGTTAATTAACTTCATTTTAGATTTGAT
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protein sequence of SMED30002136-orf-1

>SMED30002136-orf-1 ID=SMED30002136-orf-1|Name=SMED30002136-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=712bp
MVESVLCFSDSKAIKAGSICIFNQNKLFLVDNEIPSQEVLFIPNSFGILT
TEEYNILKSVEPHKRIKKLQHYLNCQNLKPNDKILIMDSNLEWTVMNKNL
VDDQITYSISASNKEIKEIEPAVVKRILSNTNTKSSNSTLERSNSLSKRR
KEEQLKFINEIYLSNSTNLKQYSIISTEHLFSDNFLVQVLNIEVYGKIKF
VISNFQTVLACISINDGIDKELENIRDQIEFQTNIGSEHFHGIIVLFDCV
KEFSESPFYITNDCPNLCKSSFKYLEENDKSKFNAHDLVKFYGNKKRHYA
NVVKTCLINNEPCLKVSWIFPEEAQRPDLQFVKECDMVKVDDQELEFKYF
EGVIITPYPILTIETTNSEYIKINDNVYFILRDSLKKIKGNVVWIGKING
MFVGILNLKRDYELKIGGKFRIKEHRMKYFKEFQYSNLLYIVPFCHLKRE
KYKELQEIIIEFLRSNDDCLGVVINKAMEMREKSVLPLLIENMEVTVKLE
LYQELLLLLFERNQFETMQILLNKSIGNESIDLLLLDIGLKCLKLDKMNI
FESLVQKCTNKNIKFDNGETFLTNIFKENMSNDVIIHLLDMNIFDLNQSN
DGNETPLILACDKLMIDVVEKIMSYSNLNTFQIVYAQWKVLEKNKIDNPI
EEKDKIINMVKMLSSKSFNEKFLSSICSICKEQTTTNVREFESPICENCL
EHKSQSEIKNA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR036770Ankyrin_rpt-contain_sf
IPR020683Ankyrin_rpt-contain_dom
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 450..645
e-value: 8.8E-6
score: 27.4
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 491..628