SMED30002123

Overview
NameSMED30002123
Smed IDSMED30002123
Length (bp)1732
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30002123 (SMED30002123) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30002123 (SMED30002123) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002123

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30002123 SmedASXL_071809SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30002123 SmedASXL_071809SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30002123 Contig14405newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002123 Contig14405uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30002123 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.018159:8106..9502 -6660
v31.003365:12331..14637 -6649
v31.020792:4453..6783 -6455
Homology
[Close]
BLAST of SMED30002123 vs. Ensembl Human
Match: GTF2IRD2 (GTF2I repeat domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30775])

HSP 1 Score: 77.0258 bits (188), Expect = 3.865e-14
Identity = 47/153 (30.72%), Postives = 81/153 (52.94%), Query Frame = -2 
Query:  322 YYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFH---------CIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 753
            + ++VS+A+ G  +M   + G+    +  L  R+  F          CIIH E LC QK  ++   VM++V+K +N I +  L   +    L E++SQY +LL + +++WLSR  VL+RF   L +I +F + +     +L    W++ L F+
Sbjct:  610 WSKLVSVASTGTPAMVDANNGL----VTKLKSRVATFCKGAELKSICCIIHPESLCAQKLKMD--HVMDVVVKSVNWICSRGLNHSEFTTLLYELDSQYGSLLYYTEIKWLSRGLVLKRFFESLEEIDSFMSSRGKPLPQLSSIDWIRDLAFL 756

HSP 2 Score: 21.557 bits (44), Expect = 3.865e-14
Identity = 8/16 (50.00%), Postives = 12/16 (75.00%), Query Frame = -1 
Query:  272 DITKKLNELNVKLKGN 319
            D+T  LN LN+ L+G+
Sbjct:  758 DMTMHLNALNISLQGH 773
[Close]
BLAST of SMED30002123 vs. Ensembl Human
Match: GTF2IRD2 (GTF2I repeat domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30775])

HSP 1 Score: 76.6406 bits (187), Expect = 4.840e-14
Identity = 47/153 (30.72%), Postives = 81/153 (52.94%), Query Frame = -2 
Query:  322 YYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFH---------CIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 753
            + ++VS+A+ G  +M   + G+    +  L  R+  F          CIIH E LC QK  ++   VM++V+K +N I +  L   +    L E++SQY +LL + +++WLSR  VL+RF   L +I +F + +     +L    W++ L F+
Sbjct:  772 WSKLVSVASTGTPAMVDANNGL----VTKLKSRVATFCKGAELKSICCIIHPESLCAQKLKMD--HVMDVVVKSVNWICSRGLNHSEFTTLLYELDSQYGSLLYYTEIKWLSRGLVLKRFFESLEEIDSFMSSRGKPLPQLSSIDWIRDLAFL 918

HSP 2 Score: 21.557 bits (44), Expect = 4.840e-14
Identity = 8/16 (50.00%), Postives = 12/16 (75.00%), Query Frame = -1 
Query:  272 DITKKLNELNVKLKGN 319
            D+T  LN LN+ L+G+
Sbjct:  920 DMTMHLNALNISLQGH 935
[Close]
BLAST of SMED30002123 vs. Ensembl Human
Match: GTF2IRD2B (GTF2I repeat domain containing 2B [Source:HGNC Symbol;Acc:HGNC:33125])

HSP 1 Score: 76.6406 bits (187), Expect = 5.061e-14
Identity = 47/150 (31.33%), Postives = 79/150 (52.67%), Query Frame = -2 
Query:  322 MVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFH---------CIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 744
            +VS+A+ G  +M   + G+    +  L  R+  F          CIIH E LC QK  ++   VM++V+K +N I +  L   +    L E++SQY +LL + +++WLSR  VL+RF   L +I +F + +     +L    W++ L F+
Sbjct:  613 LVSVASTGTPAMVDANNGL----VTKLKSRVATFCKGAELKSICCIIHPESLCAQKLKMD--HVMDVVVKSVNWICSRGLNHSEFTTLLYELDSQYGSLLYYTEIKWLSRGLVLKRFFESLEEIDSFMSSRGKPLPQLSSIDWIRDLAFL 756

HSP 2 Score: 21.557 bits (44), Expect = 5.061e-14
Identity = 8/16 (50.00%), Postives = 12/16 (75.00%), Query Frame = -1 
Query:  272 DITKKLNELNVKLKGN 319
            D+T  LN LN+ L+G+
Sbjct:  758 DMTMHLNALNISLQGH 773
[Close]
BLAST of SMED30002123 vs. Ensembl Human
Match: GTF2IRD2B (GTF2I repeat domain containing 2B [Source:HGNC Symbol;Acc:HGNC:33125])

HSP 1 Score: 76.2554 bits (186), Expect = 6.336e-14
Identity = 47/150 (31.33%), Postives = 79/150 (52.67%), Query Frame = -2 
Query:  322 MVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFH---------CIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 744
            +VS+A+ G  +M   + G+    +  L  R+  F          CIIH E LC QK  ++   VM++V+K +N I +  L   +    L E++SQY +LL + +++WLSR  VL+RF   L +I +F + +     +L    W++ L F+
Sbjct:  780 LVSVASTGTPAMVDANNGL----VTKLKSRVATFCKGAELKSICCIIHPESLCAQKLKMD--HVMDVVVKSVNWICSRGLNHSEFTTLLYELDSQYGSLLYYTEIKWLSRGLVLKRFFESLEEIDSFMSSRGKPLPQLSSIDWIRDLAFL 923

HSP 2 Score: 21.557 bits (44), Expect = 6.336e-14
Identity = 8/16 (50.00%), Postives = 12/16 (75.00%), Query Frame = -1 
Query:  272 DITKKLNELNVKLKGN 319
            D+T  LN LN+ L+G+
Sbjct:  925 DMTMHLNALNISLQGH 940
[Close]
BLAST of SMED30002123 vs. Ensembl Human
Match: FAM200B (family with sequence similarity 200 member B [Source:HGNC Symbol;Acc:HGNC:27740])

HSP 1 Score: 73.9442 bits (180), Expect = 3.944e-13
Identity = 43/140 (30.71%), Postives = 77/140 (55.00%), Query Frame = -2 
Query:  322 SIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNE-KTIVRHELKEDKWLKKLNFI 738
             I +DG  +MTG H  +   +L+  N+     HC IH+E L  ++ P  ++EV+   +K++N I  ++L    L+ F  EI + + +LL H K++WLS+  +L R     ++I  F  E K+ +    ++D W+ KL ++
Sbjct:  293 GITSDGTATMTGKHSRVIKKLLEVTNNGAVWNHCFIHREGLASREIPQNLMEVLKNAVKVVNFIKGSSLNSRLLETFCSEIGTNHTHLLYHTKIRWLSQGKILSRVYELRNEIHFFLIEKKSHLASIFEDDTWVTKLAYL 432
[Close]
BLAST of SMED30002123 vs. Ensembl Zebrafish
Match: CR392001.3 (pep chromosome:GRCz11:8:38963323:38965260:-1 gene:ENSDARG00000117159.1 transcript:ENSDART00000181495.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR392001.3)

HSP 1 Score: 160.614 bits (405), Expect = 9.339e-42
Identity = 109/299 (36.45%), Postives = 160/299 (53.51%), Query Frame = -2 
Query:  322 NDANLTSLTISVQITKRGK*FIDCEYFKDCFISRAEELFSNFKNKKS--------NICHYMLK*YKIEL*HN-TT*HV*LIRXXXXXXXAMDESCNIEDTYTLLFSLG-------ICRLKAQKKN*E--SAALKANSWRR*HKRCAKMRGRL*NPYYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 1164
            N     S   + +I KRGK F D +Y K+ FI+ +E LFS+FKNK           +    +K   I++  N T   +  I  A A S+A DESC+       + S G       +  LK Q +  +   A LK          C    G   N    ++S+ATDGA SM G  +G   ++ + L+  +  FHCI+HQE LC Q FP E + VMNLVI+++N I+   L   Q +  L E++S+Y +LLLHNKV+WLS+  VL RF +CL  +KTF   K ++  +L++ +WL+KL+F+
Sbjct:   99 NSTTAASFVATREIIKRGKPFTDGDYMKESFINISEHLFSDFKNKTEIIQKIKDMPLSAKTVKERAIKMAGNITEQQIKDINSAPAYSIACDESCDTALLCRYVNSDGPQEEIIKLIPLKGQTRGEDICEAVLK----------CLNENGINTN---HLISVATDGAPSMRGSKRGFVTLLQKALDRNLLAFHCILHQEALCAQTFPSECMVVMNLVIEMVNKIIAKALNHRQFRALLDEVDSEYSDLLLHNKVRWLSKDEVLRRFVACLEHVKTFLKSKDLIYPQLEDTEWLEKLHFM 384

HSP 2 Score: 50.8322 bits (120), Expect = 3.683e-6
Identity = 22/40 (55.00%), Postives = 28/40 (70.00%), Query Frame = -3 
Query: 1263 FNQDWTKSFVFICNTDGIPTCHICQEKLEYN-KSNFERYF 1379
            FN  WT+SF F+ N +G+P C +C EKL  N KSN ER+F
Sbjct:   17 FNVSWTESFAFVANAEGLPECLLCSEKLSNNKKSNVERHF 56
[Close]
BLAST of SMED30002123 vs. Ensembl Zebrafish
Match: AL928808.1 (pep chromosome:GRCz11:20:17257101:17259573:-1 gene:ENSDARG00000101333.2 transcript:ENSDART00000166397.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:AL928808.1)

HSP 1 Score: 79.337 bits (194), Expect = 4.928e-15
Identity = 50/144 (34.72%), Postives = 78/144 (54.17%), Query Frame = -2 
Query:  322 EMVSIATDGAKSMTGIHKGITMIILQNLNHRIF-KFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EI-ESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 747
            ++ +I TDGA +M G  +G+  +   +     F  FHCIIHQE    +K  ++   +M  V++I+N + T+ L   Q K  + E+ E    +LL H  V+WLSR +VL RF   L+ +K F  EK     EL + +W+  L F+
Sbjct:  250 KLTAIVTDGAPAMLGSERGLVGLCKADDRFPAFWTFHCIIHQEHWVSKKLNLD--HIMKPVLEIVNFVRTHALNHRQFKNLIDELDEDLPSDLLFHCAVRWLSRGHVLSRFFELLNPVKLFLAEKHKEYPELHDPQWISDLAFL 391
[Close]
BLAST of SMED30002123 vs. Ensembl Xenopus
Match: spag1 (sperm associated antigen 1 [Source:Xenbase;Acc:XB-GENE-853609])

HSP 1 Score: 69.707 bits (169), Expect = 5.720e-13
Identity = 40/107 (37.38%), Postives = 63/107 (58.88%), Query Frame = -2 
Query:  421 IATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTN--TLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERF 735
            I TDGA++M G ++G+    L+        FHCI+HQE+LC     +++ ++M++V K+ N I     +L   + K FL E+++ Y +LLLH  V+WLS    L RF
Sbjct:  256 IVTDGARAMVGRNQGLAGR-LRKEGIDCHMFHCIVHQEVLCGT--SLKMADIMDVVTKVTNLIRGGNRSLTHRRFKNFLEELDAAYGDLLLHTNVRWLSAGKCLVRF 359

HSP 2 Score: 25.0238 bits (53), Expect = 5.720e-13
Identity = 11/23 (47.83%), Postives = 14/23 (60.87%), Query Frame = -3 
Query:  762 LPLSKQTRGEDNTNAVQKCVEDY 830
            +PL   T+GED  NAV+  V  Y
Sbjct:  225 VPLHGSTKGEDIYNAVKATVTKY 247
[Close]
BLAST of SMED30002123 vs. Ensembl Xenopus
Match: ENSXETT00000016563.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:4:37379994:37381838:-1 gene:ENSXETG00000009412.1 transcript:ENSXETT00000016563.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 69.3218 bits (168), Expect = 6.147e-13
Identity = 40/107 (37.38%), Postives = 63/107 (58.88%), Query Frame = -2 
Query:  421 IATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTN--TLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERF 735
            I TDGA++M G ++G+    L+        FHCI+HQE+LC     +++ ++M++V K+ N I     +L   + K FL E+++ Y +LLLH  V+WLS    L RF
Sbjct:  256 IVTDGARAMVGRNQGLAGR-LRKEGIDCHMFHCIVHQEVLCGT--SLKMADIMDVVTKVTNLIRGGNRSLTHRRFKNFLEELDAAYGDLLLHTNVRWLSAGKCLVRF 359

HSP 2 Score: 25.0238 bits (53), Expect = 6.147e-13
Identity = 11/23 (47.83%), Postives = 14/23 (60.87%), Query Frame = -3 
Query:  762 LPLSKQTRGEDNTNAVQKCVEDY 830
            +PL   T+GED  NAV+  V  Y
Sbjct:  225 VPLHGSTKGEDIYNAVKATVTKY 247
[Close]
BLAST of SMED30002123 vs. Ensembl Xenopus
Match: ENSXETT00000028445.1 (general transcription factor II-I repeat domain-containing protein 2-like [Source:NCBI gene;Acc:101734469])

HSP 1 Score: 51.9878 bits (123), Expect = 2.267e-6
Identity = 44/148 (29.73%), Postives = 69/148 (46.62%), Query Frame = -2 
Query:  322 VSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCL-----QLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFF---NEKTIVRHELKEDKWLKKLNFI 741
             S+ T+G+KSMT    G++  +L+         HCI+H+E+L      +++ +VM +V+KI N IL    F       + K FL E+ + Y +   H    W S    L RF S   +I  F    N   I+   L  + +L  L F+
Sbjct:  243 TSVMTNGSKSMTAKSLGLSA-LLRKEGADCVVLHCIMHEEMLIGT--LLKMSDVMEVVVKISNFILEKRGFITAVTKKKFKTFLDELSAAYGDFDSHKNAYWSSAGQCLFRFFSLRKEIYLFLKDTNYDPILTESLCNEDFLSSLAFL 387
[Close]
BLAST of SMED30002123 vs. Ensembl Mouse
Match: Gtf2ird2 (GTF2I repeat domain containing 2 [Source:MGI Symbol;Acc:MGI:2149780])

HSP 1 Score: 72.0182 bits (175), Expect = 1.149e-12
Identity = 43/149 (28.86%), Postives = 72/149 (48.32%), Query Frame = -2 
Query:  322 YYEMVSIATDGAKSMTGIHKGITMIILQNL-----NHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 753
            + ++VS+A+ G  +M   + G+   +            +    CIIH E LC QK  + +  VM++V+  +N I +  L        L E++SQY +LL H  ++WL R  VL RF   L +I +F + +     +L    W+  L F+
Sbjct:  604 WSKLVSVASTGTPAMMDANSGLVTKLRARAASCCKGADLKSVRCIIHPEWLCAQK--LRMGHVMDVVVDSVNWICSRGLNHGDFTTLLYELDSQYGSLLYHTALKWLGRGLVLRRFFESLEEIDSFMSSRGKPVPQLSSRDWILDLAFL 750
[Close]
BLAST of SMED30002123 vs. Ensembl Mouse
Match: Zbed5 (zinc finger, BED type containing 5 [Source:MGI Symbol;Acc:MGI:1919220])

HSP 1 Score: 69.3218 bits (168), Expect = 7.561e-12
Identity = 39/141 (27.66%), Postives = 73/141 (51.77%), Query Frame = -2 
Query:  322 SIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHE--LKEDKWLKKLNFI 738
             + TDGA +  G   G   ++L N + +    HC++H + L ++  P +  EVM  V+  +N +  ++L      +   +++     LLLH + +WLSR  VL+R      ++K FFN+K I + E    ++  L+K+ ++
Sbjct:  401 GVCTDGAPATLGCQSGFQRLVL-NESPKAIGAHCMLHLQTLAMKTLPQDFQEVMKSVLSSVNFVKASSLNSRLFLQLCSDLDEPSKTLLLHTEGRWLSRGKVLKRIFELRDELKMFFNQKAIRQFEALFSDNSALQKVAYL 540
[Close]
BLAST of SMED30002123 vs. Ensembl Mouse
Match: Zmym6 (zinc finger, MYM-type 6 [Source:MGI Symbol;Acc:MGI:106505])

HSP 1 Score: 53.5286 bits (127), Expect = 6.619e-7
Identity = 39/143 (27.27%), Postives = 72/143 (50.35%), Query Frame = -2 
Query:  319 VSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEK--TIVRHELKEDKWLKKLNFIG 741
            V   TDGA SMT  +  +   I +   + +   HC IH+E L  +K    + E++    +I++ +  +      L     E+ S++ NL L+ +V+WLSR  +L R      +I+ F N+K   + R+   +++W+ KL ++ 
Sbjct:  808 VGFCTDGAASMTDRYFRLRSKIQEIAKNTVTFTHCFIHREHLAAKKLSPCLHEILLQSSQILSFVKNSASDSQMLTILCEEMGSEHVNLPLNAEVRWLSRGRILTRLFELRHEIEIFLNQKHSDLARY-FHDEEWIAKLAYLA 949
[Close]
BLAST of SMED30002123 vs. Ensembl Mouse
Match: Zmym6 (zinc finger, MYM-type 6 [Source:MGI Symbol;Acc:MGI:106505])

HSP 1 Score: 53.5286 bits (127), Expect = 6.838e-7
Identity = 39/143 (27.27%), Postives = 72/143 (50.35%), Query Frame = -2 
Query:  319 VSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEK--TIVRHELKEDKWLKKLNFIG 741
            V   TDGA SMT  +  +   I +   + +   HC IH+E L  +K    + E++    +I++ +  +      L     E+ S++ NL L+ +V+WLSR  +L R      +I+ F N+K   + R+   +++W+ KL ++ 
Sbjct:  900 VGFCTDGAASMTDRYFRLRSKIQEIAKNTVTFTHCFIHREHLAAKKLSPCLHEILLQSSQILSFVKNSASDSQMLTILCEEMGSEHVNLPLNAEVRWLSRGRILTRLFELRHEIEIFLNQKHSDLARY-FHDEEWIAKLAYLA 1041
[Close]
BLAST of SMED30002123 vs. UniProt/SwissProt
Match: sp|Q86UP8|GTD2A_HUMAN (General transcription factor II-I repeat domain-containing protein 2A OS=Homo sapiens OX=9606 GN=GTF2IRD2 PE=1 SV=3)

HSP 1 Score: 77.0258 bits (188), Expect = 1.704e-13
Identity = 47/153 (30.72%), Postives = 81/153 (52.94%), Query Frame = -2 
Query:  322 YYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFH---------CIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 753
            + ++VS+A+ G  +M   + G+    +  L  R+  F          CIIH E LC QK  ++   VM++V+K +N I +  L   +    L E++SQY +LL + +++WLSR  VL+RF   L +I +F + +     +L    W++ L F+
Sbjct:  610 WSKLVSVASTGTPAMVDANNGL----VTKLKSRVATFCKGAELKSICCIIHPESLCAQKLKMD--HVMDVVVKSVNWICSRGLNHSEFTTLLYELDSQYGSLLYYTEIKWLSRGLVLKRFFESLEEIDSFMSSRGKPLPQLSSIDWIRDLAFL 756

HSP 2 Score: 21.557 bits (44), Expect = 1.704e-13
Identity = 8/16 (50.00%), Postives = 12/16 (75.00%), Query Frame = -1 
Query:  272 DITKKLNELNVKLKGN 319
            D+T  LN LN+ L+G+
Sbjct:  758 DMTMHLNALNISLQGH 773
[Close]
BLAST of SMED30002123 vs. UniProt/SwissProt
Match: sp|Q6EKJ0|GTD2B_HUMAN (General transcription factor II-I repeat domain-containing protein 2B OS=Homo sapiens OX=9606 GN=GTF2IRD2B PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 2.231e-13
Identity = 47/150 (31.33%), Postives = 79/150 (52.67%), Query Frame = -2 
Query:  322 MVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFH---------CIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 744
            +VS+A+ G  +M   + G+    +  L  R+  F          CIIH E LC QK  ++   VM++V+K +N I +  L   +    L E++SQY +LL + +++WLSR  VL+RF   L +I +F + +     +L    W++ L F+
Sbjct:  613 LVSVASTGTPAMVDANNGL----VTKLKSRVATFCKGAELKSICCIIHPESLCAQKLKMD--HVMDVVVKSVNWICSRGLNHSEFTTLLYELDSQYGSLLYYTEIKWLSRGLVLKRFFESLEEIDSFMSSRGKPLPQLSSIDWIRDLAFL 756

HSP 2 Score: 21.557 bits (44), Expect = 2.231e-13
Identity = 8/16 (50.00%), Postives = 12/16 (75.00%), Query Frame = -1 
Query:  272 DITKKLNELNVKLKGN 319
            D+T  LN LN+ L+G+
Sbjct:  758 DMTMHLNALNISLQGH 773
[Close]
BLAST of SMED30002123 vs. UniProt/SwissProt
Match: sp|P0CF97|F200B_HUMAN (Protein FAM200B OS=Homo sapiens OX=9606 GN=FAM200B PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.894e-12
Identity = 43/140 (30.71%), Postives = 77/140 (55.00%), Query Frame = -2 
Query:  322 SIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNE-KTIVRHELKEDKWLKKLNFI 738
             I +DG  +MTG H  +   +L+  N+     HC IH+E L  ++ P  ++EV+   +K++N I  ++L    L+ F  EI + + +LL H K++WLS+  +L R     ++I  F  E K+ +    ++D W+ KL ++
Sbjct:  293 GITSDGTATMTGKHSRVIKKLLEVTNNGAVWNHCFIHREGLASREIPQNLMEVLKNAVKVVNFIKGSSLNSRLLETFCSEIGTNHTHLLYHTKIRWLSQGKILSRVYELRNEIHFFLIEKKSHLASIFEDDTWVTKLAYL 432
[Close]
BLAST of SMED30002123 vs. UniProt/SwissProt
Match: sp|A4IFA3|GT2D2_BOVIN (General transcription factor II-I repeat domain-containing protein 2 OS=Bos taurus OX=9913 GN=GTF2IRD2 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 3.087e-12
Identity = 42/149 (28.19%), Postives = 78/149 (52.35%), Query Frame = -2 
Query:  322 YYEMVSIATDGAKSMTGIHKGI-----TMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 753
            + ++VS+A+ G  +M   + G+     + + +      +    CIIH E LC QK  ++   +M++V+  +N I +  L   +    L E++ QY +LL + +++WLSR  VL+RF   L +I +F + +     +L    W+K L F+
Sbjct:  611 WSKLVSVASTGTPAMVDANDGLVTKLKSKVAMVCKGSDLKSVCCIIHPESLCAQKLKMD--HIMSVVVNAVNWICSRGLNHSEFTTLLYELDCQYGSLLYYTEIKWLSRGLVLKRFFESLEEIDSFMSSRGKPLPQLSSQDWIKDLAFL 757

HSP 2 Score: 21.557 bits (44), Expect = 3.087e-12
Identity = 8/17 (47.06%), Postives = 12/17 (70.59%), Query Frame = -1 
Query:  269 DITKKLNELNVKLKGNG 319
            D+T  LN LN+ L+G+ 
Sbjct:  759 DMTMHLNTLNISLQGHS 775
[Close]
BLAST of SMED30002123 vs. UniProt/SwissProt
Match: sp|Q6R2W3|SCND3_HUMAN (SCAN domain-containing protein 3 OS=Homo sapiens OX=9606 GN=ZBED9 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 5.448e-12
Identity = 45/142 (31.69%), Postives = 74/142 (52.11%), Query Frame = -2 
Query:  319 VSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKED-KWLKKLNFIG 741
            V + +DGA SMTG H  +   I + L       HC IH+E L ++K   E+  V+N ++KI+N I +N+L           +E+ +  LLLH +++WLSR  VL R     +++  F   K  +  +L +D  W  +L ++ 
Sbjct:  966 VGVCSDGAASMTGKHSEVVTQI-KELAPECKTTHCFIHRESLAMKKISAELNSVLNDIVKIVNYIKSNSLNSRLFSLLCDNMEADHKQLLLHAEIRWLSRGKVLSRMFEIRNELLVFLQGKKPMWSQLFKDVNWTARLAYLS 1106
[Close]
BLAST of SMED30002123 vs. TrEMBL
Match: A0A4Y2QT58 (General transcription factor II-I repeat domain-containing protein 2A OS=Araneus ventricosus OX=182803 GN=GTF2IRD2_65 PE=4 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 2.568e-89
Identity = 147/299 (49.16%), Postives = 188/299 (62.88%), Query Frame = -2 
Query:  322 NDANLTSLTISVQITKRGK*FIDCEYFKDCFISRAEELFSNFKNKKS--------NICHYMLK*YKIEL*HNTT*-HV*LIRXXXXXXXAMDESCNIEDTYTLLFS---------LGICRLKAQKKN*ESAALKANSWRR*HKRCAKMRGRL*NPYYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 1164
            N+ NL S  +S++I KRGK F D EY KDCFI  +EELF +FKNK           +    ++    ++  N T   V  I+LASALSLA+DESC+I  T  + +          LG+  L  Q +  + A           ++C K  G   N   + VSIATDGA+SMTGIH+G+T I+ + +NH I  FHCIIHQE LC Q FP EIVEVMNLVIKIINSIL   L+  Q K+FL EI+SQ+ +LLLHNKV+WLSR NVL+RF   LS+IKTF NEK+I   +L+E KWL+K NF+
Sbjct:   98 NNVNLASFAVSLEIAKRGKPFTDGEYVKDCFIRASEELFRDFKNKAEIMKKIKDLPLSAKTVQDRTAKMSSNVTHMQVEDIQLASALSLAIDESCDILATLFVRYMSSRGPKEELLGLLPLSGQTRGEDIANAV--------QKCLKDNGIDIN---KTVSIATDGARSMTGIHRGVTSILQKKINHEILTFHCIIHQEALCAQTFPAEIVEVMNLVIKIINSILVKELYHRQFKDFLEEIDSQFSDLLLHNKVRWLSRRNVLQRFALNLSEIKTFLNEKSIDHPKLEEHKWLQKFNFM 385

HSP 2 Score: 75.485 bits (184), Expect = 2.568e-89
Identity = 35/49 (71.43%), Postives = 42/49 (85.71%), Query Frame = -3 
Query:  108 LDKVVCFENNLLPFVKDTESGKLLHFENLKQYRDETNATIDTNYFSMAI 254
            L++VVCFE N L  + D E+GKLLHF+NLKQYRDETNATIDTNY S+A+
Sbjct:  410 LEEVVCFEKNYLFLLVDMENGKLLHFKNLKQYRDETNATIDTNYCSIAL 458

HSP 3 Score: 74.7146 bits (182), Expect = 2.568e-89
Identity = 41/78 (52.56%), Postives = 50/78 (64.10%), Query Frame = -3 
Query: 1167 FNQDWTKSFVFICNTDGIPTCHICQEKLEYN-KSNFERYFTKK------TLSNRRREEKSC*RASKTKKXSNSMLSNF 1379
            FNQDWTKSF FI NTDG+PTC IC EKL +N KSN ER+FT K              +K+     KT++ S+SMLSN+
Sbjct:   16 FNQDWTKSFAFIYNTDGLPTCLICHEKLVHNTKSNLERHFTTKHTQFAGKYPTGDARKKAVEELQKTQQQSSSMLSNW 93
[Close]
BLAST of SMED30002123 vs. TrEMBL
Match: A0A4Y2EE01 (General transcription factor II-I repeat domain-containing protein 2A OS=Araneus ventricosus OX=182803 GN=GTF2IRD2_509 PE=4 SV=1)

HSP 1 Score: 223.787 bits (569), Expect = 1.968e-75
Identity = 139/294 (47.28%), Postives = 184/294 (62.59%), Query Frame = -2 
Query:  322 NLTSLTISVQITKRGK*FIDCEYFKDCFISRAEELFSNFKNKKS--------NICHYMLK*YKIEL*HNTT*-HV*LIRXXXXXXXAMDESCNIEDTYTLLFSLGICRLKAQKKN*E-------SAALKANSWRR*HKRCAKMRGRL*NPYYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 1155
            NL S  +S+++ KRGK F D EY KDC I  +EELF +FKNK           +    ++    ++  N T   V  I+LASALSLA+D SC+I+DT  +  +L +  + +Q    E       S   +        ++C +  G   N   ++VSIA DGA+SMTGIH+G+T I+ + +N  I  F CIIHQE LC Q FP EIVEVMNLVIKIINSILT  L+  Q ++FL EI+SQ+ +LLLHNKV+W SR NVL+R   CLS+IKTF NEK+    EL+EDKWL+K NF+
Sbjct:   13 NLASFAVSLEVAKRGKPFTDGEYAKDCVIRASEELFRDFKNKAEIMKKIKDLPLSAKTVQDRTAKMSSNVTHMQVEDIQLASALSLAIDVSCDIKDTTQV--TLFVRYMSSQGPKEELLGLLPLSGQTRGEDIANAVQKCLEGNGIDIN---KIVSIANDGARSMTGIHRGVTSILQKKINLEILTFRCIIHQEALCAQTFPAEIVEVMNLVIKIINSILTKALYHRQFRDFLEEIDSQFSDLLLHNKVRWFSRGNVLQRSALCLSEIKTFLNEKSADHPELEEDKWLQKFNFM 301

HSP 2 Score: 90.5077 bits (223), Expect = 1.968e-75
Identity = 41/57 (71.93%), Postives = 49/57 (85.96%), Query Frame = -3 
Query:   96 TQPMLDKVVCFENNLLPFVKDTESGKLLHFENLKQYRDETNATIDTNYFSMAINKMK 266
            T  +L++VVCFE  LL FV+D ESGKLLHF+NLKQYRDETNATIDTNYFS+A+  M+
Sbjct:  322 TYALLEEVVCFEKTLLLFVEDIESGKLLHFKNLKQYRDETNATIDTNYFSIALKNMR 378
[Close]
BLAST of SMED30002123 vs. TrEMBL
Match: A0A4Y2UQ47 (General transcription factor II-I repeat domain-containing protein 2A OS=Araneus ventricosus OX=182803 GN=GTF2IRD2_428 PE=4 SV=1)

HSP 1 Score: 235.728 bits (600), Expect = 4.944e-75
Identity = 149/318 (46.86%), Postives = 196/318 (61.64%), Query Frame = -2 
Query:  322 NDANLTSLTISVQITKRGK*FIDCEYFKDCFISRAEELFSNFKNKKS---------------------NICHYMLK*YKIEL*HNTT*-HV*LIRXXXXXXXAMDESCNIEDTYTL-LFS------------LGICRLKAQKK--N*ESAALKANSWRR*HKRCAKMRGRL*NPYYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 1164
            N+ NL SL +S++I KRGK F D EY KDCFI  +EELF +FKNK                       ++    ++    ++  N T   V  I+LASALSLA+DESC+++DT  + LF+            LG+  L  Q +  + E+A           ++C +  G   N   ++VSIATDGA+SMTGIH+G+T I+ + +NH I  FHCIIHQE LC Q FP EIVEVMNLVIKIINSIL  +L+  Q K+FL EI+ Q+ +LLLHNKV+W SR NVL+RF  CLS+IK F NEK+I   EL+EDKWL+  NF+
Sbjct:   99 NNVNLVSLAVSLEIAKRGKPFTDGEYVKDCFIRASEELFRDFKNKAEIMKKFFIQSVTSKCVCDIKDLSLSAKTVQDRTAKMSSNVTHMQVEDIQLASALSLAIDESCDVKDTAQVTLFARYMSSQGPKEELLGLLPLSGQTRGEDIENAV----------QKCLEDNGIDIN---KIVSIATDGARSMTGIHRGVTSILQKKINHEILTFHCIIHQESLCAQTFPAEIVEVMNLVIKIINSILAKSLYHRQFKDFLEEIDDQFSDLLLHNKVRWFSRGNVLQRFALCLSEIKAFLNEKSIDHPELEEDKWLQNFNFM 403

HSP 2 Score: 77.0258 bits (188), Expect = 4.944e-75
Identity = 41/78 (52.56%), Postives = 50/78 (64.10%), Query Frame = -3 
Query: 1167 FNQDWTKSFVFICNTDGIPTCHICQEKLEYN-KSNFERYFTKK------TLSNRRREEKSC*RASKTKKXSNSMLSNF 1379
            FNQDWT+SF FICNTDG+PTC IC EKL +N KSN ER+FT K              +K+     K K+ S+SMLSN+
Sbjct:   17 FNQDWTESFAFICNTDGLPTCLICHEKLAHNKKSNLERHFTTKHTQFAGKFPTGDARKKAVEELKKKKQQSSSMLSNW 94
[Close]
BLAST of SMED30002123 vs. TrEMBL
Match: A0A4Y2UIE0 (General transcription factor II-I repeat domain-containing protein 2A (Fragment) OS=Araneus ventricosus OX=182803 GN=GTF2IRD2_61 PE=4 SV=1)

HSP 1 Score: 206.068 bits (523), Expect = 3.553e-74
Identity = 140/313 (44.73%), Postives = 188/313 (60.06%), Query Frame = -2 
Query:  253 NDANLTSLTISVQITKRGK*FIDCEYFKDCFISRAEELFSNFKNKKSNICHYMLK*YKIEL*HNTT*HV*LIRXXXXXXXAMDESCNIEDTYTLLFSLGICRLKAQKKN*ESAAL-------KANSWRR*HKRCAKMRGRL*NPYYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNF-IGISQR-N*MS*M*N*KEMGNPAYA 1164
            N+ NL S  + ++I +RGK F D +        R  ++        SN+ H  ++                I+LASALSLA+DESC+I+DT  +  +L +  + +Q    E   L       +        ++C +  G   N   ++VSIATDGA+ MTGIH+G+T I+ + +NH I  FHCIIHQE LCVQ FP EIVEVMNLVIKIINSIL   L+  Q K+FL EI+SQ+ +LLLHNKV+WLSR NVL+RF  CLS+IKTF N K+    EL+EDKWL+K NF + I+ + N ++     K  GNPAYA
Sbjct:  103 NNVNLASFAVLLEIARRGKPFTDVQ-------DRTAKM-------SSNVTHMQVE---------------DIQLASALSLAIDESCDIKDTAQV--TLFVRYMSSQGPKEELLGLLPFLGQTRGEDIANAVQKCLEDNGIDIN---KIVSIATDGARIMTGIHRGVTSILQKKINHEILMFHCIIHQEALCVQTFPAEIVEVMNLVIKIINSILAKALYHRQFKDFLEEIDSQFSDLLLHNKVRWLSRGNVLQRFALCLSEIKTFLNGKSNEHPELEEDKWLQKFNFMVDITMKLNELNLKLQGK--GNPAYA 379

HSP 2 Score: 67.781 bits (164), Expect = 3.553e-74
Identity = 36/54 (66.67%), Postives = 45/54 (83.33%), Query Frame = -1 
Query: 1256 RRRDKLKKKIENLIRIGQSRLCSYATQMAFRLVIFAKKNLNTI-SQILRDTSLK 1414
            ++R++LKKK EN I+IG+ RL SYATQMAFRLV FA KN +TI SQI +DTSL+
Sbjct:    9 KKREELKKKTENSIKIGRRRLHSYATQMAFRLVSFATKNSHTIRSQIWKDTSLQ 62

HSP 3 Score: 57.3806 bits (137), Expect = 3.553e-74
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = -3 
Query:  108 DTESGKLLHFENLKQYRDETNATIDTNYFSMAI 206
            D  S KL HF+NLKQYRDETNATIDTNYFS+A+
Sbjct:  396 DMVSSKLPHFKNLKQYRDETNATIDTNYFSIAL 428
[Close]
BLAST of SMED30002123 vs. TrEMBL
Match: A0A4Y2HGU3 (General transcription factor II-I repeat domain-containing protein 2A OS=Araneus ventricosus OX=182803 GN=GTF2IRD2_89 PE=4 SV=1)

HSP 1 Score: 199.134 bits (505), Expect = 3.652e-74
Identity = 100/165 (60.61%), Postives = 123/165 (74.55%), Query Frame = -2 
Query:  253 EMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFIGISQRN*MS*M*N*KEMGNPAYA 747
            ++VSI TDGA+SMTGIHKG+T+I+ + +NH I  FHCIIHQE LC Q  P EIVEVMNLVIKIINSIL   L   Q K+FL EI+SQ+ +LLLHNKV+WLSR NVL+RF  CLS+IKTF NEK+I   EL++DKWL+K NF+  +     +     +  GNPAYA
Sbjct:  103 KIVSIETDGARSMTGIHKGVTLILQKKINHEILTFHCIIHQEALCAQTVPAEIVEVMNLVIKIINSILAKALCHRQFKDFLVEIDSQFSDLLLHNKVRWLSRGNVLQRFALCLSEIKTFLNEKSIGHPELEKDKWLQKFNFMLDTTMKLNALNLKLQGKGNPAYA 267

HSP 2 Score: 85.5001 bits (210), Expect = 3.652e-74
Identity = 39/51 (76.47%), Postives = 46/51 (90.20%), Query Frame = -3 
Query:  108 PMLDKVVCFENNLLPFVKDTESGKLLHFENLKQYRDETNATIDTNYFSMAI 260
             +L++VVCFE  LL FV+DTES KLLHF+NLKQYRDETNATIDTNYFS+A+
Sbjct:  267 ALLEEVVCFEKKLLLFVEDTESSKLLHFKNLKQYRDETNATIDTNYFSIAL 317

HSP 3 Score: 37.3502 bits (85), Expect = 3.652e-74
Identity = 21/37 (56.76%), Postives = 23/37 (62.16%), Query Frame = -3 
Query:  744 PKRRIRNL-PLSKQTRGEDNTNAVQKCVEDYEIHIMK 851
            PK  +  L PL  QTR ED  NAVQKC+ED  I I K
Sbjct:   67 PKEELLGLFPLLGQTRVEDVANAVQKCLEDNGIDINK 103

HSP 4 Score: 29.6462 bits (65), Expect = 3.652e-74
Identity = 25/72 (34.72%), Postives = 34/72 (47.22%), Query Frame = -1 
Query:  836 QKIKYLPLYAKMIQDRT------VTQYNITRVAH*TGLCFITCYG*VLQXXXXXXXXXXXRYMSSQSPKEEL 1033
            +K+K LPL AK +QDRT      VT   +  +   + L         ++      + V   YMSSQ PKEEL
Sbjct:    2 KKMKDLPLSAKTVQDRTAKMSSYVTHMQVEDIQLASALSLAIDESCAIKDTAQGTLFVR--YMSSQGPKEEL 71
[Close]
BLAST of SMED30002123 vs. Ensembl Cavefish
Match: ENSAMXT00000049756.1 (pep primary_assembly:Astyanax_mexicanus-2.0:21:10276151:10280466:-1 gene:ENSAMXG00000035254.1 transcript:ENSAMXT00000049756.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 114.005 bits (284), Expect = 1.904e-26
Identity = 58/141 (41.13%), Postives = 84/141 (59.57%), Query Frame = -2 
Query:  319 SIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFC-LQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFIG 738
            ++ TDGA SM G  KG   +I + + H I K HCIIHQE LC +    +  +VM  V K+IN ++  +     Q +  L E++S+Y +L LH+ V+WLS   VLERF SC+  IK F  EK     +L+++KW+ KL F+ 
Sbjct:  244 AVTTDGAPSMVGKQKGAVKLIEEKVGHPIMKLHCIIHQENLCAKMSNSDFNDVMATVAKVINFLVKRSALTHRQFRSLLEEMDSEYADLPLHSAVRWLSCGKVLERFVSCIDAIKVFLAEKGQQYPQLEDEKWIVKLFFLA 384
[Close]
BLAST of SMED30002123 vs. Ensembl Cavefish
Match: ENSAMXT00000031692.1 (pep primary_assembly:Astyanax_mexicanus-2.0:7:21501471:21504188:-1 gene:ENSAMXG00000043766.1 transcript:ENSAMXT00000031692.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 100.523 bits (249), Expect = 1.822e-23
Identity = 54/141 (38.30%), Postives = 80/141 (56.74%), Query Frame = -2 
Query:  319 SIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFC-LQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFIG 738
            ++ TDGA SM G  KG   +I + + H + K HCIIHQE LC +    +  +VM  V K+IN ++  +     Q +  L E++S+Y +L LH  V+WLS   VLERF SC+  IK F  EK     +L+++  + K  F+ 
Sbjct:   84 AVTTDGAPSMVGKQKGAVKLIEEKVGHPVMKLHCIIHQENLCAKMSNSDFNDVMATVAKVINFLVKRSALTHRQFRSLLEEMDSEYADLPLHLAVRWLSCGKVLERFVSCIDAIKVFLAEKGQQYPQLEDENCIVKHFFLA 224

HSP 2 Score: 29.261 bits (64), Expect = 1.822e-23
Identity = 16/37 (43.24%), Postives = 24/37 (64.86%), Query Frame = -1 
Query:  218 DITKKLNELNVKLKGNG*PSLCLIK---LFVSKIIYF 319
            DIT +LNELN++L+G G   L + +    FV+K+  F
Sbjct:  225 DITGQLNELNLRLQGAGQTVLDMFETWTAFVTKLAVF 261
[Close]
BLAST of SMED30002123 vs. Ensembl Cavefish
Match: ENSAMXT00000039226.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000099.1:564912:568121:1 gene:ENSAMXG00000042173.1 transcript:ENSAMXT00000039226.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 102.449 bits (254), Expect = 6.571e-23
Identity = 91/291 (31.27%), Postives = 137/291 (47.08%), Query Frame = -2 
Query:  322 TSLTISVQITKRGK*FIDCEYFKDCFISRAEELFSNFKNK---KSNICHYMLK*Y----KIEL*HNTT*HV*L--IRXXXXXXXAMDESCNIEDTYTLLFSLGICRLKAQKKN*ESAALKANSWRR*HK-----RCAKMRGRL*NPYYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIF-KFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 1149
             S  +S  + K  K F D E FK+  +  ++ LF +FKNK   ++ I +  L       ++EL         L  +      SL  DES +I DT  L+  + +    A  K      L  N   R        R       L  P+ ++VSI  DGA +M GIH G   +  ++     F  +HC+IHQ+ L  +   V+   VM +V+KI+NSI    L     K  L E++++Y +L+LH  V+WLSR  VL+RF + L +I +F   +     EL +D WL  L F+
Sbjct:   96 ASFRVSHLLAKHKKTFTDGELFKEAMLFASDSLFRDFKNKDEIRTAISNMSLGPATVVRRVELLSEDISKQVLSDLSRCEYFSLQFDESVDITDTAQLVVFVRMTFSDASVKEDFLTLLPLNERTRGEDIYRAFRTYATENDL--PFQKLVSITRDGAPAMCGIHAGFIALCRKDPIFPAFVSYHCVIHQQALASKV--VDFSHVMTVVVKIVNSIRAKALQHRLFKSLLDELDAEYGDLILHADVRWLSRGKVLQRFINLLPEIISFLKSRKEEYRELADDTWLLDLAFL 382

HSP 2 Score: 25.409 bits (54), Expect = 6.571e-23
Identity = 14/34 (41.18%), Postives = 19/34 (55.88%), Query Frame = -1 
Query:  218 DITKKLNELNVKLKGNG*PSLCLIKLFVSKIIYF 319
            D+T KLNELN +L+G      C +   VS +  F
Sbjct:  384 DLTSKLNELNCELQGKD----CDVPHMVSAVNAF 413
[Close]
BLAST of SMED30002123 vs. Ensembl Cavefish
Match: ENSAMXT00000030525.1 (pep primary_assembly:Astyanax_mexicanus-2.0:1:12992682:12994541:1 gene:ENSAMXG00000035573.1 transcript:ENSAMXT00000030525.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 92.8189 bits (229), Expect = 8.063e-20
Identity = 51/145 (35.17%), Postives = 80/145 (55.17%), Query Frame = -2 
Query:  322 PYYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 756
            P+   V I TDGA SMTG   G+    ++         HCIIHQ+ LC +    +   VM++V+K IN I + +L   + +  L E+ES+Y ++L   +V+WLSR N+L+RF    +++K F  +  +    L + KWL  L F+
Sbjct:  293 PWKRFVGITTDGAPSMTGRKNGLEEESVEE----AIALHCIIHQQALCSKCLKFD--NVMSVVVKCINQIRSRSLKHRRFRALLEEMESEYGDVLYFTEVRWLSRGNILKRFFELRAEVKAFMEKDGVAVTVLSDPKWLMDLAFL 431

HSP 2 Score: 24.2534 bits (51), Expect = 8.063e-20
Identity = 11/17 (64.71%), Postives = 13/17 (76.47%), Query Frame = -1 
Query:  269 DITKKLNELNVKLKGNG 319
            DIT +LN LN KL+G G
Sbjct:  433 DITHELNVLNKKLQGQG 449
[Close]
BLAST of SMED30002123 vs. Ensembl Cavefish
Match: ENSAMXT00000037411.1 (pep primary_assembly:Astyanax_mexicanus-2.0:13:18144228:18145385:-1 gene:ENSAMXG00000033115.1 transcript:ENSAMXT00000037411.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 88.5817 bits (218), Expect = 1.045e-18
Identity = 50/148 (33.78%), Postives = 79/148 (53.38%), Query Frame = -2 
Query:  322 YYEMVSIATDGAKSMTGIHKGITMIILQNLNHR----IFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 753
            + ++ SI TDGA  M G  +G+T  + + +  R      + HC+IHQ+ LC +    +   VM +V+  IN I    L   + ++FL E+ES Y ++L + +V+WLSR  VL RF   L +I  F + K     EL + +W   L F+
Sbjct:   35 WSKLASITTDGAPCMVGASRGLTGRVKREMEERGLTAPLQVHCLIHQQALCCKVLKWD--SVMKVVVSCINFIRAKGLKHREFQQFLSELESAYGDVLYYTEVRWLSRGRVLRRFYELLPEINAFLHSKDKTVPELLDPEWKWHLAFL 180

HSP 2 Score: 25.0238 bits (53), Expect = 1.045e-18
Identity = 9/17 (52.94%), Postives = 14/17 (82.35%), Query Frame = -1 
Query:  269 DITKKLNELNVKLKGNG 319
            D+T+ LN LN++L+G G
Sbjct:  182 DVTEMLNSLNLQLQGQG 198
[Close]
BLAST of SMED30002123 vs. Ensembl Medaka
Match: ENSORLT00000033718.1 (general transcription factor II-I repeat domain-containing protein 2 [Source:NCBI gene;Acc:110015985])

HSP 1 Score: 148.673 bits (374), Expect = 2.243e-56
Identity = 66/141 (46.81%), Postives = 99/141 (70.21%), Query Frame = -2 
Query:  322 MVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 744
            +V +A+DGA SMTG  KG   ++ ++L+ ++  FHCI+HQE LC Q FP E  +VM+LVI+I+N I+ N L   Q +  L E++S Y +LLLHNKV+WLSR  VL+RF +CL ++K F + K +   EL++ +W +KL+F+
Sbjct:  247 LVPVASDGAPSMTGAQKGFVALLQKSLDRKLLTFHCILHQEALCAQTFPPECTQVMDLVIQIVNKIMANGLNHRQFRSLLDELDSAYSDLLLHNKVRWLSRGVVLKRFAACLEEVKVFLSNKGLTFPELEQPEWQEKLHFM 387

HSP 2 Score: 43.8986 bits (102), Expect = 2.243e-56
Identity = 21/45 (46.67%), Postives = 28/45 (62.22%), Query Frame = -2 
Query: 1030 NDANLTSLTISVQITKRGK*FIDCEYFKDCFISRAEELFSNFKNK 1164
            N    TS  ++ +I K GK F D EY K+ FI  ++ LFS+FKNK
Sbjct:   96 NSTTCTSFVVAQEIVKHGKPFTDGEYLKETFIKISKHLFSDFKNK 140

HSP 3 Score: 39.6614 bits (91), Expect = 2.243e-56
Identity = 19/43 (44.19%), Postives = 23/43 (53.49%), Query Frame = -3 
Query: 1254 FNQDWTKSFVFICNTDGIPTCHICQEKLEYN-KSNFERYFTKK 1379
            FN  W  SF F  +  G+P C IC E L  + KSN  R+F  K
Sbjct:   14 FNATWADSFAFTADETGLPVCLICGEILANDKKSNVARHFENK 56

HSP 4 Score: 31.5722 bits (70), Expect = 2.243e-56
Identity = 15/26 (57.69%), Postives = 20/26 (76.92%), Query Frame = -1 
Query:  959 QKIKYLPLYAKMIQDRTVTQY-NITR 1033
            QKIK +PL AK++QDR+V    N+TR
Sbjct:  145 QKIKDMPLSAKIVQDRSVNMAENVTR 170

HSP 5 Score: 31.187 bits (69), Expect = 2.243e-56
Identity = 17/47 (36.17%), Postives = 20/47 (42.55%), Query Frame = -3 
Query:  735 R*VYVVSKPKRRIRNLPLSKQTRGEDNTNAVQKCVEDYEIHIMKWFP 875
            R V      +  I  +PL  QTRGED   AV  C+   EI      P
Sbjct:  203 RYVSAAGPQEEMIELIPLKGQTRGEDICEAVLHCLRTKEIKTTHLVP 249

HSP 6 Score: 26.1794 bits (56), Expect = 2.243e-56
Identity = 12/32 (37.50%), Postives = 19/32 (59.38%), Query Frame = -1 
Query:  245 EKIKLHRDITKKLNELNVKLKGNG*PSLCLIK 340
            EK+    D+T  LN LN  L+G G  +L +++
Sbjct:  382 EKLHFMVDMTAHLNTLNTSLQGKGGTALHMLE 413
[Close]
BLAST of SMED30002123 vs. Ensembl Medaka
Match: ENSORLT00000040270.1 (pep primary_assembly:ASM223467v1:5:3656515:3660788:1 gene:ENSORLG00000025153.1 transcript:ENSORLT00000040270.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 128.257 bits (321), Expect = 7.956e-36
Identity = 60/145 (41.38%), Postives = 90/145 (62.07%), Query Frame = -2 
Query:  322 PYYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 756
            P  ++VS+ TDGA  M G ++G   ++ ++   RI  FHCI+HQE LC Q    ++ EVM+LV++++N I+   L   Q K  L E+ + Y  LLLH+ V+WLSR  VL RF +CLS+I+TF   K +   EL + +WL K  ++
Sbjct:  240 PMDKLVSVCTDGAPCMVGKNRGFVALLREHEKRRILSFHCILHQEALCAQMCGEQLGEVMSLVVRVVNFIVARALNDRQFKALLEEVGNSYPGLLLHSNVRWLSRGKVLSRFAACLSEIRTFLERKNVEHPELADTEWLLKFYYL 384

HSP 2 Score: 38.891 bits (89), Expect = 7.956e-36
Identity = 17/36 (47.22%), Postives = 24/36 (66.67%), Query Frame = -3 
Query:  138 VVCFENNLLPFVKDTESGKLLHFENLKQYRDETNAT 245
            V  FEN L  F+ D E+G+LLHFE L +++D   A+
Sbjct:  412 VFAFENRLELFIADIETGRLLHFEKLAEFKDACIAS 447

HSP 3 Score: 23.8682 bits (50), Expect = 7.956e-36
Identity = 10/17 (58.82%), Postives = 13/17 (76.47%), Query Frame = -1 
Query:  269 DITKKLNELNVKLKGNG 319
            D+T  LN LNVK++G G
Sbjct:  386 DMTGHLNHLNVKMQGVG 402
[Close]
BLAST of SMED30002123 vs. Ensembl Medaka
Match: ENSORLT00000027991.1 (pep primary_assembly:ASM223467v1:15:8817619:8823815:1 gene:ENSORLG00000022420.1 transcript:ENSORLT00000027991.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 128.257 bits (321), Expect = 8.089e-36
Identity = 60/145 (41.38%), Postives = 90/145 (62.07%), Query Frame = -2 
Query:  322 PYYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 756
            P  ++VS+ TDGA  M G ++G   ++ ++   RI  FHCI+HQE LC Q    ++ EVM+LV++++N I+   L   Q K  L E+ + Y  LLLH+ V+WLSR  VL RF +CLS+I+TF   K +   EL + +WL K  ++
Sbjct:  240 PMDKLVSVCTDGAPCMVGKNRGFVALLREHEKRRILSFHCILHQEALCAQMCGEQLGEVMSLVVRVVNFIVARALNDRQFKALLEEVGNSYPGLLLHSNVRWLSRGKVLSRFAACLSEIRTFLERKNVEHPELADTEWLLKFYYL 384

HSP 2 Score: 38.891 bits (89), Expect = 8.089e-36
Identity = 17/36 (47.22%), Postives = 24/36 (66.67%), Query Frame = -3 
Query:  138 VVCFENNLLPFVKDTESGKLLHFENLKQYRDETNAT 245
            V  FEN L  F+ D E+G+LLHFE L +++D   A+
Sbjct:  412 VFAFENRLELFIADIETGRLLHFEKLAEFKDACIAS 447

HSP 3 Score: 23.8682 bits (50), Expect = 8.089e-36
Identity = 10/17 (58.82%), Postives = 13/17 (76.47%), Query Frame = -1 
Query:  269 DITKKLNELNVKLKGNG 319
            D+T  LN LNVK++G G
Sbjct:  386 DMTGHLNHLNVKMQGVG 402
[Close]
BLAST of SMED30002123 vs. Ensembl Medaka
Match: ENSORLT00000035623.1 (pep primary_assembly:ASM223467v1:9:5067000:5069708:1 gene:ENSORLG00000026230.1 transcript:ENSORLT00000035623.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 127.872 bits (320), Expect = 9.982e-36
Identity = 60/145 (41.38%), Postives = 90/145 (62.07%), Query Frame = -2 
Query:  322 PYYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 756
            P  ++VS+ TDGA  M G ++G   ++ ++   RI  FHCI+HQE LC Q    ++ EVM+LV++++N I+   L   Q K  L E+ + Y  LLLH+ V+WLSR  VL RF +CLS+I+TF   K +   EL + +WL K  ++
Sbjct:  267 PMDKLVSVCTDGAPCMVGKNRGFVALLREHEKRRILSFHCILHQEALCAQMCGEQLGEVMSLVVRVVNFIVARALNDRQFKALLEEVGNSYPGLLLHSNVRWLSRGKVLSRFAACLSEIRTFLERKNVEHPELADTEWLLKFYYL 411

HSP 2 Score: 39.2762 bits (90), Expect = 9.982e-36
Identity = 17/36 (47.22%), Postives = 24/36 (66.67%), Query Frame = -3 
Query:  138 VVCFENNLLPFVKDTESGKLLHFENLKQYRDETNAT 245
            V  FEN L  F+ D E+G+LLHFE L +++D   A+
Sbjct:  439 VFAFENRLELFIADIETGRLLHFEKLAEFKDACIAS 474

HSP 3 Score: 23.8682 bits (50), Expect = 9.982e-36
Identity = 10/17 (58.82%), Postives = 13/17 (76.47%), Query Frame = -1 
Query:  269 DITKKLNELNVKLKGNG 319
            D+T  LN LNVK++G G
Sbjct:  413 DMTGHLNHLNVKMQGVG 429
[Close]
BLAST of SMED30002123 vs. Ensembl Medaka
Match: ENSORLT00000038486.1 (pep primary_assembly:ASM223467v1:11:14048492:14066451:1 gene:ENSORLG00000022236.1 transcript:ENSORLT00000038486.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 125.176 bits (313), Expect = 4.672e-35
Identity = 60/145 (41.38%), Postives = 88/145 (60.69%), Query Frame = -2 
Query:  322 PYYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 756
            P  ++VS+ TDGA  M G ++G   ++ ++   RI  FHCI+HQE LC Q    +  EVM+LV++++N I+   L   Q K  L E+   Y  LLLH+ V+WLSR  VL RF +CLS+I+TF   K +   EL + +WL K  ++
Sbjct:  147 PMDKLVSVCTDGALCMVGKNRGFVALLREHEKRRILSFHCILHQEALCAQMCGEQFGEVMSLVVRVVNFIVARALNDRQFKALLDEVGYSYPGLLLHSNVRWLSRGTVLSRFAACLSEIRTFLERKNVEHPELADTEWLLKFYYL 291

HSP 2 Score: 39.6614 bits (91), Expect = 4.672e-35
Identity = 16/41 (39.02%), Postives = 26/41 (63.41%), Query Frame = -3 
Query:  123 VVCFENNLLPFVKDTESGKLLHFENLKQYRDETNATIDTNY 245
            V  FE  L  F+ D E+G+LLHFE L +++D+ +  I  ++
Sbjct:  319 VFAFEKRLELFIADIETGRLLHFEKLSKFKDQASHLISCSH 359

HSP 3 Score: 23.8682 bits (50), Expect = 4.672e-35
Identity = 10/17 (58.82%), Postives = 13/17 (76.47%), Query Frame = -1 
Query:  269 DITKKLNELNVKLKGNG 319
            D+T  LN LNVK++G G
Sbjct:  293 DMTGHLNHLNVKMQGVG 309
[Close]
BLAST of SMED30002123 vs. Planmine SMEST
Match: SMESG000011945.1 (SMESG000011945.1)

HSP 1 Score: 177.948 bits (450), Expect = 9.716e-51
Identity = 116/291 (39.86%), Postives = 167/291 (57.39%), Query Frame = -2 
Query:  322 NDANLTSLTISVQITKRGK*FIDCEYFKDCFISRAEELFSNFKNKKSNI--------CHYMLK*YKIEL*HNTT*H-V*LIRXXXXXXXAMDESCNIEDTYTL-LFSLGICRLKAQKKN*ESAALKANSWRR*HKRCAKMRGRL*NPYYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 1164
            ++ N++   +S +I+KR K + D EY K CFI+ +EELF +FKNK  N+            +K   I++  N T   +  ++L S LS A+D+SC+I+DT  + LF+ G           E  A              +   +   P  E+VSI+TDGAKSM G+ KG   I+ + +NH IF +HCI +QE LC Q FP EI +VM LVI IINSI+   L   QLKE L E+ES+Y + LLHNK+QWLSR N L+R  S L +I+ F  EK +   EL +++ ++  +F+
Sbjct:    5 SNVNISGFVVSQEISKRKKPYTDKEYIKSCFINASEELFRDFKNKADNLKKIKELSLSAKTMKDRTIKMCSNITIQQIEDLKLVSGLSTAVDKSCDIKDTMQVSLFTRG-----------EDIA----------SSVVECMDKYDIPLDEIVSISTDGAKSMIGVSKGFVAILKEKINHEIFVYHCIFNQETLCAQTFPEEICKVMRLVITIINSIVAKALNHRQLKECLVEMESEYADPLLHNKIQWLSRGNALKRLASLLQEIEVFLLEKGVHYPELTDNQRIQNFHFV 274
[Close]
BLAST of SMED30002123 vs. Planmine SMEST
Match: SMESG000011571.1 (SMESG000011571.1)

HSP 1 Score: 145.591 bits (366), Expect = 1.626e-40
Identity = 76/145 (52.41%), Postives = 101/145 (69.66%), Query Frame = -2 
Query:  322 PYYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 756
            P  ++VSI+TD AKSM+G  K    I+ + +NH IF +HCIIHQE LC Q FP EI +VM L+I IINSI+   L   Q KEFL E+ES+Y NLLLHNKV+WLSR NVL+ F+S L +I+ F  EK +   EL  ++W++  +F+
Sbjct:    7 PLDKIVSISTDVAKSMSGFRKWFVAILKERINHEIFAYHCIIHQEALCEQTFPEEISKVMRLMITIINSIVVKGLNHRQFKEFLVEMESEYANLLLHNKVRWLSRRNVLKLFSSLLPEIEVFLLEKGVHYPELTNNQWIQYCHFV 151
[Close]
BLAST of SMED30002123 vs. Planmine SMEST
Match: SMESG000011470.1 (SMESG000011470.1)

HSP 1 Score: 81.2629 bits (199), Expect = 2.389e-17
Identity = 49/130 (37.69%), Postives = 74/130 (56.92%), Query Frame = -2 
Query:  352 SIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTN-TLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKE 738
            +++TD A ++ G   G+  +  + + H I KFHCII+QE +C +   +++ +VM+ V KI+N  +   +L   Q +  L ++ES Y  + LH KV WLS S VLE F  C   IK F  EK     ELK+
Sbjct:   71 AVSTDCAPAIVGRLNGLNNLFEEKIVHPILKFHCIIYQENICAKISKIDVNKVMSTVSKIVNIFMACFSLTKRQFEALLQDMESTYQYISLHCKVIWLSCSKVLEMFVGCFDSIKVFLEEKCERFPELKD 200
[Close]
BLAST of SMED30002123 vs. Planmine SMEST
Match: SMESG000037770.1 (SMESG000037770.1)

HSP 1 Score: 72.4034 bits (176), Expect = 5.181e-15
Identity = 90/294 (30.61%), Postives = 138/294 (46.94%), Query Frame = -2 
Query:  325 ANLTSLTISVQITKRGK*FIDCEYFKDCFISRAEELFSNFKNKKSNIC---HYMLK*YKIEL*HNTT*HV*L--IRXXXXXXXAMDESCNIEDTYTLL-FSLGIC-RLKAQKKN*ESAALK-ANSWRR*HKRCAKMRGRL*NPYYEMVSIATDGAKSMTGIHKGITMII---LQNL--NHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIV---RHELKEDKWLKKLNF 1158
            A   SL I+ +I K  K F + E+ KDC +  A+ +    K K  NI      +++  +IE          L  I+     S+A+DES +I+DT  LL F  GI  +  A ++      LK   + +       K   +   P  ++ S+ TDGA S+TG + G+   I   ++NL  +H I   HCIIHQE LC          V++      N++LT     LQ K FL ++ES + ++L H  V+WLS   VL+R  +   +I  F   K I      ++K  +WL   +F
Sbjct:  618 ATKASLVIAHKIVKHNKPFSEGEFVKDCVLEIADIICPENKKKFENISLSRRTVVR--RIESIAEDLSDQLLNKIKTFQWFSIALDESTDIQDTAQLLIFIRGIDDKFIATEELLSMEHLKDTTTGQDLFDNLIKALDKFELPLDKLASVTTDGAPSLTGKNVGLITKIKDHVKNLHPDHTIIPLHCIIHQESLCKS--------VLDF-----NTLLT----LLQFKSFLEDLESDHSDVLYHTNVRWLSLGKVLKRVWNLKDEIVLFLEMKDITVEFTTQMKTSEWLSDFSF 892

HSP 2 Score: 27.7202 bits (60), Expect = 5.181e-15
Identity = 13/17 (76.47%), Postives = 14/17 (82.35%), Query Frame = -1 
Query:  269 DITKKLNELNVKLKGNG 319
            DI  KLNELNVKL+G G
Sbjct:  895 DILDKLNELNVKLQGKG 911
[Close]
BLAST of SMED30002123 vs. Planmine SMEST
Match: SMESG000025120.1 (SMESG000025120.1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.788e-14
Identity = 51/145 (35.17%), Postives = 76/145 (52.41%), Query Frame = -2 
Query:  322 PYYEMVSIATDGAKSMTGIHKGITMIILQNLNHRIFKFHCIIHQELLCVQKFPVEIVEVMNLVIKIINSILTNTLFCLQLKEFL*EIESQYFNLLLHNKVQWLSRSNVLERFNSCLSKIKTFFNEKTIVRHELKEDKWLKKLNFI 756
            P +++ +I TDGA +M G  K + +   + +NH       I+HQE LC +  P E    + +V KIINSI    L     K  L + E +  +L+LH +V WLS+  +L RF S + +IKTF N+      EL +   L  L F+
Sbjct:  370 PLHKLFAITTDGAPAMVGKKKDL-LTSARRMNH-FQTLRPIMHQEALCCKILPFE--HDIKIVTKIINSIRAAPLQHRLFKTLLEDTEDKEHDLILHTEVSWLSKGKILTRFVSLIEEIKTFINDIKENYDELADSHRLIDLRFL 510
The following BLAST results are available for this feature:
BLAST of SMED30002123 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GTF2IRD23.865e-1430.72GTF2I repeat domain containing 2 [Source:HGNC Symb... [more]
GTF2IRD24.840e-1430.72GTF2I repeat domain containing 2 [Source:HGNC Symb... [more]
GTF2IRD2B5.061e-1431.33GTF2I repeat domain containing 2B [Source:HGNC Sym... [more]
GTF2IRD2B6.336e-1431.33GTF2I repeat domain containing 2B [Source:HGNC Sym... [more]
FAM200B3.944e-1330.71family with sequence similarity 200 member B [Sour... [more]
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BLAST of SMED30002123 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002123 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002123 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CR392001.39.339e-4236.45pep chromosome:GRCz11:8:38963323:38965260:-1 gene:... [more]
AL928808.14.928e-1534.72pep chromosome:GRCz11:20:17257101:17259573:-1 gene... [more]
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BLAST of SMED30002123 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
spag15.720e-1337.38sperm associated antigen 1 [Source:Xenbase;Acc:XB-... [more]
ENSXETT00000016563.16.147e-1337.38pep primary_assembly:Xenopus_tropicalis_v9.1:4:373... [more]
ENSXETT00000028445.12.267e-629.73general transcription factor II-I repeat domain-co... [more]
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BLAST of SMED30002123 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Gtf2ird21.149e-1228.86GTF2I repeat domain containing 2 [Source:MGI Symbo... [more]
Zbed57.561e-1227.66zinc finger, BED type containing 5 [Source:MGI Sym... [more]
Zmym66.619e-727.27zinc finger, MYM-type 6 [Source:MGI Symbol;Acc:MGI... [more]
Zmym66.838e-727.27zinc finger, MYM-type 6 [Source:MGI Symbol;Acc:MGI... [more]
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BLAST of SMED30002123 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q86UP8|GTD2A_HUMAN1.704e-1330.72General transcription factor II-I repeat domain-co... [more]
sp|Q6EKJ0|GTD2B_HUMAN2.231e-1331.33General transcription factor II-I repeat domain-co... [more]
sp|P0CF97|F200B_HUMAN1.894e-1230.71Protein FAM200B OS=Homo sapiens OX=9606 GN=FAM200B... [more]
sp|A4IFA3|GT2D2_BOVIN3.087e-1228.19General transcription factor II-I repeat domain-co... [more]
sp|Q6R2W3|SCND3_HUMAN5.448e-1231.69SCAN domain-containing protein 3 OS=Homo sapiens O... [more]
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BLAST of SMED30002123 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4Y2QT582.568e-8949.16General transcription factor II-I repeat domain-co... [more]
A0A4Y2EE011.968e-7547.28General transcription factor II-I repeat domain-co... [more]
A0A4Y2UQ474.944e-7546.86General transcription factor II-I repeat domain-co... [more]
A0A4Y2UIE03.553e-7444.73General transcription factor II-I repeat domain-co... [more]
A0A4Y2HGU33.652e-7460.61General transcription factor II-I repeat domain-co... [more]
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BLAST of SMED30002123 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000049756.11.904e-2641.13pep primary_assembly:Astyanax_mexicanus-2.0:21:102... [more]
ENSAMXT00000031692.11.822e-2338.30pep primary_assembly:Astyanax_mexicanus-2.0:7:2150... [more]
ENSAMXT00000039226.16.571e-2331.27pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000030525.18.063e-2035.17pep primary_assembly:Astyanax_mexicanus-2.0:1:1299... [more]
ENSAMXT00000037411.11.045e-1833.78pep primary_assembly:Astyanax_mexicanus-2.0:13:181... [more]
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BLAST of SMED30002123 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002123 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002123 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002123 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000033718.12.243e-5646.81general transcription factor II-I repeat domain-co... [more]
ENSORLT00000040270.17.956e-3641.38pep primary_assembly:ASM223467v1:5:3656515:3660788... [more]
ENSORLT00000027991.18.089e-3641.38pep primary_assembly:ASM223467v1:15:8817619:882381... [more]
ENSORLT00000035623.19.982e-3641.38pep primary_assembly:ASM223467v1:9:5067000:5069708... [more]
ENSORLT00000038486.14.672e-3541.38pep primary_assembly:ASM223467v1:11:14048492:14066... [more]
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BLAST of SMED30002123 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000011945.19.716e-5139.86SMESG000011945.1[more]
SMESG000011571.11.626e-4052.41SMESG000011571.1[more]
SMESG000011470.12.389e-1737.69SMESG000011470.1[more]
SMESG000037770.15.181e-1530.61SMESG000037770.1[more]
SMESG000025120.11.788e-1435.17SMESG000025120.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002123 ID=SMED30002123|Name=SMED30002123|organism=Schmidtea mediterranea sexual|type=transcript|length=1732bp
AAAATTCCCCATTTTTAAAAAGCCACTAACATTTTTGTTTCAATATTATG
GTATTTGTGTTTGAGTTTGTTTTGAATTACTCAAATCTCTCAAATCTTCA
TTTTGTTTATAGCCATGCTGAAGTAATTCGTATCAATAGTTGCATTCGTT
TCATCGCGATATTGCTTCAGATTTTCGAAATGTAGTAATTTACCACTCTC
CGTGTCTTTGACAAAAGGAAGTAAATTATTTTCGAAACAAACAACTTTAT
CAAGCATAGGCTGGGTTACCCATTTCCTTTCAGTTTTACATTTAGCTCAT
TTAGTTTCTTTGTGATATCCCGATGAAGTTTAATTTTTTCAGCCATTTAT
CTTCTTTCAATTCGTGACGGACAATGGTTTTTTCATTGAAGAATGTTTTT
ATTTTACTCAAGCAAGAATTAAACCGTTCCAATACGTTACTCCTGGAAAG
CCATTGCACTTTATTGTGCAGAAGTAAATTAAAATACTGGCTTTCTATTT
CTTAAAGAAATTCCTTAAGCTGTAGACAGAATAGTGTATTTGTCAAAATG
CTGTTAATGATCTTAATTACCAAGTTCATGACCTCAACTATTTCGACCGG
AAATTTTTGGACACAAAGCAGCTCTTGATGAATTATACAGTGAAACTTGA
AAATTCTGTGGTTTAGGTTTTGCAGAATAATCATTGTTATTCCTTTATGT
ATTCCTGTCATACTTTTGGCTCCATCAGTTGCTATGGAAACCATTTCATA
ATATGGATTTCATAGTCTTCCACGCATTTTTGCACAGCGTTTGTGTTATC
TTCTCCACGAGTTTGCTTTGAGAGCGGCAGATTCCTAATTCTTCTTTTGG
GCTTTGAGACGACATATACCTAACGAAAACAGTAACGTGTACGTGTCTTC
TATGTTGCAAGACTCATCCATAGCAAGTGATAAAGCAGAGGCCAGTCTAA
TGAGCTACACGTGTTATGTTGTATTGTGTTACAGTTCTATCTTGTATCAT
TTTAGCATATAATGGCAAATATTTGATTTTTTGTTTTTGAAATTACTAAA
CAGTTCTTCAGCTCTACTTATGAAACAATCTTTGAAGTACTCACAATCTA
TGAATTATTTCCCTCTTTTTGTAATTTGTACTGATATTGTAAGGCTTGTC
AGATTTGCATCATTTCAAAGTTACTTAGCATAGAATTCGANTTTTTTTTT
GTTTTTGAAGCTCTTTAACAGCTTTTTTCCTCGCGTCTCCGGTTGGATAG
GGTTTTTTTAGTGAAGTATCTCTCAAAATTTGACTTATTGTATTCAAGTT
TTTCTTGGCAAATATGACAAGTCGGAATGCCATCTGTGTTGCATATGAAC
ACAAACGACTTTGTCCAATCCTGATTAAATTCTCAATTTTCTTCTTTAGT
TTATCTCTTCTTCTTACTCGATTCCATTCTGTCGGCGGAGCACTATACTC
AGTTTAGATTATCTAACAAGAAACTTTTAAAAATGAGTTTTTTNGTATTA
GCAAAAGATGTGCATTTTCATTTCTAAAATGTTGTGCCCTAGGCTGCTGT
TATATCACTTTTGCCAGGAGTCGGCTCTGATTACTGCATCAATTTTATTA
AATTCAAATTATTGTGGTAATTTTTCAAGATTATTTATTAATTAAGATCG
AAATGATTTTTNATTATTTTCAATAAANTTGATACAAATTTTATATATAA
GTCATTAATTTAAGAAAAATTACAAATAAAAA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..52