SMED30002094

Overview
NameSMED30002094
Smed IDSMED30002094
Length (bp)1508
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30002094 (SMED30002094) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30002094 (SMED30002094) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30002094 (SMED30002094) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30002094 (SMED30002094) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30002094

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30002094SMESG000003117.1 Contig1178GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30002094SMESG000003117.1 Contig1178GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30002094SMESG000003117.1 Contig1178GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
protonephridiaSMED30002094SMESG000003128.1 SMESG000003117.1 dd_Smed_v4_2294_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30002094SMESG000003128.1 SMESG000003117.1 dd_Smed_v4_2294_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30002094 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001197:6629..8134 +7406
Homology
BLAST of SMED30002094 vs. Planmine SMEST
Match: SMESG000003117.1 (SMESG000003117.1)

HSP 1 Score: 637.491 bits (1643), Expect = 0.000e+0
Identity = 319/319 (100.00%), Postives = 319/319 (100.00%), Query Frame = 3
Query:  156 MEKNEKITFWLNGDYSSSNQGSESINDPLDSIRITXXXXXXXXXXXYVSRGKPKCNKSIPATVTQNKNKISYFMKVLEELDPKNSPTSENPEIISQLFDNNITTVSTYPKFEDRHCPISASKKTPKKRLQDIIMNKFFSKKRIDMDHWLLDFGDTNLIKCCRRGQIGDVEKALKEIDENHQWPLVNHQNAAKETALSIATYQMNEILINLLIANRAYPQIELAMKNNVLHIAADLKYDKERKTFQYLINGIRIYQPENLLPTLQCKNIFNCDPTEILRLHQQASANDYNLCCIFQQRINSTQVLINSLCNRNFCNPEHI 1112
            MEKNEKITFWLNGDYSSSNQGSESINDPLDSIRITSSSESSKSEEEYVSRGKPKCNKSIPATVTQNKNKISYFMKVLEELDPKNSPTSENPEIISQLFDNNITTVSTYPKFEDRHCPISASKKTPKKRLQDIIMNKFFSKKRIDMDHWLLDFGDTNLIKCCRRGQIGDVEKALKEIDENHQWPLVNHQNAAKETALSIATYQMNEILINLLIANRAYPQIELAMKNNVLHIAADLKYDKERKTFQYLINGIRIYQPENLLPTLQCKNIFNCDPTEILRLHQQASANDYNLCCIFQQRINSTQVLINSLCNRNFCNPEHI
Sbjct:    1 MEKNEKITFWLNGDYSSSNQGSESINDPLDSIRITSSSESSKSEEEYVSRGKPKCNKSIPATVTQNKNKISYFMKVLEELDPKNSPTSENPEIISQLFDNNITTVSTYPKFEDRHCPISASKKTPKKRLQDIIMNKFFSKKRIDMDHWLLDFGDTNLIKCCRRGQIGDVEKALKEIDENHQWPLVNHQNAAKETALSIATYQMNEILINLLIANRAYPQIELAMKNNVLHIAADLKYDKERKTFQYLINGIRIYQPENLLPTLQCKNIFNCDPTEILRLHQQASANDYNLCCIFQQRINSTQVLINSLCNRNFCNPEHI 319          
BLAST of SMED30002094 vs. Planmine SMEST
Match: SMESG000003128.1 (SMESG000003128.1)

HSP 1 Score: 145.591 bits (366), Expect = 2.889e-40
Identity = 68/68 (100.00%), Postives = 68/68 (100.00%), Query Frame = -3
Query: 1243 ENVHEEDSNVGSETTCRKQDGEILTPRMRTKSEAMSDVVNRARYEQDRWRGTVKIQRDRVFHDHSILN 1446
            ENVHEEDSNVGSETTCRKQDGEILTPRMRTKSEAMSDVVNRARYEQDRWRGTVKIQRDRVFHDHSILN
Sbjct:  170 ENVHEEDSNVGSETTCRKQDGEILTPRMRTKSEAMSDVVNRARYEQDRWRGTVKIQRDRVFHDHSILN 237          
The following BLAST results are available for this feature:
BLAST of SMED30002094 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002094 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002094 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002094 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002094 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002094 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002094 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002094 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002094 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002094 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002094 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002094 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002094 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002094 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000003117.10.000e+0100.00SMESG000003117.1[more]
SMESG000003128.12.889e-40100.00SMESG000003128.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002094 ID=SMED30002094|Name=SMED30002094|organism=Schmidtea mediterranea sexual|type=transcript|length=1508bp
TGAATATGTTACGTGGATTTGTGTTGTGTCAGTTTCAGTAATTGTGATGA
AGTACAAACGAGGCTGGCATACTGCCATGCTAGACGTCAATTGGTTAAGA
GCAGAGATCATTTAATTCAATATCTATCAAATAACCAACAATTACATCAT
TTTCAATGGAGAAAAATGAAAAAATAACATTTTGGTTGAACGGAGATTAC
TCAAGTTCCAACCAAGGCAGTGAGTCTATAAATGATCCTTTGGACAGTAT
TCGAATCACTAGCAGTTCGGAATCATCTAAAAGTGAAGAAGAATATGTTT
CCCGAGGAAAACCAAAGTGCAACAAGTCAATTCCTGCAACTGTAACCCAA
AATAAAAACAAAATATCATATTTTATGAAAGTTTTGGAAGAATTAGATCC
TAAAAATTCGCCAACGTCTGAAAATCCAGAAATAATCTCGCAATTATTCG
ATAATAATATAACTACTGTATCTACATACCCAAAATTTGAAGATCGGCAT
TGCCCAATATCAGCTAGCAAAAAAACACCAAAAAAACGTCTCCAAGACAT
TATTATGAACAAATTTTTTAGCAAGAAAAGAATAGATATGGATCATTGGT
TACTTGATTTTGGAGACACTAACTTAATAAAATGTTGTCGGCGTGGTCAA
ATAGGTGATGTGGAAAAAGCGTTGAAGGAAATCGATGAAAATCATCAATG
GCCATTGGTCAATCATCAAAACGCTGCCAAGGAAACAGCGCTATCAATTG
CAACCTATCAGATGAACGAGATACTGATAAATTTATTGATCGCCAATAGA
GCGTATCCCCAAATAGAATTGGCCATGAAAAACAATGTGCTACATATTGC
TGCAGATTTAAAATATGATAAAGAACGCAAAACATTTCAATATCTAATCA
ACGGAATACGGATTTACCAGCCTGAAAACCTGTTACCTACTCTACAATGT
AAGAATATATTCAATTGTGACCCAACGGAAATTCTTCGATTACATCAGCA
AGCCAGTGCGAACGACTATAACTTATGCTGCATATTCCAACAGAGAATCA
ATAGCACTCAAGTGCTAATCAATAGTTTATGTAATAGAAATTTCTGTAAC
CCTGAACATATTTAAAATCAGTTATTTATTTTCCTATTATTATTATTTGA
ATAAAAATAAGAAACAAATTGATTTATTTAATGATTAAATGTGATAAAAT
CTATGACAATAATTTCTATTGAATACATGTATTGATCATTCAATTTAATA
TCGAATGATCGTGGAAAACTCGATCACGCTGAATTTTCACAGTTCCACGC
CATCGATCTTGCTCGTAACGAGCCCGATTTACAACATCGGACATCGCCTC
TGATTTGGTTCGCATTCTAGGAGTTAATATTTCTCCGTCCTGTTTCCGGC
AGGTTGTCTCGCTGCCGACATTCGAATCTTCTTCGTGGACATTTTCTAAA
TATTAATAGATTTTTATTATCAAAACTCATAAAGTTTTGAATTATTGATT
ATTTTAAC
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protein sequence of SMED30002094-orf-1

>SMED30002094-orf-1 ID=SMED30002094-orf-1|Name=SMED30002094-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=320bp
MEKNEKITFWLNGDYSSSNQGSESINDPLDSIRITSSSESSKSEEEYVSR
GKPKCNKSIPATVTQNKNKISYFMKVLEELDPKNSPTSENPEIISQLFDN
NITTVSTYPKFEDRHCPISASKKTPKKRLQDIIMNKFFSKKRIDMDHWLL
DFGDTNLIKCCRRGQIGDVEKALKEIDENHQWPLVNHQNAAKETALSIAT
YQMNEILINLLIANRAYPQIELAMKNNVLHIAADLKYDKERKTFQYLING
IRIYQPENLLPTLQCKNIFNCDPTEILRLHQQASANDYNLCCIFQQRINS
TQVLINSLCNRNFCNPEHI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR020683Ankyrin_rpt-contain_dom
IPR008853TMEM9
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..20
score: 0.567