Phosphatidylinositol-glycan biosynthesis class f protein

Overview
NamePhosphatidylinositol-glycan biosynthesis class f protein
Smed IDSMED30002084
Length (bp)659
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Phosphatidylinositol-glycan biosynthesis class f protein (SMED30002084) t-SNE clustered cells

Violin plots show distribution of expression levels for Phosphatidylinositol-glycan biosynthesis class f protein (SMED30002084) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Phosphatidylinositol-glycan biosynthesis class f protein (SMED30002084) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Phosphatidylinositol-glycan biosynthesis class f protein (SMED30002084) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002084

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior sideSMED30002084h1SMcG0004745 dd_Smed_v4_6693_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30002084h1SMcG0004745 dd_Smed_v4_6693_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Human
Match: PIGF (phosphatidylinositol glycan anchor biosynthesis class F [Source:HGNC Symbol;Acc:HGNC:8962])

HSP 1 Score: 51.9878 bits (123), Expect = 1.302e-7
Identity = 35/115 (30.43%), Postives = 56/115 (48.70%), Query Frame = 2 
Query:   92 FLCTILAYHFILIIYGAEILDWKPTLFL-ATWLGVVTLLPFFLTSQLTVSDFLFVILNENIS---KRSNKFTLGQSFARWFTRFAVIGCWVGAWVIPLDWDRPWQIYPWPCFYGS 424
            FL +  ++H I ++YGA +++     FL A  L   T +P        +  +L V     ++   + S + T   SF         +G W+GA  IPLDW+RPWQ++P  C  G+
Sbjct:   87 FLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSF---------VGAWLGALPIPLDWERPWQVWPISCTLGA 192
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Fly
Match: PIG-F (gene:FBgn0036893 transcript:FBtr0074946)

HSP 1 Score: 56.6102 bits (135), Expect = 1.758e-9
Identity = 40/122 (32.79%), Postives = 62/122 (50.82%), Query Frame = 2 
Query:   92 FLCTILAYHFILIIYGAEIL-DWKPTLFLATWLGVVTLLP-FFLTSQLTVSDFLFVILNENISKRSNKFTLGQSFARWFTRFAVIGCWVGAWVIPLDWDRPWQIYPWPCFYGSLIFSFISTI 451
            FLCT+L Y FI II GA +L +++ T  LA  + ++T+ P  FL          F    + ++K  +      +  ++     ++G W G+ V PLDW R WQ YP P   G+L+ S +  I
Sbjct:   99 FLCTLL-YAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGALQVCFCEKPDFVTKCEDT---ALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGALLGSALGNI 216
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Zebrafish
Match: pigf (phosphatidylinositol glycan anchor biosynthesis, class F [Source:ZFIN;Acc:ZDB-GENE-040426-1801])

HSP 1 Score: 61.2326 bits (147), Expect = 4.139e-11
Identity = 42/130 (32.31%), Postives = 63/130 (48.46%), Query Frame = 2 
Query:   62 IRKIFQYISTFLCTILAYHFILIIYGAEILDWK-PTLFLATWLGVVTLLPFFLTSQLTVSDFLFVILNENISKRSNKFTLGQSFARWFTRF------AVIGCWVGAWVIPLDWDRPWQIYPWPCFYGSLI 430
            I + F+    FL + L +H ++++YGA +L+    T  LA  L  +T L                IL  N+      F+   + + W T        +VIG W+GA+ IPLDWDRPWQ++P  C  G+ I
Sbjct:   77 ISRFFRSCLYFLLSCLFFHTVVVLYGAPLLESALETFSLAVLLSTLTTLR------------CLCILGPNVQAWIRVFSRDGAMSVWDTSLQITTGCSVIGAWLGAFPIPLDWDRPWQVWPISCTLGATI 194
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Xenopus
Match: dupd1 (dual specificity phosphatase and pro isomerase domain containing 1 [Source:Xenbase;Acc:XB-GENE-5944519])

HSP 1 Score: 56.6102 bits (135), Expect = 2.658e-9
Identity = 41/140 (29.29%), Postives = 67/140 (47.86%), Query Frame = 2 
Query:   62 IRKIFQYISTFLCTILAYHFILIIYGAEILDWKPTLFLATWLGVVTLLPFFLTSQLTVSDFLFVILNENISKRSNKFTLGQSFARW------FTRFAVIGCWVGAWVIPLDWDRPWQIYPWPCFYGSLIFSFISTIGFVV 463
            + K+ +    F+ +   +H I+++YGA +++     FL        LL  F TS+         +L  N S     F+   + + W       T  +V+G W+GA+ IPLDWDRPWQ++P  C  G       +T+G+V 
Sbjct:   77 LSKLTKSCIYFVISCFLFHGIIVLYGAPLVESVAETFL-----FAVLLSSFTTSRC------LCLLGPNFSAWVRVFSKDGALSVWDHSLQITTVCSVVGAWLGAFPIPLDWDRPWQVWPISCSLG-------ATLGYVA 198
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Mouse
Match: Pigf (phosphatidylinositol glycan anchor biosynthesis, class F [Source:MGI Symbol;Acc:MGI:99462])

HSP 1 Score: 49.2914 bits (116), Expect = 7.444e-7
Identity = 35/125 (28.00%), Postives = 57/125 (45.60%), Query Frame = 2 
Query:   62 IRKIFQYISTFLCTILAYHFILIIYGAEILDWKPTLFL-ATWLGVVTLLPFFLTSQLTVSDFLFVILNENIS---KRSNKFTLGQSFARWFTRFAVIGCWVGAWVIPLDWDRPWQIYPWPCFYGS 424
            + +  +    FL +    H I ++YGA +++     FL A  L   T +P        +  +L V     ++   + S + T   SF          G W+GA+ IPLDW+RPWQ++P  C  G+
Sbjct:   77 VTRALKCCVCFLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFT---------GAWLGAFPIPLDWERPWQVWPISCTLGA 192
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. UniProt/SwissProt
Match: sp|Q6C741|GPI11_YARLI (Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=GPI11 PE=3 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 5.991e-7
Identity = 42/143 (29.37%), Postives = 67/143 (46.85%), Query Frame = 2 
Query:   65 RKIFQYIST-FLCTILAYHFILIIYGAEILDWKPTLFL-------ATWLGVVTLLPFFLTSQLTVSDFLFVILNENISKRSNKFTLGQSFARWFTRFAVIGCWVGAWVIPLDWDRPWQIYPWPCFYGSLIFSFISTIGFVVLN 469
            RKI   +ST  +   L+  F +II+G  +L   P + L       A  + ++ +LP F T  L    +  +I        + +  L   +A   +   +IG W+GA  IP DWDRPWQ +P     G+ +  F+ T+G + L 
Sbjct:   78 RKIGTDVSTAMIAAALSVFFSVIIFGLLVLFGAPAISLVHNTFVCAMHMSILAVLPLFFTYHLDSKVWADII--------AMRRPLDHVYAA--SVCTLIGAWLGAIPIPYDWDRPWQQWPITILAGAYLGYFVGTLGGIALE 210
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. UniProt/SwissProt
Match: sp|Q07326|PIGF_HUMAN (Phosphatidylinositol-glycan biosynthesis class F protein OS=Homo sapiens OX=9606 GN=PIGF PE=1 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 6.252e-7
Identity = 35/115 (30.43%), Postives = 56/115 (48.70%), Query Frame = 2 
Query:   92 FLCTILAYHFILIIYGAEILDWKPTLFL-ATWLGVVTLLPFFLTSQLTVSDFLFVILNENIS---KRSNKFTLGQSFARWFTRFAVIGCWVGAWVIPLDWDRPWQIYPWPCFYGS 424
            FL +  ++H I ++YGA +++     FL A  L   T +P        +  +L V     ++   + S + T   SF         +G W+GA  IPLDW+RPWQ++P  C  G+
Sbjct:   87 FLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSF---------VGAWLGALPIPLDWERPWQVWPISCTLGA 192
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. UniProt/SwissProt
Match: sp|Q6CTT3|GPI11_KLULA (Glycosylphosphatidylinositol anchor biosynthesis protein 11 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=GPI11 PE=3 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 2.352e-6
Identity = 23/46 (50.00%), Postives = 30/46 (65.22%), Query Frame = 2 
Query:  308 FARWFTRFAVIGCWVGAWVIPLDWDRPWQIYPWPCFYGSLIFSFIS 445
            F ++F    VIGCW+  +VIPLDWDR WQ +P P   G+ + SFI 
Sbjct:  166 FKKYFIS-VVIGCWISCFVIPLDWDRDWQAWPVPLIVGAYLGSFIG 210
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. UniProt/SwissProt
Match: sp|O09101|PIGF_MOUSE (Phosphatidylinositol-glycan biosynthesis class F protein OS=Mus musculus OX=10090 GN=Pigf PE=1 SV=1)

HSP 1 Score: 49.2914 bits (116), Expect = 5.207e-6
Identity = 35/125 (28.00%), Postives = 57/125 (45.60%), Query Frame = 2 
Query:   62 IRKIFQYISTFLCTILAYHFILIIYGAEILDWKPTLFL-ATWLGVVTLLPFFLTSQLTVSDFLFVILNENIS---KRSNKFTLGQSFARWFTRFAVIGCWVGAWVIPLDWDRPWQIYPWPCFYGS 424
            + +  +    FL +    H I ++YGA +++     FL A  L   T +P        +  +L V     ++   + S + T   SF          G W+GA+ IPLDW+RPWQ++P  C  G+
Sbjct:   77 VTRALKCCVCFLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFT---------GAWLGAFPIPLDWERPWQVWPISCTLGA 192
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. TrEMBL
Match: A0A329SZT4 (Uncharacterized protein OS=Phytophthora cactorum OX=29920 GN=PC110_g2295 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 2.121e-13
Identity = 43/141 (30.50%), Postives = 72/141 (51.06%), Query Frame = 2 
Query:   65 RKIFQYISTFLCTILAYHFILIIYGAEILD-WKPTLFLATWLGVVTLLPFFLTSQLTVSDFLFVILNENISKRSNKFTLGQSFARWFTRFAVIGCWVGAWVIPLDWDRPWQIYPWPCFYGSLIFSFISTIGFVVLNTLRKY 484
            ++I   I   +   + +H  ++++GA ++D W  TL LA  L     +PF L  +     +L ++L  N+S   + + +  S        A++G +VGA  IPLDWDRPWQ +P  C YG+L+         +V++   KY
Sbjct:   63 KQILGGILGLVMGSIVFHVTVVLFGAPVVDLWMQTLLLAVLLSSCVTMPFALHLECAPRKWLDLLLELNVSNSQDLYLVSSSIG------AMLGAYVGALPIPLDWDRPWQQWPLTCVYGTLMGHAAGIFVSIVISATSKY 197
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. TrEMBL
Match: C3Z3M2 (Uncharacterized protein (Fragment) OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_211692 PE=4 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.258e-12
Identity = 45/133 (33.83%), Postives = 73/133 (54.89%), Query Frame = 2 
Query:   92 FLCTILAYHFILIIYGAEIL-DWKPTLFLATWLGVVTLLPFFLTSQLTVSDFLFVILNENISKRSNKFTLGQSFARWFTRFAVIGCWVGAWVIPLDWDRPWQIYPWPCFYGSLIFSFISTIGFVVLNTLRKYR 487
            FL +IL++H I ++YGA +  D + T   A  +  +T LP +      +  ++ V+LN   S+        +SF +      V+G W+GA+ IPLDWDRPWQ++P PC  G+L    +   G +VL+ ++  R
Sbjct:   12 FLLSILSFHVIAVLYGAPLQEDTEETFSWALLMATLTALPCWCMLGSRLETWVAVVLNRPESEI-------ESFLQTTVLCTVVGAWLGAFPIPLDWDRPWQVWPIPCSIGAL----LGHTGGLVLSAIQAKR 133
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. TrEMBL
Match: A0A225VFX3 (Protein kinase OS=Phytophthora megakarya OX=4795 GN=PHMEG_00023608 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 4.286e-12
Identity = 46/158 (29.11%), Postives = 78/158 (49.37%), Query Frame = 2 
Query:   11 ILNNCLLSF*VIYYILMIRKIFQYISTFLCTILAYHFILIIYGAEILD-WKPTLFLATWLGVVTLLPFFLTSQLTVSDFLFVILNENISKRSNKFTLGQSFARWFTRFAVIGCWVGAWVIPLDWDRPWQIYPWPCFYGSLIFSFISTIGFVVLNTLRK 481
            +L N  L   V+  + ++++I   I      +  +H I++++GA ++D W+ TL LA  L     +P  L        +  ++L  +++ +   + +  SF       A++G +VGA  IPLDWDRPWQ +P  C YG+L       +  VVL    K
Sbjct:   23 LLLNQHLEDGVLSGLSLLKQIAHGILGLALGLPVFHVIIVMFGAPVVDLWERTLLLAVLLSSCVTMPLALHFGCDPRKWQDLLLELSVNNKEELYLVCSSFG------ALVGVYVGALPIPLDWDRPWQQWPLTCVYGTLFGHAAGIVTSVVLEAKSK 174
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. TrEMBL
Match: A0A1D1UUN6 (Uncharacterized protein OS=Ramazzottius varieornatus OX=947166 GN=RvY_02386-1 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 5.705e-12
Identity = 44/112 (39.29%), Postives = 57/112 (50.89%), Query Frame = 2 
Query:   92 FLCTILAYHFILIIYGAEILDWK-PTLFLATWLGVVTLLPFFLTSQLTVSDFLFVILNENISKRSNKFTLGQSFARWFTRFAVIGCWVGAWVIPLDWDRPWQIYPWPCFYGS 424
             L ++L +   +I++GA ILD    TL LA  L     LPF       V + L V+  E +     K  L      +    AV+G W GA VIPLDWDRPWQ +P PC YG+
Sbjct:   92 MLASVLLFFVGIILFGAPILDSSMETLTLAMLLATTAFLPFCYVYAQPVDELLKVLQTEKLDLSMEKAPL------FPGMLAVVGAWCGAVVIPLDWDRPWQAWPIPCCYGA 197
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. TrEMBL
Match: A0A1V9Z2C6 (Uncharacterized protein OS=Achlya hypogyna OX=1202772 GN=ACHHYP_04089 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 8.590e-12
Identity = 47/147 (31.97%), Postives = 78/147 (53.06%), Query Frame = 2 
Query:   62 IRKIFQYISTFLCTILAYHFILIIYGAEILD-WKPTLFLATWLGVVTLLPFFLTSQLTV---SDFLFVILNE---NISKRSNKFTLGQSFARWFTRFAVIGCWVGAWVIPLDWDRPWQIYPWPCFYGSL---IFSFISTIGFVVLNT 472
            +++    +   L ++   H ILI +GA +LD ++ TL LAT+L  + +LP  LTS L +    +++  IL++   N  +R    T   +          +G ++GA ++PLDWDRPWQ +P PC YG++   I   +   G +V  T
Sbjct:   74 VQQAIGSLGLTLASVGVIHVILIFFGAPVLDLFQRTLLLATYLAFMAVLP--LTSALGLVHAHEYVETILHQRWRNHGERQGTCTFVGTL---------LGAYIGALLLPLDWDRPWQQWPLPCVYGAVYGQIAGLVVATGLLVAQT 209
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Cavefish
Match: pigf (phosphatidylinositol glycan anchor biosynthesis class F [Source:NCBI gene;Acc:103043531])

HSP 1 Score: 56.6102 bits (135), Expect = 1.338e-9
Identity = 35/129 (27.13%), Postives = 62/129 (48.06%), Query Frame = 2 
Query:   62 IRKIFQYISTFLCTILAYHFILIIYGAEILDWKPTLFLATWLGVVTLLPFFLTSQLTVSDFLFVILNENISKRSNKFTLGQSFARWFTRF------AVIGCWVGAWVIPLDWDRPWQIYPWPCFYGSLI 430
            + K+ +     L + L +H ++++YGA +L+     F      +  LL  F T +         +L  N+      F+   + + W T        +++G W+GA+ IPLDWDRPWQ++P  C  G+ +
Sbjct:   77 LSKLIRSCVYLLLSCLFFHTVIVLYGAPLLESALETF-----SLAVLLSTFTTLRC------LCVLGPNVQAWIRVFSRDGAMSVWDTSLQITTGCSIVGAWLGAFPIPLDWDRPWQVWPVSCSLGATV 194
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Sea Lamprey
Match: pigf (phosphatidylinositol glycan anchor biosynthesis, class F [Source:ZFIN;Acc:ZDB-GENE-040426-1801])

HSP 1 Score: 49.6766 bits (117), Expect = 3.233e-8
Identity = 35/116 (30.17%), Postives = 52/116 (44.83%), Query Frame = 2 
Query:  101 TILAYHFILIIYGAEILDW-KPTLFLATWLGVVTLLPFFLTSQLTVSDFLFVILNENISKRSNKFTLGQSFARWFTRFAV------IGCWVGAWVIPLDWDRPWQIYPWPCFYGSL 427
            + L +H + I++GA ++   + T   +  L   T LP               IL  N+S     FT   + + W     V      +G W+GA  IPLDWDRPWQ +P  C  G++
Sbjct:   11 SCLFFHAVTILFGAPVIHLARETFVFSILLTTFTALP------------CLCILGPNLSAWLRVFTRSGAVSVWEQGLCVGTMCTLMGAWLGALPIPLDWDRPWQAWPITCSMGAI 114
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Medaka
Match: pigf (phosphatidylinositol glycan anchor biosynthesis class F [Source:NCBI gene;Acc:105357965])

HSP 1 Score: 57.7658 bits (138), Expect = 5.321e-10
Identity = 38/129 (29.46%), Postives = 62/129 (48.06%), Query Frame = 2 
Query:   62 IRKIFQYISTFLCTILAYHFILIIYGAEILDWKPTLFLATWLGVVTLLPFFLTSQLTVSDFLFVILNENISKRSNKFTLGQSFARWFTRFAV------IGCWVGAWVIPLDWDRPWQIYPWPCFYGSLI 430
            + ++F+    F  + L +H ++++YGA +++     F          L   LTS  T+      +L  N+      F+   + + W T   +      IG WVGA+ IPLDWDRPWQ++P  C  G+ I
Sbjct:   77 LSRLFRACLYFFLSCLFFHTVIVLYGAPLIESALETFS---------LAVLLTSLTTMR--CLCVLGPNVQAWIRVFSRHGAMSVWDTCLQITVACTLIGAWVGAFPIPLDWDRPWQVWPVSCSLGATI 194
The following BLAST results are available for this feature:
BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
PIGF1.302e-730.43phosphatidylinositol glycan anchor biosynthesis cl... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
PIG-F1.758e-932.79gene:FBgn0036893 transcript:FBtr0074946[more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pigf4.139e-1132.31phosphatidylinositol glycan anchor biosynthesis, c... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
dupd12.658e-929.29dual specificity phosphatase and pro isomerase dom... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Pigf7.444e-728.00phosphatidylinositol glycan anchor biosynthesis, c... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q6C741|GPI11_YARLI5.991e-729.37Glycosylphosphatidylinositol anchor biosynthesis p... [more]
sp|Q07326|PIGF_HUMAN6.252e-730.43Phosphatidylinositol-glycan biosynthesis class F p... [more]
sp|Q6CTT3|GPI11_KLULA2.352e-650.00Glycosylphosphatidylinositol anchor biosynthesis p... [more]
sp|O09101|PIGF_MOUSE5.207e-628.00Phosphatidylinositol-glycan biosynthesis class F p... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A329SZT42.121e-1330.50Uncharacterized protein OS=Phytophthora cactorum O... [more]
C3Z3M24.258e-1233.83Uncharacterized protein (Fragment) OS=Branchiostom... [more]
A0A225VFX34.286e-1229.11Protein kinase OS=Phytophthora megakarya OX=4795 G... [more]
A0A1D1UUN65.705e-1239.29Uncharacterized protein OS=Ramazzottius varieornat... [more]
A0A1V9Z2C68.590e-1231.97Uncharacterized protein OS=Achlya hypogyna OX=1202... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pigf1.338e-927.13phosphatidylinositol glycan anchor biosynthesis cl... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pigf3.233e-830.17phosphatidylinositol glycan anchor biosynthesis, c... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pigf5.321e-1029.46phosphatidylinositol glycan anchor biosynthesis cl... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class f protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 0
Match NameE-valueIdentityDescription
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002084 ID=SMED30002084|Name=Phosphatidylinositol-glycan biosynthesis class f protein|organism=Schmidtea mediterranea sexual|type=transcript|length=659bp
ATTTGGTTATATTTTAAATAACTGCTTACTAAGTTTTTAAGTTATCTATT
ATATTTTAATGATCAGAAAAATATTCCAATATATTTCAACATTTTTGTGC
ACCATTCTTGCATATCATTTTATTTTAATCATTTATGGAGCAGAAATTCT
TGACTGGAAACCCACTTTATTTTTGGCTACTTGGCTTGGAGTAGTGACAC
TTCTACCATTCTTTTTAACGTCTCAGTTGACAGTTTCTGATTTTTTGTTC
GTAATTCTGAATGAAAATATCTCAAAGAGAAGCAACAAGTTTACTTTGGG
TCAATCATTCGCGCGTTGGTTTACTCGATTTGCTGTAATTGGTTGCTGGG
TAGGTGCTTGGGTTATTCCACTCGATTGGGATAGACCTTGGCAGATTTAT
CCGTGGCCTTGTTTTTACGGTTCATTGATATTTTCATTTATTAGCACTAT
TGGATTTGTTGTTTTAAATACTTTAAGAAAATATCGTCTCTTAATTATAG
GAACTGGCTGTCCTAAAACAGTTTAATAAAAACTAAATCATTTTTATTTT
TTTAATAAAAGCTTGACTTATTTTTCAATTTAGCTGTGAATTATTGATTT
TTATTAGTTCGAGGATTTCGTTTCTGATTAAAATAAAGCTTTCTATACGG
GGGAGATCG
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protein sequence of SMED30002084-orf-1

>SMED30002084-orf-1 ID=SMED30002084-orf-1|Name=SMED30002084-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=156bp
MIRKIFQYISTFLCTILAYHFILIIYGAEILDWKPTLFLATWLGVVTLLP
FFLTSQLTVSDFLFVILNENISKRSNKFTLGQSFARWFTRFAVIGCWVGA
WVIPLDWDRPWQIYPWPCFYGSLIFSFISTIGFVVLNTLRKYRLLIIGTG
CPKTV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0003001lateral region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR009580GPI_biosynthesis_protein_Pig-F
Vocabulary: cellular component
TermDefinition
GO:0005789endoplasmic reticulum membrane
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0006506GPI anchor biosynthetic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009580GPI biosynthesis protein Pig-FPFAMPF06699PIG-Fcoord: 3..129
e-value: 2.1E-21
score: 76.9
NoneNo IPR availablePANTHERPTHR43157:SF12PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEINcoord: 5..133
NoneNo IPR availablePANTHERPTHR43157FAMILY NOT NAMEDcoord: 5..133
NoneNo IPR availableTMHMMTMhelixcoord: 84..103
NoneNo IPR availableTMHMMTMhelixcoord: 5..27
NoneNo IPR availableTMHMMTMhelixcoord: 42..64
NoneNo IPR availableTMHMMTMhelixcoord: 113..135