SMED30002066
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30002066 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10Note: Hover over icons to view figure legend
Alignments
SMED30002066 aligns in the following genomic locations:
Homology
BLAST of SMED30002066 vs. Planmine SMEST
Match: SMESG000016886.1 (SMESG000016886.1) HSP 1 Score: 625.165 bits (1611), Expect = 0.000e+0 Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 2 Query: 233 ISFYYFLGSILSAVAFSLEYDCQDRQCFISTNELNEKVFDCKPGVSDANLIQCSFVKHLKKITLTTKNESYIDIDKVSPGAFKRLTNGIEVNKIFINKLRMSSIGSADFDGLMNIAEMQIENPKILDKYTFYSLATTKTFFILTIICSPENNNNSFQDLLLKSSRWRLYSKACNRKRTCEIVNCESDKEATIYKITASNEVAKNDFQLISGICEKNKSCKNPATEKKSTPKPVEIEKVMPSQPVKSAKLNIVKKQRIDRYYDTLLWIVIILTGINSXXXXXXXXXXXXWWRKLSLSATNKKHTIVLEDDDTKYLKLTPYA 1192 ISFYYFLGSILSAVAFSLEYDCQDRQCFISTNELNEKVFDCKPGVSDANLIQCSFVKHLKKITLTTKNESYIDIDKVSPGAFKRLTNGIEVNKIFINKLRMSSIGSADFDGLMNIAEMQIENPKILDKYTFYSLATTKTFFILTIICSPENNNNSFQDLLLKSSRWRLYSKACNRKRTCEIVNCESDKEATIYKITASNEVAKNDFQLISGICEKNKSCKNPATEKKSTPKPVEIEKVMPSQPVKSAKLNIVKKQRIDRYYDTLLWIVIILTGINSFVILGILVFLIIWWRKLSLSATNKKHTIVLEDDDTKYLKLTPYA Sbjct: 3 ISFYYFLGSILSAVAFSLEYDCQDRQCFISTNELNEKVFDCKPGVSDANLIQCSFVKHLKKITLTTKNESYIDIDKVSPGAFKRLTNGIEVNKIFINKLRMSSIGSADFDGLMNIAEMQIENPKILDKYTFYSLATTKTFFILTIICSPENNNNSFQDLLLKSSRWRLYSKACNRKRTCEIVNCESDKEATIYKITASNEVAKNDFQLISGICEKNKSCKNPATEKKSTPKPVEIEKVMPSQPVKSAKLNIVKKQRIDRYYDTLLWIVIILTGINSFVILGILVFLIIWWRKLSLSATNKKHTIVLEDDDTKYLKLTPYA 322
BLAST of SMED30002066 vs. Planmine SMEST
Match: SMESG000016875.1 (SMESG000016875.1) HSP 1 Score: 625.165 bits (1611), Expect = 0.000e+0 Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 2 Query: 233 ISFYYFLGSILSAVAFSLEYDCQDRQCFISTNELNEKVFDCKPGVSDANLIQCSFVKHLKKITLTTKNESYIDIDKVSPGAFKRLTNGIEVNKIFINKLRMSSIGSADFDGLMNIAEMQIENPKILDKYTFYSLATTKTFFILTIICSPENNNNSFQDLLLKSSRWRLYSKACNRKRTCEIVNCESDKEATIYKITASNEVAKNDFQLISGICEKNKSCKNPATEKKSTPKPVEIEKVMPSQPVKSAKLNIVKKQRIDRYYDTLLWIVIILTGINSXXXXXXXXXXXXWWRKLSLSATNKKHTIVLEDDDTKYLKLTPYA 1192 ISFYYFLGSILSAVAFSLEYDCQDRQCFISTNELNEKVFDCKPGVSDANLIQCSFVKHLKKITLTTKNESYIDIDKVSPGAFKRLTNGIEVNKIFINKLRMSSIGSADFDGLMNIAEMQIENPKILDKYTFYSLATTKTFFILTIICSPENNNNSFQDLLLKSSRWRLYSKACNRKRTCEIVNCESDKEATIYKITASNEVAKNDFQLISGICEKNKSCKNPATEKKSTPKPVEIEKVMPSQPVKSAKLNIVKKQRIDRYYDTLLWIVIILTGINSFVILGILVFLIIWWRKLSLSATNKKHTIVLEDDDTKYLKLTPYA Sbjct: 3 ISFYYFLGSILSAVAFSLEYDCQDRQCFISTNELNEKVFDCKPGVSDANLIQCSFVKHLKKITLTTKNESYIDIDKVSPGAFKRLTNGIEVNKIFINKLRMSSIGSADFDGLMNIAEMQIENPKILDKYTFYSLATTKTFFILTIICSPENNNNSFQDLLLKSSRWRLYSKACNRKRTCEIVNCESDKEATIYKITASNEVAKNDFQLISGICEKNKSCKNPATEKKSTPKPVEIEKVMPSQPVKSAKLNIVKKQRIDRYYDTLLWIVIILTGINSFVILGILVFLIIWWRKLSLSATNKKHTIVLEDDDTKYLKLTPYA 322
BLAST of SMED30002066 vs. Planmine SMEST
Match: SMESG000005085.1 (SMESG000005085.1) HSP 1 Score: 167.162 bits (422), Expect = 3.895e-48 Identity = 105/305 (34.43%), Postives = 168/305 (55.08%), Query Frame = 2 Query: 251 LGSILSAVAFSLEYDCQDRQCFISTNELNEKVFDCKPGVSDANLIQCSFVKHLKKITLTTKNESYIDIDKVSPGAFKRLTNGIEVNKIFINKLRMSSIGSADFDGLMNIAEMQIENPKILDKYTFYSLATTKTFFILTIICSPENNNNSFQDLLLKSSRWRLYSKACNRKRTCEIVNCESDKEATIYKITASNEVAKNDFQLISGICEKNKSCKNPATEKK---STPKPVEIEKVMPSQPVKSAKLNIVKKQRIDRYYDTLLWIVIILTGINSXXXXXXXXXXXXWWRKLSLSATNKKHTIVLED 1156 L I+ +S C +R C + N NE +FDC G D +QC + K + +TL+T ++SY+ ID+++PGAF RL +GI + KIFINKL+ S+ +DF L N++ ++IENP L TF ++ + + F LTI C N N+SFQ L ++ WR++++ C+++ C C + I KI+ S FQL S C N SCK TP+ +++ + V S+ N K+++ D YYD L+ +++++ GINS IL I V LI+W R + S + + T+ + + Sbjct: 9 LTLIILICGYSNASKCSNRYCVTTLNANNETIFDCSLGQIDNTTVQCFYNKANQIMTLSTFDDSYMFIDQIAPGAFDRLFSGIVLEKIFINKLQFESLNGSDFMALSNVSSIKIENPIKLSFDTFLTVTSPRKIFYLTISCISGNKNSSFQVLHFRTPMWRIFTEDCHQRLACTHSTCNYARLDNIAKISPSKFYQAFQFQLSSHSCLLNSSCKTIQQANSVNIKTPQRFTVDE--KEKRVTSS--NTEKEEKKDNYYDFLMNVLLVIIGINSVFILIICVILIMWRRSIMASVSTRNRTLRIRN 309
BLAST of SMED30002066 vs. Planmine SMEST
Match: SMESG000035450.1 (SMESG000035450.1) HSP 1 Score: 72.0182 bits (175), Expect = 1.398e-13 Identity = 83/315 (26.35%), Postives = 149/315 (47.30%), Query Frame = 2 Query: 296 CQDRQCFISTNELNEKVFDCKPGVSDANLIQCSFVK---HLKKITLTTKNESYID--IDKVSPGAFKRLTNGI-EVNKIFINKLRMSSIGSADFDGLMNIAEMQIENPKILDKYTFYSLATTKTFFILTIICSPENNNNS-FQDLLLKSSRWRLYSKACNRKRTCEIVNCESD-KEATIY--KITASNEVAKNDFQLISGICEK-NKSCKNPATEKKSTPK-----PVEIEKVMPSQPVKSAKLNI-------VKKQRIDRYYDTLLWIVIILTGINSXXXXXXXXXXXXWWR---KLSLSATNKKHTIVLEDDD 1162 C+D CFI T +L+ +V C V AN F+K LK+ L K + D +D + G FK + + E+ ++ I +++++I +DF GL + + + NP L++ +F + ++ +F L I C+ E N++S L + S WR+ + C C + C + + Y K N+ +++ + S C K N C P T + K P+++ + S + S + +KK + Y + + +I++ I S +IL +L+ ++ WWR K + KHTIVL+DD+ Sbjct: 30 CEDIDCFIET-DLSARVLGCDYKVLLANKTNLKFLKCEYDLKRKALFIKPLTAGDNSVDGLLTGGFKVFSESLKEITELHIPHVKINNISRSDFVGLEKLRGLTLMNPGNLEEDSFLDIINSE-YFQLYIYCNNEKNHSSGISKLRISSKSWRIVLRDCRYSFGCNLGTCVNGVRIGNEYQVKFQLRNKSPESESVIRSSTCLKSNIHCVKPTTTMWPSSKSDMIIPLQVSTLEISSTISSKVRSYKGFTEEKIKKLKSAEY--SYGYKIILVLSIVSCMILIVLIIMVGWWRQRQKRNRKPCRGKHTIVLKDDN 340
BLAST of SMED30002066 vs. Planmine SMEST
Match: SMESG000035432.1 (SMESG000035432.1) HSP 1 Score: 54.6842 bits (130), Expect = 3.735e-8 Identity = 37/172 (21.51%), Postives = 81/172 (47.09%), Query Frame = 2 Query: 296 CQDRQCFISTNELNEKVFDCKPGVSDANLIQCSFVKHLKKITLTTKNESYIDIDKVSPGAFKRLTNGI-EVNKIFINKLRMSSIGSADFDGLMNIAEMQIENPKILDKYTFYSLATTKTFFILTIICSPENNNNSFQDLLLKSSRWRLYSKACNRKRTCEIVNCESDKEATI 808 C D +C++ + V + + +C + + K + L K+ +DK + G F+ + + ++ + IN +++ I +DF L N +++ P +D+ +F + +FF +++ C+ +++N F+ L+ +WR+Y CN + C +V E I Sbjct: 24 CGDLECYVDRISNTQYVNCSREHDPHSKTFECQYNEDEKSLKLKNKDHEVKSVDKFASGLFENIQKDLGKIKDLSINYIQVKYIKKSDFLTLFNARYLEMSIPMEIDEDSF--IPFNSSFFEISMKCNNDSSNLQFK---LQIEKWRIYLHYCNYRAACSLVRSGEKYEYNI 190 The following BLAST results are available for this feature:
BLAST of SMED30002066 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30002066 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30002066 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30002066 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30002066 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30002066 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30002066 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30002066 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30002066 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30002066 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30002066 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30002066 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30002066 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30002066 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30002066 ID=SMED30002066|Name=SMED30002066|organism=Schmidtea mediterranea sexual|type=transcript|length=1283bpback to top protein sequence of SMED30002066-orf-1 >SMED30002066-orf-1 ID=SMED30002066-orf-1|Name=SMED30002066-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=323bp MKISFYYFLGSILSAVAFSLEYDCQDRQCFISTNELNEKVFDCKPGVSDAback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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