Trans-aconitate 2-methyltransferase

Query:  181 ATYKKLLELGCGTGTITDFYIEYFKPESFLGIDEVEHYLEYARENHSKSSIEIKYEKCDLNNMEELKEILKKNLDVDLVISSFVLHWVEDPIELFKTISSNLNPGVEHFHISVVNFQEELIDLVEAYEGHPTWKVYYQDICKLPPLLSRVD-PIYPSKDFIQWILDQRFQIYLEQLVPVTNHFESVEMGKLFHRFLLPFLNFIPCHLREEFMKEFLSD 831
            A+ K ++++GCG G IT++     K  S +GID+    ++YA+E          Y   D   M+   E + K  D+  V+S F L W+ +    F  IS+ +  G + + ++ +  +   + L+        WK+++ +       L+ +   IY ++  I+    ++F++Y    +P T  F+  +    F+  +LP LN +    RE F+ E L+D
Sbjct:   44 ASNKTVIDIGCGNGKITNYISSLVKDGSVIGIDKDSSMIKYAKE---------TYPNVDFKVMDIQNENIDKKYDI--VVSFFCLPWIVNKQASFHHISNMMKSGSKLYILAAI-METNHVTLINNLMKKDHWKLFFVNYSSPFDYLNDIQYDIYANQSGIE---QKKFKVY---NIPYT--FKDRQSLHKFNLAILPQLNQLSIEHREIFITELLND 241          

Query:  184 TYKKLLELGCGTGTITDFYIEYFKPESFLGIDEVEHYLEYARENHSKSSIEIKYEKCDLNNMEELKEILKKNLDVDLVISSFVLHWVEDPIELFKTISSNLNPGVEHFHISVVNFQEEL-----IDLVEAYEGHPTWKVYYQDICKLPPLLSRVDPIYPSKDFIQWILDQRFQIYLEQLV---PVTNHFESVEMGKLFHRFLLPFLNFIPCHLREEFMKEFLSDSRKACRSVDD 861
            + ++ ++LGCG G  T+  IE F  E   G+D   + LE AR+    ++    + + DL + +  +         DL+ ++ V  W+ D +++F  +   L+PG     +  V   + L     + + E+    P WK  +++         R  P+ P   +   ++ +  +I +   +   P+ +  + VE  K     L+P+L       RE F+ ++L    KA   + D
Sbjct:   30 SLRRAVDLGCGPGNSTELIIERFGAEGVSGLDSDVNMLEAARKRLPGTA----FVEADLGSWQPTEP-------ADLLFANAVFQWLPDHLDIFDRLMDGLSPG----GVLAVQMPDNLGEASHLAMEESAHAGP-WKAAFEEKS------VRRQPLAPPSAYYSRLIGKAARIDIWHTIYNHPMADAADIVEWVK--GTGLMPYLAHAGEAYREAFLADYLERIEKAYPKMSD 239          

Query:   91 ALSGSEYELCGKNTKLYVSKMIQSYVMPNKATYKKLLELGCGTGTITDFYIEYFKPESFLGIDEVEHYLEYARENHSKSSIEIKYEKCDLNNMEELKEILKKNLDVDLVISSFVLHWVEDPIELFKTISSNLNPGV 498
            A+S  +Y L  K    ++S+M+    + N+   + +LE+GCGTG +T     YF    +  ++  +  LE A+ N  +  +   + + D       ++   K    DL++S     W   P    + ++  L PG 
Sbjct:   14 AVSYEKYALVQKKMADHLSQMVTE--ITNENDVRSILEIGCGTGGLTRVIRSYFSAAHYEAVEIAQGMLEQAKNNLEQHGLICSFSQADAEEWVWEQQAKSK----DLIVSGACFQWFARPAHTLRGLARILKPGA 143          

Query:   82 SFSALSGSEYELCGKNTKLYVSKMIQSYVMPNKATYKKLLELGCGTGTITDFYIEYF----KPESFL-GIDEVEHYLEYARENHSKSSI--EIKYEKCDLNNMEELKEILKKNLDVDLVISSFVLHWVEDPIELFKTISSNLNPGVEHFHISVVNFQEELIDLVEAYE 564
            SFS+ +  EY+   +  K  V  +++  +    +  + LL+LGCGTG   +  ++ F    +PE  +  +D  E  L+ A++   +  +  +I +   D+ ++  + E        D  ISS  + W E+P++LF  +   L PG   F +S +   E L +L  A+ 
Sbjct:  302 SFSS-AAQEYDTLARLQKRVVDSLVEFSLGTGGSVGQTLLDLGCGTGYCIERLLQQFPEITQPEGRIHALDIAEGMLDRAQQKFDELGVAEQINWHLGDMESLPFVDE------SFDGCISSLTVQWSENPLQLFSEMYRALKPG-GWFALSTLG-PETLFELRSAWR 460          

Query:  106 EYELCGKNTKLYVSKM--IQSYVMPNKATYKKLLELGCGTGTITDFYIEYFKPESFLGIDEVEHYLEYARENHSKSSIEIKYEKCDL-NNMEELKEILKKNLDVDLVISSFVLHWVEDPIELFKTISSNLNPGVEHFHISVVNFQEELIDLVEAYEGHPTWKVYYQDIC-KLPPLLSRVDPIYPSKDFIQWILDQRFQIYLEQLVPVTNHFESVEMGKLFHRFL------LPFLNFIPCHLREEFM 813
            +Y  C +  +   +K+  I + V+     +  ++++GCGTG +T  +++  K +  +  D+ E  +E+A EN+  S  EI Y+  D+ ++ ++LK  L      DLV S +  HWV D  + F  IS  L PG + F I +  +  +L      +  +  W  Y++++  K+  L      + P  D  +    Q  + +  +   +T   E V   K F+ F+       PF+ +IP   R+EFM
Sbjct:    7 QYNDCNELQRYEAAKLFSIITQVVGPSPKFGIVVDIGCGTGNVTLDFLQLVKCDQLIAFDKSEFMIEFATENNKSS--EINYKVADIRDDFQKLKFDLNIVERADLVFSVYCFHWVVDKSKAFHNISQLLKPGGKCFLIFI--YWSDLFAYQRQFVLNSKWSEYFENLDEKMSSLAPAYTAVLPDIDHTRKKWTQLLESFGFEKCKITVE-EDVHFFKEFNVFMYDFQSVCPFIYYIPTAKRDEFM 247          

Query:  151 MIQSYVMPNKATYKKLLELGCGTGTITDFYIEYFKPESFLGIDEVEHYLEYARENHSKSSIEIKYEKCDL-NNMEELKEILKKNLDVDLVISSFVLHWVEDPIELFKTISSNLNPGVEHFHISVVNFQEELIDLVEAYEGHPTWKVYYQDICKLPPLLSRVDPIYPSKDFIQWILDQRFQIYLE-QLVPVTNHFESVEMGKL----FHRFLL------PFLNFIPCHLREEFMKEFLSDSRK----ACRSVDD 861
            MIQ    P+ A +  ++++GCGTG +T  ++   + +  +  D+ +  +E+ARE     S E+ YE  D+ +N +ELK  +K +   DLV S + LHWV++  + F+ I+  L PG + + + +  +  +L      +     WK Y   I  +  +LS   P + +   +  I D   ++    ++   T    +++  K     F+ F+L      P++NF+P   R EF K++    RK      ++VDD
Sbjct:   25 MIQHDFGPS-AKFGTVVDIGCGTGNVTLDFLNSSQCDQLIAFDKNKSMIEFARE--INKSDEVNYEVADISDNFDELKYNIKLSKKADLVFSVYCLHWVQNKTKAFENINRFLKPGGKCYLLFM--YWSDLYMCDNEFILSSKWKKY---IPNIDEMLSTSAPFHKAS--LPNIADSTKKLKESMEIAGFTKCEVTIKQEKYFFEEFNHFMLDFHSVCPYVNFVPASERNEFFKDYFDFLRKNYNTGSKNVDD 267          

Query:  196 LLELGCGTGTITDFYIEYFKPESFLGIDEVEHYLEYARENHSKSSIEIKYEKCDLNNMEELKEILKKNLDVDLVISSFVLHWVEDPIELFKTISSNLNP-GVEHFHISVVNFQEELIDLVEAYEGHPTWKVYYQDICKLPPLLSRVDPIYPSKDFIQWILDQRFQIYLEQLVPVTNHFESVEMGKLFHRFL-LPFLNFIPCHLREEFM 813
            LL++GCG G IT  +       S LGID  +  +E AR    K+   ++Y   D   M+     +  N   D++ S+ VLHW+ D   + K++ ++L P G   F +       EL+  ++     P WK Y+ D    P         Y  +++ +W+ +  F+    QL+P     +  E  K + R    P+ N IP  LRE F+
Sbjct:   38 LLDIGCGDGKITAEFASILNKGSVLGIDSSKTMVELAR----KTFPGVEYPNLDFKLMDATA--INLNHGFDVIFSNAVLHWINDHGSVLKSVRNHLKPHGKLLFQMGGKGNARELLLFIDEIITAPDWKEYFIDF-NFPY------SFYGIEEYQRWLPENGFKAQRVQLIPKDMQHQGKERLKGWLRTTWFPYTNRIPQKLRETFL 232          

Query:  190 KKLLELGCGTGTITDFYIEYFKPESFLGIDEVEHYLEYARENHSKSSIEIKYEK-CDLNNMEELKEILKKNLDVDLVISSFVLHWVEDPIELFKTISSNLNPGVEHFHISVVNFQEELIDLVEAYEGHPTWKVYYQ----------DICKLPPLLSRVD----PIYPSKD----FIQW--ILD----QRFQIYLEQLVPVTNHFESV--EMGKLFHRFLLPFLNFIPCHLREEFMKEFLSDSRKACRSVDDKV 867
            K ++++GCGTG +T   + +   +  +  D+ +  +E+AR+ H  + I  +    CD     +LK  +  N +VD+V S   LHW+ D  +  + IS  L PG + +  +++ F  +++ L E     P W  Y+           D C  P    R      P+Y   D      +W  + D       Q+ +++ V   + F     E+  L H     FL +IP  L++EF+ ++  D  K C + D K+
Sbjct:   36 KSVIDVGCGTGNVTADILRFTDCDKLIAFDKSDSMIEFARQRHRNAKISFQIADICD--EWSKLKSDINLNEEVDVVFSVHCLHWITDKAKAIENISKMLKPGGQCY--ALLFFWSDILPLQEQITHFPKWNKYFSHLEKSDVTTTDKCLSPGKQRRRSSAPFPVYLRSDEQATINEWKSLCDSSGFSETQVSVKKSVFCFDDFTGFKDEVTSLCH-----FLRYIPDTLKQEFLNDYF-DHVKNCYTSDQKI 278          

Query:  196 LLELGCGTGTITDFYIEYFKPESFLGIDEVEHYLEYARENHSKSSIEIKYEKCDLNNMEELKEILKKNLDVDLVISSFVLHWVEDPIELFKTISSNLNP-GVEHFHISVVNFQEELIDLVEAYEGHPTWKVYYQDICKLPPLLSRVDPIYPSKDFIQWILDQRFQIYLEQLVPVTNHFESVEMGKLFHRFL-LPFLNFIPCHLREEFM 813
            LL++GCG G IT  +    K  S LGID  +  +E AR    K+   +KY   +   M+     +  N   D+V S+ VLHW+ D   + K + ++L P G   F +       EL+ ++E    +  WK Y+ D    P         Y  +D+  W+ +  F+    QL+P     +  E  K + R    P+ N +P  L+E F+
Sbjct:   38 LLDIGCGDGKITAEFASIIKNGSVLGIDSSQTMVELAR----KTFPAVKYPNLEFKFMDATS--INLNHSFDVVFSNAVLHWINDHGSVLKGVRNHLKPHGKLLFQMGGKGNARELLLIIEEVITYTDWKEYFIDFT-FPY------SFYGIEDYQTWLPENGFKAERLQLIPKDMQHQGKEGLKGWLRTTWFPYTNRLPQELKETFL 232          

Query:  178 KATYKKLLELGCGTGTITDFYIEYFKPESFLGIDEVEHYLEYARENHSKSSIEIKYEKCDLNNMEELKEILKKNLDVDLVISSFVLHWVEDPIELFKTISSNL-NPGVEHFHISVVNFQEELIDLVEA 558
            K T K L+++GCGTG  T     YFK    +GID     L  A +  ++  ++ K    +    E+L  I  ++  VD+VIS+  +HW  +   LF+ +SS L + G   F   +   Q E +D  EA
Sbjct:   36 KGTRKSLVDIGCGTGKATFVVEPYFK--EVIGIDPSSAMLSIAEKETNERRLDKKIRFINAPG-EDLSSIRPES--VDMVISAEAIHWC-NLERLFQQVSSILRSDGTFAFWFYI---QPEFVDFPEA 154          

Query:  181 ATYKKLLELGCGTGTITDFYIEYFKPE-SFLGIDEVEHYLEYARENHSKSSIEIKYEKCDLNNMEELKEILKKNLDVDLVISSFVLHWVEDPIELFKTISSNLNPG 495
            AT K +L+LGCGTG +T      F  + S +GID  ++ +E A+ NH  +   +++ +    +   L E        DLV S+FVLHW+    + F+ I  +L PG
Sbjct:   34 ATAKFVLDLGCGTGDVTGAMAANFSNDASIIGIDPDKYRVELAKSNHCSN---VQFLQGSAESFPHLGEEY-----YDLVFSNFVLHWIPQRTKAFRDIYDSLKPG 131          

Query:  181 ATYKKLLELGCGTGTITDFYIEYFKPE-SFLGIDEVEHYLEYARENHSKSSIEIKYEKCDLNNMEELKEILKKNLDVDLVISSFVLHWVEDPIELFKTISSNLNPG 495
            AT K +L+LGCGTG +T      F  + S +GID  ++ +E A+ NH  +   +++ +    +   L E        DLV S+FVLHW+    + F+ I  +L PG
Sbjct:   34 ATAKFVLDLGCGTGDVTGAMAANFSNDASIIGIDPDKYRVELAKSNHCSN---VQFLQGSAESFPHLGEEY-----YDLVFSNFVLHWIPQRTKAFRDIYDSLKPG 131          

Query:   79 MSFSALSGSEYELCGKNTKLYVSKMIQSYVMPNKATYKKLLELGCGTGTITDFYIEYFKPESFLGIDEVEHYLEYARENHSKSSIEIKYEKCDLNNMEELKEILKKNLDVDLVISSFVLHWVEDPIELFKTISSNLNPGVEHFHISVVNFQEELIDLVEAYEGHPTWKVYYQDICKLPPLLSRVDPIYPSKDFIQWILDQRFQIYLEQLVPVTNHFESVEMGKLFHRFLLPFLNFIPCHLREEFMKEFLSDSRKACRSVDDKVTWKYNLFLFHTKN 906
            MSFSALSGSEYELCGKNTKLYVSKMIQSYVMPNKATYKKLLELGCGTGTITDFYIEYFKPESFLGIDEVEHYLEYARENHSKSSIEIKYEKCDLNNMEELKEILKKNLDVDLVISSFVLHW+EDPIELFKTISSNLNPGVEHFHISVVNFQEELIDLVEAYEGHPTWKVYYQDICKLPPLLSRVDPIYPSKDFIQWILDQRFQIYLEQLVPVTNHFESVEMG+LFH+FLLPFLNFIPCHLREEFMKEFLSDSRKACRSVDDKVTWKYNLFLFHTKN
Sbjct:    1 MSFSALSGSEYELCGKNTKLYVSKMIQSYVMPNKATYKKLLELGCGTGTITDFYIEYFKPESFLGIDEVEHYLEYARENHSKSSIEIKYEKCDLNNMEELKEILKKNLDVDLVISSFVLHWLEDPIELFKTISSNLNPGVEHFHISVVNFQEELIDLVEAYEGHPTWKVYYQDICKLPPLLSRVDPIYPSKDFIQWILDQRFQIYLEQLVPVTNHFESVEMGELFHKFLLPFLNFIPCHLREEFMKEFLSDSRKACRSVDDKVTWKYNLFLFHTKN 276          

Query:   79 MSFSALSGSEYELCGKNTKLYVSKMIQSYVMPNKATYKKLLELGCGTGTITDFYIEYFKPESFLGIDEVEHYLEYARENHSKSSIEIKYEKCDLNNMEELKEILKKNLDVDLVISSFVLHWVEDPIELFKTISSNLNPGVEHFHISVVNFQEELIDLVEAYEGHPTWKVYYQDICKLPPLLSRVDPIYPSKDFIQWILDQRFQIYLEQLVPVTNHFESVEMGKLFHRFLLPFLNFIPCHLREEFMKEFLSDSRKACRSVDDKVTWKYNLFLFHTKN 906
            MSF AL+  +Y+  G+ T+ +V  M+  +V+P K TYKK+L+LGCGTG ITDF+IE+FKPESF+GID  E +L + R+    S+IEIKYEKC+LN+MEELKEILK + D+DLV+SSFVLHW+ DPI LF+T+S  LN  VEH HISV N   +LIDL+  YE HP WK YY+D C++PPL +R++PI+ SK+F + ILD +F+IYL+  +P+ + F + E+  +F +F+LPF   IP HL +EF+ EF+++S    R+ + +  W Y+ F+ H KN
Sbjct:    1 MSFLALTEEDYDKYGQITQSFVKGMMTKFVIPKKKTYKKMLDLGCGTGNITDFFIEFFKPESFIGIDTNESFLNFGRQRPYISTIEIKYEKCNLNDMEELKEILKNHPDIDLVVSSFVLHWINDPITLFQTLSLCLNGHVEHAHISVRNIPIDLIDLIGEYENHPKWKTYYEDKCQIPPLFNRIEPIFSSKEFYEKILDHKFEIYLQTSIPICHQFVTDELFTIFIKFILPFTKHIPKHLHDEFINEFVNNSSGFYRNDNGRYAWDYDFFVCHAKN 276          

Query:   79 MSFSALSGSEYELCGKNTKLYVSKMIQSYVMPNKATYKKLLELGCGTGTITDFYIEYFKPESFLGIDEVEHYLEYARENHSKSSIEIKYEKCDLNNMEELKEILKKNLDVDLVISSFVLHWVEDPIELFKTISSNLNPGVEHFHISVVNFQEELIDLVEAYEGHPTWKVYYQDICKLPPLLSRVDPIYPSKDFIQWILDQRFQIYLEQLVPVTNHFESVEMGKLFHRFLLPFLNFIPCHLREEFMKEFLSDSRKACRSVDDKVTWKYNLFLFHTK 903
            M F A++G EY+             I+  V+P K +Y KLL++G GTG+ TDF ++++ P+SFLG+D+ E +LEYAR+   KSS+EI YEKCD  + ++LK++L+K+ D+DLV+SS  +H++ DPI+LF+T  S L   VEH H+SV    +ELID VE Y  H  WK+Y++   ++ P+ S  + +YPS+  ++ +LD  ++I+LE+  PV+N F S E  K  +R LLP +  IP HL+ EF+ EF+++SR  CR+ ++KV W+Y+LF+ H K
Sbjct:    1 MHFFAINGDEYDEFMGYVNKTSKNAIKYNVVPLKKSYNKLLDIGSGTGSFTDFLVDFYHPKSFLGLDQSEGFLEYARQRTYKSSVEINYEKCDFGDPKQLKKVLRKHPDIDLVVSSHSIHFLNDPIKLFETFISCLTGDVEHVHVSVRKLSQELIDFVENYRNHQEWKIYFEKDYQVSPMFSSDETVYPSEKSLRQLLDLNYKIHLEKFFPVSNEFYSYEQLKYVYRSLLPLMQIIPDHLKNEFIDEFMAESRNLCRNTENKVFWQYDLFILHAK 275