POU domain protein

Overview


Name	POU domain protein
Smed ID	SMED30002016
Length (bp)	1339

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of POU domain protein (SMED30002016) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for POU domain protein (SMED30002016) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30002016

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
nervous system	SMED30002016	SMESG000030759.1	dd_Smed_v4_30562_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30002016	SMESG000030759.1	dd_Smed_v4_30562_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30002016	SMESG000030759.1	dd_Smed_v4_30562_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of POU domain protein vs. Ensembl Human
Match: POU4F3 (POU class 4 homeobox 3 [Source:HGNC Symbol;Acc:HGNC:9220])

HSP 1 Score: 251.906 bits (642), Expect = 1.070e-79
Identity = 122/160 (76.25%), Postives = 135/160 (84.38%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTL 1086
            D+E + RELEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKPVLQ WLE+AE   +E+ +  + F+   ERKRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S +
Sbjct:  179 DVESDPRELEAFAERFKQRRIKLGVTQADVGAALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPVLQAWLEEAEAAYREKNSKPELFNG-SERKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAV 337

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BLAST of POU domain protein vs. Ensembl Human
Match: POU4F2 (POU class 4 homeobox 2 [Source:HGNC Symbol;Acc:HGNC:9219])

HSP 1 Score: 249.595 bits (636), Expect = 5.027e-78
Identity = 119/159 (74.84%), Postives = 134/159 (84.28%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            D++ + R+LEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE   +E+L   + F+   E+KRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S 
Sbjct:  250 DVDADPRDLEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKSHREKLTKPELFNGA-EKKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSA 407

[Close]

BLAST of POU domain protein vs. Ensembl Human
Match: POU4F1 (POU class 4 homeobox 1 [Source:HGNC Symbol;Acc:HGNC:9218])

HSP 1 Score: 248.825 bits (634), Expect = 1.350e-77
Identity = 120/159 (75.47%), Postives = 134/159 (84.28%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            D + + RELEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE   +E++N  + F+   E+KRKRTSI   EKRSLEA+F +QPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMKFS 
Sbjct:  260 DSDTDPRELEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEGAQREKMNKPELFNG-GEKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFSA 417

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BLAST of POU domain protein vs. Ensembl Human
Match: POU3F2 (POU class 3 homeobox 2 [Source:HGNC Symbol;Acc:HGNC:9215])

HSP 1 Score: 147.132 bits (370), Expect = 8.848e-39
Identity = 77/149 (51.68%), Postives = 104/149 (69.80%), Query Frame = 1

Query:  628 ELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSL-SQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            +LE FA+ FKQRRIKL  TQADVG ALG L     G++ SQ+TICRFE+L LS  NM  LKP+L  WLE+A++ +    +  D  +    +++KRTSI  S K +LE+ F   P+PS+++I  +A+ L L+K VVRVWFCN+RQK+KRM
Sbjct:  269 DLEQFAKQFKQRRIKLGFTQADVGLALGTL----YGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEEADSSSGSPTS-IDKIAAQGRKRKKRTSIEVSVKGALESHFLKCPKPSAQEITSLADSLQLEKEVVRVWFCNRRQKEKRM 412

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BLAST of POU domain protein vs. Ensembl Human
Match: POU3F4 (POU class 3 homeobox 4 [Source:HGNC Symbol;Acc:HGNC:9217])

HSP 1 Score: 143.665 bits (361), Expect = 3.076e-38
Identity = 77/149 (51.68%), Postives = 101/149 (67.79%), Query Frame = 1

Query:  628 ELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSL-SQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            ELE FA+ FKQRRIKL  TQADVG ALG L     G++ SQ+TICRFE+L LS  NM  LKP+L  WLE+A++      +  D  +    +++KRTSI  S K  LE  F   P+P++++I  +A+ L L+K VVRVWFCN+RQK+KRM
Sbjct:  193 ELEQFAKQFKQRRIKLGFTQADVGLALGTL----YGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEEADSSTGSPTS-IDKIAAQGRKRKKRTSIEVSVKGVLETHFLKCPKPAAQEISSLADSLQLEKEVVRVWFCNRRQKEKRM 336

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BLAST of POU domain protein vs. Ensembl Celegans
Match: unc-86 (Transcription factor unc-86 [Source:UniProtKB/Swiss-Prot;Acc:P13528])

HSP 1 Score: 218.394 bits (555), Expect = 9.368e-68
Identity = 106/160 (66.25%), Postives = 125/160 (78.12%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDID------ERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKR 1068
            D++ + R+LE FAE FKQRRIKL VTQADVG+AL +LK+ GVGSLSQSTICRFESLTLSHNNM+ALKP+L +WLE AE   K++    D   DI+      ++KRKRTSI   EKR LE FF  QPRPS E+I  IA++L+LKKNVVRVWFCNQRQKQKR
Sbjct:   99 DMDTDPRQLETFAEHFKQRRIKLGVTQADVGKALAHLKMPGVGSLSQSTICRFESLTLSHNNMVALKPILHSWLEKAEEAMKQK----DTIGDINGILPNTDKKRKRTSIAAPEKRELEQFFKQQPRPSGERIASIADRLDLKKNVVRVWFCNQRQKQKR 254

[Close]

BLAST of POU domain protein vs. Ensembl Celegans
Match: unc-86 (Transcription factor unc-86 [Source:UniProtKB/Swiss-Prot;Acc:P13528])

HSP 1 Score: 218.779 bits (556), Expect = 3.464e-67
Identity = 106/160 (66.25%), Postives = 125/160 (78.12%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDID------ERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKR 1068
            D++ + R+LE FAE FKQRRIKL VTQADVG+AL +LK+ GVGSLSQSTICRFESLTLSHNNM+ALKP+L +WLE AE   K++    D   DI+      ++KRKRTSI   EKR LE FF  QPRPS E+I  IA++L+LKKNVVRVWFCNQRQKQKR
Sbjct:  155 DMDTDPRQLETFAEHFKQRRIKLGVTQADVGKALAHLKMPGVGSLSQSTICRFESLTLSHNNMVALKPILHSWLEKAEEAMKQK----DTIGDINGILPNTDKKRKRTSIAAPEKRELEQFFKQQPRPSGERIASIADRLDLKKNVVRVWFCNQRQKQKR 310

[Close]

BLAST of POU domain protein vs. Ensembl Celegans
Match: ceh-6 (Homeobox protein ceh-6 [Source:UniProtKB/Swiss-Prot;Acc:P20268])

HSP 1 Score: 135.961 bits (341), Expect = 1.262e-35
Identity = 78/167 (46.71%), Postives = 107/167 (64.07%), Query Frame = 1

Query:  577 QQPIQAVNQGDIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSL-SQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRK-RTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
             QP+  ++    +    +LE FA+ FKQRRIKL  TQADVG ALG L     G++ SQ+TICRFE+L LS  NM  LKP+L  WLE+A++      +  +  +    RKRK RTSI  + K  LE  F    +P++++I Q+A +L L+K VVRVWFCN+RQK+KR+
Sbjct:  177 HQPLSDISDDSEQTCPDDLEGFAKQFKQRRIKLGYTQADVGVALGTL----YGNIFSQTTICRFEALQLSFKNMCKLKPLLFKWLEEADSTTGSPNSTFEKMTGQAGRKRKKRTSIEVNVKSRLEFHFQSNQKPNAQEITQVAMELQLEKEVVRVWFCNRRQKEKRI 339

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BLAST of POU domain protein vs. Ensembl Celegans
Match: ceh-18 (Homeobox protein ceh-18 [Source:UniProtKB/Swiss-Prot;Acc:P41934])

HSP 1 Score: 125.946 bits (315), Expect = 3.610e-31
Identity = 74/194 (38.14%), Postives = 101/194 (52.06%), Query Frame = 1

Query:  604 GDIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFS---------------DIDE-----------------------RKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            GD   +  ELEAFA+ FK++RIK   TQ DVG ALG  K  G    SQ+TI RFE+L LS  NM  L+P+L+ WL D E   +     +D                  DI E                       R+RKRT++  +++ +L+ FF + PRP  +K+  IA  L L ++VVRVWFCN+RQK +R+
Sbjct:  287 GDDRIDMNELEAFAQTFKKQRIKFGFTQGDVGVALG--KRYGT-DFSQTTISRFEALNLSFKNMCKLRPLLKEWLADVEMAIEGGATVTDLIDKKTIHNGNHHTIHHVDIHETSISNSISSVTASSLLSREQHVKRRRKRTNLDMNQRNALDTFFALNPRPDHDKMTDIANSLELDRDVVRVWFCNRRQKMRRV 477

[Close]

BLAST of POU domain protein vs. Ensembl Celegans
Match: ceh-18 (Homeobox protein ceh-18 [Source:UniProtKB/Swiss-Prot;Acc:P41934])

HSP 1 Score: 125.946 bits (315), Expect = 3.739e-31
Identity = 74/194 (38.14%), Postives = 101/194 (52.06%), Query Frame = 1

Query:  604 GDIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFS---------------DIDE-----------------------RKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            GD   +  ELEAFA+ FK++RIK   TQ DVG ALG  K  G    SQ+TI RFE+L LS  NM  L+P+L+ WL D E   +     +D                  DI E                       R+RKRT++  +++ +L+ FF + PRP  +K+  IA  L L ++VVRVWFCN+RQK +R+
Sbjct:  289 GDDRIDMNELEAFAQTFKKQRIKFGFTQGDVGVALG--KRYGT-DFSQTTISRFEALNLSFKNMCKLRPLLKEWLADVEMAIEGGATVTDLIDKKTIHNGNHHTIHHVDIHETSISNSISSVTASSLLSREQHVKRRRKRTNLDMNQRNALDTFFALNPRPDHDKMTDIANSLELDRDVVRVWFCNRRQKMRRV 479

[Close]

BLAST of POU domain protein vs. Ensembl Fly
Match: acj6 (gene:FBgn0000028 transcript:FBtr0305192)

HSP 1 Score: 244.202 bits (622), Expect = 8.938e-77
Identity = 117/157 (74.52%), Postives = 131/157 (83.44%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDI--DERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            D + + RELEAFAE FKQRRIKL VTQADVG+AL NLKL GVG+LSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE +AK +    DA S +   E+KRKRTSI   EKRSLEA+F +QPRPS EKI  IAEKL+LKKNVVRVWFCNQRQKQKR+
Sbjct:  185 DTDTDPRELEAFAERFKQRRIKLGVTQADVGKALANLKLPGVGALSQSTICRFESLTLSHNNMIALKPILQAWLEEAEAQAKNKRRDPDAPSVLPAGEKKRKRTSIAAPEKRSLEAYFAVQPRPSGEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRI 341

[Close]

BLAST of POU domain protein vs. Ensembl Fly
Match: acj6 (gene:FBgn0000028 transcript:FBtr0305191)

HSP 1 Score: 244.202 bits (622), Expect = 1.041e-76
Identity = 117/157 (74.52%), Postives = 131/157 (83.44%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDI--DERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            D + + RELEAFAE FKQRRIKL VTQADVG+AL NLKL GVG+LSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE +AK +    DA S +   E+KRKRTSI   EKRSLEA+F +QPRPS EKI  IAEKL+LKKNVVRVWFCNQRQKQKR+
Sbjct:  192 DTDTDPRELEAFAERFKQRRIKLGVTQADVGKALANLKLPGVGALSQSTICRFESLTLSHNNMIALKPILQAWLEEAEAQAKNKRRDPDAPSVLPAGEKKRKRTSIAAPEKRSLEAYFAVQPRPSGEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRI 348

[Close]

BLAST of POU domain protein vs. Ensembl Fly
Match: acj6 (gene:FBgn0000028 transcript:FBtr0305193)

HSP 1 Score: 243.817 bits (621), Expect = 1.303e-76
Identity = 117/157 (74.52%), Postives = 131/157 (83.44%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDI--DERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            D + + RELEAFAE FKQRRIKL VTQADVG+AL NLKL GVG+LSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE +AK +    DA S +   E+KRKRTSI   EKRSLEA+F +QPRPS EKI  IAEKL+LKKNVVRVWFCNQRQKQKR+
Sbjct:  196 DTDTDPRELEAFAERFKQRRIKLGVTQADVGKALANLKLPGVGALSQSTICRFESLTLSHNNMIALKPILQAWLEEAEAQAKNKRRDPDAPSVLPAGEKKRKRTSIAAPEKRSLEAYFAVQPRPSGEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRI 352

[Close]

BLAST of POU domain protein vs. Ensembl Fly
Match: acj6 (gene:FBgn0000028 transcript:FBtr0305190)

HSP 1 Score: 242.276 bits (617), Expect = 4.397e-76
Identity = 114/155 (73.55%), Postives = 129/155 (83.23%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            D + + RELEAFAE FKQRRIKL VTQADVG+AL NLKL GVG+LSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE +AK +    DA S +   ++KRTSI   EKRSLEA+F +QPRPS EKI  IAEKL+LKKNVVRVWFCNQRQKQKR+
Sbjct:  185 DTDTDPRELEAFAERFKQRRIKLGVTQADVGKALANLKLPGVGALSQSTICRFESLTLSHNNMIALKPILQAWLEEAEAQAKNKRRDPDAPSVLPAGEKKRTSIAAPEKRSLEAYFAVQPRPSGEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRI 339

[Close]

BLAST of POU domain protein vs. Ensembl Fly
Match: acj6 (gene:FBgn0000028 transcript:FBtr0305196)

HSP 1 Score: 243.047 bits (619), Expect = 4.453e-76
Identity = 117/157 (74.52%), Postives = 131/157 (83.44%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDI--DERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            D + + RELEAFAE FKQRRIKL VTQADVG+AL NLKL GVG+LSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE +AK +    DA S +   E+KRKRTSI   EKRSLEA+F +QPRPS EKI  IAEKL+LKKNVVRVWFCNQRQKQKR+
Sbjct:  204 DTDTDPRELEAFAERFKQRRIKLGVTQADVGKALANLKLPGVGALSQSTICRFESLTLSHNNMIALKPILQAWLEEAEAQAKNKRRDPDAPSVLPAGEKKRKRTSIAAPEKRSLEAYFAVQPRPSGEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRI 360

[Close]

BLAST of POU domain protein vs. Ensembl Zebrafish
Match: pou4f3 (POU class 4 homeobox 3 [Source:NCBI gene;Acc:30534])

HSP 1 Score: 252.292 bits (643), Expect = 3.422e-80
Identity = 131/207 (63.29%), Postives = 148/207 (71.50%), Query Frame = 1

Query:  484 GSNCPPNVNIXXXXXXXXXXXXXXXXXXXXMQQP-IQAVNQG-----DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTL 1086
            G   PP+ ++      Q             M  P   +V+ G     D+E + RELEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKPVLQ WLE+AE   +E+ NG     + +ERKRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S +
Sbjct:  125 GMGAPPDPSVMTTQAHQHHLQMGHLHQAMAMGHPHTLSVHNGMACVNDVESDPRELEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPVLQAWLEEAEAAYREK-NGKPDLFNGNERKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAV 330

[Close]

BLAST of POU domain protein vs. Ensembl Zebrafish
Match: pou4f1 (POU class 4 homeobox 1 [Source:ZFIN;Acc:ZDB-GENE-000523-2])

HSP 1 Score: 248.825 bits (634), Expect = 2.566e-78
Identity = 119/159 (74.84%), Postives = 134/159 (84.28%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            D + + RELEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE   +E+++  D F+   E+KRKRTSI   EKRSLEA+F +QPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKR+KFS 
Sbjct:  209 DSDTDPRELEAFAERFKQRRIKLGVTQADVGGALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEGAQREKMSKPDIFNG-SEKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRLKFSA 366

[Close]

BLAST of POU domain protein vs. Ensembl Zebrafish
Match: pou4f2 (POU class 4 homeobox 2 [Source:ZFIN;Acc:ZDB-GENE-040401-1])

HSP 1 Score: 246.899 bits (629), Expect = 2.811e-78
Identity = 119/159 (74.84%), Postives = 135/159 (84.91%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            D++ + R+LEAFAE FKQRRIKL VTQADVG AL +LK+ GVGSLSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE   +E+LN  + F+   E+KRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S 
Sbjct:  160 DVDADPRDLEAFAERFKQRRIKLGVTQADVGSALASLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKSHREKLNKPELFNGA-EKKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSA 317

[Close]

BLAST of POU domain protein vs. Ensembl Zebrafish
Match: pou4f2 (POU class 4 homeobox 2 [Source:ZFIN;Acc:ZDB-GENE-040401-1])

HSP 1 Score: 248.054 bits (632), Expect = 6.029e-78
Identity = 119/159 (74.84%), Postives = 135/159 (84.91%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            D++ + R+LEAFAE FKQRRIKL VTQADVG AL +LK+ GVGSLSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE   +E+LN  + F+   E+KRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S 
Sbjct:  225 DVDADPRDLEAFAERFKQRRIKLGVTQADVGSALASLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKSHREKLNKPELFNGA-EKKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSA 382

[Close]

BLAST of POU domain protein vs. Ensembl Zebrafish
Match: zgc:158291 (zgc:158291 [Source:ZFIN;Acc:ZDB-GENE-061215-70])

HSP 1 Score: 244.588 bits (623), Expect = 5.458e-77
Identity = 117/160 (73.12%), Postives = 133/160 (83.12%), Query Frame = 1

Query:  604 GDIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            GD E + RELE+FAE FKQRRIKL VTQADVG AL NLK+ GVG LSQSTICRFESLTLSHNNM+ALKP+L+ WLE+AE   +E++   + F+  D +KRKRTSI   EKRSLEA+F +QPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMKFS 
Sbjct:  183 GDSEPDPRELESFAERFKQRRIKLGVTQADVGSALANLKIPGVGCLSQSTICRFESLTLSHNNMVALKPILEAWLEEAERAQREKMAKPEIFNGGD-KKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFSA 341

[Close]

BLAST of POU domain protein vs. Ensembl Xenopus
Match: POU4F1 (POU class 4 homeobox 1 [Source:NCBI gene;Acc:100488759])

HSP 1 Score: 252.292 bits (643), Expect = 1.448e-79
Identity = 122/159 (76.73%), Postives = 134/159 (84.28%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            D E + RELEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE   +E+LN  + F+   E+KRKRTSI   EKRSLEA+F +QPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMKFS 
Sbjct:  212 DSETDPRELEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEGAQREKLNKPELFNG-GEKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFSA 369

[Close]

BLAST of POU domain protein vs. Ensembl Xenopus
Match: rnf220.1 (ring finger protein 220, gene 1 [Source:Xenbase;Acc:XB-GENE-485089])

HSP 1 Score: 250.366 bits (638), Expect = 3.384e-79
Identity = 121/160 (75.62%), Postives = 134/160 (83.75%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTL 1086
            D+E + RELEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKPVLQ WL++AE   +E+ N      +  ERKRKRTSI   EKRSLEA+F+IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S +
Sbjct:  178 DVESDPRELEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPVLQAWLDEAEAAYREK-NAKPELFNSSERKRKRTSIAAPEKRSLEAYFSIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAV 336

[Close]

BLAST of POU domain protein vs. Ensembl Xenopus
Match: ppp2r2a (protein phosphatase 2 regulatory subunit B, alpha [Source:Xenbase;Acc:XB-GENE-971147])

HSP 1 Score: 247.669 bits (631), Expect = 1.312e-77
Identity = 119/159 (74.84%), Postives = 134/159 (84.28%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            D++ + R+LEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE   +E+L   + F+   E+KRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S 
Sbjct:  220 DVDADPRDLEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKSHREKLTKPELFNGA-EKKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSA 377

[Close]

BLAST of POU domain protein vs. Ensembl Xenopus
Match: pou4f1.2 (POU class 4 homeobox 1 [Source:NCBI gene;Acc:100037865])

HSP 1 Score: 242.662 bits (618), Expect = 3.111e-75
Identity = 115/156 (73.72%), Postives = 131/156 (83.97%), Query Frame = 1

Query:  613 EYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFS 1080
            E + RELE+FAE FKQRRIKL VTQADVG AL NLK+ GVG LSQSTICRFESLTLSHNNM+ALKP+L+ WLE+AE   +E++   + F+  D +KRKRTSI   EKRSLEA+F +QPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMKFS
Sbjct:  255 ETDPRELESFAERFKQRRIKLGVTQADVGSALANLKIPGVGCLSQSTICRFESLTLSHNNMVALKPILEAWLEEAERAQREKMAKPEIFTGGD-KKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFS 409

[Close]

BLAST of POU domain protein vs. Ensembl Xenopus
Match: pou3f2 (POU class 3 homeobox 2 [Source:NCBI gene;Acc:100486226])

HSP 1 Score: 147.132 bits (370), Expect = 2.270e-39
Identity = 77/149 (51.68%), Postives = 104/149 (69.80%), Query Frame = 1

Query:  628 ELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSL-SQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            +LE FA+ FKQRRIKL  TQADVG ALG L     G++ SQ+TICRFE+L LS  NM  LKP+L  WLE+A++ +    +  D  +    +++KRTSI  S K +LE+ F   P+PS+++I  +A+ L L+K VVRVWFCN+RQK+KRM
Sbjct:  208 DLEQFAKQFKQRRIKLGFTQADVGLALGTL----YGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEEADSSSGSPTS-IDKIAAQGRKRKKRTSIEVSVKGALESHFLKCPKPSAQEITSLADSLQLEKEVVRVWFCNRRQKEKRM 351

[Close]

BLAST of POU domain protein vs. Ensembl Mouse
Match: Pou4f3 (POU domain, class 4, transcription factor 3 [Source:MGI Symbol;Acc:MGI:102523])

HSP 1 Score: 251.521 bits (641), Expect = 7.626e-81
Identity = 122/160 (76.25%), Postives = 135/160 (84.38%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTL 1086
            D+E + RELEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKPVLQ WLE+AE   +E+ +  + F+   ERKRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S +
Sbjct:   97 DVESDPRELEAFAERFKQRRIKLGVTQADVGAALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPVLQAWLEEAEAAYREKNSKPELFNG-SERKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAV 255

[Close]

BLAST of POU domain protein vs. Ensembl Mouse
Match: Pou4f3 (POU domain, class 4, transcription factor 3 [Source:MGI Symbol;Acc:MGI:102523])

HSP 1 Score: 251.521 bits (641), Expect = 7.670e-80
Identity = 122/160 (76.25%), Postives = 135/160 (84.38%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTL 1086
            D+E + RELEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKPVLQ WLE+AE   +E+ +  + F+   ERKRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S +
Sbjct:  179 DVESDPRELEAFAERFKQRRIKLGVTQADVGAALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPVLQAWLEEAEAAYREKNSKPELFNG-SERKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAV 337

[Close]

BLAST of POU domain protein vs. Ensembl Mouse
Match: Pou4f2 (POU domain, class 4, transcription factor 2 [Source:MGI Symbol;Acc:MGI:102524])

HSP 1 Score: 249.21 bits (635), Expect = 5.486e-78
Identity = 119/159 (74.84%), Postives = 134/159 (84.28%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            D++ + R+LEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE   +E+L   + F+   E+KRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S 
Sbjct:  252 DVDADPRDLEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKSHREKLTKPELFNGA-EKKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSA 409

[Close]

BLAST of POU domain protein vs. Ensembl Mouse
Match: Pou4f1 (POU domain, class 4, transcription factor 1 [Source:MGI Symbol;Acc:MGI:102525])

HSP 1 Score: 248.825 bits (634), Expect = 1.023e-77
Identity = 120/159 (75.47%), Postives = 134/159 (84.28%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            D + + RELEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE   +E++N  + F+   E+KRKRTSI   EKRSLEA+F +QPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMKFS 
Sbjct:  262 DSDTDPRELEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEGAQREKMNKPELFNG-GEKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFSA 419

[Close]

BLAST of POU domain protein vs. Ensembl Mouse
Match: Pou3f2 (POU domain, class 3, transcription factor 2 [Source:MGI Symbol;Acc:MGI:101895])

HSP 1 Score: 147.132 bits (370), Expect = 6.283e-39
Identity = 77/149 (51.68%), Postives = 104/149 (69.80%), Query Frame = 1

Query:  628 ELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSL-SQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            +LE FA+ FKQRRIKL  TQADVG ALG L     G++ SQ+TICRFE+L LS  NM  LKP+L  WLE+A++ +    +  D  +    +++KRTSI  S K +LE+ F   P+PS+++I  +A+ L L+K VVRVWFCN+RQK+KRM
Sbjct:  271 DLEQFAKQFKQRRIKLGFTQADVGLALGTL----YGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEEADSSSGSPTS-IDKIAAQGRKRKKRTSIEVSVKGALESHFLKCPKPSAQEITSLADSLQLEKEVVRVWFCNRRQKEKRM 414

[Close]

BLAST of POU domain protein vs. UniProt/SwissProt
Match: sp|Q90435|PO4F3_DANRE (POU domain, class 4, transcription factor 3 OS=Danio rerio OX=7955 GN=pou4f3 PE=2 SV=2)

HSP 1 Score: 252.292 bits (643), Expect = 2.584e-79
Identity = 131/207 (63.29%), Postives = 148/207 (71.50%), Query Frame = 1

Query:  484 GSNCPPNVNIXXXXXXXXXXXXXXXXXXXXMQQP-IQAVNQG-----DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTL 1086
            G   PP+ ++      Q             M  P   +V+ G     D+E + RELEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKPVLQ WLE+AE   +E+ NG     + +ERKRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S +
Sbjct:  125 GMGAPPDPSVMTTQAHQHHLQMGHLHQAMAMGHPHTLSVHNGMACVNDVESDPRELEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPVLQAWLEEAEAAYREK-NGKPDLFNGNERKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAV 330

[Close]

BLAST of POU domain protein vs. UniProt/SwissProt
Match: sp|D3ZTL1|PO4F3_RAT (POU domain, class 4, transcription factor 3 OS=Rattus norvegicus OX=10116 GN=Pou4f3 PE=2 SV=1)

HSP 1 Score: 251.906 bits (642), Expect = 4.923e-79
Identity = 122/160 (76.25%), Postives = 135/160 (84.38%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTL 1086
            D+E + RELEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKPVLQ WLE+AE   +E+ +  + F+   ERKRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S +
Sbjct:  179 DVESDPRELEAFAERFKQRRIKLGVTQADVGAALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPVLQAWLEEAEAAYREKNSKPELFNG-SERKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAV 337

[Close]

BLAST of POU domain protein vs. UniProt/SwissProt
Match: sp|Q15319|PO4F3_HUMAN (POU domain, class 4, transcription factor 3 OS=Homo sapiens OX=9606 GN=POU4F3 PE=1 SV=1)

HSP 1 Score: 251.906 bits (642), Expect = 5.139e-79
Identity = 122/160 (76.25%), Postives = 135/160 (84.38%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTL 1086
            D+E + RELEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKPVLQ WLE+AE   +E+ +  + F+   ERKRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S +
Sbjct:  179 DVESDPRELEAFAERFKQRRIKLGVTQADVGAALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPVLQAWLEEAEAAYREKNSKPELFNG-SERKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAV 337

[Close]

BLAST of POU domain protein vs. UniProt/SwissProt
Match: sp|Q63955|PO4F3_MOUSE (POU domain, class 4, transcription factor 3 OS=Mus musculus OX=10090 GN=Pou4f3 PE=1 SV=2)

HSP 1 Score: 251.521 bits (641), Expect = 5.365e-79
Identity = 122/160 (76.25%), Postives = 135/160 (84.38%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTL 1086
            D+E + RELEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKPVLQ WLE+AE   +E+ +  + F+   ERKRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S +
Sbjct:  179 DVESDPRELEAFAERFKQRRIKLGVTQADVGAALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPVLQAWLEEAEAAYREKNSKPELFNG-SERKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAV 337

[Close]

BLAST of POU domain protein vs. UniProt/SwissProt
Match: sp|Q12837|PO4F2_HUMAN (POU domain, class 4, transcription factor 2 OS=Homo sapiens OX=9606 GN=POU4F2 PE=1 SV=2)

HSP 1 Score: 249.595 bits (636), Expect = 2.414e-77
Identity = 119/159 (74.84%), Postives = 134/159 (84.28%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            D++ + R+LEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE   +E+L   + F+   E+KRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S 
Sbjct:  250 DVDADPRDLEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKSHREKLTKPELFNGA-EKKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSA 407

[Close]

BLAST of POU domain protein vs. TrEMBL
Match: A0A0A1CRK9 (POU domain protein (Fragment) OS=Schmidtea mediterranea OX=79327 GN=pou4l-1 PE=2 SV=1)

HSP 1 Score: 275.018 bits (702), Expect = 5.934e-85
Identity = 129/168 (76.79%), Postives = 147/168 (87.50%), Query Frame = 1

Query:  601 QGDIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTLGFQHNS 1104
            Q  + Y+ RELE F+E FKQRRIKL VTQADVG+ALGNLKL+GVGSLSQSTICRFESLTLSHNNM+ALKP+LQ WLE+AE +A ERL   D ++D  +RKRKRTSITD+EKRSLEA+F +QPRPSSEKI QIA+KLNLKKNVVRVWFCNQRQKQKRMKFST+   H+S
Sbjct:  223 QDQLNYDPRELEIFSERFKQRRIKLGVTQADVGKALGNLKLSGVGSLSQSTICRFESLTLSHNNMVALKPILQAWLEEAERQAAERLKNPDIYNDSLDRKRKRTSITDAEKRSLEAYFAVQPRPSSEKIAQIADKLNLKKNVVRVWFCNQRQKQKRMKFSTVNLDHHS 390

[Close]

BLAST of POU domain protein vs. TrEMBL
Match: A0A267ER71 (POU domain protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000992g3 PE=3 SV=1)

HSP 1 Score: 273.863 bits (699), Expect = 4.250e-84
Identity = 130/164 (79.27%), Postives = 144/164 (87.80%), Query Frame = 1

Query:  604 GDIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTLGFQ 1095
            G+++Y+ RELEAFAE FKQRRIKL VTQADVG+ALGNLKL GVGSLSQSTICRFESLTLSHNNMIALKPVLQ WLE+AE  A ERL   D + D  ++KRKRTSITD+EKRSLEA+F +QPRPSSEKI QIAEKLNLKKNVVRVWFCNQRQKQKRMKF++ G  
Sbjct:  253 GELDYDPRELEAFAERFKQRRIKLGVTQADVGKALGNLKLPGVGSLSQSTICRFESLTLSHNNMIALKPVLQAWLEEAEHVAMERLKNPDFYGDGLDKKRKRTSITDAEKRSLEAYFHVQPRPSSEKISQIAEKLNLKKNVVRVWFCNQRQKQKRMKFASPGMH 416

[Close]

BLAST of POU domain protein vs. TrEMBL
Match: A0A094ZFM2 (POU domain protein OS=Schistosoma haematobium OX=6185 GN=MS3_11446 PE=3 SV=1)

HSP 1 Score: 268.47 bits (685), Expect = 4.971e-84
Identity = 134/167 (80.24%), Postives = 150/167 (89.82%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTLGFQHNSS 1107
            +++Y  RELEAF+E FKQRRIKL VTQADVG+ALGNLK++GVGSLSQSTICRFESLTLSHNNM+ALKPVLQ WLE+AE EA  RLN  + +   +ER+RKRTSITDSEKRSLEAFF+IQPRPSSEKI QIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTLG  H+S+
Sbjct:  102 ELDYNPRELEAFSELFKQRRIKLGVTQADVGKALGNLKISGVGSLSQSTICRFESLTLSHNNMVALKPVLQAWLEEAEAEASSRLNHPNIYDLDEERRRKRTSITDSEKRSLEAFFSIQPRPSSEKIAQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTLGMLHHSA 268

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BLAST of POU domain protein vs. TrEMBL
Match: A0A3P8GTS8 (POU domain protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS3607 PE=3 SV=1)

HSP 1 Score: 263.077 bits (671), Expect = 5.775e-83
Identity = 130/168 (77.38%), Postives = 150/168 (89.29%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTLGFQHNSSN 1110
            +++Y  RELEAF+E FKQRRIKL VTQADVG+ALGNLK+AGVGSLSQSTICRFESLTLSHNNM+ALKPVLQ WLE+AE +A ER    + +   +E++RKRTSITDSEKRSLEAFF++QPRPSSEKI QIAEKLNL+KNVVRVWFCNQRQKQKRMKFSTLG  H+++N
Sbjct:   26 EVDYNPRELEAFSELFKQRRIKLGVTQADVGKALGNLKIAGVGSLSQSTICRFESLTLSHNNMVALKPVLQAWLEEAEADAAERSRHPEIYDPEEEKRRKRTSITDSEKRSLEAFFSVQPRPSSEKISQIAEKLNLRKNVVRVWFCNQRQKQKRMKFSTLGLLHHAAN 193

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BLAST of POU domain protein vs. TrEMBL
Match: A0A183A9H2 (POU domain protein OS=Echinostoma caproni OX=27848 PE=3 SV=1)

HSP 1 Score: 263.077 bits (671), Expect = 6.576e-83
Identity = 130/168 (77.38%), Postives = 150/168 (89.29%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTLGFQHNSSN 1110
            +++Y  RELEAF+E FKQRRIKL VTQADVG+ALGNLK+AGVGSLSQSTICRFESLTLSHNNM+ALKPVLQ WLE+AE +A ER    + +   +E++RKRTSITDSEKRSLEAFF++QPRPSSEKI QIAEKLNL+KNVVRVWFCNQRQKQKRMKFSTLG  H+++N
Sbjct:   26 EVDYNPRELEAFSELFKQRRIKLGVTQADVGKALGNLKIAGVGSLSQSTICRFESLTLSHNNMVALKPVLQAWLEEAEADAAERSRHPEIYDPEEEKRRKRTSITDSEKRSLEAFFSVQPRPSSEKISQIAEKLNLRKNVVRVWFCNQRQKQKRMKFSTLGLLHHAAN 193

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BLAST of POU domain protein vs. Ensembl Cavefish
Match: pou4f1 (POU class 4 homeobox 1 [Source:NCBI gene;Acc:103044015])

HSP 1 Score: 246.514 bits (628), Expect = 1.795e-77
Identity = 118/158 (74.68%), Postives = 134/158 (84.81%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFS 1080
            D + + RELEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE   +E+++  + F+   E+KRKRTSI   EKRSLEA+F +QPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKR+KFS
Sbjct:  221 DSDTDPRELEAFAERFKQRRIKLGVTQADVGGALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEGAQREKMSKPEIFNG-GEKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRLKFS 377

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BLAST of POU domain protein vs. Ensembl Cavefish
Match: pou4f2 (POU class 4 homeobox 2 [Source:NCBI gene;Acc:103047395])

HSP 1 Score: 247.669 bits (631), Expect = 2.639e-77
Identity = 118/159 (74.21%), Postives = 134/159 (84.28%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            D++ + R+LEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKP+L  WLE+AE   +E+L+  + F+   E+KRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S 
Sbjct:  264 DVDADPRDLEAFAERFKQRRIKLGVTQADVGAALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILHAWLEEAEKSHREKLSKPELFNGA-EKKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSA 421

[Close]

BLAST of POU domain protein vs. Ensembl Cavefish
Match: zgc:158291 (brain-specific homeobox/POU domain protein 3-like [Source:NCBI gene;Acc:103033167])

HSP 1 Score: 243.047 bits (619), Expect = 1.940e-76
Identity = 116/158 (73.42%), Postives = 132/158 (83.54%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFS 1080
            D E + RELE+FAE FKQRRIKL VTQADVG AL NLK+ GVG LSQSTICRFESLTLSHNNM+ALKP+L+ WLE+AE   +E++   + F+  D +KRKRTSI   EKRSLEA+F +QPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMKFS
Sbjct:  195 DSEPDPRELESFAERFKQRRIKLGVTQADVGAALANLKIPGVGCLSQSTICRFESLTLSHNNMVALKPILEAWLEEAERAQREKMTKPEIFNGAD-KKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFS 351

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BLAST of POU domain protein vs. Ensembl Cavefish
Match: pou4f3 (POU class 4 homeobox 3 [Source:NCBI gene;Acc:103036569])

HSP 1 Score: 236.884 bits (603), Expect = 2.492e-74
Identity = 117/161 (72.67%), Postives = 130/161 (80.75%), Query Frame = 1

Query:  610 IEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERL--NGSDAFSDID-ERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            +E + RELEAFAE FKQRR+KL VTQADVG AL +L + GVG+LSQSTICRFESLTLSHNNMIAL+PVLQ WL++AE E +  L  NG      +  ERKRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL LKKNVVRVWFCNQRQKQKRMK+S 
Sbjct:  171 VESDPRELEAFAERFKQRRVKLGVTQADVGAALASLNMPGVGALSQSTICRFESLTLSHNNMIALRPVLQAWLDEAEAERRRALGANGVAPMGGLGAERKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLELKKNVVRVWFCNQRQKQKRMKYSA 331

[Close]

BLAST of POU domain protein vs. Ensembl Cavefish
Match: POU3F4 (POU class 3 homeobox 4 [Source:NCBI gene;Acc:103039910])

HSP 1 Score: 144.05 bits (362), Expect = 1.132e-38
Identity = 77/149 (51.68%), Postives = 101/149 (67.79%), Query Frame = 1

Query:  628 ELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSL-SQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            ELE FA+ FKQRRIKL  TQADVG ALG L     G++ SQ+TICRFE+L LS  NM  LKP+L  WLE+A++      +  D  +    +++KRTSI  S K  LE  F   P+P++++I  +A+ L L+K VVRVWFCN+RQK+KRM
Sbjct:  185 ELEQFAKQFKQRRIKLGFTQADVGLALGTL----YGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEEADSSTGSP-SSIDKIAAQGRKRKKRTSIEVSVKGVLETHFLKCPKPAAQEISSLADSLQLEKEVVRVWFCNRRQKEKRM 328

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BLAST of POU domain protein vs. Ensembl Sea Lamprey
Match: pou4f2 (POU class 4 homeobox 2 [Source:ZFIN;Acc:ZDB-GENE-040401-1])

HSP 1 Score: 250.366 bits (638), Expect = 7.143e-80
Identity = 121/160 (75.62%), Postives = 133/160 (83.12%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTL 1086
            D E + RELEAFAE FKQRRIKL VTQADVG ALGNLK+ GVGSLSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE   + +    D F+   E+KRKRTSI   EKRSLEA+F +QPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMKFS +
Sbjct:  203 DTEADPRELEAFAERFKQRRIKLGVTQADVGSALGNLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKAHRNKQTKPDLFNG-GEKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFSAV 361

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BLAST of POU domain protein vs. Ensembl Sea Lamprey
Match: pou2f1b (POU class 2 homeobox 1b [Source:ZFIN;Acc:ZDB-GENE-030131-2422])

HSP 1 Score: 134.42 bits (337), Expect = 9.535e-36
Identity = 78/167 (46.71%), Postives = 104/167 (62.28%), Query Frame = 1

Query:  586 IQAVNQGDIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSD-----AFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            IQ  +  D+E    ELE FA+ FKQRRIKL  TQ DVG A+G  KL G    SQ+TI RFE+L LS  NM  LKP+L+ WL D E    +   G+          +  R++KRTSI    + +LE  F    +P+SE++  IAE+L+++K VVRVWFCN+RQK+KR+
Sbjct:  204 IQPEDPRDLE----ELEVFAKMFKQRRIKLGFTQGDVGLAMG--KLYG-NDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDTEHIPADSSPGNQNASPSGLEGLGRRRKKRTSIDTHVRVALEKSFLENCKPTSEELSMIAEQLHMEKEVVRVWFCNRRQKEKRI 363

[Close]

BLAST of POU domain protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002675.1 (pep scaffold:Pmarinus_7.0:GL479714:4410:5816:-1 gene:ENSPMAG00000002443.1 transcript:ENSPMAT00000002675.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 103.99 bits (258), Expect = 1.870e-26
Identity = 60/152 (39.47%), Postives = 89/152 (58.55%), Query Frame = 1

Query:  628 ELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVG-SLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDI-----DERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQK 1065
            E+  FA+ FK RR+ L +TQ  VG+AL     A  G + SQS ICRFE L L+  +   ++P+L+ W+   E EA+ R+ G+   +D       +R+++RTS T      L A F +   P+S ++ QIAE+L   + VVR+WFCN+RQ  K
Sbjct:    7 EIREFAKVFKLRRLALGLTQTQVGQALS----AAEGPAYSQSAICRFEKLDLTPKSAQKMRPILERWM--GEAEARHRM-GTHTLTDFIGSEPSKRRKRRTSFTQEALVILNAHFDVGMHPTSAEMTQIAEQLGYDREVVRIWFCNKRQALK 151

[Close]

BLAST of POU domain protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009070.1 (pep scaffold:Pmarinus_7.0:GL476573:36580:49436:1 gene:ENSPMAG00000008205.1 transcript:ENSPMAT00000009070.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 105.145 bits (261), Expect = 1.289e-25
Identity = 59/151 (39.07%), Postives = 88/151 (58.28%), Query Frame = 1

Query:  628 ELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDI-----DERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQK 1065
            E+  FA  FK RR+ + +TQ  VG+A+ + +     + SQS ICRFE L ++  +   +KPVL+ W+  AE EA++R  G  + SD        ++R+RTS T     +L A+F     PS +++  IAE L  ++ VVRVWFCN+RQ  K
Sbjct:  153 EIREFARAFKIRRLSMGLTQTQVGQAMSSTE---APAYSQSAICRFEKLDITPKSAQKIKPVLERWM--AEAEARQR-TGLQSLSDFVGSEPSRKRRRRTSFTPQALEALNAYFERNTHPSGQEMTAIAEHLCYEREVVRVWFCNKRQMLK 297

[Close]

BLAST of POU domain protein vs. Ensembl Sea Lamprey
Match: pou6f2 (POU class 6 homeobox 2 [Source:ZFIN;Acc:ZDB-GENE-091118-71])

HSP 1 Score: 102.064 bits (253), Expect = 1.195e-24
Identity = 58/148 (39.19%), Postives = 83/148 (56.08%), Query Frame = 1

Query:  628 ELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAF--SDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQK 1065
            E+  FA  FK RR+ L +TQ  VG+AL     A   + SQS ICRFE L ++  +   +KPVL+ W+E+AE   +  L     F   +  +++++RTS T      L A F     PS +++  IAE+L   + VVRVWFCN+RQ  K
Sbjct:  145 EMREFARAFKIRRLSLGLTQTQVGQAL---SAAEGPAYSQSAICRFEKLDITPKSAQKIKPVLERWMEEAEARKQAGLQSLSDFVGGEPSKKRKRRTSFTPQALELLNAHFERNTHPSGQEMTAIAERLCYDREVVRVWFCNKRQALK 289

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BLAST of POU domain protein vs. Ensembl Yeast
Match: YOX1 (Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; phosphorylated by Cdc28p; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication [Source:SGD;Acc:S000004489])

HSP 1 Score: 48.1358 bits (113), Expect = 1.585e-6
Identity = 27/63 (42.86%), Postives = 39/63 (61.90%), Query Frame = 1

Query:  895 RKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            RKR+RTS    E   L+A F   P PS EK I++AE  ++ +  V++WF N+RQ  KR + +T
Sbjct:  178 RKRRRTS--SQELSILQAEFEKCPAPSKEKRIELAESCHMTEKAVQIWFQNKRQAVKRQRIAT 238

[Close]

BLAST of POU domain protein vs. Ensembl Nematostella
Match: EDO47033 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNB5])

HSP 1 Score: 218.394 bits (555), Expect = 5.374e-70
Identity = 112/153 (73.20%), Postives = 125/153 (81.70%), Query Frame = 1

Query:  625 RELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            RELE FAE FKQRRIKL VTQADVG AL +LK+ GVGSLSQSTICRFESLTLSHNNM+ALKPVL  WLE+AE   K +   S AF    ++KRKRTSI  +EKRSLEA+F + PRPSS+KI  IAEKL+L KNVVRVWFCNQRQK+KRMKFS 
Sbjct:    4 RELEWFAERFKQRRIKLGVTQADVGSALAHLKIPGVGSLSQSTICRFESLTLSHNNMMALKPVLTAWLEEAEKAYKTKQCNS-AFLPNSDKKRKRTSIGAAEKRSLEAYFAMNPRPSSDKIASIAEKLDLSKNVVRVWFCNQRQKKKRMKFSV 155

[Close]

BLAST of POU domain protein vs. Ensembl Nematostella
Match: EDO33532 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRV9])

HSP 1 Score: 145.206 bits (365), Expect = 2.169e-41
Identity = 78/149 (52.35%), Postives = 101/149 (67.79%), Query Frame = 1

Query:  628 ELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSL-SQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            +LE FA+ FKQRRIKL  TQADVG ALG L     G++ SQ+TICRFE+L LS  NM  LKP+LQ WLE+A+        G D  +    +++KRTSI  + K +LE  F   P+P++++I  +AE L L K VVRVWFCN+RQK+KRM
Sbjct:   41 DLEQFAKQFKQRRIKLGFTQADVGLALGTL----YGNVFSQTTICRFEALQLSFKNMCKLKPLLQKWLEEADNN-NGATGGVDKLATTGRKRKKRTSIEVAVKGALENHFCKSPKPTAQEISALAENLGLDKEVVRVWFCNRRQKEKRM 184

[Close]

BLAST of POU domain protein vs. Ensembl Nematostella
Match: EDO38767 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC36])

HSP 1 Score: 129.413 bits (324), Expect = 9.709e-36
Identity = 77/153 (50.33%), Postives = 97/153 (63.40%), Query Frame = 1

Query:  625 RELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKR-TSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFS 1080
            RELE FA  FK RRIKL  TQ +VG AL  +        SQ+TICRFE+L LS  N   LKP+L  WLE+AE     R  G+       ER+RKR T+I  + K +LE  F  Q +PSS +I++IA+ L L K VVRVWFCN+RQ++KR+K S
Sbjct:    7 RELEEFATQFKLRRIKLGFTQTNVGAALAAVHGT---DFSQTTICRFENLQLSFKNACKLKPILARWLEEAE-----RSGGACGEKFGAERRRKRRTTIGLAAKEALENHFMKQTKPSSPEIVRIADGLRLDKEVVRVWFCNRRQREKRVKTS 151

[Close]

BLAST of POU domain protein vs. Ensembl Nematostella
Match: EDO43881 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXQ0])

HSP 1 Score: 126.716 bits (317), Expect = 8.656e-35
Identity = 74/158 (46.84%), Postives = 103/158 (65.19%), Query Frame = 1

Query:  628 ELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGS-LSQSTICRFESLTLSHNNMIALKPVLQTWLEDAET-----EAKERLNGSD-AFSDID---ERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            EL  FA+ F+QRR+ L  TQADVG ALG L     G+ LSQ+TICRFE+L LS  NM  LKP+LQ WL++ +      E +++ N S+ AF+  +   +++++RT I  + K  LE  F   PRPS+  I  +AE L L + VVRVWFCN+RQK++R+
Sbjct:    8 ELSTFAKQFRQRRVALGFTQADVGVALGTL----YGNVLSQTTICRFEALQLSLKNMCKLKPLLQKWLQETDASSSGHEGEKQQNPSNFAFATHNGPYKKRKRRTIIEKNVKGVLENHFEKMPRPSTSDISSLAESLGLDREVVRVWFCNRRQKERRV 161

[Close]

BLAST of POU domain protein vs. Ensembl Nematostella
Match: EDO30968 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZ94])

HSP 1 Score: 116.701 bits (291), Expect = 2.917e-31
Identity = 69/149 (46.31%), Postives = 97/149 (65.10%), Query Frame = 1

Query:  655 KQRRIKLRVTQADVGRALGNLKLAGVGS-LSQSTICRFESLTLSHNNMIALKPVLQTWLEDAET-----EAKERLNGSD-AFSDID---ERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            +QRR+ L  TQADVG ALG L     G+ LSQ+TICRFE+L LS  NM  LKP+LQ WL++ +      E +++ N S+ AF+  +   +++++RT I  + K  LE  F   PRPS+  I  +AE L L + VVRVWFCN+RQK++R+
Sbjct:    1 RQRRVALGFTQADVGVALGTL----YGNVLSQTTICRFEALQLSLKNMCKLKPLLQKWLQETDASSSGHEGEKQQNPSNFAFATHNGPYKKRKRRTIIEKNVKGVLENHFEKMPRPSTSDISSLAESLGLDREVVRVWFCNRRQKERRV 145

[Close]

BLAST of POU domain protein vs. Ensembl Medaka
Match: pou4f3 (POU class 4 homeobox 3 [Source:NCBI gene;Acc:101171765])

HSP 1 Score: 255.373 bits (651), Expect = 4.478e-81
Identity = 124/160 (77.50%), Postives = 136/160 (85.00%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTL 1086
            D+E + RELEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKPVLQ WLE+AE   +E+ N  D F+  +ERKRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S +
Sbjct:  201 DVESDPRELEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPVLQAWLEEAEAAYREKSNKPDLFNG-NERKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAV 359

[Close]

BLAST of POU domain protein vs. Ensembl Medaka
Match: pou4f1 (POU class 4 homeobox 1 [Source:NCBI gene;Acc:101156638])

HSP 1 Score: 248.825 bits (634), Expect = 2.268e-78
Identity = 119/158 (75.32%), Postives = 134/158 (84.81%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFS 1080
            D + + RELEAFAE FKQRRIKL VTQADVG AL NLK+ GVGSLSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE   +E+++  D F+   E+KRKRTSI   EKRSLEA+F +QPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKR+KFS
Sbjct:  211 DSDTDPRELEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEGAQREKMSKPDIFNG-GEKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRLKFS 367

[Close]

BLAST of POU domain protein vs. Ensembl Medaka
Match: pou4f2 (POU class 4 homeobox 2 [Source:NCBI gene;Acc:101156468])

HSP 1 Score: 248.44 bits (633), Expect = 3.410e-78
Identity = 119/159 (74.84%), Postives = 135/159 (84.91%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            D++ + R+LEAFAE FKQRRIKL VTQADVG AL +LK+ GVGSLSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE   +E+LN  + F+   E+KRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S 
Sbjct:  222 DVDADPRDLEAFAERFKQRRIKLGVTQADVGSALASLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKSHREKLNKPELFNGA-EKKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSA 379

[Close]

BLAST of POU domain protein vs. Ensembl Medaka
Match: pou4f2 (POU class 4 homeobox 2 [Source:NCBI gene;Acc:101156468])

HSP 1 Score: 247.284 bits (630), Expect = 3.492e-77
Identity = 119/159 (74.84%), Postives = 135/159 (84.91%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            D++ + R+LEAFAE FKQRRIKL VTQADVG AL +LK+ GVGSLSQSTICRFESLTLSHNNMIALKP+LQ WLE+AE   +E+LN  + F+   E+KRKRTSI   EKRSLEA+F IQPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMK+S 
Sbjct:  267 DVDADPRDLEAFAERFKQRRIKLGVTQADVGSALASLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKSHREKLNKPELFNGA-EKKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSA 424

[Close]

BLAST of POU domain protein vs. Ensembl Medaka
Match: zgc:158291 (brain-specific homeobox/POU domain protein 3 [Source:NCBI gene;Acc:101157964])

HSP 1 Score: 242.276 bits (617), Expect = 4.061e-76
Identity = 116/159 (72.96%), Postives = 133/159 (83.65%), Query Frame = 1

Query:  607 DIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFST 1083
            D E + RELE+FAE FKQRRIKL VTQADVG AL NLK+ GVG LSQSTICRFESLTLSHNNM+ALKP+L+ WLE+AE   +E+++  + F+  D +KRKRTSI   EKRSLEA+F +QPRPSSEKI  IAEKL+LKKNVVRVWFCNQRQKQKRMKFS 
Sbjct:  189 DAEPDPRELESFAERFKQRRIKLGVTQADVGAALANLKIPGVGCLSQSTICRFESLTLSHNNMVALKPILEAWLEEAERAQREKMSKPEIFNGGD-KKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFSA 346

[Close]

BLAST of POU domain protein vs. Planmine SMEST
Match: SMESG000030759.1 (SMESG000030759.1)

HSP 1 Score: 692.96 bits (1787), Expect = 0.000e+0
Identity = 355/356 (99.72%), Postives = 355/356 (99.72%), Query Frame = 1

Query:   46 MENGVATSCSLLANGGYHNSASVGWRFPQNNSASDSVENEFISNANLKITSSNIPNFFYQCPENSQSTSNSNGFYSVDTNCSAGFQDMNTYYSRVAAGFNQSFDLIPQHQSFVRLNSTGLSETDQMTYINPNLHSSFHRNISYPNYGSNCPPNVNIXXXXXXXXXXXXXXXXXXXXMQQPIQAVNQGDIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTLGFQHNSSNM 1113
            MENGVATSCSLLANGGYHNSASVGWRFPQNNSASDSVENEFISNANLKITSSNIPNFFYQCPENSQSTSNSNGFYSVDTNCSAGFQDMNTYYSRVAAGFNQSFDLIPQHQSFVRLNSTGLSETDQMTYINPNLHSSFHRNISYPNYGSNCPPNVNIGGVSMVQGSSSSFLGSNYSGMQQPIQ VNQGDIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTLGFQHNSSNM
Sbjct:    1 MENGVATSCSLLANGGYHNSASVGWRFPQNNSASDSVENEFISNANLKITSSNIPNFFYQCPENSQSTSNSNGFYSVDTNCSAGFQDMNTYYSRVAAGFNQSFDLIPQHQSFVRLNSTGLSETDQMTYINPNLHSSFHRNISYPNYGSNCPPNVNIGGVSMVQGSSSSFLGSNYSGMQQPIQVVNQGDIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTLGFQHNSSNM 356

[Close]

BLAST of POU domain protein vs. Planmine SMEST
Match: SMESG000013430.1 (SMESG000013430.1)

HSP 1 Score: 275.018 bits (702), Expect = 1.650e-88
Identity = 129/168 (76.79%), Postives = 147/168 (87.50%), Query Frame = 1

Query:  601 QGDIEYETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTLGFQHNS 1104
            Q  + Y+ RELE F+E FKQRRIKL VTQADVG+ALGNLKL+GVGSLSQSTICRFESLTLSHNNM+ALKP+LQ WLE+AE +A ERL   D ++D  +RKRKRTSITD+EKRSLEA+F +QPRPSSEKI QIA+KLNLKKNVVRVWFCNQRQKQKRMKFST+   H+S
Sbjct:  223 QDQLNYDPRELEIFSERFKQRRIKLGVTQADVGKALGNLKLSGVGSLSQSTICRFESLTLSHNNMVALKPILQAWLEEAERQAAERLKNPDIYNDSLDRKRKRTSITDAEKRSLEAYFAVQPRPSSEKIAQIADKLNLKKNVVRVWFCNQRQKQKRMKFSTVNLDHHS 390

[Close]

BLAST of POU domain protein vs. Planmine SMEST
Match: SMESG000076173.1 (SMESG000076173.1)

HSP 1 Score: 150.599 bits (379), Expect = 2.414e-40
Identity = 79/151 (52.32%), Postives = 106/151 (70.20%), Query Frame = 1

Query:  622 TRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSL-SQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            T ELE FA+ FKQRRIKL  TQADVG ALGNL     GS+ SQ+TICRFE+L LS  NM  L+P+L  WL++A++      +G D  +    +++KRTSI    K +LE+ F+ Q +P+++ I Q+A+ L L+K VVRVWFCN+RQK+KRM
Sbjct:  212 TDELEQFAKIFKQRRIKLGFTQADVGLALGNL----YGSVFSQTTICRFEALQLSFKNMCKLRPLLGKWLQEADSTGCAP-SGIDKVTAAGRKRKKRTSIEVGVKNALESHFSRQSKPTAQIITQLADSLGLEKEVVRVWFCNRRQKEKRM 357

[Close]

BLAST of POU domain protein vs. Planmine SMEST
Match: SMESG000057970.1 (SMESG000057970.1)

HSP 1 Score: 143.28 bits (360), Expect = 4.116e-37
Identity = 77/149 (51.68%), Postives = 104/149 (69.80%), Query Frame = 1

Query:  628 ELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSL-SQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRM 1071
            +LE FA+ FKQRRIKL  TQADVG ALG L     G++ SQ+TICRFE+L LS  NM  L+P+LQ WL +A++ ++   N  D  S    +++KRTSI  + K  LE+ F    +PS++ I  +AEKL+L+K VVRVWFCN+RQK+KR+
Sbjct:  272 DLEQFAKMFKQRRIKLGYTQADVGLALGTL----YGNVFSQTTICRFEALQLSFKNMCKLRPLLQKWLHEADSSSESPTNF-DKISAQSRKRKKRTSIEANVKLILESSFMKLSKPSAQDISSLAEKLSLEKEVVRVWFCNRRQKEKRI 415

[Close]

BLAST of POU domain protein vs. Planmine SMEST
Match: SMESG000008858.1 (SMESG000008858.1)

HSP 1 Score: 138.658 bits (348), Expect = 2.611e-35
Identity = 71/151 (47.02%), Postives = 103/151 (68.21%), Query Frame = 1

Query:  619 ETRELEAFAEWFKQRRIKLRVTQADVGRALGNLKLAGVGSL-SQSTICRFESLTLSHNNMIALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLEAFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKR 1068
            + RELE FA+ FKQRRIKL  TQADVG ALG L     G++ SQ+TICRFE+L LS  NM  L+P+L  WLE+A+      +   +  ++ + +++KRT+I  + K  LE+ F   P+P++  II ++E++ + K VVRVWFCN+RQK+K+
Sbjct:  312 DCRELEQFAKTFKQRRIKLGFTQADVGLALGTL----YGNVFSQTTICRFEALQLSFKNMNKLRPLLSKWLEEADLSLSNTVVNVEKLANQNRQRKKRTNIEMNTKDILESSFNKDPKPTAAHIISLSERILMDKEVVRVWFCNRRQKEKK 458

The following BLAST results are available for this feature:

BLAST of POU domain protein vs. Ensembl Human
BLAST of POU domain protein vs. Ensembl Celegans
BLAST of POU domain protein vs. Ensembl Fly
BLAST of POU domain protein vs. Ensembl Zebrafish
BLAST of POU domain protein vs. Ensembl Xenopus
BLAST of POU domain protein vs. Ensembl Mouse
BLAST of POU domain protein vs. UniProt/SwissProt
BLAST of POU domain protein vs. TrEMBL
BLAST of POU domain protein vs. Ensembl Cavefish
BLAST of POU domain protein vs. Ensembl Sea Lamprey
BLAST of POU domain protein vs. Ensembl Yeast
BLAST of POU domain protein vs. Ensembl Nematostella
BLAST of POU domain protein vs. Ensembl Medaka
BLAST of POU domain protein vs. Planmine SMEST

BLAST of POU domain protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
POU4F3	1.070e-79	76.25	POU class 4 homeobox 3 [Source:HGNC Symbol;Acc:HGN...	[more]
POU4F2	5.027e-78	74.84	POU class 4 homeobox 2 [Source:HGNC Symbol;Acc:HGN...	[more]
POU4F1	1.350e-77	75.47	POU class 4 homeobox 1 [Source:HGNC Symbol;Acc:HGN...	[more]
POU3F2	8.848e-39	51.68	POU class 3 homeobox 2 [Source:HGNC Symbol;Acc:HGN...	[more]
POU3F4	3.076e-38	51.68	POU class 3 homeobox 4 [Source:HGNC Symbol;Acc:HGN...	[more]

BLAST of POU domain protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
unc-86	9.368e-68	66.25	Transcription factor unc-86 [Source:UniProtKB/Swi...	[more]
unc-86	3.464e-67	66.25	Transcription factor unc-86 [Source:UniProtKB/Swi...	[more]
ceh-6	1.262e-35	46.71	Homeobox protein ceh-6 [Source:UniProtKB/Swiss-Pr...	[more]
ceh-18	3.610e-31	38.14	Homeobox protein ceh-18 [Source:UniProtKB/Swiss-P...	[more]
ceh-18	3.739e-31	38.14	Homeobox protein ceh-18 [Source:UniProtKB/Swiss-P...	[more]

BLAST of POU domain protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
acj6	8.938e-77	74.52	gene:FBgn0000028 transcript:FBtr0305192	[more]
acj6	1.041e-76	74.52	gene:FBgn0000028 transcript:FBtr0305191	[more]
acj6	1.303e-76	74.52	gene:FBgn0000028 transcript:FBtr0305193	[more]
acj6	4.397e-76	73.55	gene:FBgn0000028 transcript:FBtr0305190	[more]
acj6	4.453e-76	74.52	gene:FBgn0000028 transcript:FBtr0305196	[more]

BLAST of POU domain protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pou4f3	3.422e-80	63.29	POU class 4 homeobox 3 [Source:NCBI gene;Acc:30534...	[more]
pou4f1	2.566e-78	74.84	POU class 4 homeobox 1 [Source:ZFIN;Acc:ZDB-GENE-0...	[more]
pou4f2	2.811e-78	74.84	POU class 4 homeobox 2 [Source:ZFIN;Acc:ZDB-GENE-0...	[more]
pou4f2	6.029e-78	74.84	POU class 4 homeobox 2 [Source:ZFIN;Acc:ZDB-GENE-0...	[more]
zgc:158291	5.458e-77	73.13	zgc:158291 [Source:ZFIN;Acc:ZDB-GENE-061215-70]	[more]

BLAST of POU domain protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
POU4F1	1.448e-79	76.73	POU class 4 homeobox 1 [Source:NCBI gene;Acc:10048...	[more]
rnf220.1	3.384e-79	75.63	ring finger protein 220, gene 1 [Source:Xenbase;Ac...	[more]
ppp2r2a	1.312e-77	74.84	protein phosphatase 2 regulatory subunit B, alpha ...	[more]
pou4f1.2	3.111e-75	73.72	POU class 4 homeobox 1 [Source:NCBI gene;Acc:10003...	[more]
pou3f2	2.270e-39	51.68	POU class 3 homeobox 2 [Source:NCBI gene;Acc:10048...	[more]

BLAST of POU domain protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Pou4f3	7.626e-81	76.25	POU domain, class 4, transcription factor 3 [Sourc...	[more]
Pou4f3	7.670e-80	76.25	POU domain, class 4, transcription factor 3 [Sourc...	[more]
Pou4f2	5.486e-78	74.84	POU domain, class 4, transcription factor 2 [Sourc...	[more]
Pou4f1	1.023e-77	75.47	POU domain, class 4, transcription factor 1 [Sourc...	[more]
Pou3f2	6.283e-39	51.68	POU domain, class 3, transcription factor 2 [Sourc...	[more]

BLAST of POU domain protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q90435\|PO4F3_DANRE	2.584e-79	63.29	POU domain, class 4, transcription factor 3 OS=Dan...	[more]
sp\|D3ZTL1\|PO4F3_RAT	4.923e-79	76.25	POU domain, class 4, transcription factor 3 OS=Rat...	[more]
sp\|Q15319\|PO4F3_HUMAN	5.139e-79	76.25	POU domain, class 4, transcription factor 3 OS=Hom...	[more]
sp\|Q63955\|PO4F3_MOUSE	5.365e-79	76.25	POU domain, class 4, transcription factor 3 OS=Mus...	[more]
sp\|Q12837\|PO4F2_HUMAN	2.414e-77	74.84	POU domain, class 4, transcription factor 2 OS=Hom...	[more]

BLAST of POU domain protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0A1CRK9	5.934e-85	76.79	POU domain protein (Fragment) OS=Schmidtea mediter...	[more]
A0A267ER71	4.250e-84	79.27	POU domain protein OS=Macrostomum lignano OX=28230...	[more]
A0A094ZFM2	4.971e-84	80.24	POU domain protein OS=Schistosoma haematobium OX=6...	[more]
A0A3P8GTS8	5.775e-83	77.38	POU domain protein OS=Echinostoma caproni OX=27848...	[more]
A0A183A9H2	6.576e-83	77.38	POU domain protein OS=Echinostoma caproni OX=27848...	[more]

BLAST of POU domain protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pou4f1	1.795e-77	74.68	POU class 4 homeobox 1 [Source:NCBI gene;Acc:10304...	[more]
pou4f2	2.639e-77	74.21	POU class 4 homeobox 2 [Source:NCBI gene;Acc:10304...	[more]
zgc:158291	1.940e-76	73.42	brain-specific homeobox/POU domain protein 3-like ...	[more]
pou4f3	2.492e-74	72.67	POU class 4 homeobox 3 [Source:NCBI gene;Acc:10303...	[more]
POU3F4	1.132e-38	51.68	POU class 3 homeobox 4 [Source:NCBI gene;Acc:10303...	[more]

BLAST of POU domain protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pou4f2	7.143e-80	75.63	POU class 4 homeobox 2 [Source:ZFIN;Acc:ZDB-GENE-0...	[more]
pou2f1b	9.535e-36	46.71	POU class 2 homeobox 1b [Source:ZFIN;Acc:ZDB-GENE-...	[more]
ENSPMAT00000002675.1	1.870e-26	39.47	pep scaffold:Pmarinus_7.0:GL479714:4410:5816:-1 ge...	[more]
ENSPMAT00000009070.1	1.289e-25	39.07	pep scaffold:Pmarinus_7.0:GL476573:36580:49436:1 g...	[more]
pou6f2	1.195e-24	39.19	POU class 6 homeobox 2 [Source:ZFIN;Acc:ZDB-GENE-0...	[more]

BLAST of POU domain protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
YOX1	1.585e-6	42.86	Homeobox transcriptional repressor; binds to Mcm1p...	[more]

BLAST of POU domain protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO47033	5.374e-70	73.20	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO33532	2.169e-41	52.35	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO38767	9.709e-36	50.33	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO43881	8.656e-35	46.84	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO30968	2.917e-31	46.31	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of POU domain protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pou4f3	4.478e-81	77.50	POU class 4 homeobox 3 [Source:NCBI gene;Acc:10117...	[more]
pou4f1	2.268e-78	75.32	POU class 4 homeobox 1 [Source:NCBI gene;Acc:10115...	[more]
pou4f2	3.410e-78	74.84	POU class 4 homeobox 2 [Source:NCBI gene;Acc:10115...	[more]
pou4f2	3.492e-77	74.84	POU class 4 homeobox 2 [Source:NCBI gene;Acc:10115...	[more]
zgc:158291	4.061e-76	72.96	brain-specific homeobox/POU domain protein 3 [Sour...	[more]

BLAST of POU domain protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000030759.1	0.000e+0	99.72	SMESG000030759.1	[more]
SMESG000013430.1	1.650e-88	76.79	SMESG000013430.1	[more]
SMESG000076173.1	2.414e-40	52.32	SMESG000076173.1	[more]
SMESG000057970.1	4.116e-37	51.68	SMESG000057970.1	[more]
SMESG000008858.1	2.611e-35	47.02	SMESG000008858.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30002016 ID=SMED30002016|Name=POU domain protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1339bp
GTTGAATTTTTCAAAAACCAAATTTACTTATTTTGAATTGCCATAATGGA
AAATGGAGTTGCAACAAGCTGTTCACTTCTAGCTAATGGTGGATATCATA
ACTCTGCCTCCGTCGGTTGGAGGTTTCCGCAGAACAATTCAGCCAGCGAT
TCCGTTGAGAACGAATTTATATCAAATGCAAATCTCAAAATTACCTCTTC
GAATATTCCAAACTTTTTTTACCAATGTCCGGAAAACAGTCAATCAACGA
GCAATTCTAACGGATTTTATTCAGTGGATACGAATTGCTCGGCTGGATTC
CAAGACATGAATACTTACTATTCAAGGGTTGCAGCAGGCTTCAATCAATC
CTTCGACTTGATACCTCAACACCAGTCTTTTGTGAGGCTCAATTCAACTG
GGCTATCGGAAACTGACCAAATGACTTATATAAATCCAAATCTTCATAGT
TCATTTCATCGAAATATTTCATATCCGAATTACGGTAGTAACTGTCCTCC
TAATGTTAACATAGGAGGAGTTTCCATGGTTCAAGGGTCTTCGTCATCCT
TTTTAGGCTCCAATTATTCAGGCATGCAACAACCAATTCAAGCCGTGAAT
CAAGGTGACATTGAATATGAAACCCGAGAATTAGAAGCTTTTGCTGAATG
GTTTAAACAAAGAAGAATAAAACTGAGAGTGACTCAGGCGGATGTTGGCC
GAGCTCTCGGGAATTTAAAATTAGCCGGTGTTGGATCATTGAGTCAATCA
ACAATTTGTCGGTTTGAATCACTAACTCTCAGTCATAACAATATGATTGC
TCTTAAGCCAGTTTTGCAAACCTGGCTCGAAGACGCTGAAACCGAAGCTA
AGGAAAGATTAAATGGCTCTGATGCTTTCTCTGACATAGATGAAAGAAAA
CGCAAACGAACTTCAATCACAGATTCAGAAAAACGATCACTGGAAGCATT
TTTTACGATTCAACCACGCCCATCAAGCGAGAAAATTATCCAAATTGCGG
AGAAATTGAATTTGAAGAAAAACGTTGTTCGAGTATGGTTTTGTAATCAG
CGCCAAAAACAGAAACGAATGAAGTTTTCCACTCTCGGTTTTCAGCACAA
CTCGTCAAACATGTGAAATCCTATTTCTGTACAGGACAAATACCAAGCAT
TGACCCCTCTGTCTTATTACAAAAAGTGGTATGATTAAAAGTAGAATAAA
AATGATTTTTATTATTTCTTGACCGTAGCTTTTGTTTAAATTGTTTGACT
TCATTTTTACGAAACTGTTATTTTTCCCACTGGACTGAGTCTTAGGTTCA
AATGTGAGATTTGAGGTTGAGCTTTTGCTGAGAATGACA

protein sequence of SMED30002016-orf-1

>SMED30002016-orf-1 ID=SMED30002016-orf-1|Name=SMED30002016-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=357bp
MENGVATSCSLLANGGYHNSASVGWRFPQNNSASDSVENEFISNANLKIT
SSNIPNFFYQCPENSQSTSNSNGFYSVDTNCSAGFQDMNTYYSRVAAGFN
QSFDLIPQHQSFVRLNSTGLSETDQMTYINPNLHSSFHRNISYPNYGSNC
PPNVNIGGVSMVQGSSSSFLGSNYSGMQQPIQAVNQGDIEYETRELEAFA
EWFKQRRIKLRVTQADVGRALGNLKLAGVGSLSQSTICRFESLTLSHNNM
IALKPVLQTWLEDAETEAKERLNGSDAFSDIDERKRKRTSITDSEKRSLE
AFFTIQPRPSSEKIIQIAEKLNLKKNVVRVWFCNQRQKQKRMKFSTLGFQ
HNSSNM*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **molecular function**
Term	Definition
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003700	transcription factor activity, sequence-specific DNA binding
GO:0043565	sequence-specific DNA binding
GO:0046872	metal ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001387	Cro/C1-type_HTH
IPR013847	POU
IPR001356	Homeobox_dom
IPR000327	POU_dom
IPR010982	Lambda_DNA-bd_dom_sf
IPR009057	Homeobox-like_sf
IPR017970	Homeobox_CS

Vocabulary: **biological process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated
GO:0006351	transcription, DNA-templated

Vocabulary: **cellular component**
Term	Definition
GO:0005634	nucleus

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013847	POU domain	PRINTS	PR00028	POUDOMAIN	coord: 207..224 score: 68.4 coord: 249..264 score: 59.7 coord: 283..303 score: 45.34 coord: 233..246 score: 78.72 coord: 318..333 score: 72.2
IPR001356	Homeobox domain	SMART	SM00389	HOX_1	coord: 284..346 e-value: 1.4E-18 score: 77.8
IPR001356	Homeobox domain	PFAM	PF00046	Homeodomain	coord: 285..341 e-value: 8.3E-18 score: 64.0
IPR001356	Homeobox domain	PROSITE	PS50071	HOMEOBOX_2	coord: 282..342 score: 17.572
IPR001356	Homeobox domain	CDD	cd00086	homeodomain	coord: 285..343 e-value: 2.0414E-17 score: 73.4316
IPR000327	POU-specific domain	SMART	SM00352	pou	coord: 188..265 e-value: 1.1E-45 score: 167.8
IPR000327	POU-specific domain	PFAM	PF00157	Pou	coord: 193..265 e-value: 1.4E-28 score: 98.6
IPR000327	POU-specific domain	PROSITE	PS00465	POU_2	coord: 233..246
IPR000327	POU-specific domain	PROSITE	PS51179	POU_3	coord: 188..265 score: 44.121
None	No IPR available	GENE3D	G3DSA:1.10.10.60		coord: 287..334 e-value: 1.6E-20 score: 74.7
None	No IPR available	PANTHER	PTHR11636	POU DOMAIN	coord: 187..346
None	No IPR available	PANTHER	PTHR11636:SF41	POU DOMAIN, CLASS 4, TRANSCRIPTION FACTOR 2	coord: 187..346
IPR010982	Lambda repressor-like, DNA-binding domain superfamily	GENE3D	G3DSA:1.10.260.40		coord: 191..274 e-value: 4.0E-31 score: 108.6
IPR010982	Lambda repressor-like, DNA-binding domain superfamily	SUPERFAMILY	SSF47413	lambda repressor-like DNA-binding domains	coord: 189..265
IPR017970	Homeobox, conserved site	PROSITE	PS00027	HOMEOBOX_1	coord: 317..340
IPR001387	Cro/C1-type helix-turn-helix domain	CDD	cd00093	HTH_XRE	coord: 200..242 e-value: 0.00545253 score: 32.9102
IPR009057	Homeobox-like domain superfamily	SUPERFAMILY	SSF46689	Homeodomain-like	coord: 277..343