Ribonuclease t2

Overview


Name	Ribonuclease t2
Smed ID	SMED30002004
Length (bp)	850

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Ribonuclease t2 (SMED30002004) t-SNE clustered cells

Violin plots show distribution of expression levels for Ribonuclease t2 (SMED30002004) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ribonuclease t2 (SMED30002004) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ribonuclease t2 (SMED30002004) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30002004

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
protonephridia	SMED30002004	SMESG000046228.1	dd_Smed_v4_6454_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30002004	SMESG000046228.1	dd_Smed_v4_6454_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30002004	SMESG000046228.1	dd_Smed_v4_6454_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30002004	SMESG000046228.1	dd_Smed_v6_6454_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
vitelline gland	SMED30002004	SMESG000046228.1	Contig7338	newmark_ests	PMID:28434803 Rouhana et al., 2017
vitelline gland	SMED30002004	SMESG000046228.1	Contig7338	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Human
Match: RNASET2 (ribonuclease T2 [Source:HGNC Symbol;Acc:HGNC:21686])

HSP 1 Score: 80.1073 bits (196), Expect = 4.442e-17
Identity = 53/196 (27.04%), Postives = 90/196 (45.92%), Query Frame = 3

Query:   78 RFASSELFDSLMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWAN-LKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCF-QKQDNEWNLL-SIGICFDR-SMNLKDC 650
            R   +  +  L++   WP + C +    C  P      W IHGLWP K  G     C+   PFN+  +  +  E+   W + + +F     FW +EW KHGTC+ +    +++  YF  +L L + + +  +L    I P+ +  Y +    +A+    G+IP +QC    QD E   +  I +C  +    L++C
Sbjct:   27 RLRDNHEWKKLIMVQHWPETVCEKIQNDCRDPPDY---WTIHGLWPDKSEG-----CNRSWPFNLEEIKDLLPEMRAYWPDVIHSFPNRSRFWKHEWEKHGTCAAQVDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPSIN-YYQVADFKDALARVYGVIPKIQCLPPSQDEEVQTIGQIELCLTKQDQQLQNC 213

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Human
Match: RNASET2 (ribonuclease T2 [Source:HGNC Symbol;Acc:HGNC:21686])

HSP 1 Score: 80.1073 bits (196), Expect = 4.468e-17
Identity = 53/196 (27.04%), Postives = 90/196 (45.92%), Query Frame = 3

Query:   78 RFASSELFDSLMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWAN-LKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCF-QKQDNEWNLL-SIGICFDR-SMNLKDC 650
            R   +  +  L++   WP + C +    C  P      W IHGLWP K  G     C+   PFN+  +  +  E+   W + + +F     FW +EW KHGTC+ +    +++  YF  +L L + + +  +L    I P+ +  Y +    +A+    G+IP +QC    QD E   +  I +C  +    L++C
Sbjct:   27 RLRDNHEWKKLIMVQHWPETVCEKIQNDCRDPPDY---WTIHGLWPDKSEG-----CNRSWPFNLEEIKDLLPEMRAYWPDVIHSFPNRSRFWKHEWEKHGTCAAQVDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPSIN-YYQVADFKDALARVYGVIPKIQCLPPSQDEEVQTIGQIELCLTKQDQQLQNC 213

[Close]

Query:   78 RFASSELFDSLMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWAN-LKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCF-QKQDNEWNLL-SIGICFDR-SMNLKDC 650
            R   +  +  L++   WP + C +    C  P      W IHGLWP K  G     C+   PFN+  +  +  E+   W + + +F     FW +EW KHGTC+ +    +++  YF  +L L + + +  +L    I P+ +  Y +    +A+    G+IP +QC    QD E   +  I +C  +    L++C
Sbjct:   27 RLRDNHEWKKLIMVQHWPETVCEKIQNDCRDPPDY---WTIHGLWPDKSEG-----CNRSWPFNLEEIKDLLPEMRAYWPDVIHSFPNRSRFWKHEWEKHGTCAAQVDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPSIN-YYQVADFKDALARVYGVIPKIQCLPPSQDEEVQTIGQIELCLTKQDQQLQNC 213

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Human
Match: RNASET2 (ribonuclease T2 [Source:HGNC Symbol;Acc:HGNC:21686])

HSP 1 Score: 77.7962 bits (190), Expect = 1.679e-16
Identity = 51/180 (28.33%), Postives = 84/180 (46.67%), Query Frame = 3

Query:  126 WPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWAN-LKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCF-QKQDNEWNLLS-IGICFDR-SMNLKDC 650
            WP + C +    C  P      W IHGLWP K  G     C+   PFN+  +  +  E+   W + + +F     FW +EW KHGTC+ +    +++  YF  +L L + + +  +L    I P+ +  Y +    +A+    G+IP +QC    QD E   +  I +C  +    L++C
Sbjct:    5 WPETVCEKIQNDCRDPPDY---WTIHGLWPDKSEG-----CNRSWPFNLEEIKDLLPEMRAYWPDVIHSFPNRSRFWKHEWEKHGTCAAQVDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPSIN-YYQVADFKDALARVYGVIPKIQCLPPSQDEEVQTIGQIELCLTKQDQQLQNC 175

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Human
Match: AL159163.1 (novel protein)

HSP 1 Score: 67.0106 bits (162), Expect = 8.581e-13
Identity = 45/177 (25.42%), Postives = 76/177 (42.94%), Query Frame = 3

Query:   78 RFASSELFDSLMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWAN-LKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNL 602
            R   +  +  L++   WP + C +    C  P      W IHGLWP K  G     C+   PFN+  +  +  E+   W + + +F     FW +EW KHGTC+ +    +++  YF  +L L + + +                       N+V ++LGI P +  +Q  + + NL
Sbjct:    8 RLRDNHEWKKLIMVQHWPETVCEKIQNDCRDPPDY---WTIHGLWPDKSEG-----CNRSWPFNLEEIKDLLPEMRAYWPDVIHSFPNRSRFWKHEWEKHGTCAAQVDALNSQKKYFGRSLELYRELDL-----------------------NSVLLKLGIKPSINYYQTTEEDLNL 153

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Celegans
Match: rnst-2 (pep chromosome:WBcel235:V:1061595:1067908:1 gene:WBGene00019624.1 transcript:K10C9.3.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:rnst-2)

HSP 1 Score: 88.5817 bits (218), Expect = 2.129e-20
Identity = 57/194 (29.38%), Postives = 96/194 (49.48%), Query Frame = 3

Query:   93 ELFDSLMLSIEWPPSYCAQGNIK----CHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGEP--FNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVK-VELGIIPMLQCFQ-KQDNEWNLLSIGICFDRSMNLKDC 650
            E FD LM +  +P + C   +      C  P      W+IHGLWP   +G+   NC G P  F+ N + SI + L++ W NL      + FW +E+ KHGTC+  +KLF +E  YF   +++  +I +   L +   +  ++K      + NA+  V  G      C + K+  ++ L  I +C ++ + ++DC
Sbjct:   21 EPFDYLMFTTIYPTAVCRADDDSVPESCEIPSGTPQ-WSIHGLWPNFENGSYPQNCRGTPRHFDENLIKSIEDRLVVVWPNLYPKKTIQSFWKHEYDKHGTCAQSEKLFESELAYFTEVMKVFDSIDVAGGLKS---VGPSEKPITSSDLKNALSGVTSGKTFQFHCLRDKKTKQFLLGDIRLCLNKDLTIRDC 210

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Fly
Match: RNaseX25 (gene:FBgn0010406 transcript:FBtr0076752)

HSP 1 Score: 96.2857 bits (238), Expect = 7.748e-23
Identity = 59/195 (30.26%), Postives = 96/195 (49.23%), Query Frame = 3

Query:   99 FDSLMLSIEWPPSYC-----AQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGEP-FNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLS-IGICFDRSMNLKDCQNVE 662
            +D L+ + +WP + C        + +C  P K+   W IHG+WP K+H      C+    F+ + L  I + L   W +LK     E  W +EW KHGTC++  +   NE  YF   L  ++   M ++L   +I P+++    +  I+NA+   LG  P + C     +  + LS I ICF +S+ L DC  ++
Sbjct:   76 WDVLIFTQQWPVTTCYHWREENPDQECSLPQKK-EFWTIHGIWPTKLHQMGPNFCNNSANFDPSKLNPIEDRLETFWPDLKGMDSTEWLWKHEWQKHGTCAMLVEELDNELKYFEQGLTWREEYIMSRILDASDIHPDSNNT--VAAINNAIVKALGKNPSIHCLYDGKHGISYLSEIRICFSKSLELIDCDGIK 267

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Zebrafish
Match: rnaset2 (ribonuclease T2 [Source:NCBI gene;Acc:791890])

HSP 1 Score: 91.2781 bits (225), Expect = 1.592e-21
Identity = 63/212 (29.72%), Postives = 97/212 (45.75%), Query Frame = 3

Query:   60 FILLIVRFASSELFDS-----------LMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQK-QDNEWNLLS-IGICFDRSMNLKDCQ 653
            FI   V F++  L  S           L+L+  WP ++C   + K  +       W +HGLWP     N    C+    FN + +  I  E+   W +L   S P+ FW YEW KHGTC+ K +  ++E  YF   L L     +  +L  + I+P ++K Y ++ +  A+    G+ P +QC    Q  +  +L  I IC DR   L  C+
Sbjct:    3 FIAFAVIFSAVYLCSSAFTHPRGEWTKLILTQHWPQTFCKMEHCKTDFSY-----WTLHGLWP-----NTGVRCNTSWHFNASLIEDILPEMEKFWPDLLEPSSPK-FWNYEWTKHGTCAAKSESLNSEHKYFGKALELYHKFDLNSVLLKNQIVP-SEKHYTLEDVEEAITSAYGVKPKIQCVHPGQGGQVQILGQIEICVDRDFQLMGCE 202

[Close]

Query:   60 FILLIVRFASSELFDS-----------LMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQK-QDNEWNLLS-IGICFDRSMNLKDCQ 653
            FI   V F++  L  S           L+L+  WP ++C   + K  +       W +HGLWP     N    C+    FN + +  I  E+   W +L   S P+ FW YEW KHGTC+ K +  ++E  YF   L L     +  +L  + I+P ++K Y ++ +  A+    G+ P +QC    Q  +  +L  I IC DR   L  C+
Sbjct:    3 FIAFAVIFSAVYLCSSAFTHPRGEWTKLILTQHWPQTFCKMEHCKTDFSY-----WTLHGLWP-----NTGVRCNTSWHFNASLIEDILPEMEKFWPDLLEPSSPK-FWNYEWTKHGTCAAKSESLNSEHKYFGKALELYHKFDLNSVLLKNQIVP-SEKHYTLEDVEEAITSAYGVKPKIQCVHPGQGGQVQILGQIEICVDRDFQLMGCE 202

[Close]

Query:   60 FILLIVRFASSELFDS-----------LMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQK-QDNEWNLLS-IGICFDRSMNLKDCQ 653
            FI   V F++  L  S           L+L+  WP ++C   + K  +       W +HGLWP     N    C+    FN + +  I  E+   W +L   S P+ FW YEW KHGTC+ K +  ++E  YF   L L     +  +L  + I+P ++K Y ++ +  A+    G+ P +QC    Q  +  +L  I IC DR   L  C+
Sbjct:    3 FIAFAVIFSAVYLCSSAFTHPRGEWTKLILTQHWPQTFCKMEHCKTDFSY-----WTLHGLWP-----NTGVRCNTSWHFNASLIEDILPEMEKFWPDLLEPSSPK-FWNYEWTKHGTCAAKSESLNSEHKYFGKALELYHKFDLNSVLLKNQIVP-SEKHYTLEDVEEAITSAYGVKPKIQCVHPGQGGQVQILGQIEICVDRDFQLMGCE 202

[Close]

Query:   60 FILLIVRFASSELFDS-----------LMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQK-QDNEWNLLS-IGICFDRSMNLKDCQ 653
            FI   V F++  L  S           L+L+  WP ++C   + K  +       W +HGLWP     N    C+    FN + +  I  E+   W +L   S P+ FW YEW KHGTC+ K +  ++E  YF   L L     +  +L  + I+P ++K Y ++ +  A+    G+ P +QC    Q  +  +L  I IC DR   L  C+
Sbjct:    3 FIAFAVIFSAVYLCSSAFTHPRGEWTKLILTQHWPQTFCKMEHCKTDFSY-----WTLHGLWP-----NTGVRCNTSWHFNASLIEDILPEMEKFWPDLLEPSSPK-FWNYEWTKHGTCAAKSESLNSEHKYFGKALELYHKFDLNSVLLKNQIVP-SEKHYTLEDVEEAITSAYGVKPKIQCVHPGQGGQVQILGQIEICVDRDFQLMGCE 202

[Close]

Query:   60 FILLIVRFASSELFDS-----------LMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQK-QDNEWNLLS-IGICFDRSMNLKDCQ 653
            FI   V F++  L  S           L+L+  WP ++C   + K  +       W +HGLWP     N    C+    FN + +  I  E+   W +L   S P+ FW YEW KHGTC+ K +  ++E  YF   L L     +  +L  + I+P ++K Y ++ +  A+    G+ P +QC    Q  +  +L  I IC DR   L  C+
Sbjct:    3 FIAFAVIFSAVYLCSSAFTHPRGEWTKLILTQHWPQTFCKMEHCKTDFSY-----WTLHGLWP-----NTGVRCNTSWHFNASLIEDILPEMEKFWPDLLEPSSPK-FWNYEWTKHGTCAAKSESLNSEHKYFGKALELYHKFDLNSVLLKNQIVP-SEKHYTLEDVEEAITSAYGVKPKIQCVHPGQGGQVQILGQIEICVDRDFQLMGCE 202

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Xenopus
Match: rnaset2 (ribonuclease T2 [Source:NCBI gene;Acc:780173])

HSP 1 Score: 59.3066 bits (142), Expect = 8.146e-10
Identity = 42/153 (27.45%), Postives = 68/153 (44.44%), Query Frame = 3

Query:  192 WNIHGLWPYKIHGNDTFNCDGEPFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIGICFDRSMNLKDC 650
            W +HGLWP K    +    +  PF  + +  I  EL   W ++ + +K  + W +EW KHGTC+   +  + +  YF   L L   + +  +L    I+P+  K Y        V+  LG           +N   L  I ICF + + L++C
Sbjct:   60 WTLHGLWPDKAQMCN----NSWPFEYSEIQDILPELNHYWPDILHPNK-SQLWKHEWQKHGTCAASLECLNTQHKYFSKGLELYTKVDLNSVLEKSGIVPST-KYYQ-------VRCYLG-----------ENVQTLGQIEICFTKELQLRNC 188

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Mouse
Match: Rnaset2a (ribonuclease T2A [Source:MGI Symbol;Acc:MGI:1915445])

HSP 1 Score: 82.0333 bits (201), Expect = 7.272e-18
Identity = 52/196 (26.53%), Postives = 101/196 (51.53%), Query Frame = 3

Query:   81 FASSELFDSLMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHG-NDTFNCDGEPFNVNSLISIYEELLLNWAN-LKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIG---ICFDRS-MNLKDC 650
            ++ S  +  L+L+  WPP+ C + N  C      +  W IHGLWP +    N +++     FN++ +  +  ++ + W + +   S   +FW +EW KHGTC+ +    ++E  YF  +L L K I +  +L    I P+ +  Y +    +A+    G++P +QC   +  E ++ ++G   +CF +  ++L++C
Sbjct:   33 WSGSHEWKKLILTQHWPPTVCKEVN-SCQ---DSLDYWTIHGLWPDRAEDCNQSWH-----FNLDEIKDLLRDMKIYWPDVIHRSSNRSQFWKHEWVKHGTCAAQVDALNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPSIN-YYQLADFKDALTRIYGVVPKIQCLMPEQGE-SVQTVGQIELCFTKEDLHLRNC 217

[Close]

Query:   81 FASSELFDSLMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHG-NDTFNCDGEPFNVNSLISIYEELLLNWAN-LKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIG---ICFDRS-MNLKDC 650
            ++ S  +  L+L+  WPP+ C + N  C      +  W IHGLWP +    N +++     FN++ +  +  ++ + W + +   S   +FW +EW KHGTC+ +    ++E  YF  +L L K I +  +L    I P+ +  Y +    +A+    G++P +QC   +  E ++ ++G   +CF +  ++L++C
Sbjct:   33 WSGSHEWKKLILTQHWPPTVCKEVN-SCQ---DSLDYWTIHGLWPDRAEDCNQSWH-----FNLDEIKDLLRDMKIYWPDVIHRSSNRSQFWKHEWVKHGTCAAQVDALNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPSIN-YYQLADFKDALTRIYGVVPKIQCLMPEQGE-SVQTVGQIELCFTKEDLHLRNC 217

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Mouse
Match: Gm49673 (predicted gene, 49673 [Source:MGI Symbol;Acc:MGI:6215117])

HSP 1 Score: 82.0333 bits (201), Expect = 7.272e-18
Identity = 52/196 (26.53%), Postives = 101/196 (51.53%), Query Frame = 3

Query:   81 FASSELFDSLMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHG-NDTFNCDGEPFNVNSLISIYEELLLNWAN-LKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIG---ICFDRS-MNLKDC 650
            ++ S  +  L+L+  WPP+ C + N  C      +  W IHGLWP +    N +++     FN++ +  +  ++ + W + +   S   +FW +EW KHGTC+ +    ++E  YF  +L L K I +  +L    I P+ +  Y +    +A+    G++P +QC   +  E ++ ++G   +CF +  ++L++C
Sbjct:   33 WSGSHEWKKLILTQHWPPTVCKEVN-SCQ---DSLDYWTIHGLWPDRAEDCNQSWH-----FNLDEIKDLLRDMKIYWPDVIHRSSNRSQFWKHEWVKHGTCAAQVDALNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPSIN-YYQLADFKDALTRIYGVVPKIQCLMPEQGE-SVQTVGQIELCFTKEDLHLRNC 217

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Mouse
Match: Gm49721 (predicted gene, 49721 [Source:MGI Symbol;Acc:MGI:6215195])

HSP 1 Score: 82.0333 bits (201), Expect = 7.272e-18
Identity = 52/196 (26.53%), Postives = 101/196 (51.53%), Query Frame = 3

Query:   81 FASSELFDSLMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHG-NDTFNCDGEPFNVNSLISIYEELLLNWAN-LKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIG---ICFDRS-MNLKDC 650
            ++ S  +  L+L+  WPP+ C + N  C      +  W IHGLWP +    N +++     FN++ +  +  ++ + W + +   S   +FW +EW KHGTC+ +    ++E  YF  +L L K I +  +L    I P+ +  Y +    +A+    G++P +QC   +  E ++ ++G   +CF +  ++L++C
Sbjct:   33 WSGSHEWKKLILTQHWPPTVCKEVN-SCQ---DSLDYWTIHGLWPDRAEDCNQSWH-----FNLDEIKDLLRDMKIYWPDVIHRSSNRSQFWKHEWVKHGTCAAQVDALNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPSIN-YYQLADFKDALTRIYGVVPKIQCLMPEQGE-SVQTVGQIELCFTKEDLHLRNC 217

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Mouse
Match: Rnaset2b (ribonuclease T2B [Source:MGI Symbol;Acc:MGI:3702087])

HSP 1 Score: 82.0333 bits (201), Expect = 7.272e-18
Identity = 52/196 (26.53%), Postives = 101/196 (51.53%), Query Frame = 3

Query:   81 FASSELFDSLMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHG-NDTFNCDGEPFNVNSLISIYEELLLNWAN-LKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIG---ICFDRS-MNLKDC 650
            ++ S  +  L+L+  WPP+ C + N  C      +  W IHGLWP +    N +++     FN++ +  +  ++ + W + +   S   +FW +EW KHGTC+ +    ++E  YF  +L L K I +  +L    I P+ +  Y +    +A+    G++P +QC   +  E ++ ++G   +CF +  ++L++C
Sbjct:   33 WSGSHEWKKLILTQHWPPTVCKEVN-SCQ---DSLDYWTIHGLWPDRAEDCNQSWH-----FNLDEIKDLLRDMKIYWPDVIHRSSNRSQFWKHEWVKHGTCAAQVDALNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPSIN-YYQLADFKDALTRIYGVVPKIQCLMPEQGE-SVQTVGQIELCFTKEDLHLRNC 217

[Close]

BLAST of Ribonuclease t2 vs. UniProt/SwissProt
Match: sp|Q7M456|RNOY_CRAGI (Ribonuclease Oy OS=Crassostrea gigas OX=29159 PE=1 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 3.924e-29
Identity = 65/194 (33.51%), Postives = 99/194 (51.03%), Query Frame = 3

Query:  108 LMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYK-IHGNDTFNCDGE-PFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQC-FQKQDNEWNLL-SIGICFDRSMNLKDCQNVELPQSM 677
               + +WP + CA+ +  C  P   + GW IHGLWP          NC+G  PF++N+++ +  EL   W NL   +K   FW +EW KHGTC+      SNE  YF   L+L    ++ ++L N  ILP+    Y I +   AVK ELG+  +++C + K+  +  LL  I IC  +   L  C   E+ ++ 
Sbjct:    6 FTFAQQWPIAVCAE-HKSCFIP-DSVVGWGIHGLWPSSDTESKGPENCNGSWPFDINNVMPLVPELKKYWPNLYPDTKANSFWEHEWSKHGTCATSLPATSNELKYFGMGLKLHAKYNISRILVNQGILPSKTAGYMINETEAAVKRELGVDAVIECVYDKEKTKKQLLYEISICLTKEFELISCNKKEVSETT 197

[Close]

BLAST of Ribonuclease t2 vs. UniProt/SwissProt
Match: sp|P80022|RNLE_SOLLC (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 102.449 bits (254), Expect = 7.958e-25
Identity = 66/210 (31.43%), Postives = 98/210 (46.67%), Query Frame = 3

Query:   27 MSLNIMVKRVLFILLIVRFAS----SELFDSLMLSIEWPPSYCAQGNIKCHYPI--KRIHGWNIHGLWPYKIHGNDTFNCD-GEPFNVNSLISIYEELLLNWANLKNFSKP-EEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIGICFDRS 632
            M+ N      L +L+I +  S    ++ FD      +WP SYC      C YP   K    + IHGLWP    G    NCD   P++ + +  +   +  NW  L   S     FW +EW KHGTC+  + + +N+  YF   L LK  I +  +L   +I P+ + +Y++  I NA+K  +G  P +QC   Q     L  + IC D S
Sbjct:    1 MASNSAFSLFLILLIITQCLSVLNAAKDFDFFYFVQQWPGSYCDTKQ-SCCYPTTGKPAADFGIHGLWPNNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCA--ESVLTNQHAYFKKALDLKNQIDLLSILQGADIHPDGE-SYDLVNIRNAIKSAIGYTPWIQCNVDQSGNSQLYQVYICVDGS 206

[Close]

BLAST of Ribonuclease t2 vs. UniProt/SwissProt
Match: sp|P42813|RNS1_ARATH (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 2.258e-24
Identity = 67/210 (31.90%), Postives = 105/210 (50.00%), Query Frame = 3

Query:   15 KIILMSLNIMVKRVLFILLIVRFASSELFDSLMLSIEWPPSYCAQGNIKCHYP--IKRIHGWNIHGLWPYKIHGNDTFNCDG-EPFNVNSLISIYEELLLNWANLKNFSKP-EEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIGICFDRS 632
            KI+L SL ++   V+   +    +SSE FD      +WP SYC     KC YP   K    + IHGLWP    G    NCD  +PF+ +++  +   +  +W  L   S   E FW +EW KHGTCS   +   ++  YF   L LK+  ++   L+   I P+  K+Y+++ I +++K  +G  P ++C +       L  + +C DRS
Sbjct:    2 KILLASLCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYC-DTQKKCCYPNSGKPAADFGIHGLWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCS---ESVIDQHEYFQTALNLKQKTNLLGALTKAGINPDG-KSYSLESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDRS 206

[Close]

BLAST of Ribonuclease t2 vs. UniProt/SwissProt
Match: sp|Q7M438|RNDI_DICDI (Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3)

HSP 1 Score: 97.4413 bits (241), Expect = 4.726e-23
Identity = 63/219 (28.77%), Postives = 107/219 (48.86%), Query Frame = 3

Query:   42 MVKRVLFILLIVRFASSEL----------FDSLMLSIEWPPSYC-AQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGEPFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIGICFDR-SMNLKDCQNVE 662
            ++  +L +LLI   A S +          FD  +   +W  SYC +Q  I+     K    + IHGLWP    G+    C G  FNVN++  + ++L  +W +L   +   +FW +E+ KHGTCSI   + ++   YF   ++L    ++   L + NI P++   Y    I NA+    G  P +QC   Q     L ++ +C D+ S+++ DC +++
Sbjct:    3 LIAALLSVLLIASTAQSTVTIYESSKPGDFDFYLFVQQWIYSYCDSQTCIQ----NKEREAFTIHGLWPENSDGSYPSFCSGPSFNVNAIQDLEDQLNFDWPSLTGPNT--DFWTHEFSKHGTCSITGPI-TDIHDYFATGIKLYTEFNITAALESENIYPSDSNTYKPVDITNAITTHFGGKPGIQCSSGQ-----LSTVAVCIDKNSLSIMDCPDLQ 209

[Close]

BLAST of Ribonuclease t2 vs. UniProt/SwissProt
Match: sp|P83618|RN28_PANGI (Ribonuclease-like storage protein OS=Panax ginseng OX=4054 PE=1 SV=2)

HSP 1 Score: 97.4413 bits (241), Expect = 6.573e-23
Identity = 55/182 (30.22%), Postives = 89/182 (48.90%), Query Frame = 3

Query:  108 LMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGEPFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIGICFD-RSMNLKDC 650
              L ++WP  +C   N  C      ++ + IHGL+PY   G     CDG  F+VNS+     E+ L W + +  ++  +FW +EW KHG CS   +    +  YF   L  +K   +  LL+   I PNND  Y  + I  A+K  L  +P +   + +++E+ L  I +C + ++    DC
Sbjct:   32 FALRLQWPAGFCEVNN-ACDTK-SLLNTFTIHGLYPYNAKGTPALYCDGTAFDVNSVSDFLAEMHLAWPSHETNTEDIQFWEHEWKKHGRCS---EALLKQTDYFRTALAFRKAFDIVGLLNQEGIYPNND-LYRPKMIKEAIKKHLNAVPEIDFTKNENSEYVLTDINVCVNQQATRFVDC 207

[Close]

BLAST of Ribonuclease t2 vs. TrEMBL
Match: V4APT3 (Uncharacterized protein (Fragment) OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_76871 PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 8.563e-33
Identity = 66/191 (34.55%), Postives = 107/191 (56.02%), Query Frame = 3

Query:   87 SSELFDSLMLSIEWPPSYCAQGNIKCHY--PIKRIHGWNIHGLWPYKIHGNDTFNCDGEPFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVEL-GIIPMLQCFQKQDNEWNLLSIGICFDRSMNLKDC 650
            SSE FD   LS  WP + C +G  +  Y      + GW IHGLWP +I G +  +C    FN+ +L  +  +LL  W NL  +S  +  W +EW+KHGTC++ D   + EF +F   L + K I++ +++ N NI+P++ K Y+++ I   +K    GI P++ C   ++  + L  I +C+D+  N+ DC
Sbjct:    1 SSEEFDFFTLSSAWPKTVCLEGQTEKLYCNNTLDVAGWTIHGLWPSRIDGKEPKSCGHIKFNLKTLDPLMGDLLKLWPNLFKYSSLDSLWRHEWNKHGTCAVSDNNTATEFLFFKKALDVFKKINISRVMKNLNIVPSDTKLYSVRTISRGLKKHFSGIEPIIMCLDIKNKTY-LHQIQVCYDKEFNMVDC 190

[Close]

BLAST of Ribonuclease t2 vs. TrEMBL
Match: A0A026WAK0 (Ribonuclease Oy OS=Ooceraea biroi OX=2015173 GN=DMN91_003010 PE=3 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 3.164e-30
Identity = 69/200 (34.50%), Postives = 105/200 (52.50%), Query Frame = 3

Query:   78 RFASSELFDSLMLSIEWPPSYC-----AQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGEPFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQ-KQDNEWNLLSIGICFDRSMNLKDCQNV 659
            RF S+E FD LM +  WPP+ C        +  C  P +    W IHG+WP K H      C+  PFN N L  +  EL  NW +++N S+P  FW +EW KHGTC+I  +  ++E+ YF   L L    +M  +L+  +ILP   + Y +Q I   ++  LG    + C + ++  E  ++ I  CFD+++ L DC  +
Sbjct:   29 RFTSNE-FDVLMFTQRWPPTACFVWKENSESRSCSLPKR--EEWTIHGIWPTKYHTIGPEYCNDLPFNPNMLAPLESELKENWIDIQNGSRPYTFWKHEWDKHGTCAITVQALNSEYNYFLAALNLLDTYNMIDVLAKASILPG--QRYMVQDILAGIEKVLGKRSQVMCAKNEKTGESYMVEIRTCFDKTLQLVDCDGI 223

[Close]

BLAST of Ribonuclease t2 vs. TrEMBL
Match: A0A4Z2DFD7 (Ribonuclease Oy isoform 2 OS=Schistosoma japonicum OX=6182 GN=EWB00_001528 PE=3 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 2.174e-29
Identity = 66/207 (31.88%), Postives = 103/207 (49.76%), Query Frame = 3

Query:   57 LFILLIVRFA------SSELFDSLMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIGICFDRSMNLKDCQN 656
            L ++L+ RF           +D L+ S+EWPP+YC     K  Y    I+ +NIHGLWP         NC    PF ++++  IY EL   WANL +F  P+ FW +EW KHG C++ D + SNE  YF  +L +K  +++ + L +  I+P++        + N +K    +  ++ CF        L  I +C + SM    C +
Sbjct:    4 LLLILVARFVLCTHVCGERQWDYLVYSLEWPPTYCFTHTCKLPY---NINNFNIHGLWPSIWPSGSPTNCPNHMPFEIDTIKPIYTELQKEWANLDDFDDPKAFWKHEWQKHGVCALSDPIISNELDYFNISLIMKSKVNLLRRLESIKIIPSDSVTLKRDVLLNQLKKLFNVDVLMYCFLIHHKPAKLAEIRLCLNPSMEFISCPS 207

[Close]

BLAST of Ribonuclease t2 vs. TrEMBL
Match: A0A4Z2DG04 (Ribonuclease Oy isoform 1 OS=Schistosoma japonicum OX=6182 GN=EWB00_001528 PE=3 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 3.675e-29
Identity = 66/205 (32.20%), Postives = 102/205 (49.76%), Query Frame = 3

Query:   57 LFILLIVRFA------SSELFDSLMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIGICFDRSMNLKDC 650
            L ++L+ RF           +D L+ S+EWPP+YC     K  Y    I+ +NIHGLWP         NC    PF ++++  IY EL   WANL +F  P+ FW +EW KHG C++ D + SNE  YF  +L +K  +++ + L +  I+P++        + N +K    +  ++ CF        L  I +C + SM    C
Sbjct:   24 LLLILVARFVLCTHVCGERQWDYLVYSLEWPPTYCFTHTCKLPY---NINNFNIHGLWPSIWPSGSPTNCPNHMPFEIDTIKPIYTELQKEWANLDDFDDPKAFWKHEWQKHGVCALSDPIISNELDYFNISLIMKSKVNLLRRLESIKIIPSDSVTLKRDVLLNQLKKLFNVDVLMYCFLIHHKPAKLAEIRLCLNPSMEFISC 225

[Close]

BLAST of Ribonuclease t2 vs. TrEMBL
Match: A0A564Y0S6 (Uncharacterized protein (Fragment) OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS1229 PE=3 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 7.734e-29
Identity = 67/188 (35.64%), Postives = 99/188 (52.66%), Query Frame = 3

Query:   93 ELFDSLMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNC-DGEPFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKD-KLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIGICFDRSMNLKDC 650
            E +D L LS  WPP YC     KC YP +RI G+NIHGLW           C +G  F  +S+  IYE+L   W + +++  P  FW +EW KHG C I++  + +N+  YF  +L LK   +  ++   ++I+P+N   YN   I  A+K ++G    L C +K   E  L+   IC ++   L DC
Sbjct:   44 ETWDYLTLSQSWPPGYCQ--FTKCAYP-RRISGFNIHGLWAEIWPDQPMNQCSNGSKFENSSIQDIYEQLKNEWVDERSYDNPSAFWHHEWSKHGVCVIQNNDVITNQHDYFTASLMLKGMYNFTQIFERNHIVPSNTTLYNTASIMEAIKSDIGANVWLHCSKKSGEEAKLVEARICVNKYFKLIDC 228

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Cavefish
Match: rnaset2 (ribonuclease T2 [Source:NCBI gene;Acc:103036121])

HSP 1 Score: 83.9593 bits (206), Expect = 8.264e-19
Identity = 59/231 (25.54%), Postives = 103/231 (44.59%), Query Frame = 3

Query:   42 MVKRVLFILLIVRFASS------ELFDSLMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCF--QKQDNEWNLLSIGICFDRSMNLKDCQNV--ELPQSMDSIIGNLI 701
            M    +F+ L    ASS        +  L+L+  WP ++C       ++       W +HG WP     N    C+    FN + +  +  E+   W +L   +   +FW +EW KHGTC+ KD+   ++  YF   L L   + +  +L    I+P ++K Y +  +  ++    G+ P +QC    K+     +  I IC D+   L DC+    EL  +++ I+  L+
Sbjct:    1 MKTLTIFMCLGFALASSVHMYNRRDWTKLILTHHWPKTFCMIDQCTTNFSY-----WTLHGFWP-----NVGNMCNSSWHFNASLIEDLLPEMKTYWPDLLKPAS-TQFWKHEWEKHGTCAAKDEDLDSQHKYFSKALELYHKLDLDGVLKKSQIVP-SEKYYKLADLEESIVSSFGVTPKIQCVLEAKRAEVQTVGQIEICVDKQFQLTDCEKTTDELQITVNEILPYLL 219

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Cavefish
Match: rnaset2l (ribonuclease Oy-like [Source:NCBI gene;Acc:103022964])

HSP 1 Score: 82.0333 bits (201), Expect = 5.572e-18
Identity = 56/207 (27.05%), Postives = 100/207 (48.31%), Query Frame = 3

Query:   99 FDSLMLSIEWPPSYCAQGNIK--CHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGEPFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCF--QKQDNEWNLLSIGICFDRSMNLKDCQNVELPQSMDSIIGNLIKK 707
            +  ++ +I+WP S+C     +  C  P + IH W IHGLWP + +G     C   P   + L  +  EL   W +L        FW  EW KHGTC+   +   +   YF  +L+L+    +   L+N  I P+ + +Y  ++IH A+   LG   ++QC   +K+   W  L I I  + ++  +  + ++  ++ ++ +G+   K
Sbjct:   44 WTCMIFTIQWPGSFCLSLENRNICRIP-ENIHNWTIHGLWPIRTYGC----CFCWPVFQSHLQDLEPELSQLWPSLIKSKSNFSFWKDEWFKHGTCAACVESMGSPAHYFRTSLKLRTLFDVDNALANTGIKPSCNISYKHEEIHAALGPFLGNNYVVQCVKDEKEREVWVQLKIYISKNITLGCQKEETLKFSRASNTSLGHPCPK 245

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Sea Lamprey
Match: rnaset2 (ribonuclease T2 [Source:NCBI gene;Acc:791890])

HSP 1 Score: 90.1225 bits (222), Expect = 1.081e-21
Identity = 61/217 (28.11%), Postives = 107/217 (49.31%), Query Frame = 3

Query:   27 MSLNIMVKRVLFILLIVRFASS----ELFDSLMLSIEWPPSYC-AQGNIK--CHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGEPFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQ--KQDNEWNLLSIGICFDRSMNLKDC 650
            M+ +I +  V+F++  V   S     E +  L+L+  WP S+C +Q + K  C  P   +  W+IHGLW      + TF      F+V  +  +  +L   W +L+  +   + W +EW +HGTC+   +  +++  YF  T+ L + + + K LS+  + P+N   Y  +QI  A     G+   LQC    +++N   L  I +C+ R  +L +C
Sbjct:    1 MAGHIQLLAVIFVIAAVSVPSYARRYEDWSQLILTQHWPQSFCMSQWSEKHNCTIP-SSVKSWSIHGLWT----NHRTFCNKSWHFDVKEIEDLLPQLDQFWPDLEK-TNGTDLWKHEWIRHGTCAATLEALNSQHNYFSKTMELYQKLDISKALSDAGVTPSNTVFYKEEQILEAFSTAFGVKAKLQCLTVPQEENTQLLGQIQMCWSREFSLVNC 211

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Yeast
Match: RNY1 (Vacuolar RNase of the T(2) family; relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; required for tRNA-specific translational pausing suring oxidative stress; promotes apoptosis under stress conditions and this function is independent of Rny1p catalytic activity [Source:SGD;Acc:S000006044])

HSP 1 Score: 48.521 bits (114), Expect = 5.066e-7
Identity = 43/152 (28.29%), Postives = 69/152 (45.39%), Query Frame = 3

Query:  183 IHGWNIHGLWPYKIHG------NDTFNCDG-------EPFNVN-SLISIYEELLLNWANL---KNFSKPEEFWGYEWHKHGTC--SIKDKLFSN-----------EFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVEL 548
            +  + IHGLWP   HG      N +   D        + FN N + + I  E LL + +L    N    E  W +E++KHGTC  +I+ + ++             + YF  T  L K +     L  +NI+P+ D +Y+++QI  A+  E 
Sbjct:   81 LDSFTIHGLWPDNCHGGYQQFCNRSLQIDDVYYLLHDKKFNNNDTSLQISGEKLLEYLDLYWKSNNGNHESLWIHEFNKHGTCISTIRPECYTEWGANSVDRKRAVYDYFRITYNLFKKLDTFSTLEKNNIVPSVDNSYSLEQIEAALSKEF 232

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Nematostella
Match: EDO48543 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ98])

HSP 1 Score: 104.375 bits (259), Expect = 8.209e-27
Identity = 61/222 (27.48%), Postives = 101/222 (45.50%), Query Frame = 3

Query:   54 VLFILLIVRFAS------SELFDSLMLSIEWPPSYC--------------AQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWANLKNFSK--PEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIGICFDRSMNLKDC 650
            V+  L+I+  AS      S  +D  + S  WP S C                GN +       +  W +HGLWP      +  NC+   PF  + +  + + ++  W    + SK    + W +EW KHGTC+      SNE  YF   L L  N  + + L++ NI+P++D+ Y ++Q+  A+  + G    + C +  D++  L  I IC D+S   ++C
Sbjct:    6 VIATLVIILTASQAFSLESHKWDYFVFSQWWPQSQCYYRNGQTMDDKWRFTSGNARNDCVPADVTTWTLHGLWPTVGGKAEPVNCNSSWPFVESEIQDLEDRMMQRWLAFPDSSKSSARDLWSHEWKKHGTCATDLAQTSNEHSYFSMALALNSNCGLLRALASENIIPSDDQMYTVKQVERAISNKYGAKGRVICLRGPDDQQLLAGIRICLDKSFRPRNC 227

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Nematostella
Match: EDO48544 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ99])

HSP 1 Score: 90.5077 bits (223), Expect = 1.636e-21
Identity = 59/226 (26.11%), Postives = 97/226 (42.92%), Query Frame = 3

Query:   75 VRFASSELFDSLMLSIEWPPSYC--------------AQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWANLKNFSK--PEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQ-----KQ-DNEWNLLSIGICFDRSMNLKDCQNVELPQSMDS 683
            +R   S  +D  + S  WP S C                GN +       +  W +HGLWP      +  NC+   PF  + +  + + ++  W    + SK    + W +EW KHGTC+      SNE  YF   L L  N  + + L++ NI+P++D+ Y ++Q+  A+  + G    + C +     KQ  +E  L  + +   R +NL+     E     DS
Sbjct:   12 IRSLESHKWDYFVFSQWWPQSQCYYRNGQTMDDKWRFTSGNARNDCVPADVTTWTLHGLWPTVGGKAEPVNCNSSWPFVESEIQDLEDRMMQRWLAFPDSSKSSARDLWSHEWKKHGTCATDLAETSNEHSYFSMALALNSNCGLLRALASENIIPSDDQMYTVKQVERAISNKYGAKGRVICLRGPARDKQLSDEPKLCHVMLLLKRRVNLRLASATEDEAPRDS 237

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Medaka
Match: rnaset2 (ribonuclease T2 [Source:NCBI gene;Acc:101163650])

HSP 1 Score: 96.2857 bits (238), Expect = 2.127e-23
Identity = 64/214 (29.91%), Postives = 105/214 (49.07%), Query Frame = 3

Query:   33 LNIMVKRVLFILLIVRFAS----SELFDSLMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQ--KQDNEWNLLSIGICFDRSMNLKDCQ 653
            + + V  +L +   V +A+      ++  L+L+  WP ++C+  +  CH     I  W +HGLWP K       +C+    FN + +  +  ++  +W +L+N S    FW YEWHKHGTC+ K +  +++  YF   L L   + +  +L    I P +DK Y   Q+  A++   G  P +QC    K  +  +L  I ICFD    L DC+
Sbjct:    1 MRLHVLSLLALCACVSWAAVISPHHMWSKLILTHHWPNTFCSMEH--CH---PNISYWTLHGLWPDK-----GMDCNSSWHFNPSLIEDLLPDMNKSWPDLRNRSS-VSFWKYEWHKHGTCAAKAESLNSQHKYFSKALELYHKLDLSSVLVKFGITP-SDKYYPFSQVEGAIENVYGFKPKIQCVHSSKGGDFQSLGQIEICFDSDFTLMDCE 202

[Close]

Query:   33 LNIMVKRVLFILLIVRFAS----SELFDSLMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGE-PFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQ--KQDNEWNLLSIGICFDRSMNLKDCQ 653
            + + V  +L +   V +A+      ++  L+L+  WP ++C+  +  CH     I  W +HGLWP K       +C+    FN + +  +  ++  +W +L+N S    FW YEWHKHGTC+ K +  +++  YF   L L   + +  +L    I P +DK Y   Q+  A++   G  P +QC    K  +  +L  I ICFD    L DC+
Sbjct:    1 MRLHVLSLLALCACVSWAAVISPHHMWSKLILTHHWPNTFCSMEH--CH---PNISYWTLHGLWPDK-----GMDCNSSWHFNPSLIEDLLPDMNKSWPDLRNRSS-VSFWKYEWHKHGTCAAKAESLNSQHKYFSKALELYHKLDLSSVLVKFGITP-SDKYYPFSQVEGAIENVYGFKPKIQCVHSSKGGDFQSLGQIEICFDSDFTLMDCE 202

[Close]

BLAST of Ribonuclease t2 vs. Ensembl Medaka
Match: rnaset2l (ribonuclease Oy [Source:NCBI gene;Acc:101170531])

HSP 1 Score: 84.3445 bits (207), Expect = 9.352e-19
Identity = 46/180 (25.56%), Postives = 86/180 (47.78%), Query Frame = 3

Query:  108 LMLSIEWPPSYCAQ--GNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGEPFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIGICFDRSMNL 641
            ++ +++WP  +CA       C  P   +  W IHGLWP  +H     NC   P   + +  + +EL+ NW +L       +FW  EW KHG C+   + F++   YF  +L+L+K   ++++L +  I P+ ++ Y + ++ + +   +G    +QC     +      + I   R + L
Sbjct:   52 IIFTLQWPGGFCASLYNQTLCRIP-DTVTDWTIHGLWP--LHAQRCCNC--WPMFQSDVQELKDELVENWPSLLKTKSSFQFWEQEWAKHGACAACVEGFNSPQKYFQISLKLRKWFEIQRVLEDAGIAPSCERRYKVAEVQSILTPHVGDQYEIQCVLDHKDRQVWFQVKIRLSRDLTL 226

[Close]

Query:  108 LMLSIEWPPSYCAQ--GNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGEPFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIGICFDRSMNL 641
            ++ +++WP  +CA       C  P   +  W IHGLWP  +H     NC   P   + +  + +EL+ NW +L       +FW  EW KHG C+   + F++   YF  +L+L+K   ++++L +  I P+ ++ Y + ++ + +   +G    +QC     +      + I   R + L
Sbjct:   52 IIFTLQWPGGFCASLYNQTLCRIP-DTVTDWTIHGLWP--LHAQRCCNC--WPMFQSDVQELKDELVENWPSLLKTKSSFQFWEQEWAKHGACAACVEGFNSPQKYFQISLKLRKWFEIQRVLEDAGIAPSCERRYKVAEVQSILTPHVGDQYEIQCVLDHKDRQVWFQVKIRLSRDLTL 226

[Close]

BLAST of Ribonuclease t2 vs. Planmine SMEST
Match: SMESG000046228.1 (SMESG000046228.1)

HSP 1 Score: 397.512 bits (1020), Expect = 1.470e-141
Identity = 194/202 (96.04%), Postives = 196/202 (97.03%), Query Frame = 3

Query:  192 WNIHGLWPYKIHGNDTFNCDGEPFNVN-SLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIGICFDRSMNLKDCQNVELPQSMDSIIGNLIKKIFLYLGAKKVITPIKDKCEGHDFKIPSFE 794
            WNIHGLWPYKIHGNDTFNCDGEPF     LISIYEELLLNWANLKNFSKPE+FWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNA+KVELGIIPMLQCFQKQDNEWNLLSIGICFDRSMNLKDCQNVELPQSM SIIGNLIKKIFLYLGAKKVITPIKDKCEGHDFKIPSFE
Sbjct:   20 WNIHGLWPYKIHGNDTFNCDGEPFQRQLVLISIYEELLLNWANLKNFSKPEKFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAIKVELGIIPMLQCFQKQDNEWNLLSIGICFDRSMNLKDCQNVELPQSMGSIIGNLIKKIFLYLGAKKVITPIKDKCEGHDFKIPSFE 221

[Close]

BLAST of Ribonuclease t2 vs. Planmine SMEST
Match: SMESG000016389.1 (SMESG000016389.1)

HSP 1 Score: 161.77 bits (408), Expect = 2.570e-49
Identity = 74/176 (42.05%), Postives = 116/176 (65.91%), Query Frame = 3

Query:   51 RVLFILLIVRFASSELFDSLMLSIEWPPSYCAQGNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNCDGEPFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQ 578
            ++L IL +V  + S  +D L  ++ WP ++C     +C+Y   ++ GWNIHGLWP  I G D   C G+PFN+N L SI+ +L++ WANL  F+ P EFW +EW KHG C+I   LF+NE  YF  +L+LK+ +S+ K+LS+++I P++DK Y+   +  +V  +LG++P L+C +
Sbjct:    3 KLLIILSVVGVSLSANWDYLTYTLLWPQTFCKYN--QCNYDKTKVTGWNIHGLWPSNITGKDPSKCKGDPFNINDLNSIFPDLVVQWANLLEFTNPAEFWTHEWTKHGLCAILPPLFTNELSYFSRSLQLKQFVSLDKILSDNDIQPSDDKQYDTDSVSKSVSSKLGVLPKLECIK 176

[Close]

BLAST of Ribonuclease t2 vs. Planmine SMEST
Match: SMESG000048912.1 (SMESG000048912.1)

HSP 1 Score: 126.331 bits (316), Expect = 1.012e-34
Identity = 71/209 (33.97%), Postives = 114/209 (54.55%), Query Frame = 3

Query:   45 VKRVLFILLIVRFAS----SELFDSLMLSIEWPPSYCAQ--GNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNC-DGEPFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIGICFDRSMNLKDC 650
            +K  L I+L +  +S     E +D L++++EWPPS+C      I+C      + GWN+HGLWP    G+D   C +   ++   L  I E+LL  W  L N SK  +FW +EW  HGTC+  +KL   E  YF  T+++KK++ +  +L  +N+ P+N+K Y +    N +K    + P LQC   +   + +L++  CFD  +N+  C
Sbjct:    4 IKYFLLIILSIYTSSIWMKDEDWDYLVMAVEWPPSFCYTFPKKIQC-CDTSHVTGWNLHGLWPSSSKGSDPLCCKNASAYDEKKLKPIMEDLLKEWNPLGNCSK--DFWKHEWKFHGTCASHNKLLHTELQYFNETIQMKKHVDLTNILHQNNVKPDNNKMYKVSDFVNVIKKVYKVEPKLQCTHYKGKIY-ILAMEFCFDTKLNIIMC 208

[Close]

BLAST of Ribonuclease t2 vs. Planmine SMEST
Match: SMESG000048912.1 (SMESG000048912.1)

HSP 1 Score: 123.25 bits (308), Expect = 7.345e-34
Identity = 66/189 (34.92%), Postives = 105/189 (55.56%), Query Frame = 3

Query:   93 ELFDSLMLSIEWPPSYCAQ--GNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNC-DGEPFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIGICFDRSMNLKDC 650
            E +D L++++EWPPS+C      I+C      + GWN+HGLWP    G+D   C +   ++   L  I E+LL  W  L N SK  +FW +EW  HGTC+  +KL   E  YF  T+++KK++ +  +L  +N+ P+N+K Y +    N +K    + P LQC   +   + +L++  CFD  +N+  C
Sbjct:    4 EDWDYLVMAVEWPPSFCYTFPKKIQC-CDTSHVTGWNLHGLWPSSSKGSDPLCCKNASAYDEKKLKPIMEDLLKEWNPLGNCSK--DFWKHEWKFHGTCASHNKLLHTELQYFNETIQMKKHVDLTNILHQNNVKPDNNKMYKVSDFVNVIKKVYKVEPKLQCTHYKGKIY-ILAMEFCFDTKLNIIMC 188

[Close]

BLAST of Ribonuclease t2 vs. Planmine SMEST
Match: SMESG000048912.1 (SMESG000048912.1)

HSP 1 Score: 118.627 bits (296), Expect = 3.752e-32
Identity = 63/182 (34.62%), Postives = 100/182 (54.95%), Query Frame = 3

Query:  114 LSIEWPPSYCAQ--GNIKCHYPIKRIHGWNIHGLWPYKIHGNDTFNC-DGEPFNVNSLISIYEELLLNWANLKNFSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSNHNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIGICFDRSMNLKDC 650
            +++EWPPS+C      I+C      + GWN+HGLWP    G+D   C +   ++   L  I E+LL  W  L N SK  +FW +EW  HGTC+  +KL   E  YF  T+++KK++ +  +L  +N+ P+N+K Y +    N +K    + P LQC   +   + +L++  CFD  +N+  C
Sbjct:    1 MAVEWPPSFCYTFPKKIQC-CDTSHVTGWNLHGLWPSSSKGSDPLCCKNASAYDEKKLKPIMEDLLKEWNPLGNCSK--DFWKHEWKFHGTCASHNKLLHTELQYFNETIQMKKHVDLTNILHQNNVKPDNNKMYKVSDFVNVIKKVYKVEPKLQCTHYKGKIY-ILAMEFCFDTKLNIIMC 178

The following BLAST results are available for this feature:

BLAST of Ribonuclease t2 vs. Ensembl Human
BLAST of Ribonuclease t2 vs. Ensembl Celegans
BLAST of Ribonuclease t2 vs. Ensembl Fly
BLAST of Ribonuclease t2 vs. Ensembl Zebrafish
BLAST of Ribonuclease t2 vs. Ensembl Xenopus
BLAST of Ribonuclease t2 vs. Ensembl Mouse
BLAST of Ribonuclease t2 vs. UniProt/SwissProt
BLAST of Ribonuclease t2 vs. TrEMBL
BLAST of Ribonuclease t2 vs. Ensembl Cavefish
BLAST of Ribonuclease t2 vs. Ensembl Sea Lamprey
BLAST of Ribonuclease t2 vs. Ensembl Yeast
BLAST of Ribonuclease t2 vs. Ensembl Nematostella
BLAST of Ribonuclease t2 vs. Ensembl Medaka
BLAST of Ribonuclease t2 vs. Planmine SMEST

BLAST of Ribonuclease t2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
RNASET2	4.442e-17	27.04	ribonuclease T2 [Source:HGNC Symbol;Acc:HGNC:21686...	[more]
RNASET2	4.468e-17	27.04	ribonuclease T2 [Source:HGNC Symbol;Acc:HGNC:21686...	[more]
RNASET2	4.468e-17	27.04	ribonuclease T2 [Source:HGNC Symbol;Acc:HGNC:21686...	[more]
RNASET2	1.679e-16	28.33	ribonuclease T2 [Source:HGNC Symbol;Acc:HGNC:21686...	[more]
AL159163.1	8.581e-13	25.42	novel protein	[more]

BLAST of Ribonuclease t2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
rnst-2	2.129e-20	29.38	pep chromosome:WBcel235:V:1061595:1067908:1 gene:W...	[more]

BLAST of Ribonuclease t2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1


Match Name	E-value	Identity	Description
RNaseX25	7.748e-23	30.26	gene:FBgn0010406 transcript:FBtr0076752	[more]

BLAST of Ribonuclease t2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
rnaset2	1.592e-21	29.72	ribonuclease T2 [Source:NCBI gene;Acc:791890]	[more]
rnaset2	1.592e-21	29.72	ribonuclease T2 [Source:NCBI gene;Acc:791890]	[more]
rnaset2	1.592e-21	29.72	ribonuclease T2 [Source:NCBI gene;Acc:791890]	[more]
rnaset2	1.592e-21	29.72	ribonuclease T2 [Source:NCBI gene;Acc:791890]	[more]
rnaset2	1.592e-21	29.72	ribonuclease T2 [Source:NCBI gene;Acc:791890]	[more]

BLAST of Ribonuclease t2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
rnaset2	8.146e-10	27.45	ribonuclease T2 [Source:NCBI gene;Acc:780173]	[more]

BLAST of Ribonuclease t2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Rnaset2a	7.272e-18	26.53	ribonuclease T2A [Source:MGI Symbol;Acc:MGI:191544...	[more]
Rnaset2a	7.272e-18	26.53	ribonuclease T2A [Source:MGI Symbol;Acc:MGI:191544...	[more]
Gm49673	7.272e-18	26.53	predicted gene, 49673 [Source:MGI Symbol;Acc:MGI:6...	[more]
Gm49721	7.272e-18	26.53	predicted gene, 49721 [Source:MGI Symbol;Acc:MGI:6...	[more]
Rnaset2b	7.272e-18	26.53	ribonuclease T2B [Source:MGI Symbol;Acc:MGI:370208...	[more]

BLAST of Ribonuclease t2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q7M456\|RNOY_CRAGI	3.924e-29	33.51	Ribonuclease Oy OS=Crassostrea gigas OX=29159 PE=1...	[more]
sp\|P80022\|RNLE_SOLLC	7.958e-25	31.43	Extracellular ribonuclease LE OS=Solanum lycopersi...	[more]
sp\|P42813\|RNS1_ARATH	2.258e-24	31.90	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
sp\|Q7M438\|RNDI_DICDI	4.726e-23	28.77	Ribonuclease DdI OS=Dictyostelium discoideum OX=44...	[more]
sp\|P83618\|RN28_PANGI	6.573e-23	30.22	Ribonuclease-like storage protein OS=Panax ginseng...	[more]

BLAST of Ribonuclease t2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
V4APT3	8.563e-33	34.55	Uncharacterized protein (Fragment) OS=Lottia gigan...	[more]
A0A026WAK0	3.164e-30	34.50	Ribonuclease Oy OS=Ooceraea biroi OX=2015173 GN=DM...	[more]
A0A4Z2DFD7	2.174e-29	31.88	Ribonuclease Oy isoform 2 OS=Schistosoma japonicum...	[more]
A0A4Z2DG04	3.675e-29	32.20	Ribonuclease Oy isoform 1 OS=Schistosoma japonicum...	[more]
A0A564Y0S6	7.734e-29	35.64	Uncharacterized protein (Fragment) OS=Hymenolepis ...	[more]

BLAST of Ribonuclease t2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
rnaset2	8.264e-19	25.54	ribonuclease T2 [Source:NCBI gene;Acc:103036121]	[more]
rnaset2l	5.572e-18	27.05	ribonuclease Oy-like [Source:NCBI gene;Acc:1030229...	[more]

BLAST of Ribonuclease t2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
rnaset2	1.081e-21	28.11	ribonuclease T2 [Source:NCBI gene;Acc:791890]	[more]

BLAST of Ribonuclease t2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
RNY1	5.066e-7	28.29	Vacuolar RNase of the T(2) family; relocalizes to ...	[more]

BLAST of Ribonuclease t2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO48543	8.209e-27	27.48	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO48544	1.636e-21	26.11	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Ribonuclease t2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4


Match Name	E-value	Identity	Description
rnaset2	2.127e-23	29.91	ribonuclease T2 [Source:NCBI gene;Acc:101163650]	[more]
rnaset2	2.127e-23	29.91	ribonuclease T2 [Source:NCBI gene;Acc:101163650]	[more]
rnaset2l	9.352e-19	25.56	ribonuclease Oy [Source:NCBI gene;Acc:101170531]	[more]
rnaset2l	9.352e-19	25.56	ribonuclease Oy [Source:NCBI gene;Acc:101170531]	[more]

BLAST of Ribonuclease t2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000046228.1	1.470e-141	96.04	SMESG000046228.1	[more]
SMESG000016389.1	2.570e-49	42.05	SMESG000016389.1	[more]
SMESG000048912.1	1.012e-34	33.97	SMESG000048912.1	[more]
SMESG000048912.1	7.345e-34	34.92	SMESG000048912.1	[more]
SMESG000048912.1	3.752e-32	34.62	SMESG000048912.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30002004 ID=SMED30002004|Name=Ribonuclease t2|organism=Schmidtea mediterranea sexual|type=transcript|length=850bp
TTTGAATTGAAACTAAAATTATTCTTATGTCTCTTAACATAATGGTGAAA
CGCGTTTTATTTATATTATTAATCGTTCGATTTGCATCAAGTGAATTATT
CGATTCTTTAATGCTGTCAATCGAATGGCCACCCAGTTACTGTGCACAAG
GAAATATCAAGTGCCACTATCCAATCAAAAGGATTCACGGATGGAATATT
CACGGACTATGGCCTTATAAAATACATGGAAACGATACTTTCAATTGTGA
CGGTGAACCTTTCAACGTCAACTCGCTAATTTCAATCTATGAGGAATTAT
TATTAAATTGGGCAAATCTAAAGAATTTTTCCAAACCTGAAGAATTTTGG
GGTTATGAATGGCATAAACACGGAACTTGTTCAATCAAGGACAAATTGTT
CAGTAACGAATTCGGGTATTTTTATCATACCTTACGTCTGAAGAAAAATA
TATCAATGAAAAAACTTCTTTCGAATCACAACATCCTCCCAAACAATGAT
AAAGCTTATAATATTCAACAAATCCACAACGCCGTAAAAGTTGAATTAGG
CATCATACCAATGTTACAATGCTTTCAGAAACAGGATAATGAATGGAATT
TGTTGTCAATAGGTATATGTTTTGACAGAAGCATGAATTTGAAAGATTGT
CAGAATGTGGAGTTACCACAGTCTATGGATTCAATAATTGGAAACTTAAT
TAAGAAGATATTCCTTTATTTGGGAGCCAAAAAAGTAATTACACCAATTA
AAGACAAATGTGAAGGTCATGATTTCAAAATTCCATCTTTTGAATAAATC
TTGAATGTATTAAACAAAATGATACACTAAACAAATTTTTTAACTAATTT

protein sequence of SMED30002004-orf-1

>SMED30002004-orf-1 ID=SMED30002004-orf-1|Name=SMED30002004-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=257bp
MSLNIMVKRVLFILLIVRFASSELFDSLMLSIEWPPSYCAQGNIKCHYPI
KRIHGWNIHGLWPYKIHGNDTFNCDGEPFNVNSLISIYEELLLNWANLKN
FSKPEEFWGYEWHKHGTCSIKDKLFSNEFGYFYHTLRLKKNISMKKLLSN
HNILPNNDKAYNIQQIHNAVKVELGIIPMLQCFQKQDNEWNLLSIGICFD
RSMNLKDCQNVELPQSMDSIIGNLIKKIFLYLGAKKVITPIKDKCEGHDF
KIPSFE*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **cellular component**
Term	Definition
GO:0016020	membrane
GO:0016021	integral component of membrane

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000020	protonephridia
PLANA:0003116	parenchymal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR036430	RNase_T2-like_sf
IPR001568	RNase_T2-like
IPR033130	RNase_T2_His_AS_2
IPR018188	RNase_T2_His_AS_1

Vocabulary: **molecular function**
Term	Definition
GO:0003723	RNA binding
GO:0033897	ribonuclease T2 activity

Vocabulary: **biological process**
Term	Definition
GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 11..181 e-value: 1.6E-35 score: 123.0
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 21..185
IPR036430	Ribonuclease T2-like superfamily	GENE3D	G3DSA:3.90.730.10		coord: 5..192 e-value: 1.3E-41 score: 144.6
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	SSF55895	Ribonuclease Rh-like	coord: 22..184
None	No IPR available	PANTHER	PTHR11240:SF22	RIBONUCLEASE T2	coord: 21..185
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 80..91
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 28..35