GRAM domain-containing protein

Overview
NameGRAM domain-containing protein
Smed IDSMED30002002
Length (bp)1534
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of GRAM domain-containing protein (SMED30002002) t-SNE clustered cells

Violin plots show distribution of expression levels for GRAM domain-containing protein (SMED30002002) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GRAM domain-containing protein (SMED30002002) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GRAM domain-containing protein (SMED30002002) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002002

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30002002SMESG000004908.1 dd_Smed_v4_8544_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30002002SMESG000004908.1 dd_Smed_v4_8544_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30002002SMESG000004908.1 dd_Smed_v4_8544_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30002002SMESG000004908.1 dd_Smed_v4_8544_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30002002SMESG000004908.1 dd_Smed_v4_8544_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30002002SMESG000004908.1 dd_Smed_v4_8544_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002002SMESG000004908.1 Contig37383newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002002SMESG000004908.1 Contig37383uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Human
Match: GRAMD1A (GRAM domain containing 1A [Source:HGNC Symbol;Acc:HGNC:29305])

HSP 1 Score: 92.8189 bits (229), Expect = 6.307e-21
Identity = 52/126 (41.27%), Postives = 74/126 (58.73%), Query Frame = 1 
Query:  355 STASNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTNREDERFP 729
            S  S  YK +N   FRK+F    E+E L+  +SCAL  EIL QG LY S+N+ CF+S I   +  + I ++++  + KEKTA + PNAI I TE  +H F+SF  R+  F  +  +W N   E+ P
Sbjct:   80 SMLSPTYKQRNE-DFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQNALLEKDP 204
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Human
Match: GRAMD1B (GRAM domain containing 1B [Source:HGNC Symbol;Acc:HGNC:29214])

HSP 1 Score: 89.3521 bits (220), Expect = 2.925e-20
Identity = 45/116 (38.79%), Postives = 70/116 (60.34%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRK+F    ++E L+  +SCAL  +IL QG LY S+N+ CF+S I   +  + + ++ I  +TKEKTA + PNAI + T+  +H F+SF  R+  +  +  +W N
Sbjct:   48 SPTYKQRNE-DFRKLFKQLPDTERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQN 162
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Human
Match: GRAMD1A (GRAM domain containing 1A [Source:HGNC Symbol;Acc:HGNC:29305])

HSP 1 Score: 92.8189 bits (229), Expect = 3.346e-19
Identity = 50/119 (42.02%), Postives = 71/119 (59.66%), Query Frame = 1 
Query:  355 STASNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  S  YK +N   FRK+F    E+E L+  +SCAL  EIL QG LY S+N+ CF+S I   +  + I ++++  + KEKTA + PNAI I TE  +H F+SF  R+  F  +  +W N
Sbjct:  167 SMLSPTYKQRNE-DFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQN 284
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Human
Match: GRAMD1A (GRAM domain containing 1A [Source:HGNC Symbol;Acc:HGNC:29305])

HSP 1 Score: 92.4337 bits (228), Expect = 4.458e-19
Identity = 50/119 (42.02%), Postives = 71/119 (59.66%), Query Frame = 1 
Query:  355 STASNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  S  YK +N   FRK+F    E+E L+  +SCAL  EIL QG LY S+N+ CF+S I   +  + I ++++  + KEKTA + PNAI I TE  +H F+SF  R+  F  +  +W N
Sbjct:   80 SMLSPTYKQRNE-DFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQN 197
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Human
Match: GRAMD1A (GRAM domain containing 1A [Source:HGNC Symbol;Acc:HGNC:29305])

HSP 1 Score: 91.6633 bits (226), Expect = 6.441e-19
Identity = 49/116 (42.24%), Postives = 70/116 (60.34%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRK+F    E+E L+  +SCAL  EIL QG LY S+N+ CF+S I   +  + I ++++  + KEKTA + PNAI I TE  +H F+SF  R+  F  +  +W N
Sbjct:   76 SPTYKQRNE-DFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQN 190
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Celegans
Match: ZC328.3 (pep chromosome:WBcel235:I:6409050:6417010:-1 gene:WBGene00022593.1 transcript:ZC328.3a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZC328.3)

HSP 1 Score: 69.707 bits (169), Expect = 2.226e-12
Identity = 43/138 (31.16%), Postives = 78/138 (56.52%), Query Frame = 1 
Query:  388 NFKFRKIFD----FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAI-RTEHNRHLFSSFVHREIAFRCLNEVWTN--REDERFPPQ---WNLGFGEETKDN 771
            N +F+KIF      EE++  + ++SCA   EIL QG ++ SQ   CF++ I   +  ++I +++I  + K K A IFPN+I I R    ++ F+SF++R+ +++ L         EDE+   +   W++ +  E K++
Sbjct:   73 NLQFKKIFVDKGLIEENDQFLASYSCAYQREILAQGRMFISQFNVCFYANIIGWETTLVIPMKEISLVKKMKAAFIFPNSIQIERDTKEKYFFASFINRDKSYQVLTTAHQKVAGEDEKAMTREEVWDMVYNSEDKNS 210
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Fly
Match: GramD1B (gene:FBgn0085423 transcript:FBtr0332409)

HSP 1 Score: 90.8929 bits (224), Expect = 8.284e-19
Identity = 57/148 (38.51%), Postives = 85/148 (57.43%), Query Frame = 1 
Query:  373 YKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTNR-EDERFPPQ--W---NLGFGEE----TKDNSLID 783
            YKS+    F+K+F D    E L+  +SCAL  +IL QG LY SQN+ CF + I + +  V I  + +  ITKEKTA + PNAI+I +  +++ F++F  R+ +F  L  VW N   +++F PQ  W   +  +G+E    T D   ID
Sbjct:  308 YKSRAE-DFKKLFKDVPNDERLIVDYSCALQRDILVQGRLYVSQNYVCFHANIFSWETHVSIKWKDVTAITKEKTALVIPNAISISSGKDKYFFATFTSRDKSFLMLFRVWQNTLMNKQFSPQEIWQHVHTCYGDELGLTTDDEDYID 454
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Fly
Match: GramD1B (gene:FBgn0085423 transcript:FBtr0112641)

HSP 1 Score: 90.8929 bits (224), Expect = 8.744e-19
Identity = 57/148 (38.51%), Postives = 85/148 (57.43%), Query Frame = 1 
Query:  373 YKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTNR-EDERFPPQ--W---NLGFGEE----TKDNSLID 783
            YKS+    F+K+F D    E L+  +SCAL  +IL QG LY SQN+ CF + I + +  V I  + +  ITKEKTA + PNAI+I +  +++ F++F  R+ +F  L  VW N   +++F PQ  W   +  +G+E    T D   ID
Sbjct:  332 YKSRAE-DFKKLFKDVPNDERLIVDYSCALQRDILVQGRLYVSQNYVCFHANIFSWETHVSIKWKDVTAITKEKTALVIPNAISISSGKDKYFFATFTSRDKSFLMLFRVWQNTLMNKQFSPQEIWQHVHTCYGDELGLTTDDEDYID 478
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Fly
Match: GramD1B (gene:FBgn0085423 transcript:FBtr0112638)

HSP 1 Score: 90.8929 bits (224), Expect = 8.827e-19
Identity = 57/148 (38.51%), Postives = 85/148 (57.43%), Query Frame = 1 
Query:  373 YKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTNR-EDERFPPQ--W---NLGFGEE----TKDNSLID 783
            YKS+    F+K+F D    E L+  +SCAL  +IL QG LY SQN+ CF + I + +  V I  + +  ITKEKTA + PNAI+I +  +++ F++F  R+ +F  L  VW N   +++F PQ  W   +  +G+E    T D   ID
Sbjct:  406 YKSRAE-DFKKLFKDVPNDERLIVDYSCALQRDILVQGRLYVSQNYVCFHANIFSWETHVSIKWKDVTAITKEKTALVIPNAISISSGKDKYFFATFTSRDKSFLMLFRVWQNTLMNKQFSPQEIWQHVHTCYGDELGLTTDDEDYID 552
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Fly
Match: GramD1B (gene:FBgn0085423 transcript:FBtr0112639)

HSP 1 Score: 90.8929 bits (224), Expect = 8.920e-19
Identity = 57/148 (38.51%), Postives = 85/148 (57.43%), Query Frame = 1 
Query:  373 YKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTNR-EDERFPPQ--W---NLGFGEE----TKDNSLID 783
            YKS+    F+K+F D    E L+  +SCAL  +IL QG LY SQN+ CF + I + +  V I  + +  ITKEKTA + PNAI+I +  +++ F++F  R+ +F  L  VW N   +++F PQ  W   +  +G+E    T D   ID
Sbjct:  332 YKSRAE-DFKKLFKDVPNDERLIVDYSCALQRDILVQGRLYVSQNYVCFHANIFSWETHVSIKWKDVTAITKEKTALVIPNAISISSGKDKYFFATFTSRDKSFLMLFRVWQNTLMNKQFSPQEIWQHVHTCYGDELGLTTDDEDYID 478
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Fly
Match: GramD1B (gene:FBgn0085423 transcript:FBtr0306333)

HSP 1 Score: 90.5077 bits (223), Expect = 9.835e-19
Identity = 57/148 (38.51%), Postives = 85/148 (57.43%), Query Frame = 1 
Query:  373 YKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTNR-EDERFPPQ--W---NLGFGEE----TKDNSLID 783
            YKS+    F+K+F D    E L+  +SCAL  +IL QG LY SQN+ CF + I + +  V I  + +  ITKEKTA + PNAI+I +  +++ F++F  R+ +F  L  VW N   +++F PQ  W   +  +G+E    T D   ID
Sbjct:  342 YKSRAE-DFKKLFKDVPNDERLIVDYSCALQRDILVQGRLYVSQNYVCFHANIFSWETHVSIKWKDVTAITKEKTALVIPNAISISSGKDKYFFATFTSRDKSFLMLFRVWQNTLMNKQFSPQEIWQHVHTCYGDELGLTTDDEDYID 488
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Zebrafish
Match: gramd1a (GRAM domain containing 1A [Source:ZFIN;Acc:ZDB-GENE-060503-688])

HSP 1 Score: 93.5893 bits (231), Expect = 1.986e-21
Identity = 47/116 (40.52%), Postives = 72/116 (62.07%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRK+F    ++E L+  +SCAL  +IL QG LY S+N+ CF+S I   +  + I ++ +  +TKEKTA + PNAI I TE+ +H F+SF  R+ ++  +  +W N
Sbjct:   72 SPTYKQRNE-DFRKLFKKLPDTERLIVDYSCALQKDILLQGRLYLSENWLCFYSNIFRWETTITILLKDVTNLTKEKTAKLIPNAIQISTENEKHFFTSFGARDRSYMMIFRLWQN 186
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Zebrafish
Match: gramd1a (GRAM domain containing 1A [Source:ZFIN;Acc:ZDB-GENE-060503-688])

HSP 1 Score: 95.5153 bits (236), Expect = 2.555e-20
Identity = 47/116 (40.52%), Postives = 72/116 (62.07%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRK+F    ++E L+  +SCAL  +IL QG LY S+N+ CF+S I   +  + I ++ +  +TKEKTA + PNAI I TE+ +H F+SF  R+ ++  +  +W N
Sbjct:   79 SPTYKQRNE-DFRKLFKKLPDTERLIVDYSCALQKDILLQGRLYLSENWLCFYSNIFRWETTITILLKDVTNLTKEKTAKLIPNAIQISTENEKHFFTSFGARDRSYMMIFRLWQN 193
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Zebrafish
Match: AL953903.4 (GRAM domain containing 1Ba [Source:NCBI gene;Acc:568698])

HSP 1 Score: 92.0485 bits (227), Expect = 3.897e-19
Identity = 45/125 (36.00%), Postives = 77/125 (61.60%), Query Frame = 1 
Query:  337 GQSMEISTASNLYKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            G++ +++  S  YK +N   FRK+F    ++E L+  +SCAL  +IL QG LY S+N+ CF+S I   +  + + ++ +  +TKEKTA + PNAI + T++ +H F+SF  R+  +  +  +W N
Sbjct:   80 GKNSKVNVLSPTYKQRNE-DFRKLFKQLPDTERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDVCSMTKEKTARLIPNAIQLCTDNEKHFFTSFGARDRTYMMMFRLWQN 203
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Zebrafish
Match: gramd1bb (GRAM domain containing 1Bb [Source:ZFIN;Acc:ZDB-GENE-140106-106])

HSP 1 Score: 89.7373 bits (221), Expect = 2.351e-18
Identity = 45/116 (38.79%), Postives = 70/116 (60.34%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRK+F    ++E L+  +SCAL  +IL QG LY S+N+ CF+S I   +  + + ++ I  +TKEKTA + PNAI + T+  +H F+SF  R+  +  +  +W N
Sbjct:  242 SPTYKQRNE-DFRKLFKQLPDTERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDTEKHFFTSFGARDRTYMMMFRLWQN 356
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Zebrafish
Match: gramd1bb (GRAM domain containing 1Bb [Source:ZFIN;Acc:ZDB-GENE-140106-106])

HSP 1 Score: 89.3521 bits (220), Expect = 2.628e-18
Identity = 45/116 (38.79%), Postives = 70/116 (60.34%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRK+F    ++E L+  +SCAL  +IL QG LY S+N+ CF+S I   +  + + ++ I  +TKEKTA + PNAI + T+  +H F+SF  R+  +  +  +W N
Sbjct:   85 SPTYKQRNE-DFRKLFKQLPDTERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDTEKHFFTSFGARDRTYMMMFRLWQN 199
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Xenopus
Match: GRAMD1B (GRAM domain containing 1B [Source:NCBI gene;Acc:100497114])

HSP 1 Score: 90.8929 bits (224), Expect = 1.306e-18
Identity = 47/116 (40.52%), Postives = 70/116 (60.34%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRK+F    +SE L+  +SCAL  +IL QG LY S+N+ CF+S I   +  + + ++ I  +TKEKTA + PNAI + TE  +H F+SF  R+  +  +  +W N
Sbjct:   81 SPTYKQRNE-DFRKLFKQLPDSERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQLCTETEKHFFTSFGARDRTYMMMFRLWQN 195
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Xenopus
Match: GRAMD1B (GRAM domain containing 1B [Source:NCBI gene;Acc:100497114])

HSP 1 Score: 90.5077 bits (223), Expect = 1.433e-18
Identity = 47/116 (40.52%), Postives = 70/116 (60.34%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRK+F    +SE L+  +SCAL  +IL QG LY S+N+ CF+S I   +  + + ++ I  +TKEKTA + PNAI + TE  +H F+SF  R+  +  +  +W N
Sbjct:   84 SPTYKQRNE-DFRKLFKQLPDSERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQLCTETEKHFFTSFGARDRTYMMMFRLWQN 198
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Xenopus
Match: GRAMD1B (GRAM domain containing 1B [Source:NCBI gene;Acc:100497114])

HSP 1 Score: 90.5077 bits (223), Expect = 1.468e-18
Identity = 47/116 (40.52%), Postives = 70/116 (60.34%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRK+F    +SE L+  +SCAL  +IL QG LY S+N+ CF+S I   +  + + ++ I  +TKEKTA + PNAI + TE  +H F+SF  R+  +  +  +W N
Sbjct:   84 SPTYKQRNE-DFRKLFKQLPDSERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQLCTETEKHFFTSFGARDRTYMMMFRLWQN 198
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Xenopus
Match: GRAMD1B (GRAM domain containing 1B [Source:NCBI gene;Acc:100497114])

HSP 1 Score: 90.5077 bits (223), Expect = 1.519e-18
Identity = 47/116 (40.52%), Postives = 70/116 (60.34%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRK+F    +SE L+  +SCAL  +IL QG LY S+N+ CF+S I   +  + + ++ I  +TKEKTA + PNAI + TE  +H F+SF  R+  +  +  +W N
Sbjct:  208 SPTYKQRNE-DFRKLFKQLPDSERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQLCTETEKHFFTSFGARDRTYMMMFRLWQN 322
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Xenopus
Match: GRAMD1B (GRAM domain containing 1B [Source:NCBI gene;Acc:100497114])

HSP 1 Score: 90.5077 bits (223), Expect = 1.533e-18
Identity = 47/116 (40.52%), Postives = 70/116 (60.34%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRK+F    +SE L+  +SCAL  +IL QG LY S+N+ CF+S I   +  + + ++ I  +TKEKTA + PNAI + TE  +H F+SF  R+  +  +  +W N
Sbjct:   64 SPTYKQRNE-DFRKLFKQLPDSERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQLCTETEKHFFTSFGARDRTYMMMFRLWQN 178
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Mouse
Match: Gramd1a (GRAM domain containing 1A [Source:MGI Symbol;Acc:MGI:105490])

HSP 1 Score: 92.0485 bits (227), Expect = 2.026e-21
Identity = 61/155 (39.35%), Postives = 87/155 (56.13%), Query Frame = 1 
Query:  247 NSESPTVSGSNIILSSATISRPAFSWCSKCGQSMEISTASNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            +SE   VSG+   LS+ ++    F   SK  QS   S     YK +N   FRK+F    E+E L+  +SCAL  EIL QG LY S+N+ CF+S I   +  + I ++++  + KEKTA + PNAI I TE  +H F+SF  R+  F  +  +W N
Sbjct:    9 SSEKSGVSGT---LSTQSLGSRNFIRNSKKMQSW-YSMLCPTYKQRNE-DFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQN 158
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Mouse
Match: Gramd1a (GRAM domain containing 1A [Source:MGI Symbol;Acc:MGI:105490])

HSP 1 Score: 92.8189 bits (229), Expect = 1.440e-19
Identity = 48/113 (42.48%), Postives = 69/113 (61.06%), Query Frame = 1 
Query:  373 YKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            YK +N   FRK+F    E+E L+  +SCAL  EIL QG LY S+N+ CF+S I   +  + I ++++  + KEKTA + PNAI I TE  +H F+SF  R+  F  +  +W N
Sbjct:   88 YKQRNE-DFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQN 199
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Mouse
Match: Gramd1a (GRAM domain containing 1A [Source:MGI Symbol;Acc:MGI:105490])

HSP 1 Score: 92.4337 bits (228), Expect = 2.557e-19
Identity = 48/113 (42.48%), Postives = 69/113 (61.06%), Query Frame = 1 
Query:  373 YKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            YK +N   FRK+F    E+E L+  +SCAL  EIL QG LY S+N+ CF+S I   +  + I ++++  + KEKTA + PNAI I TE  +H F+SF  R+  F  +  +W N
Sbjct:   88 YKQRNE-DFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQN 199
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Mouse
Match: Gramd1a (GRAM domain containing 1A [Source:MGI Symbol;Acc:MGI:105490])

HSP 1 Score: 92.4337 bits (228), Expect = 2.769e-19
Identity = 60/161 (37.27%), Postives = 88/161 (54.66%), Query Frame = 1 
Query:  229 SMTQSINSESPTVSGSNIILSSATISRPAFSWCSKCGQSMEISTASNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            +M +  +  S   SG +  LS+ ++    F   SK  QS   S     YK +N   FRK+F    E+E L+  +SCAL  EIL QG LY S+N+ CF+S I   +  + I ++++  + KEKTA + PNAI I TE  +H F+SF  R+  F  +  +W N
Sbjct:   41 TMVEKGSDSSSEKSGVSGTLSTQSLGSRNFIRNSKKMQSW-YSMLCPTYKQRNE-DFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQN 199
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Mouse
Match: Gramd1b (GRAM domain containing 1B [Source:MGI Symbol;Acc:MGI:1925037])

HSP 1 Score: 89.7373 bits (221), Expect = 2.216e-18
Identity = 45/116 (38.79%), Postives = 70/116 (60.34%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRK+F    ++E L+  +SCAL  +IL QG LY S+N+ CF+S I   +  + + ++ I  +TKEKTA + PNAI + T+  +H F+SF  R+  +  +  +W N
Sbjct:  232 SPTYKQRNE-DFRKLFKQLPDTERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQN 346
[Close]
BLAST of GRAM domain-containing protein vs. UniProt/SwissProt
Match: sp|Q3KR56|ASTRA_RAT (Protein Aster-A OS=Rattus norvegicus OX=10116 GN=Gramd1a PE=2 SV=2)

HSP 1 Score: 92.4337 bits (228), Expect = 1.922e-18
Identity = 60/161 (37.27%), Postives = 88/161 (54.66%), Query Frame = 1 
Query:  229 SMTQSINSESPTVSGSNIILSSATISRPAFSWCSKCGQSMEISTASNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            +M +  +  S   SG +  LS+ ++    F   SK  QS   S     YK +N   FRK+F    E+E L+  +SCAL  EIL QG LY S+N+ CF+S I   +  + I ++++  + KEKTA + PNAI I TE  +H F+SF  R+  F  +  +W N
Sbjct:   41 TMVEKGSDSSSEKSGVSGTLSTQSLGSRNFIRNSKKMQSW-YSMLCPTYKQRNE-DFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQN 199
[Close]
BLAST of GRAM domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8VEF1|ASTRA_MOUSE (Protein Aster-A OS=Mus musculus OX=10090 GN=Gramd1a PE=1 SV=2)

HSP 1 Score: 92.4337 bits (228), Expect = 1.937e-18
Identity = 60/161 (37.27%), Postives = 88/161 (54.66%), Query Frame = 1 
Query:  229 SMTQSINSESPTVSGSNIILSSATISRPAFSWCSKCGQSMEISTASNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            +M +  +  S   SG +  LS+ ++    F   SK  QS   S     YK +N   FRK+F    E+E L+  +SCAL  EIL QG LY S+N+ CF+S I   +  + I ++++  + KEKTA + PNAI I TE  +H F+SF  R+  F  +  +W N
Sbjct:   41 TMVEKGSDSSSEKSGVSGTLSTQSLGSRNFIRNSKKMQSW-YSMLCPTYKQRNE-DFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQN 199
[Close]
BLAST of GRAM domain-containing protein vs. UniProt/SwissProt
Match: sp|Q96CP6|ASTRA_HUMAN (Protein Aster-A OS=Homo sapiens OX=9606 GN=GRAMD1A PE=1 SV=2)

HSP 1 Score: 92.4337 bits (228), Expect = 2.141e-18
Identity = 50/119 (42.02%), Postives = 71/119 (59.66%), Query Frame = 1 
Query:  355 STASNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  S  YK +N   FRK+F    E+E L+  +SCAL  EIL QG LY S+N+ CF+S I   +  + I ++++  + KEKTA + PNAI I TE  +H F+SF  R+  F  +  +W N
Sbjct:   80 SMLSPTYKQRNE-DFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQN 197
[Close]
BLAST of GRAM domain-containing protein vs. UniProt/SwissProt
Match: sp|Q80TI0|ASTRB_MOUSE (Protein Aster-B OS=Mus musculus OX=10090 GN=Gramd1b PE=1 SV=2)

HSP 1 Score: 89.3521 bits (220), Expect = 1.922e-17
Identity = 45/116 (38.79%), Postives = 70/116 (60.34%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRK+F    ++E L+  +SCAL  +IL QG LY S+N+ CF+S I   +  + + ++ I  +TKEKTA + PNAI + T+  +H F+SF  R+  +  +  +W N
Sbjct:   88 SPTYKQRNE-DFRKLFKQLPDTERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQN 202
[Close]
BLAST of GRAM domain-containing protein vs. UniProt/SwissProt
Match: sp|Q3KR37|ASTRB_HUMAN (Protein Aster-B OS=Homo sapiens OX=9606 GN=GRAMD1B PE=1 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.922e-17
Identity = 53/153 (34.64%), Postives = 85/153 (55.56%), Query Frame = 1 
Query:  253 ESPTVSGSNIILSSATISRPAFSWCSKCGQSMEISTASNLYKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            +SP+     +  S ++ S  +    SK  QS   +  S  YK +N   FRK+F    ++E L+  +SCAL  +IL QG LY S+N+ CF+S I   +  + + ++ I  +TKEKTA + PNAI + T+  +H F+SF  R+  +  +  +W N
Sbjct:   52 KSPSTPEQGVQRSCSSQSGRSGGKNSKKSQSW-YNVLSPTYKQRNE-DFRKLFKQLPDTERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQN 202
[Close]
BLAST of GRAM domain-containing protein vs. TrEMBL
Match: A0A1I8G575 (GRAM domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 1.380e-27
Identity = 56/103 (54.37%), Postives = 74/103 (71.84%), Query Frame = 1 
Query:  394 KFRKIFDFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVW 702
            KF ++FD   +EVL++TF CAL  EIL QGT Y S NFFCF++R+ANK+K + I I  + EIT+EKTA  FPNAI IRTE +R++F S + RE   + L E+W
Sbjct:    7 KFHRLFDIATTEVLIRTFPCALKAEILLQGTFYVSANFFCFYARLANKRKTIKIPIRTVCEITREKTAIFFPNAIGIRTEDSRYVFGSILSRENTLKLLLELW 109
[Close]
BLAST of GRAM domain-containing protein vs. TrEMBL
Match: A0A1I8J0U7 (GRAM domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 1.548e-27
Identity = 56/103 (54.37%), Postives = 74/103 (71.84%), Query Frame = 1 
Query:  394 KFRKIFDFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVW 702
            KF ++FD   +EVL++TF CAL  EIL QGT Y S NFFCF++R+ANK+K + I I  + EIT+EKTA  FPNAI IRTE +R++F S + RE   + L E+W
Sbjct:    7 KFHRLFDIATTEVLIRTFPCALKAEILLQGTFYVSANFFCFYARLANKRKTIKIPIRTVCEITREKTAIFFPNAIGIRTEDSRYVFGSILSRENTLKLLLELW 109
[Close]
BLAST of GRAM domain-containing protein vs. TrEMBL
Match: A0A267G4X2 (GRAM domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009334g2 PE=4 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 3.008e-27
Identity = 56/103 (54.37%), Postives = 74/103 (71.84%), Query Frame = 1 
Query:  394 KFRKIFDFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVW 702
            KF ++FD   +EVL++TF CAL  EIL QGT Y S NFFCF++R+ANK+K + I I  + EIT+EKTA  FPNAI IRTE +R++F S + RE   + L E+W
Sbjct:   78 KFHRLFDIATTEVLIRTFPCALKAEILLQGTFYVSANFFCFYARLANKRKTIKIPIRTVCEITREKTAIFFPNAIGIRTEDSRYVFGSILSRENTLKLLLELW 180
[Close]
BLAST of GRAM domain-containing protein vs. TrEMBL
Match: A0A267GQY9 (GRAM domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009334g1 PE=4 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 3.142e-27
Identity = 56/103 (54.37%), Postives = 74/103 (71.84%), Query Frame = 1 
Query:  394 KFRKIFDFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVW 702
            KF ++FD   +EVL++TF CAL  EIL QGT Y S NFFCF++R+ANK+K + I I  + EIT+EKTA  FPNAI IRTE +R++F S + RE   + L E+W
Sbjct:   78 KFHRLFDIATTEVLIRTFPCALKAEILLQGTFYVSANFFCFYARLANKRKTIKIPIRTVCEITREKTAIFFPNAIGIRTEDSRYVFGSILSRENTLKLLLELW 180
[Close]
BLAST of GRAM domain-containing protein vs. TrEMBL
Match: A0A1Q3G1V5 (Protein in glucosyltransferase OS=Culex tarsalis OX=7177 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 2.674e-20
Identity = 47/110 (42.73%), Postives = 70/110 (63.64%), Query Frame = 1 
Query:  373 YKSKNNFKFRKIFDFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVW 702
            + S    KF + F   E E ++  FSCALV +IL QG LY +QN+F F+S +     K++I    +++I+KEKTA +FPNA+ + T  +RH+F SF+ RE A+R +  VW
Sbjct:  103 FNSSRQKKFSRHFTQVEDEKVLNHFSCALVSDILLQGHLYITQNYFAFYSNVFGYVTKLLIPTVSVIKISKEKTAKMFPNAVGVTTSDDRHVFGSFMSREAAYRLMCSVW 212
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000038267.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001937.1:53880:60741:1 gene:ENSAMXG00000036136.1 transcript:ENSAMXT00000038267.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 92.0485 bits (227), Expect = 4.683e-21
Identity = 47/116 (40.52%), Postives = 71/116 (61.21%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRKIF    ++E L+  +SCAL  +IL QG LY S+N+ CF+S I   +  + I ++ +  +TKEKTA + PNAI I T+  +H F+SF  R+ ++  +  +W N
Sbjct:   72 SPTYKQRNE-DFRKIFKKLPDTERLIVDYSCALQKDILLQGRLYLSENWICFYSNIFRWETTITILLKDVTSLTKEKTAKLIPNAIQICTDQEKHFFTSFGARDRSYMMIFRLWQN 186
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000012808.2 (GRAM domain-containing protein 2B-like [Source:NCBI gene;Acc:103035606])

HSP 1 Score: 89.7373 bits (221), Expect = 5.374e-19
Identity = 42/114 (36.84%), Postives = 71/114 (62.28%), Query Frame = 1 
Query:  382 KNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTNREDE 720
            ++N ++ K+F D  E+E L ++++CAL  +IL+QG L+ S+N+ CF S++  K  K+ I +  +  I K KTA + PNA+ I T   RH+F SF+ R+  ++ L  V  +  D+
Sbjct:  109 RSNSQYHKVFKDISENEHLKQSYTCALQKDILYQGRLFVSENWICFHSKVFGKDTKISIPVPSVTVIKKTKTAILVPNALVIATAQERHVFVSFLSRDTTYKVLMSVCIHLHDK 222
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000057118.1 (GRAM domain-containing protein 2B-like [Source:NCBI gene;Acc:103035606])

HSP 1 Score: 89.7373 bits (221), Expect = 6.624e-19
Identity = 42/114 (36.84%), Postives = 71/114 (62.28%), Query Frame = 1 
Query:  382 KNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTNREDE 720
            ++N ++ K+F D  E+E L ++++CAL  +IL+QG L+ S+N+ CF S++  K  K+ I +  +  I K KTA + PNA+ I T   RH+F SF+ R+  ++ L  V  +  D+
Sbjct:  109 RSNSQYHKVFKDISENEHLKQSYTCALQKDILYQGRLFVSENWICFHSKVFGKDTKISIPVPSVTVIKKTKTAILVPNALVIATAQERHVFVSFLSRDTTYKVLMSVCIHLHDK 222
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000042002.1 (GRAM domain-containing protein 2B-like [Source:NCBI gene;Acc:103035606])

HSP 1 Score: 89.3521 bits (220), Expect = 6.884e-19
Identity = 42/114 (36.84%), Postives = 71/114 (62.28%), Query Frame = 1 
Query:  382 KNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTNREDE 720
            ++N ++ K+F D  E+E L ++++CAL  +IL+QG L+ S+N+ CF S++  K  K+ I +  +  I K KTA + PNA+ I T   RH+F SF+ R+  ++ L  V  +  D+
Sbjct:  109 RSNSQYHKVFKDISENEHLKQSYTCALQKDILYQGRLFVSENWICFHSKVFGKDTKISIPVPSVTVIKKTKTAILVPNALVIATAQERHVFVSFLSRDTTYKVLMSVCIHLHDK 222
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Cavefish
Match: gramd2aa (GRAM domain-containing protein 2A [Source:NCBI gene;Acc:103031102])

HSP 1 Score: 88.1965 bits (217), Expect = 9.666e-19
Identity = 45/130 (34.62%), Postives = 80/130 (61.54%), Query Frame = 1 
Query:  334 CGQSM-EISTASNLYK--SKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHN-RHLFSSFVHREIAFRCLNEVWTN 708
            CGQ +  + +A++  +  SK N ++ K+F    + E+L+K +SCAL+ +IL QG LY S+N+ CF++ +  K  KV I +  +  + K KTAG+ PN +AI T+ + +++F S + R+  +  L  + T+
Sbjct:   21 CGQELISVPSAASPSQTVSKYNSQYHKLFQTVPKEEILMKVYSCALLRDILLQGRLYISRNWLCFYANLFGKDIKVAIPVVSVRLVKKHKTAGLVPNGLAITTDSSQKYVFVSLLSRDSVYDVLRRICTH 150
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Sea Lamprey
Match: gramd1bb (GRAM domain containing 1Bb [Source:ZFIN;Acc:ZDB-GENE-140106-106])

HSP 1 Score: 94.3597 bits (233), Expect = 8.796e-21
Identity = 50/116 (43.10%), Postives = 72/116 (62.07%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRK+F    ESE L+  +SCAL  +IL QG LY S+N+ CF+S I   +  + I +++I  +TKEKTA + PNAI I TE+++H F+S   R+  F  +  +W N
Sbjct:   11 SPTYKQRNE-DFRKLFKQLPESERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTIRLKEITAMTKEKTARLIPNAIQICTENDKHFFTSLGARDRTFMMMFRLWQN 125
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Sea Lamprey
Match: GRAMD1A (GRAM domain containing 1A [Source:HGNC Symbol;Acc:HGNC:29305])

HSP 1 Score: 84.7297 bits (208), Expect = 5.833e-18
Identity = 45/116 (38.79%), Postives = 70/116 (60.34%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  Y+ +N+  FR+IF    E+E LV  +SCAL  +IL  G LY S+ + CF S I   +  + I +++I  +TKE+TA + PNAI + T++ ++ FSS   R+ AF  +  +W N
Sbjct:    1 SAPYRQRND-DFRRIFKQLPENEHLVVDYSCALQRDILVHGRLYLSEGWLCFHSNIFRWETLLTIQMKEITAVTKERTARVIPNAIQVCTQNEKYFFSSLGARDRAFLMIFRLWQN 115
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003678.1 (pep scaffold:Pmarinus_7.0:GL477104:103280:105081:-1 gene:ENSPMAG00000003360.1 transcript:ENSPMAT00000003678.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 72.7886 bits (177), Expect = 6.014e-16
Identity = 34/91 (37.36%), Postives = 56/91 (61.54%), Query Frame = 1 
Query:  442 TFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTNRE 714
            TF+CAL  +++FQG ++ S+N+ CFF+    K  KV I +  +  I K+++A + PNAI I +   +H+F SF+ R+ A++ L  V    E
Sbjct:   25 TFNCALQRDLIFQGRMFVSENWICFFANFFGKDVKVAIPVLTVKAIKKQRSALLLPNAIGITSACEKHMFISFLARDAAYKALLSVCKQAE 115
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Sea Lamprey
Match: TBC1D9 (TBC1 domain family member 9 [Source:HGNC Symbol;Acc:HGNC:21710])

HSP 1 Score: 55.8398 bits (133), Expect = 1.582e-8
Identity = 32/91 (35.16%), Postives = 50/91 (54.95%), Query Frame = 1 
Query:  394 KFRKIFDFEESEVLVKTFSCAL-VDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFV 663
            +FRK F    +E+LV   SC+     +  QG LY S N  CF+S +  K+ K+ I    ++ + K+ +A  FP A+ + T H +H FS F+
Sbjct:   67 RFRKFFAAPSTELLVVAVSCSYWKGRMPRQGWLYLSVNHACFYSVLLGKEAKLAIPWVDVVRLEKKGSAP-FPEAVVLTTRHVQHNFSMFL 156
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006389.1 (pep scaffold:Pmarinus_7.0:GL481951:143257:161898:-1 gene:ENSPMAG00000005741.1 transcript:ENSPMAT00000006389.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.9138 bits (128), Expect = 8.146e-8
Identity = 30/103 (29.13%), Postives = 50/103 (48.54%), Query Frame = 1 
Query:  394 KFRKIFDFEESEVLVKTFSCALV-DEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEV 699
            KFRK+F   E E LV  +SC+     +  QG LY S N  CF+S +  K+ K+++    +  + +  T  + P ++ + T      FS F+     FR +  +
Sbjct:   72 KFRKLFGMPEEERLVNYYSCSYWKGRVPRQGWLYLSVNHVCFYSLLLGKEAKLVVRWADVTCLERTATV-VLPESVRVATRDGDLFFSMFLSVGETFRLMERL 173
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Yeast
Match: YSP2 (Sterol-binding protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; contains two StART-like domains that bind sterols and a GRAM domain; co-localizes to puncta in the cortical ER with Sip3p; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes [Source:SGD;Acc:S000002734])

HSP 1 Score: 86.6557 bits (213), Expect = 3.195e-18
Identity = 44/110 (40.00%), Postives = 63/110 (57.27%), Query Frame = 1 
Query:  382 KNNFKFRKIF---DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVW 702
            K N +F  +F   D   +E L+   SCAL  +IL QG +Y S     FFS I      V I  ++I++I K+ TAGIFPN I I T H +++F+SF+ R+  F  + +VW
Sbjct:  644 KKNSEFHTLFKDCDINPNEKLIVDHSCALSRDILLQGRMYISDAHIGFFSNILGWVSTVFIPFKEIVQIEKKTTAGIFPNGIVIDTLHTKYIFASFMSRDATFDLITDVW 753
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Yeast
Match: YFL042C_mRNA (Putative sterol transfer protein; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; has both GRAM and StART-like (VASt) domains; localizes to membrane contact sites throughout the cell, including nucleus-vacuole junctions and ER-mitochondrial contact sites [Source:SGD;Acc:S000001852])

HSP 1 Score: 81.2629 bits (199), Expect = 9.906e-17
Identity = 36/91 (39.56%), Postives = 56/91 (61.54%), Query Frame = 1 
Query:  433 LVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWT 705
            L+  F+C L  E+L+QG LY ++   CF S +     KV+IA E +  + K   AG+FP+AI+I T+  + LF+ F+ R+ AF  + EVW+
Sbjct:  213 LLDDFNCGLNRELLYQGKLYITETHLCFNSNVLGWIAKVLIAFEDVTFMEKTSAAGLFPSAISIETKMGKTLFNGFISRDAAFGLMKEVWS 303
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Yeast
Match: LAM4 (Sterol-binding protein that localizes to puncta in the cortical ER; sterol binding occurs via two StART-like domains; one of six StART-like domain-containing proteins in yeast that may be involved in intracellular sterol transfer between membranes; conserved across eukaryotes; has both GRAM and StART-like (VASt) domains; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies [Source:SGD;Acc:S000001122])

HSP 1 Score: 81.2629 bits (199), Expect = 1.271e-16
Identity = 42/110 (38.18%), Postives = 58/110 (52.73%), Query Frame = 1 
Query:  382 KNNFKFRKIF---DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVW 702
            K + +F  IF       +E L+   SCAL  +IL QG +Y S     F+S I      V I  + I++I K  TAGIFPN I I T H ++ F+SF  R+  +  + EVW
Sbjct:  545 KKDAEFHAIFKDSGVSPNERLILDHSCALSRDILLQGRMYISDQHIGFYSNILGWVSTVFIPFKTIVQIEKRATAGIFPNGIVIDTLHTKYTFASFTSRDATYDLITEVW 654
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Yeast
Match: LAM6 (Sterol transporter that transfers sterols between membranes; may regulate and coordinate formation of contact sites between organelles; localizes to ER-mitochondrial contact sites in a Tom70p- and Tom71p-dependent manner; mitochondrial localization requires GRAM domain; also localizes to ER-vacuole contact sites, in a Vac8p-dependent manner; has GRAM and StART-like (VASt) domains; one of six StART-like domain-containing proteins in yeast; conserved across eukaryotes [Source:SGD;Acc:S000004062])

HSP 1 Score: 56.6102 bits (135), Expect = 6.369e-9
Identity = 33/107 (30.84%), Postives = 54/107 (50.47%), Query Frame = 1 
Query:  388 NFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWT 705
            N KFR++F     +  L+  + C    E  +QG +Y S    CF S + N   K+ I + +I  + K  T     +AI++ T  NR+ FS F+ R+  F+ +  VW+
Sbjct:  163 NKKFRQMFKPLAPNTRLITDYFCYFHREFPYQGRIYLSNTHLCFNSTVLNWMAKLQIPLNEIKYLDKVTTNS---SAISVETVTNRYTFSGFIARDEVFQLITRVWS 266
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Nematostella
Match: EDO45283 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RTK5])

HSP 1 Score: 50.0618 bits (118), Expect = 2.711e-8
Identity = 26/53 (49.06%), Postives = 36/53 (67.92%), Query Frame = 1 
Query:  373 YKSKNNFKFRKIF-DFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRI 528
            YKSK+   FR++F D  +SE L+  +SCAL  +IL  G LY SQN+ CF++ I
Sbjct:    5 YKSKSG-DFRRLFKDLPDSEQLIVDYSCALQRDILVHGRLYISQNWLCFYANI 56
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Nematostella
Match: EDO26770 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TB47])

HSP 1 Score: 52.7582 bits (125), Expect = 9.591e-8
Identity = 24/55 (43.64%), Postives = 35/55 (63.64%), Query Frame = 1 
Query:  544 KVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            +V I   +++ I KEKTA + PNAI I TE  ++ F+SF+ R+ AF  L  +W N
Sbjct:   18 QVTINCSEVISIRKEKTALVIPNAIQISTEGEKYFFASFISRDTAFTVLFRIWQN 72
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Medaka
Match: gramd1a (GRAM domain containing 1A [Source:NCBI gene;Acc:101170872])

HSP 1 Score: 93.9745 bits (232), Expect = 9.005e-20
Identity = 48/116 (41.38%), Postives = 71/116 (61.21%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRKIF    ++E L+  +SCAL  +IL QG LY S+++ CF+S I   +  + I ++ +  +TKEKTA + PNAI I TE  +H F+SF  R+ +F  +  +W N
Sbjct:  216 SPTYKQRNE-DFRKIFKKLPDTERLIVDYSCALQKDILLQGRLYLSESWLCFYSNIFRWETTITIQLKDVTCMTKEKTAKLIPNAIQISTESEKHFFTSFGARDRSFMMIFRLWQN 330
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Medaka
Match: gramd1a (GRAM domain containing 1A [Source:NCBI gene;Acc:101170872])

HSP 1 Score: 93.9745 bits (232), Expect = 9.005e-20
Identity = 48/116 (41.38%), Postives = 71/116 (61.21%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRKIF    ++E L+  +SCAL  +IL QG LY S+++ CF+S I   +  + I ++ +  +TKEKTA + PNAI I TE  +H F+SF  R+ +F  +  +W N
Sbjct:  216 SPTYKQRNE-DFRKIFKKLPDTERLIVDYSCALQKDILLQGRLYLSESWLCFYSNIFRWETTITIQLKDVTCMTKEKTAKLIPNAIQISTESEKHFFTSFGARDRSFMMIFRLWQN 330
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Medaka
Match: gramd1a (GRAM domain containing 1A [Source:NCBI gene;Acc:101170872])

HSP 1 Score: 93.9745 bits (232), Expect = 9.110e-20
Identity = 48/116 (41.38%), Postives = 71/116 (61.21%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRKIF    ++E L+  +SCAL  +IL QG LY S+++ CF+S I   +  + I ++ +  +TKEKTA + PNAI I TE  +H F+SF  R+ +F  +  +W N
Sbjct:  216 SPTYKQRNE-DFRKIFKKLPDTERLIVDYSCALQKDILLQGRLYLSESWLCFYSNIFRWETTITIQLKDVTCMTKEKTAKLIPNAIQISTESEKHFFTSFGARDRSFMMIFRLWQN 330
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Medaka
Match: gramd1a (GRAM domain containing 1A [Source:NCBI gene;Acc:101170872])

HSP 1 Score: 93.2041 bits (230), Expect = 1.424e-19
Identity = 48/116 (41.38%), Postives = 71/116 (61.21%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRKIF    ++E L+  +SCAL  +IL QG LY S+++ CF+S I   +  + I ++ +  +TKEKTA + PNAI I TE  +H F+SF  R+ +F  +  +W N
Sbjct:   84 SPTYKQRNE-DFRKIFKKLPDTERLIVDYSCALQKDILLQGRLYLSESWLCFYSNIFRWETTITIQLKDVTCMTKEKTAKLIPNAIQISTESEKHFFTSFGARDRSFMMIFRLWQN 198
[Close]
BLAST of GRAM domain-containing protein vs. Ensembl Medaka
Match: gramd1a (GRAM domain containing 1A [Source:NCBI gene;Acc:101170872])

HSP 1 Score: 92.8189 bits (229), Expect = 1.639e-19
Identity = 48/116 (41.38%), Postives = 71/116 (61.21%), Query Frame = 1 
Query:  364 SNLYKSKNNFKFRKIFD-FEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTN 708
            S  YK +N   FRKIF    ++E L+  +SCAL  +IL QG LY S+++ CF+S I   +  + I ++ +  +TKEKTA + PNAI I TE  +H F+SF  R+ +F  +  +W N
Sbjct:  120 SPTYKQRNE-DFRKIFKKLPDTERLIVDYSCALQKDILLQGRLYLSESWLCFYSNIFRWETTITIQLKDVTCMTKEKTAKLIPNAIQISTESEKHFFTSFGARDRSFMMIFRLWQN 234
[Close]
BLAST of GRAM domain-containing protein vs. Planmine SMEST
Match: SMESG000004908.1 (SMESG000004908.1)

HSP 1 Score: 711.064 bits (1834), Expect = 0.000e+0
Identity = 392/394 (99.49%), Postives = 392/394 (99.49%), Query Frame = 1 
Query:  232 MTQSINSESPTVSGSNIILSSATISRPAFSWCSKCGQSMEISTASNLYKSKNNFKFRKIFDFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTNREDERFPPQWNLGFGEETKDNSLIDIDVDTETLIIQAPVMSNILSSXXXXXXXXXXXXXXXXXXXRTRRTSKTFATFSPKLKDINVARDHRETGNSQTNLNCFIKNVFNSISKEKXXXXXXXXXXXXXXXXXXXXAWKIHDFQMILIDRFSTSCNHNKQQTFKNIECPTSDLSCKIHKDSIMEHQAKMSKVLSNLKDTLNLLQNVVEISGQMFSKLRPVSLSSIIGTNKNKPGDS 1413
            MTQSINSESPTVSGSNIILSSATISRPAFSWCSKCGQSMEISTASNLYKSKNNFKFRKIFDFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTNREDERFPPQWNLGFGEETKDNSLIDIDVDTETLIIQAPVMSNILS DDMV EDDEEDQIEEMEVIRTRRTSKTFATFSPKLKDINVARDHRETGNSQTNLNCFIKNVFNSISKEKIQLITAILILIFLTLYIVILAWKIHDFQMILIDRFSTSCNHNKQQTFKNIECPTSDLSCKIHKDSIMEHQAKMSKVLSNLKDTLNLLQNVVEISGQMFSKLRPVSLSSIIGTNKNKPGDS
Sbjct:    1 MTQSINSESPTVSGSNIILSSATISRPAFSWCSKCGQSMEISTASNLYKSKNNFKFRKIFDFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTNREDERFPPQWNLGFGEETKDNSLIDIDVDTETLIIQAPVMSNILSRDDMVEEDDEEDQIEEMEVIRTRRTSKTFATFSPKLKDINVARDHRETGNSQTNLNCFIKNVFNSISKEKIQLITAILILIFLTLYIVILAWKIHDFQMILIDRFSTSCNHNKQQTFKNIECPTSDLSCKIHKDSIMEHQAKMSKVLSNLKDTLNLLQNVVEISGQMFSKLRPVSLSSIIGTNKNKPGDS 394
[Close]
BLAST of GRAM domain-containing protein vs. Planmine SMEST
Match: SMESG000004908.1 (SMESG000004908.1)

HSP 1 Score: 638.262 bits (1645), Expect = 0.000e+0
Identity = 363/394 (92.13%), Postives = 363/394 (92.13%), Query Frame = 1 
Query:  232 MTQSINSESPTVSGSNIILSSATISRPAFSWCSKCGQSMEISTASNLYKSKNNFKFRKIFDFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTNREDERFPPQWNLGFGEETKDNSLIDIDVDTETLIIQAPVMSNILSSXXXXXXXXXXXXXXXXXXXRTRRTSKTFATFSPKLKDINVARDHRETGNSQTNLNCFIKNVFNSISKEKXXXXXXXXXXXXXXXXXXXXAWKIHDFQMILIDRFSTSCNHNKQQTFKNIECPTSDLSCKIHKDSIMEHQAKMSKVLSNLKDTLNLLQNVVEISGQMFSKLRPVSLSSIIGTNKNKPGDS 1413
            MTQSINSESPTVSGSNIILSSATISRPAFSWCSKCGQSMEISTASNLYKSKNNFKFRKIFDFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTNREDERFPPQW                             MSNILS DDMV EDDEEDQIEEMEVIRTRRTSKTFATFSPKLKDINVARDHRETGNSQTNLNCFIKNVFNSISKEKIQLITAILILIFLTLYIVILAWKIHDFQMILIDRFSTSCNHNKQQTFKNIECPTSDLSCKIHKDSIMEHQAKMSKVLSNLKDTLNLLQNVVEISGQMFSKLRPVSLSSIIGTNKNKPGDS
Sbjct:    1 MTQSINSESPTVSGSNIILSSATISRPAFSWCSKCGQSMEISTASNLYKSKNNFKFRKIFDFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAFRCLNEVWTNREDERFPPQW-----------------------------MSNILSRDDMVEEDDEEDQIEEMEVIRTRRTSKTFATFSPKLKDINVARDHRETGNSQTNLNCFIKNVFNSISKEKIQLITAILILIFLTLYIVILAWKIHDFQMILIDRFSTSCNHNKQQTFKNIECPTSDLSCKIHKDSIMEHQAKMSKVLSNLKDTLNLLQNVVEISGQMFSKLRPVSLSSIIGTNKNKPGDS 365
[Close]
BLAST of GRAM domain-containing protein vs. Planmine SMEST
Match: SMESG000033356.1 (SMESG000033356.1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.206e-16
Identity = 41/103 (39.81%), Postives = 66/103 (64.08%), Query Frame = 1 
Query:  421 ESEVLVKTFSCAL---VDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHN-RHLFSSFVHREIAFRCLNEVWTNRED 717
            ES  L+  +SCA+     +IL QG +Y +  F CF+SR    ++K+ ++ + I E+TKEKTA +F NAI I T++N +H FSSF  R+ A+  ++++W   +D
Sbjct:   22 ESSPLISDYSCAIYAGTPKILQQGRMYITTEFLCFYSRFITFEQKITVSYKDIKELTKEKTAMVFQNAIQIVTDNNEKHFFSSFTTRDRAYYVIHKLWMENKD 124
[Close]
BLAST of GRAM domain-containing protein vs. Planmine SMEST
Match: SMESG000033356.1 (SMESG000033356.1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.210e-16
Identity = 41/103 (39.81%), Postives = 66/103 (64.08%), Query Frame = 1 
Query:  421 ESEVLVKTFSCAL---VDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHN-RHLFSSFVHREIAFRCLNEVWTNRED 717
            ES  L+  +SCA+     +IL QG +Y +  F CF+SR    ++K+ ++ + I E+TKEKTA +F NAI I T++N +H FSSF  R+ A+  ++++W   +D
Sbjct:   28 ESSPLISDYSCAIYAGTPKILQQGRMYITTEFLCFYSRFITFEQKITVSYKDIKELTKEKTAMVFQNAIQIVTDNNEKHFFSSFTTRDRAYYVIHKLWMENKD 130
[Close]
BLAST of GRAM domain-containing protein vs. Planmine SMEST
Match: SMESG000033356.1 (SMESG000033356.1)

HSP 1 Score: 82.0333 bits (201), Expect = 2.470e-16
Identity = 41/103 (39.81%), Postives = 66/103 (64.08%), Query Frame = 1 
Query:  421 ESEVLVKTFSCAL---VDEILFQGTLYASQNFFCFFSRIANKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHN-RHLFSSFVHREIAFRCLNEVWTNRED 717
            ES  L+  +SCA+     +IL QG +Y +  F CF+SR    ++K+ ++ + I E+TKEKTA +F NAI I T++N +H FSSF  R+ A+  ++++W   +D
Sbjct:   22 ESSPLISDYSCAIYAGTPKILQQGRMYITTEFLCFYSRFITFEQKITVSYKDIKELTKEKTAMVFQNAIQIVTDNNEKHFFSSFTTRDRAYYVIHKLWMENKD 124
The following BLAST results are available for this feature:
BLAST of GRAM domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GRAMD1A6.307e-2141.27GRAM domain containing 1A [Source:HGNC Symbol;Acc:... [more]
GRAMD1B2.925e-2038.79GRAM domain containing 1B [Source:HGNC Symbol;Acc:... [more]
GRAMD1A3.346e-1942.02GRAM domain containing 1A [Source:HGNC Symbol;Acc:... [more]
GRAMD1A4.458e-1942.02GRAM domain containing 1A [Source:HGNC Symbol;Acc:... [more]
GRAMD1A6.441e-1942.24GRAM domain containing 1A [Source:HGNC Symbol;Acc:... [more]
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BLAST of GRAM domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ZC328.32.226e-1231.16pep chromosome:WBcel235:I:6409050:6417010:-1 gene:... [more]
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BLAST of GRAM domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GramD1B8.284e-1938.51gene:FBgn0085423 transcript:FBtr0332409[more]
GramD1B8.744e-1938.51gene:FBgn0085423 transcript:FBtr0112641[more]
GramD1B8.827e-1938.51gene:FBgn0085423 transcript:FBtr0112638[more]
GramD1B8.920e-1938.51gene:FBgn0085423 transcript:FBtr0112639[more]
GramD1B9.835e-1938.51gene:FBgn0085423 transcript:FBtr0306333[more]
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BLAST of GRAM domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gramd1a1.986e-2140.52GRAM domain containing 1A [Source:ZFIN;Acc:ZDB-GEN... [more]
gramd1a2.555e-2040.52GRAM domain containing 1A [Source:ZFIN;Acc:ZDB-GEN... [more]
AL953903.43.897e-1936.00GRAM domain containing 1Ba [Source:NCBI gene;Acc:5... [more]
gramd1bb2.351e-1838.79GRAM domain containing 1Bb [Source:ZFIN;Acc:ZDB-GE... [more]
gramd1bb2.628e-1838.79GRAM domain containing 1Bb [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of GRAM domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GRAMD1B1.306e-1840.52GRAM domain containing 1B [Source:NCBI gene;Acc:10... [more]
GRAMD1B1.433e-1840.52GRAM domain containing 1B [Source:NCBI gene;Acc:10... [more]
GRAMD1B1.468e-1840.52GRAM domain containing 1B [Source:NCBI gene;Acc:10... [more]
GRAMD1B1.519e-1840.52GRAM domain containing 1B [Source:NCBI gene;Acc:10... [more]
GRAMD1B1.533e-1840.52GRAM domain containing 1B [Source:NCBI gene;Acc:10... [more]
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BLAST of GRAM domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Gramd1a2.026e-2139.35GRAM domain containing 1A [Source:MGI Symbol;Acc:M... [more]
Gramd1a1.440e-1942.48GRAM domain containing 1A [Source:MGI Symbol;Acc:M... [more]
Gramd1a2.557e-1942.48GRAM domain containing 1A [Source:MGI Symbol;Acc:M... [more]
Gramd1a2.769e-1937.27GRAM domain containing 1A [Source:MGI Symbol;Acc:M... [more]
Gramd1b2.216e-1838.79GRAM domain containing 1B [Source:MGI Symbol;Acc:M... [more]
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BLAST of GRAM domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q3KR56|ASTRA_RAT1.922e-1837.27Protein Aster-A OS=Rattus norvegicus OX=10116 GN=G... [more]
sp|Q8VEF1|ASTRA_MOUSE1.937e-1837.27Protein Aster-A OS=Mus musculus OX=10090 GN=Gramd1... [more]
sp|Q96CP6|ASTRA_HUMAN2.141e-1842.02Protein Aster-A OS=Homo sapiens OX=9606 GN=GRAMD1A... [more]
sp|Q80TI0|ASTRB_MOUSE1.922e-1738.79Protein Aster-B OS=Mus musculus OX=10090 GN=Gramd1... [more]
sp|Q3KR37|ASTRB_HUMAN1.922e-1734.64Protein Aster-B OS=Homo sapiens OX=9606 GN=GRAMD1B... [more]
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BLAST of GRAM domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8G5751.380e-2754.37GRAM domain-containing protein OS=Macrostomum lign... [more]
A0A1I8J0U71.548e-2754.37GRAM domain-containing protein OS=Macrostomum lign... [more]
A0A267G4X23.008e-2754.37GRAM domain-containing protein OS=Macrostomum lign... [more]
A0A267GQY93.142e-2754.37GRAM domain-containing protein OS=Macrostomum lign... [more]
A0A1Q3G1V52.674e-2042.73Protein in glucosyltransferase OS=Culex tarsalis O... [more]
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BLAST of GRAM domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000038267.14.683e-2140.52pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000012808.25.374e-1936.84GRAM domain-containing protein 2B-like [Source:NCB... [more]
ENSAMXT00000057118.16.624e-1936.84GRAM domain-containing protein 2B-like [Source:NCB... [more]
ENSAMXT00000042002.16.884e-1936.84GRAM domain-containing protein 2B-like [Source:NCB... [more]
gramd2aa9.666e-1934.62GRAM domain-containing protein 2A [Source:NCBI gen... [more]
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BLAST of GRAM domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gramd1bb8.796e-2143.10GRAM domain containing 1Bb [Source:ZFIN;Acc:ZDB-GE... [more]
GRAMD1A5.833e-1838.79GRAM domain containing 1A [Source:HGNC Symbol;Acc:... [more]
ENSPMAT00000003678.16.014e-1637.36pep scaffold:Pmarinus_7.0:GL477104:103280:105081:-... [more]
TBC1D91.582e-835.16TBC1 domain family member 9 [Source:HGNC Symbol;Ac... [more]
ENSPMAT00000006389.18.146e-829.13pep scaffold:Pmarinus_7.0:GL481951:143257:161898:-... [more]
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BLAST of GRAM domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4
Match NameE-valueIdentityDescription
YSP23.195e-1840.00Sterol-binding protein; has a probable role in ret... [more]
YFL042C_mRNA9.906e-1739.56Putative sterol transfer protein; one of six StART... [more]
LAM41.271e-1638.18Sterol-binding protein that localizes to puncta in... [more]
LAM66.369e-930.84Sterol transporter that transfers sterols between ... [more]
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BLAST of GRAM domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO452832.711e-849.06Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO267709.591e-843.64Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of GRAM domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gramd1a9.005e-2041.38GRAM domain containing 1A [Source:NCBI gene;Acc:10... [more]
gramd1a9.005e-2041.38GRAM domain containing 1A [Source:NCBI gene;Acc:10... [more]
gramd1a9.110e-2041.38GRAM domain containing 1A [Source:NCBI gene;Acc:10... [more]
gramd1a1.424e-1941.38GRAM domain containing 1A [Source:NCBI gene;Acc:10... [more]
gramd1a1.639e-1941.38GRAM domain containing 1A [Source:NCBI gene;Acc:10... [more]
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BLAST of GRAM domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000004908.10.000e+099.49SMESG000004908.1[more]
SMESG000004908.10.000e+092.13SMESG000004908.1[more]
SMESG000033356.12.206e-1639.81SMESG000033356.1[more]
SMESG000033356.12.210e-1639.81SMESG000033356.1[more]
SMESG000033356.12.470e-1639.81SMESG000033356.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002002 ID=SMED30002002|Name=GRAM domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1534bp
ATTACACACTTCGTGGCAGCTACATTTCTGAAAATAAGAGTTTCAGTCGA
AAACTGAATAAATCACATTCAACTGATCTATCGATTTAGAATCAGCTGTG
TTGATTTGATGCTAATATTAGAAAACGCATTAATTTAATGTTAATTTAAG
TGTTTAATTGTGGTTTACGGATATATCCACTTGAGTTTAAATGCGTGTTG
ATAATCTAAAATTTAATCGTATGTCATATCCATGACTCAATCAATCAACT
CAGAGTCACCAACTGTTTCTGGATCTAACATCATTTTATCCAGTGCCACC
ATATCTCGCCCTGCATTTAGTTGGTGTTCCAAATGTGGCCAGAGCATGGA
AATTAGTACGGCTTCCAATCTATATAAAAGTAAAAACAACTTCAAATTTA
GAAAGATATTCGATTTTGAAGAATCTGAAGTTTTGGTGAAAACTTTCTCA
TGTGCATTAGTCGACGAAATACTATTTCAAGGCACATTATACGCATCACA
AAATTTCTTCTGCTTTTTCTCAAGAATAGCAAATAAGCAAAAGAAGGTTA
TTATAGCGATTGAAAAAATATTAGAAATAACAAAAGAGAAAACCGCTGGA
ATTTTCCCAAATGCCATTGCAATAAGAACAGAACACAATCGACATTTGTT
TTCATCGTTTGTGCATCGGGAAATCGCATTCAGGTGTCTCAATGAGGTTT
GGACTAATCGTGAAGATGAGAGGTTTCCTCCACAGTGGAATTTGGGCTTC
GGTGAAGAAACGAAAGATAATTCCTTAATAGATATTGACGTGGACACTGA
AACTTTGATTATTCAGGCGCCAGTGATGTCAAACATATTATCCAGTGATG
ATATGGTAGTGGAAGATGATGAAGAAGATCAAATTGAAGAAATGGAAGTA
ATCAGAACTAGAAGAACTTCAAAAACTTTTGCTACATTTTCGCCCAAGCT
AAAAGACATAAATGTTGCTCGAGATCACCGCGAAACCGGCAACTCTCAAA
CAAATCTAAACTGCTTTATTAAAAATGTATTTAATTCCATATCCAAAGAA
AAGATTCAACTTATTACTGCGATTTTAATATTAATATTTTTGACACTCTA
CATAGTCATTTTGGCGTGGAAAATCCACGACTTCCAGATGATTCTGATTG
ACAGATTCTCCACATCATGCAACCACAATAAACAACAAACTTTCAAAAAC
ATTGAATGCCCGACTTCTGATTTGTCATGCAAAATTCACAAGGACTCAAT
CATGGAGCATCAAGCAAAAATGTCGAAAGTCTTATCCAATCTGAAAGATA
CATTAAATTTATTGCAAAATGTTGTTGAAATTTCAGGACAAATGTTTAGT
AAACTACGTCCGGTATCGTTATCTAGTATTATAGGAACAAATAAAAATAA
ACCAGGGGACAGTTGACGTATAATAAGTCATATTAAAATGTAAATTAATT
TCGCAACGCAAAGTATTGGCTTTATTTAATTTTTCTTTTCAATTAGGTTT
TCAGTAATAATTTTTACTATTGTTATAATCTATC
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protein sequence of SMED30002002-orf-1

>SMED30002002-orf-1 ID=SMED30002002-orf-1|Name=SMED30002002-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=395bp
MTQSINSESPTVSGSNIILSSATISRPAFSWCSKCGQSMEISTASNLYKS
KNNFKFRKIFDFEESEVLVKTFSCALVDEILFQGTLYASQNFFCFFSRIA
NKQKKVIIAIEKILEITKEKTAGIFPNAIAIRTEHNRHLFSSFVHREIAF
RCLNEVWTNREDERFPPQWNLGFGEETKDNSLIDIDVDTETLIIQAPVMS
NILSSDDMVVEDDEEDQIEEMEVIRTRRTSKTFATFSPKLKDINVARDHR
ETGNSQTNLNCFIKNVFNSISKEKIQLITAILILIFLTLYIVILAWKIHD
FQMILIDRFSTSCNHNKQQTFKNIECPTSDLSCKIHKDSIMEHQAKMSKV
LSNLKDTLNLLQNVVEISGQMFSKLRPVSLSSIIGTNKNKPGDS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR011993PH-like_dom_sf
IPR004182GRAM
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004182GRAM domainSMARTSM00568gram2001ccoord: 54..120
e-value: 1.1E-14
score: 64.8
IPR004182GRAM domainPFAMPF02893GRAMcoord: 55..160
e-value: 2.4E-18
score: 66.1
IPR011993PH-like domain superfamilyGENE3DG3DSA:2.30.29.30coord: 51..160
e-value: 4.7E-30
score: 105.7
NoneNo IPR availablePANTHERPTHR23319UNCHARACTERIZEDcoord: 21..162
NoneNo IPR availableCDDcd13220PH-GRAM_GRAMDCcoord: 64..157
e-value: 1.85727E-35
score: 123.385
NoneNo IPR availableTMHMMTMhelixcoord: 277..296