POU domain protein

Overview
NamePOU domain protein
Smed IDSMED30001958
Length (bp)1820
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30001958

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30001958SMESG000057970.1 dd_Smed_v4_22370_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30001958SMESG000057970.1 dd_Smed_v4_22370_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30001958SMESG000057970.1 dd_Smed_v4_22370_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30001958SMESG000057970.1 dd_Smed_v6_22370_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of POU domain protein vs. UniProt/SwissProt
Match: sp|P31370|POU1_DUGJA (POU domain protein 1 OS=Dugesia japonica OX=6161 GN=POU1 PE=2 SV=1)

HSP 1 Score: 425.631 bits (1093), Expect = 1.371e-141
Identity = 201/243 (82.72%), Postives = 226/243 (93.00%), Query Frame = 2 
Query:  221 MTQVSHIGSSHFNINSSIKTALPLINSQYNSLVTETSIGSNINLPFRLDAPSLFNGLPEEWGPTDSELTQAVNHVNYAGLSESSIDYKFCSTLNSYFDSNQRFGNVPNDTYLMANSMAANIENNSWHNSRYYFDSNHPLSQWLPVPVNCWSNNNYIRDDFYEKSLQEDEFKSRSVIQATEKNSNPVNPDLNPYKSIISDESESMKMAKTSHNLINNDSMNMCRFPQNDIIMPDLINAPSPSLT 949
            MTQVSHIGSSHFNINSSIKTALPLINSQYNSLV E++I SNINLPFRLDAPSLFNGLPEEWGPTD+EL+   N VNY GL+E++IDYKFCSTLNSYFD+NQRFGNVPNDTYLMANSMAANIENNSWHNSRYYFD NHPLSQWLPVPVNCWSNNNYIRDDFY+KS+QE+EFKSRSV+Q  EKNS  V+ D+N YK +I+DE+E++KM K +HNL+NNDS+N+CRFPQN+IIMPDLINAPSP+LT
Sbjct:    1 MTQVSHIGSSHFNINSSIKTALPLINSQYNSLVNESTISSNINLPFRLDAPSLFNGLPEEWGPTDAELSTHSN-VNYPGLTEATIDYKFCSTLNSYFDTNQRFGNVPNDTYLMANSMAANIENNSWHNSRYYFDGNHPLSQWLPVPVNCWSNNNYIRDDFYDKSIQEEEFKSRSVLQTNEKNSTNVSSDINHYKPLITDENENLKMTKPTHNLLNNDSINLCRFPQNEIIMPDLINAPSPTLT 242
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BLAST of POU domain protein vs. TrEMBL
Match: Q94551 (POU domain protein OS=Girardia tigrina OX=6162 GN=GtPOU-1 PE=3 SV=2)

HSP 1 Score: 408.297 bits (1048), Expect = 4.142e-133
Identity = 193/244 (79.10%), Postives = 223/244 (91.39%), Query Frame = 2 
Query:  221 MTQVSHIGSSHFNINSSIKTALPLINSQYNSLVTETSIGSNINLPFRLDAPSLFNGLPEEWGPTDSELT-QAVNHVNYAGLSESSIDYKFCSTLNSYFDSNQRFGNVPNDTYLMANSMAANIENNSWHNSRYYFDSNHPLSQWLPVPVNCWSNNNYIRDDFYEKSLQEDEFKSRSVIQATEKNSNPVNPDLNPYKSIISDESESMKMAKTSHNLINNDSMNMCRFPQNDIIMPDLINAPSPSLT 949
            MTQV+HIG SHFNINSSIKTALPLINSQY+SLV E+SIGSNINLPFRLDAP LFNGLPEEWGP D E++  + NH+NYAGL+ESSIDYKFCSTLNSYFDSNQRFGN+ NDTYLMANSMA+NIENN WHN RYYFD  HPLSQWLPVPVNCW+NNNYIR+DFYEKSLQEDEFK+RSVI + +KNS  ++ D+N YK +I DE++++KMAK+SH+L+NNDSM+MCRFPQN+IIMPDL+NAPSP+LT
Sbjct:    1 MTQVTHIGPSHFNINSSIKTALPLINSQYSSLVNESSIGSNINLPFRLDAP-LFNGLPEEWGPPDPEISNHSNNHMNYAGLTESSIDYKFCSTLNSYFDSNQRFGNMTNDTYLMANSMASNIENNGWHNGRYYFDGTHPLSQWLPVPVNCWTNNNYIREDFYEKSLQEDEFKTRSVIPSNDKNSANISSDINHYKPLIPDEADALKMAKSSHSLLNNDSMSMCRFPQNEIIMPDLMNAPSPTLT 243
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BLAST of POU domain protein vs. Planmine SMEST
Match: SMESG000057970.1 (SMESG000057970.1)

HSP 1 Score: 502.671 bits (1293), Expect = 1.057e-172
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 2 
Query:  221 MTQVSHIGSSHFNINSSIKTALPLINSQYNSLVTETSIGSNINLPFRLDAPSLFNGLPEEWGPTDSELTQAVNHVNYAGLSESSIDYKFCSTLNSYFDSNQRFGNVPNDTYLMANSMAANIENNSWHNSRYYFDSNHPLSQWLPVPVNCWSNNNYIRDDFYEKSLQEDEFKSRSVIQATEKNSNPVNPDLNPYKSIISDESESMKMAKTSHNLINNDSMNMCRFPQNDIIMPDLINAPSPSLT 949
            MTQVSHIGSSHFNINSSIKTALPLINSQYNSLVTETSIGSNINLPFRLDAPSLFNGLPEEWGPTDSELTQAVNHVNYAGLSESSIDYKFCSTLNSYFDSNQRFGNVPNDTYLMANSMAANIENNSWHNSRYYFDSNHPLSQWLPVPVNCWSNNNYIRDDFYEKSLQEDEFKSRSVIQATEKNSNPVNPDLNPYKSIISDESESMKMAKTSHNLINNDSMNMCRFPQNDIIMPDLINAPSPSLT
Sbjct:    1 MTQVSHIGSSHFNINSSIKTALPLINSQYNSLVTETSIGSNINLPFRLDAPSLFNGLPEEWGPTDSELTQAVNHVNYAGLSESSIDYKFCSTLNSYFDSNQRFGNVPNDTYLMANSMAANIENNSWHNSRYYFDSNHPLSQWLPVPVNCWSNNNYIRDDFYEKSLQEDEFKSRSVIQATEKNSNPVNPDLNPYKSIISDESESMKMAKTSHNLINNDSMNMCRFPQNDIIMPDLINAPSPSLT 243
The following BLAST results are available for this feature:
BLAST of POU domain protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of POU domain protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of POU domain protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of POU domain protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of POU domain protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of POU domain protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of POU domain protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|P31370|POU1_DUGJA1.371e-14182.72POU domain protein 1 OS=Dugesia japonica OX=6161 G... [more]
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BLAST of POU domain protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
Q945514.142e-13379.10POU domain protein OS=Girardia tigrina OX=6162 GN=... [more]
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BLAST of POU domain protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of POU domain protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of POU domain protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of POU domain protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of POU domain protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of POU domain protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000057970.11.057e-172100.00SMESG000057970.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001958 ID=SMED30001958|Name=POU domain protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1820bp
GAAGATTTCAACCAATTTCATTGTTAAAATTTTCATAAAATCGCCGTAAT
TGGCTGTCCGTTGCGGCAGTGGCAGTCAGGCAACTCCGTTCACTCAGCAG
CCAATAAACTCGTTTGGAAATATTCCTACTCAAATCAAATTGATTTCTCA
TAAAAAAATTTGCATATCTTCTCACTTTTTCTATATGCATTTCATTGTTA
CTTGGTGAAAACTGAATGCCATGACCCAAGTTAGTCACATAGGTTCCTCT
CATTTCAATATTAATTCTTCAATCAAAACTGCTCTTCCTTTAATTAATTC
TCAATACAATTCATTGGTAACCGAAACATCTATTGGTTCCAACATCAACC
TACCGTTTCGACTCGATGCTCCATCTCTATTCAATGGGCTACCGGAAGAA
TGGGGTCCCACTGACAGCGAGTTAACTCAAGCGGTCAATCATGTAAATTA
CGCCGGATTATCCGAGTCGAGCATTGATTACAAGTTTTGCAGCACTCTCA
ATTCTTATTTTGACTCAAATCAAAGATTTGGAAATGTACCAAACGACACG
TATTTAATGGCAAATTCCATGGCAGCCAACATTGAGAACAATAGCTGGCA
TAACAGCCGATATTACTTCGACAGTAATCATCCTCTCAGCCAATGGCTCC
CGGTGCCCGTTAACTGTTGGTCCAATAACAATTACATTCGCGATGACTTT
TACGAGAAATCTCTCCAGGAAGATGAGTTCAAATCCCGCTCGGTAATACA
AGCAACTGAGAAGAATTCCAATCCGGTAAATCCCGACCTGAATCCTTACA
AATCTATCATTTCCGATGAATCAGAAAGCATGAAAATGGCTAAAACCTCC
CACAACCTTATAAATAATGACTCGATGAATATGTGTAGATTTCCTCAAAA
TGATATTATTATGCCAGATTTAATAAATGCCCCAAGTCCATCACTGACAA
ATAATAATAATAATAATAAAGTAATAAATAATTATTATATAATGAAAATT
AATATATTAATAAAAAATATTTATGCACGGTCTCAATTTACATCTAAATG
CCATCAAATTTGGCAGGGATACTTCTAATATAAAACCTAGATCAGCTGAA
AGACTTTTATATTAGATAAATAATCGTTAATAAATAAAACCCAATTTGCG
ATAAATTATGTGTGGCCTCCAGGCGGTTCCTATCAAATAAACATATGTAA
TTATACGTGTAAACACGTGCATTAAATATCTTCACAAAGTCAAATGAGTT
GATTGGACAGAAAAATTAGAATCGGAACTAATTCTGCAATCATTCAAAAA
TTTGAGCTAATCTTTATTCTTTAAAAAGCAGAGAATCAGCGGTTCTTTAC
ATTCATGCCAGTAATGAAAAAAGTAAACCGCAAATGACTTTTATTACTGT
TCTAGTAAATGCATCCCCATAAGGAGAGTAATTCACATAAAACTTTAATT
GTCAATAAAATTATTACTGGGACATCAGCGCATCCTGGCATGCACCGCAG
CAATGAAAAAGTAGACCGAGAAACTCTGAGATTATTGAGCCATCAAACGT
ATCACTCATTTTCTTATGTTTGAATAAAATATTTACAAATTTTAATACAG
AGATAATCGATGGAGGAGGAACACTACTATTTTTGCTATCTATAGCAATC
AATAATTATGTGAAAATATAGCTGATCAAAACAAGATGCATTAAAACAAT
TTTACTTCCTATTATATACGTTTTTGAATCCTGAGTGAAGCCAGTCGGGT
TAACGAATATTATTAAAATGAAAATAACTATAAATCAATAACTATACCTC
ATCACATGATGAATGGTCCA
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protein sequence of SMED30001958-orf-1

>SMED30001958-orf-1 ID=SMED30001958-orf-1|Name=SMED30001958-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=286bp
MTQVSHIGSSHFNINSSIKTALPLINSQYNSLVTETSIGSNINLPFRLDA
PSLFNGLPEEWGPTDSELTQAVNHVNYAGLSESSIDYKFCSTLNSYFDSN
QRFGNVPNDTYLMANSMAANIENNSWHNSRYYFDSNHPLSQWLPVPVNCW
SNNNYIRDDFYEKSLQEDEFKSRSVIQATEKNSNPVNPDLNPYKSIISDE
SESMKMAKTSHNLINNDSMNMCRFPQNDIIMPDLINAPSPSLTNNNNNNK
VINNYYIMKINILIKNIYARSQFTSKCHQIWQGYF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
GO:0003700transcription factor activity, sequence-specific DNA binding
GO:0043565sequence-specific DNA binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
Vocabulary: biological process
TermDefinition
GO:0006355regulation of transcription, DNA-templated