SMED30001879

Overview
NameSMED30001879
Smed IDSMED30001879
Length (bp)500
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30001879

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 22

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30001879h1SMcG0014521 SmedASXL_072889SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30001879h1SMcG0014521 SmedASXL_072889SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30001879h1SMcG0014521 SmedASXL_050040SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30001879h1SMcG0014521 SmedASXL_050040SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30001879h1SMcG0014523 SmedASXL_072889SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30001879h1SMcG0014523 SmedASXL_072889SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30001879h1SMcG0014521 SmedASXL_073611SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30001879h1SMcG0014521 SmedASXL_073611SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30001879h1SMcG0014521 SmedASXL_073612SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30001879h1SMcG0014521 SmedASXL_073612SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30001879h1SMcG0014521 SmedASXL_011934SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30001879h1SMcG0014521 SmedASXL_011934SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30001879h1SMcG0014521 SmedASXL_015040SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30001879h1SMcG0014521 SmedASXL_015040SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30001879h1SMcG0014521 SmedASXL_011934SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult colorimetric in situ hybridization evidence
Smed sexual biotypeSMED30001879h1SMcG0014521 Contig42421uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001879h1SMcG0014521 Contig42421newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001879h1SMcG0014521 Contig54424uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001879h1SMcG0014521 Contig54424newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
parapharyngeal regionSMED30001879h1SMcG0014521 dd_Smed_v6_12898_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30001879h1SMcG0014521 OX_Smed_1.0.11315ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30001879h1SMcG0014521 OX_Smed_1.0.09820ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30001879 vs. TrEMBL
Match: A0A1S6KMJ1 (Uncharacterized protein (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 5.945e-29
Identity = 86/128 (67.19%), Postives = 106/128 (82.81%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKF 386
            IKWNW DLIKNID K+RE  +KEI++VLKLVKENKFKEIDENLYK+IRVI+K IRK+  DK P ++I++QKE  L KLK II  NW K+INETK ID+VKW +RIN +I+L+KLN+ DKIDL WL++ 
Sbjct:   25 IKWNWPDLIKNIDVKERERFQKEIDIVLKLVKENKFKEIDENLYKKIRVIYKKIRKVIKDKLPKVEIQIQKENSLEKLKPIITQNWAKIINETKNIDRVKWIDRINTIIKLVKLNEVDKIDLTWLKEL 152
[Close]
BLAST of SMED30001879 vs. TrEMBL
Match: A0A1S6KMJ8 (Uncharacterized protein (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.408e-18
Identity = 83/165 (50.30%), Postives = 113/165 (68.48%), Query Frame = 3 
Query:    6 KWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            +WNW +L+ +++EK++EN+ KEIEL+ +++KE  F EI+ +L KR +VIFK +RKIK  K P  +IKV KE LL K+KLIID+NW  +IN  + +DKVKWT R NKLIQ+I+ N  DKID KWL KF      + K   +K    K     +K+F+ RL KWNWP
Sbjct:  443 RWNWKNLVVDLNEKEKENIAKEIELMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNIKVTKEILLKKIKLIIDSNWLNIINGLEEVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKF----KRILKLIHRKSHPRKQKLNLIKKFVNRLKKWNWP 603
[Close]
BLAST of SMED30001879 vs. Planmine SMEST
Match: SMESG000008835.1 (SMESG000008835.1)

HSP 1 Score: 204.142 bits (518), Expect = 4.195e-61
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            IKWNWSDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWP
Sbjct: 1078 IKWNWSDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWP 1243

HSP 2 Score: 204.142 bits (518), Expect = 4.195e-61
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            IKWNWSDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWP
Sbjct: 1558 IKWNWSDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWP 1723

HSP 3 Score: 202.216 bits (513), Expect = 2.113e-60
Identity = 165/166 (99.40%), Postives = 165/166 (99.40%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            IKWNW DLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWP
Sbjct:  598 IKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWP 763

HSP 4 Score: 200.29 bits (508), Expect = 8.519e-60
Identity = 163/166 (98.19%), Postives = 164/166 (98.80%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            IKWNW DLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDK+PIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKN IKKPITVKIITKNVKRFIKRLIKWNWP
Sbjct:  758 IKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNVIKKPITVKIITKNVKRFIKRLIKWNWP 923

HSP 5 Score: 200.29 bits (508), Expect = 8.519e-60
Identity = 163/166 (98.19%), Postives = 164/166 (98.80%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            IKWNW DLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDK+PIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKN IKKPITVKIITKNVKRFIKRLIKWNWP
Sbjct: 1238 IKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNVIKKPITVKIITKNVKRFIKRLIKWNWP 1403

HSP 6 Score: 194.512 bits (493), Expect = 1.068e-57
Identity = 150/166 (90.36%), Postives = 159/166 (95.78%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            IKWNW DLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDK+PIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKF R+I IV+K A K+PIT+K+ITK+VK+F+ RLIKWNW 
Sbjct:  918 IKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWS 1083

HSP 7 Score: 194.512 bits (493), Expect = 1.068e-57
Identity = 150/166 (90.36%), Postives = 159/166 (95.78%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            IKWNW DLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDK+PIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKF R+I IV+K A K+PIT+K+ITK+VK+F+ RLIKWNW 
Sbjct: 1398 IKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWS 1563

HSP 8 Score: 107.457 bits (267), Expect = 2.281e-27
Identity = 85/125 (68.00%), Postives = 98/125 (78.40%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWL 377
            IKWNW DLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFK +RK+K DK P    ++ KE++L KLK I++ NW K+I ++  IDK KWT RI  + +LI   Q DKID  WL
Sbjct: 1718 IKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKKVRKVKIDKIPTEKKQLLKEKILTKLKPILNENWTKIIEKSTNIDKTKWTTRIKTITELINSKQTDKIDYSWL 1842

HSP 9 Score: 96.2857 bits (238), Expect = 1.947e-23
Identity = 83/165 (50.30%), Postives = 115/165 (69.70%), Query Frame = 3 
Query:    6 KWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            +WNW +L+ +++EK++EN+ KEIEL+ +++KE  F EI+ +L KR +VIFK +RKIK  K P  ++KV KE LL K+KLIID+NW  +IN  +G+DKVKWT R NK+IQ+I+ N  DKID KWL KF      + K   +K    K     +K+F+ RLIKWNWP
Sbjct:  443 RWNWKNLVVDLNEKEKENIAKEIELMKRIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNVKVTKEILLKKIKLIIDSNWLNIINGLEGVDKVKWTTRFNKIIQMIRNNNIDKIDFKWLLKF----KRILKLIHRKSHPRKQKLNLIKKFVNRLIKWNWP 603

HSP 10 Score: 52.373 bits (124), Expect = 3.151e-8
Identity = 25/59 (42.37%), Postives = 43/59 (72.88%), Query Frame = 3 
Query:  210 QKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKF 386
            +K  ++ +LKLI+  +W+++INE +GID+ +W  R NKL  LI+L + ++ID+ WL +F
Sbjct:  351 KKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNEIDVNWLAEF 409

HSP 11 Score: 48.521 bits (114), Expect = 5.994e-7
Identity = 48/168 (28.57%), Postives = 88/168 (52.38%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKW---LQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNW 497
            I+  W +L+++ID   R  V  +I+  +  V+   F +ID NL   I  I   +RKI+  +  + + K  K  +   ++L + +NWQ+++    GID   WT+RI++L+ ++K     KID +W   L+K   +I+       K+    +   + +K F+ +L +WNW
Sbjct: 2307 IELKWEELVQHIDSSSRVEVINKIKTFISFVQNKSFDKIDVNLANWIAEIINILRKIRIGQSKVTENKTLK--ITENIQLTLPSNWQELLQRINGIDYQLWTSRIDQLLTIMKSKNHQKIDFEWIGELEKIVNLISQESIKMKKEVFLNETSIEKLKGFVSQLKEWNW 2472
[Close]
BLAST of SMED30001879 vs. Planmine SMEST
Match: SMESG000008843.1 (SMESG000008843.1)

HSP 1 Score: 202.986 bits (515), Expect = 1.073e-60
Identity = 165/166 (99.40%), Postives = 165/166 (99.40%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            IKWNW DLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWP
Sbjct:  525 IKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWP 690

HSP 2 Score: 199.904 bits (507), Expect = 1.409e-59
Identity = 152/166 (91.57%), Postives = 160/166 (96.39%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            IKWNW DLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKF R+I IV+K A K+PIT+K+ITK+VK+F+ RLIKWNWP
Sbjct:  845 IKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWP 1010

HSP 3 Score: 199.904 bits (507), Expect = 1.409e-59
Identity = 152/166 (91.57%), Postives = 160/166 (96.39%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            IKWNW DLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKF R+I IV+K A K+PIT+K+ITK+VK+F+ RLIKWNWP
Sbjct: 1165 IKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWP 1330

HSP 4 Score: 198.364 bits (503), Expect = 4.592e-59
Identity = 151/166 (90.96%), Postives = 160/166 (96.39%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            IKWNW DLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDK+PIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKF R+I IV+K A K+PIT+K+ITK+VK+F+ RLIKWNWP
Sbjct: 1005 IKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWP 1170

HSP 5 Score: 198.364 bits (503), Expect = 4.592e-59
Identity = 151/166 (90.96%), Postives = 160/166 (96.39%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            IKWNW DLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDK+PIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKF R+I IV+K A K+PIT+K+ITK+VK+F+ RLIKWNWP
Sbjct: 1466 IKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWP 1631

HSP 6 Score: 195.282 bits (495), Expect = 4.644e-58
Identity = 150/166 (90.36%), Postives = 158/166 (95.18%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            IKWNW DLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDK+PIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKF +IIN+++KN  +KPITVK ITK++KR   RLIKWNWP
Sbjct: 1786 IKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNKIINVIRKNTTEKPITVKFITKDIKRITNRLIKWNWP 1951

HSP 7 Score: 179.489 bits (454), Expect = 1.980e-52
Identity = 151/166 (90.96%), Postives = 160/166 (96.39%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            IKWNW DLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKE+LLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKF R+I IV+K A K+PIT+K+ITK+VK+F+ RLIKWNWP
Sbjct:  685 IKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKEKLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWP 850

HSP 8 Score: 179.489 bits (454), Expect = 1.980e-52
Identity = 151/166 (90.96%), Postives = 160/166 (96.39%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            IKWNW DLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKE+LLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKF R+I IV+K A K+PIT+K+ITK+VK+F+ RLIKWNWP
Sbjct: 1626 IKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKEKLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWP 1791

HSP 9 Score: 154.451 bits (389), Expect = 9.591e-44
Identity = 133/166 (80.12%), Postives = 141/166 (84.94%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFXXXXXXXXXXXXXXXXXXXXXXXXXXRFIKRLIKWNWP 500
            IKWNW DLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIK                   KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKF R+I IV+K A K+PIT+K+ITK+VK+F+ RLIKWNWP
Sbjct: 1325 IKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIK-------------------KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWP 1471

HSP 10 Score: 120.939 bits (302), Expect = 4.309e-32
Identity = 86/128 (67.19%), Postives = 106/128 (82.81%), Query Frame = 3 
Query:    3 IKWNWSDLIKNIDEKDRXXXXXXXXXXXXXXXXXXXXXIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKF 386
            IKWNW DLIKNID K+RE  +KEI++VLKLVKENKFKEIDENLYK+IRVI+K IRK+  DK P ++I++QKE  L KLK II  NW K+INETK ID+VKW +RIN +I+L+KLN+ DKIDL WL++ 
Sbjct: 1946 IKWNWPDLIKNIDVKERERFQKEIDIVLKLVKENKFKEIDENLYKKIRVIYKKIRKVIKDKLPKVEIQIQKENSLEKLKPIITKNWAKIINETKNIDRVKWIDRINTIIKLVKLNEVDKIDLTWLKEL 2073
The following BLAST results are available for this feature:
BLAST of SMED30001879 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30001879 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30001879 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30001879 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30001879 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30001879 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30001879 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30001879 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 2
Match NameE-valueIdentityDescription
A0A1S6KMJ15.945e-2967.19Uncharacterized protein (Fragment) OS=Schmidtea me... [more]
A0A1S6KMJ81.408e-1850.30Uncharacterized protein (Fragment) OS=Schmidtea me... [more]
back to top
BLAST of SMED30001879 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30001879 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30001879 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30001879 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30001879 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30001879 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000008835.14.195e-61100.00SMESG000008835.1[more]
SMESG000008843.11.073e-6099.40SMESG000008843.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001879 ID=SMED30001879|Name=SMED30001879|organism=Schmidtea mediterranea sexual|type=transcript|length=500bp
TGATAAAATGGAACTGGTCGGATCTTATCAAAAATATCGATGAAAAAGAT
AGAGAAAACGTTAAAAAAGAAATCGAATTAGTATTGAAACTAGTGAAAGA
AAACAAATTCAAAGAAATAGATGAAAATCTTTACAAACGAATCCGAGTCA
TCTTCAAAACTATTCGAAAGATCAAAAATGATAAAAGACCCATCATAGAT
ATAAAAGTTCAAAAAGAAAGATTGTTGAACAAACTTAAGCTCATCATCGA
TACAAACTGGCAGAAAGTCATCAATGAGACAAAAGGAATAGATAAAGTCA
AATGGACAAATCGTATCAATAAACTCATTCAATTGATCAAACTTAATCAA
CCGGATAAAATTGATTTGAAATGGCTTCAGAAATTCAAAAGAATAATCAA
CATTGTCAAAAAGAATGCTATTAAAAAACCAATAACAGTAAAGATAATTA
CAAAAAATGTCAAAAGGTTTATTAAAAGATTGATAAAATGGAACTGGCCA
back to top

protein sequence of SMED30001879-orf-1

>SMED30001879-orf-1 ID=SMED30001879-orf-1|Name=SMED30001879-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=166bp
IKWNWSDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVI
FKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVK
WTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNAIKKPITVKIIT
KNVKRFIKRLIKWNWP
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000420parapharyngeal region