Dynein light chain roadblock

Overview
NameDynein light chain roadblock
Smed IDSMED30001875
Length (bp)532
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Dynein light chain roadblock (SMED30001875) t-SNE clustered cells

Violin plots show distribution of expression levels for Dynein light chain roadblock (SMED30001875) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Dynein light chain roadblock (SMED30001875) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Dynein light chain roadblock (SMED30001875) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30001875

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30001875SMESG000072361.1 dd_Smed_v4_1126_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30001875SMESG000072361.1 dd_Smed_v4_1126_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30001875SMESG000072361.1 dd_Smed_v4_1126_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30001875SMESG000072361.1 dd_Smed_v4_1126_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30001875SMESG000072361.1 dd_Smed_v4_1126_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
Smed sexual biotypeSMED30001875SMESG000072361.1 Contig42988newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001875SMESG000072361.1 Contig42988uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30001875SMESG000072361.1 dd_Smed_v4_1126_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Human
Match: DYNLRB2 (dynein light chain roadblock-type 2 [Source:HGNC Symbol;Acc:HGNC:15467])

HSP 1 Score: 144.05 bits (362), Expect = 3.422e-44
Identity = 67/99 (67.68%), Postives = 82/99 (82.83%), Query Frame = 1 
Query:   91 EGKSEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASCE 387
            E ++EVEE +KRI SHKGVIG ++VN +GIPIRTTLDN+TTVQY  L+  L  KA+S V+D+DP NDLTFLRIRSKK+EIMVAPD++Y+ IVIQ   CE
Sbjct:   28 EPQAEVEETLKRIQSHKGVIGTMVVNAEGIPIRTTLDNSTTVQYAGLLHHLTMKAKSTVRDIDPQNDLTFLRIRSKKHEIMVAPDKEYLLIVIQNP-CE 125
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Human
Match: DYNLRB2 (dynein light chain roadblock-type 2 [Source:HGNC Symbol;Acc:HGNC:15467])

HSP 1 Score: 142.124 bits (357), Expect = 8.644e-44
Identity = 66/96 (68.75%), Postives = 80/96 (83.33%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASCE 387
            +EVEE +KRI SHKGVIG ++VN +GIPIRTTLDN+TTVQY  L+  L  KA+S V+D+DP NDLTFLRIRSKK+EIMVAPD++Y+ IVIQ   CE
Sbjct:    2 AEVEETLKRIQSHKGVIGTMVVNAEGIPIRTTLDNSTTVQYAGLLHHLTMKAKSTVRDIDPQNDLTFLRIRSKKHEIMVAPDKEYLLIVIQNP-CE 96
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Human
Match: DYNLRB1 (dynein light chain roadblock-type 1 [Source:HGNC Symbol;Acc:HGNC:15468])

HSP 1 Score: 131.724 bits (330), Expect = 8.115e-40
Identity = 61/94 (64.89%), Postives = 75/94 (79.79%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEAS 381
            +EVEE +KR+ S KGV G I+VNT+GIPI++T+DN TT QY +L+     KARS V+D+DP NDLTFLRIRSKKNEIMVAPD+DY  IVIQ  +
Sbjct:    2 AEVEETLKRLQSQKGVQGIIVVNTEGIPIKSTMDNPTTTQYASLMHSFILKARSTVRDIDPQNDLTFLRIRSKKNEIMVAPDKDYFLIVIQNPT 95
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Human
Match: DYNLRB1 (dynein light chain roadblock-type 1 [Source:HGNC Symbol;Acc:HGNC:15468])

HSP 1 Score: 131.724 bits (330), Expect = 4.220e-39
Identity = 61/92 (66.30%), Postives = 75/92 (81.52%), Query Frame = 1 
Query:   97 KSEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQ 372
            ++EVEE +KR+ S KGV G I+VNT+GIPI++T+DN TT QY +L+     KARS V+D+DP NDLTFLRIRSKKNEIMVAPD+DY  IVIQ
Sbjct:   53 EAEVEETLKRLQSQKGVQGIIVVNTEGIPIKSTMDNPTTTQYASLMHSFILKARSTVRDIDPQNDLTFLRIRSKKNEIMVAPDKDYFLIVIQ 144
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Celegans
Match: dyrb-1 (Dynein light chain roadblock [Source:UniProtKB/TrEMBL;Acc:Q22752])

HSP 1 Score: 100.908 bits (250), Expect = 5.126e-28
Identity = 44/94 (46.81%), Postives = 71/94 (75.53%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEAS 381
            S+ EE I+R+ S KGV+G I+V++ G  I +T+D+  T  + A + QL  K ++++++LD  NDLTFLR+R+KKNEIM+APD+D++ +VI++ S
Sbjct:    2 SDFEETIRRLQSEKGVVGIIVVDSAGRVIHSTIDSDATQSHTAFLQQLCEKTKTSIRELDSSNDLTFLRLRTKKNEIMIAPDKDHVIMVIKDLS 95
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Fly
Match: robl (gene:FBgn0024196 transcript:FBtr0086920)

HSP 1 Score: 142.124 bits (357), Expect = 4.315e-44
Identity = 63/90 (70.00%), Postives = 78/90 (86.67%), Query Frame = 1 
Query:  103 EVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQ 372
            EVEE +KRI SHKGV+G I+VN +GIP+++TLDN TTVQY  L++QL  KARS V+DLDP ND+TFLR+RSKK+EIMVAPD+D+I IVIQ
Sbjct:    4 EVEETLKRIQSHKGVVGTIVVNNEGIPVKSTLDNTTTVQYAGLMSQLADKARSVVRDLDPSNDMTFLRVRSKKHEIMVAPDKDFILIVIQ 93
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Fly
Match: robl22E (gene:FBgn0028570 transcript:FBtr0331451)

HSP 1 Score: 101.679 bits (252), Expect = 3.257e-28
Identity = 46/91 (50.55%), Postives = 63/91 (69.23%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQ 372
            +EVEE +KR  S K V G ++V+ DGIPI+TTLD   T+ Y AL+  +  KAR  V DLD  N+ TFLR+R+++NE+M+ P  DY  +VIQ
Sbjct:    3 AEVEELLKRFQSMKNVTGIVVVDNDGIPIKTTLDYTLTLHYAALMQTVREKARQVVLDLDATNEFTFLRLRTEQNEVMLCPQEDYFIMVIQ 93
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Fly
Match: robl22E (gene:FBgn0028570 transcript:FBtr0077800)

HSP 1 Score: 101.679 bits (252), Expect = 3.257e-28
Identity = 46/91 (50.55%), Postives = 63/91 (69.23%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQ 372
            +EVEE +KR  S K V G ++V+ DGIPI+TTLD   T+ Y AL+  +  KAR  V DLD  N+ TFLR+R+++NE+M+ P  DY  +VIQ
Sbjct:    3 AEVEELLKRFQSMKNVTGIVVVDNDGIPIKTTLDYTLTLHYAALMQTVREKARQVVLDLDATNEFTFLRLRTEQNEVMLCPQEDYFIMVIQ 93
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Fly
Match: CG10834 (gene:FBgn0032972 transcript:FBtr0085943)

HSP 1 Score: 78.9518 bits (193), Expect = 2.379e-19
Identity = 37/91 (40.66%), Postives = 60/91 (65.93%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQ 372
            +E+E+ +KR  ++  V G II++   IPI+TT++   TV Y ALI+ L  KA   + +LD  N+L  +R+R+K +E++V P  +YI IV+Q
Sbjct:    3 AEIEDLLKRYQNYPNVSGIIILDPFAIPIKTTMEYTLTVHYAALISTLTYKAAKMITNLDASNELVTIRLRTKVHEVIVLPSENYIIIVVQ 93
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Fly
Match: CG10822 (gene:FBgn0034478 transcript:FBtr0086339)

HSP 1 Score: 77.411 bits (189), Expect = 1.438e-18
Identity = 33/98 (33.67%), Postives = 58/98 (59.18%), Query Frame = 1 
Query:   85 PKEGKSEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEA 378
            PK  KS VEE  +++    GV   +I+N  G+P++T++D    +QY  L   L  K ++ +  ++P  +LT LR+R+K +E+++ PD     +V+Q A
Sbjct:    8 PKRTKSYVEEVFRKVQEKPGVEDILIMNHSGVPVKTSMDRQEGLQYACLYDNLREKCQAFLSKMEPAQNLTLLRVRTKYHEVLITPDAKITVLVVQNA 105
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Zebrafish
Match: dynlrb2 (dynein light chain roadblock-type 2 [Source:ZFIN;Acc:ZDB-GENE-120709-101])

HSP 1 Score: 135.576 bits (340), Expect = 3.346e-41
Identity = 62/94 (65.96%), Postives = 78/94 (82.98%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEAS 381
            +EVE+ +KRI +  GVIG I+VN +GIPIRTTLDN+T+VQY  L+ QL  KARSAV+D+DP N+LTFLRIRSKK+EIMVAPD++Y+ I IQ  S
Sbjct:   18 AEVEDTLKRIQAQHGVIGTIVVNGEGIPIRTTLDNSTSVQYAGLLHQLTMKARSAVRDIDPQNELTFLRIRSKKHEIMVAPDKEYLLIAIQNPS 111
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Zebrafish
Match: CR788324.3 (pep chromosome:GRCz11:CHR_ALT_CTG25_1_17:21894675:21903448:1 gene:ENSDARG00000113449.1 transcript:ENSDART00000188897.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR788324.3)

HSP 1 Score: 132.88 bits (333), Expect = 2.458e-40
Identity = 61/92 (66.30%), Postives = 76/92 (82.61%), Query Frame = 1 
Query:  106 VEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEAS 381
            VE+ +KRI +  GVIG I+VN +GIPIRTTLDN+T+VQY  L+ QL  KARSAV+D+DP N+LTFLRIRSKK+EIMVAPD++Y+ I IQ  S
Sbjct:    4 VEDTLKRIQAQHGVIGTIVVNGEGIPIRTTLDNSTSVQYAGLLHQLTMKARSAVRDIDPQNELTFLRIRSKKHEIMVAPDKEYLLIAIQNPS 95
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Zebrafish
Match: dynlrb1 (dynein, light chain, roadblock-type 1 [Source:ZFIN;Acc:ZDB-GENE-040426-989])

HSP 1 Score: 132.494 bits (332), Expect = 2.802e-40
Identity = 64/94 (68.09%), Postives = 75/94 (79.79%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEAS 381
            +EVEE IKRI S KGV G IIVN +GIPI++TLDN +TVQY A I QL  KAR  V+D+DP NDLTFLR+RSKKNEIM+APD+DY  IVIQ  +
Sbjct:    2 AEVEETIKRIQSQKGVQGIIIVNAEGIPIKSTLDNTSTVQYAANIHQLLMKARGIVRDIDPQNDLTFLRVRSKKNEIMIAPDKDYFLIVIQNPT 95
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Zebrafish
Match: CR788324.3 (pep chromosome:GRCz11:CHR_ALT_CTG25_1_17:21894675:21900573:1 gene:ENSDARG00000113449.1 transcript:ENSDART00000184632.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR788324.3)

HSP 1 Score: 119.013 bits (297), Expect = 4.733e-35
Identity = 55/79 (69.62%), Postives = 67/79 (84.81%), Query Frame = 1 
Query:  106 VEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAP 342
            VE+ +KRI +  GVIG I+VN +GIPIRTTLDN+T+VQY  L+ QL  KARSAV+D+DP N+LTFLRIRSKK+EIMVAP
Sbjct:    4 VEDTLKRIQAQHGVIGTIVVNGEGIPIRTTLDNSTSVQYAGLLHQLTMKARSAVRDIDPQNELTFLRIRSKKHEIMVAP 82
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Zebrafish
Match: dynlrb1 (dynein, light chain, roadblock-type 1 [Source:ZFIN;Acc:ZDB-GENE-040426-989])

HSP 1 Score: 70.0922 bits (170), Expect = 2.233e-16
Identity = 31/44 (70.45%), Postives = 37/44 (84.09%), Query Frame = 1 
Query:  250 KARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEAS 381
            KAR  V+D+DP NDLTFLR+RSKKNEIM+APD+DY  IVIQ  +
Sbjct:    2 KARGIVRDIDPQNDLTFLRVRSKKNEIMIAPDKDYFLIVIQNPT 45
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Xenopus
Match: akap6 (A-kinase anchoring protein 6 [Source:Xenbase;Acc:XB-GENE-982363])

HSP 1 Score: 145.206 bits (365), Expect = 6.059e-45
Identity = 67/94 (71.28%), Postives = 81/94 (86.17%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEAS 381
            +EVEE +KRI SHKGVIG I+VN +GIPIRTTLDN+TTVQY  L+ QL+ KA+S V+D+DP NDLTFLRIRSKK+EIMVAPD++Y+ IVIQ  S
Sbjct:   13 AEVEETLKRIQSHKGVIGTIVVNAEGIPIRTTLDNSTTVQYAGLLHQLSMKAKSTVRDIDPQNDLTFLRIRSKKHEIMVAPDKEYLLIVIQNPS 106
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Xenopus
Match: akap6 (A-kinase anchoring protein 6 [Source:Xenbase;Acc:XB-GENE-982363])

HSP 1 Score: 132.109 bits (331), Expect = 4.480e-40
Identity = 61/85 (71.76%), Postives = 73/85 (85.88%), Query Frame = 1 
Query:  127 ILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEAS 381
            I SHKGVIG I+VN +GIPIRTTLDN+TTVQY  L+ QL+ KA+S V+D+DP NDLTFLRIRSKK+EIMVAPD++Y+ IVIQ  S
Sbjct:    2 IQSHKGVIGTIVVNAEGIPIRTTLDNSTTVQYAGLLHQLSMKAKSTVRDIDPQNDLTFLRIRSKKHEIMVAPDKEYLLIVIQNPS 86
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Xenopus
Match: tmc2 (transmembrane channel like 2 [Source:Xenbase;Acc:XB-GENE-6050839])

HSP 1 Score: 129.413 bits (324), Expect = 8.106e-39
Identity = 60/94 (63.83%), Postives = 77/94 (81.91%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEAS 381
            ++VEE +KRI   KGV G IIVN++GIPI++T+DN TTVQY +L+ QL  KAR +V+D+D  NDLTFLRIRSKKNEIM+APD+DY  IVIQ+ +
Sbjct:    2 ADVEETLKRIQGQKGVQGIIIVNSEGIPIKSTMDNQTTVQYASLMHQLVMKARGSVRDIDCQNDLTFLRIRSKKNEIMIAPDKDYFLIVIQQPT 95
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Mouse
Match: Dynlrb2 (dynein light chain roadblock-type 2 [Source:MGI Symbol;Acc:MGI:1922715])

HSP 1 Score: 143.665 bits (361), Expect = 1.104e-44
Identity = 67/96 (69.79%), Postives = 81/96 (84.38%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASCE 387
            +EVEE +KRI SHKGVIG ++VN +GIPIRTTLDN+TTVQY  L+ QL  KA+S V+D+DP NDLTFLRIRSKK+EIMVAPD++Y+ IVIQ   CE
Sbjct:    2 TEVEETLKRIQSHKGVIGTMVVNAEGIPIRTTLDNSTTVQYAGLLHQLTMKAKSTVRDIDPQNDLTFLRIRSKKHEIMVAPDKEYLLIVIQNP-CE 96
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Mouse
Match: Dynlrb1 (dynein light chain roadblock-type 1 [Source:MGI Symbol;Acc:MGI:1914318])

HSP 1 Score: 130.183 bits (326), Expect = 3.258e-39
Identity = 61/99 (61.62%), Postives = 76/99 (76.77%), Query Frame = 1 
Query:   85 PKEGKSEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEAS 381
            P   ++EVEE +KR+ S KGV G I+VNT+GIPI++T+DN TT QY  L+     KARS V+++DP NDLTFLRIRSKKNEIMVAPD+DY  IVIQ  +
Sbjct:    5 PPLWEAEVEETLKRLQSQKGVQGIIVVNTEGIPIKSTMDNPTTTQYANLMHNFILKARSTVREIDPQNDLTFLRIRSKKNEIMVAPDKDYFLIVIQNPT 103
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Mouse
Match: Dynlrb2 (dynein light chain roadblock-type 2 [Source:MGI Symbol;Acc:MGI:1922715])

HSP 1 Score: 129.413 bits (324), Expect = 3.270e-39
Identity = 59/82 (71.95%), Postives = 71/82 (86.59%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPD 345
            +EVEE +KRI SHKGVIG ++VN +GIPIRTTLDN+TTVQY  L+ QL  KA+S V+D+DP NDLTFLRIRSKK+EIMVAP+
Sbjct:    2 TEVEETLKRIQSHKGVIGTMVVNAEGIPIRTTLDNSTTVQYAGLLHQLTMKAKSTVRDIDPQNDLTFLRIRSKKHEIMVAPE 83
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Mouse
Match: Dynlrb1 (dynein light chain roadblock-type 1 [Source:MGI Symbol;Acc:MGI:1914318])

HSP 1 Score: 129.028 bits (323), Expect = 7.976e-39
Identity = 60/94 (63.83%), Postives = 74/94 (78.72%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEAS 381
            +EVEE +KR+ S KGV G I+VNT+GIPI++T+DN TT QY  L+     KARS V+++DP NDLTFLRIRSKKNEIMVAPD+DY  IVIQ  +
Sbjct:    2 AEVEETLKRLQSQKGVQGIIVVNTEGIPIKSTMDNPTTTQYANLMHNFILKARSTVREIDPQNDLTFLRIRSKKNEIMVAPDKDYFLIVIQNPT 95
[Close]
BLAST of Dynein light chain roadblock vs. UniProt/SwissProt
Match: sp|Q9DAJ5|DLRB2_MOUSE (Dynein light chain roadblock-type 2 OS=Mus musculus OX=10090 GN=Dynlrb2 PE=1 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 7.721e-44
Identity = 67/96 (69.79%), Postives = 81/96 (84.38%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASCE 387
            +EVEE +KRI SHKGVIG ++VN +GIPIRTTLDN+TTVQY  L+ QL  KA+S V+D+DP NDLTFLRIRSKK+EIMVAPD++Y+ IVIQ   CE
Sbjct:    2 TEVEETLKRIQSHKGVIGTMVVNAEGIPIRTTLDNSTTVQYAGLLHQLTMKAKSTVRDIDPQNDLTFLRIRSKKHEIMVAPDKEYLLIVIQNP-CE 96
[Close]
BLAST of Dynein light chain roadblock vs. UniProt/SwissProt
Match: sp|Q32P85|DLRB2_BOVIN (Dynein light chain roadblock-type 2 OS=Bos taurus OX=9913 GN=DYNLRB2 PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 8.244e-44
Identity = 67/96 (69.79%), Postives = 81/96 (84.38%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASCE 387
            +EVEE +KRI SHKGVIG ++VN +GIPIRTTLDN+TTVQY  L+ QL  KA+S V+D+DP NDLTFLRIRSKK+EIMVAPD++Y+ IVIQ   CE
Sbjct:    2 AEVEETLKRIQSHKGVIGTMVVNAEGIPIRTTLDNSTTVQYAGLLHQLTMKAKSTVRDIDPQNDLTFLRIRSKKHEIMVAPDKEYLLIVIQNP-CE 96
[Close]
BLAST of Dynein light chain roadblock vs. UniProt/SwissProt
Match: sp|Q8TF09|DLRB2_HUMAN (Dynein light chain roadblock-type 2 OS=Homo sapiens OX=9606 GN=DYNLRB2 PE=1 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 4.151e-43
Identity = 66/96 (68.75%), Postives = 80/96 (83.33%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASCE 387
            +EVEE +KRI SHKGVIG ++VN +GIPIRTTLDN+TTVQY  L+  L  KA+S V+D+DP NDLTFLRIRSKK+EIMVAPD++Y+ IVIQ   CE
Sbjct:    2 AEVEETLKRIQSHKGVIGTMVVNAEGIPIRTTLDNSTTVQYAGLLHHLTMKAKSTVRDIDPQNDLTFLRIRSKKHEIMVAPDKEYLLIVIQNP-CE 96
[Close]
BLAST of Dynein light chain roadblock vs. UniProt/SwissProt
Match: sp|Q9NP97|DLRB1_HUMAN (Dynein light chain roadblock-type 1 OS=Homo sapiens OX=9606 GN=DYNLRB1 PE=1 SV=3)

HSP 1 Score: 131.724 bits (330), Expect = 3.897e-39
Identity = 61/94 (64.89%), Postives = 75/94 (79.79%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEAS 381
            +EVEE +KR+ S KGV G I+VNT+GIPI++T+DN TT QY +L+     KARS V+D+DP NDLTFLRIRSKKNEIMVAPD+DY  IVIQ  +
Sbjct:    2 AEVEETLKRLQSQKGVQGIIVVNTEGIPIKSTMDNPTTTQYASLMHSFILKARSTVRDIDPQNDLTFLRIRSKKNEIMVAPDKDYFLIVIQNPT 95
[Close]
BLAST of Dynein light chain roadblock vs. UniProt/SwissProt
Match: sp|P62628|DLRB1_RAT (Dynein light chain roadblock-type 1 OS=Rattus norvegicus OX=10116 GN=Dynlrb1 PE=1 SV=3)

HSP 1 Score: 129.028 bits (323), Expect = 5.579e-38
Identity = 60/94 (63.83%), Postives = 74/94 (78.72%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEAS 381
            +EVEE +KR+ S KGV G I+VNT+GIPI++T+DN TT QY  L+     KARS V+++DP NDLTFLRIRSKKNEIMVAPD+DY  IVIQ  +
Sbjct:    2 AEVEETLKRLQSQKGVQGIIVVNTEGIPIKSTMDNPTTTQYANLMHNFILKARSTVREIDPQNDLTFLRIRSKKNEIMVAPDKDYFLIVIQNPT 95
[Close]
BLAST of Dynein light chain roadblock vs. TrEMBL
Match: A0A1S8WKG8 (Dynein light chain roadblock OS=Opisthorchis viverrini OX=6198 GN=X801_09293 PE=3 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 1.814e-52
Identity = 79/106 (74.53%), Postives = 92/106 (86.79%), Query Frame = 1 
Query:   70 MSRGIPKEGKSEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASCE 387
            MSRGIPKEG+SEVEE  KR+LSHKGV+GAII ++DGI IR+T+DN+TTVQYC LI QL  KARSAV+DLDP NDLTFLRIRSK++EIMVAPDRDY  IV+QE   +
Sbjct:    1 MSRGIPKEGRSEVEEIFKRLLSHKGVMGAIIASSDGIAIRSTMDNSTTVQYCGLIQQLTAKARSAVRDLDPSNDLTFLRIRSKRHEIMVAPDRDYSLIVVQETGTD 106
[Close]
BLAST of Dynein light chain roadblock vs. TrEMBL
Match: A0A4S2LR95 (Robl_LC7 domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_005404 PE=4 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 9.559e-52
Identity = 79/102 (77.45%), Postives = 91/102 (89.22%), Query Frame = 1 
Query:   70 MSRGIPKEGKSEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQE 375
            MSRGIPKEG+SEVEE  KR+LSHKGV+GAII ++DGI IR+T+DN+TTVQYC LI QL  KARSAV+DLDP NDLTFLRIRSK++EIMVAPDRDY  IV+QE
Sbjct:   49 MSRGIPKEGRSEVEEIFKRLLSHKGVMGAIIASSDGIAIRSTMDNSTTVQYCGLIQQLTAKARSAVRDLDPSNDLTFLRIRSKRHEIMVAPDRDYSLIVVQE 150
[Close]
BLAST of Dynein light chain roadblock vs. TrEMBL
Match: A0A4E0S441 (Dynein light chain roadblock OS=Fasciola hepatica OX=6192 GN=D915_000584 PE=3 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 1.548e-46
Identity = 73/96 (76.04%), Postives = 82/96 (85.42%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASCE 387
            SEVEE  KR+L+HKGVIGAII N DG+ IR+++DNATTVQYC LI QL +KARSAV+DLDP NDLTFLRIRSKKNEIMVAPDRDY  IVIQE   +
Sbjct:    2 SEVEENFKRLLAHKGVIGAIIANADGVAIRSSMDNATTVQYCGLIQQLTSKARSAVRDLDPSNDLTFLRIRSKKNEIMVAPDRDYSLIVIQETGTD 97
[Close]
BLAST of Dynein light chain roadblock vs. TrEMBL
Match: A0A5J4NE34 (Dynein light chain roadblock OS=Paragonimus westermani OX=34504 GN=DEA37_0014006 PE=3 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 8.336e-46
Identity = 72/96 (75.00%), Postives = 82/96 (85.42%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASCE 387
            SEVEE  KR+L+HKGVIGAII ++DGI IR+++DN+TTVQYC LI QL  KARSAV+DLDP NDLTFLRIRSKKNEIMVAPDRDY  IV+QE   E
Sbjct:    2 SEVEETFKRLLAHKGVIGAIIGSSDGIAIRSSMDNSTTVQYCGLIQQLTAKARSAVRDLDPSNDLTFLRIRSKKNEIMVAPDRDYSLIVVQETGTE 97
[Close]
BLAST of Dynein light chain roadblock vs. TrEMBL
Match: A0A3S5AMR9 (Robl_LC7 domain-containing protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS21084 PE=4 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 9.449e-46
Identity = 73/89 (82.02%), Postives = 82/89 (92.13%), Query Frame = 1 
Query:   70 MSRGIPKEGKSEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMV 336
            MSRGIPKEGKSEVEE +KRI +HKGVIG IIV++DGIPIRTTLDN+TTV YC+LI QL +KARSAV+DLDP NDLTFLRIRSKKNEIM+
Sbjct:    1 MSRGIPKEGKSEVEETLKRIQAHKGVIGVIIVSSDGIPIRTTLDNSTTVHYCSLIQQLTSKARSAVRDLDPSNDLTFLRIRSKKNEIMM 89
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Cavefish
Match: dynlrb2 (dynein light chain roadblock-type 2 [Source:NCBI gene;Acc:103025073])

HSP 1 Score: 145.206 bits (365), Expect = 1.072e-44
Identity = 66/94 (70.21%), Postives = 80/94 (85.11%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEAS 381
            +EVE+ +KRI +HKGVIG I+VN +GIPIRTTLDN+TTVQY  L+ QL  KARS V+D+DP NDLTFLRIRSKK+EIMVAPD++Y+ IVIQ  S
Sbjct:   51 AEVEDTLKRIQTHKGVIGTIVVNAEGIPIRTTLDNSTTVQYAGLLHQLTMKARSTVRDIDPQNDLTFLRIRSKKHEIMVAPDKEYLLIVIQNPS 144
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Cavefish
Match: dynlrb2 (dynein light chain roadblock-type 2 [Source:NCBI gene;Acc:103025073])

HSP 1 Score: 132.109 bits (331), Expect = 3.094e-40
Identity = 61/85 (71.76%), Postives = 72/85 (84.71%), Query Frame = 1 
Query:  127 ILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEAS 381
            I +HKGVIG I+VN +GIPIRTTLDN+TTVQY  L+ QL  KARS V+D+DP NDLTFLRIRSKK+EIMVAPD++Y+ IVIQ  S
Sbjct:    3 IQTHKGVIGTIVVNAEGIPIRTTLDNSTTVQYAGLLHQLTMKARSTVRDIDPQNDLTFLRIRSKKHEIMVAPDKEYLLIVIQNPS 87
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Cavefish
Match: ENSAMXT00000049115.1 (pep primary_assembly:Astyanax_mexicanus-2.0:22:15317328:15320932:-1 gene:ENSAMXG00000029379.1 transcript:ENSAMXT00000049115.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 106.686 bits (265), Expect = 1.882e-27
Identity = 48/69 (69.57%), Postives = 58/69 (84.06%), Query Frame = 1 
Query:  175 GIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEAS 381
            GIPI++TLDN++TVQY  L+ QL  KARS V+DLDP NDLTFLR+RSKKNEIM+APD+DY  IVIQ  +
Sbjct:  324 GIPIKSTLDNSSTVQYAGLLHQLMMKARSTVRDLDPQNDLTFLRVRSKKNEIMIAPDKDYFLIVIQNPT 392
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Nematostella
Match: EDO34906 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SMZ7])

HSP 1 Score: 145.976 bits (367), Expect = 5.162e-46
Identity = 68/95 (71.58%), Postives = 80/95 (84.21%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASC 384
            SEVEE +KRI  HKGVIG IIVN++GIPIRTTLDN+TTVQY  LI QL  KARS V+D+DP NDLTFLRIRSKK+EIMVAPD++Y+ +VIQ  + 
Sbjct:    2 SEVEETLKRIQGHKGVIGVIIVNSEGIPIRTTLDNSTTVQYTGLIHQLTIKARSTVRDIDPQNDLTFLRIRSKKHEIMVAPDKEYLLVVIQNPTV 96
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Nematostella
Match: EDO45898 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRW8])

HSP 1 Score: 113.235 bits (282), Expect = 3.695e-33
Identity = 54/90 (60.00%), Postives = 69/90 (76.67%), Query Frame = 1 
Query:  103 EVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQ 372
            EVEE +KRI++H GV G+II N +G+ IR+T+DN+TT  Y + I  +   ARSAV+DLDP NDL FLRIRS+K EI+VAP+ DY  IVIQ
Sbjct:    6 EVEEVLKRIINHPGVTGSIITNPEGVAIRSTMDNSTTQIYLSNIQSMTQMARSAVRDLDPLNDLKFLRIRSRKYEILVAPENDYTLIVIQ 95
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Nematostella
Match: EDO28944 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZX7])

HSP 1 Score: 107.071 bits (266), Expect = 7.879e-31
Identity = 49/91 (53.85%), Postives = 69/91 (75.82%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQ 372
            S VE  +KRI SHKGVIG+I+VN++G PIR+ LDN+TT+ Y +    L   ARS V+D+DP NDL+ LR++S+K EI++AP  +Y+ IV+Q
Sbjct:    3 SSVENILKRIQSHKGVIGSIVVNSEGTPIRSDLDNSTTLLYASNCHHLAKLARSTVRDIDPQNDLSILRVKSRKYEIIIAPYEEYMLIVVQ 93
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Nematostella
Match: EDO43024 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZX7])

HSP 1 Score: 107.071 bits (266), Expect = 7.879e-31
Identity = 49/91 (53.85%), Postives = 69/91 (75.82%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQ 372
            S VE  +KRI SHKGVIG+I+VN++G PIR+ LDN+TT+ Y +    L   ARS V+D+DP NDL+ LR++S+K EI++AP  +Y+ IV+Q
Sbjct:    3 SSVENILKRIQSHKGVIGSIVVNSEGTPIRSDLDNSTTLLYASNCHHLAKLARSTVRDIDPQNDLSILRVKSRKYEIIIAPYEEYMLIVVQ 93
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Nematostella
Match: EDO42555 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S187])

HSP 1 Score: 96.6709 bits (239), Expect = 1.649e-26
Identity = 45/92 (48.91%), Postives = 64/92 (69.57%), Query Frame = 1 
Query:   97 KSEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQ 372
            +  VE+ + RI   KGV G IIVN DG+PIR+TLDN++T+QY  +   +   ARS V+D DP NDL  LR+R++K+E+++AP+     IVIQ
Sbjct:    7 RPHVEQILDRIQQQKGVTGLIIVNQDGMPIRSTLDNSSTLQYVNMCKTMCGIARSVVRDTDPTNDLKILRVRTRKHEVIMAPENGQYLIVIQ 98
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Medaka
Match: dynlrb1 (dynein light chain roadblock-type 1 [Source:NCBI gene;Acc:101160277])

HSP 1 Score: 136.346 bits (342), Expect = 8.101e-42
Identity = 64/95 (67.37%), Postives = 76/95 (80.00%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASC 384
            ++VEE +KRI S KGV G IIVN++GIPI+TTLDN +TVQY  LI QL   ARS V++LDP NDLTFLR+RSKKNEIM+APD+DY  IVIQ  S 
Sbjct:    2 ADVEETLKRIQSQKGVQGVIIVNSEGIPIKTTLDNTSTVQYAGLIQQLVLMARSTVRELDPQNDLTFLRVRSKKNEIMIAPDKDYFLIVIQNPSA 96
[Close]
BLAST of Dynein light chain roadblock vs. Ensembl Medaka
Match: dynlrb1 (dynein light chain roadblock-type 1 [Source:NCBI gene;Acc:101160277])

HSP 1 Score: 118.627 bits (296), Expect = 6.733e-35
Identity = 56/81 (69.14%), Postives = 67/81 (82.72%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAP 342
            ++VEE +KRI S KGV G IIVN++GIPI+TTLDN +TVQY  LI QL   ARS V++LDP NDLTFLR+RSKKNEIM+AP
Sbjct:    2 ADVEETLKRIQSQKGVQGVIIVNSEGIPIKTTLDNTSTVQYAGLIQQLVLMARSTVRELDPQNDLTFLRVRSKKNEIMIAP 82
[Close]
BLAST of Dynein light chain roadblock vs. Planmine SMEST
Match: SMESG000072361.1 (SMESG000072361.1)

HSP 1 Score: 217.238 bits (552), Expect = 8.364e-74
Identity = 106/106 (100.00%), Postives = 106/106 (100.00%), Query Frame = 1 
Query:   70 MSRGIPKEGKSEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASCE 387
            MSRGIPKEGKSEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASCE
Sbjct:    1 MSRGIPKEGKSEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASCE 106
[Close]
BLAST of Dynein light chain roadblock vs. Planmine SMEST
Match: SMESG000072361.1 (SMESG000072361.1)

HSP 1 Score: 196.438 bits (498), Expect = 1.227e-65
Identity = 96/96 (100.00%), Postives = 96/96 (100.00%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASCE 387
            SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASCE
Sbjct:    2 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEASCE 97
[Close]
BLAST of Dynein light chain roadblock vs. Planmine SMEST
Match: SMESG000011794.1 (SMESG000011794.1)

HSP 1 Score: 118.627 bits (296), Expect = 5.769e-35
Identity = 51/93 (54.84%), Postives = 72/93 (77.42%), Query Frame = 1 
Query:  100 SEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEA 378
            SEV+E + RI SHKG+IG +I+N DG+PI++TL++  T+QYC+L   L  K + AV++LDP NDLT +RIRS  NE+M+ P+ D+IFIVIQ+ 
Sbjct:    2 SEVDEFMTRIQSHKGIIGIVILNNDGVPIKSTLEDNETIQYCSLFHHLIYKTKMAVQELDPTNDLTLMRIRSLNNEVMIVPEHDFIFIVIQKV 94
[Close]
BLAST of Dynein light chain roadblock vs. Planmine SMEST
Match: SMESG000011794.1 (SMESG000011794.1)

HSP 1 Score: 110.923 bits (276), Expect = 4.986e-32
Identity = 47/85 (55.29%), Postives = 66/85 (77.65%), Query Frame = 1 
Query:  124 RILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQEA 378
            RI SHKG+IG +I+N DG+PI++TL++  T+QYC+L   L  K + AV++LDP NDLT +RIRS  NE+M+ P+ D+IFIVIQ+ 
Sbjct:    3 RIQSHKGIIGIVILNNDGVPIKSTLEDNETIQYCSLFHHLIYKTKMAVQELDPTNDLTLMRIRSLNNEVMIVPEHDFIFIVIQKV 87
[Close]
BLAST of Dynein light chain roadblock vs. Planmine SMEST
Match: SMESG000061103.1 (SMESG000061103.1)

HSP 1 Score: 103.605 bits (257), Expect = 6.114e-29
Identity = 48/93 (51.61%), Postives = 66/93 (70.97%), Query Frame = 1 
Query:   94 GKSEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQYCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVIQ 372
              SEVE  ++RI +HK V+G +I+N D IP+RT LDN+ T Q   +   L  +A+S V+D+DP N+LTFLR+RS+KNEIMV+P   Y  IV+Q
Sbjct:   12 SNSEVEMCLERIRTHKNVVGWLILNADYIPVRTCLDNSATQQLSGVFIPLVKRAQSVVRDIDPSNELTFLRVRSRKNEIMVSPYFGYTIIVVQ 104
The following BLAST results are available for this feature:
BLAST of Dynein light chain roadblock vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
DYNLRB23.422e-4467.68dynein light chain roadblock-type 2 [Source:HGNC S... [more]
DYNLRB28.644e-4468.75dynein light chain roadblock-type 2 [Source:HGNC S... [more]
DYNLRB18.115e-4064.89dynein light chain roadblock-type 1 [Source:HGNC S... [more]
DYNLRB14.220e-3966.30dynein light chain roadblock-type 1 [Source:HGNC S... [more]
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BLAST of Dynein light chain roadblock vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
dyrb-15.126e-2846.81Dynein light chain roadblock [Source:UniProtKB/Tr... [more]
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BLAST of Dynein light chain roadblock vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
robl4.315e-4470.00gene:FBgn0024196 transcript:FBtr0086920[more]
robl22E3.257e-2850.55gene:FBgn0028570 transcript:FBtr0331451[more]
robl22E3.257e-2850.55gene:FBgn0028570 transcript:FBtr0077800[more]
CG108342.379e-1940.66gene:FBgn0032972 transcript:FBtr0085943[more]
CG108221.438e-1833.67gene:FBgn0034478 transcript:FBtr0086339[more]
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BLAST of Dynein light chain roadblock vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dynlrb23.346e-4165.96dynein light chain roadblock-type 2 [Source:ZFIN;A... [more]
CR788324.32.458e-4066.30pep chromosome:GRCz11:CHR_ALT_CTG25_1_17:21894675:... [more]
dynlrb12.802e-4068.09dynein, light chain, roadblock-type 1 [Source:ZFIN... [more]
CR788324.34.733e-3569.62pep chromosome:GRCz11:CHR_ALT_CTG25_1_17:21894675:... [more]
dynlrb12.233e-1670.45dynein, light chain, roadblock-type 1 [Source:ZFIN... [more]
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BLAST of Dynein light chain roadblock vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
akap66.059e-4571.28A-kinase anchoring protein 6 [Source:Xenbase;Acc:X... [more]
akap64.480e-4071.76A-kinase anchoring protein 6 [Source:Xenbase;Acc:X... [more]
tmc28.106e-3963.83transmembrane channel like 2 [Source:Xenbase;Acc:X... [more]
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BLAST of Dynein light chain roadblock vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Dynlrb21.104e-4469.79dynein light chain roadblock-type 2 [Source:MGI Sy... [more]
Dynlrb13.258e-3961.62dynein light chain roadblock-type 1 [Source:MGI Sy... [more]
Dynlrb23.270e-3971.95dynein light chain roadblock-type 2 [Source:MGI Sy... [more]
Dynlrb17.976e-3963.83dynein light chain roadblock-type 1 [Source:MGI Sy... [more]
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BLAST of Dynein light chain roadblock vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9DAJ5|DLRB2_MOUSE7.721e-4469.79Dynein light chain roadblock-type 2 OS=Mus musculu... [more]
sp|Q32P85|DLRB2_BOVIN8.244e-4469.79Dynein light chain roadblock-type 2 OS=Bos taurus ... [more]
sp|Q8TF09|DLRB2_HUMAN4.151e-4368.75Dynein light chain roadblock-type 2 OS=Homo sapien... [more]
sp|Q9NP97|DLRB1_HUMAN3.897e-3964.89Dynein light chain roadblock-type 1 OS=Homo sapien... [more]
sp|P62628|DLRB1_RAT5.579e-3863.83Dynein light chain roadblock-type 1 OS=Rattus norv... [more]
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BLAST of Dynein light chain roadblock vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S8WKG81.814e-5274.53Dynein light chain roadblock OS=Opisthorchis viver... [more]
A0A4S2LR959.559e-5277.45Robl_LC7 domain-containing protein OS=Opisthorchis... [more]
A0A4E0S4411.548e-4676.04Dynein light chain roadblock OS=Fasciola hepatica ... [more]
A0A5J4NE348.336e-4675.00Dynein light chain roadblock OS=Paragonimus wester... [more]
A0A3S5AMR99.449e-4682.02Robl_LC7 domain-containing protein OS=Protopolysto... [more]
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BLAST of Dynein light chain roadblock vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
dynlrb21.072e-4470.21dynein light chain roadblock-type 2 [Source:NCBI g... [more]
dynlrb23.094e-4071.76dynein light chain roadblock-type 2 [Source:NCBI g... [more]
ENSAMXT00000049115.11.882e-2769.57pep primary_assembly:Astyanax_mexicanus-2.0:22:153... [more]
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BLAST of Dynein light chain roadblock vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dynein light chain roadblock vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dynein light chain roadblock vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO349065.162e-4671.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO458983.695e-3360.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO289447.879e-3153.85Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO430247.879e-3153.85Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO425551.649e-2648.91Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Dynein light chain roadblock vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
dynlrb18.101e-4267.37dynein light chain roadblock-type 1 [Source:NCBI g... [more]
dynlrb16.733e-3569.14dynein light chain roadblock-type 1 [Source:NCBI g... [more]
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BLAST of Dynein light chain roadblock vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000072361.18.364e-74100.00SMESG000072361.1[more]
SMESG000072361.11.227e-65100.00SMESG000072361.1[more]
SMESG000011794.15.769e-3554.84SMESG000011794.1[more]
SMESG000011794.14.986e-3255.29SMESG000011794.1[more]
SMESG000061103.16.114e-2951.61SMESG000061103.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001875 ID=SMED30001875|Name=Dynein light chain roadblock|organism=Schmidtea mediterranea sexual|type=transcript|length=532bp
TTCAATTTGTTTAAAAGATTGTTTTTCAAGAATGTTTTCATTATTTTTAA
GTAACTTTGACAACAAATAATGAGTAGAGGTATCCCTAAAGAAGGGAAGT
CGGAAGTAGAGGAAGCCATTAAAAGAATTCTTAGTCATAAAGGAGTAATT
GGGGCTATTATAGTAAACACTGATGGAATTCCAATTCGTACAACGCTAGA
TAATGCTACTACTGTTCAGTATTGTGCATTAATTACTCAACTGAATACAA
AAGCTAGAAGTGCAGTAAAGGATTTAGATCCAGGAAATGATTTGACATTT
CTACGAATTCGGAGTAAGAAAAACGAAATTATGGTGGCACCTGATCGTGA
TTATATCTTTATTGTTATTCAAGAAGCCTCATGTGAATGAAATATAATTT
GGTATTGATTGATATATATATATTGGTGTGGAATTTTTTGAGTGGTAAGC
AATGATAATGAATTGTGTTTGAAAATAATATATTGGGTTGATTTCTTTCT
AATTTACTAAAATAAAATTTATAAAATGTCTG
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protein sequence of SMED30001875-orf-1

>SMED30001875-orf-1 ID=SMED30001875-orf-1|Name=SMED30001875-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=107bp
MSRGIPKEGKSEVEEAIKRILSHKGVIGAIIVNTDGIPIRTTLDNATTVQ
YCALITQLNTKARSAVKDLDPGNDLTFLRIRSKKNEIMVAPDRDYIFIVI
QEASCE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR004942Roadblock/LAMTOR2_dom
IPR016561DYNLRB1/2
Vocabulary: cellular component
TermDefinition
GO:0005868cytoplasmic dynein complex
GO:0005737cytoplasm
GO:0005856cytoskeleton
GO:0005874microtubule
GO:0030286dynein complex
GO:0070062extracellular exosome
Vocabulary: biological process
TermDefinition
GO:0007018microtubule-based movement
GO:0006810transport
Vocabulary: molecular function
TermDefinition
GO:0003774motor activity
GO:0005515protein binding
GO:0045505dynein intermediate chain binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004942Roadblock/LAMTOR2 domainSMARTSM00960Robl_LC7_a_2coord: 13..101
e-value: 1.2E-21
score: 87.9
IPR004942Roadblock/LAMTOR2 domainPFAMPF03259Robl_LC7coord: 13..101
e-value: 1.1E-23
score: 82.8
NoneNo IPR availableGENE3DG3DSA:3.30.450.30coord: 5..104
e-value: 3.8E-42
score: 144.4
NoneNo IPR availablePANTHERPTHR10779DYNEIN LIGHT CHAIN ROADBLOCKcoord: 10..104
NoneNo IPR availablePANTHERPTHR10779:SF22DYNEIN LIGHT CHAIN ROADBLOCK-TYPE 2coord: 10..104
NoneNo IPR availableSUPERFAMILYSSF103196Roadblock/LC7 domaincoord: 11..103
IPR016561Dynein light chain roadblock-type 1/2PIRSFPIRSF009998DLC7coord: 10..105
e-value: 6.9E-40
score: 132.9