SMED30001871

Overview
NameSMED30001871
Smed IDSMED30001871
Length (bp)4222
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30001871 (SMED30001871) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30001871 (SMED30001871) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30001871 (SMED30001871) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30001871 (SMED30001871) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30001871

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior region of the whole animalSMED30001871SMESG000045185.1 SMESG000045184.1 dd_Smed_v4_4812_2_1dd_Smed_v4PMID:27063937
Scimone et al., 2016		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30001871SMESG000045185.1 SMESG000045184.1 dd_Smed_v4_4812_2_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30001871SMESG000045185.1 SMESG000045184.1 dd_Smed_v4_4812_2_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30001871SMESG000045185.1 SMESG000045184.1 dd_Smed_v4_4812_2_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30001871SMESG000045185.1 SMESG000045184.1 dd_Smed_v4_4812_2_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30001871SMESG000045184.1 SMESG000045185.1 OX_Smed_1.0.13109ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30001871 vs. Ensembl Human
Match: LPCAT1 (lysophosphatidylcholine acyltransferase 1 [Source:HGNC Symbol;Acc:HGNC:25718])

HSP 1 Score: 90.8929 bits (224), Expect = 4.510e-18
Identity = 42/87 (48.28%), Postives = 61/87 (70.11%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2373
            +LW+TL QFH +VE+EFLP Y PS EEK NP+L+A+NVR +MAE L +   DY++EDC+      +  LP    ++EF+++V+ L L
Sbjct:  212 ILWLTLCQFHNQVEIEFLPVYSPSEEEKRNPALYASNVRRVMAEALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGL 298
[Close]
BLAST of SMED30001871 vs. Ensembl Human
Match: LPCAT1 (lysophosphatidylcholine acyltransferase 1 [Source:HGNC Symbol;Acc:HGNC:25718])

HSP 1 Score: 90.8929 bits (224), Expect = 4.510e-18
Identity = 42/87 (48.28%), Postives = 61/87 (70.11%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2373
            +LW+TL QFH +VE+EFLP Y PS EEK NP+L+A+NVR +MAE L +   DY++EDC+      +  LP    ++EF+++V+ L L
Sbjct:  212 ILWLTLCQFHNQVEIEFLPVYSPSEEEKRNPALYASNVRRVMAEALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGL 298
[Close]
BLAST of SMED30001871 vs. Ensembl Human
Match: LPCAT1 (lysophosphatidylcholine acyltransferase 1 [Source:HGNC Symbol;Acc:HGNC:25718])

HSP 1 Score: 90.8929 bits (224), Expect = 6.081e-18
Identity = 42/87 (48.28%), Postives = 61/87 (70.11%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2373
            +LW+TL QFH +VE+EFLP Y PS EEK NP+L+A+NVR +MAE L +   DY++EDC+      +  LP    ++EF+++V+ L L
Sbjct:  257 ILWLTLCQFHNQVEIEFLPVYSPSEEEKRNPALYASNVRRVMAEALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGL 343
[Close]
BLAST of SMED30001871 vs. Ensembl Human
Match: LPCAT1 (lysophosphatidylcholine acyltransferase 1 [Source:HGNC Symbol;Acc:HGNC:25718])

HSP 1 Score: 90.8929 bits (224), Expect = 6.081e-18
Identity = 42/87 (48.28%), Postives = 61/87 (70.11%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2373
            +LW+TL QFH +VE+EFLP Y PS EEK NP+L+A+NVR +MAE L +   DY++EDC+      +  LP    ++EF+++V+ L L
Sbjct:  257 ILWLTLCQFHNQVEIEFLPVYSPSEEEKRNPALYASNVRRVMAEALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGL 343
[Close]
BLAST of SMED30001871 vs. Ensembl Human
Match: LPCAT2 (lysophosphatidylcholine acyltransferase 2 [Source:HGNC Symbol;Acc:HGNC:26032])

HSP 1 Score: 84.7297 bits (208), Expect = 5.035e-16
Identity = 45/89 (50.56%), Postives = 57/89 (64.04%), Query Frame = -2 
Query: 2113 CMLLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2379
            CML   T  Q  T+VE+EF+P   P+ EEK +P LFAN VR LMAE L IP  D++YEDCR M  A +  LP    +VEF+K+ + L L
Sbjct:  270 CML---TFCQLFTKVEVEFMPVQVPNDEEKNDPVLFANKVRNLMAEALGIPVTDHTYEDCRLMISAGQLTLPMEAGLVEFTKISRKLKL 355
[Close]
BLAST of SMED30001871 vs. Ensembl Fly
Match: LPCAT (gene:FBgn0052699 transcript:FBtr0110850)

HSP 1 Score: 88.5817 bits (218), Expect = 1.545e-17
Identity = 46/133 (34.59%), Postives = 74/133 (55.64%), Query Frame = -2 
Query: 1975 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLLVTIFCNYTICRKMYTERNYCLCHYIYLFYFILTTFRRGNLRQLYII 2373
            LLW+T+TQF+ R E+E+LP Y PS +E  + +L+ANNVR +MA+ L +PT DYS+ED   M+ A+   +PF   IVE  + ++ L L     +  +C+      N      I     +    +  +L +L+ +
Sbjct:  281 LLWLTMTQFYNRCEIEYLPVYTPSEDEVADANLYANNVREVMAKALGVPTSDYSFEDVIVMSRARDMKIPFPGDIVEIERTIEKLGLNESQRDAELCKGFLRLSNTDRLDIITFGELLQVDLKNTDLHKLFAL 413
[Close]
BLAST of SMED30001871 vs. Ensembl Zebrafish
Match: lpcat1 (lysophosphatidylcholine acyltransferase 1 [Source:NCBI gene;Acc:555969])

HSP 1 Score: 91.6633 bits (226), Expect = 1.843e-18
Identity = 48/110 (43.64%), Postives = 70/110 (63.64%), Query Frame = -2 
Query: 2053 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNG---LPFSDIIVEFSKVVKFLLLVTIFCNYTICRKMYTERNYC 2373
            +LW+TL Q H  VE+E+LP Y PS EEK +P+LFA+NVR +MA+ L +P IDYS+EDC+   LA   G   LP    ++EF+++V+ L L T   +  +  +  + R  C
Sbjct:  251 ILWLTLCQLHNFVEIEYLPTYTPSEEEKKDPALFASNVRRIMAKALGLPIIDYSFEDCQ---LAMAKGPLRLPKHTCLLEFARLVRLLGLKTKVTDEVLQEEACSARQLC 357
[Close]
BLAST of SMED30001871 vs. Ensembl Zebrafish
Match: lpcat1 (lysophosphatidylcholine acyltransferase 1 [Source:NCBI gene;Acc:555969])

HSP 1 Score: 91.6633 bits (226), Expect = 1.843e-18
Identity = 48/110 (43.64%), Postives = 70/110 (63.64%), Query Frame = -2 
Query: 2053 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNG---LPFSDIIVEFSKVVKFLLLVTIFCNYTICRKMYTERNYC 2373
            +LW+TL Q H  VE+E+LP Y PS EEK +P+LFA+NVR +MA+ L +P IDYS+EDC+   LA   G   LP    ++EF+++V+ L L T   +  +  +  + R  C
Sbjct:  251 ILWLTLCQLHNFVEIEYLPTYTPSEEEKKDPALFASNVRRIMAKALGLPIIDYSFEDCQ---LAMAKGPLRLPKHTCLLEFARLVRLLGLKTKVTDEVLQEEACSARQLC 357
[Close]
BLAST of SMED30001871 vs. Ensembl Zebrafish
Match: lpcat2 (lysophosphatidylcholine acyltransferase 2 [Source:NCBI gene;Acc:553683])

HSP 1 Score: 67.3958 bits (163), Expect = 8.054e-11
Identity = 35/75 (46.67%), Postives = 47/75 (62.67%), Query Frame = -2 
Query: 2113 VEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2337
            VE+EFLP   P+  EK  P  FA +VR +MA+ LK+P  D++YEDCR M  A    LP    +VEF+K+ + L L
Sbjct:  263 VEVEFLPPQVPTEMEKKCPLKFAQSVRAVMAKSLKLPVTDHTYEDCRLMIAAGELTLPMEAGLVEFTKISRKLEL 337
[Close]
BLAST of SMED30001871 vs. Ensembl Zebrafish
Match: lpcat2 (lysophosphatidylcholine acyltransferase 2 [Source:NCBI gene;Acc:553683])

HSP 1 Score: 67.3958 bits (163), Expect = 8.054e-11
Identity = 35/75 (46.67%), Postives = 47/75 (62.67%), Query Frame = -2 
Query: 2113 VEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2337
            VE+EFLP   P+  EK  P  FA +VR +MA+ LK+P  D++YEDCR M  A    LP    +VEF+K+ + L L
Sbjct:  263 VEVEFLPPQVPTEMEKKCPLKFAQSVRAVMAKSLKLPVTDHTYEDCRLMIAAGELTLPMEAGLVEFTKISRKLEL 337
[Close]
BLAST of SMED30001871 vs. Ensembl Zebrafish
Match: lpcat4 (lysophosphatidylcholine acyltransferase 4 [Source:ZFIN;Acc:ZDB-GENE-030131-5777])

HSP 1 Score: 63.5438 bits (153), Expect = 1.318e-9
Identity = 28/56 (50.00%), Postives = 36/56 (64.29%), Query Frame = -2 
Query: 2203 LWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYED 2370
            LW T +Q ++ + +EFLP Y PS EEK NP L+A NV+ LMA  L +P  DY  E 
Sbjct:  240 LWHTTSQIYSSITVEFLPVYTPSAEEKQNPELYAENVQKLMARALGVPATDYMMEG 295
[Close]
BLAST of SMED30001871 vs. Ensembl Xenopus
Match: lgi2 (leucine-rich repeat LGI family member 2 [Source:Xenbase;Acc:XB-GENE-995134])

HSP 1 Score: 86.6557 bits (213), Expect = 1.183e-16
Identity = 41/87 (47.13%), Postives = 60/87 (68.97%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2373
            +LW++L QFH  VE+EFLP Y PS EEK NP+L+ANNVR +MA+ L +   DY++EDC+      +  LP    ++EF+++V+ L L
Sbjct:  257 ILWLSLCQFHNYVEIEFLPIYTPSEEEKKNPALYANNVRRVMAKALGVSVTDYTFEDCQLALAEGQLRLPSDTSLLEFARLVRSLGL 343
[Close]
BLAST of SMED30001871 vs. Ensembl Xenopus
Match: ackr4 (atypical chemokine receptor 4 [Source:Xenbase;Acc:XB-GENE-6258927])

HSP 1 Score: 82.0333 bits (201), Expect = 3.159e-15
Identity = 44/87 (50.57%), Postives = 56/87 (64.37%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2373
            LL +TL Q  T V +EFLP Y PS EEK +P L+A+NVR  MA+ L +P  D++YEDCR M  A    LP    +VEF+K+ K L L
Sbjct:  243 LLLLTLCQICTNVTVEFLPVYVPSEEEKKDPFLYAHNVRHTMAQALGLPVTDHTYEDCRLMMTAGELTLPMETGLVEFTKISKKLNL 329
[Close]
BLAST of SMED30001871 vs. Ensembl Xenopus
Match: ackr4 (atypical chemokine receptor 4 [Source:Xenbase;Acc:XB-GENE-6258927])

HSP 1 Score: 82.0333 bits (201), Expect = 3.582e-15
Identity = 44/87 (50.57%), Postives = 56/87 (64.37%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2373
            LL +TL Q  T V +EFLP Y PS EEK +P L+A+NVR  MA+ L +P  D++YEDCR M  A    LP    +VEF+K+ K L L
Sbjct:  257 LLLLTLCQICTNVTVEFLPVYVPSEEEKKDPFLYAHNVRHTMAQALGLPVTDHTYEDCRLMMTAGELTLPMETGLVEFTKISKKLNL 343
[Close]
BLAST of SMED30001871 vs. Ensembl Xenopus
Match: ackr4 (atypical chemokine receptor 4 [Source:Xenbase;Acc:XB-GENE-6258927])

HSP 1 Score: 82.0333 bits (201), Expect = 3.719e-15
Identity = 44/87 (50.57%), Postives = 56/87 (64.37%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2373
            LL +TL Q  T V +EFLP Y PS EEK +P L+A+NVR  MA+ L +P  D++YEDCR M  A    LP    +VEF+K+ K L L
Sbjct:  272 LLLLTLCQICTNVTVEFLPVYVPSEEEKKDPFLYAHNVRHTMAQALGLPVTDHTYEDCRLMMTAGELTLPMETGLVEFTKISKKLNL 358
[Close]
BLAST of SMED30001871 vs. Ensembl Xenopus
Match: ackr4 (atypical chemokine receptor 4 [Source:Xenbase;Acc:XB-GENE-6258927])

HSP 1 Score: 81.6481 bits (200), Expect = 4.135e-15
Identity = 44/87 (50.57%), Postives = 56/87 (64.37%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2373
            LL +TL Q  T V +EFLP Y PS EEK +P L+A+NVR  MA+ L +P  D++YEDCR M  A    LP    +VEF+K+ K L L
Sbjct:  285 LLLLTLCQICTNVTVEFLPVYVPSEEEKKDPFLYAHNVRHTMAQALGLPVTDHTYEDCRLMMTAGELTLPMETGLVEFTKISKKLNL 371
[Close]
BLAST of SMED30001871 vs. Ensembl Mouse
Match: Lpcat2b (lysophosphatidylcholine acyltransferase 2B [Source:MGI Symbol;Acc:MGI:1918152])

HSP 1 Score: 87.0409 bits (214), Expect = 6.418e-17
Identity = 46/89 (51.69%), Postives = 58/89 (65.17%), Query Frame = -2 
Query: 2113 CMLLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2379
            CML   TL+Q  TRVE+EF+P Y PS EEK +P LFAN VR+ MA  LK+P  D+S EDC+ M  A    LP    +VEF+K+ + L L
Sbjct:  266 CML---TLSQLFTRVEVEFMPVYIPSEEEKKDPILFANTVRIKMANALKLPVTDHSLEDCKLMISAGALQLPMEAGLVEFTKISQKLKL 351
[Close]
BLAST of SMED30001871 vs. Ensembl Mouse
Match: Lpcat1 (lysophosphatidylcholine acyltransferase 1 [Source:MGI Symbol;Acc:MGI:2384812])

HSP 1 Score: 85.8853 bits (211), Expect = 1.312e-16
Identity = 40/87 (45.98%), Postives = 60/87 (68.97%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2373
            +LW+TL QF  +VE+EFLP Y PS EEK NP+L+A+NVR +MA+ L +   DY++EDC+      +  LP    ++EF+++V+ L L
Sbjct:  209 ILWLTLCQFQNQVEIEFLPVYCPSEEEKRNPALYASNVRRVMAKALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGL 295
[Close]
BLAST of SMED30001871 vs. Ensembl Mouse
Match: Lpcat1 (lysophosphatidylcholine acyltransferase 1 [Source:MGI Symbol;Acc:MGI:2384812])

HSP 1 Score: 85.5001 bits (210), Expect = 1.842e-16
Identity = 40/87 (45.98%), Postives = 60/87 (68.97%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2373
            +LW+TL QF  +VE+EFLP Y PS EEK NP+L+A+NVR +MA+ L +   DY++EDC+      +  LP    ++EF+++V+ L L
Sbjct:  257 ILWLTLCQFQNQVEIEFLPVYCPSEEEKRNPALYASNVRRVMAKALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGL 343
[Close]
BLAST of SMED30001871 vs. Ensembl Mouse
Match: Lpcat2 (lysophosphatidylcholine acyltransferase 2 [Source:MGI Symbol;Acc:MGI:3606214])

HSP 1 Score: 83.9593 bits (206), Expect = 4.857e-16
Identity = 42/84 (50.00%), Postives = 56/84 (66.67%), Query Frame = -2 
Query: 2113 MTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2364
            +T  Q  T+VE+EF+P   PS EEK +P LFA+ +R LMAE L+IP  D++YEDCR M  A +  LP    +VEFSK+ + L L
Sbjct:  232 LTFCQLFTKVEIEFMPVQAPSEEEKNDPVLFASRIRNLMAEALEIPVTDHTYEDCRLMISAGQLTLPMEAGLVEFSKISRKLKL 315
[Close]
BLAST of SMED30001871 vs. Ensembl Mouse
Match: Lpcat2 (lysophosphatidylcholine acyltransferase 2 [Source:MGI Symbol;Acc:MGI:3606214])

HSP 1 Score: 83.9593 bits (206), Expect = 6.468e-16
Identity = 42/84 (50.00%), Postives = 56/84 (66.67%), Query Frame = -2 
Query: 2113 MTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2364
            +T  Q  T+VE+EF+P   PS EEK +P LFA+ +R LMAE L+IP  D++YEDCR M  A +  LP    +VEFSK+ + L L
Sbjct:  272 LTFCQLFTKVEIEFMPVQAPSEEEKNDPVLFASRIRNLMAEALEIPVTDHTYEDCRLMISAGQLTLPMEAGLVEFSKISRKLKL 355
[Close]
BLAST of SMED30001871 vs. UniProt/SwissProt
Match: sp|Q1LWG4|PCAT1_DANRE (Lysophosphatidylcholine acyltransferase 1 OS=Danio rerio OX=7955 GN=lpcat1 PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.391e-17
Identity = 48/110 (43.64%), Postives = 70/110 (63.64%), Query Frame = -2 
Query: 2053 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNG---LPFSDIIVEFSKVVKFLLLVTIFCNYTICRKMYTERNYC 2373
            +LW+TL Q H  VE+E+LP Y PS EEK +P+LFA+NVR +MA+ L +P IDYS+EDC+   LA   G   LP    ++EF+++V+ L L T   +  +  +  + R  C
Sbjct:  251 ILWLTLCQLHNFVEIEYLPTYTPSEEEKKDPALFASNVRRIMAKALGLPIIDYSFEDCQ---LAMAKGPLRLPKHTCLLEFARLVRLLGLKTKVTDEVLQEEACSARQLC 357
[Close]
BLAST of SMED30001871 vs. UniProt/SwissProt
Match: sp|Q8NF37|PCAT1_HUMAN (Lysophosphatidylcholine acyltransferase 1 OS=Homo sapiens OX=9606 GN=LPCAT1 PE=1 SV=2)

HSP 1 Score: 90.8929 bits (224), Expect = 2.920e-17
Identity = 42/87 (48.28%), Postives = 61/87 (70.11%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2373
            +LW+TL QFH +VE+EFLP Y PS EEK NP+L+A+NVR +MAE L +   DY++EDC+      +  LP    ++EF+++V+ L L
Sbjct:  257 ILWLTLCQFHNQVEIEFLPVYSPSEEEKRNPALYASNVRRVMAEALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGL 343
[Close]
BLAST of SMED30001871 vs. UniProt/SwissProt
Match: sp|Q0KHU5|PCAT_DROME (Lysophosphatidylcholine acyltransferase OS=Drosophila melanogaster OX=7227 GN=LPCAT PE=2 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.549e-16
Identity = 46/133 (34.59%), Postives = 74/133 (55.64%), Query Frame = -2 
Query: 1975 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLLVTIFCNYTICRKMYTERNYCLCHYIYLFYFILTTFRRGNLRQLYII 2373
            LLW+T+TQF+ R E+E+LP Y PS +E  + +L+ANNVR +MA+ L +PT DYS+ED   M+ A+   +PF   IVE  + ++ L L     +  +C+      N      I     +    +  +L +L+ +
Sbjct:  281 LLWLTMTQFYNRCEIEYLPVYTPSEDEVADANLYANNVREVMAKALGVPTSDYSFEDVIVMSRARDMKIPFPGDIVEIERTIEKLGLNESQRDAELCKGFLRLSNTDRLDIITFGELLQVDLKNTDLHKLFAL 413
[Close]
BLAST of SMED30001871 vs. UniProt/SwissProt
Match: sp|Q9D5U0|PCT2B_MOUSE (Lysophosphatidylcholine acyltransferase 2B OS=Mus musculus OX=10090 GN=Lpcat2b PE=2 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 4.489e-16
Identity = 46/89 (51.69%), Postives = 58/89 (65.17%), Query Frame = -2 
Query: 2113 CMLLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2379
            CML   TL+Q  TRVE+EF+P Y PS EEK +P LFAN VR+ MA  LK+P  D+S EDC+ M  A    LP    +VEF+K+ + L L
Sbjct:  266 CML---TLSQLFTRVEVEFMPVYIPSEEEKKDPILFANTVRIKMANALKLPVTDHSLEDCKLMISAGALQLPMEAGLVEFTKISQKLKL 351
[Close]
BLAST of SMED30001871 vs. UniProt/SwissProt
Match: sp|Q3TFD2|PCAT1_MOUSE (Lysophosphatidylcholine acyltransferase 1 OS=Mus musculus OX=10090 GN=Lpcat1 PE=1 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.289e-15
Identity = 40/87 (45.98%), Postives = 60/87 (68.97%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2373
            +LW+TL QF  +VE+EFLP Y PS EEK NP+L+A+NVR +MA+ L +   DY++EDC+      +  LP    ++EF+++V+ L L
Sbjct:  257 ILWLTLCQFQNQVEIEFLPVYCPSEEEKRNPALYASNVRRVMAKALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGL 343
[Close]
BLAST of SMED30001871 vs. TrEMBL
Match: A0A2J7PQW8 (Lysophosphatidylcholine acyltransferase OS=Cryptotermes secundus OX=105785 GN=LPCAT PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 2.317e-19
Identity = 55/110 (50.00%), Postives = 69/110 (62.73%), Query Frame = -2 
Query: 2110 CIILPIKLNIT-------SRCMLLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLLV 2418
            CI  P KL+             LLW+TLTQ H+  E+EFLP Y P+ EEK +P L+ANNVR LMA+ L IP  DY+Y+DCR MT AKR  LPF+  +VE  K+ + L L 
Sbjct:  227 CIRYPNKLDTVTWTWEGPGALKLLWLTLTQVHSSCEIEFLPVYTPNDEEKHDPKLYANNVRHLMAKTLGIPVSDYTYDDCRLMTKAKRMNLPFASGLVEVQKLRQKLGLT 336
[Close]
BLAST of SMED30001871 vs. TrEMBL
Match: A0A182U1F3 (EF-hand domain-containing protein OS=Anopheles melas OX=34690 PE=4 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 6.190e-19
Identity = 50/113 (44.25%), Postives = 69/113 (61.06%), Query Frame = -2 
Query: 2044 MLLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLLVTIFCNYTI--CRKMYTERNYCLCH 2376
             LLW TLTQFHT  E+EFLP Y+PS EEK NP L+A+NVR+LMA+ L IP  DY+++DC+ MT  K  G+P    I +  K+ + L +        I    + +T+ N  L +
Sbjct:   91 QLLWRTLTQFHTFCEIEFLPVYYPSEEEKANPKLYADNVRMLMAKALDIPISDYTFDDCKLMTFVKNVGMPHPAAIADIGKLRETLKIAATDRESKIVASERQFTDANSLLTY 203
[Close]
BLAST of SMED30001871 vs. TrEMBL
Match: A0A067QLE2 (PlsC domain-containing protein OS=Zootermopsis nevadensis OX=136037 GN=L798_00358 PE=4 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 1.382e-18
Identity = 52/107 (48.60%), Postives = 68/107 (63.55%), Query Frame = -2 
Query: 2119 CIILPIKLNIT-------SRCMLLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFL 2418
            CI  P KL+             LLW+TLTQ H+  E++FLP Y P+ +EK +P L+ANNVR LMA+ L IP  DY+Y+DCR MT AKR  LPF+  +VE  K+ + L
Sbjct:  266 CIRYPNKLDTVTWTWEGPGALKLLWLTLTQVHSSCEIQFLPVYTPNEDEKQDPKLYANNVRRLMAKHLGIPVSDYTYDDCRLMTKAKRMNLPFASELVEVQKLRQKL 372
[Close]
BLAST of SMED30001871 vs. TrEMBL
Match: E9FU51 (EF-hand domain-containing protein OS=Daphnia pulex OX=6669 GN=DAPPUDRAFT_310604 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 2.498e-18
Identity = 48/81 (59.26%), Postives = 61/81 (75.31%), Query Frame = -2 
Query: 2131 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKV 2373
            LLW+T+TQF+T  E+EFLP Y P+ EEK NP LFA+NVR +MA+ L++P IDYSYEDCR M  AK+ GLP S  ++E   V
Sbjct:  262 LLWLTMTQFYTYCELEFLPVYVPTEEEKCNPKLFASNVRDVMAKALQVPVIDYSYEDCRLMNKAKKLGLPPSIGLIEVQNV 342
[Close]
BLAST of SMED30001871 vs. TrEMBL
Match: A0A1W4XWD4 (lysophosphatidylcholine acyltransferase 2 isoform X3 OS=Agrilus planipennis OX=224129 GN=LOC108745174 PE=4 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.910e-18
Identity = 52/110 (47.27%), Postives = 69/110 (62.73%), Query Frame = -2 
Query: 2110 CIILPIKLNIT-------SRCMLLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLLV 2418
            CI  P KL+         S   LLW+TLTQ H+  E+EFLP Y P+ EEK +P L+ANNVR +MA+ L +PT+DY+Y+DCR M  AK   LP +  I E  K+ + L L+
Sbjct:  187 CIRYPNKLDTVTWTWEGPSALKLLWLTLTQVHSYCEIEFLPVYRPNEEEKRDPKLYANNVRRVMAKALGVPTVDYTYDDCRLMARAKEMNLPCASSIAEIRKIRQRLQLI 296
[Close]
BLAST of SMED30001871 vs. Ensembl Cavefish
Match: LPCAT1 (lysophosphatidylcholine acyltransferase 1-like [Source:NCBI gene;Acc:103025629])

HSP 1 Score: 92.4337 bits (228), Expect = 9.333e-19
Identity = 45/87 (51.72%), Postives = 60/87 (68.97%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2373
            +LW+TL QFH   E+EFLP Y PS EEK NP LFA+NVR LMA+ L++P  DYS+EDC+      +  LP    ++EF+K+V+ L L
Sbjct:  249 ILWLTLCQFHNEFEIEFLPIYTPSEEEKRNPPLFAHNVRRLMAKALEVPVTDYSFEDCQLAMAEGQFRLPVDTCLLEFAKLVRRLGL 335
[Close]
BLAST of SMED30001871 vs. Ensembl Cavefish
Match: lpcat1 (lysophosphatidylcholine acyltransferase 1 [Source:NCBI gene;Acc:103025479])

HSP 1 Score: 91.6633 bits (226), Expect = 1.347e-18
Identity = 46/90 (51.11%), Postives = 64/90 (71.11%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNG---LPFSDIIVEFSKVVKFLLL 2373
            +LW+TL Q H  +E+E+LP Y PS EEK +P+LFA+NVR +MA+ L++PT DYS+EDCR   LA   G   LP    ++EFS++V+ L L
Sbjct:  286 ILWLTLCQLHNSIEIEYLPTYTPSEEEKRDPALFASNVRCIMAKALQLPTTDYSFEDCR---LAMAKGPLCLPSHTCLLEFSRLVRRLGL 372
[Close]
BLAST of SMED30001871 vs. Ensembl Cavefish
Match: lpcat1 (lysophosphatidylcholine acyltransferase 1 [Source:NCBI gene;Acc:103025479])

HSP 1 Score: 91.6633 bits (226), Expect = 1.635e-18
Identity = 46/90 (51.11%), Postives = 64/90 (71.11%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNG---LPFSDIIVEFSKVVKFLLL 2373
            +LW+TL Q H  +E+E+LP Y PS EEK +P+LFA+NVR +MA+ L++PT DYS+EDCR   LA   G   LP    ++EFS++V+ L L
Sbjct:  286 ILWLTLCQLHNSIEIEYLPTYTPSEEEKRDPALFASNVRCIMAKALQLPTTDYSFEDCR---LAMAKGPLCLPSHTCLLEFSRLVRRLGL 372
[Close]
BLAST of SMED30001871 vs. Ensembl Cavefish
Match: lpcat1 (lysophosphatidylcholine acyltransferase 1 [Source:NCBI gene;Acc:103025479])

HSP 1 Score: 91.2781 bits (225), Expect = 1.679e-18
Identity = 46/90 (51.11%), Postives = 64/90 (71.11%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNG---LPFSDIIVEFSKVVKFLLL 2373
            +LW+TL Q H  +E+E+LP Y PS EEK +P+LFA+NVR +MA+ L++PT DYS+EDCR   LA   G   LP    ++EFS++V+ L L
Sbjct:  226 ILWLTLCQLHNSIEIEYLPTYTPSEEEKRDPALFASNVRCIMAKALQLPTTDYSFEDCR---LAMAKGPLCLPSHTCLLEFSRLVRRLGL 312
[Close]
BLAST of SMED30001871 vs. Ensembl Cavefish
Match: lpcat4 (lysophosphatidylcholine acyltransferase 4 [Source:ZFIN;Acc:ZDB-GENE-030131-5777])

HSP 1 Score: 63.1586 bits (152), Expect = 1.360e-9
Identity = 35/88 (39.77%), Postives = 48/88 (54.55%), Query Frame = -2 
Query: 2125 TSRCMLLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVK 2388
            TS    LW T +Q +T V +EFLP Y PS EE+ NP L+A+NV+ LMA+ L +P  +Y  E     T      LP      E  K+++
Sbjct:  217 TSWLECLWYTTSQLYTNVTVEFLPVYTPSQEERENPELYADNVQKLMAKALNVPATNYVMEGLFPATKLGGLSLPLESPAKETLKLLR 304
[Close]
BLAST of SMED30001871 vs. Ensembl Nematostella
Match: EDO41615 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3X7])

HSP 1 Score: 73.1738 bits (178), Expect = 3.259e-14
Identity = 36/85 (42.35%), Postives = 52/85 (61.18%), Query Frame = -2 
Query: 2119 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFL 2373
            LLW+T+ QFH  +E+E LP Y+P  EE  +  LFA NVR+ M+  L +P  ++++ED R M  A    LP S  +VE+ K+   L
Sbjct:  142 LLWLTMCQFHNFLEIEILPVYYPCTEEINDAKLFARNVRMQMSSALGVPMTEHTFEDTRLMLEATHKKLPASTGLVEYQKLSSKL 226
[Close]
BLAST of SMED30001871 vs. Ensembl Nematostella
Match: EDO47389 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RMR0])

HSP 1 Score: 69.707 bits (169), Expect = 1.720e-12
Identity = 38/85 (44.71%), Postives = 47/85 (55.29%), Query Frame = -2 
Query: 2131 SRCMLLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKV 2385
            S   LL   + Q H   E+EFLP + P+ EE  NP LFA NV+  MA+ L IPT  +S+ED   M      GLP    +VEF KV
Sbjct:  147 SGFQLLMFIMCQLHHPAEVEFLPVWQPNNEEMENPKLFAENVQNSMAKALGIPTTGHSFEDVLLMKEGSNLGLPDDAAVVEFQKV 231
[Close]
BLAST of SMED30001871 vs. Ensembl Medaka
Match: LPCAT1 (lysophosphatidylcholine acyltransferase 1 [Source:NCBI gene;Acc:101159130])

HSP 1 Score: 84.7297 bits (208), Expect = 2.526e-16
Identity = 41/87 (47.13%), Postives = 57/87 (65.52%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2373
            +LW+TL Q H    +EFLP Y PS EEK NP+LFA NVR +MA+ L +P  DYS+EDC+      +  LP    ++EF+++V+ L L
Sbjct:  236 ILWLTLCQLHNEFVIEFLPIYTPSEEEKKNPALFAVNVRRVMAQALGVPITDYSFEDCQLAMAEGQLKLPVETCLLEFARLVRRLGL 322
[Close]
BLAST of SMED30001871 vs. Ensembl Medaka
Match: LPCAT1 (lysophosphatidylcholine acyltransferase 1 [Source:NCBI gene;Acc:101159130])

HSP 1 Score: 84.3445 bits (207), Expect = 3.166e-16
Identity = 41/87 (47.13%), Postives = 57/87 (65.52%), Query Frame = -2 
Query: 2113 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2373
            +LW+TL Q H    +EFLP Y PS EEK NP+LFA NVR +MA+ L +P  DYS+EDC+      +  LP    ++EF+++V+ L L
Sbjct:  252 ILWLTLCQLHNEFVIEFLPIYTPSEEEKKNPALFAVNVRRVMAQALGVPITDYSFEDCQLAMAEGQLKLPVETCLLEFARLVRRLGL 338
[Close]
BLAST of SMED30001871 vs. Ensembl Medaka
Match: lpcat2 (lysophosphatidylcholine acyltransferase 2 [Source:NCBI gene;Acc:101172965])

HSP 1 Score: 75.8702 bits (185), Expect = 1.608e-13
Identity = 36/75 (48.00%), Postives = 51/75 (68.00%), Query Frame = -2 
Query: 2113 VEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2337
            VE+EFLP + P+ EEK +P+L+AN VR  MA+ L++P  D++YEDCR M  A    LP    +VEF+K+ + L L
Sbjct:  262 VEIEFLPPHVPTEEEKKSPALYANRVRETMAQALRVPVTDHTYEDCRLMISAGELTLPMEAGLVEFTKISRKLNL 336
[Close]
BLAST of SMED30001871 vs. Ensembl Medaka
Match: lpcat4 (lysophospholipid acyltransferase LPCAT4 [Source:NCBI gene;Acc:101161012])

HSP 1 Score: 51.9878 bits (123), Expect = 3.897e-6
Identity = 25/55 (45.45%), Postives = 36/55 (65.45%), Query Frame = -2 
Query: 2167 MEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGL 2331
            ++FLP Y PS EEK +P+L+A+NV+ LMA  L +P  DY  E    + ++K  GL
Sbjct:  249 LQFLPVYKPSEEEKKDPNLYADNVQKLMAGALGVPATDYVMEG--RVPVSKLGGL 301
[Close]
BLAST of SMED30001871 vs. Planmine SMEST
Match: SMESG000045185.1 (SMESG000045185.1)

HSP 1 Score: 370.548 bits (950), Expect = 1.723e-114
Identity = 176/178 (98.88%), Postives = 176/178 (98.88%), Query Frame = -3 
Query:  345 FYRSSLGDEEEMMKQLINTIQTYGTRKITLAEFCAKFHVPPESVSMMDAIFKFYDKAKSGSIHLRDFVSHVALYSFSHNRKLGFTYAFQAFKESSNQDDCEIYNETLRHVFMLSSDDINEVFTIIQAQYDHGSINLDTIDEGMQVIVDPFNMYKDYVYKYNWSKRKSENGSNMAPENV 878
            F  SSLGDEEEMMKQLINTIQTYGTRKITLAEFCAKFHVPPESVSMMDAIFKFYDKAKSGSIHLRDFVSHVALYSFSHNRKLGFTYAFQAFKESSNQDDCEIYNETLRHVFMLSSDDINEVFTIIQAQYDHGSINLDTIDEGMQVIVDPFNMYKDYVYKYNWSKRKSENGSNMAPENV
Sbjct:  347 FLLSSLGDEEEMMKQLINTIQTYGTRKITLAEFCAKFHVPPESVSMMDAIFKFYDKAKSGSIHLRDFVSHVALYSFSHNRKLGFTYAFQAFKESSNQDDCEIYNETLRHVFMLSSDDINEVFTIIQAQYDHGSINLDTIDEGMQVIVDPFNMYKDYVYKYNWSKRKSENGSNMAPENV 524

HSP 2 Score: 185.652 bits (470), Expect = 1.362e-49
Identity = 88/88 (100.00%), Postives = 88/88 (100.00%), Query Frame = -2 
Query: 2116 CMLLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLL 2379
            CMLLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLL
Sbjct:  262 CMLLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLL 349
[Close]
BLAST of SMED30001871 vs. Planmine SMEST
Match: SMESG000003820.1 (SMESG000003820.1)

HSP 1 Score: 120.168 bits (300), Expect = 1.151e-27
Identity = 56/86 (65.12%), Postives = 70/86 (81.40%), Query Frame = -2 
Query: 2116 LLWMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLL 2373
            LLWM   Q HT VE+EFLP Y PS EEK NP LFA+NVR +MA+ L +PT ++SYEDCR+MTLAK++GLPF++IIVEFS++ K LL
Sbjct:  269 LLWMLFAQIHTFVEIEFLPLYHPSEEEKRNPKLFADNVRAIMAKALNVPTTNHSYEDCRYMTLAKKHGLPFTEIIVEFSRLQKRLL 354

HSP 2 Score: 92.0485 bits (227), Expect = 1.206e-18
Identity = 54/155 (34.84%), Postives = 87/155 (56.13%), Query Frame = -3 
Query:  396 DEEEMMKQLINTIQTYGT-RKITLAEFCAKFHVPPESVSMMDAIFKFYDKAKSGSIHLRDFVSHVALYSFSHNRKLGFTYAFQAFKESSNQDDCEIYNETLRHVFMLSSDDINEVFTIIQAQYDHGSINLDTIDEGMQVIVDPFNMYKDYVYKYN 857
            D   M++ L+ TI   GT   ++L E C   ++  ++ ++++ IFKFYDK  +G I +++   H++LYS   NRK+   Y  +A++ S  +D   I  + L+  FMLSSD+  E+ +  +     G   L+T+D  M  I  PFNMY DY  +YN
Sbjct:  359 DPISMLEWLLETINRIGTDGMVSLQELCHHIYMT-DNEALVERIFKFYDKTNTGFIDIKNLFCHLSLYSLPFNRKIALDYILKAYRLSDLKDG-NIAGQVLKKSFMLSSDEAMEIISFTKFHQSPGQPFLNTLDTIMS-IGGPFNMYSDYHSEYN 510
[Close]
BLAST of SMED30001871 vs. Planmine SMEST
Match: SMESG000009198.1 (SMESG000009198.1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.991e-16
Identity = 42/85 (49.41%), Postives = 55/85 (64.71%), Query Frame = -2 
Query: 2113 WMTLTQFHTRVEMEFLPAYFPSPEEKFNPSLFANNVRLLMAEKLKIPTIDYSYEDCRHMTLAKRNGLPFSDIIVEFSKVVKFLLL 2367
            WM L QFH R  +E+LP Y P+ +EK +  LF NNVR  MA  L +P  D+S+ED + M LAK+  LP +  IVEFSK+ + L L
Sbjct:  267 WMMLCQFHNRCIVEYLPVYVPNEKEKHDAKLFGNNVRDQMARSLDVPVTDHSFEDVKLMWLAKKYKLPQAAGIVEFSKLSRKLTL 351
The following BLAST results are available for this feature:
BLAST of SMED30001871 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
LPCAT14.510e-1848.28lysophosphatidylcholine acyltransferase 1 [Source:... [more]
LPCAT14.510e-1848.28lysophosphatidylcholine acyltransferase 1 [Source:... [more]
LPCAT16.081e-1848.28lysophosphatidylcholine acyltransferase 1 [Source:... [more]
LPCAT16.081e-1848.28lysophosphatidylcholine acyltransferase 1 [Source:... [more]
LPCAT25.035e-1650.56lysophosphatidylcholine acyltransferase 2 [Source:... [more]
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BLAST of SMED30001871 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001871 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
LPCAT1.545e-1734.59gene:FBgn0052699 transcript:FBtr0110850[more]
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BLAST of SMED30001871 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lpcat11.843e-1843.64lysophosphatidylcholine acyltransferase 1 [Source:... [more]
lpcat11.843e-1843.64lysophosphatidylcholine acyltransferase 1 [Source:... [more]
lpcat28.054e-1146.67lysophosphatidylcholine acyltransferase 2 [Source:... [more]
lpcat28.054e-1146.67lysophosphatidylcholine acyltransferase 2 [Source:... [more]
lpcat41.318e-950.00lysophosphatidylcholine acyltransferase 4 [Source:... [more]
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BLAST of SMED30001871 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lgi21.183e-1647.13leucine-rich repeat LGI family member 2 [Source:Xe... [more]
ackr43.159e-1550.57atypical chemokine receptor 4 [Source:Xenbase;Acc:... [more]
ackr43.582e-1550.57atypical chemokine receptor 4 [Source:Xenbase;Acc:... [more]
ackr43.719e-1550.57atypical chemokine receptor 4 [Source:Xenbase;Acc:... [more]
ackr44.135e-1550.57atypical chemokine receptor 4 [Source:Xenbase;Acc:... [more]
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BLAST of SMED30001871 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Lpcat2b6.418e-1751.69lysophosphatidylcholine acyltransferase 2B [Source... [more]
Lpcat11.312e-1645.98lysophosphatidylcholine acyltransferase 1 [Source:... [more]
Lpcat11.842e-1645.98lysophosphatidylcholine acyltransferase 1 [Source:... [more]
Lpcat24.857e-1650.00lysophosphatidylcholine acyltransferase 2 [Source:... [more]
Lpcat26.468e-1650.00lysophosphatidylcholine acyltransferase 2 [Source:... [more]
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BLAST of SMED30001871 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q1LWG4|PCAT1_DANRE1.391e-1743.64Lysophosphatidylcholine acyltransferase 1 OS=Danio... [more]
sp|Q8NF37|PCAT1_HUMAN2.920e-1748.28Lysophosphatidylcholine acyltransferase 1 OS=Homo ... [more]
sp|Q0KHU5|PCAT_DROME1.549e-1634.59Lysophosphatidylcholine acyltransferase OS=Drosoph... [more]
sp|Q9D5U0|PCT2B_MOUSE4.489e-1651.69Lysophosphatidylcholine acyltransferase 2B OS=Mus ... [more]
sp|Q3TFD2|PCAT1_MOUSE1.289e-1545.98Lysophosphatidylcholine acyltransferase 1 OS=Mus m... [more]
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BLAST of SMED30001871 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2J7PQW82.317e-1950.00Lysophosphatidylcholine acyltransferase OS=Cryptot... [more]
A0A182U1F36.190e-1944.25EF-hand domain-containing protein OS=Anopheles mel... [more]
A0A067QLE21.382e-1848.60PlsC domain-containing protein OS=Zootermopsis nev... [more]
E9FU512.498e-1859.26EF-hand domain-containing protein OS=Daphnia pulex... [more]
A0A1W4XWD42.910e-1847.27lysophosphatidylcholine acyltransferase 2 isoform ... [more]
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BLAST of SMED30001871 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
LPCAT19.333e-1951.72lysophosphatidylcholine acyltransferase 1-like [So... [more]
lpcat11.347e-1851.11lysophosphatidylcholine acyltransferase 1 [Source:... [more]
lpcat11.635e-1851.11lysophosphatidylcholine acyltransferase 1 [Source:... [more]
lpcat11.679e-1851.11lysophosphatidylcholine acyltransferase 1 [Source:... [more]
lpcat41.360e-939.77lysophosphatidylcholine acyltransferase 4 [Source:... [more]
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BLAST of SMED30001871 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001871 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001871 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO416153.259e-1442.35Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO473891.720e-1244.71Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30001871 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4
Match NameE-valueIdentityDescription
LPCAT12.526e-1647.13lysophosphatidylcholine acyltransferase 1 [Source:... [more]
LPCAT13.166e-1647.13lysophosphatidylcholine acyltransferase 1 [Source:... [more]
lpcat21.608e-1348.00lysophosphatidylcholine acyltransferase 2 [Source:... [more]
lpcat43.897e-645.45lysophospholipid acyltransferase LPCAT4 [Source:NC... [more]
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BLAST of SMED30001871 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000045185.11.723e-11498.88SMESG000045185.1[more]
SMESG000003820.11.151e-2765.12SMESG000003820.1[more]
SMESG000009198.11.991e-1649.41SMESG000009198.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001871 ID=SMED30001871|Name=SMED30001871|organism=Schmidtea mediterranea sexual|type=transcript|length=4222bp
GTGTACATTTATTCGTCATGTATAATATTATATCAATAATAAAAATCAAA
AACACATTTAACAAAATTACACAGTTGAAACGTAATCAAAATATAAATAC
AAACACTATACAAAAATGATACAACAAATTGCTTTGCAAGATAAATACAT
TCTTGGACTGAATCTTGCATCAATTCAAGAGCCAATTATAGGATTATCAT
ATAAATACTAAGAAATTACAATCTATGATAACTTTAAAAAATGGTTTGGT
ATAAGCCCTTGATATAAAAATAAAGAAAAATAATTGCTGTGATTTTAAGT
TAAGCTAACAAAAACTCTCAAATAACTCATAAAATGAAATTTTATACATT
TTCTGGTGCCATGTTGGAGCCATTTTCACTTTTTCTTTTACTCCAATTGT
ATTTATACACATAATCCTTGTACATGTTGAAAGGGTCTACAATTACTTGC
ATTCCTTCATCAATTGTATCTAAATTAATGCTGCCATGGTCATATTGGGC
TTGGATTATGGTAAAAACTTCATTTATATCATCAGAACTAAGCATAAACA
CATGTCGAAGTGTTTCATTGTAAATCTCACAATCATCTTGATTTGAAGAT
TCTTTGAATGCTTGAAATGCATACGTAAATCCTAACTTTCGATTGTGAGA
GAAGGAATAAAGAGCAACATGAGATACGAAGTCGCGAAGGTGAATGGATC
CTGATTTAGCTTTATCGTAAAATTTAAATATCGCATCCATCATAGAAACA
GATTCAGGGGGAACATGAAATTTTGCACAAAATTCTGCCAGTGTAATTTT
TCTTGTCCCATATGTTTGAATTGTATTTATTAATTGTTTCATCATTTCTT
CTTCATCCCCCAATGAAGATCTGTAAAATGATTTATTATCACTGACATTG
CACATTAACATAAAGGTGACATTACTACTTTTTTAAATATAAAAAAATAC
TCAAAGATATGAATTTAAAATTGAAATATACCATGAAATTCTACTAATCA
ATAAATAGCACAATCATAGTTACTGTATAGTTTCAATCGCGCCTTAAATT
AATGAAGTAAAATTTATATATTAAAATCAATTAAATATATTTATGACTGG
CAGTAATGTCAGAACTACAAAATGTCTCAGAAATTGTGAAATTGTGTAAG
GCACCGAATTTGCACCGCTCCAAAAACCCATTCGTTCTATGGTAATTTCT
TAAACCTTTATGAACCTAGCCTTCAATCGAAATTATAAAGATGGTTAATA
ATCGAAATAATTTAAAAGAAAAAGTTTTCTACACCTTAAAATGGAAACCC
CCCTCTCTAAAGAATCTAAACATCCCAAATCAATTTTTGGATCTACAGAA
TTTTAAGAAGCCGAATTTATTACAAATTCAAAGACTTTTCGAATCGAAAC
AAATCGAATACTTAATGATCCTTATTTTGACGATTCTAGCTAGTTTTTTT
ATAAAATTTTATTTAGTTTCACTTCAAATTTATTTGTTAATTTCAAAATC
TTATAAAATGTCCAAAAGTGAAACGATTAAAATAACCGTTGAATTTTTCT
TTTCTAGATTTGTTTTCATTGACATAAACATGTATAAGATTTTGTCAGTA
AATTAAATCGCAAATTAATGAAATTTTAAATATGAAAAATAATACAGTGA
TAACTGCGATTCATCTCTGTAAAAGACAAAATATTTTTAATGTTAGAATC
AAATACAAAAAGCCGAAATAAGATTTCGGAAGAATTGTTAATACAACTGC
GAGTTGAGTTCGAAATAGGTTATTGTCGTTCAAAATTTTCTTTGGAAGGA
TTTTGTTCCTTGAATTAAAATTATTATAAAACTAATATCTAGATAAATCC
CATGGCTGCGGTGTGAGAGGGCATTTCTTCATTAGTTCAGTGAATGAGTA
ATTTAAGGATAAAACCATTTAATAATTATAATTTAACGATTTAATAATAA
AAAAAATTGAATTTATGTAATTCTAATTATATAGAGTTGTCGTAAATTTC
CACGCCTAAAAGTTGTAAGGATAAAATAAAAAAGATATATGTAATGACAT
AAGCAGTAATTCCTCTCTGTGTACATCTTTCGACATATGGTATAATTACA
AAAAATCGTCACTAACAATAAAAATTTTACGACTTTACTGAATTCCACAA
TTATATCTGAAAAAGGCAATCCATTTCGCTTTGCCAATGTCATATGGCGG
CAATCTTCATACGAATAGTCAATAGTAGGAATCTTTAACTTTTCAGCCAT
CAATAACCGCACGTTATTAGCAAATAAAGATGGATTGAATTTTTCTTCCG
GACTGGGAAAATAAGCAGGTAAAAACTCCATTTCCACCCTTGTATGGAAT
TGAGTCAAAGTCATCCATAACAACATACATCTAGAGGTAATATTTAATTT
AATTGGTAAAATAATACAAGTATTCATTTAATATAAATAATCCCATTTAG
AATCGTGCTTAATCTTTGGGGTTCGGAGAAAACAATTATTAAGGAGTGGA
CATAGTGACAATAATTGTTAAGCTTTCGTGTTTGTAATATGAAATTATAA
ATTTGATCCCACTGCATTATGCACTCACAGAACTCATCATAAACTGGAAA
CCGCGTCGAGTGTATGACATAAGTATTGTCACATCTACCTTTACAACTAA
TCACACGCCAGTGATCAATAATTTGGTTAAAACGTACACGTACTTTCATA
TTCTGAGTACAAATGTCAACGAGACAAAAAGACCGATAAACGAACGATAA
TTTCCTTAGCTTTTTGCAATAAACGTAATTTACTAACACAAAATCGGAAT
CACAGTTTTCAAGGGGTTGAGAGAACATAGTTTGAAATTAAATGAAGAGA
AATGCAAAATTAGTCAACTAACTTGTTATTTTACATTTAAAGTATTTTTA
TTAATTTATAACGATTATGATTGTTTTCATATTCATACCCCAATGCATCA
TGTATGATGACATAATCACCTCTCAAAATATATAGCGCCAAAATTTACAA
ACAAGCAAATTATTAGCCTAAGAACTTCTAGTGTTGAGGGTTTTGGTTTT
AAAAAACGAGTTATTAATACAGTAAAATTTCTATTACACAGTACTATATT
TTCAAATATTTAGCCATAAACTTGGAACTAGGACTGCATTATTTGTTTTA
GAGTCAATGTTGATGAGATTGATAGACGATTGTTTGAGTTTTTTTATTGA
TCATCCTAATACTTTCAAATTAGTGATTGACATTCAGGGTCTTTGGATCC
AGAATAAATCGCCTCGCTCAAGGACAGGAAGAATTTTCGAATTCGATATG
CCTTGATTAGTATTGCATGTGTAACATTATTTTATACTTATTAAGAACTT
AGTATTATTAAGAACTTTAATACAACTTACAACTTAATACAACTTATATA
CAACTTAGTACAACACCATAAAAAGATTCACAAATAAAATTGAATTTAAA
TGTTTTATTTGAACTCCAAAAATACAGGATTTCACCAAACTTAACATTTT
GTGACTTCTGCAGAATTTAATTACATTTATAAAATTTTAAAGAAATGAAA
TAAAATTCATACAATTTTGTTTATATATGAAAGAAATTATATGAAAGTAA
TTTCTGAAGGGCCCTTAAATGACCACAGTGAAAATACTAAATATTGCAAA
ATGGTAAATGTTACTTGCTAGCCAATATATCAACTATTAATTATTAGAAA
TAAATTACATCTACGCTAGCAAAAACAGAACTAGGTTAAAAGTAAACATA
AGCTACCAAAGCTGCAATAAGTTGAAACAAAACTAACAGAAAATATAATT
TAGTAAATATTCAACAGTTACAAAAATAAAACATCCCGATCTTACTTGTG
GTTCGCTAAAATTTACTGAAAACCAATTTAAAACATAATTAATTGGACAG
GTAATATATGAATTTATTTATACAACAATGCAAAAAATTGCACACGATTC
TAGAAATTAAGAAAAATCCACTATCATCCGAATATTATACTAGTCAAACT
GTATAATATTCTTATTTACAAGTCTCGACCTATTATTACGGTGGTCGGAC
AAAATGGCACGAACAAAATGATAAAAAATTGTACTTTAGAATTCTTACGA
ATGAGATAAAAACTGCAATATAATTAAATCATTTTCCCATGAAATAAGCA
ATGACAACTATTTAAACAGTAACAATGAACCTAATATTCGTTTGGGTTTC
AAATTAAAAACGTAGTTATAAA
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protein sequence of SMED30001871-orf-1

>SMED30001871-orf-1 ID=SMED30001871-orf-1|Name=SMED30001871-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=190bp
MLMCNVSDNKSFYRSSLGDEEEMMKQLINTIQTYGTRKITLAEFCAKFHV
PPESVSMMDAIFKFYDKAKSGSIHLRDFVSHVALYSFSHNRKLGFTYAFQ
AFKESSNQDDCEIYNETLRHVFMLSSDDINEVFTIIQAQYDHGSINLDTI
DEGMQVIVDPFNMYKDYVYKYNWSKRKSENGSNMAPENV*
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protein sequence of SMED30001871-orf-2

>SMED30001871-orf-2 ID=SMED30001871-orf-2|Name=SMED30001871-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=122bp
MVNNRNNLKEKVFYTLKWKPPSLKNLNIPNQFLDLQNFKKPNLLQIQRLF
ESKQIEYLMILILTILASFFIKFYLVSLQIYLLISKSYKMSKSETIKITV
EFFFSRFVFIDINMYKILSVN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000099neuron
PLANA:0000142posterior region of the whole animal
PLANA:0000418head
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 2..154
e-value: 5.2E-7
score: 31.5
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 53..88
score: 9.464
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 16..93