SMED30001834

Overview
NameSMED30001834
Smed IDSMED30001834
Length (bp)1070
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30001834 (SMED30001834) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30001834 (SMED30001834) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30001834 (SMED30001834) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30001834 (SMED30001834) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30001834

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 23

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30001834SMESG000017098.1 Contig702GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30001834SMESG000068935.1 Contig702GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
head regionSMED30001834SMESG000068935.1 SmedASXL_012945SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30001834SMESG000068935.1 dd_Smed_v4_357_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30001834SMESG000068935.1 dd_Smed_v4_357_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30001834SMESG000068935.1 dd_Smed_v4_357_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001834SMESG000068935.1 Contig29398newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001834SMESG000068935.1 Contig29398uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001834SMESG000068935.1 Contig22580newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001834SMESG000068935.1 Contig22580uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001834SMESG000068935.1 Contig43594newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001834SMESG000068935.1 Contig9917newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001834SMESG000068935.1 Contig9917uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001834SMESG000068935.1 Contig27150newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001834SMESG000068935.1 Contig27150uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30001834SMESG000068935.1 dd_357dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
head regionSMED30001834SMESG000068935.1 dd_Smed_v6_357_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30001834SMESG000068935.1 OX_Smed_1.0.08964ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30001834SMESG000068935.1 OX_Smed_1.0.18450ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30001834SMESG000068935.1 dd_Smed_v6_357_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30001834SMESG000068935.1 dd_Smed_v4_357_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
dorsal region of the epidermisSMED30001834SMESG000068935.1 dd_Smed_v4_357_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30001834SMESG000068935.1 dd_Smed_v4_357_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30001834 vs. Planmine SMEST
Match: SMESG000068971.1 (SMESG000068971.1)

HSP 1 Score: 247.669 bits (631), Expect = 1.108e-74
Identity = 144/302 (47.68%), Postives = 189/302 (62.58%), Query Frame = 1 
Query:  130 FPIILTNTAQPGEFSASLILQLNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSM-------------------SNGSVKAVYTLEVAMNGIMTDESLSNNFINATNSAYSSAVIINNXXXXXXXXXXXXXXXXXXXXXXXKA-------------DPVKPGEHSFAVAMICLFTDEVYTTDYNNPTSAKYIDFKGRQFVKWQAALNAKLIRLIEIIINSLTSGSVKADMSLNVASTSALSTTDIQNTVTQAAQAAYGNSLQILSISAKQTSAAPKTQSWLATAFL 939
            +P IL N AQPGE+SAS+ L + F N  YIQ FDN+ SPET NFFNEYYRNI+S L+  KIR+ SAV+NSM                   ++  + +  +LE+A++  M  E+L+++F N T + + +A   NN  +       T   A  TTT+S  A             DP +  E S+ + + C FTDE Y  DY  PTSA+YI+FK R   KW+AAL  KL+RLIEIIINSL+SGSVKADM +N+AS S++ST D+QNTVTQAAQAAYG+SLQ+ SI+A    A+   QSW+A A L
Sbjct:  528 WPFILNNPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSMRFVYNYNIYIDFMDKNYRNASADIYSNISLELAISATMDSETLTSSFNNVTFTVFMTACNYNNYFLATILKSPTLVAANQTTTVSLTATTSLPITTLSIIEDPPRKNERSYKIIIKCSFTDETYYDDYKTPTSARYINFKARHHAKWKAALIFKLLRLIEIIINSLSSGSVKADMEMNIASNSSISTADLQNTVTQAAQAAYGSSLQVFSITATSLDASSTDQSWIAAAVL 829

HSP 2 Score: 120.168 bits (300), Expect = 1.607e-29
Identity = 76/224 (33.93%), Postives = 118/224 (52.68%), Query Frame = 1 
Query:  130 FPIILTNTAQPGEFSASLILQLNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSM-------------------SNGSVKAVYTLEVAMNGIMTDESLSNNFINATNSAYSSAVIINNXXXXXXXXXXXXXXXXXXXXXXXKADPVKPGEHSFAVAMICLFTDEVYTTDYNNPTSAKYIDFKGRQFVKWQAALNAKLIRLIEIIINSL 744
            +P IL N AQPGE+SAS+ L + F N  YIQ FDN+ SPET NFFNEYYRNI+S L+  KIR+ SAV+NSM                   ++  + +  +LE+A++  M  E+L+++F N T + + +AVII N   +                     +P +PGE+S ++ ++  F +  Y   ++N  S +  +F    +    + L    IR+   +INS+
Sbjct:  260 WPFILNNPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSMRFVYNYNIYIDFMDKNYRNASADIYSNISLELAISATMDSETLTSSFNNVTFTVFMTAVIITNWPFILN-------------------NPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSM 464

HSP 3 Score: 120.168 bits (300), Expect = 1.868e-29
Identity = 76/223 (34.08%), Postives = 117/223 (52.47%), Query Frame = 1 
Query:  133 PIILTNTAQPGEFSASLILQLNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSM-------------------SNGSVKAVYTLEVAMNGIMTDESLSNNFINATNSAYSSAVIINNXXXXXXXXXXXXXXXXXXXXXXXKADPVKPGEHSFAVAMICLFTDEVYTTDYNNPTSAKYIDFKGRQFVKWQAALNAKLIRLIEIIINSL 744
            P IL N AQPGE+SAS+ L + F N  YIQ FDN+ SPET NFFNEYYRNI+S L+  KIR+ SAV+NSM                   ++  + +  +LE+A++  M  E+L+++F N T + + +AVII N   +                     +P +PGE+S ++ ++  F +  Y   ++N  S +  +F    +    + L    IR+   +INS+
Sbjct:  127 PFILNNPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSMRFVYKYNIYIDFMDKNYRNASADIYSNISLELAISATMDSETLTSSFNNVTFTVFMTAVIITNWPFILN-------------------NPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSM 330

HSP 4 Score: 94.7449 bits (234), Expect = 5.909e-21
Identity = 69/203 (33.99%), Postives = 112/203 (55.17%), Query Frame = 1 
Query:  193 LNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSM-------------------SNGSVKAVYTLEVAMNGIMTDESLSNNFINATNSAYSSAVIINNXXXXXXXXXXXXXXXXXXXXXXXKADPVKPGEHSFAVAMICLFTDEVYTTDYNNPTSAKYIDFKGRQFVKWQAALNAKLIRLIEIIINSL 744
            + F N  YIQ FDN+ SPET NFFNEYYRNI+S L+  KIR+ SAV+NSM                   ++  + +  +LE+A++  M  E+L+++F N T + + +AVII NST+L++ TLV +    +        +P +PGE+S ++ ++  F +  Y   ++N  S +  +F    +    + L    IR+   +INS+
Sbjct:    1 MTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSMRFVYNYNIYIDFMDKNYRNASADIYSNISLELAISATMDSETLTSSFNNVTFTVFMTAVIITNSTILKSPTLVAARPFILN-------NPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSM 196

HSP 5 Score: 48.521 bits (114), Expect = 6.999e-6
Identity = 31/116 (26.72%), Postives = 61/116 (52.59%), Query Frame = 1 
Query:  127 SFPI----ILTNTAQPGEFSASLILQLNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSMSNGSVKAVYTLEVAMNGIMTDESLSNNFINATNSAYSSAV 462
            S PI    I+ +  +  E S  +I++ +FT+  Y   +   +S   +NF   ++    ++L+   +R++  ++NS+S+GSVKA   + +A N  ++   L N    A  +AY S++
Sbjct:  689 SLPITTLSIIEDPPRKNERSYKIIIKCSFTDETYYDDYKTPTSARYINFKARHHAKWKAALIFKLLRLIEIIINSLSSGSVKADMEMNIASNSSISTADLQNTVTQAAQAAYGSSL 804
[Close]
BLAST of SMED30001834 vs. Planmine SMEST
Match: SMESG000068971.1 (SMESG000068971.1)

HSP 1 Score: 248.054 bits (632), Expect = 1.249e-74
Identity = 144/301 (47.84%), Postives = 188/301 (62.46%), Query Frame = 1 
Query:  133 PIILTNTAQPGEFSASLILQLNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSM-------------------SNGSVKAVYTLEVAMNGIMTDESLSNNFINATNSAYSSAVIINNXXXXXXXXXXXXXXXXXXXXXXXKA-------------DPVKPGEHSFAVAMICLFTDEVYTTDYNNPTSAKYIDFKGRQFVKWQAALNAKLIRLIEIIINSLTSGSVKADMSLNVASTSALSTTDIQNTVTQAAQAAYGNSLQILSISAKQTSAAPKTQSWLATAFL 939
            P IL N AQPGE+SAS+ L + F N  YIQ FDN+ SPET NFFNEYYRNI+S L+  KIR+ SAV+NSM                   ++  + +  +LE+A++  M  E+L+++F N T + + +A   NN  +       T   A  TTT+S  A             DP +  E S+ + + C FTDE Y  DY  PTSA+YI+FK R   KW+AAL  KL+RLIEIIINSL+SGSVKADM +N+AS S++ST D+QNTVTQAAQAAYG+SLQ+ SI+A    A+   QSW+A A L
Sbjct:  553 PFILNNPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSMRFVYNYNIYIDFMDKNYRNASADIYSNISLELAISATMDSETLTSSFNNVTFTVFMTACNYNNYFLATILKSPTLVAANQTTTVSLTATTSLPITTLSIIEDPPRKNERSYKIIIKCSFTDETYYDDYKTPTSARYINFKARHHAKWKAALIFKLLRLIEIIINSLSSGSVKADMEMNIASNSSISTADLQNTVTQAAQAAYGSSLQVFSITATSLDASSTDQSWIAAAVL 853

HSP 2 Score: 120.553 bits (301), Expect = 1.182e-29
Identity = 81/224 (36.16%), Postives = 127/224 (56.70%), Query Frame = 1 
Query:  130 FPIILTNTAQPGEFSASLILQLNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSM-------------------SNGSVKAVYTLEVAMNGIMTDESLSNNFINATNSAYSSAVIINNXXXXXXXXXXXXXXXXXXXXXXXKADPVKPGEHSFAVAMICLFTDEVYTTDYNNPTSAKYIDFKGRQFVKWQAALNAKLIRLIEIIINSL 744
            +P IL N AQPGE+SAS+ L + F N  YIQ FDN+ SPET NFFNEYYRNI+S L+  KIR+ SAV+NSM                   ++  + +  +LE+A++  M  E+L+++F N T + + +AVII NST+L++ TL  +    +        +P +PGE+S ++ ++  F +  Y   ++N  S +  +F    +    + L    IR+   +INS+
Sbjct:  260 WPFILNNPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSMRFVYNYNIYIDFMDKNYRNASADIYSNISLELAISATMDSETLTSSFNNVTFTVFMTAVIITNSTILKSPTLEIARPFILN-------NPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSM 476

HSP 3 Score: 120.168 bits (300), Expect = 1.657e-29
Identity = 76/223 (34.08%), Postives = 117/223 (52.47%), Query Frame = 1 
Query:  133 PIILTNTAQPGEFSASLILQLNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSM-------------------SNGSVKAVYTLEVAMNGIMTDESLSNNFINATNSAYSSAVIINNXXXXXXXXXXXXXXXXXXXXXXXKADPVKPGEHSFAVAMICLFTDEVYTTDYNNPTSAKYIDFKGRQFVKWQAALNAKLIRLIEIIINSL 744
            P IL N AQPGE+SAS+ L + F N  YIQ FDN+ SPET NFFNEYYRNI+S L+  KIR+ SAV+NSM                   ++  + +  +LE+A++  M  E+L+++F N T + + +AVII N   +                     +P +PGE+S ++ ++  F +  Y   ++N  S +  +F    +    + L    IR+   +INS+
Sbjct:  127 PFILNNPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSMRFVYKYNIYIDFMDKNYRNASADIYSNISLELAISATMDSETLTSSFNNVTFTVFMTAVIITNWPFILN-------------------NPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSM 330

HSP 4 Score: 95.1301 bits (235), Expect = 4.444e-21
Identity = 69/203 (33.99%), Postives = 112/203 (55.17%), Query Frame = 1 
Query:  193 LNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSM-------------------SNGSVKAVYTLEVAMNGIMTDESLSNNFINATNSAYSSAVIINNXXXXXXXXXXXXXXXXXXXXXXXKADPVKPGEHSFAVAMICLFTDEVYTTDYNNPTSAKYIDFKGRQFVKWQAALNAKLIRLIEIIINSL 744
            + F N  YIQ FDN+ SPET NFFNEYYRNI+S L+  KIR+ SAV+NSM                   ++  + +  +LE+A++  M  E+L+++F N T + + +AVII NST+L++ TLV +    +        +P +PGE+S ++ ++  F +  Y   ++N  S +  +F    +    + L    IR+   +INS+
Sbjct:    1 MTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSMRFVYNYNIYIDFMDKNYRNASADIYSNISLELAISATMDSETLTSSFNNVTFTVFMTAVIITNSTILKSPTLVAARPFILN-------NPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSM 196

HSP 5 Score: 48.521 bits (114), Expect = 6.859e-6
Identity = 31/116 (26.72%), Postives = 61/116 (52.59%), Query Frame = 1 
Query:  127 SFPI----ILTNTAQPGEFSASLILQLNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSMSNGSVKAVYTLEVAMNGIMTDESLSNNFINATNSAYSSAV 462
            S PI    I+ +  +  E S  +I++ +FT+  Y   +   +S   +NF   ++    ++L+   +R++  ++NS+S+GSVKA   + +A N  ++   L N    A  +AY S++
Sbjct:  713 SLPITTLSIIEDPPRKNERSYKIIIKCSFTDETYYDDYKTPTSARYINFKARHHAKWKAALIFKLLRLIEIIINSLSSGSVKADMEMNIASNSSISTADLQNTVTQAAQAAYGSSL 828
[Close]
BLAST of SMED30001834 vs. Planmine SMEST
Match: SMESG000068971.1 (SMESG000068971.1)

HSP 1 Score: 247.669 bits (631), Expect = 1.286e-74
Identity = 144/301 (47.84%), Postives = 188/301 (62.46%), Query Frame = 1 
Query:  133 PIILTNTAQPGEFSASLILQLNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSM-------------------SNGSVKAVYTLEVAMNGIMTDESLSNNFINATNSAYSSAVIINNXXXXXXXXXXXXXXXXXXXXXXXKA-------------DPVKPGEHSFAVAMICLFTDEVYTTDYNNPTSAKYIDFKGRQFVKWQAALNAKLIRLIEIIINSLTSGSVKADMSLNVASTSALSTTDIQNTVTQAAQAAYGNSLQILSISAKQTSAAPKTQSWLATAFL 939
            P IL N AQPGE+SAS+ L + F N  YIQ FDN+ SPET NFFNEYYRNI+S L+  KIR+ SAV+NSM                   ++  + +  +LE+A++  M  E+L+++F N T + + +A   NN  +       T   A  TTT+S  A             DP +  E S+ + + C FTDE Y  DY  PTSA+YI+FK R   KW+AAL  KL+RLIEIIINSL+SGSVKADM +N+AS S++ST D+QNTVTQAAQAAYG+SLQ+ SI+A    A+   QSW+A A L
Sbjct:  541 PFILNNPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSMRFVYNYNIYIDFMDKNYRNASADIYSNISLELAISATMDSETLTSSFNNVTFTVFMTACNYNNYFLATILKSPTLVAANQTTTVSLTATTSLPITTLSIIEDPPRKNERSYKIIIKCSFTDETYYDDYKTPTSARYINFKARHHAKWKAALIFKLLRLIEIIINSLSSGSVKADMEMNIASNSSISTADLQNTVTQAAQAAYGSSLQVFSITATSLDASSTDQSWIAAAVL 841

HSP 2 Score: 120.168 bits (300), Expect = 1.431e-29
Identity = 76/224 (33.93%), Postives = 118/224 (52.68%), Query Frame = 1 
Query:  130 FPIILTNTAQPGEFSASLILQLNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSM-------------------SNGSVKAVYTLEVAMNGIMTDESLSNNFINATNSAYSSAVIINNXXXXXXXXXXXXXXXXXXXXXXXKADPVKPGEHSFAVAMICLFTDEVYTTDYNNPTSAKYIDFKGRQFVKWQAALNAKLIRLIEIIINSL 744
            +P IL N AQPGE+SAS+ L + F N  YIQ FDN+ SPET NFFNEYYRNI+S L+  KIR+ SAV+NSM                   ++  + +  +LE+A++  M  E+L+++F N T + + +AVII N   +                     +P +PGE+S ++ ++  F +  Y   ++N  S +  +F    +    + L    IR+   +INS+
Sbjct:  260 WPFILNNPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSMRFVYNYNIYIDFMDKNYRNASADIYSNISLELAISATMDSETLTSSFNNVTFTVFMTAVIITNWPFILN-------------------NPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSM 464

HSP 3 Score: 120.168 bits (300), Expect = 1.617e-29
Identity = 76/223 (34.08%), Postives = 117/223 (52.47%), Query Frame = 1 
Query:  133 PIILTNTAQPGEFSASLILQLNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSM-------------------SNGSVKAVYTLEVAMNGIMTDESLSNNFINATNSAYSSAVIINNXXXXXXXXXXXXXXXXXXXXXXXKADPVKPGEHSFAVAMICLFTDEVYTTDYNNPTSAKYIDFKGRQFVKWQAALNAKLIRLIEIIINSL 744
            P IL N AQPGE+SAS+ L + F N  YIQ FDN+ SPET NFFNEYYRNI+S L+  KIR+ SAV+NSM                   ++  + +  +LE+A++  M  E+L+++F N T + + +AVII N   +                     +P +PGE+S ++ ++  F +  Y   ++N  S +  +F    +    + L    IR+   +INS+
Sbjct:  127 PFILNNPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSMRFVYKYNIYIDFMDKNYRNASADIYSNISLELAISATMDSETLTSSFNNVTFTVFMTAVIITNWPFILN-------------------NPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSM 330

HSP 4 Score: 95.1301 bits (235), Expect = 4.716e-21
Identity = 69/203 (33.99%), Postives = 112/203 (55.17%), Query Frame = 1 
Query:  193 LNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSM-------------------SNGSVKAVYTLEVAMNGIMTDESLSNNFINATNSAYSSAVIINNXXXXXXXXXXXXXXXXXXXXXXXKADPVKPGEHSFAVAMICLFTDEVYTTDYNNPTSAKYIDFKGRQFVKWQAALNAKLIRLIEIIINSL 744
            + F N  YIQ FDN+ SPET NFFNEYYRNI+S L+  KIR+ SAV+NSM                   ++  + +  +LE+A++  M  E+L+++F N T + + +AVII NST+L++ TLV +    +        +P +PGE+S ++ ++  F +  Y   ++N  S +  +F    +    + L    IR+   +INS+
Sbjct:    1 MTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSMRFVYNYNIYIDFMDKNYRNASADIYSNISLELAISATMDSETLTSSFNNVTFTVFMTAVIITNSTILKSPTLVAARPFILN-------NPAQPGEYSASINLLMTFANRSYIQAFDNVFSPETANFFNEYYRNISSGLLLYKIRIASAVINSM 196

HSP 5 Score: 48.521 bits (114), Expect = 6.661e-6
Identity = 31/116 (26.72%), Postives = 61/116 (52.59%), Query Frame = 1 
Query:  127 SFPI----ILTNTAQPGEFSASLILQLNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSMSNGSVKAVYTLEVAMNGIMTDESLSNNFINATNSAYSSAV 462
            S PI    I+ +  +  E S  +I++ +FT+  Y   +   +S   +NF   ++    ++L+   +R++  ++NS+S+GSVKA   + +A N  ++   L N    A  +AY S++
Sbjct:  701 SLPITTLSIIEDPPRKNERSYKIIIKCSFTDETYYDDYKTPTSARYINFKARHHAKWKAALIFKLLRLIEIIINSLSSGSVKADMEMNIASNSSISTADLQNTVTQAAQAAYGSSL 816
[Close]
BLAST of SMED30001834 vs. Planmine SMEST
Match: SMESG000068935.1 (SMESG000068935.1)

HSP 1 Score: 207.994 bits (528), Expect = 3.858e-64
Identity = 150/349 (42.98%), Postives = 208/349 (59.60%), Query Frame = 1 
Query:   64 MKYFITLWFLICTSISSIKAQSFPIILTNTAQPGEFSASLILQLNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSMSNGSVKAV--YTLEVAMNGIMTDESLSNNFINATNSAYSSAVIINNXXXXXXXXXXXXXXXXXXXXXXXKAD------------------PV------KPGEHSFAVAMICLFTDEVYTTDYNNPTSAKYIDFKGRQFVKWQAALNAKLIRLIEIIINSL--TSGSVKADMSLNVASTSALSTTDIQNTVTQAAQAAYGNSLQI------------------LSISAKQTSAAPKTQSWLATAFLXXXXXXXXGLD 972
            MKYFITLWFLICTS S  +AQS P+ L+N AQPGE+SAS+ LQLNFTN  YI  + N+SSPE++N FNEY  NI+S L + KIRVVSAV+  +SNGSV  +  Y LEVA+N  M   +L ++F N T S Y +AV+I+NST+L+  TLV +         S+                     P+      +PGE+S ++ +  LF++  Y   ++N TS++ + F    +    ++L +  IR++  ++NS+   +GSVKA  ++ VA   A++   + N    A  +AY + + I                  ++ISAKQTSAAPKTQSWLATA LVPVVVVIVGLD
Sbjct:    1 MKYFITLWFLICTSTSRTEAQSPPVNLSNPAQPGEYSASIYLQLNFTNRSYIASYANVSSPESLNLFNEYNANISSGLSAYKIRVVSAVITKISNGSVFILSDYILEVAINATMDSATLVSSFNNVTFSVYMTAVVISNSTILKEPTLVGANLTTTALPASSATSVATSQITTLLTTTIIPKVPIVIGMAAQPGEYSASLYLQILFSNRTYIQAFSNVTSSESVSFFNEYYRNINSSLMSNKIRVVSAVVNSMRFVNGSVKAVYTIEVAMNGAMTDESLSNNFINATNSAYSSVVIITNSTVLQTSTLVTSTTAPITISAKQTSAAPKTQSWLATAVLVPVVVVIVGLD 349
[Close]
BLAST of SMED30001834 vs. Planmine SMEST
Match: SMESG000067844.1 (SMESG000067844.1)

HSP 1 Score: 201.83 bits (512), Expect = 4.301e-64
Identity = 103/139 (74.10%), Postives = 117/139 (84.17%), Query Frame = 1 
Query:   64 MKYFITLWFLICTSISSIKAQSFPIILTNTAQPGEFSASLILQLNFTNHLYIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSMSNGS--VKAVYTLEVAMNGIMTDESLSNNFINATNSAYSSAVIINN 474
            MKYFITLWFLICTSISSIKAQSFPIILTNTAQPGEFSASLILQLNFTNHLYIQPFDN+SS ETVNFFNEYYRNI+S L+  KIRVVSAV+NSM NGS  + +  +LEVA++  +  E+L+  F N T + Y +AVII N
Sbjct:    1 MKYFITLWFLICTSISSIKAQSFPIILTNTAQPGEFSASLILQLNFTNHLYIQPFDNVSSLETVNFFNEYYRNISSGLLLYKIRVVSAVINSMRNGSTNIYSDISLEVAISSTIDSETLTIAFNNVTFTVYMTAVIITN 139
The following BLAST results are available for this feature:
BLAST of SMED30001834 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001834 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001834 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001834 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001834 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001834 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001834 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001834 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001834 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001834 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001834 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001834 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001834 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001834 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000068971.11.108e-7447.68SMESG000068971.1[more]
SMESG000068971.11.249e-7447.84SMESG000068971.1[more]
SMESG000068971.11.286e-7447.84SMESG000068971.1[more]
SMESG000068935.13.858e-6442.98SMESG000068935.1[more]
SMESG000067844.14.301e-6474.10SMESG000067844.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001834 ID=SMED30001834|Name=SMED30001834|organism=Schmidtea mediterranea sexual|type=transcript|length=1070bp
CGGCAACTGTATAAAAGGTGAAGCCTGGCCAAGCTTTTTCAATTTTGAAT
CAGTTTTATAATAATGAAATATTTCATAACTCTTTGGTTTCTCATCTGTA
CATCAATATCGTCAATAAAAGCACAAAGTTTCCCCATTATCCTAACTAAT
ACGGCACAACCAGGAGAATTTTCCGCCTCCCTCATCTTGCAACTGAATTT
CACAAATCACTTATATATTCAACCATTTGATAATATCTCTTCACCGGAAA
CCGTGAATTTCTTCAATGAATATTACAGAAATATCAATTCAAGTTTAATG
TCGAATAAAATCCGAGTCGTCAGTGCTGTGGTTAACTCAATGAGTAACGG
AAGTGTAAAAGCTGTTTATACTCTCGAGGTCGCGATGAATGGCATAATGA
CCGACGAAAGTTTGTCAAATAATTTCATCAACGCCACAAATTCTGCGTAT
TCGTCTGCTGTAATAATTAATAATTCTACGGTCTTGCAAACTTCAACTTT
AGTGACAAGTACAACAGCTCCTATAACAACAACTATATCAACTAAAGCTG
ATCCTGTAAAACCAGGGGAACATTCCTTCGCAGTTGCTATGATATGTTTA
TTTACAGATGAAGTATATACTACTGACTATAACAATCCGACCTCCGCAAA
ATATATTGATTTTAAAGGGAGGCAATTTGTGAAATGGCAAGCGGCCCTTA
ATGCGAAATTAATAAGATTGATAGAAATTATTATCAACTCTCTAACTTCA
GGAAGTGTAAAAGCCGACATGTCATTGAATGTTGCATCCACTTCAGCTCT
TTCAACTACTGATATACAAAATACAGTAACGCAGGCAGCTCAAGCTGCCT
ATGGTAACAGTCTCCAAATACTTTCAATATCGGCTAAACAAACGAGTGCT
GCACCAAAAACTCAATCGTGGTTAGCCACTGCTTTCCTTGTTCCGGTTGT
CGTGGTCATCGTTGGATTAGATTAACACTTATTTATTTCATTTTAGTCAG
GATTTTTACAACATTATGATTACATTTGTCTTAGTATTTCAATAAAACTT
TTGAGTGTGATTAAAAAAAA
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protein sequence of SMED30001834-orf-1

>SMED30001834-orf-1 ID=SMED30001834-orf-1|Name=SMED30001834-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=304bp
MKYFITLWFLICTSISSIKAQSFPIILTNTAQPGEFSASLILQLNFTNHL
YIQPFDNISSPETVNFFNEYYRNINSSLMSNKIRVVSAVVNSMSNGSVKA
VYTLEVAMNGIMTDESLSNNFINATNSAYSSAVIINNSTVLQTSTLVTST
TAPITTTISTKADPVKPGEHSFAVAMICLFTDEVYTTDYNNPTSAKYIDF
KGRQFVKWQAALNAKLIRLIEIIINSLTSGSVKADMSLNVASTSALSTTD
IQNTVTQAAQAAYGNSLQILSISAKQTSAAPKTQSWLATAFLVPVVVVIV
GLD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000054dorsal region of the epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR000082SEA_dom