dynein assembly factor 3, axonemal

Overview
Namedynein assembly factor 3, axonemal
Smed IDSMED30001828
Length (bp)1513
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of dynein assembly factor 3, axonemal (SMED30001828) t-SNE clustered cells

Violin plots show distribution of expression levels for dynein assembly factor 3, axonemal (SMED30001828) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of dynein assembly factor 3, axonemal (SMED30001828) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for dynein assembly factor 3, axonemal (SMED30001828) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30001828

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 19

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30001828h1SMcG0020712 SmedASXL_010612SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30001828h1SMcG0020712 dd_Smed_v4_6686_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30001828h1SMcG0020712 dd_Smed_v4_6686_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30001828h1SMcG0020712 dd_Smed_v4_6686_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30001828h1SMcG0020712 dd_Smed_v4_6686_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30001828h1SMcG0020712 dd_Smed_v4_6686_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30001828h1SMcG0020712 dd_Smed_v4_6686_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30001828h1SMcG0020712 dd_Smed_v4_6686_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30001828h1SMcG0020712 dd_Smed_v4_6686_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001828h1SMcG0020712 Contig45785uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001828h1SMcG0020712 Contig45785newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001828h1SMcG0020711 Contig34002uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001828h1SMcG0020711 Contig34002newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001828h1SMcG0020712 Contig34002uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001828h1SMcG0020712 Contig34002newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001828h1SMcG0020713 Contig34002uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001828h1SMcG0020713 Contig34002newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30001828h1SMcG0020712 dd_Smed_v6_6686_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
epidermisSMED30001828h1SMcG0020712 dd_Smed_v4_6686_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Human
Match: DNAAF3 (dynein axonemal assembly factor 3 [Source:HGNC Symbol;Acc:HGNC:30492])

HSP 1 Score: 272.322 bits (695), Expect = 2.191e-84
Identity = 160/459 (34.86%), Postives = 250/459 (54.47%), Query Frame = 1
Query:   58 WWGFTPALDL-FDNELLEIDECTDK----ADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWK--DENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDAN-YDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKA 1410
            WWG +PALDL  ++  ++ D   D      ++++LL+GS D RH+L++LS      +   NF+++EN LE  AR ML   +AL    +MGLQ+++E FLE++GN L+R     F R  A  L   V         L  L  +  LKF+ERD LEA+F+ W   ++  ++F +S  WD RLR  LG+RYDA   V DWD  + LHDRGA +I+ +E+  WR  GVAF+LRD++ Y V NRTLASGR+L +  G++    GYWGDI   PF+ FGI + + +L + SN Q +  A  ++++N++E+   +A   +        A AT  +++E+                 Q+  G+P   +               +  + FLP+NS    +  K+ +   F L+Y +  ++  L   P L   + P G L+VE+ +Y+V++  +Q+  F  RV E+A+A
Sbjct:   16 WWGLSPALDLQAESPPVDPDSQADTVHSNPELDVLLLGSVDGRHLLRTLSRAKFWPRRRFNFFVLENNLEAVARHMLIFSLALEEPEKMGLQERSETFLEVWGNALLRPPVAAFVRAQADLLAHLVPEPDRLEEQLPWL-SLRALKFRERDALEAVFRFWAGGEKGPQAFPMSRLWDSRLRHYLGSRYDARRGVSDWDLRMKLHDRGAQVIHPQEFRRWRDTGVAFELRDSSAYHVPNRTLASGRLL-SYRGERVAARGYWGDIATGPFVAFGIEADDESLLRTSNGQPVKTAGEITQHNVTELLRDVAAWGR--------ARATGGDLEEQ-----------------QHAEGSPEPGTPAAPTPE--------SFTVHFLPLNS-AQTLHHKSCYNGRFQLLYVACGMVHLL--IPELGACVAPGGNLIVELARYLVDVRQEQLQGFNTRVRELAQA 436          
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Human
Match: DNAAF3 (dynein axonemal assembly factor 3 [Source:HGNC Symbol;Acc:HGNC:30492])

HSP 1 Score: 272.322 bits (695), Expect = 7.298e-84
Identity = 160/459 (34.86%), Postives = 250/459 (54.47%), Query Frame = 1
Query:   58 WWGFTPALDL-FDNELLEIDECTDK----ADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWK--DENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDAN-YDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKA 1410
            WWG +PALDL  ++  ++ D   D      ++++LL+GS D RH+L++LS      +   NF+++EN LE  AR ML   +AL    +MGLQ+++E FLE++GN L+R     F R  A  L   V         L  L  +  LKF+ERD LEA+F+ W   ++  ++F +S  WD RLR  LG+RYDA   V DWD  + LHDRGA +I+ +E+  WR  GVAF+LRD++ Y V NRTLASGR+L +  G++    GYWGDI   PF+ FGI + + +L + SN Q +  A  ++++N++E+   +A   +        A AT  +++E+                 Q+  G+P   +               +  + FLP+NS    +  K+ +   F L+Y +  ++  L   P L   + P G L+VE+ +Y+V++  +Q+  F  RV E+A+A
Sbjct:   63 WWGLSPALDLQAESPPVDPDSQADTVHSNPELDVLLLGSVDGRHLLRTLSRAKFWPRRRFNFFVLENNLEAVARHMLIFSLALEEPEKMGLQERSETFLEVWGNALLRPPVAAFVRAQADLLAHLVPEPDRLEEQLPWL-SLRALKFRERDALEAVFRFWAGGEKGPQAFPMSRLWDSRLRHYLGSRYDARRGVSDWDLRMKLHDRGAQVIHPQEFRRWRDTGVAFELRDSSAYHVPNRTLASGRLL-SYRGERVAARGYWGDIATGPFVAFGIEADDESLLRTSNGQPVKTAGEITQHNVTELLRDVAAWGR--------ARATGGDLEEQ-----------------QHAEGSPEPGTPAAPTPE--------SFTVHFLPLNS-AQTLHHKSCYNGRFQLLYVACGMVHLL--IPELGACVAPGGNLIVELARYLVDVRQEQLQGFNTRVRELAQA 483          
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Human
Match: DNAAF3 (dynein axonemal assembly factor 3 [Source:HGNC Symbol;Acc:HGNC:30492])

HSP 1 Score: 264.618 bits (675), Expect = 8.948e-81
Identity = 160/480 (33.33%), Postives = 248/480 (51.67%), Query Frame = 1
Query:   58 WWGFTPALDL----------------------FDNELLEIDECTDK----ADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWK--DENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDAN-YDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKA 1410
            WWG +PALDL                           ++ D   D      ++++LL+GS D RH+L++LS      +   NF+++EN LE  AR ML   +AL    +MGLQ+++E FLE++GN L+R     F R  A  L   V         L  L  +  LKF+ERD LEA+F+ W   ++  ++F +S  WD RLR  LG+RYDA   V DWD  + LHDRGA +I+ +E+  WR  GVAF+LRD++ Y V NRTLASGR+L +  G++    GYWGDI   PF+ FGI + + +L + SN Q +  A  ++++N++E+   +A   +        A AT  +++E+                 Q+  G+P   +               +  + FLP+NS    +  K+ +   F L+Y +  ++  L   P L   + P G L+VE+ +Y+V++  +Q+  F  RV E+A+A
Sbjct:   63 WWGLSPALDLQAERDATVDALPTTMVPQPAVILPGPPVDPDSQADTVHSNPELDVLLLGSVDGRHLLRTLSRAKFWPRRRFNFFVLENNLEAVARHMLIFSLALEEPEKMGLQERSETFLEVWGNALLRPPVAAFVRAQADLLAHLVPEPDRLEEQLPWL-SLRALKFRERDALEAVFRFWAGGEKGPQAFPMSRLWDSRLRHYLGSRYDARRGVSDWDLRMKLHDRGAQVIHPQEFRRWRDTGVAFELRDSSAYHVPNRTLASGRLL-SYRGERVAARGYWGDIATGPFVAFGIEADDESLLRTSNGQPVKTAGEITQHNVTELLRDVAAWGR--------ARATGGDLEEQ-----------------QHAEGSPEPGTPAPTPE---------SFTVHFLPLNS-AQTLHHKSCYNGRFQLLYVACGMVHLL--IPELGACVAPGGNLIVELARYLVDVRQEQLQGFNTRVRELAQA 503          
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Human
Match: DNAAF3 (dynein axonemal assembly factor 3 [Source:HGNC Symbol;Acc:HGNC:30492])

HSP 1 Score: 237.654 bits (605), Expect = 7.749e-72
Identity = 139/399 (34.84%), Postives = 215/399 (53.88%), Query Frame = 1
Query:  223 NFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWK--DENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDAN-YDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKA 1410
             F+++EN LE  AR ML   +AL    +MGLQ+++E FLE++GN L+R     F R  A  L   V         L  L  +  LKF+ERD LEA+F+ W   ++  ++F +S  WD RLR  LG+RYDA   V DWD  + LHDRGA +I+ +E+  WR  GVAF+LRD++ Y V NRTLASGR+L +  G++    GYWGDI   PF+ FGI + + +L + SN Q +  A  ++++N++E+   +A   +        A AT  +++E+                 Q+  G+P   +               +  + FLP+NS    +  K+ +   F L+Y +  ++  L   P L   + P G L+VE+ +Y+V++  +Q+  F  RV E+A+A
Sbjct:   22 QFFVLENNLEAVARHMLIFSLALEEPEKMGLQERSETFLEVWGNALLRPPVAAFVRAQADLLAHLVPEPDRLEEQLPWL-SLRALKFRERDALEAVFRFWAGGEKGPQAFPMSRLWDSRLRHYLGSRYDARRGVSDWDLRMKLHDRGAQVIHPQEFRRWRDTGVAFELRDSSAYHVPNRTLASGRLL-SYRGERVAARGYWGDIATGPFVAFGIEADDESLLRTSNGQPVKTAGEITQHNVTELLRDVAAWGR--------ARATGGDLEEQ-----------------QHAEGSPEPGTPAAPTPE--------SFTVHFLPLNS-AQTLHHKSCYNGRFQLLYVACGMVHLL--IPELGACVAPGGNLIVELARYLVDVRQEQLQGFNTRVRELAQA 382          
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Human
Match: DNAAF3 (dynein axonemal assembly factor 3 [Source:HGNC Symbol;Acc:HGNC:30492])

HSP 1 Score: 91.2781 bits (225), Expect = 5.432e-21
Identity = 49/129 (37.98%), Postives = 74/129 (57.36%), Query Frame = 1
Query:   58 WWGFTPALDL-FDNELLEIDECTDK----ADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNS 429
            WWG +PALDL  ++  ++ D   D      ++++LL+GS D RH+L++LS      +   NF+++EN LE  AR ML   +AL    +MGLQ+++E FLE++GN L+R     F R  A  L   V   
Sbjct:   16 WWGLSPALDLQAESPPVDPDSQADTVHSNPELDVLLLGSVDGRHLLRTLSRAKFWPRRRFNFFVLENNLEAVARHMLIFSLALEEPEKMGLQERSETFLEVWGNALLRPPVAAFVRAQADLLAHLVPEP 144          
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Fly
Match: CG17669 (gene:FBgn0034352 transcript:FBtr0086722)

HSP 1 Score: 182.956 bits (463), Expect = 5.127e-51
Identity = 108/327 (33.03%), Postives = 163/327 (49.85%), Query Frame = 1
Query:   61 WGFTPALDLFDNELLEIDECTDKA---------------------DINILLVGSGDSRHILQSLSSLY-KRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKY 975
            WG + ALDL++ E L   +  D                        +NIL+ G  D RH++++L+  Y  R +  +N Y+++   E+ AR ML L +AL       L  K  LF++LYGN ++R  S  +       L+K VTN       L+ +++I+ LK+KERD  E  F  W+ +    F+++  W+ R R  LGTRYD     FDWD S+TL +RG   I S+EY +WR  GVAF   +  +   N+TLA G V     G   +  GY GDI   PF GFG+ +    +    +    + A+ ++E N+ E FH++     Y
Sbjct:    3 WGISSALDLYE-EYLRAFKIKDDPLPEDEHAREEAEEAAGDNTLNTLNILICGGADPRHVIKTLAKRYTHRIRPKLNIYLLDGCAEIEARNMLLLGVALEDPESFNLVSKVHLFMDLYGNAVIRPSSHHYMAAKGRTLLKMVTNEEEL-QRLAPMLNIEGLKYKERDGFEMAFTFWQPQPWNVFEVTAYWEQRSRALLGTRYDHRNGAFDWDLSMTLKERGGQQICSQEYRYWRETGVAFVFPEYEHCKPNKTLAVGLV---RNGRNFIHRGYVGDIQTGPFCGFGLRTVEERMHHSVHGDNDYRATDITERNLMEFFHELLTQTAY 324          
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Zebrafish
Match: dnaaf3l (dynein, axonemal, assembly factor 3 like [Source:ZFIN;Acc:ZDB-GENE-150903-1])

HSP 1 Score: 281.182 bits (718), Expect = 7.013e-89
Identity = 167/481 (34.72%), Postives = 272/481 (56.55%), Query Frame = 1
Query:   37 ESLGVINWWGFTPALDLFDNELLEIDECTDKADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKAHSMQAFPVNCAAGLDKSEYVVYT 1479
            E  G + WWGF PA DL +++   +       ++N+LLVGSGD RHIL++++ L      H+  ++IEN +E+ ARQ+L L I+L+P  +M +  KTE+FLE++GN  +R+++ E  +QAA +L   +T+S    T     +    LKFKERD L  IFKLW+   S    +S  WD R+RQ+LG+RYD+    FDWD ++ LH RG  +IN  +Y+ WR  GVAF++R+  Y  +N++L S RV    G    +R GYWGDIV SP++ FGI + N  L +  NN ++  A  +SE N+ E+F  +A   +     +P  T++            +  E  + ++ +Q    A +   +       LDL+ V  VK+ FL  +S L  +  K+K+++ F+ ++ SAS++ QL++   L  I  P   L++E+  ++++++ +Q+  F  RV E+A+       PV+     +  +Y V+T
Sbjct:    8 EGAGCVTWWGFGPARDLLNSDTHRL---RPHGELNVLLVGSGDPRHILKTITGLIHSDTLHV--WVIENSMEVLARQLLLLYISLLPPEKMSVHKKTEVFLEVFGNLEIRKEAEESVKQAAAQLSISITDSLSTDTLSHSCLDTSLLKFKERDELVRIFKLWERPPSAPASVSKVWDARVRQHLGSRYDSRQGAFDWDLNMKLHQRGCGVINKHQYAKWRETGVAFEMREGLYQTANQSLLSTRVFNHRGNGVALR-GYWGDIVSSPYLSFGIETENKELLKTQNNHYVKTAQDISEVNVLELFECLAARGRSPLNDDPPNTSS---------SCCQSTESLKTEENSQSDPSASQTQPVEHSPTQELDLLNVNGVKVSFLSPDS-LSKLPLKSKYRNLFNTIFCSASMVHQLDSA--LREIAAPDAALVIELATFLLDLSKEQVSGFAVRVKEIAEKSGFT--PVH---NQNSDKYAVFT 465          
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Zebrafish
Match: dnaaf3 (dynein, axonemal, assembly factor 3 [Source:NCBI gene;Acc:100148431])

HSP 1 Score: 272.322 bits (695), Expect = 1.974e-85
Identity = 158/457 (34.57%), Postives = 264/457 (57.77%), Query Frame = 1
Query:   37 ESLGVINWWGFTPALDLFDNELLEIDECTDKADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAK 1407
            E  G + WWGF PA DL +++  ++     + ++N+LLVGSGD RHIL++++ L      H+  ++IEN +E+ ARQ+L L I+L+P ++M +  KTE+FLE++GN  +R+++ E  ++AA +L   +T S    +     +    LKFKERD L  IFKLW+   S    +S  WD R+RQ+LG+RYD+    FDWD ++ LH RG  +IN  +Y+ WR  GV F++R+  Y  +N++L S RV    G    +R GYWGDIV SP++ FGI + N  L +  NN ++  A  +SE N+ E+F  +A   +     +P  T++            +  E  + ++ +Q    A +   +       LDL+ V  VK+ FL  +S L  +  K+K+++ F+ ++ SAS++ QL++   L  I  P   L++E+  ++++++ +Q+  F  +V E+A+
Sbjct:    8 EGAGCVTWWGFGPARDLLNSDTHKVRL---QEELNVLLVGSGDPRHILKTITGLTHSDTLHV--WVIENSMEVIARQLLLLYISLLPPDKMSVHKKTEVFLEVFGNLEIRKETEESVKKAAAQLSISITYSLSSDSLSHSCLDTSLLKFKERDELVRIFKLWERPPSAPASVSKVWDARVRQHLGSRYDSRQGAFDWDLNMKLHQRGCGVINKHQYAKWRETGVTFEMREGLYQTANQSLLSTRVFNHRGNGVALR-GYWGDIVSSPYLSFGIETENKELLKTQNNHYVKTAQDISEVNLLELFECLAARGRSPLNEDPPNTSS---------SCCQSTESRKTEENSQSDPSASQTQPVEHSPTQELDLLNVNGVKVSFLSPDS-LSKLPLKSKYRNLFNTIFCSASMVHQLDSA--LREIAAPDAALVIELATFLLDLSKEQVSGFAVKVKEIAE 446          
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Xenopus
Match: ENSXETT00000037166.1 (dynein, axonemal, assembly factor 3 [Source:NCBI gene;Acc:100493459])

HSP 1 Score: 281.567 bits (719), Expect = 1.174e-88
Identity = 163/456 (35.75%), Postives = 262/456 (57.46%), Query Frame = 1
Query:   58 WWGFTPALDLFDNELLEIDE---CTDK-ADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEAC--PPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAK 1407
            WWG TPALDL  + L E  E   C+D   ++NILLVG GD RHIL+++    +     + F+IIE+ LEL AR ML L +AL    +MGLQ+K+ELFLEL+GN L+R ++  + ++ +   I++VT+ P +  +   L+++ ++KFKERD LE IFK W++ + K F +   WD + RQNLG RYD+    +DWD S+ LHDRGA +INSREY++WR KGVAF  R+  YD+ N+TLAS  V+  + G K +  GYWGDI  SP+I FGI +   +L Q +N   +  A  ++++N+  +FH++A  + Y   ++  A + + + D + +  +EQ   E  +  A+                    LI + NV++ FLP++ +        +    F+  +   SL   +     P    I   +  L++E+ K++V++  +++  +   V ++A+
Sbjct:   12 WWGLTPALDLQMHCLPEATENLSCSDDLPELNILLVGGGDGRHILKTICQASRWPHRKLKFFIIESDLELLARHMLFLSLALEHPEQMGLQEKSELFLELFGNSLIRNKTASYLQEKSELFIRYVTD-PDYQQSNVPLLNLSSIKFKERDKLEDIFKFWRNADPKLFPIDKYWDEKNRQNLGRRYDSRKGAYDWDLSMKLHDRGAGVINSREYNYWREKGVAFMNREGVYDIPNKTLASQMVVPQSSG-KVLARGYWGDITASPYIAFGIETEEESLLQTANGVHVKSAQDIAQHNMISLFHELAYGKIYSVPASGQAESELAKTDSDYKTNEEQTVVETTEREAE---------DGDTNQSKCNRLITLNNVEIHFLPLSWV-------NELHCRFNQQFLQPSLFLVMVHFLKPEYKFIAASKATLVLELTKFMVDLQTEKLQDYVTIVAKLAQ 449          
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Xenopus
Match: ENSXETT00000024360.1 (dynein, axonemal, assembly factor 3 [Source:NCBI gene;Acc:100493459])

HSP 1 Score: 281.182 bits (718), Expect = 1.890e-88
Identity = 141/326 (43.25%), Postives = 210/326 (64.42%), Query Frame = 1
Query:   58 WWGFTPALDLFDNELLEIDE---CTDK-ADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVD 1023
            WWG TPALDL  + L E  E   C+D   ++NILLVG GD RHIL+++    +     + F+IIE+ LEL AR ML L +AL    +MGLQ+K+ELFLEL+GN L+R ++  + ++ +   I++VT+ P +  +   L+++ ++KFKERD LE IFK W++ + K F +   WD + RQNLG RYD+    +DWD S+ LHDRGA +INSREY++WR KGVAF  R+  YD+ N+TLAS  V+  + G K +  GYWGDI  SP+I FGI +   +L Q +N   +  A  ++++N+  +FH++A  + Y   ++  A + + + D
Sbjct:   23 WWGLTPALDLQMHCLPEATENLSCSDDLPELNILLVGGGDGRHILKTICQASRWPHRKLKFFIIESDLELLARHMLFLSLALEHPEQMGLQEKSELFLELFGNSLIRNKTASYLQEKSELFIRYVTD-PDYQQSNVPLLNLSSIKFKERDKLEDIFKFWRNADPKLFPIDKYWDEKNRQNLGRRYDSRKGAYDWDLSMKLHDRGAGVINSREYNYWREKGVAFMNREGVYDIPNKTLASQMVVPQSSG-KVLARGYWGDITASPYIAFGIETEEESLLQTANGVHVKSAQDIAQHNMISLFHELAYGKIYSVPASGQAESELAKTD 346          
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Mouse
Match: Dnaaf3 (dynein, axonemal assembly factor 3 [Source:MGI Symbol;Acc:MGI:3588207])

HSP 1 Score: 267.314 bits (682), Expect = 2.871e-82
Identity = 164/464 (35.34%), Postives = 243/464 (52.37%), Query Frame = 1
Query:   46 GVINWWGFTPALDLFDNELLEIDECTDK------ADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWK--DENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRD-ANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSA-TATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAK 1407
            G ++WWG +PALDL   E   +D  +         +++ LL+GS D RH+L++L+          NFY++EN LE  AR ML   +AL    +MGLQ+++E FLEL+GN L+R     F R  A  L   V         L  L  +  LKF+ERD LEA+F+ W   ++  + F +S  WD RLR  LG+RYDA   V DWD  + LHDRGA +I+ +E+  WR  GVAF+LRD + Y V NRTLASGR+L +  G++    GYWGDI   PF+ FGI + + +L +  N Q +  AS ++++N++E+F ++A  R       P A    + E +  +            D PAQ                            + FLP++S    +  KT +Q  F L+Y S  ++  L   P L   + P G L+VE+ +Y+V++   ++  F  RV E+A+
Sbjct:   12 GSVSWWGLSPALDL-QAESPPVDPDSQSKTERKVPELDALLLGSVDGRHMLRTLARAALWPLRSFNFYVLENNLEAVARHMLIFSLALEEPEKMGLQERSETFLELWGNALLRPSVAAFLRAQASHLANLVLEPDRLEEQLPWL-SLRLLKFRERDALEAVFRFWSGGEKGPEVFPMSRLWDSRLRHYLGSRYDARRGVADWDLRMKLHDRGAQVIHIQEFRRWRDTGVAFELRDLSAYHVPNRTLASGRLL-SHRGERVAARGYWGDIATGPFMAFGIEADDQSLLRTRNGQPVKTASEITQHNVTELFREVAAWR------GPRAIQGNVEETESPEP-----------DVPAQE------------------------PFTIHFLPLDS-SQTLHHKTCYQGRFQLLYVSCGMVHLL--SPELGACVAPGGNLVVELARYLVDLRPKELKAFSDRVVEIAR 428          
BLAST of dynein assembly factor 3, axonemal vs. UniProt/SwissProt
Match: sp|Q32NQ7|DAAF3_XENLA (Dynein assembly factor 3, axonemal OS=Xenopus laevis OX=8355 GN=dnaaf3 PE=2 SV=1)

HSP 1 Score: 295.819 bits (756), Expect = 1.552e-93
Identity = 163/456 (35.75%), Postives = 270/456 (59.21%), Query Frame = 1
Query:   55 NWWGFTPALDL----FDNELLEIDECTDKADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKA 1410
            +WWGF+PALDL        +  +       ++NILLVG GD RH+L+++    +     + F+IIE+ LEL AR ML L +AL    +MGLQ+K+ELFLEL+GN L+R ++  + ++ +   I+ VT+ P +   +   +++ ++KFKERD LE IFK W+  + K F +   WD + R++LGTRYD+    +DWD S+ LHDRGA +INSREY++WR KGVAF  R+  YDV N+TLAS  V+  + G K +  GYWGDI  SP++ FGI +   +L + +N   +  A  ++ +NI  +FH++A  + Y   ++  A A + +   + +   +Q   E I++ A+         +           I + NV++ FLP+ S ++ +  K +F + F+L+YFS+S++  L+  P    I   +  L++E+ K++V++ AD++  + + V ++A+ 
Sbjct:   12 SWWGFSPALDLQVHCLQGTIQHLTSSDGLPELNILLVGGGDGRHLLKTICQASRWPHRKLKFFIIESDLELLARHMLFLSLALEHPEQMGLQEKSELFLELFGNSLIRSKTANYLQEKSELFIQCVTD-PDYQQCVMPFLNLSSIKFKERDELEDIFKFWRIADPKLFPIDKYWDGKNRRHLGTRYDSRKGAYDWDLSMKLHDRGAGVINSREYNYWREKGVAFMNREGVYDVPNKTLASQMVVPQSSG-KVLTRGYWGDITTSPYVAFGIETEEKSLLETANGVHVKSAQEIALHNIISLFHELATGKVYSVPASGQAEAELVKPVGDYKTNGDQTAAETIEEKAE---------AEDTKQSKYKGFITLNNVEIHFLPL-SWVNELHCKGRFTNFFNLLYFSSSMVHLLK--PEYKVIAASKATLVLELTKFMVDLQADKVQSYVSIVTKLAQG 453          
BLAST of dynein assembly factor 3, axonemal vs. UniProt/SwissProt
Match: sp|F6S9E6|DAAF3_XENTR (Dynein assembly factor 3, axonemal OS=Xenopus tropicalis OX=8364 GN=dnaaf3 PE=3 SV=2)

HSP 1 Score: 283.493 bits (724), Expect = 3.867e-89
Identity = 164/456 (35.96%), Postives = 260/456 (57.02%), Query Frame = 1
Query:   58 WWGFTPALDL----FDNELLEID-ECTDK-ADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAK 1407
            WWG TPALDL          EI   C+D   ++NILLVG GD RHIL+++    +     + F+IIE+ LEL AR ML L +AL    +MGLQ+K+ELFLEL+GN L+R ++  + ++ +   I++VT+ P +  +   L+++ ++KFKERD LE IFK W++ + K F +   WD + RQNLG RYD+    +DWD S+ LHDRGA +INSREY++WR KGVAF  R+  YD+ N+TLAS  V+  + G K +  GYWGDI  SP+I FGI +   +L Q +N   +  A  ++++N+  +FH++A  + Y   ++  A + + + D                  + Y     +           + LI + NV++ FLP            +F + F+L+YFS S++  L+  P    I   +  L++E+ K++V++  +++  +   V ++A+
Sbjct:   12 WWGLTPALDLQMHLHPPPPPEIYCSCSDDLPELNILLVGGGDGRHILKTICQASRWPHRKLKFFIIESDLELLARHMLFLSLALEHPEQMGLQEKSELFLELFGNSLIRNKTASYLQEKSELFIRYVTD-PDYQQSNVPLLNLSSIKFKERDKLEDIFKFWRNADPKLFPIDKYWDEKNRQNLGRRYDSRKGAYDWDLSMKLHDRGAGVINSREYNYWREKGVAFMNREGVYDIPNKTLASQMVVPQSSG-KVLARGYWGDITASPYIAFGIETEEESLLQTANGVHVKSAQDIAQHNMISLFHELAYGKIYSVPASGQAESELAKTD------------------SDYKTNEEQ----------TVGLITLNNVEIHFLP------------RFNNFFNLLYFSCSMVHFLK--PEYKFIAASKATLVLELTKFMVDLQTEKLQDYVTIVAKLAQ 423          
BLAST of dynein assembly factor 3, axonemal vs. UniProt/SwissProt
Match: sp|F1MLB4|DAAF3_BOVIN (Dynein assembly factor 3, axonemal OS=Bos taurus OX=9913 GN=DNAAF3 PE=3 SV=2)

HSP 1 Score: 280.796 bits (717), Expect = 5.898e-87
Identity = 166/474 (35.02%), Postives = 260/474 (54.85%), Query Frame = 1
Query:   31 SKESLGVINWWGFTPALDLFDNELLEIDECT------DKADINILLVGSGDSRHILQSLS--SLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQ---LIHIDNLKFKERDILEAIFKLWK--DENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDAN-YDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKA 1410
            S    G ++WWG +PA+DL   E   +D  +      +  ++N+LL+GS D RH+L++L+  +L+ R++ H  FY++EN LE  AR +L   +AL    +MGLQ+++E FLE++GN L+R     F R  A +L   V      P  L++    + +  LKF+ERD LEA+F+ W   ++  ++F +S  WD RLRQ LG+RYDA   V DWD  + LHDRGA +I++RE+  WR  GVAF+LRD++ Y V NRTLASGR+L +  G++    GYWGDI   PF+ FGI + +  L + SN Q +  A  ++E+N++E+F ++A         +P A                        DP Q   GA         G       A     + FL ++S    +  K+ +   F L+Y +  ++  L A   L   + P G+L+VE+ +++V++  +Q+  F +RV E+A+A
Sbjct:    7 SGTGFGSVSWWGLSPAVDL-QAESPPVDPDSQAETEHETPELNVLLLGSVDGRHLLRTLARAALWPRRRFH--FYVLENNLEAVARHILIFSLALEDPEKMGLQERSETFLEVWGNALLRPPVAAFVRAQAGRLAHLVPE----PDRLAEQLPWLSLGALKFRERDALEAVFRFWAGGEKGPEAFPMSRLWDSRLRQYLGSRYDARHGVSDWDLHMKLHDRGARVIHTREFRRWRDTGVAFELRDSSAYHVPNRTLASGRLL-SHRGERVAARGYWGDIATGPFVAFGIEADDETLLRTSNGQPVKTACEITEHNVAELFREMAA------WGHPRAAE---------------------GDPEQVQGGA--------EGSSEPAAPAPEPFTVHFLSLDS-AHTLHHKSCYMGQFQLLYVACGMVHLLSAE--LGACVAPGGRLIVELAQFLVDLRQEQLQAFSSRVGELAQA 434          
BLAST of dynein assembly factor 3, axonemal vs. UniProt/SwissProt
Match: sp|F1Q7Z7|DAAF3_DANRE (Dynein assembly factor 3, axonemal OS=Danio rerio OX=7955 GN=dnaaf3 PE=2 SV=2)

HSP 1 Score: 272.322 bits (695), Expect = 1.490e-84
Identity = 158/457 (34.57%), Postives = 264/457 (57.77%), Query Frame = 1
Query:   37 ESLGVINWWGFTPALDLFDNELLEIDECTDKADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAK 1407
            E  G + WWGF PA DL +++  ++     + ++N+LLVGSGD RHIL++++ L      H+  ++IEN +E+ ARQ+L L I+L+P ++M +  KTE+FLE++GN  +R+++ E  ++AA +L   +T S    +     +    LKFKERD L  IFKLW+   S    +S  WD R+RQ+LG+RYD+    FDWD ++ LH RG  +IN  +Y+ WR  GV F++R+  Y  +N++L S RV    G    +R GYWGDIV SP++ FGI + N  L +  NN ++  A  +SE N+ E+F  +A   +     +P  T++            +  E  + ++ +Q    A +   +       LDL+ V  VK+ FL  +S L  +  K+K+++ F+ ++ SAS++ QL++   L  I  P   L++E+  ++++++ +Q+  F  +V E+A+
Sbjct:    8 EGAGCVTWWGFGPARDLLNSDTHKVRL---QEELNVLLVGSGDPRHILKTITGLTHSDTLHV--WVIENSMEVIARQLLLLYISLLPPDKMSVHKKTEVFLEVFGNLEIRKETEESVKKAAAQLSISITYSLSSDSLSHSCLDTSLLKFKERDELVRIFKLWERPPSAPASVSKVWDARVRQHLGSRYDSRQGAFDWDLNMKLHQRGCGVINKHQYAKWRETGVTFEMREGLYQTANQSLLSTRVFNHRGNGVALR-GYWGDIVSSPYLSFGIETENKELLKTQNNHYVKTAQDISEVNLLELFECLAARGRSPLNEDPPNTSS---------SCCQSTESRKTEENSQSDPSASQTQPVEHSPTQELDLLNVNGVKVSFLSPDS-LSKLPLKSKYRNLFNTIFCSASMVHQLDSA--LREIAAPDAALVIELATFLLDLSKEQVSGFAVKVKEIAE 446          
BLAST of dynein assembly factor 3, axonemal vs. UniProt/SwissProt
Match: sp|Q95K25|DAAF3_MACFA (Dynein assembly factor 3, axonemal OS=Macaca fascicularis OX=9541 GN=DNAAF3 PE=2 SV=1)

HSP 1 Score: 272.707 bits (696), Expect = 7.298e-84
Identity = 160/471 (33.97%), Postives = 251/471 (53.29%), Query Frame = 1
Query:   31 SKESLGVINWWGFTPALDL-FDNELLEIDECTDK----ADINILLVGSGDSRHILQSLS--SLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWK--DENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDAN-YDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVP-NVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKA 1410
            S    G ++WWG +PALDL  ++  ++ D   D      ++++LL+GS D RH+L++LS   L+ R++   NF+ +EN LE  AR ML   +AL    +MGLQ+++E FLE++GN L+R     F R  A  L   V         L  L  +  LKF+ERD LEA+F+ W   ++  ++F +S  WD RLR  LG+RYDA   V DWD  + LHDRGA +I+++E+  WR  GVAF+LRD++ Y V NRTLASGR+L +  G++    GYWGDI   PF+ FGI + + +L + SN Q +  A  ++++N++E+  ++A   +                                   A+   G P                + P +  + FLP+NS  + +  K+ +   F L+Y +  ++  L   P L   + P G L+VE+ +Y+V++  +Q+  F  +V E A+A
Sbjct:    7 SGRGFGSVSWWGLSPALDLQAESPPVDPDSQADTVHSTPELDVLLLGSVDGRHLLRTLSRAKLWPRRR--FNFFALENNLEAVARHMLIFSLALEDPEKMGLQERSETFLEVWGNALLRPPVAAFVRAQADLLAHLVPEPDRLEEQLPWL-SLRALKFRERDALEAVFRFWAGGEKGPEAFPMSRLWDSRLRHYLGSRYDARHGVSDWDLRMKLHDRGAQVIHTQEFRRWRDTGVAFELRDSSAYHVPNRTLASGRLL-SYRGERVAARGYWGDIATGPFVAFGIEADDESLLRTSNGQPVKTAGEITQHNVTELLREVAAWGR-----------------------------------ARASRGHPEEQQRSEASPEPGTPASTPESFTVHFLPLNS-AETLHHKSCYNGRFQLLYVACGVVHLL--SPELGACVAPGGNLIVELARYLVDMRQEQLQGFNTQVGERAQA 435          
BLAST of dynein assembly factor 3, axonemal vs. TrEMBL
Match: A0A1S3IEE1 (dynein assembly factor 3, axonemal OS=Lingula unguis OX=7574 GN=LOC106163551 PE=4 SV=1)

HSP 1 Score: 347.436 bits (890), Expect = 2.251e-110
Identity = 196/483 (40.58%), Postives = 289/483 (59.83%), Query Frame = 1
Query:   37 ESLGVINWWGFTPALDLFDNELLEIDECTDKAD-----INILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXI--------DDPAQYLNGAPRXXXXXXXXXXXXXXXAVP--NVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKAHSMQ-AFPVN 1437
            +  G I WWGF+P LDL D +L    +     D     +NIL+VG+GD RHIL++L+  Y+  +  INFYI+E  +E YAR +L L IAL   NRMGLQ+KTELF+EL+GN LVR+Q+ E+    + + IK VT+       +  +I + +LKFKERD LE IFK W++++ + FD+S CWDLRLRQ LGTRYDA   VFDWD ++ L +RGA II+  EY+ WR+ GVAF++R+ NY+  N+TLASG +L+   GDK  R GYWGD++  PFI FG+ S   + F+  N      A  V+E+NI+ + H+I    KY     P    T  E +++  + QE +E++++        ++  Q     P   +      L  + ++VP  N K+ FLP+N   D ++KK+K+Q  F++ YFS S++  L   P LA  L  +  +++E+  Y++ +  + I  F  +V  MAKA   + A P N
Sbjct:   43 DGFGSITWWGFSPVLDLQDKDLCSHLQNVQLGDGELPELNILMVGAGDCRHILKTLAHTYRWPRQKINFYILEANMECYARHLLLLTIALESQNRMGLQEKTELFMELFGNTLVRQQTCEYLESMSNEFIKMVTDFDHLEKKMP-IIDMSSLKFKERDFLEGIFKFWRNKDHQIFDVSKCWDLRLRQYLGTRYDARNGVFDWDYNMKLVERGAKIIHKSEYNRWRNTGVAFEIREGNYEFPNKTLASGLLLKTQDGDKVARRGYWGDVLVGPFIAFGLESEEKSFFKTYNGLHTKMAGDVTEFNITSMLHEILNKTKY---ELPQMEDTKEEEEQKGPKLQEIREEDELEEEEEEDQNNVKQPEPEPPAADAETEQTPLEKEFVSVPVENFKVHFLPLNCAQD-LSKKSKYQKLFNIAYFSNSMVHHL--TPELAATLADKASVILELTLYMLELRPENIKLFSEKVTNMAKAAGCEVAAPCN 518          
BLAST of dynein assembly factor 3, axonemal vs. TrEMBL
Match: V4BGU5 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_227827 PE=4 SV=1)

HSP 1 Score: 342.813 bits (878), Expect = 1.959e-109
Identity = 185/470 (39.36%), Postives = 278/470 (59.15%), Query Frame = 1
Query:   37 ESLGVINWWGFTPALDLFDNEL------LEIDECTDKADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYV----ETSNPSATATITEVDXXXXXXXXXXXXXXIDDPA--QYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKA 1410
            +  G I WWGF+PALDL +N +      L+I   + + ++NILLVG+GD RHIL +++  ++     ++FY++E  LELYAR ML L IAL P NRMGLQ+KTELFLE+Y N  +R+Q+ E  +  + + IK VT+       L  +  +  LKFKERD LE IFK W++ +   FD+  CWDLR R+ LG RYD+  NV+DWD  + L D GA II+  EY++WR  G+AF +RD  YD+ N+TLASG V     G+K  R GYWGD+V  PF+ FGI S   + F+++N+Q+      V EYN+  + +++    +Y+    ET   SA  T    +EE+ E++        +  A  Q+    P                 V NVK+ FLP+NS +D V KK+K+Q  F ++YFS S++  L+  P +  +L+    L++E  K+++ +  +QI+ + +++ EMA+ 
Sbjct:    3 DGFGSITWWGFSPALDLQENGIDEMLKKLKIKHDSGEEELNILLVGAGDIRHILTTIARSWRHSPHKLHFYVLETSLELYARDMLLLNIALEPQNRMGLQEKTELFLEVYSNTFIRQQTCENIQSLSTEFIKMVTDFDYLEKKLP-VFDLSQLKFKERDFLEGIFKFWRNPDKAVFDVEKCWDLRERKRLGVRYDSKKNVYDWDYHMKLIDTGADIIHKVEYNNWRESGIAFTIRDGTYDIPNKTLASGMVF-TQEGEKHARRGYWGDMVVGPFLTFGIESEEKSFFKKNNDQYTKTCENVCEYNVMSLLYELINKDRYILPKPETQPESAKLTEITEEEEEAEEEVTSSVTNGNQEADNQHKEMIP-----------------VDNVKITFLPINS-IDDVKKKSKYQKLFHMLYFSNSMVHHLK--PDITSVLKDDASLILESAKFMIELKPEQIEEYGSKITEMAET 450          
BLAST of dynein assembly factor 3, axonemal vs. TrEMBL
Match: A0A3M6TVC4 (Uncharacterized protein OS=Pocillopora damicornis OX=46731 GN=pdam_00000727 PE=4 SV=1)

HSP 1 Score: 334.339 bits (856), Expect = 4.492e-106
Identity = 188/502 (37.45%), Postives = 287/502 (57.17%), Query Frame = 1
Query:   37 ESLGVINWWGFTPALDLFDN-----------ELLEIDECTDKADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLR-DANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYV------ETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKAHSMQAFPVNCAAGLDKSEYVVYTLNQ 1488
            +  G I WWGF+PALDL D             + E +    +   NILL+G+GD RHIL++++  Y+ ++  +   ++EN LE+YAR +L L IAL P  R+GLQ+KTELFLEL+GN LVR Q+ E+ +Q + + I+ VT+S      L  + +   LKFKERD LE+IFK W++ + K FD+   WD RLRQ L  RYD+  N++DWD S+ L  + A+IIN REY  WR +GVAF+LR D+ Y+V NRTLASG +++   G++  R GYWGDI+ SP++ FG  S    LFQ SN Q +  A+ VSEYNI+ + H+I    KY+      E       AT+ E+ EE+ E  +    +  D P +                       V   ++ FLP+N + D ++KK+K+   FD++YFS S++  L   P +  I   +  ++ E  K+++++  +Q+  +  ++  MA +   +A  V+ A G +K  +  +T  +
Sbjct:    3 DGFGAITWWGFSPALDLQDRYQSLASKIKNLNISESNGANKRGKCNILLIGAGDCRHILKTIAHTYRHERRELCICVVENNLEIYARHLLLLSIALEPKERLGLQEKTELFLELFGNSLVRPQASEYLQQKSSEFIRMVTDSDYLEAHLP-VFNFSQLKFKERDQLESIFKFWRNSDPKIFDICRHWDERLRQYLAVRYDSRENIYDWDYSMKL-SKKASIINRREYKSWREQGVAFELRSDSTYEVINRTLASGIIIK-KDGERVARRGYWGDIINSPYLAFGTESEEEFLFQTSNGQHVRTATNVSEYNITALMHEILTGEKYIPPKIEDEKERKKEMATLQEIGEEEIESDQMGNTKDGDTPKE----------------------TVHATQVTFLPLNCVPD-LSKKSKYNKLFDVIYFSNSMVHLL--TPEVKEIFSDEAVIIAETTKFMLDLKPEQMQEYMKKITSMAVSAECKA--VDHADG-EKDAHAFFTFQR 473          
BLAST of dynein assembly factor 3, axonemal vs. TrEMBL
Match: V9KLX2 (Uncharacterized protein OS=Callorhinchus milii OX=7868 GN=dnaaf3 PE=2 SV=1)

HSP 1 Score: 328.176 bits (840), Expect = 3.179e-103
Identity = 184/495 (37.17%), Postives = 278/495 (56.16%), Query Frame = 1
Query:   37 ESLGVINWWGFTPALDL------FDNELLEIDECTDKADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAV-------------------------PNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKAHSMQAF 1428
            +  G + WWGF+PA+D          E L + E     ++NIL+VG+GDSRH+L +L   ++  +  INFYI+EN LEL  R +L L IAL    +MGLQ+K+EL LEL+GN L+R Q+  + ++ A   I++VT+ P +      L+ +  LKFKERD+LE  FK W++ + K F ++  WDLR R+ LG RYD+   V+DWD  + LH+RGA +IN++EY  WR +G AF++R+  YDV N+TLASG +L    G+K    G+WGDIV SPFI FGI +   +L + +N   +  A  +S YNI+ +FH++    KY     PS  AT+ E+      ++E  + E  +DP    N +          +       V                         P+VK+ FLP+N  + ++ KK+K+ + F+++YFS S++ QL+  P L     PQ  LLVEM K++++I  D   +F +R  E+A+      F
Sbjct:    8 DGFGSVTWWGFSPAMDFQKESPSVSTEALGLQEG-GLPELNILMVGAGDSRHLLTTLCRAHRWAQRKINFYIVENNLELIGRHLLFLSIALERSEQMGLQEKSELLLELFGNSLIRSQTAAYLQEKANLFIRYVTD-PDYLQEKMPLVDLSALKFKERDLLEGTFKFWRNPDQKVFQINKLWDLRNRKYLGIRYDSRKGVYDWDLHMKLHERGAVVINNQEYFRWREQGFAFEVREGVYDVPNKTLASG-LLVNHKGEKVAARGFWGDIVTSPFIAFGIETEEKSLLKTANGIHVKTAQNISHYNITALFHELVTREKYSPMEQPS-QATLCEI-----FEEEPTKVETANDPYNTKNKSTDNPEEQHDVMKEKVQAEVQENKLMGDKMSEGSHSASEYDYLPLPHVKIHFLPLNC-VPHLHKKSKYTNLFNVIYFSCSMLDQLK--PELKQTAAPQATLLVEMAKFLLDIQKDLTAKFASRATEIAEEAGFVPF 490          
BLAST of dynein assembly factor 3, axonemal vs. TrEMBL
Match: A0A401SV25 (Uncharacterized protein OS=Chiloscyllium punctatum OX=137246 GN=chiPu_0012669 PE=4 SV=1)

HSP 1 Score: 324.709 bits (831), Expect = 5.137e-102
Identity = 183/487 (37.58%), Postives = 287/487 (58.93%), Query Frame = 1
Query:   31 SKESLGVINWWGFTPALDLFDNELL-EIDECTDKAD----INILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXX----------------XXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKAHSMQAF 1428
            S E  G + WWGF+PA+D  +  L   ++  + K D    +NIL+VG+GDSRH+L ++S  +   +  INFYI+EN LEL  R +L L IAL P  +MGL++K EL LEL+GN L+R Q+  + +  A   I ++T+       L  +I +  LKFKERD LEAIFK W++ + K F ++  WD+R RQ LG RYD+   V+DWD  + L++RGA IIN+R+Y  WR KG+AF++R+  YDV N++LASG +L    G+K    GYWGDIV SP+I FGI +   +L + +N   +  A  +S+YNI+ + H++    KY   + P+  AT+ E+  E++E   + +   I + + + +  P I++                         D + +P+VK+ FLP+N + + + KK+ +   F+++YFS S++ QL+  P L  I   +  L+VE+PK+I+++  + + +F +R  EMAK      F
Sbjct:    6 SGEGFGAVAWWGFSPAMDFQEKGLTASLEALSSKEDSVPELNILMVGAGDSRHLLTTISRNHHWPRRKINFYIVENNLELLGRHLLFLTIALEPPEQMGLREKCELLLELFGNSLIRSQTATYLQDKANLFIHYITDLDFLHKKLP-MIDMSGLKFKERDQLEAIFKFWRNPDQKIFQINRLWDMRNRQYLGNRYDSRIGVYDWDLHMKLYERGARIINNRQYYQWRDKGLAFEVREGLYDVPNKSLASG-LLMNYKGEKVPARGYWGDIVTSPYIAFGIETEEQSLLKTANGVHVKTAQDISQYNITALLHELVAKEKY---ALPTEKATLHEI-TEEEEGGMENDSSNIKNESTHTHQHPEIVTEEGTMKQSNDLDDTNSSGGHCAAEYDSLPLPDVKIHFLPMNCVTE-LHKKSNYCERFNVIYFSCSMLHQLK--PELKQIAALRATLIVELPKFILDLRKEVMTKFASRTIEMAKEAGFVPF 483          
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Cavefish
Match: dnaaf3l (dynein axonemal assembly factor 3 [Source:NCBI gene;Acc:103035356])

HSP 1 Score: 259.225 bits (661), Expect = 3.253e-80
Identity = 161/501 (32.14%), Postives = 265/501 (52.89%), Query Frame = 1
Query:   37 ESLGVINWWGFTPALDLFDNELLEIDECTDKADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIAC------------NRKYVETSNPSATATITEVDXX-----XXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKAHSMQAFPVNCAAGLDKSEYVVYTLNQ 1488
            E  G I WWGF PA DL +   L         ++N+LL+G+ D +HIL++++ L +    H+  ++IEN +E+ ARQ+L L ++L     MG  +KTE+FLEL+GN  +R Q+ E  +  A +L   VT++    T      +   LKFKERD L  +FK W+   S    +   WD R+RQ+LGTRYD+    FDWD ++ LH +G  +IN ++Y  WR +GVAF++R+  Y  +N++L S RV    G    VR GYWGD+V SP++ FGI + N  L +  N  ++  A  +S  N+  +F  ++             N    ET      +T  E ++        E Q  +++ Q+ D  + +              +  +L+++  V + FL  +S L  +  K+++   F+++Y SAS++ QL++   L GI  P   L+VE+ KY+++++ +Q   F  +V+++AK       P+   +G     Y V+TL +
Sbjct:    8 EGAGCITWWGFGPARDLLNAGTLR-----PAGEVNVLLLGAADPKHILKTIAGLKETDTLHV--WVIENSMEIVARQLLLLYLSLTTHEIMGRHEKTEVFLELFGNSEIRCQTEETLKHVAAQLSLAVTDTLDAHTQEHPCFNTSLLKFKERDELARLFKQWQHPPSSPASVFKAWDARVRQHLGTRYDSRKGCFDWDLTMKLHPKGCEVINRQQYIKWRERGVAFEMREGLYQTTNQSLISTRVFNHRGDRMAVR-GYWGDVVSSPYLSFGIETENKDLLKTQNKHYVKTAQDISIVNVQMLFESLSARGGHRGDSQNGSNSHATETQPQPGASTNDETEQHPQNQNSAENQTTKKETQVVDTERQIQTEVNTQPKTQHRTIQSELMSLGGVSVSFLSPDS-LSKLPVKSRYSHLFNVIYCSASMVHQLDSS--LKGIAAPDALLIVELAKYLLDLSKEQEAAFAVKVEQIAKEAGF--IPICNQSG---DAYAVFTLQK 492          
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Cavefish
Match: dnaaf3l (dynein axonemal assembly factor 3 [Source:NCBI gene;Acc:103035356])

HSP 1 Score: 236.498 bits (602), Expect = 4.876e-72
Identity = 147/484 (30.37%), Postives = 243/484 (50.21%), Query Frame = 1
Query:   37 ESLGVINWWGFTPALDLFDNELLEIDECTDKADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKAHSMQAFPVNCAAGLDKSEYVVYTLNQ 1488
            E  G I WWGF PA DL +   L         ++N+LL+G+ D +HIL++++ L +    H+        +  +   ML+               KTE+FLEL+GN  +R Q+ E  +  A +L   VT++    T      +   LKFKERD L  +FK W+   S    +   WD R+RQ+LGTRYD+    FDWD ++ LH +G  +IN ++Y  WR +GVAF++R+  Y  +N++L S RV    G    VR GYWGD+V SP++ FGI + N  L +  N  ++  A  +S  N+  +F  ++    +   S   + +  TE   +             D+  Q+     +  +          L+++  V + FL  +S L  +  K+++   F+++Y SAS++ QL++   L GI  P   L+VE+ KY+++++ +Q   F  +V+++AK       P+   +G     Y V+TL +
Sbjct:    8 EGAGCITWWGFGPARDLLNAGTLR-----PAGEVNVLLLGAADPKHILKTIAGLKETDTLHV--------VSFFFSCMLK---------------KTEVFLELFGNSEIRCQTEETLKHVAAQLSLAVTDTLDAHTQEHPCFNTSLLKFKERDELARLFKQWQHPPSSPASVFKAWDARVRQHLGTRYDSRKGCFDWDLTMKLHPKGCEVINRQQYIKWRERGVAFEMREGLYQTTNQSLISTRVFNHRGDRMAVR-GYWGDVVSSPYLSFGIETENKDLLKTQNKHYVKTAQDISIVNVQMLFESLSARGGHRGDSQNGSNSHATETQPQPGASTN-------DETEQHPQN--QNSAENQTTKKETQLMSLGGVSVSFLSPDS-LSKLPVKSRYSHLFNVIYCSASMVHQLDSS--LKGIAAPDALLIVELAKYLLDLSKEQEAAFAVKVEQIAKEAGF--IPICNQSG---DAYAVFTLQK 445          
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Nematostella
Match: EDO37526 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFN1])

HSP 1 Score: 150.214 bits (378), Expect = 6.472e-43
Identity = 80/184 (43.48%), Postives = 116/184 (63.04%), Query Frame = 1
Query:   37 ESLGVINWWGFTPALDL--------FDNELLEIDECTDKADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCW 564
            ++ G I WWGF+PALDL        F+N  L++     +  +N+L++G+GD RHIL++LS   +  K  IN Y++EN LE+ AR +L L IAL P   +GLQ+KTELFLEL+GN L+R Q+ E+ ++ +   I+ VT+       L  +  I  LKFKERD+LE IFK W++ + K FD+   W
Sbjct:    3 DAFGAITWWGFSPALDLQEICLSSKFEN--LKLSGEGQEKPLNVLMIGAGDCRHILKTLSHAKRWPKRKINIYVVENNLEVLARHLLLLSIALEPQVSLGLQEKTELFLELFGNSLIRPQASEYLQRTSNDFIRMVTDFDYLEDKLP-IFDITQLKFKERDLLEGIFKFWRNSDPKFFDICKFW 183          
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Medaka
Match: dnaaf3l (dynein axonemal assembly factor 3 [Source:NCBI gene;Acc:101157011])

HSP 1 Score: 283.878 bits (725), Expect = 2.862e-90
Identity = 167/448 (37.28%), Postives = 247/448 (55.13%), Query Frame = 1
Query:   37 ESLGVINWWGFTPALDLFDNELLEIDECTDKADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKS----FDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQ 1368
            E  G I WWGF+PALDL     L +     + +I +LLVGSGD RHIL++++ L + K+ H+  Y+IE  +EL ARQ+L L IALIP   MG+ +KTE+FLE++GN  +R Q+ E  RQAA +L  FVT +    T+    ++   LKFKERD L  I K W   +S        +S  WD R+RQ+LGTRYD+    FDWD ++ LH +G  +IN ++Y  WR +G+AF++R+  Y + N TL S RVL +  GD     GYWGDIV SP++ FG+ + + +L +  N + +  A  +S  N+  +F  ++  R    TS P       E + E+   Q   +  +I D   +L G                      V L  LP+ S +  +  K K+ + F+ +YFS S + QL   P +  I  P   L+VE+ KYI++++ +Q
Sbjct:    8 EGAGCITWWGFSPALDL-----LSLGPEGSEEEIKVLLVGSGDPRHILKTIAGL-QEKRLHV--YVIETHMELVARQLLLLYIALIPQEIMGISEKTEVFLEVFGNSEIRSQTEEIVRQAASQLHLFVTET--IETSPHDCLNTTYLKFKERDELAKILKSWTRPSSPQHPAPISMSKAWDYRVRQHLGTRYDSPEGCFDWDLNMKLHKKGCGVINKQQYEQWRKRGLAFEMREGIYQIPNPTLLSSRVL-SRTGDSVAYRGYWGDIVSSPYLSFGVETDDKSLLKTQNGKHIKTAQDISFANVQGLFQTLSSRRSCPATSEP-------EKETEKPSMQPDLKSLRIKD-LMHLTG----------------------VSLTILPLES-VHKLPDKQKYSNFFNFMYFSVSCVHQL--GPMMGQIAAPDAVLVVELAKYILDLSKEQ 411          
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Medaka
Match: dnaaf3l (dynein axonemal assembly factor 3 [Source:NCBI gene;Acc:101157011])

HSP 1 Score: 283.878 bits (725), Expect = 2.862e-90
Identity = 167/448 (37.28%), Postives = 247/448 (55.13%), Query Frame = 1
Query:   37 ESLGVINWWGFTPALDLFDNELLEIDECTDKADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKS----FDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQ 1368
            E  G I WWGF+PALDL     L +     + +I +LLVGSGD RHIL++++ L + K+ H+  Y+IE  +EL ARQ+L L IALIP   MG+ +KTE+FLE++GN  +R Q+ E  RQAA +L  FVT +    T+    ++   LKFKERD L  I K W   +S        +S  WD R+RQ+LGTRYD+    FDWD ++ LH +G  +IN ++Y  WR +G+AF++R+  Y + N TL S RVL +  GD     GYWGDIV SP++ FG+ + + +L +  N + +  A  +S  N+  +F  ++  R    TS P       E + E+   Q   +  +I D   +L G                      V L  LP+ S +  +  K K+ + F+ +YFS S + QL   P +  I  P   L+VE+ KYI++++ +Q
Sbjct:    8 EGAGCITWWGFSPALDL-----LSLGPEGSEEEIKVLLVGSGDPRHILKTIAGL-QEKRLHV--YVIETHMELVARQLLLLYIALIPQEIMGISEKTEVFLEVFGNSEIRSQTEEIVRQAASQLHLFVTET--IETSPHDCLNTTYLKFKERDELAKILKSWTRPSSPQHPAPISMSKAWDYRVRQHLGTRYDSPEGCFDWDLNMKLHKKGCGVINKQQYEQWRKRGLAFEMREGIYQIPNPTLLSSRVL-SRTGDSVAYRGYWGDIVSSPYLSFGVETDDKSLLKTQNGKHIKTAQDISFANVQGLFQTLSSRRSCPATSEP-------EKETEKPSMQPDLKSLRIKD-LMHLTG----------------------VSLTILPLES-VHKLPDKQKYSNFFNFMYFSVSCVHQL--GPMMGQIAAPDAVLVVELAKYILDLSKEQ 411          
BLAST of dynein assembly factor 3, axonemal vs. Planmine SMEST
Match: SMESG000017259.1 (SMESG000017259.1)

HSP 1 Score: 902.508 bits (2331), Expect = 0.000e+0
Identity = 460/487 (94.46%), Postives = 464/487 (95.28%), Query Frame = 1
Query:   28 MSKESLGVINWWGFTPALDLFDNELLEIDECTDKADINILLVGSGDSRHILQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQAAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPSATATITEVDXXXXXXXXXXXXXXIDDPAQYLNGAPRXXXXXXXXXXXXXXXAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKAHSMQAFPVNCAAGLDKSEYVVYTLNQ 1488
            MSKESLGVINWWGFTPALDLFDNELLEIDECT  ADINILLVGSGDSRHILQSLSSLYKRKKC+INFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQA GGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNP ATATITEVD E+Q++QEQQEQEQIDDPAQYLN                 LIAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLI QLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKAHSMQAFPVNCAAGLDKSEYVVYTLNQ
Sbjct:    1 MSKESLGVINWWGFTPALDLFDNELLEIDECT--ADINILLVGSGDSRHILQSLSSLYKRKKCNINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKTELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNLKFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDWDCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQATGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSEYNISEIFHQIACNRKYVETSNPLATATITEVD-EEQQEQEQQEQEQIDDPAQYLND----------------LIAVPNVKLIFLPVNSLLDYVAKKTKFQSNFDLVYFSASLIGQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDRFRARVDEMAKAHSMQAFPVNCAAGLDKSEYVVYTLNQ 468          
The following BLAST results are available for this feature:
BLAST of dynein assembly factor 3, axonemal vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
DNAAF32.191e-8434.86dynein axonemal assembly factor 3 [Source:HGNC Sym... [more]
DNAAF37.298e-8434.86dynein axonemal assembly factor 3 [Source:HGNC Sym... [more]
DNAAF38.948e-8133.33dynein axonemal assembly factor 3 [Source:HGNC Sym... [more]
DNAAF37.749e-7234.84dynein axonemal assembly factor 3 [Source:HGNC Sym... [more]
DNAAF35.432e-2137.98dynein axonemal assembly factor 3 [Source:HGNC Sym... [more]
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BLAST of dynein assembly factor 3, axonemal vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of dynein assembly factor 3, axonemal vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG176695.127e-5133.03gene:FBgn0034352 transcript:FBtr0086722[more]
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BLAST of dynein assembly factor 3, axonemal vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
dnaaf3l7.013e-8934.72dynein, axonemal, assembly factor 3 like [Source:Z... [more]
dnaaf31.974e-8534.57dynein, axonemal, assembly factor 3 [Source:NCBI g... [more]
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BLAST of dynein assembly factor 3, axonemal vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSXETT00000037166.11.174e-8835.75dynein, axonemal, assembly factor 3 [Source:NCBI g... [more]
ENSXETT00000024360.11.890e-8843.25dynein, axonemal, assembly factor 3 [Source:NCBI g... [more]
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BLAST of dynein assembly factor 3, axonemal vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Dnaaf32.871e-8235.34dynein, axonemal assembly factor 3 [Source:MGI Sym... [more]
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BLAST of dynein assembly factor 3, axonemal vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q32NQ7|DAAF3_XENLA1.552e-9335.75Dynein assembly factor 3, axonemal OS=Xenopus laev... [more]
sp|F6S9E6|DAAF3_XENTR3.867e-8935.96Dynein assembly factor 3, axonemal OS=Xenopus trop... [more]
sp|F1MLB4|DAAF3_BOVIN5.898e-8735.02Dynein assembly factor 3, axonemal OS=Bos taurus O... [more]
sp|F1Q7Z7|DAAF3_DANRE1.490e-8434.57Dynein assembly factor 3, axonemal OS=Danio rerio ... [more]
sp|Q95K25|DAAF3_MACFA7.298e-8433.97Dynein assembly factor 3, axonemal OS=Macaca fasci... [more]
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BLAST of dynein assembly factor 3, axonemal vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3IEE12.251e-11040.58dynein assembly factor 3, axonemal OS=Lingula ungu... [more]
V4BGU51.959e-10939.36Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A3M6TVC44.492e-10637.45Uncharacterized protein OS=Pocillopora damicornis ... [more]
V9KLX23.179e-10337.17Uncharacterized protein OS=Callorhinchus milii OX=... [more]
A0A401SV255.137e-10237.58Uncharacterized protein OS=Chiloscyllium punctatum... [more]
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BLAST of dynein assembly factor 3, axonemal vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
dnaaf3l3.253e-8032.14dynein axonemal assembly factor 3 [Source:NCBI gen... [more]
dnaaf3l4.876e-7230.37dynein axonemal assembly factor 3 [Source:NCBI gen... [more]
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BLAST of dynein assembly factor 3, axonemal vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of dynein assembly factor 3, axonemal vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of dynein assembly factor 3, axonemal vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO375266.472e-4343.48Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of dynein assembly factor 3, axonemal vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
dnaaf3l2.862e-9037.28dynein axonemal assembly factor 3 [Source:NCBI gen... [more]
dnaaf3l2.862e-9037.28dynein axonemal assembly factor 3 [Source:NCBI gen... [more]
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BLAST of dynein assembly factor 3, axonemal vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000017259.10.000e+094.46SMESG000017259.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001828 ID=SMED30001828|Name=dynein assembly factor 3, axonemal|organism=Schmidtea mediterranea sexual|type=transcript|length=1513bp
TCTTAGTAACGCTTTAGTTGAAACTGAATGTCTAAAGAGAGTTTAGGTGT
TATTAATTGGTGGGGATTCACGCCAGCTCTCGATCTATTTGATAATGAAT
TGCTAGAGATTGATGAATGCACAGACAAAGCTGATATAAATATTTTATTG
GTTGGATCTGGTGACAGTCGGCATATCTTGCAGTCGCTCAGTTCTCTTTA
CAAACGGAAAAAATGCCATATTAATTTTTATATAATAGAAAACGGACTGG
AATTGTACGCGAGACAAATGCTGCAGCTGATGATCGCACTGATCCCGTTG
AACCGGATGGGATTACAAGATAAAACCGAATTATTCCTAGAATTATACGG
CAACGGCTTGGTAAGGGAGCAATCGATGGAATTTGCACGACAAGCTGCCG
TTAAATTGATCAAGTTCGTGACGAACTCGCCGGGCTTCCCCACAGCTTTA
TCTCAGCTGATTCATATCGACAACCTCAAATTCAAGGAACGCGACATTCT
GGAGGCGATTTTCAAGCTGTGGAAGGACGAAAATTCGAAATCTTTTGATC
TCAGTCTTTGCTGGGATCTGAGACTGCGTCAGAATCTGGGAACGCGCTAC
GATGCAATTCCGAATGTATTTGATTGGGATTGCTCAATCACTCTCCATGA
CCGAGGCGCTACCATTATCAATTCCCGGGAATATTCTCATTGGCGCCATA
AAGGCGTCGCGTTCCAGTTGCGAGATGCCAATTATGACGTCAGCAACAGG
ACGCTCGCATCCGGCCGGGTTTTGCAGGCCGCGGGGGGCGATAAAACGGT
CCGACTTGGATATTGGGGCGATATCGTTTGTTCTCCTTTCATTGGGTTTG
GGATAGCCAGCTCGAATGCGGCCTTGTTCCAGAGGTCAAATAATCAATTT
CTCCACGGCGCCTCGAGAGTATCCGAGTACAACATCAGTGAGATATTTCA
TCAGATCGCCTGTAACAGGAAATATGTCGAGACGAGCAATCCGTCGGCAA
CCGCCACGATAACAGAGGTAGACGAGGAACAGCAGGAGCAGCAGGAACAG
CAGGAGCAGGAGCAAATCGATGATCCTGCTCAATATCTGAACGGTGCTCC
ACGCATTTTATCGATATTATTGATAGGATTATTGATTCTAGATCTGATCG
CAGTGCCCAATGTCAAACTCATTTTCCTGCCAGTCAATTCACTGCTCGAT
TATGTGGCGAAAAAAACAAAATTTCAGTCTAATTTTGACCTTGTGTATTT
TTCCGCGAGTCTGATTAGTCAGTTAGAAGCCTGCCCGCCATTGGCGGGAA
TTTTGCGGCCGCAAGGAAAGCTATTGGTGGAGATGCCTAAATATATAGTC
AATATCAACGCGGACCAAATTGATCGGTTCCGGGCTCGAGTTGATGAGAT
GGCAAAGGCGCACTCGATGCAGGCATTTCCAGTGAACTGTGCCGCAGGAC
TGGACAAATCTGAATATGTTGTTTACACTTTGAATCAATAAATATTTTCG
TTTATTGAGATCG
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protein sequence of SMED30001828-orf-1

>SMED30001828-orf-1 ID=SMED30001828-orf-1|Name=SMED30001828-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=488bp
MSKESLGVINWWGFTPALDLFDNELLEIDECTDKADINILLVGSGDSRHI
LQSLSSLYKRKKCHINFYIIENGLELYARQMLQLMIALIPLNRMGLQDKT
ELFLELYGNGLVREQSMEFARQAAVKLIKFVTNSPGFPTALSQLIHIDNL
KFKERDILEAIFKLWKDENSKSFDLSLCWDLRLRQNLGTRYDAIPNVFDW
DCSITLHDRGATIINSREYSHWRHKGVAFQLRDANYDVSNRTLASGRVLQ
AAGGDKTVRLGYWGDIVCSPFIGFGIASSNAALFQRSNNQFLHGASRVSE
YNISEIFHQIACNRKYVETSNPSATATITEVDEEQQEQQEQQEQEQIDDP
AQYLNGAPRILSILLIGLLILDLIAVPNVKLIFLPVNSLLDYVAKKTKFQ
SNFDLVYFSASLISQLEACPPLAGILRPQGKLLVEMPKYIVNINADQIDR
FRARVDEMAKAHSMQAFPVNCAAGLDKSEYVVYTLNQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
GO:0005737cytoplasm
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR039304DNAAF3
IPR027974DUF4470
IPR028235DNAAF3_C
Vocabulary: biological process
TermDefinition
GO:0030030cell projection organization
GO:0044458motile cilium assembly
GO:0070286axonemal dynein complex assembly
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 324..344
IPR027974Domain of unknown function DUF4470PFAMPF14737DUF4470coord: 11..110
e-value: 9.0E-24
score: 83.5
IPR028235Dynein assembly factor 3, C-terminal domainPFAMPF14740DUF4471coord: 146..464
e-value: 3.6E-92
score: 308.9
IPR039304Dynein assembly factor 3, axonemalPANTHERPTHR22118FAMILY NOT NAMEDcoord: 3..465