SMED30001797
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30001797 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Alignments
SMED30001797 aligns in the following genomic locations:
Homology
BLAST of SMED30001797 vs. TrEMBL
Match: A0A0R3SZU8 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS33 PE=4 SV=1) HSP 1 Score: 114.005 bits (284), Expect = 7.776e-23 Identity = 67/182 (36.81%), Postives = 106/182 (58.24%), Query Frame = 3 Query: 321 RRLDSENXXXXXXXXXXCGSAAIILFGVALTKEGYLHQIGTGMWAGSLMLAAGCLGIAASRHRSPCTLISLLVLCVMTSVTSAILASISTAGLVQDGVKHAQST--------------------------------------------KMHLIVLHTLLFIVGFLECSVAVTTAIICGRQVC 734 RR+ +ENL +LS+IQLLCG AA++L GVA+TK +L+Q+ TG+W+G LMLA G GI +R +P TL+SLL+ CV+ ++++ +LA +S AGL++DG+ +++ ++H ++LH LL ++G LE SV+V A++C RQ+C Sbjct: 130 RRMPNENLFVLSMIQLLCGLAAVVLSGVAVTKVVFLYQMATGLWSGFLMLATGFHGIVTARRCTPRTLVSLLIFCVLVALSACLLACVSVAGLIEDGILRPETSFTLLSSNSPYYITSHSSISSNGLSVDIPLGGFSHRRVIASNQPPRLHQVMLHVLLLVIGILEASVSVACAVLCCRQIC 311
BLAST of SMED30001797 vs. TrEMBL
Match: A0A564YJB3 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS7000 PE=4 SV=1) HSP 1 Score: 112.079 bits (279), Expect = 3.107e-22 Identity = 67/183 (36.61%), Postives = 106/183 (57.92%), Query Frame = 3 Query: 321 RRLDSENXXXXXXXXXXCGSAAIILFGVALTKEGYLHQIGTGMWAGSLMLAAGCLGIAASRHRSPCTLISLLVLCVMTSVTSAILASISTAGLVQDGVKHAQST---------------------------------------------KMHLIVLHTLLFIVGFLECSVAVTTAIICGRQVC 734 RR+ +ENL +LS+IQLLCG AA++L GVA+TK +L+Q+ TG+W+G LMLA G GI +R +P TL+SLL+ CV+ ++++ +LA +S AGL++DG+ +++ ++H ++LH LL ++G LE SV+V A++C RQ+C Sbjct: 130 RRMPNENLFVLSMIQLLCGLAAVVLSGVAVTKVVFLYQMATGLWSGFLMLATGFHGILTARRCTPRTLVSLLIFCVLVALSACLLACVSVAGLIEDGILRPETSFTLFTSSNSPYYITSHSSISSNGLSVDIPLGGFSHRRVIGSNQPPRLHQVMLHVLLLVIGILEASVSVACAVLCCRQIC 312
BLAST of SMED30001797 vs. TrEMBL
Match: A0A0R3W9G3 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS7089 PE=4 SV=1) HSP 1 Score: 111.694 bits (278), Expect = 4.875e-22 Identity = 69/184 (37.50%), Postives = 104/184 (56.52%), Query Frame = 3 Query: 321 RRLDSENXXXXXXXXXXCGSAAIILFGVALTKEGYLHQIGTGMWAGSLMLAAGCLGIAASRHRSPCTLISLLVLCVMTSVTSAILASISTAGLVQDG----------------------------------------------VKHAQSTKMHLIVLHTLLFIVGFLECSVAVTTAIICGRQVC 734 RR+ +ENL +LS+IQLLCG AA++L GVA+TK +L+Q+ TG+W+G LMLA G GI +R +P TL+SLL+ CV+ ++++ +LA +S AGL++DG V Q ++H ++LH LL ++G LE SV+V A++C RQ+C Sbjct: 137 RRMPNENLFVLSMIQLLCGLAAVVLSGVAVTKVVFLYQMATGLWSGFLMLATGFHGILTARRCTPRTLVSLLIFCVLVALSACLLACVSVAGLIEDGILRPDASITLLTSSNSPFHLSSHSSSVSPSGLPVDIPLGGFSHQRFVSSNQPPRLHQVMLHILLLVIGILEASVSVACAVLCCRQIC 320
BLAST of SMED30001797 vs. TrEMBL
Match: A0A0R3S8Z6 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS674 PE=4 SV=1) HSP 1 Score: 111.309 bits (277), Expect = 5.571e-22 Identity = 67/183 (36.61%), Postives = 106/183 (57.92%), Query Frame = 3 Query: 321 RRLDSENXXXXXXXXXXCGSAAIILFGVALTKEGYLHQIGTGMWAGSLMLAAGCLGIAASRHRSPCTLISLLVLCVMTSVTSAILASISTAGLVQDGVKHAQST---------------------------------------------KMHLIVLHTLLFIVGFLECSVAVTTAIICGRQVC 734 RR+ +ENL +LS+IQLLCG AA++L GVA+TK +L+Q+ TG+W+G LMLA G GI +R +P TL+SLL+ CV+ ++++ +LA +S AGL++DG+ +++ ++H ++LH LL ++G LE SV+V A++C RQ+C Sbjct: 130 RRMPNENLFVLSMIQLLCGLAAVVLSGVAVTKVVFLYQMATGLWSGFLMLATGFHGILTARRCTPRTLVSLLIFCVLVALSACLLACVSVAGLIEDGILRPETSFTLFTSSNSPYYITSHSSISSNGLSVDIPLGGFSHRRVIGSNQPPRLHQVMLHVLLLVIGILEASVSVACAVLCCRQIC 312
BLAST of SMED30001797 vs. TrEMBL
Match: A0A068WQ39 (Uncharacterized protein OS=Echinococcus granulosus OX=6210 GN=EgrG_000076500 PE=4 SV=1) HSP 1 Score: 111.309 bits (277), Expect = 6.322e-22 Identity = 69/184 (37.50%), Postives = 104/184 (56.52%), Query Frame = 3 Query: 321 RRLDSENXXXXXXXXXXCGSAAIILFGVALTKEGYLHQIGTGMWAGSLMLAAGCLGIAASRHRSPCTLISLLVLCVMTSVTSAILASISTAGLVQDG----------------------------------------------VKHAQSTKMHLIVLHTLLFIVGFLECSVAVTTAIICGRQVC 734 RR+ +ENL +LS+IQLLCG AA++L GVA+TK +L+Q+ TG+W+G LMLA G GI +R +P TL+SLL+ CV+ ++++ +LA +S AGL++DG V Q ++H ++LH LL ++G LE SV+V A++C RQ+C Sbjct: 137 RRMPNENLFVLSMIQLLCGLAAVVLSGVAVTKVVFLYQMATGLWSGFLMLATGFHGILTARRCTPRTLVSLLIFCVLVALSACLLACVSVAGLIEDGILRPDTSITLFTASNSPFYLSSHGPSVASSSLPIDIPLGGFSHRRLVSSNQPPRLHQVMLHILLLVIGILEASVSVACAVLCCRQIC 320
BLAST of SMED30001797 vs. Planmine SMEST
Match: SMESG000034362.1 (SMESG000034362.1) HSP 1 Score: 362.459 bits (929), Expect = 5.534e-124 Identity = 190/191 (99.48%), Postives = 190/191 (99.48%), Query Frame = 3 Query: 246 MTPQQSACLPLSENRKLCVCCPHLPRRLDSENXXXXXXXXXXCGSAAIILFGVALTKEGYLHQIGTGMWAGSLMLAAGCLGIAASRHRSPCTLISLLVLCVMTSVTSAILASISTAGLVQDGVKHAQSTKMHLIVLHTLLFIVGFLECSVAVTTAIICGRQVCFGAVYLPAGSSVRRRACSGFRRGSIYGR 818 MTPQQSACLPLSENRKLCVCCPHLPRRLDSENLLILSLIQLLCGSAAIILFGVALTKEGYLHQIGTGMWAGSLMLAAGCLGIA SRHRSPCTLISLLVLCVMTSVTSAILASISTAGLVQDGVKHAQSTKMHLIVLHTLLFIVGFLECSVAVTTAIICGRQVCFGAVYLPAGSSVRRRACSGFRRGSIYGR Sbjct: 1 MTPQQSACLPLSENRKLCVCCPHLPRRLDSENLLILSLIQLLCGSAAIILFGVALTKEGYLHQIGTGMWAGSLMLAAGCLGIATSRHRSPCTLISLLVLCVMTSVTSAILASISTAGLVQDGVKHAQSTKMHLIVLHTLLFIVGFLECSVAVTTAIICGRQVCFGAVYLPAGSSVRRRACSGFRRGSIYGR 191
BLAST of SMED30001797 vs. Planmine SMEST
Match: SMESG000043901.1 (SMESG000043901.1) HSP 1 Score: 322.398 bits (825), Expect = 4.895e-107 Identity = 173/180 (96.11%), Postives = 173/180 (96.11%), Query Frame = 3 Query: 246 MTPQQSACLPLSENRKLCVCCPHLPRRLDSENXXXXXXXXXXCGSAAIILFGVALTKEGYLHQIGTGMWAGSLMLAAGCLGIAASRHRSPCTLISLLVLCVMTSVTSAILASISTAGLVQDGVKHAQSTKMHLIVLHTLLFIVGFLECSVAVTTAIICGRQVCFGAVYLPAGSSVRRRAC 785 MTPQQSACLPLSENRKLCVCCPHLPRRLDSENLLILSLIQLLCGSAAIILFGVALTKEGYLHQIGTGMWAGSLMLAAGCLGIA SRHRSPCTLISLLVLCVMTSVTSAILASISTAGLVQDGVKHAQSTKMHLIVLHTLLFIVGFLECSVAVTTAIICGRQVCFGAVYLPA RAC Sbjct: 1 MTPQQSACLPLSENRKLCVCCPHLPRRLDSENLLILSLIQLLCGSAAIILFGVALTKEGYLHQIGTGMWAGSLMLAAGCLGIATSRHRSPCTLISLLVLCVMTSVTSAILASISTAGLVQDGVKHAQSTKMHLIVLHTLLFIVGFLECSVAVTTAIICGRQVCFGAVYLPA-----ERAC 175 HSP 2 Score: 144.436 bits (363), Expect = 6.130e-39 Identity = 98/100 (98.00%), Postives = 98/100 (98.00%), Query Frame = 3 Query: 1095 PPFPPSYSVQESRPLSAYACVNATDSGDTLLQPVLLVEEQPPPYDSLEYLPDSKEXXXXXXXXXXXXXXXXEPNVSMLVSQPSPATTXXXXXXXXXWKPV 1394 P PPSYSVQESRPLSAYACVNATDSGDTLLQPVLLVEEQPPPYDSLEYLPDSKEVDNDLHSLHDDVVVLVEPNVSMLVSQPSPATTSSSSSSSSSWKPV Sbjct: 179 PSIPPSYSVQESRPLSAYACVNATDSGDTLLQPVLLVEEQPPPYDSLEYLPDSKEVDNDLHSLHDDVVVLVEPNVSMLVSQPSPATTSSSSSSSSSWKPV 278
BLAST of SMED30001797 vs. Planmine SMEST
Match: SMESG000034363.1 (SMESG000034363.1) HSP 1 Score: 229.95 bits (585), Expect = 2.879e-72 Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 3 Query: 924 RNSGQLPVLTTNSFYANVLFPQLQSSPHAQSPLSATCRSSLVYLLPSPNQNEPALLFPPFPPSYSVQESRPLSAYACVNATDSGDTLLQPVLLVEEQPPPYDSLEYLPDSKEXXXXXXXXXXXXXXXXEPNVSMLVSQPSPATTXXXXXXXXXWKPV 1394 RNSGQLPVLTTNSFYANVLFPQLQSSPHAQSPLSATCRSSLVYLLPSPNQNEPALLFPPFPPSYSVQESRPLSAYACVNATDSGDTLLQPVLLVEEQPPPYDSLEYLPDSKEVDNDLHSLHDDVVVLVEPNVSMLVSQPSPATTSSSSSSSSSWKPV Sbjct: 41 RNSGQLPVLTTNSFYANVLFPQLQSSPHAQSPLSATCRSSLVYLLPSPNQNEPALLFPPFPPSYSVQESRPLSAYACVNATDSGDTLLQPVLLVEEQPPPYDSLEYLPDSKEVDNDLHSLHDDVVVLVEPNVSMLVSQPSPATTSSSSSSSSSWKPV 197 The following BLAST results are available for this feature:
BLAST of SMED30001797 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30001797 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30001797 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30001797 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30001797 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30001797 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30001797 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30001797 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30001797 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30001797 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30001797 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30001797 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30001797 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30001797 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30001797 ID=SMED30001797|Name=SMED30001797|organism=Schmidtea mediterranea sexual|type=transcript|length=1686bpback to top protein sequence of SMED30001797-orf-1 >SMED30001797-orf-1 ID=SMED30001797-orf-1|Name=SMED30001797-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=384bp MTPQQSACLPLSENRKLCVCCPHLPRRLDSENLLILSLIQLLCGSAAIILback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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