SMED30001783

Overview
NameSMED30001783
Smed IDSMED30001783
Length (bp)767
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30001783 (SMED30001783) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30001783 (SMED30001783) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30001783 (SMED30001783) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30001783 (SMED30001783) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30001783

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30001783SMESG000040740.1 SmedASXL_005833SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30001783SMESG000040740.1 dd_Smed_v4_3289_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30001783SMESG000040740.1 dd_Smed_v4_3289_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001783SMESG000040740.1 Contig39484newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001783SMESG000040740.1 Contig39484uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30001783 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.020572:619..1423 -3701
Homology
[Close]
BLAST of SMED30001783 vs. Ensembl Human
Match: MRPL17 (mitochondrial ribosomal protein L17 [Source:HGNC Symbol;Acc:HGNC:14053])

HSP 1 Score: 74.3294 bits (181), Expect = 8.649e-16
Identity = 44/122 (36.07%), Postives = 66/122 (54.10%), Query Frame = -1 
Query:  117 VGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPI 482
            +G G   ++ L+R  +T L+  ERIE       E R YAE+LI    + G      + + +FWL E  L+ K+F+ L PRY+  T  +TR+ ++P  RS  + K     AV+E KGN  PP+
Sbjct:   18 MGLGPESRIHLLRNLLTGLVRHERIEAPWARVDEMRGYAEKLIDYGKL-GDTNERAMRMADFWLTEKDLIPKLFQVLAPRYKDQTGGYTRMLQIP-NRSLDRAKM----AVIEYKGNCLPPL 133
[Close]
BLAST of SMED30001783 vs. Ensembl Human
Match: MRPL17 (mitochondrial ribosomal protein L17 [Source:HGNC Symbol;Acc:HGNC:14053])

HSP 1 Score: 54.6842 bits (130), Expect = 7.787e-9
Identity = 42/154 (27.27%), Postives = 69/154 (44.81%), Query Frame = -1 
Query:   21 VGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPIKTRYQALKSQNKSQFLTNILVSAAKEEWKTNK 482
            +G G   ++ L+R  +T L+  ERIE       E R YAE+                        E  L+ K+F+ L PRY+  T  +TR+ ++P  RS  + K     AV+E KGN  PP+      L  ++    L N L+   +++ + ++
Sbjct:   18 MGLGPESRIHLLRNLLTGLVRHERIEAPWARVDEMRGYAEK------------------------EKDLIPKLFQVLAPRYKDQTGGYTRMLQIP-NRSLDRAKM----AVIEYKGNCLPPL-----PLPRRDSHLTLLNQLLQGLRQDLRQSQ 137
[Close]
BLAST of SMED30001783 vs. Ensembl Celegans
Match: mrpl-17 (Mitochondrial Ribosomal Protein, Large [Source:UniProtKB/TrEMBL;Acc:Q9N3F3])

HSP 1 Score: 96.6709 bits (239), Expect = 2.089e-24
Identity = 59/181 (32.60%), Postives = 98/181 (54.14%), Query Frame = -1 
Query:   45 CSSAKIGNAAYQLKFNIGDKENKIYYGEGVGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIK-----FPDHN-AVLELKGNSFPPIKTRYQALKSQNKSQFLTNILVSAA 569
             S+ ++  +  ++   IG    K+  G G+      ++ ++RR VT  + EER ELK   A+E R Y ERLIQ  + +GP   +  E+ME+WL E  L+ KM E +VPR++     FT LF+LP  R +  I+     +  ++  VLE+ GN FP I+         ++S+ + ++L++ A
Sbjct:    1 MSAHRVAVSLPRIGVTIGHAPQKLKTG-GIEPSRRARLEVLRRIVTRTVREERAELKWNRAVEARPYLERLIQLGVERGPLDEYTAEMMEWWLPEKDLITKMHEVIVPRFQDRDSPFTSLFRLPPQRLQQFIQNKREVWKRYDIGVLEIDGNPFPQIQA-----DRVDQSESILDVLLADA 175
[Close]
BLAST of SMED30001783 vs. Ensembl Celegans
Match: mrpl-17 (Mitochondrial Ribosomal Protein, Large [Source:UniProtKB/TrEMBL;Acc:Q9N3F3])

HSP 1 Score: 96.6709 bits (239), Expect = 2.089e-24
Identity = 59/181 (32.60%), Postives = 98/181 (54.14%), Query Frame = -1 
Query:   45 CSSAKIGNAAYQLKFNIGDKENKIYYGEGVGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIK-----FPDHN-AVLELKGNSFPPIKTRYQALKSQNKSQFLTNILVSAA 569
             S+ ++  +  ++   IG    K+  G G+      ++ ++RR VT  + EER ELK   A+E R Y ERLIQ  + +GP   +  E+ME+WL E  L+ KM E +VPR++     FT LF+LP  R +  I+     +  ++  VLE+ GN FP I+         ++S+ + ++L++ A
Sbjct:    1 MSAHRVAVSLPRIGVTIGHAPQKLKTG-GIEPSRRARLEVLRRIVTRTVREERAELKWNRAVEARPYLERLIQLGVERGPLDEYTAEMMEWWLPEKDLITKMHEVIVPRFQDRDSPFTSLFRLPPQRLQQFIQNKREVWKRYDIGVLEIDGNPFPQIQA-----DRVDQSESILDVLLADA 175
[Close]
BLAST of SMED30001783 vs. Ensembl Celegans
Match: mrpl-17 (Mitochondrial Ribosomal Protein, Large [Source:UniProtKB/TrEMBL;Acc:Q9N3F3])

HSP 1 Score: 96.6709 bits (239), Expect = 2.089e-24
Identity = 59/181 (32.60%), Postives = 98/181 (54.14%), Query Frame = -1 
Query:   45 CSSAKIGNAAYQLKFNIGDKENKIYYGEGVGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIK-----FPDHN-AVLELKGNSFPPIKTRYQALKSQNKSQFLTNILVSAA 569
             S+ ++  +  ++   IG    K+  G G+      ++ ++RR VT  + EER ELK   A+E R Y ERLIQ  + +GP   +  E+ME+WL E  L+ KM E +VPR++     FT LF+LP  R +  I+     +  ++  VLE+ GN FP I+         ++S+ + ++L++ A
Sbjct:    1 MSAHRVAVSLPRIGVTIGHAPQKLKTG-GIEPSRRARLEVLRRIVTRTVREERAELKWNRAVEARPYLERLIQLGVERGPLDEYTAEMMEWWLPEKDLITKMHEVIVPRFQDRDSPFTSLFRLPPQRLQQFIQNKREVWKRYDIGVLEIDGNPFPQIQA-----DRVDQSESILDVLLADA 175
[Close]
BLAST of SMED30001783 vs. Ensembl Fly
Match: mRpL17 (gene:FBgn0035122 transcript:FBtr0072503)

HSP 1 Score: 99.3673 bits (246), Expect = 1.726e-25
Identity = 59/148 (39.86%), Postives = 89/148 (60.14%), Query Frame = -1 
Query:   21 GQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPIKTRYQALKSQNKSQFLTNILVSAAKEEWKTNK 464
            G++  +R+ VT+L+  ERIEL +  A E R YAE LI  AI  G      +EL ++WL E +LV K+F+ LVPRY    +S+TR++K   PR    I +    +VLEL+GN +P +     A  SQN++  L N+L+  A++E++  K
Sbjct:   32 GRLLKLRKTVTALVKHERIELFYNRADEARGYAELLISNAIRHGDRHQATMELADYWLLEKQLVHKLFKVLVPRYETYNVSYTRMYK--APREYPGIYY--RRSVLELRGNPYPSL----AADHSQNRN-LLHNVLLDEARKEFRRQK 170
[Close]
BLAST of SMED30001783 vs. Ensembl Fly
Match: mRpL17 (gene:FBgn0035122 transcript:FBtr0310415)

HSP 1 Score: 99.3673 bits (246), Expect = 1.726e-25
Identity = 59/148 (39.86%), Postives = 89/148 (60.14%), Query Frame = -1 
Query:   21 GQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPIKTRYQALKSQNKSQFLTNILVSAAKEEWKTNK 464
            G++  +R+ VT+L+  ERIEL +  A E R YAE LI  AI  G      +EL ++WL E +LV K+F+ LVPRY    +S+TR++K   PR    I +    +VLEL+GN +P +     A  SQN++  L N+L+  A++E++  K
Sbjct:   32 GRLLKLRKTVTALVKHERIELFYNRADEARGYAELLISNAIRHGDRHQATMELADYWLLEKQLVHKLFKVLVPRYETYNVSYTRMYK--APREYPGIYY--RRSVLELRGNPYPSL----AADHSQNRN-LLHNVLLDEARKEFRRQK 170
[Close]
BLAST of SMED30001783 vs. Ensembl Zebrafish
Match: mrpl17 (mitochondrial ribosomal protein L17 [Source:ZFIN;Acc:ZDB-GENE-040912-133])

HSP 1 Score: 70.4774 bits (171), Expect = 1.235e-14
Identity = 43/122 (35.25%), Postives = 66/122 (54.10%), Query Frame = -1 
Query:  117 VGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPI 482
            +G G   ++ ++R  +T L+  ERIE     A E R YAE+LI  A  KG      +++ +FWL E  L+ K+F+ L  R+ + T  +T + +LP   +  +       AVLE KGN FPP+
Sbjct:   18 MGLGPESRINILRNLLTGLVRHERIETGKARADEVRFYAEKLIDYAK-KGDTDEKAMKMADFWLTEKDLIPKLFKVLAVRFENQTAGYTNMARLPNRENLDRAAM----AVLEYKGNPFPPL 134
[Close]
BLAST of SMED30001783 vs. Ensembl Xenopus
Match: myom3 (myomesin 3 [Source:Xenbase;Acc:XB-GENE-490325])

HSP 1 Score: 73.559 bits (179), Expect = 1.462e-15
Identity = 46/142 (32.39%), Postives = 75/142 (52.82%), Query Frame = -1 
Query:   57 VGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPIKTRYQALKSQNKSQFLTNIL 482
            +G G   ++ L+R  +T L   ERIE     A E R+YAE+LI     +G      +++ +FWL E  L+ K+F+ L PR+     ++TR++++P   +  + K     AV+E KGN +PP+ T     K  N+   L  +L
Sbjct:   18 LGLGPRSRLDLLRNLLTGLTRHERIETTWARADELRQYAEKLIDYGK-RGDTDERAMKMADFWLTEKDLIPKLFKVLAPRFSSHHSNYTRMYQIPSRDNVDRAKM----AVIEYKGNPYPPLITP----KKDNEKTLLNQLL 150
[Close]
BLAST of SMED30001783 vs. Ensembl Mouse
Match: Mrpl17 (mitochondrial ribosomal protein L17 [Source:MGI Symbol;Acc:MGI:1351608])

HSP 1 Score: 73.559 bits (179), Expect = 1.122e-15
Identity = 41/122 (33.61%), Postives = 67/122 (54.92%), Query Frame = -1 
Query:  117 VGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPI 482
            +G G   ++ L+R  +T L+  ERIE     A E R YAE+LI    + G      + + +FWL E  L+ K+F+ L PR++    ++TR+ ++P  + + + K     AV+E KGN  PP+
Sbjct:   18 LGLGPESRIHLLRNLLTGLVRHERIEATWARADEMRGYAEKLIDYGKL-GDTNERAMRMADFWLTEKDLIPKLFKVLAPRFQGQNGNYTRMLQIPNRKEQDRAKM----AVIEYKGNYLPPL 134
[Close]
BLAST of SMED30001783 vs. Ensembl Mouse
Match: Mrpl17 (mitochondrial ribosomal protein L17 [Source:MGI Symbol;Acc:MGI:1351608])

HSP 1 Score: 51.2174 bits (121), Expect = 5.167e-8
Identity = 22/66 (33.33%), Postives = 40/66 (60.61%), Query Frame = -1 
Query:  117 IELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPI 314
            + + +FWL E  L+ K+F+ L PR++    ++TR+ ++P  + + + K     AV+E KGN  PP+
Sbjct:   19 MRMADFWLTEKDLIPKLFKVLAPRFQGQNGNYTRMLQIPNRKEQDRAKM----AVIEYKGNYLPPL 80
[Close]
BLAST of SMED30001783 vs. UniProt/SwissProt
Match: sp|Q9NRX2|RM17_HUMAN (39S ribosomal protein L17, mitochondrial OS=Homo sapiens OX=9606 GN=MRPL17 PE=1 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 4.154e-15
Identity = 44/122 (36.07%), Postives = 66/122 (54.10%), Query Frame = -1 
Query:  117 VGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPI 482
            +G G   ++ L+R  +T L+  ERIE       E R YAE+LI    + G      + + +FWL E  L+ K+F+ L PRY+  T  +TR+ ++P  RS  + K     AV+E KGN  PP+
Sbjct:   18 MGLGPESRIHLLRNLLTGLVRHERIEAPWARVDEMRGYAEKLIDYGKL-GDTNERAMRMADFWLTEKDLIPKLFQVLAPRYKDQTGGYTRMLQIP-NRSLDRAKM----AVIEYKGNCLPPL 133
[Close]
BLAST of SMED30001783 vs. UniProt/SwissProt
Match: sp|Q9D8P4|RM17_MOUSE (39S ribosomal protein L17, mitochondrial OS=Mus musculus OX=10090 GN=Mrpl17 PE=1 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 7.848e-15
Identity = 41/122 (33.61%), Postives = 67/122 (54.92%), Query Frame = -1 
Query:  117 VGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPI 482
            +G G   ++ L+R  +T L+  ERIE     A E R YAE+LI    + G      + + +FWL E  L+ K+F+ L PR++    ++TR+ ++P  + + + K     AV+E KGN  PP+
Sbjct:   18 LGLGPESRIHLLRNLLTGLVRHERIEATWARADEMRGYAEKLIDYGKL-GDTNERAMRMADFWLTEKDLIPKLFKVLAPRFQGQNGNYTRMLQIPNRKEQDRAKM----AVIEYKGNYLPPL 134
[Close]
BLAST of SMED30001783 vs. UniProt/SwissProt
Match: sp|Q3T0L3|RM17_BOVIN (39S ribosomal protein L17, mitochondrial OS=Bos taurus OX=9913 GN=MRPL17 PE=1 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.932e-14
Identity = 43/140 (30.71%), Postives = 70/140 (50.00%), Query Frame = -1 
Query:  117 QLKFNIGDKENKIYYGEGVGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPI 536
            +L F       ++Y   G+G     ++ L++  +T L+  ERIE       E R YAE+LI    + G      + + +FWL E  L+ K+F+ L PRY+     +TR+ ++P    + + K     AV+E KGN  PP+
Sbjct:    2 RLSFAAAISHGRVYRRLGLGPE--SRIHLLQNLLTGLVRHERIEASWARVDELRGYAEKLIDYGKL-GDTNERAMRMADFWLTEKDLIPKLFQVLAPRYQGQNGGYTRMLQIPNRNQQDRAKM----AVIEYKGNCLPPL 134
[Close]
BLAST of SMED30001783 vs. UniProt/SwissProt
Match: sp|Q6PDW6|RM17_RAT (39S ribosomal protein L17, mitochondrial OS=Rattus norvegicus OX=10116 GN=Mrpl17 PE=2 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.470e-14
Identity = 40/122 (32.79%), Postives = 66/122 (54.10%), Query Frame = -1 
Query:  117 VGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPI 482
            +G G   ++ L+R  +T L+  ERIE       E R YAE+LI    + G      + + +FWL E  L+ K+F+ L PR++    ++TR+ ++P  + + + K     AV+E KGN  PP+
Sbjct:   18 LGLGPESRIHLLRNLLTGLVRHERIEATWARVDEMRGYAEKLIDYGKL-GDTNERAMRMADFWLTEKDLIPKLFKVLAPRFQGQNGNYTRMLQIPNRKEQDRAKM----AVIEYKGNCLPPL 134
[Close]
BLAST of SMED30001783 vs. UniProt/SwissProt
Match: sp|Q5RCA3|RM17_PONAB (39S ribosomal protein L17, mitochondrial OS=Pongo abelii OX=9601 GN=MRPL17 PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 6.932e-14
Identity = 43/122 (35.25%), Postives = 65/122 (53.28%), Query Frame = -1 
Query:  117 VGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPI 482
            +G G   ++ L+R  +T L+  ERIE       E R YAE+LI    + G      + + +FWL E  L+ K+F+ L PRY+     +TR+ ++P  RS  + K     AV+E KGN  PP+
Sbjct:   18 MGLGPESRIHLLRNLLTGLVRHERIEAPWARVDEMRGYAEKLIDYGKL-GDTNERAMRMADFWLTEKDLIPKLFQVLAPRYKDQNGGYTRMRQIP-NRSLDRAKM----AVIEYKGNCLPPL 133
[Close]
BLAST of SMED30001783 vs. TrEMBL
Match: A0A4Z2DV10 (39S ribosomal protein L17 OS=Schistosoma japonicum OX=6182 GN=EWB00_005470 PE=4 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 9.568e-32
Identity = 84/217 (38.71%), Postives = 116/217 (53.46%), Query Frame = -1 
Query:   39 FRKGI-----IRNCSS-AKIGNAAYQLKFNIGDKENKIYYGEGVGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAII--------------KGPNCP-----FNIELME---FWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPIKTRYQALKSQN----KSQFLTNILVSAAKE 593
            FR GI      RN S+    G AA +L+F +  K  ++   EGVGS   G+M L+RR V+ L+D+ERIEL   HA+ETR YAERLIQE ++              K  N P      N+ ++E   FWL E +LV+K+F+  VPRYR    ++T LFK+  P    K        +LEL GN +PP+     A +  N    K  +L N+L+ AA+E
Sbjct:   40 FRFGISLVQLCRNASNQTDYGPAATRLRFPVPLKARRMRRAEGVGSDVTGRMMLLRRYVSKLLDDERIELPWPHAVETRLYAERLIQEGVLASTQQNLVSLFELWKNSNEPKIDGKLNVGILELSVFWLTEERLVEKLFKVFVPRYRLYQRAYTSLFKIFSPVYPIKTSGTKAFGILELHGNPWPPVHGHGIAGRDLNPEPFKHNYLINVLLDAARE 256
[Close]
BLAST of SMED30001783 vs. TrEMBL
Match: A0A5K4FCH8 (Putative mitochondrial ribosomal protein L17 OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 4.077e-31
Identity = 77/197 (39.09%), Postives = 110/197 (55.84%), Query Frame = -1 
Query:   39 GNAAYQLKFNIGDKENKIYYGEGVGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAII-----------------KGP--NCPFNIELME---FWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPIKTRYQALKSQN----KSQFLTNILVSAAKE 551
            G AA +L+F +  +  ++   EGVG G  G+M L+RR V+ L+D+ERIEL   HA+ETR YAERLIQE I+                 K P  N   N+ ++E   FWL E +LV+K+F+  VPRYR    S+T LF++  P    K        +LEL GN +PP+     A +S N    + ++L N+L+ AA+E
Sbjct:   43 GAAATRLRFPVSSRPIRLKRAEGVGGGVTGRMMLLRRYVSKLLDDERIELPWSHAIETRLYAERLIQEGILASAEQDLVSLFELWKNSKEPEINDKLNLGILELSVFWLTEERLVEKLFKVFVPRYRFYGRSYTSLFQIFPPVYPIKPCGTKSFGILELHGNPWPPVHGHGIAGRSLNPEPFQDRYLINVLLDAARE 239
[Close]
BLAST of SMED30001783 vs. TrEMBL
Match: A0A183NS79 (Uncharacterized protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS4965 PE=4 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 4.339e-31
Identity = 84/228 (36.84%), Postives = 123/228 (53.95%), Query Frame = -1 
Query:   39 RLCTFRKGIIRNC-----SSAKIGN---AAYQLKFNIGDKENKIYYGEGVGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAII-----------------KGP--NCPFNIELME---FWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKL-----PIPRSETKIKFPDHNAVLELKGNSFPPIKTRYQALKSQN----KSQFLTNILVSAAKE 605
            RL  F   +++ C      S K+G+   A  +L+F +  +  ++   EGVG G  G+M L+RR V+ L D+ERIEL   HA ETR YAERLIQE+I+                 K P  N   N+ ++E   FWL E +LV+K+F+  VPRYR    S+T LF++     PI  S TK+       +LEL GN +PP++      +S N    + ++L N+L+ AA+E
Sbjct:   21 RLLRFGTSLVQLCRKSSNQSGKVGDYGAAVTRLRFPVSSRPIRMRRAEGVGGGVTGRMMLLRRYVSKLFDDERIELPWPHATETRLYAERLIQESILASAEQDLVSLFELWKNSKEPKTNDKLNLGILELSVFWLTEERLVEKLFKVFVPRYRFYERSYTSLFQIFPPVHPIKSSGTKL-----FGILELHGNPWPPVRGHGIIGRSLNPEPFQDRYLINVLLDAARE 243
[Close]
BLAST of SMED30001783 vs. TrEMBL
Match: A0A430QRM8 (Large subunit ribosomal protein L17 OS=Schistosoma bovis OX=6184 GN=DC041_0006823 PE=4 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 5.964e-31
Identity = 80/223 (35.87%), Postives = 120/223 (53.81%), Query Frame = -1 
Query:   39 RLCTFRKGIIRNC-----SSAKIGN---AAYQLKFNIGDKENKIYYGEGVGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAII-----------------KGP--NCPFNIELME---FWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPIKTRYQALKSQN----KSQFLTNILVSAAKE 605
            RL  F   +++ C      S K+G+   A  +L+F +  +  ++   EGVG G  G+M L+RR V+ L+D+ERIEL   HA ETR YAERLIQE+I+                 K P  N   N+ ++E   FWL E +LV+K+F+  VPRYR    ++T LF++  P    K        +LEL GN +PP++      +S N    + ++L N+L+ AA+E
Sbjct:   21 RLLRFGTSLVQLCRKSSNQSGKVGDYGAAVTRLRFPVSPRPIRMRRAEGVGGGVTGRMMLLRRYVSKLLDDERIELPWPHATETRLYAERLIQESILASAEQDLVSLFELWKNSKEPKTNDKLNLGILELSVFWLTEERLVEKLFKVFVPRYRFYERAYTSLFQIFPPVHPIKSSGTKSFGILELHGNPWPPVRGHGIIGRSLNPEPFQDRYLINVLLDAARE 243
[Close]
BLAST of SMED30001783 vs. TrEMBL
Match: A0A183K667 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS10492 PE=4 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 6.423e-31
Identity = 80/223 (35.87%), Postives = 120/223 (53.81%), Query Frame = -1 
Query:   39 RLCTFRKGIIRNC-----SSAKIGN---AAYQLKFNIGDKENKIYYGEGVGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAII-----------------KGP--NCPFNIELME---FWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPIKTRYQALKSQN----KSQFLTNILVSAAKE 605
            RL  F   +++ C      S K+G+   A  +L+F +  +  ++   EGVG G  G+M L+RR V+ L+D+ERIEL   HA ETR YAERLIQE+I+                 K P  N   N+ ++E   FWL E +LV+K+F+  VPRYR    ++T LF++  P    K        +LEL GN +PP++      +S N    + ++L N+L+ AA+E
Sbjct:   21 RLLRFGTSLVQLCRKPSNQSGKVGDYGAAVTRLRFPVSPRPIRMRRAEGVGGGVTGRMMLLRRYVSKLLDDERIELPWPHATETRLYAERLIQESILASAEQDLVSLFELWKNSKEPKTNDKLNLGILELSVFWLTEERLVEKLFKVFVPRYRFYERAYTSLFQIFPPVHPIKSSGTKSFGILELHGNPWPPVRGHGIIGRSLNPEPFQDRYLINVLLDAARE 243
[Close]
BLAST of SMED30001783 vs. Ensembl Cavefish
Match: mrpl17 (mitochondrial ribosomal protein L17 [Source:ZFIN;Acc:ZDB-GENE-040912-133])

HSP 1 Score: 71.2478 bits (173), Expect = 6.544e-15
Identity = 47/153 (30.72%), Postives = 80/153 (52.29%), Query Frame = -1 
Query:   24 VGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPIKTRYQALKSQNKSQFLTNILVSAAKEEWKTN 482
            +G G   ++ ++R  +T L+  ERIE     A E + YAE+LI  A  KG     ++++ +FWL E  L+ K+F+ L PR+ +    +TR+ +LP   +  +       AV+E KGN FPP+      +K  N+   +  +L    +E+ + N
Sbjct:   18 MGLGPESRINMLRNILTGLVRHERIETTRVRADEVQFYAEKLIDYAK-KGDTNEKSMKMADFWLTEKDLIPKLFKVLAPRFENQPSGYTRMARLPNRENLDRAAM----AVIEYKGNPFPPL----FPVKQDNELNLVNQLLKGYREEQAQLN 161
[Close]
BLAST of SMED30001783 vs. Ensembl Nematostella
Match: EDO32208 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SVP4])

HSP 1 Score: 48.9062 bits (115), Expect = 9.774e-8
Identity = 34/114 (29.82%), Postives = 57/114 (50.00%), Query Frame = -1 
Query:  117 LMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELM--EFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPI 452
            L +  +T+L+  ERIE       +  +  ER+I  A  +G     N  LM   ++L    LV K+F +L+PRY   T  +T+++++P PR     +      ++EL GN  P +
Sbjct:    6 LYKNLITALVQHERIETTLTRCQDLARVTERMIDLAK-QGDEETVNSWLMVSGYFLVNKDLVPKVFAELLPRYEGITKGYTQVYRIP-PRKTDSAQM----GIVELVGNDLPQL 113
[Close]
BLAST of SMED30001783 vs. Ensembl Medaka
Match: mrpl17 (mitochondrial ribosomal protein L17 [Source:NCBI gene;Acc:101161504])

HSP 1 Score: 75.8702 bits (185), Expect = 1.011e-16
Identity = 47/122 (38.52%), Postives = 67/122 (54.92%), Query Frame = -1 
Query:  117 VGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPI 482
            +G G   ++ ++R  +T L+  ERIE     A E R YAE+LI  A  KG      +++  FWL E  LV K+F+ L PR+   +  FTR+ +LP   +  + K     AVLE KGN FPP+
Sbjct:   18 MGLGPESRINMLRNILTGLVRHERIETTLARADEVRFYAEKLIDYAK-KGDTDEKAMKMASFWLTEKDLVPKLFKVLAPRFEKHSQGFTRMARLPNRENLDRAKM----AVLEYKGNPFPPL 134
[Close]
BLAST of SMED30001783 vs. Planmine SMEST
Match: SMESG000040740.1 (SMESG000040740.1)

HSP 1 Score: 411.379 bits (1056), Expect = 6.476e-148
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = -1 
Query:   21 MFKRLCTFRKGIIRNCSSAKIGNAAYQLKFNIGDKENKIYYGEGVGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPIKTRYQALKSQNKSQFLTNILVSAAKEEWKTNK 614
            MFKRLCTFRKGIIRNCSSAKIGNAAYQLKFNIGDKENKIYYGEGVGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPIKTRYQALKSQNKSQFLTNILVSAAKEEWKTNK
Sbjct:    1 MFKRLCTFRKGIIRNCSSAKIGNAAYQLKFNIGDKENKIYYGEGVGSGYIGQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFNIELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFPDHNAVLELKGNSFPPIKTRYQALKSQNKSQFLTNILVSAAKEEWKTNK 198
The following BLAST results are available for this feature:
BLAST of SMED30001783 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
MRPL178.649e-1636.07mitochondrial ribosomal protein L17 [Source:HGNC S... [more]
MRPL177.787e-927.27mitochondrial ribosomal protein L17 [Source:HGNC S... [more]
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BLAST of SMED30001783 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
mrpl-172.089e-2432.60Mitochondrial Ribosomal Protein, Large [Source:Un... [more]
mrpl-172.089e-2432.60Mitochondrial Ribosomal Protein, Large [Source:Un... [more]
mrpl-172.089e-2432.60Mitochondrial Ribosomal Protein, Large [Source:Un... [more]
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BLAST of SMED30001783 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mRpL171.726e-2539.86gene:FBgn0035122 transcript:FBtr0072503[more]
mRpL171.726e-2539.86gene:FBgn0035122 transcript:FBtr0310415[more]
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BLAST of SMED30001783 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl171.235e-1435.25mitochondrial ribosomal protein L17 [Source:ZFIN;A... [more]
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BLAST of SMED30001783 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
myom31.462e-1532.39myomesin 3 [Source:Xenbase;Acc:XB-GENE-490325][more]
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BLAST of SMED30001783 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Mrpl171.122e-1533.61mitochondrial ribosomal protein L17 [Source:MGI Sy... [more]
Mrpl175.167e-833.33mitochondrial ribosomal protein L17 [Source:MGI Sy... [more]
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BLAST of SMED30001783 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9NRX2|RM17_HUMAN4.154e-1536.0739S ribosomal protein L17, mitochondrial OS=Homo s... [more]
sp|Q9D8P4|RM17_MOUSE7.848e-1533.6139S ribosomal protein L17, mitochondrial OS=Mus mu... [more]
sp|Q3T0L3|RM17_BOVIN1.932e-1430.7139S ribosomal protein L17, mitochondrial OS=Bos ta... [more]
sp|Q6PDW6|RM17_RAT2.470e-1432.7939S ribosomal protein L17, mitochondrial OS=Rattus... [more]
sp|Q5RCA3|RM17_PONAB6.932e-1435.2539S ribosomal protein L17, mitochondrial OS=Pongo ... [more]
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BLAST of SMED30001783 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4Z2DV109.568e-3238.7139S ribosomal protein L17 OS=Schistosoma japonicum... [more]
A0A5K4FCH84.077e-3139.09Putative mitochondrial ribosomal protein L17 OS=Sc... [more]
A0A183NS794.339e-3136.84Uncharacterized protein OS=Schistosoma mattheei OX... [more]
A0A430QRM85.964e-3135.87Large subunit ribosomal protein L17 OS=Schistosoma... [more]
A0A183K6676.423e-3135.87Uncharacterized protein OS=Schistosoma curassoni O... [more]
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BLAST of SMED30001783 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl176.544e-1530.72mitochondrial ribosomal protein L17 [Source:ZFIN;A... [more]
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BLAST of SMED30001783 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001783 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001783 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO322089.774e-829.82Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30001783 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl171.011e-1638.52mitochondrial ribosomal protein L17 [Source:NCBI g... [more]
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BLAST of SMED30001783 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000040740.16.476e-148100.00SMESG000040740.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001783 ID=SMED30001783|Name=SMED30001783|organism=Schmidtea mediterranea sexual|type=transcript|length=767bp
TAAGATAACTACAAAATTTATTTATTTGTTTTCCACTCTTCTTTGGCAGC
TGAAACGAGAATATTAGTCAAAAATTGAGACTTGTTTTGTGATTTCAATG
CTTGATATCTCGTTTTAATAGGGGGGAAGGAATTTCCTTTCAATTCGAGA
ACTGCGTTGTGATCGGGAAACTTAATTTTTGTTTCGGATCTTGGAATCGG
CAATTTAAATAATCGAGTAAATGATAACGTTGAATGTCTGTATCGTGGAA
CTAAATCTTCGAACATCTTTTTAACTAATTTAGATTCTTGAAGCCAAAAT
TCCATCAATTCTATATTAAATGGACAGTTTGGGCCTTTAATTATGGCTTC
TTGTATTAGTCTTTCAGCATACTTTCTTGTCTCAAGAGCGTGTTTATGTT
TTAATTCTATACGTTCTTCATCAATTAAAGATGTCACACATCTTCTCATT
AAAAACATTTGTCCAATATAGCCACTTCCAACTCCTTCACCATAATATAT
TTTATTTTCTTTATCACCTATATTGAACTTTAATTGATATGCAGCGTTAC
CAATTTTAGCAGACGAGCAATTGCGAATAATACCCTTTCGGAAAGTACAT
AACCTTTTAAACATCTAGATATCAAAATTATATCGATGAAGAAAAATTAA
TATTAGTTAAAAATCTTTAAGTGAATAAAAATTGTAAAATATACAATATA
AAATGTAATTATATAACATGTCATTTCAAATTTAGCAGAAAATTTATTAA
TTCAAAGAAAATTCCAG
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protein sequence of SMED30001783-orf-1

>SMED30001783-orf-1 ID=SMED30001783-orf-1|Name=SMED30001783-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=199bp
MFKRLCTFRKGIIRNCSSAKIGNAAYQLKFNIGDKENKIYYGEGVGSGYI
GQMFLMRRCVTSLIDEERIELKHKHALETRKYAERLIQEAIIKGPNCPFN
IELMEFWLQESKLVKKMFEDLVPRYRHSTLSFTRLFKLPIPRSETKIKFP
DHNAVLELKGNSFPPIKTRYQALKSQNKSQFLTNILVSAAKEEWKTNK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR036373Ribosomal_L17_sf
IPR000456Ribosomal_L17
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
GO:0005840ribosome
Vocabulary: biological process
TermDefinition
GO:0006412translation
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036373Ribosomal protein L17 superfamilyGENE3DG3DSA:3.90.1030.10coord: 51..161
e-value: 3.4E-20
score: 74.2
IPR036373Ribosomal protein L17 superfamilySUPERFAMILYSSF64263Prokaryotic ribosomal protein L17coord: 55..165
IPR000456Ribosomal protein L17PFAMPF01196Ribosomal_L17coord: 55..140
e-value: 8.1E-12
score: 45.9
IPR000456Ribosomal protein L17PANTHERPTHR14413RIBOSOMAL PROTEIN L17coord: 54..174
NoneNo IPR availablePANTHERPTHR14413:SF1639S RIBOSOMAL PROTEIN L17, MITOCHONDRIALcoord: 54..174