G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30001754
Length (bp)1510
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30001754) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30001754) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30001754) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30001754) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30001754

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30001754h1SMcG0016771 SmedASXL_000604SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30001754h1SMcG0016771 SmedASXL_000883SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30001754h1SMcG0016771 dd_Smed_v4_25618_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30001754h1SMcG0016771 dd_Smed_v4_25618_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30001754h1SMcG0016771 dd_Smed_v4_25618_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30001754h1SMcG0016771 dd_Smed_v4_25618_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30001754h1SMcG0016771 dd_Smed_v6_25618_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Match: ckr-2 (CholecystoKinin Receptor homolog [Source:UniProtKB/TrEMBL;Acc:G4SNP8])

HSP 1 Score: 52.7582 bits (125), Expect = 3.777e-7
Identity = 31/89 (34.83%), Postives = 52/89 (58.43%), Query Frame = 3 
Query:  441 GNSLV-IFVFQRNAQNSSSNYFIVVLGAIDLLICTFCIPTTL---VIEIWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLIC 695
            GNS+V I + ++ A  S +N +++ L A D+++   C+P TL   V+  W F  Y  +CK+L++ +  +V  S   L +IAF+R   IC
Sbjct:   35 GNSVVLIVIIKQRAMRSITNIYLMNLAASDMMLSVVCMPPTLVSMVMNCWMFGNY--MCKILAYLQPVVVTASAYTLAVIAFERYFAIC 121
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Match: ckr-2 (CholecystoKinin Receptor homolog [Source:UniProtKB/TrEMBL;Acc:G4SNP8])

HSP 1 Score: 53.1434 bits (126), Expect = 3.983e-7
Identity = 31/89 (34.83%), Postives = 52/89 (58.43%), Query Frame = 3 
Query:  441 GNSLV-IFVFQRNAQNSSSNYFIVVLGAIDLLICTFCIPTTL---VIEIWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLIC 695
            GNS+V I + ++ A  S +N +++ L A D+++   C+P TL   V+  W F  Y  +CK+L++ +  +V  S   L +IAF+R   IC
Sbjct:   77 GNSVVLIVIIKQRAMRSITNIYLMNLAASDMMLSVVCMPPTLVSMVMNCWMFGNY--MCKILAYLQPVVVTASAYTLAVIAFERYFAIC 163
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Match: ckr-2 (CholecystoKinin Receptor homolog [Source:UniProtKB/TrEMBL;Acc:G4SNP8])

HSP 1 Score: 52.373 bits (124), Expect = 6.179e-7
Identity = 31/89 (34.83%), Postives = 52/89 (58.43%), Query Frame = 3 
Query:  441 GNSLV-IFVFQRNAQNSSSNYFIVVLGAIDLLICTFCIPTTL---VIEIWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLIC 695
            GNS+V I + ++ A  S +N +++ L A D+++   C+P TL   V+  W F  Y  +CK+L++ +  +V  S   L +IAF+R   IC
Sbjct:   77 GNSVVLIVIIKQRAMRSITNIYLMNLAASDMMLSVVCMPPTLVSMVMNCWMFGNY--MCKILAYLQPVVVTASAYTLAVIAFERYFAIC 163
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Match: npr-3 (NeuroPeptide Receptor family [Source:UniProtKB/TrEMBL;Acc:P90745])

HSP 1 Score: 51.6026 bits (122), Expect = 7.508e-7
Identity = 35/96 (36.46%), Postives = 52/96 (54.17%), Query Frame = 3 
Query:  441 GNSLVIFVFQRNAQN-SSSNYFIVVLGAIDLLICTFCIPTTLVI---EIWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLICFVPSKQI 716
            GN+LV++V   N  + S    F+  L   DLL+C F +P T +     +W FP   I CKL+  F+   +  S   L +IA DRC+LI   P+++I
Sbjct:   42 GNTLVLYVLTFNQVSLSVRTVFVGCLAGSDLLMCLFSLPITAISIFSRVWVFPA--IFCKLIGVFQGGTIFVSSFTLTVIALDRCVLI-LRPNQEI 134
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Match: ckr-2 (CholecystoKinin Receptor homolog [Source:UniProtKB/TrEMBL;Acc:G4SNP8])

HSP 1 Score: 51.6026 bits (122), Expect = 8.164e-7
Identity = 31/89 (34.83%), Postives = 52/89 (58.43%), Query Frame = 3 
Query:  441 GNSLV-IFVFQRNAQNSSSNYFIVVLGAIDLLICTFCIPTTL---VIEIWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLIC 695
            GNS+V I + ++ A  S +N +++ L A D+++   C+P TL   V+  W F  Y  +CK+L++ +  +V  S   L +IAF+R   IC
Sbjct:   35 GNSVVLIVIIKQRAMRSITNIYLMNLAASDMMLSVVCMPPTLVSMVMNCWMFGNY--MCKILAYLQPVVVTASAYTLAVIAFERYFAIC 121
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Match: npy2r (neuropeptide Y receptor Y2 [Source:ZFIN;Acc:ZDB-GENE-040924-8])

HSP 1 Score: 51.6026 bits (122), Expect = 1.281e-6
Identity = 34/84 (40.48%), Postives = 50/84 (59.52%), Query Frame = 3 
Query:  441 GNSLVIFV-FQRNAQNSSSNYFIVVLGAIDLLICTFCIPTTLVIEI---WRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDR 680
            GNSLVI+V ++    ++ +NYFIV L   DLL+ T C+P TL+  +   W+F    ++C LL + +   V  S I L +IA DR
Sbjct:   72 GNSLVIYVVYKFRNLHTVTNYFIVNLAVADLLVNTLCLPFTLMYTLYGEWKFG--QVMCYLLPYAQGLAVHVSTITLNVIALDR 153
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Match: npy2r (neuropeptide Y receptor Y2 [Source:ZFIN;Acc:ZDB-GENE-040924-8])

HSP 1 Score: 51.6026 bits (122), Expect = 1.362e-6
Identity = 34/84 (40.48%), Postives = 50/84 (59.52%), Query Frame = 3 
Query:  441 GNSLVIFV-FQRNAQNSSSNYFIVVLGAIDLLICTFCIPTTLVIEI---WRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDR 680
            GNSLVI+V ++    ++ +NYFIV L   DLL+ T C+P TL+  +   W+F    ++C LL + +   V  S I L +IA DR
Sbjct:   72 GNSLVIYVVYKFRNLHTVTNYFIVNLAVADLLVNTLCLPFTLMYTLYGEWKFG--QVMCYLLPYAQGLAVHVSTITLNVIALDR 153
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Match: npffr1l2 (neuropeptide FF receptor 1 like 2 [Source:ZFIN;Acc:ZDB-GENE-060526-110])

HSP 1 Score: 49.6766 bits (117), Expect = 6.380e-6
Identity = 35/92 (38.04%), Postives = 48/92 (52.17%), Query Frame = 3 
Query:  441 GNSLV-IFVFQRNAQNSSSNYFIVVLGAIDLLICTFCIPTTLVIE-IWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFD--RCLLICFVP 704
            GN LV + V +     + +N FI+ L   DLL+  FCIPTTLV   I  +P  + VCK+    +   V  S   L+ IA D  RC++  F P
Sbjct:   69 GNGLVCLIVLENRRMRTVTNLFILNLAVSDLLVGVFCIPTTLVDNLITGWPFTNTVCKMSGLVQGMSVSASVFTLVAIAVDRFRCIVYPFQP 160
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Match: npffr1l2 (neuropeptide FF receptor 1 like 2 [Source:ZFIN;Acc:ZDB-GENE-060526-110])

HSP 1 Score: 49.6766 bits (117), Expect = 6.689e-6
Identity = 35/92 (38.04%), Postives = 48/92 (52.17%), Query Frame = 3 
Query:  441 GNSLV-IFVFQRNAQNSSSNYFIVVLGAIDLLICTFCIPTTLVIE-IWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFD--RCLLICFVP 704
            GN LV + V +     + +N FI+ L   DLL+  FCIPTTLV   I  +P  + VCK+    +   V  S   L+ IA D  RC++  F P
Sbjct:   65 GNGLVCLIVLENRRMRTVTNLFILNLAVSDLLVGVFCIPTTLVDNLITGWPFTNTVCKMSGLVQGMSVSASVFTLVAIAVDRFRCIVYPFQP 156
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Match: NPY2R (neuropeptide Y receptor Y2 [Source:NCBI gene;Acc:100498351])

HSP 1 Score: 55.0694 bits (131), Expect = 1.781e-7
Identity = 35/100 (35.00%), Postives = 58/100 (58.00%), Query Frame = 3 
Query:  441 GNSLVIFVFQR-NAQNSSSNYFIVVLGAIDLLICTFCIPTTLV---IEIWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLICFVPSKQIKVKL 728
            GNSLVI+V  +     + +N+FIV L   DL++ T C+P TLV   ++ W+F T  ++C L+++ +   V  S + L++IA DR   I +    +I  K+
Sbjct:   90 GNSLVIYVVIKFKTMRTVTNFFIVNLAVSDLMVNTLCLPFTLVYTLLDDWKFGT--VLCHLVTYSQGLAVQVSTVTLMVIALDRHRCIVYHLESKISTKI 187
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Match: taf1b (TATA-box binding protein associated factor, RNA polymerase I, B [Source:Xenbase;Acc:XB-GENE-992275])

HSP 1 Score: 50.0618 bits (118), Expect = 5.314e-6
Identity = 34/101 (33.66%), Postives = 57/101 (56.44%), Query Frame = 3 
Query:  441 GNSLVIF-VFQRNAQNSSSNYFIVVLGAIDLLICTFCIPTTLV---IEIWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLICFVPSKQIKVKLA 731
            GNSLVI+ + +     + +N+FI  L   DL++ +FC+P TLV   ++ W+F +  ++C L  + +   V  S + LI+IA DR   I F  + +I   L+
Sbjct:   56 GNSLVIYMIVKYKNMRTVTNFFIANLAIADLMVDSFCLPFTLVYTLMDEWKFGS--VLCHLFPYAQAMSVNVSTLTLIVIALDRYWCIVFHLNSRISKNLS 154
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Match: tcf12 (transcription factor 12 (HTF4, helix-loop-helix transcription factors 4) [Source:Xenbase;Acc:XB-GENE-5799694])

HSP 1 Score: 50.0618 bits (118), Expect = 7.030e-6
Identity = 33/87 (37.93%), Postives = 48/87 (55.17%), Query Frame = 3 
Query:  441 GNSLVIFVFQRNAQ-NSSSNYFIVVLGAIDLLICTFCIPTTLVIEIWR-FPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLIC 695
            GNSLVI V  RN +  + +N F++ L   DL++C FC+P TL+  + R F     VCK  ++F    V  S   L+ I+ +R   IC
Sbjct:   58 GNSLVIAVLIRNKRMRTVTNIFLLSLAVSDLMLCLFCMPFTLIPNLLRDFIFGSAVCKAATYFMGISVSVSTFNLVAISLERYSAIC 144
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Match: tcf12 (transcription factor 12 (HTF4, helix-loop-helix transcription factors 4) [Source:Xenbase;Acc:XB-GENE-5799694])

HSP 1 Score: 49.6766 bits (117), Expect = 7.606e-6
Identity = 33/87 (37.93%), Postives = 48/87 (55.17%), Query Frame = 3 
Query:  441 GNSLVIFVFQRNAQ-NSSSNYFIVVLGAIDLLICTFCIPTTLVIEIWR-FPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLIC 695
            GNSLVI V  RN +  + +N F++ L   DL++C FC+P TL+  + R F     VCK  ++F    V  S   L+ I+ +R   IC
Sbjct:   48 GNSLVIAVLIRNKRMRTVTNIFLLSLAVSDLMLCLFCMPFTLIPNLLRDFIFGSAVCKAATYFMGISVSVSTFNLVAISLERYSAIC 134
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Match: tcf12 (transcription factor 12 (HTF4, helix-loop-helix transcription factors 4) [Source:Xenbase;Acc:XB-GENE-5799694])

HSP 1 Score: 49.6766 bits (117), Expect = 7.859e-6
Identity = 33/87 (37.93%), Postives = 48/87 (55.17%), Query Frame = 3 
Query:  441 GNSLVIFVFQRNAQ-NSSSNYFIVVLGAIDLLICTFCIPTTLVIEIWR-FPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLIC 695
            GNSLVI V  RN +  + +N F++ L   DL++C FC+P TL+  + R F     VCK  ++F    V  S   L+ I+ +R   IC
Sbjct:   47 GNSLVIAVLIRNKRMRTVTNIFLLSLAVSDLMLCLFCMPFTLIPNLLRDFIFGSAVCKAATYFMGISVSVSTFNLVAISLERYSAIC 133
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183WA62 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS9015 PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 4.156e-11
Identity = 41/96 (42.71%), Postives = 60/96 (62.50%), Query Frame = 3 
Query:  441 GNSLVIFVFQRN-AQNSSSNYFIVVLGAIDLLICTFCIPTTLVIEIWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLICFVPSKQIKVK 725
            GNSLV++ F++  +Q   S  FI++L   DL IC   IP TL ++IW   T D +C++    + F+V  S  +L+LIA +R LLICF+P  Q+K K
Sbjct:   58 GNSLVVWAFRKQVSQGIVSGLFILLLACNDLFICLIVIPCTLYLDIWTGDTTDWICRIHLSMKGFVVPISACILMLIAMERFLLICFIPGIQMKRK 153
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A448X8C9 (G_PROTEIN_RECEP_F1_2 domain-containing protein (Fragment) OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS24171 PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 5.941e-11
Identity = 38/96 (39.58%), Postives = 60/96 (62.50%), Query Frame = 3 
Query:  441 GNSLVIFVFQRNAQNSS-SNYFIVVLGAIDLLICTFCIPTTLVIEIWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLICFVPSKQIKVK 725
            GN LVI+ F R A+ +  S+  I++LG  DL+IC   IP TL +++W      ++C+L    + F++ FS  LL+LIA +R LLICF+P   ++ +
Sbjct:   61 GNLLVIWAFSRQARRAVVSSLLIILLGITDLVICLVDIPVTLYLKVWFREATVVLCRLHGGLKGFIIPFSSGLLVLIALERFLLICFMPGTPLRPR 156
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A5K4F7Z1 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 9.618e-11
Identity = 41/94 (43.62%), Postives = 58/94 (61.70%), Query Frame = 3 
Query:  441 GNSLVIFVFQRN-AQNSSSNYFIVVLGAIDLLICTFCIPTTLVIEIWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLICFVPSKQIK 719
            GNSLVI+ F++   Q   S  FI++L   DL IC   IP TL ++IW   T D +C++    + F+V  S  +L+LIA +R LLICF+P  Q+K
Sbjct:   58 GNSLVIWAFRKQVKQGIISGLFILLLACNDLFICLIVIPCTLYLDIWTGDTTDWICRIHLTMKGFVVPISACILMLIAMERFLLICFIPGIQMK 151
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A074ZDK4 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_08498 PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 3.186e-10
Identity = 37/94 (39.36%), Postives = 60/94 (63.83%), Query Frame = 3 
Query:  441 GNSLVIFVFQRNA-QNSSSNYFIVVLGAIDLLICTFCIPTTLVIEIWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLICFVPSKQIK 719
            GN LV++ F+ +A Q   ++  I+ L  +DLLIC   IP+TL +++W   T DI+C++   F+ F+V  S  +L+ IA +R  LICF+P  ++K
Sbjct:  334 GNILVVWAFRCHASQGIVTSMLILTLACLDLLICVVGIPSTLYLDVWSGETTDIICRIHMAFKGFIVPVSACVLVFIALERFFLICFIPGFELK 427
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A095CBI3 (Coiled-coil domain-containing protein 147 OS=Schistosoma haematobium OX=6185 GN=MS3_08287 PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 3.398e-10
Identity = 39/94 (41.49%), Postives = 56/94 (59.57%), Query Frame = 3 
Query:  441 GNSLVIFVFQRNA-QNSSSNYFIVVLGAIDLLICTFCIPTTLVIEIWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLICFVPSKQIK 719
            GNSLV++ F +   Q   S  FI++L   DL IC   IP TL ++IW   T D +C+     + F+V  S  +L+LIA +R LLICF+P  Q++
Sbjct:   38 GNSLVVWAFTKQVKQGIISGLFILLLACNDLFICLIVIPCTLYLDIWTGDTTDWICRAHLTMKGFVVPVSACILMLIAMERFLLICFIPGIQMQ 131
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Match: hcrtr2 (hypocretin receptor 2 [Source:NCBI gene;Acc:103037678])

HSP 1 Score: 50.447 bits (119), Expect = 2.935e-6
Identity = 33/89 (37.08%), Postives = 50/89 (56.18%), Query Frame = 3 
Query:  441 GNSLVIFVFQRNAQ-NSSSNYFIVVLGAIDLLICTFCIPTTLVIEI---WRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLIC 695
            GN+LV F   +N    + +NYFIV L   D+L+   C+P +LV++I   W     D++C++L +F+   V  S + L  IA DR   IC
Sbjct:   70 GNALVCFAVWKNHHMRTVTNYFIVNLSFADILVTITCLPASLVVDITETWFLG--DMLCRILPYFQTISVSVSVLTLSCIAQDRWYAIC 156
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000062718.1 (SMESG000062718.1)

HSP 1 Score: 108.997 bits (271), Expect = 3.528e-25
Identity = 52/102 (50.98%), Postives = 72/102 (70.59%), Query Frame = 3 
Query:  441 GNSLVIFVFQRNAQ-------NSSSNYFIVVLGAIDLLICTFCIPTTLVIEIWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLICFVPSKQIKVK 725
            GN ++I V  +  Q       N SS   ++ L  +DLL+C   +PT+L++EIWR PTYDI CK++  FRCFL+IFSP+LL+LIA DR L+ICFVPSK++ +K
Sbjct:   60 GNCILILVLIKQIQKRTGHSTNLSSIILVLSLALVDLLVCIVSVPTSLIVEIWRLPTYDIACKIVMAFRCFLLIFSPMLLVLIALDRFLIICFVPSKKMSLK 161
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000050757.1 (SMESG000050757.1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.582e-16
Identity = 43/97 (44.33%), Postives = 61/97 (62.89%), Query Frame = 3 
Query:  441 GNSLVIFVFQRNAQNSSSNYFIVVLGAIDLLICTFCIPTTLVIEIWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLICFVPSKQIKVKLA 731
            GN  VI VF ++ + + SN FI  L  ID++IC   IP TL + +W+  + DIVCKL    + F V  S ++L++IA DR L+ICF+PS  I+ K A
Sbjct:   47 GNYFVIVVFSKHIKQAVSNLFITFLAIIDMIICVIDIPMTLYLVVWQKNSVDIVCKLHISLKAFCVPVSALILVMIAIDRFLIICFIPSILIRKKSA 143
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000064085.1 (SMESG000064085.1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.156e-9
Identity = 36/94 (38.30%), Postives = 56/94 (59.57%), Query Frame = 3 
Query:  441 GNSLVIFVFQRNAQNSSSN--YFIVVLGAIDLLICTFCIPTTLVIEIWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLICFVPSKQI 716
            GN LV+ V+ RN +   +N  +FI++L A DL +C   +P T+ +E+  F   +I CK   +     ++FS +L+++IAFDR L IC  P K I
Sbjct:   30 GNFLVLLVY-RNKRRHENNDIFFIIILAASDLAVCIILVPGTIYVELKAFEISEIACKSYYFLNTSNLVFSTLLILVIAFDRYLSICH-PLKSI 121
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000066072.1 (SMESG000066072.1)

HSP 1 Score: 53.1434 bits (126), Expect = 2.433e-7
Identity = 39/101 (38.61%), Postives = 59/101 (58.42%), Query Frame = 3 
Query:  441 GNSLVIFVFQRN-AQNSSSNYFIVVLGAIDLLICTFCIPTT---LVIEIWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLICFVPSKQIKVKLA 731
            GN+LVIFV  RN A  + +N+FI  L A D+L+C   +P T   L +E W  P  ++VCKLL       V  S +  ++I+ DR  +I + P K +++KL+
Sbjct:   44 GNALVIFVILRNKAMYTVTNFFITNLAASDILMCIVAVPFTPIALYLENWTLP--ELVCKLLPMTMAISVYVSTLTSLVISIDRYFVIVY-PFK-VRMKLS 140
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000064403.1 (SMESG000064403.1)

HSP 1 Score: 51.9878 bits (123), Expect = 5.832e-7
Identity = 32/86 (37.21%), Postives = 44/86 (51.16%), Query Frame = 3 
Query:  441 GNSLVIFVFQRNAQNSSSNYFIVVLGAIDLLICTFCIPTTLVIEIWRFP-TYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLIC 695
            GN LVI V+ R     ++  FI +L   D   C   IP T+ IE W +  +   +CKL  +F    + FS  L+ LIA DR + IC
Sbjct:   39 GNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICIC 124
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ckr-23.777e-734.83CholecystoKinin Receptor homolog [Source:UniProtK... [more]
ckr-23.983e-734.83CholecystoKinin Receptor homolog [Source:UniProtK... [more]
ckr-26.179e-734.83CholecystoKinin Receptor homolog [Source:UniProtK... [more]
npr-37.508e-736.46NeuroPeptide Receptor family [Source:UniProtKB/Tr... [more]
ckr-28.164e-734.83CholecystoKinin Receptor homolog [Source:UniProtK... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
npy2r1.281e-640.48neuropeptide Y receptor Y2 [Source:ZFIN;Acc:ZDB-GE... [more]
npy2r1.362e-640.48neuropeptide Y receptor Y2 [Source:ZFIN;Acc:ZDB-GE... [more]
npffr1l26.380e-638.04neuropeptide FF receptor 1 like 2 [Source:ZFIN;Acc... [more]
npffr1l26.689e-638.04neuropeptide FF receptor 1 like 2 [Source:ZFIN;Acc... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
NPY2R1.781e-735.00neuropeptide Y receptor Y2 [Source:NCBI gene;Acc:1... [more]
taf1b5.314e-633.66TATA-box binding protein associated factor, RNA po... [more]
tcf127.030e-637.93transcription factor 12 (HTF4, helix-loop-helix tr... [more]
tcf127.606e-637.93transcription factor 12 (HTF4, helix-loop-helix tr... [more]
tcf127.859e-637.93transcription factor 12 (HTF4, helix-loop-helix tr... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183WA624.156e-1142.71G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A448X8C95.941e-1139.58G_PROTEIN_RECEP_F1_2 domain-containing protein (Fr... [more]
A0A5K4F7Z19.618e-1143.62Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A074ZDK43.186e-1039.36Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A095CBI33.398e-1041.49Coiled-coil domain-containing protein 147 OS=Schis... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
hcrtr22.935e-637.08hypocretin receptor 2 [Source:NCBI gene;Acc:103037... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000062718.13.528e-2550.98SMESG000062718.1[more]
SMESG000050757.11.582e-1644.33SMESG000050757.1[more]
SMESG000064085.11.156e-938.30SMESG000064085.1[more]
SMESG000066072.12.433e-738.61SMESG000066072.1[more]
SMESG000064403.15.832e-737.21SMESG000064403.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001754 ID=SMED30001754|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1510bp
ATTCCATACGGATCAGACATTGAAATAGCAAAAAATAATAAAAGATATAA
TAAAAGCAAAATTATTTATATCGAAATTATAAATGAAGTGAAAATTTTTC
AATGGTCAAAAATAAACCAATAAAAAAATAAAACGACAAGTAGCTCGTTA
TAAGAATGCAATACAATTTGAAGTAATAGAAAAAGAGAAAATTATCTAAT
ATTCGGTGAGAAGATTTTATGCAAATTCGTCAAGCAAATTATTTGAAAAA
CTATAGAAAATATTTATGAATTATGGATGGAAATTCGTATTTGTTAAAAA
TAATGGAAGAAAAAAATCCTTCAAAAATAAACATTCAGGATATTATCAAA
TTCATGGACAATAAATATCATAACAAGTTTGTGTTGGTTGATATTTTATT
AACTATTATTTTGATCATTATCTCTGTGCTCGGAATAATCGGAAACAGTT
TGGTCATTTTCGTTTTCCAAAGGAATGCTCAAAATTCATCCTCAAACTAT
TTTATTGTCGTTTTGGGTGCAATTGACTTACTGATTTGCACTTTTTGTAT
TCCAACAACACTGGTAATTGAGATTTGGAGATTTCCAACTTATGATATTG
TTTGCAAATTGTTGAGTTGGTTTCGTTGTTTTTTGGTCATCTTTTCTCCC
ATACTTTTGATTCTGATAGCTTTCGATCGATGTTTGTTGATATGTTTTGT
TCCAAGTAAACAAATAAAAGTGAAACTAGCACCGGTTTTGTTGATAATTA
TCATTATTATCAGTACCGGCCTCTCGATACCATCGGGTCTGATATTAGGA
ACCTTTGTCAGAGTCAATCAACCGAATGTCACAGAATCTGGTTTAAATGA
AACTTTAGAAACGCGACTTATTCGAAATCTTAATGCACATGTTTCATTGA
CAAGTTCGTGTCATGTTGATGAAAGATTCATAAATGCAAAACAGTTTAAC
ATCTATCGCTATTTTGTTATGATTCTATTCTTTTCCATTCTTTCCAGCTT
GATTTTATTATATGTCTCGATTTTCACTTTTATCATCATTCAACATAGCC
GATGGATGAAGCATAACCAAAACTATAATTTTAATGTAAAGTCAAGCTTA
ATAAACCAGAAAAAGATTCCAATGAACCAAAACTAACAGTGAACTCCAAA
ACGGGTAATGTGAATCTGACCTCGACTGAAATGTCCTCAATTAACAAATC
CAAATCAACAAACAATGTCAAAATATCCTATGTTAAAGAGGTTTGCAGTA
CTCTAATTAGCTCGCCAATAAAGCGAAAATCAAAGCCGAGAAATGATTCA
ATGAAAAGTGAAACATTTTCTACAATGCAATCTATAGTCCGGCCAAAATC
AACAGATAGAAAGCCATCTCGAAGTATTAAAACATTGAAAACTTTGCTTC
TTGTGTCTTTGTCGTTTATTTCTTCATTTGTTCCATACTTATTAGTAAGT
ACAAGACTTATATTTTCCAAAAACGACTTATCTAATAAAGAGATCTTAGT
TAAATCGTTG
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protein sequence of SMED30001754-orf-1

>SMED30001754-orf-1 ID=SMED30001754-orf-1|Name=SMED30001754-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=288bp
MDGNSYLLKIMEEKNPSKINIQDIIKFMDNKYHNKFVLVDILLTIILIII
SVLGIIGNSLVIFVFQRNAQNSSSNYFIVVLGAIDLLICTFCIPTTLVIE
IWRFPTYDIVCKLLSWFRCFLVIFSPILLILIAFDRCLLICFVPSKQIKV
KLAPVLLIIIIIISTGLSIPSGLILGTFVRVNQPNVTESGLNETLETRLI
RNLNAHVSLTSSCHVDERFINAKQFNIYRYFVMILFFSILSSLILLYVSI
FTFIIIQHSRWMKHNQNYNFNVKSSLINQKKIPMNQN*
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protein sequence of SMED30001754-orf-2

>SMED30001754-orf-2 ID=SMED30001754-orf-2|Name=SMED30001754-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=110bp
MSSINKSKSTNNVKISYVKEVCSTLISSPIKRKSKPRNDSMKSETFSTMQ
SIVRPKSTDRKPSRSIKTLKTLLLVSLSFISSFVPYLLVSTRLIFSKNDL
SNKEILVKSL
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
PLANA:0002075pigment cell
PLANA:0003116parenchymal cell
PLANA:0003117parapharyngeal cell
Vocabulary: molecular function
TermDefinition
GO:0004871signal transducer activity
GO:0004930G-protein coupled receptor activity
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
GO:0007165signal transduction
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 118..140
score: 26.35
coord: 74..95
score: 28.4
coord: 228..251
score: 18.46
coord: 42..66
score: 27.58
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 57..267
e-value: 9.3E-13
score: 48.1
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 28..284
e-value: 2.1E-29
score: 104.5
NoneNo IPR availablePANTHERPTHR24238G-PROTEIN COUPLED RECEPTORcoord: 23..272
NoneNo IPR availableCDDcd152037tmA_NPYR-likecoord: 57..170
e-value: 1.05584E-15
score: 73.4075
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 1..267
NoneNo IPR availableTMHMMTMhelixcoord: 114..134
NoneNo IPR availableTMHMMTMhelixcoord: 77..99
NoneNo IPR availableTMHMMTMhelixcoord: 234..256
NoneNo IPR availableTMHMMTMhelixcoord: 42..64
NoneNo IPR availableTMHMMTMhelixcoord: 155..177
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 57..288
score: 18.546