SMG5 nonsense mediated mRNA decay factor

Overview
NameSMG5 nonsense mediated mRNA decay factor
Smed IDSMED30001753
Length (bp)3299
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMG5 nonsense mediated mRNA decay factor (SMED30001753) t-SNE clustered cells

Violin plots show distribution of expression levels for SMG5 nonsense mediated mRNA decay factor (SMED30001753) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMG5 nonsense mediated mRNA decay factor (SMED30001753) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMG5 nonsense mediated mRNA decay factor (SMED30001753) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30001753

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30001753SMESG000046002.1 SmedASXL_016809SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30001753SMESG000046002.1 SmedASXL_016809SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Human
Match: SMG5 (SMG5 nonsense mediated mRNA decay factor [Source:HGNC Symbol;Acc:HGNC:24644])

HSP 1 Score: 152.91 bits (385), Expect = 6.712e-37
Identity = 143/534 (26.78%), Postives = 244/534 (45.69%), Query Frame = 1 
Query:  130 KTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFV-PCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDMNHYDIKQLAAMSDDVL---NILSFVLGFDRKLVSNLSSTDNGVRPST---LNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLKFDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLLFHRNENKEEISVIKETCDEISSSKSTVAISPDHSVVKSRKKRVGLSKRLVRHRHDTDEASIEEDLSEDDQNLSENDNILWDDQISDSSDSNVGDEGGFGFSDVEDETDFESNE 1710
            K L R V E + +L  +   K A + +   E  +LR KL+E   +L+FL P+++G+KAE+ +W+ ++YE I++ K   K+    I+S+S +  A++ H++  IG+YQ L+L +Q+ ++L  + C D+T+   P +  ++    S +   WA       L+Y+GDL RY++++   ++ +LA R+Y Q LS+ P +G  +NQL TL    YY + A Y YL+      SF  A  NL  L+++  K           +KK  +     G K  R K      +N  Y  + L   +     +L ++   VL   N+  F L     L  +L+S D     S    L   ++ Q++ I   C++ +              +  S ++  A +F L L+ H+ N   +R++ EL      +   F  +   E  S      +E    +            KSRK       R  RH     + S   DLSE  ++ S +D+    +     SD ++  EGG    D E +++  S E
Sbjct:   21 KRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKH----IHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQ-------LKKCETRKLSPGKK--RCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNL--SLASEDEEEYESGYAFLPDLLIFQMVIICLMCVHSL-------------ERAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVP-AFQSDGTDEPESKEPVEKEEEPDPEPPPVTPQVGEGRKSRKFSRLSCLRRRRHPPKVGDDS---DLSEGFESDSSHDSARASEGSDSGSDKSL--EGGGTAFDAETDSEMNSQE 520

HSP 2 Score: 71.2478 bits (173), Expect = 1.039e-11
Identity = 37/117 (31.62%), Postives = 61/117 (52.14%), Query Frame = 1 
Query: 2590 KEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIE 2940
            + ++MR+MA+LRL+ E+S    S               + P   +  + YL+PD + +C+ L  I+ L    R+I +IP+ VI  LD +K    GAR  I+Y E E +  N +I+ +
Sbjct:  821 RNRLMRDMAQLRLQLEVSQLEGS--------------LQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQ 923
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Human
Match: SMG6 (SMG6 nonsense mediated mRNA decay factor [Source:HGNC Symbol;Acc:HGNC:17809])

HSP 1 Score: 69.3218 bits (168), Expect = 4.056e-11
Identity = 74/298 (24.83%), Postives = 130/298 (43.62%), Query Frame = 1 
Query:   97 IRIRQNASVKEKTLI---RVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLN 975
            +R+  N  ++   L+   R+  E L+K+ QL       E+L  +E             R I L+ +EF   Q  +  +WK+ FY+ I  F R+L   PN+ N + +     +    G+  ++  L+ +LQ  ++   E   D+ + +   S    K +      +A    Q  ++  GD+ RY+     T +   A  +Y +   + P  G  YNQLA L   +   L+A Y+Y+++   SN    AKE+L+ LFE   ++       E M KK       S  +  + K S  +++ 
Sbjct:  585 LRVADNQELQLSNLLSRDRISPEGLEKMAQLRA-----ELLQLYE-------------RCILLD-IEFSDNQNVDQILWKNAFYQVIEKF-RQLVKDPNVENPEQIRNRLLELLDEGS-DFFDSLLQKLQVTYKFKLE---DYMDGLAIRSKPLRKTVK-----YALISAQRCMICQGDIARYREQASDTANYGKARSWYLKAQHIAPKNGRPYNQLALLAVYTRRKLDAVYYYMRSLAASNPILTAKESLMSLFEETKRKA------EQMEKKQHEEFDLSPDQWRKGKKSTFRHVG 847
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Human
Match: SMG7 (SMG7 nonsense mediated mRNA decay factor [Source:HGNC Symbol;Acc:HGNC:16792])

HSP 1 Score: 59.3066 bits (142), Expect = 5.051e-8
Identity = 56/223 (25.11%), Postives = 91/223 (40.81%), Query Frame = 1 
Query:  208 LSTFEIQALRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPC------------VSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENL 834
            L   E+   R  L++   +++  + LE+   +K E  +W   F   I   + + KN+ N   ++S +       +  A G+Y           +LL E+C  F   +PC              T     + P+  S ++ C QH L+++GD+ RY+      N    A  YY     L PS G  YNQLA L +     L   ++Y ++      F  A  NL
Sbjct:   24 LGPAEVWTSRQALQDLYQKMLVTD-LEYALDKKVEQDLWNHAFKNQITTLQGQAKNRAN--PNRSEVQANLSLFLEAASGFYT----------QLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYIC-QHCLVHLGDIARYR------NQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNL 226
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Human
Match: SMG7 (SMG7 nonsense mediated mRNA decay factor [Source:HGNC Symbol;Acc:HGNC:16792])

HSP 1 Score: 58.9214 bits (141), Expect = 6.388e-8
Identity = 56/223 (25.11%), Postives = 91/223 (40.81%), Query Frame = 1 
Query:  208 LSTFEIQALRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPC------------VSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENL 834
            L   E+   R  L++   +++  + LE+   +K E  +W   F   I   + + KN+ N   ++S +       +  A G+Y           +LL E+C  F   +PC              T     + P+  S ++ C QH L+++GD+ RY+      N    A  YY     L PS G  YNQLA L +     L   ++Y ++      F  A  NL
Sbjct:   24 LGPAEVWTSRQALQDLYQKMLVTD-LEYALDKKVEQDLWNHAFKNQITTLQGQAKNRAN--PNRSEVQANLSLFLEAASGFYT----------QLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYIC-QHCLVHLGDIARYR------NQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNL 226
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Human
Match: SMG7 (SMG7 nonsense mediated mRNA decay factor [Source:HGNC Symbol;Acc:HGNC:16792])

HSP 1 Score: 58.9214 bits (141), Expect = 6.731e-8
Identity = 56/223 (25.11%), Postives = 91/223 (40.81%), Query Frame = 1 
Query:  208 LSTFEIQALRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPC------------VSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENL 834
            L   E+   R  L++   +++  + LE+   +K E  +W   F   I   + + KN+ N   ++S +       +  A G+Y           +LL E+C  F   +PC              T     + P+  S ++ C QH L+++GD+ RY+      N    A  YY     L PS G  YNQLA L +     L   ++Y ++      F  A  NL
Sbjct:   53 LGPAEVWTSRQALQDLYQKMLVTD-LEYALDKKVEQDLWNHAFKNQITTLQGQAKNRAN--PNRSEVQANLSLFLEAASGFYT----------QLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYIC-QHCLVHLGDIARYR------NQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNL 255
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Fly
Match: Smg5 (gene:FBgn0019890 transcript:FBtr0343669)

HSP 1 Score: 75.0998 bits (183), Expect = 3.088e-13
Identity = 61/233 (26.18%), Postives = 106/233 (45.49%), Query Frame = 1 
Query:  220 EIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFT-------------NFVPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRY----KHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRL 867
            EI+  R  L +   ++IF +    G+K  + +W+  +YE I   K+    +     +    +   +R +   I  Y++L  R++  ++L  +   DF+                   S     + S E  S+A   I  SLL +GDL+RY    + D K + S+    +YY +   LNP++G A NQL TL     + L++ Y YL + +C   F  ++ NL  LF ++ + L
Sbjct:   73 EIEEKRSLLVQLCKQIIFKDYQSVGKKVREVMWRRGYYEFIAFVKKNWHKQCQDAATAQENMQKLERFLCAGIFNYKRLSARMEEIYDLDLKYLIDFSIISDGYISDMIGDTMESSHSGTHAVDKSLEAVSFALDTIHSSLLSLGDLHRYFLDFRIDSKLSISKEQVAKYYLEAFKLNPAIGMAQNQLGTLHYGQNHDLDSTYHYLYSLVCIIPFELSENNLNKLFAKHTEYL 305
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Fly
Match: Smg5 (gene:FBgn0019890 transcript:FBtr0080562)

HSP 1 Score: 75.0998 bits (183), Expect = 3.088e-13
Identity = 61/233 (26.18%), Postives = 106/233 (45.49%), Query Frame = 1 
Query:  220 EIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFT-------------NFVPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRY----KHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRL 867
            EI+  R  L +   ++IF +    G+K  + +W+  +YE I   K+    +     +    +   +R +   I  Y++L  R++  ++L  +   DF+                   S     + S E  S+A   I  SLL +GDL+RY    + D K + S+    +YY +   LNP++G A NQL TL     + L++ Y YL + +C   F  ++ NL  LF ++ + L
Sbjct:   73 EIEEKRSLLVQLCKQIIFKDYQSVGKKVREVMWRRGYYEFIAFVKKNWHKQCQDAATAQENMQKLERFLCAGIFNYKRLSARMEEIYDLDLKYLIDFSIISDGYISDMIGDTMESSHSGTHAVDKSLEAVSFALDTIHSSLLSLGDLHRYFLDFRIDSKLSISKEQVAKYYLEAFKLNPAIGMAQNQLGTLHYGQNHDLDSTYHYLYSLVCIIPFELSENNLNKLFAKHTEYL 305
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Fly
Match: Smg5 (gene:FBgn0019890 transcript:FBtr0080561)

HSP 1 Score: 75.0998 bits (183), Expect = 3.088e-13
Identity = 61/233 (26.18%), Postives = 106/233 (45.49%), Query Frame = 1 
Query:  220 EIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFT-------------NFVPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRY----KHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRL 867
            EI+  R  L +   ++IF +    G+K  + +W+  +YE I   K+    +     +    +   +R +   I  Y++L  R++  ++L  +   DF+                   S     + S E  S+A   I  SLL +GDL+RY    + D K + S+    +YY +   LNP++G A NQL TL     + L++ Y YL + +C   F  ++ NL  LF ++ + L
Sbjct:   73 EIEEKRSLLVQLCKQIIFKDYQSVGKKVREVMWRRGYYEFIAFVKKNWHKQCQDAATAQENMQKLERFLCAGIFNYKRLSARMEEIYDLDLKYLIDFSIISDGYISDMIGDTMESSHSGTHAVDKSLEAVSFALDTIHSSLLSLGDLHRYFLDFRIDSKLSISKEQVAKYYLEAFKLNPAIGMAQNQLGTLHYGQNHDLDSTYHYLYSLVCIIPFELSENNLNKLFAKHTEYL 305
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Fly
Match: Smg6 (gene:FBgn0039260 transcript:FBtr0084796)

HSP 1 Score: 62.003 bits (149), Expect = 3.037e-9
Identity = 41/119 (34.45%), Postives = 62/119 (52.10%), Query Frame = 1 
Query:  592 QHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMK-KSGSNITKSGLKIMR 945
            Q  L+ +GDL RY+ +       + A RYY +   L P  G  +NQLA +    +   +A Y+Y+++ L SNS   AKE+LL LF+   ++      ++S M   +GS   KS  K MR
Sbjct:  285 QRLLICLGDLTRYRVNHVKATDYMEAARYYQRAQELVPGNGAPFNQLAVISIYHHKRFDAVYYYVRSLLTSNSIQSAKESLLDLFDEIRRKYEETEMKQSPMHYAAGSKNQKS--KQMR 401
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Zebrafish
Match: smg5 (SMG5 nonsense mediated mRNA decay factor [Source:ZFIN;Acc:ZDB-GENE-050913-144])

HSP 1 Score: 163.31 bits (412), Expect = 2.228e-40
Identity = 126/439 (28.70%), Postives = 215/439 (48.97%), Query Frame = 1 
Query:  130 KTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFV-PCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDM----NHYDIKQLAAMSDDVLNILSFVLGFDRKLVSNLSSTD----------NGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQ-VSLKFDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLLFHRNENKEE 1398
            K L R V ET+ KL  +   K++   +   E  +LR KL+E   +L+FL P+++G+KAE+ +W+ ++YE I++ K   K+    I+S+S +  A++ H++  +G++Q L+L +Q+ ++L  + C D+T+   P +  ++    + +   WA       L+Y+GDL RY++++    +  LA R+Y+Q LS+ P +G  +NQL TL    +Y + A Y+YL+       F  A  NL  LF++  K    V  +E  MKK         L   R +S  IK L V  SF YL  +    N     +L ++   VL   + VL F   L ++ S +             V P +L  K+V   L +                  V  LK+  S ++  + +F L L+ H+ N   +R++ EL     D+  L  R +N ++
Sbjct:   18 KRLYRAVVETVHKLDIIISGKSSYREVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQVIKTNKKH----IHSRSALECAYRTHLIAGVGFFQHLLLYIQSHYQLELQDCIDWTHVTDPLIGRKKPVSATSKEMEWAQMACHRCLVYLGDLARYQNELAGVEAEQLAERFYHQALSVAPHVGMPFNQLGTLAGSKFYNVEATYYYLRCIQSETPFDGAYGNLKRLFDKASKMYHQVKKQE--MKK---------LSPSRQRSKDIKRLLV--SFMYLQSLLQPKNSLMETELTSLCQSVLEDFNLVL-FYLPLPAHGSQSASEEEEEHDSVGSVLPDSLIFKMVVICLMV------------------VHSLKRGASKQYSASIAFTLALFSHLVNHVNIRLQAELEEGESDVPAL--RTDNTDD 418

HSP 2 Score: 63.5438 bits (153), Expect = 1.429e-9
Identity = 36/117 (30.77%), Postives = 59/117 (50.43%), Query Frame = 1 
Query: 2590 KEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIE 2940
            + ++MR+MA+LRL+ E+S    S      +     + S SP        YL+PD   +C  L  ++ L     +I +IP+ VI  LD +K  + GAR  I++ E E    N +I+ +
Sbjct:  904 RNRLMRDMAQLRLQLEVSQLEGS------LQQPKAQSSMSP--------YLVPDTAVLCQHLGLLRQLAASGCFIIIIPRTVIDGLDMLKKENSGARDGIRFLETEFRKGNRYIRCQ 1006
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Zebrafish
Match: smg6 (SMG6 nonsense mediated mRNA decay factor [Source:ZFIN;Acc:ZDB-GENE-081107-52])

HSP 1 Score: 78.9518 bits (193), Expect = 3.481e-14
Identity = 63/233 (27.04%), Postives = 113/233 (48.50%), Query Frame = 1 
Query:  232 LRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELK--NKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPCVSTEEEKEISPELKSWAFSCI--QHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGS 912
            LR +L     R+I L  +EF   Q  + T+WK++FY+ I  F++ LK  N       K++++   +    GA+ ++  L+ +LQ+ F+          +++ C++        P  K+  ++ I  Q  ++  GD+ RY+     + +   A  +Y +   + P  G  YNQLA L   +   L+A Y+Y+++   SN    AKE+L+ LFE   KR  + ++R       GS
Sbjct:  732 LRAELLTIYERVI-LTDIEFSDSQNVDQTLWKNVFYQVIERFRQLLKDQNSDTAPQIKTMLMTILEE---GAV-FFDSLLQKLQSVFQF------KLQDYMDCMAIRAR----PLRKTVKYALISAQRCMICQGDIARYREQASESANYGKARSWYLKAQQIAPKNGRPYNQLALLAVYTKRKLDAVYYYMRSLAASNPILTAKESLMSLFEE-AKRKADQVERRLKQDSDGS 948
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Zebrafish
Match: smg7 (SMG7 nonsense mediated mRNA decay factor [Source:ZFIN;Acc:ZDB-GENE-030131-9122])

HSP 1 Score: 60.8474 bits (146), Expect = 1.030e-8
Identity = 56/215 (26.05%), Postives = 97/215 (45.12%), Query Frame = 1 
Query:  208 LSTFEIQALRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFEL-LPEMCPDFTNFVPCVSTEEEKE---ISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENL 834
            L   E+   R  L++   +++  + LE+   +K E  +W   F   I   + + KN+ N   ++S +       +  A G+Y +L+  L T F + LP  C   ++ +  +S ++      + P+  S ++ C QH L+++GD+ RY+      N    A  YY     L PS G  YNQLA L +     L   ++Y ++      F  A  NL
Sbjct:   23 LGAAEVWTSRQALQDLYQKMLVTD-LEYALDKKVEQDLWNHAFKNQITTLQSQAKNRANP--NRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLP--CRVKSSQLGIISNKQSSTSAIVKPQPSSCSYIC-QHCLVHLGDIARYR------NQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNL 225
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Zebrafish
Match: smg7 (SMG7 nonsense mediated mRNA decay factor [Source:ZFIN;Acc:ZDB-GENE-030131-9122])

HSP 1 Score: 60.4622 bits (145), Expect = 1.154e-8
Identity = 56/215 (26.05%), Postives = 97/215 (45.12%), Query Frame = 1 
Query:  208 LSTFEIQALRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFEL-LPEMCPDFTNFVPCVSTEEEKE---ISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENL 834
            L   E+   R  L++   +++  + LE+   +K E  +W   F   I   + + KN+ N   ++S +       +  A G+Y +L+  L T F + LP  C   ++ +  +S ++      + P+  S ++ C QH L+++GD+ RY+      N    A  YY     L PS G  YNQLA L +     L   ++Y ++      F  A  NL
Sbjct:   23 LGAAEVWTSRQALQDLYQKMLVTD-LEYALDKKVEQDLWNHAFKNQITTLQSQAKNRANP--NRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLP--CRVKSSQLGIISNKQSSTSAIVKPQPSSCSYIC-QHCLVHLGDIARYR------NQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNL 225
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Xenopus
Match: smg5 (SMG5 nonsense mediated mRNA decay factor [Source:NCBI gene;Acc:780078])

HSP 1 Score: 72.4034 bits (176), Expect = 3.366e-12
Identity = 36/117 (30.77%), Postives = 61/117 (52.14%), Query Frame = 1 
Query: 2590 KEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIE 2940
            + ++MR+MA+LRL+ E+S                +   + P   +  + YL+PD + +C  L  I+ L    R+I +IP+ VI  LD +K  + GAR  I+Y E E +  N +I+ +
Sbjct:  572 RNRLMRDMAQLRLQLEVS--------------QLEGTLQQPKAQSAMSPYLVPDTQALCQHLGVIRQLATSGRFILIIPRTVIDGLDFLKKENAGARDGIRYLEAEFKKGNRYIRCQ 674
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Xenopus
Match: smg5 (SMG5 nonsense mediated mRNA decay factor [Source:NCBI gene;Acc:780078])

HSP 1 Score: 72.0182 bits (175), Expect = 3.867e-12
Identity = 36/115 (31.30%), Postives = 60/115 (52.17%), Query Frame = 1 
Query: 2590 KEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIK 2934
            + ++MR+MA+LRL+ E+S                +   + P   +  + YL+PD + +C  L  I+ L    R+I +IP+ VI  LD +K  + GAR  I+Y E E +  N +I+
Sbjct:  458 RNRLMRDMAQLRLQLEVS--------------QLEGTLQQPKAQSAMSPYLVPDTQALCQHLGVIRQLATSGRFILIIPRTVIDGLDFLKKENAGARDGIRYLEAEFKKGNRYIR 558
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Xenopus
Match: smg5 (SMG5 nonsense mediated mRNA decay factor [Source:NCBI gene;Acc:780078])

HSP 1 Score: 72.0182 bits (175), Expect = 4.140e-12
Identity = 36/117 (30.77%), Postives = 61/117 (52.14%), Query Frame = 1 
Query: 2590 KEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIE 2940
            + ++MR+MA+LRL+ E+S                +   + P   +  + YL+PD + +C  L  I+ L    R+I +IP+ VI  LD +K  + GAR  I+Y E E +  N +I+ +
Sbjct:  605 RNRLMRDMAQLRLQLEVS--------------QLEGTLQQPKAQSAMSPYLVPDTQALCQHLGVIRQLATSGRFILIIPRTVIDGLDFLKKENAGARDGIRYLEAEFKKGNRYIRCQ 707
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Xenopus
Match: smg5 (SMG5 nonsense mediated mRNA decay factor [Source:NCBI gene;Acc:780078])

HSP 1 Score: 72.0182 bits (175), Expect = 4.678e-12
Identity = 36/117 (30.77%), Postives = 61/117 (52.14%), Query Frame = 1 
Query: 2590 KEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIE 2940
            + ++MR+MA+LRL+ E+S                +   + P   +  + YL+PD + +C  L  I+ L    R+I +IP+ VI  LD +K  + GAR  I+Y E E +  N +I+ +
Sbjct:  577 RNRLMRDMAQLRLQLEVS--------------QLEGTLQQPKAQSAMSPYLVPDTQALCQHLGVIRQLATSGRFILIIPRTVIDGLDFLKKENAGARDGIRYLEAEFKKGNRYIRCQ 679
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Xenopus
Match: mgme1 (mitochondrial genome maintenance exonuclease 1 [Source:Xenbase;Acc:XB-GENE-1008562])

HSP 1 Score: 64.3142 bits (155), Expect = 1.395e-9
Identity = 62/249 (24.90%), Postives = 114/249 (45.78%), Query Frame = 1 
Query:  223 IQALRYKLKECLNRLIFLEPLEFGQK--AEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLG-AIGYYQKLILRLQTEFELLPEMCPDFTNFVPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFE---RNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVK 951
            + +LR +L +   R + L  +EF  +   +  +WK++FY+ I  F+  LK      +S S  I      VL     ++  L+ +LQ  ++   E   ++ + +   S    K +      +A    Q  ++  GD+ RY+     T++   A  +Y +   + P  G  YNQLA L   +   L+A Y+Y+++   SN    AKE+L+ LFE   R   +L     +E  +  +  + T+S + I  ++
Sbjct:  613 MASLRSELLQVYERCL-LSDIEFSDQHNVDQLLWKNVFYQVIEKFRHFLKE----TSSDSEEIRRKLLQVLDEGTAFFDSLLQKLQVTYQFKLE---EYMDGMAIRSKPLRKMVK-----FALISAQRCMICQGDIARYREQAADTSNYGKARSWYLKAQQIAPKNGRPYNQLALLAIYTRRKLDAVYYYMRSLAASNPILTAKESLMSLFEETKRKADQLEQKQQKERGVSHTSDDTTRSEIWIHPIR 848
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Mouse
Match: Smg5 (Smg-5 homolog, nonsense mediated mRNA decay factor (C. elegans) [Source:MGI Symbol;Acc:MGI:2447364])

HSP 1 Score: 166.777 bits (421), Expect = 1.826e-41
Identity = 148/541 (27.36%), Postives = 251/541 (46.40%), Query Frame = 1 
Query:  130 KTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFV-PCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDMNHYDIKQLAAMSDDVLNILSFVLGFDRKLVSNLSSTDNGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLK-FDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLLFHRNENKEEISVIKETCDEISSSKSTVAISPDHSVVKSRKKRVGLSKRLVRHRHDTDEASIEEDLSEDDQNLSENDNILWDDQISDSSDSNVGD--EG-GFGFSDVEDETDFESNEEIHSQDEDD 1737
            K L R V E + +L  +   K A + +   E  +LR KL+E   +L+FL P+++G+KAE+ +W+ ++YE I++ K   K+    I+S+S +  A++ H++  IG+YQ L+L +Q+ ++L  + C D+T+   P +  ++    S +   WA       L+Y+GDL RY++++   ++ +LA R+Y Q LS+ P +G  +NQL TL    YY + A Y YL+      SF  A  NL  L+++  K          + K     ++ S  +   +K  ++ ++   Y  + L   +     +L ++   VL   +  L F      NL  T+            +  LL    + + +M          V  LK+   K +  A +F L L+ H+ N   +R++ EL      +S  F  +   E  S  KE  ++         + P     +  +K   LS    R RH   +A  + DLSE  ++ S +D+     Q SD SDS      EG G  F   + ETD E N +    D +D
Sbjct:   21 KRLYRAVVEAVHRLDLILCNKAAYQEVFKPENVSLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKH----IHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLMGFKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQ------LKKSETRKLSPSKKRCKDIKRLLVNFM---YLQSLLQPKSSSVDSELTSLCQSVLEDFNLCL-FYLPSSPNLGLTNEDEEECESGYAFLPDLLIFQMAIICLM---------GVHSLKRAGSKHYSAAIAFTLALFSHLINHVNIRLQAELEEGENPVS-AFQSDGTDEPES--KEALEKEEPEPEPPTVVPQADEGRKSRKHSRLSCLRRRRRHHPPKAGDDSDLSEGFESDSSHDSA----QASDGSDSGSDKSLEGRGTAF---DAETDSEMNSQESRSDLED 528

HSP 2 Score: 70.4774 bits (171), Expect = 1.046e-11
Identity = 38/117 (32.48%), Postives = 60/117 (51.28%), Query Frame = 1 
Query: 2590 KEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIE 2940
            + ++MR+MA+LRL+ E+S    S               + P   +  + YLIPD + +C  L  I+ L    R+I +IP+ VI  LD +K    GAR  I+Y E E +  N +I+ +
Sbjct:  822 RNRLMRDMAQLRLQLEVSQLEGS--------------LQQPKAQSAMSPYLIPDTQALCYHLPLIRQLATSGRFIIIIPRTVIDGLDLLKKEQPGARDGIRYLEAEFKKGNRYIRCQ 924
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Mouse
Match: Smg5 (Smg-5 homolog, nonsense mediated mRNA decay factor (C. elegans) [Source:MGI Symbol;Acc:MGI:2447364])

HSP 1 Score: 129.028 bits (323), Expect = 1.237e-33
Identity = 63/183 (34.43%), Postives = 113/183 (61.75%), Query Frame = 1 
Query:  244 LKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFV-PCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLK 789
            L+E   +L+FL P+++G+KAE+ +W+ ++YE I++ K   K+    I+S+S +  A++ H++  IG+YQ L+L +Q+ ++L  + C D+T+   P +  ++    S +   WA       L+Y+GDL RY++++   ++ +LA R+Y Q LS+ P +G  +NQL TL    YY + A Y YL+
Sbjct:    2 LRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKH----IHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLMGFKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLR 180
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Mouse
Match: Smg6 (Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) [Source:MGI Symbol;Acc:MGI:2144117])

HSP 1 Score: 72.0182 bits (175), Expect = 9.095e-13
Identity = 67/250 (26.80%), Postives = 115/250 (46.00%), Query Frame = 1 
Query:  232 LRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLN 975
            LR +L +   R I L+ +EF   Q  +  +WK+ FY+ I  F++ LK+ PN  N + +     +    G+  ++  L+ +LQ  ++   E   D+ + +   S    K +      +A    Q S++  GD+ RY+     T +   A  +Y +   + P  G  YNQLA L   +   L+A Y+Y+++   SN    AKE+L+ LFE   ++       E M KK       S  K  + K S  +++ 
Sbjct:   76 LRTELLQLYERCILLD-IEFSDSQNVDQILWKNAFYQVIEKFRQLLKD-PNSENPEQIRNRLLELLDEGS-DFFDSLLQKLQVTYKFKLE---DYMDGLAIRSKPLRKTVK-----YALISAQRSMICQGDISRYREQANDTANYGKARSWYLKAQHIAPKNGRPYNQLALLAVYTRRKLDAVYYYMRSLAASNPILTAKESLMSLFEETKRKA------EQMEKKQHEEFDMSPDKWRKGKKSTFRHVG 308
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Mouse
Match: Smg6 (Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) [Source:MGI Symbol;Acc:MGI:2144117])

HSP 1 Score: 73.9442 bits (180), Expect = 1.213e-12
Identity = 80/300 (26.67%), Postives = 135/300 (45.00%), Query Frame = 1 
Query:  232 LRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKY-------LNVW----YSFAYLNDMNHYDIKQ---LAAMSDDVLN---ILSFV 1074
            LR +L +   R I L+ +EF   Q  +  +WK+ FY+ I  F++ LK+ PN  N + +     +    G+  ++  L+ +LQ  ++   E   D+ + +   S    K +      +A    Q S++  GD+ RY+     T +   A  +Y +   + P  G  YNQLA L   +   L+A Y+Y+++   SN    AKE+L+ LFE   ++       E M KK       S  K  + K S  ++       L +W    +S +     +  D +Q   L ++S   LN   ILSF+
Sbjct:  614 LRTELLQLYERCILLD-IEFSDSQNVDQILWKNAFYQVIEKFRQLLKD-PNSENPEQIRNRLLELLDEGS-DFFDSLLQKLQVTYKFKLE---DYMDGLAIRSKPLRKTVK-----YALISAQRSMICQGDISRYREQANDTANYGKARSWYLKAQHIAPKNGRPYNQLALLAVYTRRKLDAVYYYMRSLAASNPILTAKESLMSLFEETKRKA------EQMEKKQHEEFDMSPDKWRKGKKSTFRHVGDDTTRLEIWIHPSHSRSAQGTESGKDSEQENGLGSLSPSDLNKRFILSFL 896
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Mouse
Match: Smg7 (Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) [Source:MGI Symbol;Acc:MGI:2682334])

HSP 1 Score: 59.3066 bits (142), Expect = 3.078e-8
Identity = 56/223 (25.11%), Postives = 91/223 (40.81%), Query Frame = 1 
Query:  208 LSTFEIQALRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPC------------VSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENL 834
            L   E+   R  L++   +++  + LE+   +K E  +W   F   I   + + KN+ N   ++S +       +  A G+Y           +LL E+C  F   +PC              T     + P+  S ++ C QH L+++GD+ RY+      N    A  YY     L PS G  YNQLA L +     L   ++Y ++      F  A  NL
Sbjct:   24 LGPAEVWTSRQALQDLYQKMLVTD-LEYALDKKVEQDLWNHAFKNQITTLQGQAKNRAN--PNRSEVQANLSLFLEAASGFYT----------QLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIVKPQSSSCSYIC-QHCLVHLGDIARYR------NQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNL 226
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. UniProt/SwissProt
Match: sp|Q6ZPY2|SMG5_MOUSE (Protein SMG5 OS=Mus musculus OX=10090 GN=Smg5 PE=1 SV=2)

HSP 1 Score: 166.777 bits (421), Expect = 1.278e-40
Identity = 148/541 (27.36%), Postives = 251/541 (46.40%), Query Frame = 1 
Query:  130 KTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFV-PCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDMNHYDIKQLAAMSDDVLNILSFVLGFDRKLVSNLSSTDNGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLK-FDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLLFHRNENKEEISVIKETCDEISSSKSTVAISPDHSVVKSRKKRVGLSKRLVRHRHDTDEASIEEDLSEDDQNLSENDNILWDDQISDSSDSNVGD--EG-GFGFSDVEDETDFESNEEIHSQDEDD 1737
            K L R V E + +L  +   K A + +   E  +LR KL+E   +L+FL P+++G+KAE+ +W+ ++YE I++ K   K+    I+S+S +  A++ H++  IG+YQ L+L +Q+ ++L  + C D+T+   P +  ++    S +   WA       L+Y+GDL RY++++   ++ +LA R+Y Q LS+ P +G  +NQL TL    YY + A Y YL+      SF  A  NL  L+++  K          + K     ++ S  +   +K  ++ ++   Y  + L   +     +L ++   VL   +  L F      NL  T+            +  LL    + + +M          V  LK+   K +  A +F L L+ H+ N   +R++ EL      +S  F  +   E  S  KE  ++         + P     +  +K   LS    R RH   +A  + DLSE  ++ S +D+     Q SD SDS      EG G  F   + ETD E N +    D +D
Sbjct:   21 KRLYRAVVEAVHRLDLILCNKAAYQEVFKPENVSLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKH----IHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLMGFKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQ------LKKSETRKLSPSKKRCKDIKRLLVNFM---YLQSLLQPKSSSVDSELTSLCQSVLEDFNLCL-FYLPSSPNLGLTNEDEEECESGYAFLPDLLIFQMAIICLM---------GVHSLKRAGSKHYSAAIAFTLALFSHLINHVNIRLQAELEEGENPVS-AFQSDGTDEPES--KEALEKEEPEPEPPTVVPQADEGRKSRKHSRLSCLRRRRRHHPPKAGDDSDLSEGFESDSSHDSA----QASDGSDSGSDKSLEGRGTAF---DAETDSEMNSQESRSDLED 528

HSP 2 Score: 70.4774 bits (171), Expect = 7.314e-11
Identity = 38/117 (32.48%), Postives = 60/117 (51.28%), Query Frame = 1 
Query: 2590 KEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIE 2940
            + ++MR+MA+LRL+ E+S    S               + P   +  + YLIPD + +C  L  I+ L    R+I +IP+ VI  LD +K    GAR  I+Y E E +  N +I+ +
Sbjct:  822 RNRLMRDMAQLRLQLEVSQLEGS--------------LQQPKAQSAMSPYLIPDTQALCYHLPLIRQLATSGRFIIIIPRTVIDGLDLLKKEQPGARDGIRYLEAEFKKGNRYIRCQ 924
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. UniProt/SwissProt
Match: sp|Q9UPR3|SMG5_HUMAN (Protein SMG5 OS=Homo sapiens OX=9606 GN=SMG5 PE=1 SV=3)

HSP 1 Score: 152.91 bits (385), Expect = 3.223e-36
Identity = 143/534 (26.78%), Postives = 244/534 (45.69%), Query Frame = 1 
Query:  130 KTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFV-PCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDMNHYDIKQLAAMSDDVL---NILSFVLGFDRKLVSNLSSTDNGVRPST---LNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLKFDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLLFHRNENKEEISVIKETCDEISSSKSTVAISPDHSVVKSRKKRVGLSKRLVRHRHDTDEASIEEDLSEDDQNLSENDNILWDDQISDSSDSNVGDEGGFGFSDVEDETDFESNE 1710
            K L R V E + +L  +   K A + +   E  +LR KL+E   +L+FL P+++G+KAE+ +W+ ++YE I++ K   K+    I+S+S +  A++ H++  IG+YQ L+L +Q+ ++L  + C D+T+   P +  ++    S +   WA       L+Y+GDL RY++++   ++ +LA R+Y Q LS+ P +G  +NQL TL    YY + A Y YL+      SF  A  NL  L+++  K           +KK  +     G K  R K      +N  Y  + L   +     +L ++   VL   N+  F L     L  +L+S D     S    L   ++ Q++ I   C++ +              +  S ++  A +F L L+ H+ N   +R++ EL      +   F  +   E  S      +E    +            KSRK       R  RH     + S   DLSE  ++ S +D+    +     SD ++  EGG    D E +++  S E
Sbjct:   21 KRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKH----IHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQ-------LKKCETRKLSPGKK--RCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNL--SLASEDEEEYESGYAFLPDLLIFQMVIICLMCVHSL-------------ERAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVP-AFQSDGTDEPESKEPVEKEEEPDPEPPPVTPQVGEGRKSRKFSRLSCLRRRRHPPKVGDDS---DLSEGFESDSSHDSARASEGSDSGSDKSL--EGGGTAFDAETDSEMNSQE 520

HSP 2 Score: 71.2478 bits (173), Expect = 4.988e-11
Identity = 37/117 (31.62%), Postives = 61/117 (52.14%), Query Frame = 1 
Query: 2590 KEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIE 2940
            + ++MR+MA+LRL+ E+S    S               + P   +  + YL+PD + +C+ L  I+ L    R+I +IP+ VI  LD +K    GAR  I+Y E E +  N +I+ +
Sbjct:  821 RNRLMRDMAQLRLQLEVSQLEGS--------------LQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQ 923
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. UniProt/SwissProt
Match: sp|P61406|EST1A_MOUSE (Telomerase-binding protein EST1A OS=Mus musculus OX=10090 GN=Smg6 PE=1 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 8.484e-12
Identity = 80/300 (26.67%), Postives = 135/300 (45.00%), Query Frame = 1 
Query:  232 LRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKY-------LNVW----YSFAYLNDMNHYDIKQ---LAAMSDDVLN---ILSFV 1074
            LR +L +   R I L+ +EF   Q  +  +WK+ FY+ I  F++ LK+ PN  N + +     +    G+  ++  L+ +LQ  ++   E   D+ + +   S    K +      +A    Q S++  GD+ RY+     T +   A  +Y +   + P  G  YNQLA L   +   L+A Y+Y+++   SN    AKE+L+ LFE   ++       E M KK       S  K  + K S  ++       L +W    +S +     +  D +Q   L ++S   LN   ILSF+
Sbjct:  614 LRTELLQLYERCILLD-IEFSDSQNVDQILWKNAFYQVIEKFRQLLKD-PNSENPEQIRNRLLELLDEGS-DFFDSLLQKLQVTYKFKLE---DYMDGLAIRSKPLRKTVK-----YALISAQRSMICQGDISRYREQANDTANYGKARSWYLKAQHIAPKNGRPYNQLALLAVYTRRKLDAVYYYMRSLAASNPILTAKESLMSLFEETKRKA------EQMEKKQHEEFDMSPDKWRKGKKSTFRHVGDDTTRLEIWIHPSHSRSAQGTESGKDSEQENGLGSLSPSDLNKRFILSFL 896
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. UniProt/SwissProt
Match: sp|Q5RAK6|EST1A_PONAB (Telomerase-binding protein EST1A OS=Pongo abelii OX=9601 GN=SMG6 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 7.871e-11
Identity = 74/298 (24.83%), Postives = 131/298 (43.96%), Query Frame = 1 
Query:   97 IRIRQNASVKEKTLI---RVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLN 975
            +R+  N  ++   L+   R+  E L+K+ QL       E+L  +E             R I L+ +EF   Q  +  +WK+ FY+ I  F R+L   PN+ N + +     +    G+  ++  L+ +LQ  ++   E   D+ + +   S    K +      +A    Q  ++  GD+ RY+   + T +   A  +Y +   + P  G  YNQLA L   +   L+A Y+Y+++   SN    AKE+L+ LFE   ++       E M KK       S  +  + K S  +++ 
Sbjct:  585 LRVADNQELQLSNLLSRDRISPEGLEKMAQLRA-----ELLQLYE-------------RCILLD-IEFSDNQNVDQILWKNAFYQVIEKF-RQLVKDPNVENPEQIRNRLLELLDEGS-DFFDSLLQKLQVTYKFKLE---DYIDGLAIRSKPLRKTVK-----YALISAQRCMICQGDIARYREQARDTANYGKARSWYLKAQHIAPKNGRPYNQLALLAVYTRRKLDAVYYYMRSLAASNPILTAKESLMSLFEETKRKA------EQMEKKQHEEFELSPDQWRKGKKSTFRHVG 847
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. UniProt/SwissProt
Match: sp|Q86US8|EST1A_HUMAN (Telomerase-binding protein EST1A OS=Homo sapiens OX=9606 GN=SMG6 PE=1 SV=2)

HSP 1 Score: 69.3218 bits (168), Expect = 1.948e-10
Identity = 74/298 (24.83%), Postives = 130/298 (43.62%), Query Frame = 1 
Query:   97 IRIRQNASVKEKTLI---RVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLN 975
            +R+  N  ++   L+   R+  E L+K+ QL       E+L  +E             R I L+ +EF   Q  +  +WK+ FY+ I  F R+L   PN+ N + +     +    G+  ++  L+ +LQ  ++   E   D+ + +   S    K +      +A    Q  ++  GD+ RY+     T +   A  +Y +   + P  G  YNQLA L   +   L+A Y+Y+++   SN    AKE+L+ LFE   ++       E M KK       S  +  + K S  +++ 
Sbjct:  585 LRVADNQELQLSNLLSRDRISPEGLEKMAQLRA-----ELLQLYE-------------RCILLD-IEFSDNQNVDQILWKNAFYQVIEKF-RQLVKDPNVENPEQIRNRLLELLDEGS-DFFDSLLQKLQVTYKFKLE---DYMDGLAIRSKPLRKTVK-----YALISAQRCMICQGDIARYREQASDTANYGKARSWYLKAQHIAPKNGRPYNQLALLAVYTRRKLDAVYYYMRSLAASNPILTAKESLMSLFEETKRKA------EQMEKKQHEEFDLSPDQWRKGKKSTFRHVG 847
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. TrEMBL
Match: A0A151NY35 (Protein SMG5 OS=Alligator mississippiensis OX=8496 GN=SMG5 PE=4 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 3.484e-50
Identity = 244/1004 (24.30%), Postives = 421/1004 (41.93%), Query Frame = 1 
Query:  121 VKEKTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFV-PCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDMNHYDIKQLAAMSDDVLNILSFVLGFDRKLVSNLSSTDNGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLK-FDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLL----FHRNENKEEISVIKETCDEISSSKSTVAISPDHSVVKSRKKRVGLSKRLVRHRHDTDEASIEEDLSEDDQNLSENDNILWDDQISDSSDSNVGDEGGFGFSDVEDET--DFESNEEIHSQD-----EDDQIIRAIVGHN--TIFGRNQSLNGKDLKRSRD------------KESANNSLVNKVIFSETLIHES-------SQSIKPPKRRSATSQGVFNQFNNVKPSAEALVTCIFNVDLLQSTSVVISAFNHFINDKLESFCRVWQYASYLWKHFAVMLNFCENILDDLEQNDLTGIQ-CNH------ATEKIRLLPPCYLPEDAEL-NLLSVEHADKSIVLCEN-----SLHQVVTRALRIIQCAHSIASISQGPIALQKINSSNLWLFSYSHNSPRTNLVFKSWTQIFSNSDSHLQGNPNSVAITTMSSSINAPSPAVVSAVDKEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIESTAT-------LNNQDL 2970
             +++ L   V E + +L  +   K A + +   E  +LR KL+E   +L+FL P+E+G+KAE+ +W+ ++YE I++ K   K+    I+S+S +  A++ H++  IG+YQ L+L +Q+ ++L  + C D+T+   P +  ++    S +   WA       L+Y+GDL RY++++   ++ +LA R+Y Q LS+ P +G  +NQL TL    YY + A Y YL+      SF  A  NL  L+++  K    +   ES        ++ S  +   +K  ++ ++   Y  + L   +     +L ++   VL   +  L F      NLSS              +  LL      + +M          V  LK+   K +  A +F L L+ H+ N   +R++ EL      +           E +E ++ I++  +   ++    A    +   K +K       R  RH    DE+ + E    D  +              DS+  + G E G   SD E E   D E++ +++SQ+     ED +     VG N  +I    Q+++ +  +  R             K ++  + + + + S+  I+          Q        S TS    N+    + S E  +  + N  LL +  V +       N  L   C   Q +  LW   +V+LN    +L    Q    G+  CN        +E   L     LPED  L NL  +++A K     ++      + + V R   I    H I  + QG I LQ   +S L +F     S + NL+ ++  Q       H+                            + ++MR+MA+LRL+ E+S    S               + P   +  + YL+PD + +C  L  ++ L    R+I +IP+ VI  LD +K  + GAR +I+Y E E +  N +I+ +  A        L  QDL
Sbjct:    6 TEKEALALAVVEAVHRLDLILGNKAAYQEVFKPENISLRNKLRELCVKLMFLHPVEYGRKAEELLWRKVYYEVIQLIKTNKKH----IHSRSTLECAYRTHLIAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASEKEMEWAQMACHRCLVYLGDLARYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEATYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCES------RKLSPSKKRCKDIKRLLVSFM---YLQSLLQPKSSSVDSELTSLCQSVLEDFNLCL-FYLPSPPNLSSASEDEEEYESGYSFLPDLLIFRMVIVCLM---------SVHSLKRAGSKQYSAAIAFTLALFSHLINHVNIRLQAELEEGENPVPAFQSDGTDDQEPREPVNSIEKEAEADLANHQPSAEQQKNDGKKGKKYSRLSCLRRRRHPQKVDESDLSEGFDSDSSH--------------DSAKGSDGSESGSEKSDEEAEAAFDVETDSDMNSQESRSDLEDMEDEPGSVGTNEASIASNLQAMSTQLFQTKRCFRLAPTFSNVLLKPTSGTTALKEGVGSKPCINGDVNKPDLVEQDDMSDSEESVTS----NKSCRNEKSLEEKLEILTNEGLLPTVKVFLDWLR--TNTDLIIMCA--QSSQSLWNRLSVLLN----LLPSAAQLQEPGLALCNEVKDLLSGSELPDLSTHLLLPEDVALRNLPPLKNAHKRFNFEQDRPILSPVEESVIRICCIRSFGHFITRL-QGSI-LQ--FNSELGIFISIAQSEQDNLLHQAEAQF------HM----------------------AAEEARRNRLMRDMAQLRLQLEVSQLEGS--------------LQQPKAQSAMSPYLVPDTQALCQHLGIVRQLASSGRFILIIPRTVIDGLDFLKKENAGARDSIRYLEAEFKKGNRYIRCQKDAGKSFERHKLKRQDL 914
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. TrEMBL
Match: H0YZZ8 (PINc domain-containing protein OS=Taeniopygia guttata OX=59729 PE=4 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 4.286e-49
Identity = 246/1001 (24.58%), Postives = 416/1001 (41.56%), Query Frame = 1 
Query:  130 KTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFV-PCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVK---RLRNVLDRE-SMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDMNHYDIKQLAAMSDDVLNILSFVLGFDRKLVSNLSSTDNGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLK-FDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLLFHRNENKEEISVIKETCDEISSSKSTVAISPDHSVVKSRKKRVGLSKRLVRHRHDTDEASIEEDLSEDDQNLSENDNILWDDQISDSSDSNVGDEGGFGFSDVEDETDFESNEEIHS----QDEDDQIIRAIVGHNTIFGRNQSLNGKDLKRSRDKESANN--SLVNKVI-----------FSETLIHESSQSIKPPKRRSAT-SQGVFN---------QFNNVKPSAEALV---TCIFNVDLLQSTSVV--------ISAFNHFINDKLESFCRVWQYASYLWKHFAVMLN--------------FCENILDDLEQNDLTGIQCNHATEKIRLLPPCYLPEDAEL-NLLSVEHADKSIVLCEN-----SLHQVVTRALRIIQCAHSIASISQGPIALQKINSSNLWLFSYSHNSPRTNLVFKSWTQIFSNSDSHLQGNPNSVAITTMSSSINAPSPAVVSAVDKEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIE 2940
            K L R V E + +L  +   K A + +   E  +LR KL+E   +L+FL P+++G+KAE+ +W+ ++YE I++ K     K   I+S+S +  A++ H++  IG+YQ L+L +Q+ ++L  + C D+T+   P +  ++    S +   WA       L+Y+GDL RY++++   ++ +LA R+Y Q LS+ P +G  +NQL TL    YY + A Y YL+      SF  A  NL  L+++  K   +L+    R+ S  KK G +I +  +  M ++S              L   +     +L ++   VL   +  L F      NLSST             +  LL      + +M          V  LK+   K +  A +F L L+ H+ N   +R++ EL      +   F  +   E+     +  +   ++         +   KS+K       R  RH    DE+    DLSE   + S   +I   D     S S   DE      DVE ++D  S E        +DE  +   +            S+NG     + D   A+N  ++  ++            FS  L+  SS    PP  +    S+   N         + ++V  S E++    +C     L +   +V        +  F  ++    +      Q +  LW   +V+LN               C+ + D L   +L  ++ N       LL    LPED  L NL  +++A K     ++     ++ + V R   I    H I  + QG I LQ   +S L +F     S + NL+ ++  Q       H+                            + ++MR+MA+LRL+ E+S    S               + P   +  + YL+PD + +C  L  +K L    R+I +IP+ VI  LD +K  + GAR +I+Y E E +  N +I+ +
Sbjct:   22 KRLYRAVVEAVHRLDLILGNKAAYQDVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKT--NKKAAHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASEKEMEWAQMACHRCLVYLGDLARYQNELAGVDTELLAERFYYQALSVAPQVGMPFNQLGTLAGSKYYNVEATYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPSKKRGKDIKRLLVSFMYLQS-------------LLQPKSSSLDSELTSLCQSVLEDFNLCL-FYLPSPPNLSSTSEDEEEYESGYSFLPDLLIFRMVIICLM---------SVHSLKRAGSKQYSAAIAFTLALFSHLINHVNIRLQAELEEGENPVP-AFQSDGTDEQEPREAKEAEAELANHQPSEEQRKNDCKKSKKYSRLSCLRRRRHPQKVDES----DLSEGFDSDSSQSSIKGSD--GSESGSEKSDEEAEAAFDVETDSDMNSQESRSDLEDMEDEPGEEGSSQGKSGPKEALKNSVNGPASLSTNDASIASNLQAMSTQLFQTKRCFRLAPTFSNILLKPSSD---PPALKGGIESKPCVNGDLEKPSLAEQDDVSDSEESISSNKSCRNERSLQEKLEIVTNEGLLLTVKVFLDWLRTNTDLIIMCAQSSQSLWNRLSVLLNLLPSAADLQESGLALCDEVKDILNDAELPDLKAN-------LL----LPEDVALRNLPPLKNAHKRFNFEQDRPIFSAVEESVVRICCIRSFGHFITHL-QGSI-LQ--FNSELGIFISIAQSEQDNLLHQAQAQF------HM----------------------AAEEARRNRLMRDMAQLRLQLEVSQLEGS--------------LQQPKAQSAMSPYLVPDTQALCQHLALVKQLATSGRFIIIIPRTVIDGLDFLKKENAGARDSIRYLEAEFKKGNRYIRCQ 930
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. TrEMBL
Match: A0A3P9CGU1 (SMG5 nonsense mediated mRNA decay factor OS=Maylandia zebra OX=106582 PE=4 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 4.306e-49
Identity = 244/1003 (24.33%), Postives = 410/1003 (40.88%), Query Frame = 1 
Query:  130 KTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFV-PCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDM----NHYDIKQLAAMSDDVL---NILSFVL-----GFDRKLVSNL-------SSTDNGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLK-FDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLLFHRNENKEEISV---IKETCDEISSSKSTVAISPDHSVVKSRKKRVGLSKRLVRHRHDTDEASIEEDLSED----------DQNLSENDNILWDDQISDSSDSNVGDEGGFGFSDVEDETDFESNEEIHSQDEDDQIIRAIVGHNTIFGRNQSLNGKDLKRSRDKESANNSLVNKVI-----------FSETLI--HESSQSIKPPKRRSATSQGVFNQFNNVKPSAEALVTCIFNVDLLQSTSVVISAFNHFINDKLESFCRVWQYASYLWKHFAVMLNFCENILDDLEQNDL----TGIQCNHATEKIRLLPPCYLPEDAELNLLSVEHADKSIVLCENSLHQVVTRALRIIQCAHSIASISQGPIALQKINSSNLWLFSYSHNSPRTNLVFKSWTQIFSNSDSHLQGNPNSVAITTMSSSINAPSPAVVSAVDKEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIE--STATLNNQDL-AC 2976
            K L R V ET+ KL  +   K++   +   E  +LR KL+E   +L+FL P+++G+KAE+ +W+ ++YE I++ K   K+    I+S+S +  A++ H++  +G+YQ L+L +Q+ ++L  + C D+T+   P +  ++    +P+   WA       L+Y+GDL RY++++    +  LA R+Y+Q LS+ P +G  +NQL TL    +Y + A Y+YL+       F  A  NL  LF++  K    V  +E  MKK         L   R +S  IK L V  SF YL  +    N     +L ++   VL   N++ F L     G      S         S +   V P  L  K+V   L +                  V  LK+   K +  + +F L L+ H+ N   +R++ EL  +   +  L      + EIS+    K   ++    K    +S       +RK   G  +  +    + + +S++++   D               E     +D  +   SD N   E      D+ED  + E +   H  DED+   +   G         + NG  +       S   ++ +++            FS  L+  H SS    P    +  S    +Q +     +E  +     +   Q    V+  F  ++    +      Q +  LW   +V+LN   +    LE  +L       +  +  E+  L+    LPED  L  L                  V  R L   +   S++ + +  + +  I S   +L S   N     L F     IF++     Q N        +     A          + ++MR+MA+LRL+ E+S    S      +     + S SP        YL+PD   +C  L+ I+ L     +I +IP+ VI  LDR+K  + GAR  I++ E E    N   ++E      L  QD+ AC
Sbjct:   21 KRLYRAVVETVHKLDVIIGSKSSYREVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQVIKTNKKH----IHSRSALECAYRTHLIAGVGFYQHLLLYIQSHYQLELQDCIDWTHVTDPLIGRKKPVSATPKEMEWAQMACHRCLVYLGDLARYQNELAGVEAEQLAERFYHQALSVMPHVGMPFNQLGTLAGSKFYNVEATYYYLRCIQSDAPFEGAYGNLKRLFDKAAKMYHQVKKQE--MKK---------LSPSRQRSKDIKRLLV--SFMYLQSLLQPKNSLMETELTSLCQSVLEDFNLVMFYLPPPTHGGAHHTPSEEEEEQQQHSDSSYPVLPDNLIFKMVVTCLMV------------------VHSLKRGGSKQYSASIAFTLALFSHLVNHVNIRLQAELEEAESQVPPLQTDAAGEREISIEEQRKMNEEKQRQKKKYSRLSRLRRRRCARKDGHGEDESDLSEGFEINSSSVKKEPKRDALGEEGSWGSGSEEEEEGGTAFD--VETDSDMN-SQESRSDLEDIEDTENSEGHAREHQDDEDEDQPKEDAGERDTTDTPPTTNGPLMSSDSSISSNLQAMSSQLFQAKRCFRLAPTFSNMLLRPHASSSPSIPTPTDNLDSDSEGSQHSTESVHSEKTLLEKLEIMTNQGLIQVVKVFLDWLRTNTDIILMCAQSSQSLWNRLSVLLNLLPDGSKLLEAGELLLNAEVTELLNECERPDLVQSLLLPEDLALRHLPA--------------LGVAHRRLDFSRRNPSLSPLQECVVRVCCIRSFGHFLTSLQGNV----LHFNPEAGIFTSVSQSKQDN--------LVQQAKAQFRMAEEEARRNRLMRDMAQLRLQLEVSQLEGS------LQQPKAQSSMSP--------YLVPDTAALCQHLNLIRQLAGSGCFIIIIPRTVIDGLDRLKKENAGARDGIRFLESEFRKGNRRSRVEVLKRDKLKRQDIEAC 945
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. TrEMBL
Match: A0A1D5PSK6 (PINc domain-containing protein OS=Gallus gallus OX=9031 GN=SMG5 PE=4 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 1.088e-47
Identity = 237/1013 (23.40%), Postives = 415/1013 (40.97%), Query Frame = 1 
Query:  130 KTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFV-PCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDMNHYDIKQLAAMSDDVLNILSFVLGFDRKLVSNLSSTDNGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLK-FDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLL----FHRNENKEEISVIKETCDEISSSKSTVAISPDHSVVKSRKKRVGLSKRLVRHRHDTDEASIEEDLSEDDQNLSENDNILWDDQISDSSDSNVGDEGGFG---------------FSDVEDETDFESNEEIHSQDEDDQIIRAIVGHNTIFGRNQS----------LNGKDLKRSRDKESANN--SLVNKVI-----------FSETLIHESSQSIKPPKRRSATSQGVFN---------QFNNVKPSAEALVT---CIFNVDLLQSTSVV--------ISAFNHFINDKLESFCRVWQYASYLWKHFAVMLNFCENILDDLEQNDLTGIQCNHATEKI------RLLPPCYLPEDAEL-NLLSVEHADKSIVLCEN-----SLHQVVTRALRIIQCAHSIASISQGPIALQKINSSNLWLFSYSHNSPRTNLVFKSWTQIFSNSDSHLQGNPNSVAITTMSSSINAPSPAVVSAVDKEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIE 2940
            K L R V E + +L  +   K A + +   E  +LR KL+E   +L+FL P+++G+KAE+ +W+ ++YE I++ K   K+    I+S+S +  A++ H++  IG+YQ L+L +Q+ ++L  + C D+T+   P +  ++    S +   WA       L+Y+GDL RY++++   ++ +LA R+Y Q LS+ P +G  +NQL TL    YY + A Y YL+      SF  A  NL  L+++  K  + +   ES        ++ S  +   +K  ++ ++   Y  + L   +     +L ++   VL   +  L F      NLSS              +  LL      +  M          V  L++   K +  A +F L L+ H+ N   +R++ EL      +           E +E +S +++  +    +         H   KS+K       R  RH    DE+ + E    D    S   +   D   S S  S+   E  F                  D+EDE   E  E   S    + +   + G  +   +N S          +NG     + D   A+N  ++  ++            FS  L+  S +  +P  +    S+   N         + +NV  S E++++   C     L +   +V        +  F  ++    +      Q +  LW   +V+LN   +  D L+ + L    C    E +       L     LPED  L NL  + +A + +   ++     +L + V R   I    H +  + QG I LQ   +  L +F     S R ++V ++  Q       H+                            + ++MR+MA+LRL+ E+S    S               + P   +  + YL+ D + +C  L  +K L    R+I +IP+AVI  LD +K  + GAR +I+Y E E +  N +I+ +
Sbjct:   22 KRLYRAVVEAVHRLDLILGNKAAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKH----IHSRSALECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLMGCKKPVSASEKEMEWAQMACHRCLVYLGDLARYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEATYCYLRCIQSEVSFEGASGNLKRLYDKAAKMYQQLKKCES------RKLSPSKKRCKDIKRLLVSFM---YLQSLLQPKSSSLDSELTSLCQSVLEDFNLCL-FYLPSPPNLSSVSEDEEEYESGYSFLPDLLIFRMVIICFM---------SVHSLRRAGSKQYSAAIAFTLALFSHLINHVNIRLQAELEEGENPVPAFRSDGTDEPEPREPLSSMEKEGEAELPNHHPREEERKHDCKKSKKYSRLSCLRRRRHPQKADESDLSEGFDSDSSQGSTKGS---DGSESGSEKSDEEAEAAFDVETDSDMNSQESRSDLEDMEDEPGKEGGERGRS-GAGEALKNGVDGGGSGECKNMSSSKTESADPAVNGPTSLSASDASIASNLQAMSTQLFQTKRCFRLAPTFSNILLKPSGE--QPASKGGLESKPCINGDVEKPGLAEQDNVSDSEESVLSTKSCRNERSLQEKLEIVSNEGLLLTVKVFLDWLRTNTDFIIMCAQSSQSLWNRLSVLLNLLPSAAD-LQDSGLE--LCKEVREVLSGAELPELRANLLLPEDVALRNLPPLRNAHRRLNFEQDRPIFSALEESVVRICCIRSFGHFVTRL-QGSI-LQ--FNPELGIFISIAQSERDSVVQQAQAQF------HM----------------------AAEEARRNRLMRDMAQLRLQLEVSQLEGS--------------LQQPKAQSAMSPYLVLDAQALCQHLAVVKQLAGSGRFIIIIPRAVIDSLDFLKKENAGARDSIRYLEAEFKKGNRYIRCQ 956
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. TrEMBL
Match: A0A1D5PME4 (PINc domain-containing protein OS=Gallus gallus OX=9031 GN=SMG5 PE=4 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 4.231e-47
Identity = 235/1010 (23.27%), Postives = 414/1010 (40.99%), Query Frame = 1 
Query:  139 IRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFV-PCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDMNHYDIKQLAAMSDDVLNILSFVLGFDRKLVSNLSSTDNGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLK-FDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLLFHRN----ENKEEISVIKETCDEISSSKSTVAISPDHSVVKSRKKRVGLSKRLVRHRHDTDEASIEEDLSEDDQNLSENDNILWDDQISDSSDSNVGDEGGFG---------------FSDVEDETDFESNEEIHSQDEDDQIIRAIVGHNTIFGRNQS----------LNGKDLKRSRDKESANN--SLVNKVI-----------FSETLIHESSQSIKPPKRRSATSQGVFN---------QFNNVKPSAEALVT---CIFNVDLLQSTSVV--------ISAFNHFINDKLESFCRVWQYASYLWKHFAVMLNFCENILDDLEQNDLTGIQCNHATEKI------RLLPPCYLPEDAEL-NLLSVEHADKSIVLCEN-----SLHQVVTRALRIIQCAHSIASISQGPIALQKINSSNLWLFSYSHNSPRTNLVFKSWTQIFSNSDSHLQGNPNSVAITTMSSSINAPSPAVVSAVDKEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIE 2940
            +R V E + +L  +   K A + +   E  +LR KL+E   +L+FL P+++G+KAE+ +W+ ++YE I++ K   K+    I+S+S +  A++ H++  IG+YQ L+L +Q+ ++L  + C D+T+   P +  ++    S +   WA       L+Y+GDL RY++++   ++ +LA R+Y Q LS+ P +G  +NQL TL    YY + A Y YL+      SF  A  NL  L+++  K  + +   ES        ++ S  +   +K  ++ ++   Y  + L   +     +L ++   VL   +  L F      NLSS              +  LL      +  M          V  L++   K +  A +F L L+ H+ N   +R++ EL      +           E +E +S +++  +    +         H   KS+K       R  RH    DE+ + E    D    S   +   D   S S  S+   E  F                  D+EDE   E  E   S    + +   + G  +   +N S          +NG     + D   A+N  ++  ++            FS  L+  S +  +P  +    S+   N         + +NV  S E++++   C     L +   +V        +  F  ++    +      Q +  LW   +V+LN   +  D L+ + L    C    E +       L     LPED  L NL  + +A + +   ++     +L + V R   I    H +  + QG I LQ   +  L +F     S R ++V ++  Q       H+                            + ++MR+MA+LRL+ E+S    S               + P   +  + YL+ D + +C  L  +K L    R+I +IP+AVI  LD +K  + GAR +I+Y E E +  N +I+ +
Sbjct:   16 MRAVVEAVHRLDLILGNKAAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKH----IHSRSALECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLMGCKKPVSASEKEMEWAQMACHRCLVYLGDLARYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEATYCYLRCIQSEVSFEGASGNLKRLYDKAAKMYQQLKKCES------RKLSPSKKRCKDIKRLLVSFM---YLQSLLQPKSSSLDSELTSLCQSVLEDFNLCL-FYLPSPPNLSSVSEDEEEYESGYSFLPDLLIFRMVIICFM---------SVHSLRRAGSKQYSAAIAFTLALFSHLINHVNIRLQAELEEGENPVPAFRSDGTDEPEPREPLSSMEKEGEAELPNHHPREEERKHDCKKSKKYSRLSCLRRRRHPQKADESDLSEGFDSDSSQGSTKGS---DGSESGSEKSDEEAEAAFDVETDSDMNSQESRSDLEDMEDEPGKEGGERGRS-GAGEALKNGVDGGGSGECKNMSSSKTESADPAVNGPTSLSASDASIASNLQAMSTQLFQTKRCFRLAPTFSNILLKPSGE--QPASKGGLESKPCINGDVEKPGLAEQDNVSDSEESVLSTKSCRNERSLQEKLEIVSNEGLLLTVKVFLDWLRTNTDFIIMCAQSSQSLWNRLSVLLNLLPSAAD-LQDSGLE--LCKEVREVLSGAELPELRANLLLPEDVALRNLPPLRNAHRRLNFEQDRPIFSALEESVVRICCIRSFGHFVTRL-QGSI-LQ--FNPELGIFISIAQSERDSVVQQAQAQF------HM----------------------AAEEARRNRLMRDMAQLRLQLEVSQLEGS--------------LQQPKAQSAMSPYLVLDAQALCQHLAVVKQLAGSGRFIIIIPRAVIDSLDFLKKENAGARDSIRYLEAEFKKGNRYIRCQ 947
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Cavefish
Match: smg5 (SMG5 nonsense mediated mRNA decay factor [Source:NCBI gene;Acc:103035711])

HSP 1 Score: 170.244 bits (430), Expect = 1.473e-42
Identity = 132/453 (29.14%), Postives = 229/453 (50.55%), Query Frame = 1 
Query:  130 KTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFV-PCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDM----NHYDIKQLAAMSDDVLNILSFVLGFDRKLVSNLSST------DNGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLK-FDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLLFHRNENKEEISVIKETCDEISSSKSTV 1452
            K L R V E++ KL  +   K++   +   E  +LR KL+E   +L+FL P+++G+KAE+ +W+ ++YE I++ K   K+    I+S+S +  A++ H++  +G+YQ L+L +Q+ ++L  + C D+T+   P +  ++    +P+   WA       L+Y+GDL RY++++    +  LA R+Y+Q LS++P +G  +NQL TL    +Y + A Y+YL+       F  A  NL  LF++  K    V  +E  MKK         L   R +S  IK L V  SF YL  +    N     +L ++   VL   + VL F +   S+ S +      D+    S L   +V +++ I   CL +           V  LK+   K +  + +F L L+ H+ N   +R++ EL     ++  L  R +N ++  V KE   ++ S + T+
Sbjct:   18 KRLYRAVVESVHKLDVIIGSKSSYREVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQVIKTNKKH----IHSRSALECAYRTHLIAGVGFYQHLLLYIQSHYQLELQDCIDWTHVTDPLIGRKKPVSATPKEMEWAQMACHRCLVYLGDLARYQNELAGVEAEQLAERFYHQALSVSPHVGMPFNQLGTLAGSKFYNVEATYYYLRCIQSETPFEGAYGNLKRLFDKAAKMYHQVKKQE--MKK---------LSPSRQRSKDIKRLLV--SFMYLQSLLQPKNSLMETELTSLCQSVLEDFNLVL-FYQPTPSHGSQSVSEEEDDHDQACSVLPDSLVFKMVVI---CLMV-----------VHSLKRGGSKQYSASIAFTLALFSHLVNHVNIRLQAELEDGESEVPPL--RTDNIDDADV-KEQAGKLPSEERTL 435

HSP 2 Score: 64.6994 bits (156), Expect = 5.445e-10
Identity = 40/139 (28.78%), Postives = 67/139 (48.20%), Query Frame = 1 
Query: 2590 KEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIESTAT-------LNNQDLACEHI 2985
            + ++MR+MA+LRL+ E+S    S      +     + S SP        YL+PD   +C  L+ ++ L     +I +IP+ VI  LD +K  + GAR  I++ E E    N +I+ +  +        L  QD+   H+
Sbjct:  898 RNRLMRDMAQLRLQLEVSQLEGS------LQQPKAQSSMSP--------YLVPDTSALCQHLNLLRQLAASGCFIIIIPRTVIDGLDVLKKENPGARDGIRFLESEFRKGNRYIRCQKESGRSFERDKLKRQDIEAWHL 1022
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Cavefish
Match: smg5 (SMG5 nonsense mediated mRNA decay factor [Source:NCBI gene;Acc:103035711])

HSP 1 Score: 170.244 bits (430), Expect = 1.554e-42
Identity = 132/453 (29.14%), Postives = 229/453 (50.55%), Query Frame = 1 
Query:  130 KTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFV-PCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDM----NHYDIKQLAAMSDDVLNILSFVLGFDRKLVSNLSST------DNGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLK-FDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLLFHRNENKEEISVIKETCDEISSSKSTV 1452
            K L R V E++ KL  +   K++   +   E  +LR KL+E   +L+FL P+++G+KAE+ +W+ ++YE I++ K   K+    I+S+S +  A++ H++  +G+YQ L+L +Q+ ++L  + C D+T+   P +  ++    +P+   WA       L+Y+GDL RY++++    +  LA R+Y+Q LS++P +G  +NQL TL    +Y + A Y+YL+       F  A  NL  LF++  K    V  +E  MKK         L   R +S  IK L V  SF YL  +    N     +L ++   VL   + VL F +   S+ S +      D+    S L   +V +++ I   CL +           V  LK+   K +  + +F L L+ H+ N   +R++ EL     ++  L  R +N ++  V KE   ++ S + T+
Sbjct:   18 KRLYRAVVESVHKLDVIIGSKSSYREVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQVIKTNKKH----IHSRSALECAYRTHLIAGVGFYQHLLLYIQSHYQLELQDCIDWTHVTDPLIGRKKPVSATPKEMEWAQMACHRCLVYLGDLARYQNELAGVEAEQLAERFYHQALSVSPHVGMPFNQLGTLAGSKFYNVEATYYYLRCIQSETPFEGAYGNLKRLFDKAAKMYHQVKKQE--MKK---------LSPSRQRSKDIKRLLV--SFMYLQSLLQPKNSLMETELTSLCQSVLEDFNLVL-FYQPTPSHGSQSVSEEEDDHDQACSVLPDSLVFKMVVI---CLMV-----------VHSLKRGGSKQYSASIAFTLALFSHLVNHVNIRLQAELEDGESEVPPL--RTDNIDDADV-KEQAGKLPSEERTL 435

HSP 2 Score: 64.6994 bits (156), Expect = 4.954e-10
Identity = 40/139 (28.78%), Postives = 67/139 (48.20%), Query Frame = 1 
Query: 2590 KEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIESTAT-------LNNQDLACEHI 2985
            + ++MR+MA+LRL+ E+S    S      +     + S SP        YL+PD   +C  L+ ++ L     +I +IP+ VI  LD +K  + GAR  I++ E E    N +I+ +  +        L  QD+   H+
Sbjct:  894 RNRLMRDMAQLRLQLEVSQLEGS------LQQPKAQSSMSP--------YLVPDTSALCQHLNLLRQLAASGCFIIIIPRTVIDGLDVLKKENPGARDGIRFLESEFRKGNRYIRCQKESGRSFERDKLKRQDIEAWHL 1018
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Cavefish
Match: smg6 (SMG6 nonsense mediated mRNA decay factor [Source:ZFIN;Acc:ZDB-GENE-081107-52])

HSP 1 Score: 69.707 bits (169), Expect = 1.635e-11
Identity = 63/257 (24.51%), Postives = 117/257 (45.53%), Query Frame = 1 
Query:  232 LRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNK-----PNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVW 981
            LR +L     R+I  + +EF   Q  +  +WK++FY+ I  F++ LK++     P L   +++++   +   +    ++  L+ +LQT F+   E   D+ + +   +    K +      +A    Q  ++  GD+ RY+     + +   A  +Y +   + P  G  YNQLA L   +   L+A Y+Y+++   SN    AKE+L+ LFE   KR    ++R       GS     G    R + +  +   +W
Sbjct:  579 LRAELLTLYERVILTD-IEFSDSQNLDQALWKNVFYQVIERFRQLLKDQDSDTAPQL---RAMLLTILEEGAM----FFDTLLQKLQTIFQFKLE---DYMDGMAIRARPLRKTVK-----YALISAQRCMICQGDIARYREQASDSANYGKARSWYLKAQQIAPKNGRPYNQLALLAVYTKRKLDAVYYYMRSLAASNPILTAKESLMSLFE-EAKRKAEQIERRRKQDSEGSVCGPRGRGWSRGEDATRQ--EIW 816
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Cavefish
Match: smg7 (SMG7 nonsense mediated mRNA decay factor [Source:NCBI gene;Acc:103037658])

HSP 1 Score: 58.9214 bits (141), Expect = 3.474e-8
Identity = 56/215 (26.05%), Postives = 97/215 (45.12%), Query Frame = 1 
Query:  208 LSTFEIQALRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFEL-LPEMCPDFTNFVPCVSTEEEKE---ISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENL 834
            L   E+   R  L++   +++  + LE+   +K E  +W   F   I   + + KN+ N   ++S +       +  A G+Y +L+  L T F + LP  C   ++ +  +S ++      + P+  S ++ C QH L+++GD+ RY+      N    A  YY     L PS G  YNQLA L +     L   ++Y ++      F  A  NL
Sbjct:   23 LGAAEVWTSRQALQDLYQKMLVTD-LEYALDKKVEQDLWNHAFKNQITSLQSQAKNRAN--PNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLP--CRVKSSQLGIISNKQSSTSAIVKPQPSSCSYIC-QHCLVHLGDIARYR------NQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNL 225
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Cavefish
Match: smg7 (SMG7 nonsense mediated mRNA decay factor [Source:NCBI gene;Acc:103037658])

HSP 1 Score: 58.5362 bits (140), Expect = 3.856e-8
Identity = 56/215 (26.05%), Postives = 97/215 (45.12%), Query Frame = 1 
Query:  208 LSTFEIQALRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFEL-LPEMCPDFTNFVPCVSTEEEKE---ISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENL 834
            L   E+   R  L++   +++  + LE+   +K E  +W   F   I   + + KN+ N   ++S +       +  A G+Y +L+  L T F + LP  C   ++ +  +S ++      + P+  S ++ C QH L+++GD+ RY+      N    A  YY     L PS G  YNQLA L +     L   ++Y ++      F  A  NL
Sbjct:   23 LGAAEVWTSRQALQDLYQKMLVTD-LEYALDKKVEQDLWNHAFKNQITSLQSQAKNRAN--PNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLP--CRVKSSQLGIISNKQSSTSAIVKPQPSSCSYIC-QHCLVHLGDIARYR------NQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNL 225
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Sea Lamprey
Match: smg5 (SMG5 nonsense mediated mRNA decay factor [Source:ZFIN;Acc:ZDB-GENE-050913-144])

HSP 1 Score: 141.739 bits (356), Expect = 2.041e-34
Identity = 109/416 (26.20%), Postives = 201/416 (48.32%), Query Frame = 1 
Query:  121 VKEKTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPCVST-EEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDMNH----YDIKQLAAMSDDVL---NILSFVLGFDRKLVSNLSSTDNGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLK-FDLAHSFALNLYGHVFNQCLVRIRDEL 1341
            V+ K L R V + + +L  +    +  + +   E  + R KL+E   +L+FL P+++G+KAE+ +W+ ++YE I   K   K     + ++  +  A++ H++  +G+YQ L+L LQ+ ++L  + C D+T+ V  +S  ++   +S     WA     H L+Y+GDL RY++++   ++  L+ R+Y Q L++ P +G   NQL TL     Y + AAY+Y++       F  A  N+  +F +  K    +L +E+  KK+          + + +S  +K L V  SF YL  + H        ++ ++   VL   N+  F L          +  + G  P   +   ++ LL      L +M          +  LK+   K F  A +F L L+ H+ N  ++R++ E+
Sbjct:   12 VQTKRLYRAVVDVVHRLDTIISSSSVCQEVFKPENVSQRNKLRELCVQLMFLHPVDYGRKAEELLWRKVYYEVIHAVKANRKQ----LQTRGSMECAYRTHLVAGVGFYQHLLLTLQSHYQLELQACIDWTHVVDTLSAVKKPAVVSSRALEWARMACHHCLIYLGDLARYQNELGVIDTHHLSERFYLQALAVAPHVGMPLNQLGTLAGNRSYNVEAAYYYMRCIQSEVGFDGAVGNMRRVFIKAKKMYMELLKKEAPCKKT---------SLSKQRSRDLKKLLV--SFLYLQSLLHPTSSVSEAEVTSLCQAVLHDFNLCMFYL--------PAAGANAGAGPEDHDAPCLSHLLVFRMLVLCLM---------GLHGLKRAGSKQFTTAVAFTLALFSHLLNHLIMRLQVEM 395

HSP 2 Score: 62.7734 bits (151), Expect = 4.173e-10
Identity = 74/294 (25.17%), Postives = 122/294 (41.50%), Query Frame = 1 
Query: 2104 LWKHFAVMLNFCENILDDLEQNDLTGIQCNHAT-------EKIRLLPPCYLPEDAELN-LLSVEHADKSIVLCENS-------LHQVVTRALRIIQCAHSIASISQGPIALQKINSSNLWLFSYSHNSPRTNLVFKSWTQIFSNSDSHLQGNPNSVAITTMSSSINAPSPAVVSAVDKEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIE 2940
            LW   +V+LN    IL D  +    G Q   A        E+    P   LPED  L  LL++  +   ++  E +       L Q V RA  +    H  A+++ G   L      +++L S S   P   +            D+   G  +++    M   +          + + ++MR+MA+LRL+ E+     S               RSP   A  + YL+PD   +   L  ++ L    R+I +IP+AVI  LD +K  +  AR AI++ EGE +  + +I+ +
Sbjct:  681 LWNRLSVLLN----ILPDSAKLMAAGTQLPPAAVRFVSGCERGEQRPAVALPEDVALRRLLALRESHARMLSSETAWQEPLSPLQQTVVRAACLRCMGHHFAALNTG--VLHYSTELSIFLGSSSAVEPEGGM----------GEDT---GVKDAMQQAEMQLKMAE------EELRRNRLMRDMAQLRLEEEVRELEGS--------------LRSPGTSASLSPYLVPDAGALSQHLQLVRQLAASARFILLIPRAVIDGLDVMKKDEATARDAIRFLEGEFKKGSRYIRCQ 935
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Nematostella
Match: EDO42311 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1W9])

HSP 1 Score: 119.013 bits (297), Expect = 4.382e-30
Identity = 76/225 (33.78%), Postives = 114/225 (50.67%), Query Frame = 1 
Query:  193 NAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPCVSTEEE-----KEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFER 852
            N EEI    E   +R +L+     LIF  P E+G+KAE+ +W+ +FY+ I+  ++  K  PN  + +S    + + H++ A GYY  L+++LQ+ +          TN    VS   +       I  E+  WA    Q  LLY+GDL RY+ + +   S  LA R+Y + L LNP LG  +NQL TL      G    Y YL+  +   SF   + NL+ LFE+
Sbjct:   20 NWEEIFQA-ETIIIRQRLRSNCESLIFNHPKEYGRKAEELLWRKVFYDVIQWLRQHRKVSPNDEHLES----SCRSHLISASGYYYHLLIQLQSLYG---------TNLKGVVSWTAQGLHTASGIDAEVADWALRACQRCLLYMGDLARYQQEFEGEKSIKLAERFYCEALHLNPQLGMPHNQLGTLLVSQSCGAEGVYHYLRCLIAKESFEGTEGNLVRLFEK 230
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Nematostella
Match: EDO34379 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SPJ8])

HSP 1 Score: 58.5362 bits (140), Expect = 1.061e-8
Identity = 60/225 (26.67%), Postives = 95/225 (42.22%), Query Frame = 1 
Query:  187 IKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFEL-LPEMCPDFTNFVPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFER 852
            ++ AE I +  + Q L+      L + I L  LE    +K E  +W S F   I   +++ ++K     SK+ +       +  A G+Y +L+  L   ++L LP         +            P  +S  + C Q  L+++GD+ RY  D   T + +    +Y   L L PS G  YNQLA L +     L   YFY ++    N F  A  NL   F +
Sbjct:   12 LRQAETIRAGLKTQRLQD-----LYKRILLNDLELALDKKIEQDLWNSAFKSQIDFLRQKARDKK--CTSKNEVQAVLTLFLETASGFYIQLLQELCMTYDLDLPGHARSSHLGLLDDGDVNSSSKRPHTQSCYYVC-QDCLVHLGDIARYCDD--ATQAEV----FYRHALVLVPSNGQPYNQLAILASARGDHLGTVYFYCRSIAVKNQFPAASTNLHKTFNK 222
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Medaka
Match: smg5 (SMG5 nonsense mediated mRNA decay factor [Source:NCBI gene;Acc:101173007])

HSP 1 Score: 157.532 bits (397), Expect = 1.455e-38
Identity = 119/436 (27.29%), Postives = 205/436 (47.02%), Query Frame = 1 
Query:  130 KTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFV-PCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDM----NHYDIKQLAAMSDDVLNILSFVLGF----------------DRKLVSNLSSTDNGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLK-FDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLL 1371
            K L R V E++ KL  +   K++   +   E  +LR KL+E   +L+FL P+++G+KAE+ +W+ ++YE I++ K   K+    ++S+S +  A++ H++  +G+YQ L+L +Q+ ++L  + C D+T+   P +  ++    SP+   WA       L+Y+GDL RY++++    +  LA R+Y+Q LS+ P +G  +NQL TL    +Y + A Y+YL+       F  A  NL  LF++  K    V  ++  MKK         L   R +S  +K L V  SF YL  +    N     +L ++   VL   + VL +                + +           V P  L  K+V   L +                  V  LK+   K +  + +F L L+ H+ N   +R++ EL      +  L
Sbjct:   18 KRLYRAVVESVHKLDVIIGSKSSYREVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQVIKTNKKH----VHSRSALECAYRTHLIAGVGFYQHLLLYVQSHYQLELQDCIDWTHVTDPLIGRKKPVSASPKEMEWAQMACHRCLVYLGDLARYQNELAGVETEQLAERFYHQALSVMPHVGMPFNQLGTLAGSKFYNVEATYYYLRCIQSDAPFEGAYGNLKRLFDKAAKMYHQV--KKPEMKK---------LSPSRKRSKDVKRLLV--SFMYLQSLLQPKNSLLETELTSLCQSVLEDFNLVLFYLPPPAHGGAQHSPSEEEEEQHQQHPDVSCPVLPDGLIFKMVVTCLMV------------------VHSLKRGGSKQYSASIAFTLALFSHLVNHVNIRLQAELEEGGGQVPAL 418

HSP 2 Score: 68.5514 bits (166), Expect = 3.150e-11
Identity = 43/139 (30.94%), Postives = 67/139 (48.20%), Query Frame = 1 
Query: 2590 KEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIESTAT-------LNNQDLACEHI 2985
            + ++MR+MA+LRL+ E+S    S      +     + S SP        YL+PD   +C  L  I+ L     +I +IP+AVI  LD +K  + GAR  I++ E E    N +I+ +  +        L  QDL   H+
Sbjct:  914 RNRLMRDMAQLRLQLEVSQLEGS------LQQPKAQSSMSP--------YLVPDTAALCQHLSLIRQLAGSGCFIIIIPRAVIDGLDALKKENAGARDGIRFLESEFRKGNRYIRCQKESGRSFERDKLKRQDLEAWHL 1038
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Medaka
Match: smg5 (SMG5 nonsense mediated mRNA decay factor [Source:NCBI gene;Acc:101173007])

HSP 1 Score: 157.147 bits (396), Expect = 1.942e-38
Identity = 119/436 (27.29%), Postives = 205/436 (47.02%), Query Frame = 1 
Query:  130 KTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFV-PCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDM----NHYDIKQLAAMSDDVLNILSFVLGF----------------DRKLVSNLSSTDNGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLK-FDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLL 1371
            K L R V E++ KL  +   K++   +   E  +LR KL+E   +L+FL P+++G+KAE+ +W+ ++YE I++ K   K+    ++S+S +  A++ H++  +G+YQ L+L +Q+ ++L  + C D+T+   P +  ++    SP+   WA       L+Y+GDL RY++++    +  LA R+Y+Q LS+ P +G  +NQL TL    +Y + A Y+YL+       F  A  NL  LF++  K    V  ++  MKK         L   R +S  +K L V  SF YL  +    N     +L ++   VL   + VL +                + +           V P  L  K+V   L +                  V  LK+   K +  + +F L L+ H+ N   +R++ EL      +  L
Sbjct:   18 KRLYRAVVESVHKLDVIIGSKSSYREVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQVIKTNKKH----VHSRSALECAYRTHLIAGVGFYQHLLLYVQSHYQLELQDCIDWTHVTDPLIGRKKPVSASPKEMEWAQMACHRCLVYLGDLARYQNELAGVETEQLAERFYHQALSVMPHVGMPFNQLGTLAGSKFYNVEATYYYLRCIQSDAPFEGAYGNLKRLFDKAAKMYHQV--KKPEMKK---------LSPSRKRSKDVKRLLV--SFMYLQSLLQPKNSLLETELTSLCQSVLEDFNLVLFYLPPPAHGGAQHSPSEEEEEQHQQHPDVSCPVLPDGLIFKMVVTCLMV------------------VHSLKRGGSKQYSASIAFTLALFSHLVNHVNIRLQAELEEGGGQVPAL 418

HSP 2 Score: 68.9366 bits (167), Expect = 2.838e-11
Identity = 43/139 (30.94%), Postives = 67/139 (48.20%), Query Frame = 1 
Query: 2590 KEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIESTAT-------LNNQDLACEHI 2985
            + ++MR+MA+LRL+ E+S    S      +     + S SP        YL+PD   +C  L  I+ L     +I +IP+AVI  LD +K  + GAR  I++ E E    N +I+ +  +        L  QDL   H+
Sbjct:  914 RNRLMRDMAQLRLQLEVSQLEGS------LQQPKAQSSMSP--------YLVPDTAALCQHLSLIRQLAGSGCFIIIIPRAVIDGLDALKKENAGARDGIRFLESEFRKGNRYIRCQKESGRSFERDKLKRQDLEAWHL 1038
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Medaka
Match: smg6 (SMG6 nonsense mediated mRNA decay factor [Source:NCBI gene;Acc:101166408])

HSP 1 Score: 67.3958 bits (163), Expect = 8.846e-11
Identity = 62/259 (23.94%), Postives = 116/259 (44.79%), Query Frame = 1 
Query:  142 RVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGS 912
            RV A+ L ++ QL       ++L  +E               + L  +EF   Q  +  +WK++FY+ I  F++ LK+ P+  N+  +  +       GA  ++  L+ ++QT ++   E   D+ + +   +    K +      +A    Q  ++  GD+ RY+     + +   A  +Y +   + P  G  YNQLA L   +   L+A Y+Y+++   SN    AKE+L+ LFE   ++   +  R     + GS
Sbjct:  794 RVSADGLDRMAQLR-----ADLLGLYE--------------QVILTDIEFSDSQNVDQALWKNVFYQVIERFRQLLKD-PSYDNTPHIRNMLLTLLDEGAQ-FFDALLQKVQTVYQFTLE---DYMDGMAIRARPLRKTVK-----YALISAQRCMICQGDIARYREQATDSANYGKARSWYLKAQQIAPKNGRPYNQLALLAIYTKRKLDAVYYYMRSLAASNPILTAKESLMSLFEEAKRKAEQLEQRRRQENEGGS 1023
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Medaka
Match: smg7 (SMG7 nonsense mediated mRNA decay factor [Source:NCBI gene;Acc:101155720])

HSP 1 Score: 60.077 bits (144), Expect = 1.458e-8
Identity = 56/215 (26.05%), Postives = 98/215 (45.58%), Query Frame = 1 
Query:  208 LSTFEIQALRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFEL-LPEMCPDFTNFVPCVSTEEEKE---ISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENL 834
            L   E+   R  L++   +++  + LE+   +K E  +W   F   I   + + KN+ N   ++S +       +  A G+Y +L+  L T F + LP  C   ++ +  +S ++      ++P+  S ++ C QH L+++GD+ RY+      N    A  YY     L PS G  YNQLA L +     L   ++Y ++      F  A  NL
Sbjct:   23 LGAAEVWTSRQALQDLYQKMLVTD-LEYALDKKVEQDLWNHAFKNQITTLQSQAKNRAN--PNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLP--CRVRSSQLGIISNKQGSGGAIVTPQPSSCSYIC-QHCLVHLGDIARYR------NQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNL 225
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Medaka
Match: smg7 (SMG7 nonsense mediated mRNA decay factor [Source:NCBI gene;Acc:101155720])

HSP 1 Score: 60.077 bits (144), Expect = 1.516e-8
Identity = 56/215 (26.05%), Postives = 98/215 (45.58%), Query Frame = 1 
Query:  208 LSTFEIQALRYKLKECLNRLIFLEPLEFG--QKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFEL-LPEMCPDFTNFVPCVSTEEEKE---ISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENL 834
            L   E+   R  L++   +++  + LE+   +K E  +W   F   I   + + KN+ N   ++S +       +  A G+Y +L+  L T F + LP  C   ++ +  +S ++      ++P+  S ++ C QH L+++GD+ RY+      N    A  YY     L PS G  YNQLA L +     L   ++Y ++      F  A  NL
Sbjct:   23 LGAAEVWTSRQALQDLYQKMLVTD-LEYALDKKVEQDLWNHAFKNQITTLQSQAKNRAN--PNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLP--CRVRSSQLGIISNKQGSGGAIVTPQPSSCSYIC-QHCLVHLGDIARYR------NQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNL 225
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Planmine SMEST
Match: SMESG000046002.1 (SMESG000046002.1)

HSP 1 Score: 2051.17 bits (5313), Expect = 0.000e+0
Identity = 1007/1049 (96.00%), Postives = 1009/1049 (96.19%), Query Frame = 1 
Query:   70 MDSKAQNLRIRIRQNASVKEKTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDMNHYDIKQLAAMSDDVLNILSFVLGFDRKLVSNLSSTDNGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLKFDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLLFHRNENKEEISVIKETCDEISSSKSTVAISPDHSVVKSRKKRVGLSKRLVRHRHDTDEASIEEDLSEDDQNLSENDNILWDDQISDSSDSNVGDEGGFGFSDVEDETDFESNEEIHSQDEDDQIIRAIVGHNTIFGRNQSLNGKDLKRSRDKESANNSLVNKVIFSETLIHESSQSIKPPKRRSATSQGVFNQFNNVKPSAEALVTCIFNVDLLQSTSVVISAFNHFINDKLESFCRVWQYASYLWKHFAVMLNFCENILDDLEQNDLTGIQCNHATEKIRLLPPCYLPEDAELNLLSVEHADKSIVLCENSLHQVVTRALRIIQCAHSIASISQGPIALQKINSSNLWLFSYSHNSPRTNLVFKSWTQIFSNSDSHLQGNPNSVAITTMSSSINAPSPAVVSAVDKEKVMREMARLRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIESTATLNNQDLACEHILDWPAIETSATRLVEESFLKSALTHEVVVTILTNSASKLSSQFMEYYSNKRSIFLKIVYKAVNFFLISVNFQSTIDL 3216
            MDSKAQNLRIRIRQNASVKEKTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDMNHYDIKQLAAMSDDVLNILSFVLGFDRKLVSNLSSTDNGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLKFDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLLFHRNENKEEISVIKETCDEI+SSKSTVAISPDHSVVKSRKKRVGLSKRLVRHRHDTDEASIEEDLSEDDQNLSENDNILWDDQISDSSDSNVGDEGGFGFSDVEDETDFESNEEIHSQDEDDQIIRAIVGHNTIFGRNQSLNGKDLKRSRDKESANNSLVNKVIFSETLIHESSQSIKPPKRRSATSQGVFNQFNNVKPSAEALVTCIFNVDLLQSTSVVISAFNHFINDKLESFCRVWQYA YLWKHFAVMLNFCENILDDLEQNDLTGIQCNHATEKIRLLPPCYLPEDAELNLLSVEHADKSIV CEN LHQVVTRALRIIQCAHSIASISQGPIALQKINSSNLWLFSYSHNSPRTNLVFKSWTQIFSNSDSHLQGNPNSVAITTMSSSINAPSPAVVSAVDKEKVMREMARLRLKNEL                    SRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIESTATLNNQDLACEHILDWPAIETSATRLVEESFLKSALTHEVVVTILTNSASKLSSQFMEYYSNKR                 +NFQSTIDL
Sbjct:    1 MDSKAQNLRIRIRQNASVKEKTLIRVVAETLKKLVQLYQIKNAEEILSTFEIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNFVPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKYLNVWYSFAYLNDMNHYDIKQLAAMSDDVLNILSFVLGFDRKLVSNLSSTDNGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLKFDLAHSFALNLYGHVFNQCLVRIRDELRTSNIDISLLFHRNENKEEISVIKETCDEIASSKSTVAISPDHSVVKSRKKRVGLSKRLVRHRHDTDEASIEEDLSEDDQNLSENDNILWDDQISDSSDSNVGDEGGFGFSDVEDETDFESNEEIHSQDEDDQIIRAIVGHNTIFGRNQSLNGKDLKRSRDKESANNSLVNKVIFSETLIHESSQSIKPPKRRSATSQGVFNQFNNVKPSAEALVTCIFNVDLLQSTSVVISAFNHFINDKLESFCRVWQYAPYLWKHFAVMLNFCENILDDLEQNDLTGIQCNHATEKIRLLPPCYLPEDAELNLLSVEHADKSIVHCENPLHQVVTRALRIIQCAHSIASISQGPIALQKINSSNLWLFSYSHNSPRTNLVFKSWTQIFSNSDSHLQGNPNSVAITTMSSSINAPSPAVVSAVDKEKVMREMARLRLKNEL--------------------SRSPSNDAIRAVYLIPDVEYICNRLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELNNEHIKIESTATLNNQDLACEHILDWPAIETSATRLVEESFLKSALTHEVVVTILTNSASKLSSQFMEYYSNKR-----------------INFQSTIDL 1012
[Close]
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Planmine SMEST
Match: SMESG000051442.1 (SMESG000051442.1)

HSP 1 Score: 57.7658 bits (138), Expect = 5.816e-8
Identity = 56/221 (25.34%), Postives = 93/221 (42.08%), Query Frame = 1 
Query:  223 IQALRYKLKECLNRLIFLEPLEFGQKA--EDTIWKSIFYEPIRIFKRELKNKPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNF------VPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILATRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQAKENLLILFERNVK 861
            + ++R +L + L R +    L++  +A  E  +WKS+FY  + +  R   N P+ I           +  +   G+ Q +   LQ+  E    +  D +        V   S     E     +   +   Q  LL++GDL RY   +  ++    A  +Y +   L P  G  YNQLA +   +   L+A Y+Y +    SN F  A ++L  LFE   K
Sbjct:  439 VNSVRSELMD-LYRSVITSDLDYCNQAGIEQNLWKSVFYSIMELI-RAFINNPDTIALPQNQKDQVSKIKMSVTGHGQNI---LQSGEEYFISIFTDISKLHNIDLEVIFDSRRPPPETGSRTRKLVYLSAQKLLLFLGDLARYSEMINNSSDFTKARIWYLKAQLLVPKNGRPYNQLAVISIYANRHLDALYYYTRTLAASNPFPTASQSLSFLFEETRK 654
The following BLAST results are available for this feature:
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SMG56.712e-3726.78SMG5 nonsense mediated mRNA decay factor [Source:H... [more]
SMG64.056e-1124.83SMG6 nonsense mediated mRNA decay factor [Source:H... [more]
SMG75.051e-825.11SMG7 nonsense mediated mRNA decay factor [Source:H... [more]
SMG76.388e-825.11SMG7 nonsense mediated mRNA decay factor [Source:H... [more]
SMG76.731e-825.11SMG7 nonsense mediated mRNA decay factor [Source:H... [more]
back to top
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Smg53.088e-1326.18gene:FBgn0019890 transcript:FBtr0343669[more]
Smg53.088e-1326.18gene:FBgn0019890 transcript:FBtr0080562[more]
Smg53.088e-1326.18gene:FBgn0019890 transcript:FBtr0080561[more]
Smg63.037e-934.45gene:FBgn0039260 transcript:FBtr0084796[more]
back to top
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
smg52.228e-4028.70SMG5 nonsense mediated mRNA decay factor [Source:Z... [more]
smg63.481e-1427.04SMG6 nonsense mediated mRNA decay factor [Source:Z... [more]
smg71.030e-826.05SMG7 nonsense mediated mRNA decay factor [Source:Z... [more]
smg71.154e-826.05SMG7 nonsense mediated mRNA decay factor [Source:Z... [more]
back to top
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
smg53.366e-1230.77SMG5 nonsense mediated mRNA decay factor [Source:N... [more]
smg53.867e-1231.30SMG5 nonsense mediated mRNA decay factor [Source:N... [more]
smg54.140e-1230.77SMG5 nonsense mediated mRNA decay factor [Source:N... [more]
smg54.678e-1230.77SMG5 nonsense mediated mRNA decay factor [Source:N... [more]
mgme11.395e-924.90mitochondrial genome maintenance exonuclease 1 [So... [more]
back to top
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Smg51.826e-4127.36Smg-5 homolog, nonsense mediated mRNA decay factor... [more]
Smg51.237e-3334.43Smg-5 homolog, nonsense mediated mRNA decay factor... [more]
Smg69.095e-1326.80Smg-6 homolog, nonsense mediated mRNA decay factor... [more]
Smg61.213e-1226.67Smg-6 homolog, nonsense mediated mRNA decay factor... [more]
Smg73.078e-825.11Smg-7 homolog, nonsense mediated mRNA decay factor... [more]
back to top
BLAST of SMG5 nonsense mediated mRNA decay factor vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6ZPY2|SMG5_MOUSE1.278e-4027.36Protein SMG5 OS=Mus musculus OX=10090 GN=Smg5 PE=1... [more]
sp|Q9UPR3|SMG5_HUMAN3.223e-3626.78Protein SMG5 OS=Homo sapiens OX=9606 GN=SMG5 PE=1 ... [more]
sp|P61406|EST1A_MOUSE8.484e-1226.67Telomerase-binding protein EST1A OS=Mus musculus O... [more]
sp|Q5RAK6|EST1A_PONAB7.871e-1124.83Telomerase-binding protein EST1A OS=Pongo abelii O... [more]
sp|Q86US8|EST1A_HUMAN1.948e-1024.83Telomerase-binding protein EST1A OS=Homo sapiens O... [more]
back to top
BLAST of SMG5 nonsense mediated mRNA decay factor vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A151NY353.484e-5024.30Protein SMG5 OS=Alligator mississippiensis OX=8496... [more]
H0YZZ84.286e-4924.58PINc domain-containing protein OS=Taeniopygia gutt... [more]
A0A3P9CGU14.306e-4924.33SMG5 nonsense mediated mRNA decay factor OS=Maylan... [more]
A0A1D5PSK61.088e-4723.40PINc domain-containing protein OS=Gallus gallus OX... [more]
A0A1D5PME44.231e-4723.27PINc domain-containing protein OS=Gallus gallus OX... [more]
back to top
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
smg51.473e-4229.14SMG5 nonsense mediated mRNA decay factor [Source:N... [more]
smg51.554e-4229.14SMG5 nonsense mediated mRNA decay factor [Source:N... [more]
smg61.635e-1124.51SMG6 nonsense mediated mRNA decay factor [Source:Z... [more]
smg73.474e-826.05SMG7 nonsense mediated mRNA decay factor [Source:N... [more]
smg73.856e-826.05SMG7 nonsense mediated mRNA decay factor [Source:N... [more]
back to top
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
smg52.041e-3426.20SMG5 nonsense mediated mRNA decay factor [Source:Z... [more]
back to top
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO423114.382e-3033.78Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO343791.061e-826.67Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
smg51.455e-3827.29SMG5 nonsense mediated mRNA decay factor [Source:N... [more]
smg51.942e-3827.29SMG5 nonsense mediated mRNA decay factor [Source:N... [more]
smg68.846e-1123.94SMG6 nonsense mediated mRNA decay factor [Source:N... [more]
smg71.458e-826.05SMG7 nonsense mediated mRNA decay factor [Source:N... [more]
smg71.516e-826.05SMG7 nonsense mediated mRNA decay factor [Source:N... [more]
back to top
BLAST of SMG5 nonsense mediated mRNA decay factor vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000046002.10.000e+096.00SMESG000046002.1[more]
SMESG000051442.15.816e-825.34SMESG000051442.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001753 ID=SMED30001753|Name=SMG5 nonsense mediated mRNA decay factor|organism=Schmidtea mediterranea sexual|type=transcript|length=3299bp
ATTACTTATAAAGTAACAATATTTAAATTTCTTGATAATTGTTTAAAAAT
ATTTATTCTTTAACTATTTATGGATTCGAAAGCCCAAAATCTGAGAATTC
GTATAAGGCAAAATGCTAGCGTTAAAGAAAAGACTCTTATTAGAGTTGTT
GCAGAAACCCTAAAAAAGTTAGTTCAGCTATATCAAATTAAAAATGCAGA
AGAAATATTATCGACTTTTGAAATTCAAGCTTTAAGATATAAACTCAAAG
AATGTCTAAATCGACTTATATTTCTAGAACCACTTGAATTTGGTCAAAAG
GCTGAAGATACCATATGGAAGTCTATTTTCTATGAACCCATAAGGATTTT
TAAACGAGAATTGAAAAATAAACCAAATTTAATTAATTCTAAATCATTAA
TAATAGTGGCATTTCAACGACATGTTCTAGGGGCAATTGGTTATTATCAA
AAATTGATTCTTCGGTTACAAACCGAATTCGAACTCCTACCAGAAATGTG
CCCAGATTTTACAAACTTTGTCCCGTGTGTTTCTACCGAAGAAGAGAAAG
AAATTTCCCCGGAACTTAAGTCATGGGCATTCAGTTGTATTCAACATAGC
TTGTTGTATATTGGGGATTTATATCGCTATAAGCACGACATGAAGTGTAC
AAATTCACGCATTCTAGCAACGAGATATTATAACCAAGTGCTTTCACTGA
ATCCGTCTTTAGGCCATGCTTATAATCAGCTGGCCACGCTTGATACGTTA
AGTTATTATGGGTTAAATGCTGCTTATTTCTATCTAAAAGCACAATTATG
TTCTAATTCCTTTTCTCAAGCAAAAGAAAATTTATTAATACTTTTTGAAA
GAAATGTCAAGCGTTTGCGAAATGTATTAGATCGTGAGTCGATGATGAAA
AAATCCGGTTCTAATATAACAAAAAGTGGACTAAAAATAATGAGAGTCAA
ATCGAGCATTATCAAATATTTGAATGTTTGGTATTCATTTGCATATTTAA
ATGACATGAATCACTATGATATCAAACAATTGGCAGCTATGAGTGATGAT
GTTCTTAACATATTGAGTTTTGTTTTGGGGTTCGATAGAAAACTCGTCTC
AAATCTATCAAGCACTGATAACGGTGTTCGTCCATCGACTCTCAATGTCA
AAATTGTTACTCAACTGCTTTTCATTGCGGATTCTTGTTTGAATATAATG
CTACCAAAAAAAGAAGATCAAGAGACTGATGTTGAGAAGCTGAAACAAGT
ATCATTAAAGTTTGATCTAGCTCATTCTTTTGCATTGAATTTATACGGCC
ACGTATTCAATCAATGTCTCGTTCGTATTAGAGATGAATTACGCACATCT
AATATAGACATATCGCTCTTATTTCACCGTAATGAAAATAAAGAAGAAAT
TTCAGTTATTAAAGAGACATGCGATGAAATTTCTTCTAGTAAATCCACAG
TTGCAATTTCACCTGACCATAGTGTAGTTAAATCTCGTAAAAAGCGAGTA
GGTCTGTCGAAAAGATTGGTTAGACATCGACACGATACTGACGAAGCAAG
CATTGAAGAAGATCTTAGTGAAGATGATCAAAATTTATCAGAAAATGACA
ACATTTTATGGGATGATCAGATTTCCGATAGTTCTGATAGTAATGTGGGC
GATGAAGGTGGATTTGGATTTAGTGATGTTGAAGATGAGACAGATTTTGA
ATCCAATGAGGAAATTCATTCCCAGGACGAAGATGACCAAATAATACGAG
CTATTGTAGGACATAACACTATATTTGGACGGAATCAAAGTCTCAATGGA
AAGGATCTGAAGAGGTCGAGAGATAAGGAATCAGCAAATAACAGTCTTGT
TAACAAAGTGATATTTAGTGAAACGCTTATTCACGAATCAAGTCAAAGTA
TCAAACCACCAAAGCGCCGTTCAGCTACGTCACAGGGAGTTTTTAATCAG
TTCAATAACGTTAAGCCATCCGCAGAGGCATTGGTGACTTGTATATTTAA
TGTTGATTTGCTTCAATCAACATCCGTCGTTATTTCTGCTTTCAATCATT
TCATTAATGATAAGTTGGAGAGTTTCTGCCGAGTTTGGCAATATGCGTCC
TATCTTTGGAAGCATTTTGCAGTAATGCTAAACTTCTGTGAGAATATTCT
CGATGATTTAGAGCAAAATGACCTCACTGGCATACAGTGTAATCATGCAA
CCGAGAAAATTCGCTTGCTGCCACCTTGTTATCTACCAGAAGATGCTGAA
TTGAATCTTTTGTCTGTGGAACATGCCGATAAGAGCATTGTGCTTTGTGA
AAATTCCCTTCATCAAGTCGTTACTAGAGCCCTACGGATTATTCAATGTG
CTCATTCGATTGCTTCAATATCCCAGGGACCGATTGCTTTACAGAAAATT
AATTCCAGCAATCTGTGGCTGTTTTCTTATTCTCACAATTCTCCAAGGAC
GAATTTAGTTTTTAAATCCTGGACTCAAATATTCTCGAATTCTGACAGCC
ATTTACAAGGAAATCCAAATTCCGTAGCAATCACCACAATGAGCAGTTCA
ATCAACGCACCGTCACCTGCAGTCGTATCTGCGGTCGACAAAGAAAAGGT
GATGCGCGAAATGGCAAGATTGCGACTGAAAAATGAGTTGTCAAGTTCAG
CAGAATCAGACGACAATAGAGATCTAGTAAGTAACAATCACAAGAAAAGA
TCGAGAAGCCCATCCAACGATGCAATCCGTGCTGTTTATTTGATCCCAGA
TGTTGAATATATTTGTAACCGACTTGATACCATTAAAAGTCTGATTAGAT
GTCAAAGATATATATTTGTAATTCCTCAAGCTGTCATTCAGCGCTTGGAT
CGAATTAAAAGTCACGATCACGGTGCAAGGCAGGCAATTAAATATTTTGA
AGGAGAAATGGAGTTAAATAACGAACATATCAAGATTGAATCGACTGCTA
CATTGAATAATCAAGATTTGGCGTGTGAACATATTTTAGATTGGCCCGCA
ATAGAAACAAGTGCAACAAGATTGGTTGAAGAATCTTTTCTGAAATCGGC
TCTTACGCACGAGGTTGTTGTTACAATTCTCACAAATTCAGCATCAAAGC
TCTCCAGCCAATTCATGGAATACTATTCTAACAAGCGAAGCATTTTTTTG
AAAATTGTTTATAAAGCTGTTAATTTTTTTTTAATTTCAGTTAATTTCCA
GTCGACTATTGATCTCTGAGTACGTTTAATGGAATTGGCCTGTATTTTGT
TGTTCAAATTATCAAGTTATTTGTAAAATATACGCCATCTAGATCGGAA
back to top

protein sequence of SMED30001753-orf-1

>SMED30001753-orf-1 ID=SMED30001753-orf-1|Name=SMED30001753-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1050bp
MDSKAQNLRIRIRQNASVKEKTLIRVVAETLKKLVQLYQIKNAEEILSTF
EIQALRYKLKECLNRLIFLEPLEFGQKAEDTIWKSIFYEPIRIFKRELKN
KPNLINSKSLIIVAFQRHVLGAIGYYQKLILRLQTEFELLPEMCPDFTNF
VPCVSTEEEKEISPELKSWAFSCIQHSLLYIGDLYRYKHDMKCTNSRILA
TRYYNQVLSLNPSLGHAYNQLATLDTLSYYGLNAAYFYLKAQLCSNSFSQ
AKENLLILFERNVKRLRNVLDRESMMKKSGSNITKSGLKIMRVKSSIIKY
LNVWYSFAYLNDMNHYDIKQLAAMSDDVLNILSFVLGFDRKLVSNLSSTD
NGVRPSTLNVKIVTQLLFIADSCLNIMLPKKEDQETDVEKLKQVSLKFDL
AHSFALNLYGHVFNQCLVRIRDELRTSNIDISLLFHRNENKEEISVIKET
CDEISSSKSTVAISPDHSVVKSRKKRVGLSKRLVRHRHDTDEASIEEDLS
EDDQNLSENDNILWDDQISDSSDSNVGDEGGFGFSDVEDETDFESNEEIH
SQDEDDQIIRAIVGHNTIFGRNQSLNGKDLKRSRDKESANNSLVNKVIFS
ETLIHESSQSIKPPKRRSATSQGVFNQFNNVKPSAEALVTCIFNVDLLQS
TSVVISAFNHFINDKLESFCRVWQYASYLWKHFAVMLNFCENILDDLEQN
DLTGIQCNHATEKIRLLPPCYLPEDAELNLLSVEHADKSIVLCENSLHQV
VTRALRIIQCAHSIASISQGPIALQKINSSNLWLFSYSHNSPRTNLVFKS
WTQIFSNSDSHLQGNPNSVAITTMSSSINAPSPAVVSAVDKEKVMREMAR
LRLKNELSSSAESDDNRDLVSNNHKKRSRSPSNDAIRAVYLIPDVEYICN
RLDTIKSLIRCQRYIFVIPQAVIQRLDRIKSHDHGARQAIKYFEGEMELN
NEHIKIESTATLNNQDLACEHILDWPAIETSATRLVEESFLKSALTHEVV
VTILTNSASKLSSQFMEYYSNKRSIFLKIVYKAVNFFLISVNFQSTIDL*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR018834DNA/RNA-bd_Est1-type
IPR019458EST1
IPR011990TPR-like_helical_dom_sf
IPR001148CA_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0004540ribonuclease activity
GO:0031625ubiquitin protein ligase binding
GO:0042162telomeric DNA binding
GO:0042826histone deacetylase binding
GO:0043021ribonucleoprotein complex binding
GO:0051721protein phosphatase 2A binding
GO:0070034telomerase RNA binding
GO:0070182DNA polymerase binding
Vocabulary: biological process
TermDefinition
GO:0000184nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
GO:0006406mRNA export from nucleus
GO:0007004telomere maintenance via telomerase
GO:0032204regulation of telomere maintenance
GO:0035303regulation of dephosphorylation
GO:0043487regulation of RNA stability
GO:0090501RNA phosphodiester bond hydrolysis
Vocabulary: cellular component
TermDefinition
GO:0000932P-body
GO:0005634nucleus
GO:0005697telomerase holoenzyme complex
GO:0005737cytoplasm