Calcyphosin-like protein

Overview
NameCalcyphosin-like protein
Smed IDSMED30001720
Length (bp)846
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Calcyphosin-like protein (SMED30001720) t-SNE clustered cells

Violin plots show distribution of expression levels for Calcyphosin-like protein (SMED30001720) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Calcyphosin-like protein (SMED30001720) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Calcyphosin-like protein (SMED30001720) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30001720

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30001720h1SMcG0013152 Contig38716uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001720h1SMcG0013152 Contig38716newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30001720h1SMcG0013152 dd_Smed_v4_1554_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30001720h1SMcG0013152 dd_Smed_v4_1554_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30001720h1SMcG0013152 dd_Smed_v4_1554_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30001720h1SMcG0013152 dd_Smed_v4_1554_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30001720h1SMcG0013152 dd_Smed_v4_1554_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30001720h1SMcG0013152 dd_Smed_v4_1554_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30001720h1SMcG0013152 dd_Smed_v4_1554_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30001720h1SMcG0013152 dd_Smed_v4_1554_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
secretory systemSMED30001720h1SMcG0013152 dd_Smed_v6_1554_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Calcyphosin-like protein vs. Ensembl Human
Match: CAPSL (calcyphosine like [Source:HGNC Symbol;Acc:HGNC:28375])

HSP 1 Score: 225.713 bits (574), Expect = 1.380e-73
Identity = 109/208 (52.40%), Postives = 156/208 (75.00%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MA + + D E+ ++A+K+L+TA DP+ +LRLQCL  G++GIKGL R FRIM+DDN+R L   EF  G+  + +++   E+E+LF+  D+DG+ TI   EFL  LRPP+S  R ++I +AF  +DKTGDGVITIEDL+ VYNA  HPK QNGEWSE +VF++FL +F++P D DG+VT +EF++YY+ +S SID + YF +MMR ++K+
Sbjct:    1 MAGTARHDREMAIQAKKKLTTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDDNNRTLDFKEFMKGLNDYAVVMEKEEVEELFRRFDKDGNGTIDFNEFLLTLRPPMSRARKEVIMQAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWSEEQVFRKFLDNFDSPYDKDGLVTPEEFMNYYAGVSASIDTDVYFIIMMRTAWKL 208          
BLAST of Calcyphosin-like protein vs. Ensembl Human
Match: CAPSL (calcyphosine like [Source:HGNC Symbol;Acc:HGNC:28375])

HSP 1 Score: 225.713 bits (574), Expect = 1.380e-73
Identity = 109/208 (52.40%), Postives = 156/208 (75.00%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MA + + D E+ ++A+K+L+TA DP+ +LRLQCL  G++GIKGL R FRIM+DDN+R L   EF  G+  + +++   E+E+LF+  D+DG+ TI   EFL  LRPP+S  R ++I +AF  +DKTGDGVITIEDL+ VYNA  HPK QNGEWSE +VF++FL +F++P D DG+VT +EF++YY+ +S SID + YF +MMR ++K+
Sbjct:    1 MAGTARHDREMAIQAKKKLTTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDDNNRTLDFKEFMKGLNDYAVVMEKEEVEELFRRFDKDGNGTIDFNEFLLTLRPPMSRARKEVIMQAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWSEEQVFRKFLDNFDSPYDKDGLVTPEEFMNYYAGVSASIDTDVYFIIMMRTAWKL 208          
BLAST of Calcyphosin-like protein vs. Ensembl Human
Match: CAPSL (calcyphosine like [Source:HGNC Symbol;Acc:HGNC:28375])

HSP 1 Score: 225.713 bits (574), Expect = 1.380e-73
Identity = 109/208 (52.40%), Postives = 156/208 (75.00%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MA + + D E+ ++A+K+L+TA DP+ +LRLQCL  G++GIKGL R FRIM+DDN+R L   EF  G+  + +++   E+E+LF+  D+DG+ TI   EFL  LRPP+S  R ++I +AF  +DKTGDGVITIEDL+ VYNA  HPK QNGEWSE +VF++FL +F++P D DG+VT +EF++YY+ +S SID + YF +MMR ++K+
Sbjct:    1 MAGTARHDREMAIQAKKKLTTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDDNNRTLDFKEFMKGLNDYAVVMEKEEVEELFRRFDKDGNGTIDFNEFLLTLRPPMSRARKEVIMQAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWSEEQVFRKFLDNFDSPYDKDGLVTPEEFMNYYAGVSASIDTDVYFIIMMRTAWKL 208          
BLAST of Calcyphosin-like protein vs. Ensembl Human
Match: CAPSL (calcyphosine like [Source:HGNC Symbol;Acc:HGNC:28375])

HSP 1 Score: 207.608 bits (527), Expect = 8.490e-67
Identity = 100/189 (52.91%), Postives = 142/189 (75.13%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSIS 643
            MA + + D E+ ++A+K+L+TA DP+ +LRLQCL  G++GIKGL R FRIM+DDN+R L   EF  G+  + +++   E+E+LF+  D+DG+ TI   EFL  LRPP+S  R ++I +AF  +DKTGDGVITIEDL+ VYNA  HPK QNGEWSE +VF++FL +F++P D DG+VT +EF++YY+ +S
Sbjct:    1 MAGTARHDREMAIQAKKKLTTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDDNNRTLDFKEFMKGLNDYAVVMEKEEVEELFRRFDKDGNGTIDFNEFLLTLRPPMSRARKEVIMQAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWSEEQVFRKFLDNFDSPYDKDGLVTPEEFMNYYAGVS 189          
BLAST of Calcyphosin-like protein vs. Ensembl Human
Match: CAPSL (calcyphosine like [Source:HGNC Symbol;Acc:HGNC:28375])

HSP 1 Score: 191.815 bits (486), Expect = 1.028e-60
Identity = 94/177 (53.11%), Postives = 132/177 (74.58%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVT 607
            MA + + D E+ ++A+K+L+TA DP+ +LRLQCL  G++GIKGL R FRIM+DDN+R L   EF  G+  + +++   E+E+LF+  D+DG+ TI   EFL  LRPP+S  R ++I +AF  +DKTGDGVITIEDL+ VYNA  HPK QNGEWSE +VF++FL +F++P D DG+V+
Sbjct:    1 MAGTARHDREMAIQAKKKLTTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDDNNRTLDFKEFMKGLNDYAVVMEKEEVEELFRRFDKDGNGTIDFNEFLLTLRPPMSRARKEVIMQAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWSEEQVFRKFLDNFDSPYDKDGLVS 177          
BLAST of Calcyphosin-like protein vs. Ensembl Celegans
Match: cal-8 (CALmodulin related genes [Source:UniProtKB/TrEMBL;Acc:Q09980])

HSP 1 Score: 55.8398 bits (133), Expect = 1.426e-9
Identity = 41/141 (29.08%), Postives = 71/141 (50.35%), Query Frame = 2
Query:  218 FRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNR-LKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            FR  + + D  +++ E +  +   G   ++S+IE + +  D DG+  I + EFL  LR  + D +  + ++  F + DK GDGVI+I+DL  V        CQ GE       +E +K  +   D DG++ + EF++    
Sbjct:   13 FREFDKNGDGRITRQELEVALLQLGEKASNSKIETMIEQADLDGNGCIDIDEFLNVLRRQICDPKEERELRDVFNVFDKNGDGVISIDDLIFVM-------CQLGEKLTETEAKEMIK--QGDLDHDGMIDFQEFVNIIKG 144          
BLAST of Calcyphosin-like protein vs. Ensembl Celegans
Match: cmd-1 (Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O16305])

HSP 1 Score: 55.0694 bits (131), Expect = 2.855e-9
Identity = 40/148 (27.03%), Postives = 71/148 (47.97%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            I      F + + D D  ++  E    ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+   ++
Sbjct:   10 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVM-------TNLGEKLTDEEVDEMIR--EADIDGDGQVNYEEFVTMMTT 148          
BLAST of Calcyphosin-like protein vs. Ensembl Celegans
Match: cal-2 (CALmodulin related genes; Calmodulin-like protein [Source:UniProtKB/TrEMBL;Acc:G5EEG0])

HSP 1 Score: 52.373 bits (124), Expect = 3.320e-8
Identity = 41/158 (25.95%), Postives = 76/158 (48.10%), Query Frame = 2
Query:  188 ASGIKGLSRR--------FRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
             SG  GL+          FR+ + D +  +S  E    ++  G   T+ E+ D+   +D DGS TI   EF + ++    +N  ++I++AF + D+ G+G IT ++ ++      H   Q   +S+  V  E +   +   D DG + ++EF   +S+
Sbjct:   23 VSGFDGLNEEEIMEYKAAFRLFDKDGNGSISSKELGVAMRSLGQNPTEQELLDMVNEVDIDGSGTIDFGEFCQMMKRMNKENDSEMIREAFRVFDRDGNGFITADEFRYF---MTHMGDQ---FSDQEV-DEIIAEIDI--DGDGQIDYEEFASTFSA 171          
BLAST of Calcyphosin-like protein vs. Ensembl Celegans
Match: cmd-1 (Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O16305])

HSP 1 Score: 50.447 bits (119), Expect = 5.614e-8
Identity = 35/116 (30.17%), Postives = 60/116 (51.72%), Query Frame = 2
Query:  278 IKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFL 622
            ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+
Sbjct:    1 MRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVM-------TNLGEKLTDEEVDEMIR--EADIDGDGQVNYEEFV 107          
BLAST of Calcyphosin-like protein vs. Ensembl Celegans
Match: cal-4 (CALmodulin related genes [Source:UniProtKB/TrEMBL;Acc:G5EDZ8])

HSP 1 Score: 51.2174 bits (121), Expect = 9.058e-8
Identity = 41/163 (25.15%), Postives = 77/163 (47.24%), Query Frame = 2
Query:  170 QCLCNGA-----SGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGE-WSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSI 640
            Q +C+ A       ++  ++ F++ + D +  ++  E    ++  GL  T+ E+ ++    D DG+  I   EF + ++    +   +LI+ AF + DK G+G IT ++ KH            GE +SE  V  E ++  E   D D  +  DEF++  + I
Sbjct:    8 QSVCSDADEFTPEELQEFAQAFKLFDKDGNNTMNIKELGEAMRMLGLNPTEEELLNMVNEYDVDGNGKIDFGEFCKMMKEMNKETDQELIRLAFKVFDKDGNGYITAQEFKHFMTTM-------GERFSEEEV-DEIIR--EVDKDGDEQIDLDEFVNMVAPI 160          
BLAST of Calcyphosin-like protein vs. Ensembl Fly
Match: CG31345 (gene:FBgn0051345 transcript:FBtr0082689)

HSP 1 Score: 224.172 bits (570), Expect = 3.205e-73
Identity = 111/205 (54.15%), Postives = 150/205 (73.17%), Query Frame = 2
Query:   98 DMELRMKARKELSTA--KDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFE--APNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            + EL+  A++EL+    KDP+ KLRLQC   GA+GI GLSR FR+M+DD  + LS  EFK G+   GL LTDSEI+++F   D DGS  I MTEFL  LRPP++++R+ +I+KAF  MD  GDG IT+ DLK+VY+   HPK  +GE +E ++F +FLK+FE  APN  DGIVT +EF++YY++IS SIDN+ YFDLMMR ++K+
Sbjct:   10 EAELKNLAKRELANGLDKDPIYKLRLQCFSRGATGILGLSRSFRVMDDDGSKSLSPEEFKKGVTDIGLDLTDSEIDEMFSRFDTDGSGNINMTEFLVKLRPPMNNSRISIIEKAFDKMDANGDGQITVTDLKNVYSVRDHPKYLSGEMTENQIFTQFLKNFEVGAPN-PDGIVTREEFINYYATISASIDNDAYFDLMMRQAYKL 213          
BLAST of Calcyphosin-like protein vs. Ensembl Fly
Match: CG10126 (gene:FBgn0038088 transcript:FBtr0082686)

HSP 1 Score: 197.208 bits (500), Expect = 1.178e-62
Identity = 99/198 (50.00%), Postives = 139/198 (70.20%), Query Frame = 2
Query:  116 KARKELSTA--KDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAP-NDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            +A +EL+    KDP+TKLRL CL  GA+GI GL R FR M+DD  + L++ EF  GI+  GL +++ EI+ +F   D DGS +I MTEFL  LRPP+  +RL +I +AF  MD+  DGVITI+DLK+VY+  +HPK Q+GE SE  +  +FL +FE    + DG +T +EF++YY++IS SIDN+ +FDLMMR ++ M
Sbjct:   18 QALRELTDGEDKDPITKLRLLCLSRGATGILGLGRAFRAMDDDGSKALNEEEFITGIRDTGLDVSEEEIKQMFATFDEDGSGSINMTEFLLKLRPPMPQSRLNIIDQAFNKMDRDEDGVITIQDLKNVYSVKEHPKYQSGEKSEDEILTDFLHNFEGGRGNLDGKITREEFVNYYATISASIDNDMFFDLMMRRAYVM 215          
BLAST of Calcyphosin-like protein vs. Ensembl Fly
Match: CG10126 (gene:FBgn0038088 transcript:FBtr0082687)

HSP 1 Score: 196.438 bits (498), Expect = 3.665e-62
Identity = 99/198 (50.00%), Postives = 139/198 (70.20%), Query Frame = 2
Query:  116 KARKELSTA--KDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAP-NDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            +A +EL+    KDP+TKLRL CL  GA+GI GL R FR M+DD  + L++ EF  GI+  GL +++ EI+ +F   D DGS +I MTEFL  LRPP+  +RL +I +AF  MD+  DGVITI+DLK+VY+  +HPK Q+GE SE  +  +FL +FE    + DG +T +EF++YY++IS SIDN+ +FDLMMR ++ M
Sbjct:   30 QALRELTDGEDKDPITKLRLLCLSRGATGILGLGRAFRAMDDDGSKALNEEEFITGIRDTGLDVSEEEIKQMFATFDEDGSGSINMTEFLLKLRPPMPQSRLNIIDQAFNKMDRDEDGVITIQDLKNVYSVKEHPKYQSGEKSEDEILTDFLHNFEGGRGNLDGKITREEFVNYYATISASIDNDMFFDLMMRRAYVM 227          
BLAST of Calcyphosin-like protein vs. Ensembl Fly
Match: Cam (gene:FBgn0000253 transcript:FBtr0304964)

HSP 1 Score: 56.225 bits (134), Expect = 1.587e-9
Identity = 41/148 (27.70%), Postives = 71/148 (47.97%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            I      F + + D D  ++  E    ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+   +S
Sbjct:   10 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVM-------TNLGEKLTDEEVDEMIR--EADIDGDGQVNYEEFVTMMTS 148          
BLAST of Calcyphosin-like protein vs. Ensembl Fly
Match: Cam (gene:FBgn0000253 transcript:FBtr0088001)

HSP 1 Score: 56.225 bits (134), Expect = 1.587e-9
Identity = 41/148 (27.70%), Postives = 71/148 (47.97%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            I      F + + D D  ++  E    ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+   +S
Sbjct:   10 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVM-------TNLGEKLTDEEVDEMIR--EADIDGDGQVNYEEFVTMMTS 148          
BLAST of Calcyphosin-like protein vs. Ensembl Zebrafish
Match: capslb (calcyphosine-like b [Source:ZFIN;Acc:ZDB-GENE-050417-298])

HSP 1 Score: 223.787 bits (569), Expect = 1.647e-72
Identity = 106/211 (50.24%), Postives = 152/211 (72.04%), Query Frame = 2
Query:   68 KLTMAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            +  MA + + + E+ + A+K+L+   DP+ +LRLQCL  G +GIKGL R FRIM+DDN+R L   EF  G+  +G+L+   E  +LF+  DRDGS  I   EFL  LRPP+S+ R +++ +AF  +DKTGDGVIT+EDLK VY+  KHPK  NGEW+E ++F++FL SF++P+D DG VT +EF++YYS +S SID + YF LMMRN++++
Sbjct:   37 RWRMAGTSRHNREMMINAKKQLAECSDPIERLRLQCLSRGCAGIKGLGRTFRIMDDDNNRTLDLKEFIKGLNDYGVLMEKEEAINLFQQFDRDGSGQIDFDEFLITLRPPMSNARKEVVLQAFKKLDKTGDGVITVEDLKGVYDVRKHPKYLNGEWTEDQIFRKFLDSFDSPDDKDGKVTKEEFMNYYSGVSASIDTDIYFILMMRNAWRL 247          
BLAST of Calcyphosin-like protein vs. Ensembl Zebrafish
Match: capsla (calcyphosine-like a [Source:ZFIN;Acc:ZDB-GENE-030131-7291])

HSP 1 Score: 205.682 bits (522), Expect = 3.148e-66
Identity = 91/187 (48.66%), Postives = 135/187 (72.19%), Query Frame = 2
Query:  140 AKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            A D L  LR QCL  GA+GIKGL R FR M+DD  + L   EF  G++ +G+ +   + + +F +MD+DGS +I   EFL  LRPP+S  R+++I++AF   DK+GDGV+T+EDL+ VYN+  HPK ++GEW+ET+VF  FL SF++P+D DG VT +EF++YYS +S S+D++ YF  MM++++K+
Sbjct:    2 AADALQDLRQQCLSRGAAGIKGLGRMFRSMDDDGSKSLDFQEFVRGLQDYGVSVGRDQAQQIFAMMDKDGSGSINFDEFLEKLRPPMSSARMQVIRQAFQKFDKSGDGVVTVEDLQGVYNSKHHPKYKSGEWTETQVFHSFLDSFDSPHDKDGKVTLEEFVNYYSGVSASVDSDEYFISMMKSAWKL 188          
BLAST of Calcyphosin-like protein vs. Ensembl Zebrafish
Match: calm2a (calmodulin 2a (phosphorylase kinase, delta) [Source:ZFIN;Acc:ZDB-GENE-030804-3])

HSP 1 Score: 55.8398 bits (133), Expect = 2.749e-9
Identity = 40/148 (27.03%), Postives = 71/148 (47.97%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            I      F + + D D  ++  E    ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+   ++
Sbjct:   10 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVM-------TNLGEKLTDEEVDEMIR--EADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calcyphosin-like protein vs. Ensembl Zebrafish
Match: calm3a (calmodulin 3a (phosphorylase kinase, delta) [Source:ZFIN;Acc:ZDB-GENE-030131-5590])

HSP 1 Score: 55.8398 bits (133), Expect = 2.859e-9
Identity = 40/148 (27.03%), Postives = 71/148 (47.97%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            I      F + + D D  ++  E    ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+   ++
Sbjct:   10 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVM-------TNLGEKLTDEEVDEMIR--EADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calcyphosin-like protein vs. Ensembl Zebrafish
Match: calm3b (calmodulin 3b (phosphorylase kinase, delta) [Source:ZFIN;Acc:ZDB-GENE-030131-527])

HSP 1 Score: 55.8398 bits (133), Expect = 2.859e-9
Identity = 40/148 (27.03%), Postives = 71/148 (47.97%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            I      F + + D D  ++  E    ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+   ++
Sbjct:   10 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVM-------TNLGEKLTDEEVDEMIR--EADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calcyphosin-like protein vs. Ensembl Xenopus
Match: capsl (calcyphosine like [Source:NCBI gene;Acc:549278])

HSP 1 Score: 230.72 bits (587), Expect = 1.170e-75
Identity = 108/208 (51.92%), Postives = 153/208 (73.56%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            M  + + D ++ ++A+K LS+  DP+ KLRLQCL  G++GIKGL R FRIM+DD ++ L   EF  G+  +G+++  +E ++LF + D+DGS TI   EFL  LRPP+S+ R  +I +AF  +DK+GDGV+TIEDL+ VYNA  HPK QNGEW+E +VF+ FL +F++P D DG VT DEFL+YYS +S SID++ YF LMMRN++K+
Sbjct:    1 MPGTARHDRDMALQAKKNLSSCTDPVEKLRLQCLSRGSAGIKGLGRVFRIMDDDGNKTLDFKEFSKGLSDYGVMMDKTETQELFSVFDKDGSGTIDFDEFLVTLRPPMSNARKDMILQAFRKLDKSGDGVVTIEDLRGVYNAKHHPKYQNGEWTEDQVFRSFLDNFDSPYDKDGQVTKDEFLNYYSGVSASIDSDTYFILMMRNAWKL 208          
BLAST of Calcyphosin-like protein vs. Ensembl Xenopus
Match: capsl (calcyphosine like [Source:NCBI gene;Acc:549278])

HSP 1 Score: 225.713 bits (574), Expect = 1.051e-73
Identity = 105/208 (50.48%), Postives = 151/208 (72.60%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            M  + + D ++ ++A+K LS+  DP+ KLRLQCL  G++GIKGL R FRIM+DD ++ L   EF  G+  +G+++  +E ++LF + D+DGS TI   EFL  LRPP+S+ R  +I +AF  +DK+GDGV+TIEDL+ VYNA  HPK QNGEW+E +VF+ FL +F++P D DG VT DEF++YY+ +S SID + YF  MMRN++K+
Sbjct:    1 MPGTARHDRDMALQAKKNLSSCTDPVEKLRLQCLSRGSAGIKGLGRVFRIMDDDGNKTLDFKEFSKGLSDYGVMMDKTETQELFSVFDKDGSGTIDFDEFLVTLRPPMSNARKDMILQAFRKLDKSGDGVVTIEDLRGVYNAKHHPKYQNGEWTEDQVFRSFLDNFDSPYDKDGQVTPDEFMNYYAGVSASIDTDIYFITMMRNAWKL 208          
BLAST of Calcyphosin-like protein vs. Ensembl Xenopus
Match: capsl (calcyphosine like [Source:NCBI gene;Acc:549278])

HSP 1 Score: 223.402 bits (568), Expect = 7.221e-73
Identity = 103/198 (52.02%), Postives = 146/198 (73.74%), Query Frame = 2
Query:  107 LRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            + ++A+K LS+  DP+ KLRLQCL  G++GIKGL R FRIM+DD ++ L   EF  G+  +G+++  +E ++LF + D+DGS TI   EFL  LRPP+S+ R  +I +AF  +DK+GDGV+TIEDL+ VYNA  HPK QNGEW+E +VF+ FL +F++P D DG VT DEF++YY+ +S SID + YF  MMRN++K+
Sbjct:    1 MALQAKKNLSSCTDPVEKLRLQCLSRGSAGIKGLGRVFRIMDDDGNKTLDFKEFSKGLSDYGVMMDKTETQELFSVFDKDGSGTIDFDEFLVTLRPPMSNARKDMILQAFRKLDKSGDGVVTIEDLRGVYNAKHHPKYQNGEWTEDQVFRSFLDNFDSPYDKDGQVTPDEFMNYYAGVSASIDTDIYFITMMRNAWKL 198          
BLAST of Calcyphosin-like protein vs. Ensembl Xenopus
Match: caps (calcyphosine [Source:NCBI gene;Acc:100037881])

HSP 1 Score: 217.624 bits (553), Expect = 1.496e-70
Identity = 106/208 (50.96%), Postives = 146/208 (70.19%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MA   + + E+  KA+++    +DP+ KLRLQCL  GASGIKGL R FRIM+D+    L   EF  G++ FG+ L  SE+ D+F   D +G+ TI   EFL ++RPP+S+ R ++I  AF  MD+TGDGVITIEDLK VYN   H K +NGEW+E +VFQ FL +F++PN+ DG VT +EFL+YYS +S SID++ YF +MM+N +K+
Sbjct:    1 MASMTQLEREMMAKAQRQYPQCQDPVEKLRLQCLARGASGIKGLGRAFRIMDDNRSSTLDLEEFSKGLQNFGISLQPSEVLDIFHQFDTNGNGTINFDEFLISIRPPMSNARRQVILDAFKKMDRTGDGVITIEDLKGVYNPKFHQKYRNGEWNERQVFQNFLDNFDSPNNKDGQVTEEEFLNYYSGVSASIDSDAYFVVMMKNEWKI 208          
BLAST of Calcyphosin-like protein vs. Ensembl Xenopus
Match: ENSXETT00000016513.1 (calcyphosine 2 [Source:NCBI gene;Acc:100380015])

HSP 1 Score: 90.8929 bits (224), Expect = 1.076e-21
Identity = 50/170 (29.41%), Postives = 91/170 (53.53%), Query Frame = 2
Query:  185 GASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSF 694
            G   + GL + FR ++++ D  L + +FK  +K F L +++   E  +K++D      +   +F RAL   +++ R   ++KAF  +D    G+I + D++  Y A KHP+  +G  SE  +   FLK+ +A       V++ EF D+Y  +S  I ++  F  ++RNS+
Sbjct:   13 GVQTLTGLGKFFRQVDENGDGILQKAKFKQALKVFHLDVSEEIFESFWKILDEKHEGKLDYGKFTRALIGEMNEYRKTFVQKAFMKLDPNKSGIIPMIDIRKFYCARKHPQVLSGHSSEEEILSSFLKTLQAACRNPKEVSYCEFEDFYEGLSIEILSDNDFINILRNSW 182          
BLAST of Calcyphosin-like protein vs. Ensembl Mouse
Match: Capsl (calcyphosine-like [Source:MGI Symbol;Acc:MGI:1922818])

HSP 1 Score: 233.032 bits (593), Expect = 1.260e-76
Identity = 109/208 (52.40%), Postives = 154/208 (74.04%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MA + + D E+ ++++K+LSTA DP+ +LRLQCL  G++GIKGL R FRIM+D+N+R L   EF  G+  + +++   E E+LF+  DRDGS TI   EFL  LRPP+S  R ++I KAF  +DKTGDGVITIEDL+ VYNA  HPK QNGEW+E +VF++FL +F++P D DG+VT +EF++YY+ +S SID + YF +MM  ++K+
Sbjct:    1 MAGTARHDREMAIQSKKKLSTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDNNNRTLDFKEFLKGLNDYAVVMEKEEAEELFQRFDRDGSGTIDFNEFLLTLRPPMSRARKEVIMKAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWTEEQVFRKFLDNFDSPYDKDGLVTPEEFMNYYAGVSASIDTDVYFIIMMTTAWKL 208          
BLAST of Calcyphosin-like protein vs. Ensembl Mouse
Match: Capsl (calcyphosine-like [Source:MGI Symbol;Acc:MGI:1922818])

HSP 1 Score: 104.76 bits (260), Expect = 1.431e-27
Identity = 51/105 (48.57%), Postives = 73/105 (69.52%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALR 391
            MA + + D E+ ++++K+LSTA DP+ +LRLQCL  G++GIKGL R FRIM+D+N+R L   EF  G+  + +++   E E+LF+  DRDGS TI   EFL  LR
Sbjct:    1 MAGTARHDREMAIQSKKKLSTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDNNNRTLDFKEFLKGLNDYAVVMEKEEAEELFQRFDRDGSGTIDFNEFLLTLR 105          
BLAST of Calcyphosin-like protein vs. Ensembl Mouse
Match: Caps2 (calcyphosphine 2 [Source:MGI Symbol;Acc:MGI:2441980])

HSP 1 Score: 80.1073 bits (196), Expect = 1.861e-16
Identity = 46/183 (25.14%), Postives = 95/183 (51.91%), Query Frame = 2
Query:  158 KLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGS--NTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            KL+ Q    GA  + GL R F+ ++ + +  L + +F+  +K F L +++ + E  + ++   G   N +   EF RA+   +++ R   ++KAF  +D    G++++ D++  Y A KHP+  +G  +E  +   FL++ +        V++ EF DYY  +S  +  +  F  ++R  +++
Sbjct:  410 KLKEQLHKKGARILTGLGRYFQGLDKEGNGLLEKADFQQALKTFHLEVSEQDFESFWLILQGYGHSKNKVDYGEFKRAIFGEMNEYRKSFVRKAFMQLDFNKTGIVSVIDIRKCYCAKKHPRVISGHSTEEEIKSSFLETLKGTCSKCDEVSYGEFEDYYEGLSIGVAGDEDFVNILRIPWRI 592          
BLAST of Calcyphosin-like protein vs. Ensembl Mouse
Match: Caps2 (calcyphosphine 2 [Source:MGI Symbol;Acc:MGI:2441980])

HSP 1 Score: 80.1073 bits (196), Expect = 1.909e-16
Identity = 46/183 (25.14%), Postives = 95/183 (51.91%), Query Frame = 2
Query:  158 KLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGS--NTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            KL+ Q    GA  + GL R F+ ++ + +  L + +F+  +K F L +++ + E  + ++   G   N +   EF RA+   +++ R   ++KAF  +D    G++++ D++  Y A KHP+  +G  +E  +   FL++ +        V++ EF DYY  +S  +  +  F  ++R  +++
Sbjct:  368 KLKEQLHKKGARILTGLGRYFQGLDKEGNGLLEKADFQQALKTFHLEVSEQDFESFWLILQGYGHSKNKVDYGEFKRAIFGEMNEYRKSFVRKAFMQLDFNKTGIVSVIDIRKCYCAKKHPRVISGHSTEEEIKSSFLETLKGTCSKCDEVSYGEFEDYYEGLSIGVAGDEDFVNILRIPWRI 550          
BLAST of Calcyphosin-like protein vs. Ensembl Mouse
Match: Calm3 (calmodulin 3 [Source:MGI Symbol;Acc:MGI:103249])

HSP 1 Score: 55.8398 bits (133), Expect = 3.085e-9
Identity = 40/148 (27.03%), Postives = 71/148 (47.97%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            I      F + + D D  ++  E    ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+   ++
Sbjct:   10 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVM-------TNLGEKLTDEEVDEMIR--EADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calcyphosin-like protein vs. UniProt/SwissProt
Match: sp|Q6P8Y1|CAPSL_MOUSE (Calcyphosin-like protein OS=Mus musculus OX=10090 GN=Capsl PE=2 SV=4)

HSP 1 Score: 233.032 bits (593), Expect = 8.813e-76
Identity = 109/208 (52.40%), Postives = 154/208 (74.04%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MA + + D E+ ++++K+LSTA DP+ +LRLQCL  G++GIKGL R FRIM+D+N+R L   EF  G+  + +++   E E+LF+  DRDGS TI   EFL  LRPP+S  R ++I KAF  +DKTGDGVITIEDL+ VYNA  HPK QNGEW+E +VF++FL +F++P D DG+VT +EF++YY+ +S SID + YF +MM  ++K+
Sbjct:    1 MAGTARHDREMAIQSKKKLSTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDNNNRTLDFKEFLKGLNDYAVVMEKEEAEELFQRFDRDGSGTIDFNEFLLTLRPPMSRARKEVIMKAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWTEEQVFRKFLDNFDSPYDKDGLVTPEEFMNYYAGVSASIDTDVYFIIMMTTAWKL 208          
BLAST of Calcyphosin-like protein vs. UniProt/SwissProt
Match: sp|Q8WWF8|CAPSL_HUMAN (Calcyphosin-like protein OS=Homo sapiens OX=9606 GN=CAPSL PE=2 SV=4)

HSP 1 Score: 225.713 bits (574), Expect = 6.629e-73
Identity = 109/208 (52.40%), Postives = 156/208 (75.00%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MA + + D E+ ++A+K+L+TA DP+ +LRLQCL  G++GIKGL R FRIM+DDN+R L   EF  G+  + +++   E+E+LF+  D+DG+ TI   EFL  LRPP+S  R ++I +AF  +DKTGDGVITIEDL+ VYNA  HPK QNGEWSE +VF++FL +F++P D DG+VT +EF++YY+ +S SID + YF +MMR ++K+
Sbjct:    1 MAGTARHDREMAIQAKKKLTTATDPIERLRLQCLARGSAGIKGLGRVFRIMDDDNNRTLDFKEFMKGLNDYAVVMEKEEVEELFRRFDKDGNGTIDFNEFLLTLRPPMSRARKEVIMQAFRKLDKTGDGVITIEDLREVYNAKHHPKYQNGEWSEEQVFRKFLDNFDSPYDKDGLVTPEEFMNYYAGVSASIDTDVYFIIMMRTAWKL 208          
BLAST of Calcyphosin-like protein vs. UniProt/SwissProt
Match: sp|Q0VCC0|CAYP1_BOVIN (Calcyphosin OS=Bos taurus OX=9913 GN=CAPS PE=2 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 2.422e-53
Identity = 81/183 (44.26%), Postives = 125/183 (68.31%), Query Frame = 2
Query:  152 LTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            + KLR QCL  GASGI+G++R FR ++ D  R L   E + G+   GL+L  +E+E + +  DRDGS T+ + EFLRALRPP+S  R  ++  AF  +D++GDGV+T++DL+ VY+   HPK ++GEW+E +V + FL +F++ ++ DG VT  EF DYYS +S S+D +  F  MM +++++
Sbjct:    8 MEKLRAQCLSRGASGIQGVARFFRRLDQDGSRSLDVRELQRGLAELGLVLDTAEMEGVCRRWDRDGSGTLDLEEFLRALRPPMSQAREAVVTAAFAKLDRSGDGVVTVDDLRGVYSGRTHPKVRSGEWTEEQVLRHFLDNFDS-SEKDGQVTLAEFQDYYSGVSASMDTDEEFVAMMTSAWRL 189          
BLAST of Calcyphosin-like protein vs. UniProt/SwissProt
Match: sp|P41150|CAYP1_RABIT (Calcyphosin OS=Oryctolagus cuniculus OX=9986 GN=CAPS PE=1 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 3.293e-52
Identity = 82/190 (43.16%), Postives = 124/190 (65.26%), Query Frame = 2
Query:  131 LSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            + TA   + KLR QCL  GA+GI+GL+R FR ++ D  R L   E + G+   GL L ++E + + +  DR+GS T+ + EFLRALRPP+S  R  +I  AF  +D++GDGV+T+ DL+ VY+   HPK + GEW+E  V + FL +F++ ++ DG VT  EF DYYS +S S+D +  F  MM +++++
Sbjct:    1 MDTADAIVEKLRAQCLARGAAGIQGLARFFRRLDRDGSRSLDAGELRQGLAKLGLALDEAEAQSVCRRWDRNGSGTLDLEEFLRALRPPMSQAREAVIAAAFAKLDRSGDGVVTLGDLRGVYSGRNHPKVRTGEWTEDEVLRRFLDNFDS-SEKDGQVTLAEFQDYYSGLSASVDTDEEFVAMMSSAWQL 189          
BLAST of Calcyphosin-like protein vs. UniProt/SwissProt
Match: sp|Q13938|CAYP1_HUMAN (Calcyphosin OS=Homo sapiens OX=9606 GN=CAPS PE=1 SV=2)

HSP 1 Score: 174.481 bits (441), Expect = 4.448e-52
Identity = 83/195 (42.56%), Postives = 127/195 (65.13%), Query Frame = 2
Query:  116 KARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            + R  +      + KLR QCL  GASGI+GL+R FR ++ D  R L   EF+ G+   GL+L  +E E + +  DR+GS T+ + EFLRALRPP+S  R  +I  AF  +D++GDGV+T++DL+ VY+   HPK ++GEW+E  V + FL +F++ ++ DG VT  EF DYYS +S S++ +  F  MM +++++
Sbjct:   82 EPRPSMDAVDATMEKLRAQCLSRGASGIQGLARFFRQLDRDGSRSLDADEFRQGLAKLGLVLDQAEAEGVCRKWDRNGSGTLDLEEFLRALRPPMSQAREAVIAAAFAKLDRSGDGVVTVDDLRGVYSGRAHPKVRSGEWTEDEVLRRFLDNFDS-SEKDGQVTLAEFQDYYSGVSASMNTDEEFVAMMTSAWQL 275          
BLAST of Calcyphosin-like protein vs. TrEMBL
Match: A0A1I8HIY9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017698g1 PE=4 SV=1)

HSP 1 Score: 280.411 bits (716), Expect = 4.974e-92
Identity = 130/208 (62.50%), Postives = 166/208 (79.81%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            M+FS KD+ EL+MKARKEL+  +DP+ +LRL C+  GA+GIKG SR+FRIM+DD ++ LS  EF+ G+  FGL LTD E++  F  +D D S +I  +EFL  LRPPL+++RLKLIK AF  MDKTGDG IT++DLK +YNA +HPK QNGEWSE +VF EFLK+FEAPN  DG+VTW+EFL+YYS ISNSID++ YFDLMMRNS+K+
Sbjct:    1 MSFSQKDNYELQMKARKELTQEQDPVKRLRLSCMSRGAAGIKGFSRQFRIMDDDGNKRLSMAEFRKGVADFGLDLTDEEVKAAFNQVDTDQSGSIDFSEFLAVLRPPLNESRLKLIKAAFQKMDKTGDGQITVKDLKGIYNARQHPKYQNGEWSEDQVFTEFLKTFEAPNTTDGVVTWEEFLNYYSGISNSIDHDSYFDLMMRNSWKL 208          
BLAST of Calcyphosin-like protein vs. TrEMBL
Match: A0A267H6P0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002637g1 PE=4 SV=1)

HSP 1 Score: 244.588 bits (623), Expect = 6.836e-78
Identity = 116/210 (55.24%), Postives = 155/210 (73.81%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAK--DPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MA +D+   EL  KA+K+L++ K  DPL KLRL CL  GASGIKG+ R+FRI++DD  R L + EF  G   FG  L+  E++ +F  +D+D S T+   EFL+ALRPP+S  R +LI KAF  +D+TGDGVIT+EDL+ VYN   HPK QNG+W+E  VF+EFL++FE PNDADG+VT +EF +YYS +S S+DN+ YFDLMMRN++K+
Sbjct:    1 MAATDRGSAELAAKAKKQLASGKAADPLEKLRLVCLSRGASGIKGIGRQFRIIDDDGSRSLDRREFYKGCTDFGADLSKEEMDQIFDRIDKDRSGTLDFDEFLQALRPPMSSARRELITKAFRKLDRTGDGVITVEDLRGVYNCKHHPKYQNGQWTEDDVFKEFLRTFETPNDADGVVTKEEFFNYYSGVSASVDNDAYFDLMMRNAYKI 210          
BLAST of Calcyphosin-like protein vs. TrEMBL
Match: A0A437CVX0 (Uncharacterized protein OS=Oryzias javanicus OX=123683 GN=OJAV_G00124660 PE=4 SV=1)

HSP 1 Score: 240.736 bits (613), Expect = 2.286e-76
Identity = 117/208 (56.25%), Postives = 152/208 (73.08%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MA + + D E+ +KA+++LS   DP+ KLRLQCL  G+SGIKGL R FR+M+D+ +R L   EF  G+  +GLLL   E   LF+  DRDGS TI   EFL ALRPP+S  RL+++ +AF  MDKTGDGVIT+EDL+ +YNA  HPK QNGE SE +VF+ FL SF++P D DG VT +EFLDYYS +S SID++ YF LMMRN++K+
Sbjct:    1 MAGTSRHDHEMAVKAKRQLSECSDPVEKLRLQCLIRGSSGIKGLGRTFRVMDDNQNRSLDLKEFLKGLNDYGLLLEKEEAASLFQHFDRDGSGTISFDEFLVALRPPMSKARLEVVMQAFRKMDKTGDGVITVEDLRGIYNAKYHPKYQNGELSEDQVFRTFLDSFDSPYDKDGKVTKEEFLDYYSGVSASIDSDVYFILMMRNAWKL 208          
BLAST of Calcyphosin-like protein vs. TrEMBL
Match: A0A1I8I1U9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 242.662 bits (618), Expect = 6.164e-76
Identity = 114/211 (54.03%), Postives = 154/211 (72.99%), Query Frame = 2
Query:   74 TMAFSDKDDMELRMKARKELSTAK--DPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
             M  +D+   EL  KA+K+L++ K  DPL KLRL CL  GASGI+G+ R+FRI++DD  R L + EF  G   FG  L+  E++ +F  +D+D S T+   EFL+ALRPP+S  R +LI KAF  +D+TGDGVIT+EDL+ VYN   HPK QNG+W+E  VF+EFL++FE PNDADG+VT +EF +YYS +S S+DN+ YFDLMMRN++K+
Sbjct:   72 AMVATDRGSAELAAKAKKQLASGKAADPLEKLRLVCLSRGASGIEGIGRQFRIIDDDGSRSLDRREFYKGCTDFGADLSKEEMDQIFDRIDKDRSGTLDFDEFLQALRPPMSSARRELITKAFRKLDRTGDGVITVEDLRGVYNCKHHPKYQNGQWTEDDVFKEFLRTFETPNDADGVVTKEEFFNYYSGVSASVDNDAYFDLMMRNAYKI 282          
BLAST of Calcyphosin-like protein vs. TrEMBL
Match: A0A267F2D9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002765g1 PE=4 SV=1)

HSP 1 Score: 239.195 bits (609), Expect = 8.839e-76
Identity = 117/209 (55.98%), Postives = 152/209 (72.73%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTA-KDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MA +D+   EL  KA+K+L++  KDPL KLRL CL  GASGIKGL R+FRI++DD  + L + EF  G   FG  L+  E+E +F  +D+DGS ++   EFL ALRPP+S+ R +LI KAF  +DKT DGVIT+EDLK VYN   HPK +NGEW+E  VF+EFL++FE P DADG VT +EF +YYS +S SIDN+ YFDLMMR ++K+
Sbjct:    1 MAATDRGSAELAAKAKKQLASGTKDPLEKLRLTCLSRGASGIKGLGRQFRIIDDDGSKSLDRREFFKGCTDFGADLSKEEMEAIFTRIDKDGSGSLDFDEFLVALRPPMSNARRELITKAFQKLDKTRDGVITVEDLKGVYNCKHHPKFRNGEWTEEDVFKEFLRTFETPGDADGTVTKEEFFNYYSGVSASIDNDAYFDLMMRTAYKI 209          
BLAST of Calcyphosin-like protein vs. Ensembl Cavefish
Match: capslb (calcyphosine like [Source:NCBI gene;Acc:103021302])

HSP 1 Score: 219.935 bits (559), Expect = 1.078e-71
Identity = 104/208 (50.00%), Postives = 150/208 (72.12%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MA + + D ++ + A+K LS A DP+ +LRLQCL  G++GIKGL R FRIM+DDN R L   EF  G+  +G+++   E  +LF+  D+DGS  I   EFL  LRPP+S+ R ++I +AF  +DKTGDGVIT+EDL+ VYN   HPK QNGEW+E ++F++FL SF++ +D DG VT +EF++YY+ +S SID + YF +MM+N++K+
Sbjct:    1 MAGTSRHDRQMAIDAKKRLSEATDPVERLRLQCLARGSAGIKGLGRVFRIMDDDNSRNLELKEFMKGLNDYGIMMEKDEAMELFRTFDKDGSGQIDFDEFLITLRPPMSNARKEVIMQAFRKLDKTGDGVITVEDLRGVYNVKNHPKYQNGEWTEDQIFRKFLDSFDS-DDKDGKVTQEEFMNYYAGVSASIDTDVYFIVMMKNAWKL 207          
BLAST of Calcyphosin-like protein vs. Ensembl Cavefish
Match: capslb (calcyphosine like [Source:NCBI gene;Acc:103021302])

HSP 1 Score: 219.164 bits (557), Expect = 2.492e-71
Identity = 105/208 (50.48%), Postives = 150/208 (72.12%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MA + + D ++ + A+K LS A DP+ +LRLQCL  G++GIKGL R FRIM+DDN R L   EF  G+  +G+++   E  +LF+  D+DGS  I   EFL  LRPP+S+ R ++I +AF  +DKTGDGVIT+EDL+ VYN   HPK QNGEW+E ++F++FL SF++ +D DG VT +EF++YYS +S SID++ YF LMM+ ++K+
Sbjct:    1 MAGTSRHDRQMAIDAKKRLSEATDPVERLRLQCLARGSAGIKGLGRVFRIMDDDNSRNLELKEFMKGLNDYGIMMEKDEAMELFRTFDKDGSGQIDFDEFLITLRPPMSNARKEVIMQAFRKLDKTGDGVITVEDLRGVYNVKNHPKYQNGEWTEDQIFRKFLDSFDS-DDKDGKVTKEEFVNYYSGVSASIDSDVYFILMMKTAWKL 207          
BLAST of Calcyphosin-like protein vs. Ensembl Cavefish
Match: capsla (calcyphosin-like protein [Source:NCBI gene;Acc:103033765])

HSP 1 Score: 201.445 bits (511), Expect = 1.254e-64
Identity = 91/185 (49.19%), Postives = 136/185 (73.51%), Query Frame = 2
Query:  146 DPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            D L +LR QCL  GA+GIKGL R FRIM+DD  + L+  EF+ G++ +G+ +   + + +F ++D+D S TI   EFL  LRPP+S  R+++IK+AF  +D TGDG++TIEDL+ VYN+  HPK ++GEW+E +VF+ FL SF++P + DG VT +EF++YYS +S SID++ YF  MM+N++K+
Sbjct:    3 DVLEELRQQCLSRGAAGIKGLGRTFRIMDDDGSKSLNLQEFQKGLETYGVNVGLEKAKQIFTIIDKDRSGTIDFNEFLEKLRPPMSSTRVQVIKQAFQKLDSTGDGLVTIEDLQGVYNSKHHPKYKSGEWTEEQVFRSFLDSFDSPYEKDGKVTLEEFVNYYSGVSASIDDDAYFVTMMQNAWKL 187          
BLAST of Calcyphosin-like protein vs. Ensembl Cavefish
Match: calm3a (calmodulin [Source:NCBI gene;Acc:103037741])

HSP 1 Score: 55.8398 bits (133), Expect = 2.423e-9
Identity = 40/148 (27.03%), Postives = 71/148 (47.97%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            I      F + + D D  ++  E    ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+   ++
Sbjct:   10 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVM-------TNLGEKLTDEEVDEMIR--EADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calcyphosin-like protein vs. Ensembl Cavefish
Match: calm2a (calmodulin [Source:NCBI gene;Acc:103037741])

HSP 1 Score: 55.8398 bits (133), Expect = 2.423e-9
Identity = 40/148 (27.03%), Postives = 71/148 (47.97%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            I      F + + D D  ++  E    ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+   ++
Sbjct:   10 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVM-------TNLGEKLTDEEVDEMIR--EADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calcyphosin-like protein vs. Ensembl Sea Lamprey
Match: CALML3 (calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:1452])

HSP 1 Score: 55.8398 bits (133), Expect = 4.725e-10
Identity = 40/148 (27.03%), Postives = 71/148 (47.97%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            I      F + + D D  ++  E    ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+   ++
Sbjct:   10 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVM-------TNLGEKLTDEEVDEMIR--EADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calcyphosin-like protein vs. Ensembl Sea Lamprey
Match: CALM3 (calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449])

HSP 1 Score: 55.8398 bits (133), Expect = 4.915e-10
Identity = 40/148 (27.03%), Postives = 71/148 (47.97%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            I      F + + D D  ++  E    ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+   ++
Sbjct:   10 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVM-------TNLGEKLTDEEVDEMIR--EADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calcyphosin-like protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006008.1 (pep scaffold:Pmarinus_7.0:GL476713:142259:146509:-1 gene:ENSPMAG00000005430.1 transcript:ENSPMAT00000006008.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.0694 bits (131), Expect = 7.424e-10
Identity = 40/148 (27.03%), Postives = 71/148 (47.97%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            I      F + + D D  ++  E    ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+   ++
Sbjct:   10 IAEFKEAFSLFDKDGDGTITVKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVM-------TNLGEKLTDEEVDEMIR--EADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calcyphosin-like protein vs. Ensembl Sea Lamprey
Match: si:rp71-17i16.4 (si:rp71-17i16.4 [Source:ZFIN;Acc:ZDB-GENE-090312-215])

HSP 1 Score: 44.2838 bits (103), Expect = 6.331e-6
Identity = 39/148 (26.35%), Postives = 66/148 (44.59%), Query Frame = 2
Query:  218 FRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFL----RALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNS 649
            F + + D    +S +E    +K  G   T  E++ + + +D DGS TI   EFL    R ++   +    + + +AF ++D  GDG I  ++LK +           GE        E +K  +   + DG + +DEFL     I+ S
Sbjct:   29 FDMFDADGGGDISTSELGKVMKLLGQTPTKEELDAIIEEVDEDGSGTIDFEEFLVMMVRQMKEESAGQTEEELAEAFRILDTNGDGYIDRDELKDIL-------LNTGENVTDLEMDELMK--DGDKNCDGRLDFDEFLKMMEGIAAS 167          
BLAST of Calcyphosin-like protein vs. Ensembl Yeast
Match: CMD1 (Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability [Source:SGD;Acc:S000000313])

HSP 1 Score: 57.7658 bits (138), Expect = 5.547e-11
Identity = 32/103 (31.07%), Postives = 58/103 (56.31%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPL-SDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNA 502
            I      F + + DN+  +S +E    ++  GL  +++E+ DL   +D DG++ I  +EFL  +   L S++  + + +AF + DK GDG+I+  +LKHV  +
Sbjct:   10 IAEFKEAFALFDKDNNGSISSSELATVMRSLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSRQLKSNDSEQELLEAFKVFDKNGDGLISAAELKHVLTS 112          
BLAST of Calcyphosin-like protein vs. Ensembl Nematostella
Match: EDO32961 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STK4])

HSP 1 Score: 228.409 bits (581), Expect = 1.969e-75
Identity = 108/208 (51.92%), Postives = 147/208 (70.67%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MA + + + EL  K +K     KDP+  LRL CL  G+SGIKGL R F+IM+DD +R L   EFK G++ +G++L   E++  F+  D DGS TI   EFL  LRPP+S  R  +I++AF  +DKTGD  IT+EDLK VYN   HPK QNGEW+E +  ++FL SF++P+D DGI+T+DEFL+YYS +S SID++ YFDLMMRN++K+
Sbjct:    1 MAATARHEKELIEKCKKNAGQTKDPVESLRLACLSRGSSGIKGLGRTFKIMDDDGNRSLDFNEFKKGLRDYGVMLEPKEVKRTFEAFDTDGSGTIDFDEFLIRLRPPMSKARKNVIQQAFNKLDKTGDNNITVEDLKGVYNVKHHPKYQNGEWTEEQCLRKFLDSFDSPDDKDGIITYDEFLNYYSGVSASIDSDAYFDLMMRNAYKL 208          
BLAST of Calcyphosin-like protein vs. Ensembl Nematostella
Match: EDO32962 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STK5])

HSP 1 Score: 160.999 bits (406), Expect = 3.162e-49
Identity = 84/171 (49.12%), Postives = 111/171 (64.91%), Query Frame = 2
Query:   95 DDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEA-PNDADGIV 604
            D+ EL++KA+K    AK P+ KL       G  GIK   R FRI +DD +R L+  EF+ G++ FG  LTD E++ LF   D+DGS ++   EFLRA+RP +S NR+++I KAF  MD TGDGVITI DLK VYNA +  + Q+GE +E  VFQ+FL +FE  P   DG V
Sbjct:    8 DERELKIKAKKMEENAKTPIEKLHAAMAGRGTCGIKMFGRAFRIFDDDGNRALNIDEFRKGMQDFGTKLTDDEVKQLFAQFDKDGSGSLDFEEFLRAVRPSMSKNRVEIIMKAFHKMDTTGDGVITIADLKKVYNARQDKRYQSGEMTEAEVFQKFLATFEPDPAKRDGKV 178          
BLAST of Calcyphosin-like protein vs. Ensembl Nematostella
Match: EDO33723 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRH0])

HSP 1 Score: 124.02 bits (310), Expect = 2.746e-35
Identity = 71/162 (43.83%), Postives = 100/162 (61.73%), Query Frame = 2
Query:  218 FRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEA-PNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            FRI +DD ++ L+  EF+ G++ +G  LTD   +  F       ++ + + + L          R ++I KAF  MD TGDGVITI DLK VYNA +  + Q+GE +E  VFQ+FL +FE  P   DG VT  EFLDYY+ +S SID++ YFDLMMR ++K+
Sbjct:    3 FRIFDDDGNKALNIDEFRKGMQDYGTKLTD---DVSFTHFSAQSTDLLLIFQGLEL--------RFRIIMKAFHKMDTTGDGVITIADLKKVYNARQDKRYQSGEMTEAEVFQKFLATFEPDPAKRDGKVTKGEFLDYYNGVSVSIDDDAYFDLMMRQAWKL 153          
BLAST of Calcyphosin-like protein vs. Ensembl Nematostella
Match: EDO37727 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SF01])

HSP 1 Score: 82.0333 bits (201), Expect = 4.020e-18
Identity = 53/207 (25.60%), Postives = 94/207 (45.41%), Query Frame = 2
Query:   68 KLTMAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRN 688
            +L  A ++K         + E    +    ++R Q     +  + G+ R FR ++ D  R   +         F  ++    +  L+ L+D +G   +   EF+R     +++ R   ++K F   D +  G++ + ++K  ++A KHPK  +G   E    Q FL SFE   +  G VT+ EF DYY  +S  I ++  F  MM+N
Sbjct:  118 RLLRAVAEKLTQPPTAAEQAEQQLVQQVQGEVRRQLAQRASKTLMGMGRHFRTLDKDGHRLFEE---------FWEVVEKEVLGGLWNLLDVNGDGALDYGEFVRGFIGEMNETRKAYVRKVFHKFDPSKTGLVELNNMKKFFSAKKHPKVLSGRLKEHEAQQMFLSSFENC-EHRGEVTYAEFEDYYEGVSIGIASDEEFVAMMKN 314          
BLAST of Calcyphosin-like protein vs. Ensembl Nematostella
Match: EDO33580 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRU7])

HSP 1 Score: 58.151 bits (139), Expect = 1.182e-10
Identity = 43/145 (29.66%), Postives = 69/145 (47.59%), Query Frame = 2
Query:  218 FRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLK-LIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNS 649
            F + + D D  ++  E    ++  G   TD EI ++ K +D DGS +I   EFL+ +         +  +  AF + DK G+G IT+ +LK V ++           SE  V  E +K  EA +D DG V  +EF+    +I+  
Sbjct:   17 FSLFDKDGDGTVTTAELGTVMRNLGQNPTDEEIREMIKEVDEDGSGSIGFEEFLQLMSKKTKGKSYEDELMAAFQIFDKDGNGSITVTELKEVLDSLGEK------LSEDEV-GEMIK--EADSDGDGTVNIEEFIKMMVAITGG 152          
BLAST of Calcyphosin-like protein vs. Ensembl Medaka
Match: capslb (calcyphosine like [Source:NCBI gene;Acc:101171382])

HSP 1 Score: 235.728 bits (600), Expect = 3.459e-77
Identity = 115/209 (55.02%), Postives = 151/209 (72.25%), Query Frame = 2
Query:   74 TMAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
             MA + + D E+ + A+++LS   DP+ KLRLQCL  G+SGIKGL R FR+M+D+ +R L   EF  G+  +GLL+   E   LF+  DRDGS TI   EFL ALRPP+S  RL+++ +AF  MDKTGDGVIT+EDL+ VYNA  HPK QNGE SE +VF+ FL SF++P D DG VT +EF+DYYS +S SID++ YF LMMRN++K+
Sbjct:   50 AMAGTSRHDHEMAVMAKRQLSQCSDPVEKLRLQCLIRGSSGIKGLGRTFRVMDDNQNRSLDLKEFLKGLNDYGLLMEKEEAAALFQHFDRDGSGTISFDEFLIALRPPMSRARLEVVMQAFRKMDKTGDGVITVEDLRGVYNAKYHPKYQNGELSEDQVFRTFLDSFDSPYDKDGKVTKEEFIDYYSGVSASIDSDVYFILMMRNAWKL 258          
BLAST of Calcyphosin-like protein vs. Ensembl Medaka
Match: calm2a (calmodulin 2 [Source:NCBI gene;Acc:100049420])

HSP 1 Score: 55.8398 bits (133), Expect = 2.408e-9
Identity = 40/148 (27.03%), Postives = 71/148 (47.97%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            I      F + + D D  ++  E    ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+   ++
Sbjct:   10 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVM-------TNLGEKLTDEEVDEMIR--EADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calcyphosin-like protein vs. Ensembl Medaka
Match: ENSORLT00000008599.2 (calmodulin 3 [Source:NCBI gene;Acc:100049421])

HSP 1 Score: 55.8398 bits (133), Expect = 2.408e-9
Identity = 40/148 (27.03%), Postives = 71/148 (47.97%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            I      F + + D D  ++  E    ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+   ++
Sbjct:   10 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVM-------TNLGEKLTDEEVDEMIR--EADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calcyphosin-like protein vs. Ensembl Medaka
Match: calm1b (calmodulin [Source:NCBI gene;Acc:100049419])

HSP 1 Score: 55.8398 bits (133), Expect = 2.408e-9
Identity = 40/148 (27.03%), Postives = 71/148 (47.97%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            I      F + + D D  ++  E    ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+   ++
Sbjct:   10 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVM-------TNLGEKLTDEEVDEMIR--EADIDGDGQVNYEEFVQMMTA 148          
BLAST of Calcyphosin-like protein vs. Ensembl Medaka
Match: ENSORLT00000022188.2 (calmodulin [Source:NCBI gene;Acc:100049422])

HSP 1 Score: 55.4546 bits (132), Expect = 4.104e-9
Identity = 40/148 (27.03%), Postives = 71/148 (47.97%), Query Frame = 2
Query:  197 IKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSD-NRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSS 637
            I      F + + D D  ++  E    ++  G   T++E++D+   +D DG+ TI   EFL  +   + D +  + I++AF + DK G+G I+  +L+HV           GE        E ++  EA  D DG V ++EF+   ++
Sbjct:   24 IAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNL-------GEKLTDEEVDEMIR--EADIDGDGQVNYEEFVQMMTA 162          
BLAST of Calcyphosin-like protein vs. Planmine SMEST
Match: SMESG000017753.1 (SMESG000017753.1)

HSP 1 Score: 428.328 bits (1100), Expect = 5.400e-154
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM
Sbjct:    1 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 208          
BLAST of Calcyphosin-like protein vs. Planmine SMEST
Match: SMESG000005291.1 (SMESG000005291.1)

HSP 1 Score: 247.284 bits (630), Expect = 1.480e-82
Identity = 114/208 (54.81%), Postives = 159/208 (76.44%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MAF+ +++ EL++++ K+L  A DP+ +LRL CL  GA+GIKGLSR F++ +DD  R L   EFK GI  +G+++ ++EI ++F  +DRD S T+   EFL ALRPP+S +R+++I KAF  MDKT DG IT+EDLK VY+A  HPK ++GEW+E +VF+EFLK+FEAP + DGIVT +EFL+YY  IS SIDN+ YFDLMMRN++K+
Sbjct:    1 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKVFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMSKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 208          
BLAST of Calcyphosin-like protein vs. Planmine SMEST
Match: SMESG000005291.1 (SMESG000005291.1)

HSP 1 Score: 247.284 bits (630), Expect = 3.240e-82
Identity = 114/208 (54.81%), Postives = 159/208 (76.44%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MAF+ +++ EL++++ K+L  A DP+ +LRL CL  GA+GIKGLSR F++ +DD  R L   EFK GI  +G+++ ++EI ++F  +DRD S T+   EFL ALRPP+S +R+++I KAF  MDKT DG IT+EDLK VY+A  HPK ++GEW+E +VF+EFLK+FEAP + DGIVT +EFL+YY  IS SIDN+ YFDLMMRN++K+
Sbjct:    1 MAFTSRENKELQVQSNKKLQNATDPVERLRLMCLSRGATGIKGLSRTFKVFDDDGSRSLDFNEFKKGIADYGIVMDNNEIHEIFNQIDRDKSGTLDFDEFLIALRPPMSKSRVEMISKAFAKMDKTKDGAITVEDLKGVYSAVHHPKFKSGEWTEDQVFREFLKNFEAPEEIDGIVTKEEFLNYYCGISASIDNDAYFDLMMRNAYKL 208          
BLAST of Calcyphosin-like protein vs. Planmine SMEST
Match: SMESG000025859.1 (SMESG000025859.1)

HSP 1 Score: 226.868 bits (577), Expect = 1.631e-74
Identity = 107/208 (51.44%), Postives = 154/208 (74.04%), Query Frame = 2
Query:   77 MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRIMNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEFLRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFKM 700
            MA +++ + EL+MK++++L +A DP+ KLRLQCL  GA+GIKGL R F+I +DD +  L   EF  G+  +G  L+  E++ +F+ +D+D S T+   EFL ALRPP++ +R+ L+ +AF  +DKT DG IT+EDLK VYN +KHPK  +GE +E  +F EFLK+FEAP++ DG VT +EFL+YYS +S SIDN+ YFDLMMRN++K+
Sbjct:    1 MALTNRGNRELQMKSKEKLKSASDPMEKLRLQCLARGATGIKGLGRAFKIYDDDGNNTLDFREFNKGVNDYGCDLSKEEVQFIFEGLDKDKSGTLDFDEFLIALRPPMTKSRVALVIQAFNKLDKTKDGFITVEDLKGVYNVSKHPKYISGERTEEELFLEFLKNFEAPDEIDGKVTKEEFLNYYSGVSASIDNDAYFDLMMRNAYKL 208          
BLAST of Calcyphosin-like protein vs. Planmine SMEST
Match: SMESG000072346.1 (SMESG000072346.1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.183e-24
Identity = 45/75 (60.00%), Postives = 58/75 (77.33%), Query Frame = 2
Query:  473 IEDLKHVYNATKHPKCQNGEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDLMMRNSFK 697
            I+D+K VY+  K+ +  NG+W+E + F EFLK+FE  N  DGIVT++EFLDYYS IS SID + YF+LMMRNSFK
Sbjct:   15 IDDVKGVYDIRKNSRYLNGDWTEEKCFLEFLKNFENQNQIDGIVTYEEFLDYYSVISASIDKDSYFNLMMRNSFK 89          
The following BLAST results are available for this feature:
BLAST of Calcyphosin-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CAPSL1.380e-7352.40calcyphosine like [Source:HGNC Symbol;Acc:HGNC:283... [more]
CAPSL1.380e-7352.40calcyphosine like [Source:HGNC Symbol;Acc:HGNC:283... [more]
CAPSL1.380e-7352.40calcyphosine like [Source:HGNC Symbol;Acc:HGNC:283... [more]
CAPSL8.490e-6752.91calcyphosine like [Source:HGNC Symbol;Acc:HGNC:283... [more]
CAPSL1.028e-6053.11calcyphosine like [Source:HGNC Symbol;Acc:HGNC:283... [more]
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BLAST of Calcyphosin-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cal-81.426e-929.08CALmodulin related genes [Source:UniProtKB/TrEMBL... [more]
cmd-12.855e-927.03Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O1630... [more]
cal-23.320e-825.95CALmodulin related genes; Calmodulin-like protein ... [more]
cmd-15.614e-830.17Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O1630... [more]
cal-49.058e-825.15CALmodulin related genes [Source:UniProtKB/TrEMBL... [more]
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BLAST of Calcyphosin-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG313453.205e-7354.15gene:FBgn0051345 transcript:FBtr0082689[more]
CG101261.178e-6250.00gene:FBgn0038088 transcript:FBtr0082686[more]
CG101263.665e-6250.00gene:FBgn0038088 transcript:FBtr0082687[more]
Cam1.587e-927.70gene:FBgn0000253 transcript:FBtr0304964[more]
Cam1.587e-927.70gene:FBgn0000253 transcript:FBtr0088001[more]
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BLAST of Calcyphosin-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
capslb1.647e-7250.24calcyphosine-like b [Source:ZFIN;Acc:ZDB-GENE-0504... [more]
capsla3.148e-6648.66calcyphosine-like a [Source:ZFIN;Acc:ZDB-GENE-0301... [more]
calm2a2.749e-927.03calmodulin 2a (phosphorylase kinase, delta) [Sourc... [more]
calm3a2.859e-927.03calmodulin 3a (phosphorylase kinase, delta) [Sourc... [more]
calm3b2.859e-927.03calmodulin 3b (phosphorylase kinase, delta) [Sourc... [more]
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BLAST of Calcyphosin-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
capsl1.170e-7551.92calcyphosine like [Source:NCBI gene;Acc:549278][more]
capsl1.051e-7350.48calcyphosine like [Source:NCBI gene;Acc:549278][more]
capsl7.221e-7352.02calcyphosine like [Source:NCBI gene;Acc:549278][more]
caps1.496e-7050.96calcyphosine [Source:NCBI gene;Acc:100037881][more]
ENSXETT00000016513.11.076e-2129.41calcyphosine 2 [Source:NCBI gene;Acc:100380015][more]
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BLAST of Calcyphosin-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Capsl1.260e-7652.40calcyphosine-like [Source:MGI Symbol;Acc:MGI:19228... [more]
Capsl1.431e-2748.57calcyphosine-like [Source:MGI Symbol;Acc:MGI:19228... [more]
Caps21.861e-1625.14calcyphosphine 2 [Source:MGI Symbol;Acc:MGI:244198... [more]
Caps21.909e-1625.14calcyphosphine 2 [Source:MGI Symbol;Acc:MGI:244198... [more]
Calm33.085e-927.03calmodulin 3 [Source:MGI Symbol;Acc:MGI:103249][more]
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BLAST of Calcyphosin-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6P8Y1|CAPSL_MOUSE8.813e-7652.40Calcyphosin-like protein OS=Mus musculus OX=10090 ... [more]
sp|Q8WWF8|CAPSL_HUMAN6.629e-7352.40Calcyphosin-like protein OS=Homo sapiens OX=9606 G... [more]
sp|Q0VCC0|CAYP1_BOVIN2.422e-5344.26Calcyphosin OS=Bos taurus OX=9913 GN=CAPS PE=2 SV=... [more]
sp|P41150|CAYP1_RABIT3.293e-5243.16Calcyphosin OS=Oryctolagus cuniculus OX=9986 GN=CA... [more]
sp|Q13938|CAYP1_HUMAN4.448e-5242.56Calcyphosin OS=Homo sapiens OX=9606 GN=CAPS PE=1 S... [more]
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BLAST of Calcyphosin-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8HIY94.974e-9262.50Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267H6P06.836e-7855.24Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A437CVX02.286e-7656.25Uncharacterized protein OS=Oryzias javanicus OX=12... [more]
A0A1I8I1U96.164e-7654.03Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267F2D98.839e-7655.98Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Calcyphosin-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
capslb1.078e-7150.00calcyphosine like [Source:NCBI gene;Acc:103021302][more]
capslb2.492e-7150.48calcyphosine like [Source:NCBI gene;Acc:103021302][more]
capsla1.254e-6449.19calcyphosin-like protein [Source:NCBI gene;Acc:103... [more]
calm3a2.423e-927.03calmodulin [Source:NCBI gene;Acc:103037741][more]
calm2a2.423e-927.03calmodulin [Source:NCBI gene;Acc:103037741][more]
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BLAST of Calcyphosin-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 4
Match NameE-valueIdentityDescription
CALML34.725e-1027.03calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:145... [more]
CALM34.915e-1027.03calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449][more]
ENSPMAT00000006008.17.424e-1027.03pep scaffold:Pmarinus_7.0:GL476713:142259:146509:-... [more]
si:rp71-17i16.46.331e-626.35si:rp71-17i16.4 [Source:ZFIN;Acc:ZDB-GENE-090312-2... [more]
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BLAST of Calcyphosin-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
CMD15.547e-1131.07Calmodulin; Ca2+ binding protein that regulates Ca... [more]
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BLAST of Calcyphosin-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO329611.969e-7551.92Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO329623.162e-4949.12Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO337232.746e-3543.83Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO377274.020e-1825.60Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO335801.182e-1029.66Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Calcyphosin-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
capslb3.459e-7755.02calcyphosine like [Source:NCBI gene;Acc:101171382][more]
calm2a2.408e-927.03calmodulin 2 [Source:NCBI gene;Acc:100049420][more]
ENSORLT00000008599.22.408e-927.03calmodulin 3 [Source:NCBI gene;Acc:100049421][more]
calm1b2.408e-927.03calmodulin [Source:NCBI gene;Acc:100049419][more]
ENSORLT00000022188.24.104e-927.03calmodulin [Source:NCBI gene;Acc:100049422][more]
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BLAST of Calcyphosin-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000017753.15.400e-154100.00SMESG000017753.1[more]
SMESG000005291.11.480e-8254.81SMESG000005291.1[more]
SMESG000005291.13.240e-8254.81SMESG000005291.1[more]
SMESG000025859.11.631e-7451.44SMESG000025859.1[more]
SMESG000072346.11.183e-2460.00SMESG000072346.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001720 ID=SMED30001720|Name=Calcyphosin-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=846bp
GCTAATTGTATTTACTCTATTGCCGTCGCATTTATTATTTGTTTTTAAGT
ATTCATAGGACATTATTAAATTGACAATGGCTTTCAGTGACAAAGATGAT
ATGGAACTAAGAATGAAAGCTCGCAAAGAACTTTCAACTGCTAAAGATCC
TCTGACAAAATTAAGATTACAATGCTTATGCAATGGAGCCAGTGGAATCA
AAGGATTATCACGACGATTTCGCATAATGAATGATGATAATGATAGGTTT
TTATCTCAGACAGAATTTAAAAATGGTATCAAAGGATTTGGACTGCTGTT
AACTGATTCAGAAATCGAAGATTTATTCAAGTTAATGGATCGAGATGGAA
GTAACACAATTTGTATGACTGAATTTCTGCGTGCTTTAAGACCACCGTTG
TCGGATAACCGATTGAAATTGATCAAAAAGGCCTTTACTTTGATGGATAA
AACTGGAGATGGAGTAATAACAATCGAAGATCTAAAGCATGTATATAATG
CAACGAAGCATCCAAAATGTCAAAATGGTGAATGGTCTGAAACTCGGGTT
TTTCAGGAATTTCTCAAAAGTTTTGAGGCGCCGAATGATGCCGACGGAAT
AGTTACGTGGGATGAATTTCTGGATTATTATTCCTCAATCAGCAATTCCA
TAGATAATGAAGGCTATTTTGATCTGATGATGCGCAATTCGTTCAAAATG
TGAGATGATGCAATAAGGATTTTTGATATCCATTTAGTTATATTTTTCTA
ATTTTACTAGTCTTTGGTAGATGGTTTTATTTAATCTATTGAGCTTATAT
AAAGTTTTTTTATTTCAATTAAATTTTATATTACTCTCAAAAAAAA
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protein sequence of SMED30001720-orf-1

>SMED30001720-orf-1 ID=SMED30001720-orf-1|Name=SMED30001720-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=209bp
MAFSDKDDMELRMKARKELSTAKDPLTKLRLQCLCNGASGIKGLSRRFRI
MNDDNDRFLSQTEFKNGIKGFGLLLTDSEIEDLFKLMDRDGSNTICMTEF
LRALRPPLSDNRLKLIKKAFTLMDKTGDGVITIEDLKHVYNATKHPKCQN
GEWSETRVFQEFLKSFEAPNDADGIVTWDEFLDYYSSISNSIDNEGYFDL
MMRNSFKM*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000484secretory system
PLANA:0003116parenchymal cell
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR029633CAPSL
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
GO:0046872metal ion binding
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 115..143
e-value: 0.085
score: 22.0
coord: 43..71
e-value: 41.0
score: 4.8
coord: 79..107
e-value: 0.014
score: 24.6
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 45..104
e-value: 9.8E-8
score: 32.3
coord: 116..185
e-value: 1.2E-7
score: 32.1
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 39..74
score: 8.544
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 171..191
score: 6.647
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 111..146
score: 11.668
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 75..110
score: 11.668
IPR002048EF-hand domainCDDcd00051EFhcoord: 44..105
e-value: 3.09487E-10
score: 52.1649
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 23..108
e-value: 2.9E-17
score: 64.2
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 109..208
e-value: 7.9E-33
score: 115.0
NoneNo IPR availablePANTHERPTHR34524FAMILY NOT NAMEDcoord: 1..208
IPR029633Calcyphosin-like proteinPANTHERPTHR34524:SF5CALCYPHOSIN-LIKE PROTEINcoord: 1..208
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 88..100
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 41..204