Vacuolar protein sorting 4

Overview
NameVacuolar protein sorting 4
Smed IDSMED30001710
Length (bp)1802
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Vacuolar protein sorting 4 (SMED30001710) t-SNE clustered cells

Violin plots show distribution of expression levels for Vacuolar protein sorting 4 (SMED30001710) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Vacuolar protein sorting 4 (SMED30001710) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Vacuolar protein sorting 4 (SMED30001710) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30001710

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 18

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30001710h1SMcG0014813 Contig1083GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30001710h1SMcG0014813 Contig1087GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30001710h1SMcG0014813 Contig1087GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30001710h1SMcG0018316 Contig1083GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30001710h1SMcG0016465 Contig1087GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30001710h1SMcG0016465 Contig1087GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30001710h1SMcG0014813 dd_Smed_v4_154_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30001710h1SMcG0014813 dd_Smed_v4_154_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30001710h1SMcG0014813 dd_Smed_v4_154_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30001710h1SMcG0014813 dd_Smed_v4_154_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001710h1SMcG0014813 Contig48792uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001710h1SMcG0014813 Contig48792newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
protonephridiaSMED30001710h1SMcG0014813 dd_Smed_v4_1556_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30001710h1SMcG0014813 dd_Smed_v4_1556_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30001710h1SMcG0014813 dd_Smed_v6_154_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
goblet cellSMED30001710h1SMcG0014813 dd_Smed_v6_154_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30001710h1SMcG0014813 dd_Smed_v6_154_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30001710h1SMcG0014813 dd_Smed_v4_1556_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Vacuolar protein sorting 4 vs. Ensembl Human
Match: VPS4A (vacuolar protein sorting 4 homolog A [Source:HGNC Symbol;Acc:HGNC:13488])

HSP 1 Score: 575.474 bits (1482), Expect = 0.000e+0
Identity = 295/441 (66.89%), Postives = 362/441 (82.09%), Query Frame = 2
Query:   35 MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            M ++T+QKAI++VTKATEEDK KN++EAL+LY+H VEYFLH+IKY++HS+K+K+SIRAKC+QYL+RAEKLK++L +    K+KH K    E  N  +  G        + EKKKLQ QL  A+V+EKPN+ W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+L+SKWLGESEKLVKNLF LAR+ KPSIIFIDE+DSLC +R +N+SE+A+RIKTEFLVQMQGV ++ND  L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR +MF+L +G+ P  H LT  +  EL +++EGYSGADISI VRD+LM PVRKVQ+ATHFK+  GPS  NP +++DDLLTPCSPGDP A+ M W  + G+KLLEP V ++DM+++LA  +PTVN  DL K+ +F+ DF
Sbjct:    1 MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCVQYLDRAEKLKDYLRS----KEKHGKKPVKE--NQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVMEKPNIRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGSTP--HNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQSATHFKKVCGPSRTNPSMMIDDLLTPCSPGDPGAMEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNADDLLKVKKFSEDF 433          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Human
Match: VPS4B (vacuolar protein sorting 4 homolog B [Source:HGNC Symbol;Acc:HGNC:10895])

HSP 1 Score: 561.222 bits (1445), Expect = 0.000e+0
Identity = 298/447 (66.67%), Postives = 359/447 (80.31%), Query Frame = 2
Query:   35 MASTT--IQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEK----SLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            M+ST+  +QKAI++ +KA +EDK  N++EAL+LY+H V+YFLH +KY++  +K+K SIRAKC +YL+RAEKLKE+L  +    +K  K    S A E  ND    G+SD   +K      LQ+QL  AIV+E+PNV W DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFS+SSS+LVSKWLGESEKLVKNLF LARE KPSIIFIDEIDSLC +R +N+SE+A+RIKTEFLVQMQGV  DND +L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR  MFKL +G    Q++LT+ DF ELG++++GYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  +P  +VDDLLTPCSPGDP AI M W  + G+KLLEP V+++DM+++L+  KPTVN+ DL KL +FT DF
Sbjct:    1 MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKK-----LQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTT--QNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDF 440          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Human
Match: AC026464.4 (novel protein)

HSP 1 Score: 534.258 bits (1375), Expect = 0.000e+0
Identity = 278/405 (68.64%), Postives = 334/405 (82.47%), Query Frame = 2
Query:   56 KAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTL 1270
            KAI++VTKATEEDK KN++EAL+LY+H VEYFLH+IKY++HS+K+K+SIRAKC+QYL+RAEKLK++L +    K+KH K    E  N  +  G        + EKKKLQ QL  A+V+EKPN+ W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+L+SKWLGESEKLVKNLF LAR+ KPSIIFIDE+DSLC +R +N+SE+A+RIKTEFLVQMQGV ++ND  L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR +MF+L +G+ P  H LT  +  EL +++EGYSGADISI VRD+LM PVRKVQ+ATHFK+  GPS  NP +++DDLLTPCSPGDP A+ M W  + G+KLLEP V +
Sbjct:   32 KAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCVQYLDRAEKLKDYLRS----KEKHGKKPVKE--NQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVMEKPNIRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGSTP--HNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQSATHFKKVCGPSRTNPSMMIDDLLTPCSPGDPGAMEMTWMDVPGDKLLEPVVCM 428          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Human
Match: KATNA1 (katanin catalytic subunit A1 [Source:HGNC Symbol;Acc:HGNC:6216])

HSP 1 Score: 229.95 bits (585), Expect = 8.000e-68
Identity = 129/249 (51.81%), Postives = 169/249 (67.87%), Query Frame = 2
Query:  377 LQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDS-ESAKRIKTEFLVQMQGV--SSDNDN----VLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRK 1102
            L   L   I+ + PNV WDD+A L+ AK+ LKEAV+LP+  P  F G R PWKG+L+ GPPGTGK+ LAKAVATE   +TFF+VSSS L SK+ GESEKLV+ LF +AR   P+ IFIDEIDS+CS RG ++  E+++R+K E LVQM GV  +S+ND+    V++LAATN PW +D A+RRR EKRIYI LP A  R E+ ++S+            D   + +  EGYSGADI+   RDA +  +R+
Sbjct:  191 LVEALERDIISQNPNVRWDDIADLVEAKKLLKEAVVLPMWMPEFFKGIRRPWKGVLMVGPPGTGKTLLAKAVATEC-KTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYSPATIFIDEIDSICSRRGTSEEHEASRRVKAELLVQMDGVGGTSENDDPSKMVMVLAATNFPWDIDEALRRRLEKRIYIPLPSAKGREELLRISLRE---LELADDVDLASIAENMEGYSGADITNVCRDASLMAMRR 435          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Human
Match: KATNA1 (katanin catalytic subunit A1 [Source:HGNC Symbol;Acc:HGNC:6216])

HSP 1 Score: 229.95 bits (585), Expect = 8.000e-68
Identity = 129/249 (51.81%), Postives = 169/249 (67.87%), Query Frame = 2
Query:  377 LQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDS-ESAKRIKTEFLVQMQGV--SSDNDN----VLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRK 1102
            L   L   I+ + PNV WDD+A L+ AK+ LKEAV+LP+  P  F G R PWKG+L+ GPPGTGK+ LAKAVATE   +TFF+VSSS L SK+ GESEKLV+ LF +AR   P+ IFIDEIDS+CS RG ++  E+++R+K E LVQM GV  +S+ND+    V++LAATN PW +D A+RRR EKRIYI LP A  R E+ ++S+            D   + +  EGYSGADI+   RDA +  +R+
Sbjct:  191 LVEALERDIISQNPNVRWDDIADLVEAKKLLKEAVVLPMWMPEFFKGIRRPWKGVLMVGPPGTGKTLLAKAVATEC-KTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYSPATIFIDEIDSICSRRGTSEEHEASRRVKAELLVQMDGVGGTSENDDPSKMVMVLAATNFPWDIDEALRRRLEKRIYIPLPSAKGREELLRISLRE---LELADDVDLASIAENMEGYSGADITNVCRDASLMAMRR 435          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Celegans
Match: vps-4 (Related to yeast Vacuolar Protein Sorting factor [Source:UniProtKB/TrEMBL;Acc:Q9BL83])

HSP 1 Score: 517.309 bits (1331), Expect = 1.685e-180
Identity = 277/441 (62.81%), Postives = 335/441 (75.96%), Query Frame = 2
Query:   35 MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            M+   +QKAIE+VTKATEED    + +AL+LY+  +EYFLH+IKY+S  +K +++IR K  QYL RAE++K  L                +G   KK   D   DS++D +KKK Q +L+ AIV+EKPNV W D+AGL  AKEALKEAVILPIKFP LFTG R PW+GILL+GPPGTGKSY+AKAVATEA  STFFS+SSS+L+SKWLGESEKLVKNLF LARE KPSIIFIDEIDSLCS R DN+SESA+RIKTEF+VQMQGV  +ND +L+L ATNIPW LDSAIRRRFEKRIYI LP+  AR EMF++ +G     +TLT QDF  L +R EGYSG DISI V+DALM PVR+VQ+ATHFK   GPSP++P +I  DLLTPCSPGDP AIAMNW  + G+KL  P +++ D+ ++LA  KPTVN  DL++L  F NDF
Sbjct:    1 MSVPALQKAIELVTKATEEDTAGRYDQALRLYDQAIEYFLHAIKYESQGDKQRNAIRDKVGQYLNRAEQIKTHLK---------------DGKTQKKPVKDGKDDSDEDEDKKKFQDKLSGAIVMEKPNVKWTDIAGLEGAKEALKEAVILPIKFPQLFTGNRKPWQGILLFGPPGTGKSYIAKAVATEAGESTFFSISSSDLMSKWLGESEKLVKNLFALAREHKPSIIFIDEIDSLCSARSDNESESARRIKTEFMVQMQGVGLNNDGILVLGATNIPWILDSAIRRRFEKRIYIPLPDIHARKEMFRIDVGK--NYNTLTDQDFKVLAERCEGYSGYDISILVKDALMQPVRRVQSATHFKHVSGPSPKDPNVIAHDLLTPCSPGDPHAIAMNWLDVPGDKLANPPLSMQDISRSLASVKPTVNNTDLDRLEAFKNDF 424          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Celegans
Match: spas-1 (Probable spastin homolog spas-1 [Source:UniProtKB/Swiss-Prot;Acc:Q8MNV0])

HSP 1 Score: 204.527 bits (519), Expect = 4.715e-59
Identity = 123/319 (38.56%), Postives = 176/319 (55.17%), Query Frame = 2
Query:  404 VVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQG-VSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            V++   V  DDVAG  +AK AL+EAVILP   P+LF G R P KGILL+GPPG GK+ LAKAVA E+    F   +SS    KW+G+SEK ++ LF +AR  +PSIIFIDEIDS+   R + D+E ++R+KTEFLVQ  G  SS +D +L++ ATN P  LD A+ RRF KRI + LP+  AR E+   ++        L   D   +   + G+S +D+    ++A M P+R++                               D   ++M     +GEK+ +  +  +D   AL   +P+ +QK + KL  F+  F
Sbjct:  169 VLDNTGVRMDDVAGCHSAKAALEEAVILPALNPNLFKGLRQPVKGILLFGPPGNGKTLLAKAVAGESKQMFFNISASSLTS-KWVGDSEKTIRGLFQIARNAQPSIIFIDEIDSILCERSEKDAEVSRRMKTEFLVQFDGATSSADDRILVIGATNRPHELDDAVLRRFPKRIMLNLPDEEARKELITKTLKKHNMMDGLISSDIRYIASNTSGFSNSDLVALCKEAAMVPIREI-------------------------------DRSKLSMT----DGEKIRK--IRASDFDTALRTIRPSTSQKIMSKLSDFSRSF 449          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Celegans
Match: spas-1 (Probable spastin homolog spas-1 [Source:UniProtKB/Swiss-Prot;Acc:Q8MNV0])

HSP 1 Score: 204.527 bits (519), Expect = 1.444e-58
Identity = 123/319 (38.56%), Postives = 176/319 (55.17%), Query Frame = 2
Query:  404 VVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQG-VSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            V++   V  DDVAG  +AK AL+EAVILP   P+LF G R P KGILL+GPPG GK+ LAKAVA E+    F   +SS    KW+G+SEK ++ LF +AR  +PSIIFIDEIDS+   R + D+E ++R+KTEFLVQ  G  SS +D +L++ ATN P  LD A+ RRF KRI + LP+  AR E+   ++        L   D   +   + G+S +D+    ++A M P+R++                               D   ++M     +GEK+ +  +  +D   AL   +P+ +QK + KL  F+  F
Sbjct:  230 VLDNTGVRMDDVAGCHSAKAALEEAVILPALNPNLFKGLRQPVKGILLFGPPGNGKTLLAKAVAGESKQMFFNISASSLTS-KWVGDSEKTIRGLFQIARNAQPSIIFIDEIDSILCERSEKDAEVSRRMKTEFLVQFDGATSSADDRILVIGATNRPHELDDAVLRRFPKRIMLNLPDEEARKELITKTLKKHNMMDGLISSDIRYIASNTSGFSNSDLVALCKEAAMVPIREI-------------------------------DRSKLSMT----DGEKIRK--IRASDFDTALRTIRPSTSQKIMSKLSDFSRSF 510          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Celegans
Match: mei-1 (Meiotic spindle formation protein mei-1 [Source:UniProtKB/Swiss-Prot;Acc:P34808])

HSP 1 Score: 203.371 bits (516), Expect = 1.546e-58
Identity = 115/230 (50.00%), Postives = 161/230 (70.00%), Query Frame = 2
Query:  431 DDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDN-DSESAKRIKTEFLVQMQGVSS--DNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQT 1111
            DD+ G+ + K+ L EAV LP+  P  F G R+PWK ++L GPPGTGK+ +A+A+A+E+ +STFF+VSS++L SKW G+SEK+V+ LF LAR   PSIIFIDEID+L   RG++ + E+++R+K+EFLVQM G  +  D+  V +LAATNIPW LD A+RRRFEKRI+I LP+  AR ++ + SM   P    +   D   L  R+EG+SGAD+    R A +  +R+  T
Sbjct:  193 DDIIGMHDVKQVLHEAVTLPLLVPEFFQGLRSPWKAMVLAGPPGTGKTLIARAIASES-SSTFFTVSSTDLSSKWRGDSEKIVRLLFELARFYAPSIIFIDEIDTLGGQRGNSGEHEASRRVKSEFLVQMDGSQNKFDSRRVFVLAATNIPWELDEALRRRFEKRIFIPLPDIDARKKLIEKSMEGTPKSDEINYDD---LAARTEGFSGADVVSLCRTAAINVLRRYDT 418          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Celegans
Match: mei-1 (Meiotic spindle formation protein mei-1 [Source:UniProtKB/Swiss-Prot;Acc:P34808])

HSP 1 Score: 202.216 bits (513), Expect = 5.689e-58
Identity = 114/227 (50.22%), Postives = 160/227 (70.48%), Query Frame = 2
Query:  431 DDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDN-DSESAKRIKTEFLVQMQGVSS--DNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRK 1102
            DD+ G+ + K+ L EAV LP+  P  F G R+PWK ++L GPPGTGK+ +A+A+A+E+ +STFF+VSS++L SKW G+SEK+V+ LF LAR   PSIIFIDEID+L   RG++ + E+++R+K+EFLVQM G  +  D+  V +LAATNIPW LD A+RRRFEKRI+I LP+  AR ++ + SM   P    +   D   L  R+EG+SGAD+    R A +  +R+
Sbjct:  193 DDIIGMHDVKQVLHEAVTLPLLVPEFFQGLRSPWKAMVLAGPPGTGKTLIARAIASES-SSTFFTVSSTDLSSKWRGDSEKIVRLLFELARFYAPSIIFIDEIDTLGGQRGNSGEHEASRRVKSEFLVQMDGSQNKFDSRRVFVLAATNIPWELDEALRRRFEKRIFIPLPDIDARKKLIEKSMEGTPKSDEINYDD---LAARTEGFSGADVVSLCRTAAINVLRR 415          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Fly
Match: Vps4 (gene:FBgn0283469 transcript:FBtr0074504)

HSP 1 Score: 565.459 bits (1456), Expect = 0.000e+0
Identity = 310/440 (70.45%), Postives = 356/440 (80.91%), Query Frame = 2
Query:   38 ASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            A TT+QKAI++VTKATEED+NKN+ EAL+LYEHGVEYFLH+IKY++  EK+KDSIRAKC+QYL+RAEKLKE+L        K     +    +DK K  DSD +   D EKKKLQS+L DAIV+EKP V W DVAGL  AKEALKEAVILPIKFP LFTGKR PWKGILL+GPPGTGKSYLAKAVATEAN STFFSVSSS+L+SKWLGESEKLVKNLF LAR+ KPSIIFIDEIDS+CS R DN+++S +RIKTEFLVQMQGV +D D +L+L ATNIPW LDSAIRRRFEKRIYI LPEA AR  MFK+ +GN    H LT+QD  EL  ++EGYSGADISI VRDALM PVRKVQTATHFKR  GPSP N E IV+DLL PCSPGD  A+ MNW  +  +KL EP VT+ DM+K+L+R KPTVN+ DL+KL +FT DF
Sbjct:    3 AGTTLQKAIDLVTKATEEDRNKNYAEALRLYEHGVEYFLHTIKYEAQGEKAKDSIRAKCLQYLDRAEKLKEYLKKGKKKPIKEGGESSA--KDDKDKKSDSDDEDGDDPEKKKLQSKLEDAIVIEKPKVQWSDVAGLDAAKEALKEAVILPIKFPQLFTGKRIPWKGILLFGPPGTGKSYLAKAVATEANRSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEIDSMCSARSDNENDSVRRIKTEFLVQMQGVGNDTDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEAHARLVMFKIHLGNT--THVLTEQDLKELAGKTEGYSGADISIVVRDALMEPVRKVQTATHFKRVSGPSPTNHEEIVNDLLVPCSPGDQGAVEMNWMDVPSDKLFEPPVTMRDMLKSLSRTKPTVNEDDLKKLRKFTEDF 438          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Fly
Match: Vps4 (gene:FBgn0283469 transcript:FBtr0342787)

HSP 1 Score: 565.459 bits (1456), Expect = 0.000e+0
Identity = 310/440 (70.45%), Postives = 356/440 (80.91%), Query Frame = 2
Query:   38 ASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            A TT+QKAI++VTKATEED+NKN+ EAL+LYEHGVEYFLH+IKY++  EK+KDSIRAKC+QYL+RAEKLKE+L        K     +    +DK K  DSD +   D EKKKLQS+L DAIV+EKP V W DVAGL  AKEALKEAVILPIKFP LFTGKR PWKGILL+GPPGTGKSYLAKAVATEAN STFFSVSSS+L+SKWLGESEKLVKNLF LAR+ KPSIIFIDEIDS+CS R DN+++S +RIKTEFLVQMQGV +D D +L+L ATNIPW LDSAIRRRFEKRIYI LPEA AR  MFK+ +GN    H LT+QD  EL  ++EGYSGADISI VRDALM PVRKVQTATHFKR  GPSP N E IV+DLL PCSPGD  A+ MNW  +  +KL EP VT+ DM+K+L+R KPTVN+ DL+KL +FT DF
Sbjct:    3 AGTTLQKAIDLVTKATEEDRNKNYAEALRLYEHGVEYFLHTIKYEAQGEKAKDSIRAKCLQYLDRAEKLKEYLKKGKKKPIKEGGESSA--KDDKDKKSDSDDEDGDDPEKKKLQSKLEDAIVIEKPKVQWSDVAGLDAAKEALKEAVILPIKFPQLFTGKRIPWKGILLFGPPGTGKSYLAKAVATEANRSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEIDSMCSARSDNENDSVRRIKTEFLVQMQGVGNDTDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEAHARLVMFKIHLGNT--THVLTEQDLKELAGKTEGYSGADISIVVRDALMEPVRKVQTATHFKRVSGPSPTNHEEIVNDLLVPCSPGDQGAVEMNWMDVPSDKLFEPPVTMRDMLKSLSRTKPTVNEDDLKKLRKFTEDF 438          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Fly
Match: kat-60L1 (gene:FBgn0037375 transcript:FBtr0300739)

HSP 1 Score: 214.542 bits (545), Expect = 2.805e-61
Identity = 136/334 (40.72%), Postives = 194/334 (58.08%), Query Frame = 2
Query:  377 LQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRG-DNDSESAKRIKTEFLVQMQGVSS---DNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGN---KPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            L   L   I+   P + W DVAGL  AK  L+EAV+LP+  P  F G R PW+G+L+ GPPGTGK+ LAKAVATE   +TFF+VSSS L SK+ GESEKLV+ LF +AR   PS IFIDEID+LC++RG D++ E+++R K E L+QM G+++   +   +++LAATN PW +D A RRRFEKRIYI LP    R+ + KL + +    P  +T        +G   +GYSG+DIS   RDA M  +R++ +            R P+ I                    + +  E++ +P +TL D   A  R K +V+  D+ +  ++  ++
Sbjct:  308 LVDTLEKDILQRHPCIKWTDVAGLNEAKTILQEAVVLPVIMPEFFKGIRRPWRGVLMVGPPGTGKTMLAKAVATEC-GTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPSTIFIDEIDALCASRGSDSEHEASRRFKAELLIQMDGLNASMQEEKVIMVLAATNHPWDIDEAFRRRFEKRIYIPLPNEGTRSALLKLCLKDVCLSPSLNTGI------IGDELQGYSGSDISNVCRDASMMAMRRLISG-----------RTPDQI--------------------KQIRREEVDQP-ITLQDFQDARLRTKKSVSADDVARFEKWMEEY 602          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Fly
Match: kat-60L1 (gene:FBgn0037375 transcript:FBtr0078627)

HSP 1 Score: 214.542 bits (545), Expect = 2.805e-61
Identity = 136/334 (40.72%), Postives = 194/334 (58.08%), Query Frame = 2
Query:  377 LQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRG-DNDSESAKRIKTEFLVQMQGVSS---DNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGN---KPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            L   L   I+   P + W DVAGL  AK  L+EAV+LP+  P  F G R PW+G+L+ GPPGTGK+ LAKAVATE   +TFF+VSSS L SK+ GESEKLV+ LF +AR   PS IFIDEID+LC++RG D++ E+++R K E L+QM G+++   +   +++LAATN PW +D A RRRFEKRIYI LP    R+ + KL + +    P  +T        +G   +GYSG+DIS   RDA M  +R++ +            R P+ I                    + +  E++ +P +TL D   A  R K +V+  D+ +  ++  ++
Sbjct:  308 LVDTLEKDILQRHPCIKWTDVAGLNEAKTILQEAVVLPVIMPEFFKGIRRPWRGVLMVGPPGTGKTMLAKAVATEC-GTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPSTIFIDEIDALCASRGSDSEHEASRRFKAELLIQMDGLNASMQEEKVIMVLAATNHPWDIDEAFRRRFEKRIYIPLPNEGTRSALLKLCLKDVCLSPSLNTGI------IGDELQGYSGSDISNVCRDASMMAMRRLISG-----------RTPDQI--------------------KQIRREEVDQP-ITLQDFQDARLRTKKSVSADDVARFEKWMEEY 602          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Fly
Match: kat-60L1 (gene:FBgn0037375 transcript:FBtr0300741)

HSP 1 Score: 214.157 bits (544), Expect = 3.860e-61
Identity = 136/334 (40.72%), Postives = 194/334 (58.08%), Query Frame = 2
Query:  377 LQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRG-DNDSESAKRIKTEFLVQMQGVSS---DNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGN---KPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            L   L   I+   P + W DVAGL  AK  L+EAV+LP+  P  F G R PW+G+L+ GPPGTGK+ LAKAVATE   +TFF+VSSS L SK+ GESEKLV+ LF +AR   PS IFIDEID+LC++RG D++ E+++R K E L+QM G+++   +   +++LAATN PW +D A RRRFEKRIYI LP    R+ + KL + +    P  +T        +G   +GYSG+DIS   RDA M  +R++ +            R P+ I                    + +  E++ +P +TL D   A  R K +V+  D+ +  ++  ++
Sbjct:  312 LVDTLEKDILQRHPCIKWTDVAGLNEAKTILQEAVVLPVIMPEFFKGIRRPWRGVLMVGPPGTGKTMLAKAVATEC-GTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPSTIFIDEIDALCASRGSDSEHEASRRFKAELLIQMDGLNASMQEEKVIMVLAATNHPWDIDEAFRRRFEKRIYIPLPNEGTRSALLKLCLKDVCLSPSLNTGI------IGDELQGYSGSDISNVCRDASMMAMRRLISG-----------RTPDQI--------------------KQIRREEVDQP-ITLQDFQDARLRTKKSVSADDVARFEKWMEEY 606          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Zebrafish
Match: vps4b (vacuolar protein sorting 4 homolog B [Source:ZFIN;Acc:ZDB-GENE-040426-1235])

HSP 1 Score: 547.354 bits (1409), Expect = 0.000e+0
Identity = 289/441 (65.53%), Postives = 358/441 (81.18%), Query Frame = 2
Query:   38 ASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEK-SLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            A+  +QKAI++  KA++EDK +N++EAL+LY+H V+YFLH +KY++  +K+K SIRAKC +YL+RAEKLKE+L  +     K  K S + +  N+     D +            Q+QL+ AIV+EKPN+ W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFS+SSS+LVSKWLGESEKLVK+LF LARE KPSIIFIDEIDSLC +R +N+SE+A+RIKTEFLVQMQGV +DN+ +L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR+ MFKL++G  P  ++LT+ DF+ LGK+++GYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  +P +IVDDLLTPCSPGDP A  M W  + GEKLLEP V+++DM+++L+  KPTVN++DLEKL +FT DF
Sbjct:    3 ANNNLQKAIDLANKASQEDKAENYEEALRLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCAEYLDRAEKLKEYLKKKEKAPAKPVKESQSNDKGNESDGEEDPEKKK--------FQNQLSGAIVMEKPNIKWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKSLFTLAREHKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGNDNEGILVLGATNIPWTLDSAIRRRFEKRIYIPLPEEHARSFMFKLNLGTTP--NSLTESDFMTLGKKTDGYSGADISIIVRDALMQPVRKVQSATHFKQVRGPSRSDPNVIVDDLLTPCSPGDPQAKEMTWMEVPGEKLLEPIVSMSDMLRSLSNTKPTVNEQDLEKLKKFTEDF 433          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Zebrafish
Match: vps4b (vacuolar protein sorting 4 homolog B [Source:ZFIN;Acc:ZDB-GENE-040426-1235])

HSP 1 Score: 547.354 bits (1409), Expect = 0.000e+0
Identity = 289/441 (65.53%), Postives = 358/441 (81.18%), Query Frame = 2
Query:   38 ASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEK-SLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            A+  +QKAI++  KA++EDK +N++EAL+LY+H V+YFLH +KY++  +K+K SIRAKC +YL+RAEKLKE+L  +     K  K S + +  N+     D +            Q+QL+ AIV+EKPN+ W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFS+SSS+LVSKWLGESEKLVK+LF LARE KPSIIFIDEIDSLC +R +N+SE+A+RIKTEFLVQMQGV +DN+ +L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR+ MFKL++G  P  ++LT+ DF+ LGK+++GYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  +P +IVDDLLTPCSPGDP A  M W  + GEKLLEP V+++DM+++L+  KPTVN++DLEKL +FT DF
Sbjct:    3 ANNNLQKAIDLANKASQEDKAENYEEALRLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCAEYLDRAEKLKEYLKKKEKAPAKPVKESQSNDKGNESDGEEDPEKKK--------FQNQLSGAIVMEKPNIKWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKSLFTLAREHKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGNDNEGILVLGATNIPWTLDSAIRRRFEKRIYIPLPEEHARSFMFKLNLGTTP--NSLTESDFMTLGKKTDGYSGADISIIVRDALMQPVRKVQSATHFKQVRGPSRSDPNVIVDDLLTPCSPGDPQAKEMTWMEVPGEKLLEPIVSMSDMLRSLSNTKPTVNEQDLEKLKKFTEDF 433          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Zebrafish
Match: vps4a (vacuolar protein sorting 4 homolog A [Source:ZFIN;Acc:ZDB-GENE-060929-388])

HSP 1 Score: 546.969 bits (1408), Expect = 0.000e+0
Identity = 278/413 (67.31%), Postives = 342/413 (82.81%), Query Frame = 2
Query:   35 MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFL-SAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTL 1270
            M ++T+QKAIE+VTKATEEDK KN++EAL+LY+H VEYFLH+IKY++HS+K+K+SIR KC+QYL+RAEKLK++L + +  GKK  +++ + + ++   +  + +           LQ  L  AIV+EKPNV W DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+L+SKWLGESEKLVKNLF LAR+ KPSIIFIDE+DSLC +R +N+SE+A+RIKTEFLVQMQGV ++ND +L+L ATNIPW LD+AIRRRFEKRIYI LPE PAR+ MF+L +GN P  H+LT+ D  +L ++++GYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  N  +IVDDLLTPCSPGDP+AI M W  + G+KLLEP V +
Sbjct:    1 MTTSTLQKAIELVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRGKCMQYLDRAEKLKDYLKNKDKQGKKPVKETQSNDKSDSDSEGENPEKKK--------LQEHLMGAIVMEKPNVRWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFDLARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGILVLGATNIPWVLDAAIRRRFEKRIYIPLPEEPARSAMFRLHLGNTP--HSLTEADLRQLARKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRSNSAVIVDDLLTPCSPGDPEAIEMTWMDVPGDKLLEPIVCM 403          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Zebrafish
Match: vps4b (vacuolar protein sorting 4 homolog B [Source:ZFIN;Acc:ZDB-GENE-040426-1235])

HSP 1 Score: 543.502 bits (1399), Expect = 0.000e+0
Identity = 287/436 (65.83%), Postives = 355/436 (81.42%), Query Frame = 2
Query:   53 QKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEK-SLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            +KAI++  KA++EDK +N++EAL+LY+H V+YFLH +KY++  +K+K SIRAKC +YL+RAEKLKE+L  +     K  K S + +  N+     D +            Q+QL+ AIV+EKPN+ W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFS+SSS+LVSKWLGESEKLVK+LF LARE KPSIIFIDEIDSLC +R +N+SE+A+RIKTEFLVQMQGV +DN+ +L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR+ MFKL++G  P  ++LT+ DF+ LGK+++GYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  +P +IVDDLLTPCSPGDP A  M W  + GEKLLEP V+++DM+++L+  KPTVN++DLEKL +FT DF
Sbjct:   78 EKAIDLANKASQEDKAENYEEALRLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCAEYLDRAEKLKEYLKKKEKAPAKPVKESQSNDKGNESDGEEDPEKKK--------FQNQLSGAIVMEKPNIKWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKSLFTLAREHKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGNDNEGILVLGATNIPWTLDSAIRRRFEKRIYIPLPEEHARSFMFKLNLGTTP--NSLTESDFMTLGKKTDGYSGADISIIVRDALMQPVRKVQSATHFKQVRGPSRSDPNVIVDDLLTPCSPGDPQAKEMTWMEVPGEKLLEPIVSMSDMLRSLSNTKPTVNEQDLEKLKKFTEDF 503          

HSP 2 Score: 77.7962 bits (190), Expect = 1.360e-14
Identity = 36/74 (48.65%), Postives = 51/74 (68.92%), Query Frame = 2
Query:   35 MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFL 256
            M  T +QKAI I  KA+EED+  +F+EA+K Y H V+YFLH IK +   ++    IR KC QYL+R E+L+++L
Sbjct:    1 MEPTNLQKAIAIAQKASEEDQAGHFEEAIKSYHHAVKYFLHIIKREPQGKEGNQKIREKCKQYLDRVEELQDYL 74          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Zebrafish
Match: katnal1 (katanin p60 subunit A-like 1 [Source:NCBI gene;Acc:492790])

HSP 1 Score: 230.72 bits (587), Expect = 2.503e-68
Identity = 146/335 (43.58%), Postives = 195/335 (58.21%), Query Frame = 2
Query:  377 LQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDS-ESAKRIKTEFLVQMQGVSSDNDN------VLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPE-LIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            L   L   IV   PN+HWDD+A L +AK+ L+EAV+LP+  P  F G R PWKG+L+ GPPGTGK+ LAKAVATE   +TFF+VSSS L SK+ GESEKLV+ LF +AR   P+ IFIDEIDS+C  RG +D  E+++R+K+E LVQM GV    ++      V++LAATN PW +D A+RRR EKRIYI LP A  R E+ K+++         +  D   + ++ EGYSGADI+   RDA M  +R        +R +G SP     L  D+L  P                         VT+ D   AL +   +V+  DLEK   + ++F
Sbjct:  188 LVDALERDIVSRNPNIHWDDIADLEDAKKLLREAVVLPMWMPDFFKGIRRPWKGVLMVGPPGTGKTMLAKAVATEC-GTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPTTIFIDEIDSICGRRGTSDEHEASRRVKSELLVQMDGVGGAQESEDPSKMVMVLAATNFPWDIDEALRRRLEKRIYIPLPTAKGRAELLKINLRE---VDVASDVDLTVIAEKIEGYSGADITNVCRDASMMAMR--------RRIQGLSPEEIRALSKDELQMP-------------------------VTMEDFELALKKISKSVSAADLEKYESWMSEF 485          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Xenopus
Match: ENSXETT00000004853.1 (vacuolar protein sorting 4 homolog A [Source:NCBI gene;Acc:100158600])

HSP 1 Score: 582.022 bits (1499), Expect = 0.000e+0
Identity = 296/441 (67.12%), Postives = 361/441 (81.86%), Query Frame = 2
Query:   35 MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            M ++T+QKAI++VTKATEEDKNKN++EAL+LY+H VEYFLH+IKY++HS+K+K+SIRAKC+QYL+RAEKLK++L  +    KK  K    E  +D    G++    +       LQ QL  AIV+EKPNV W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+L+SKWLGESEKLVKNLF LAR+ KPSIIFIDE+DSLC +R +N+SE+A+RIKTEFLVQMQGV ++ND +L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR +MF+L +GN P  H+L +++  EL K+++GYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  NP +IVDDLLTPCSPGDP A+ M W  +  +KL EP V ++DM+++LA  +PTVN  DL K+ +FT DF
Sbjct:    1 MTTSTLQKAIDLVTKATEEDKNKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCMQYLDRAEKLKDYLKTKDKQNKKPVKESQNEKGSDSDSEGENPEKKK-------LQEQLMGAIVMEKPNVRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGNTP--HSLGEENIRELAKKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRTNPGIIVDDLLTPCSPGDPGAVEMTWMEVPSDKLQEPVVCMSDMLRSLATTRPTVNSDDLLKVKKFTEDF 432          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Xenopus
Match: ENSXETT00000005228.1 (vacuolar protein sorting 4 homolog A [Source:NCBI gene;Acc:100158600])

HSP 1 Score: 572.778 bits (1475), Expect = 0.000e+0
Identity = 293/434 (67.51%), Postives = 355/434 (81.80%), Query Frame = 2
Query:   56 KAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            KAI++VTKATEEDKNKN++EAL+LY+H VEYFLH+IKY++HS+K+K+SIRAKC+QYL+RAEKLK++L  +    KK  K    E  +D    G++    +       LQ QL  AIV+EKPNV W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+L+SKWLGESEKLVKNLF LAR+ KPSIIFIDE+DSLC +R +N+SE+A+RIKTEFLVQMQGV ++ND +L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR +MF+L +GN P  H+L +++  EL K+++GYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  NP +IVDDLLTPCSPGDP A+ M W  +  +KL EP V ++DM+++LA  +PTVN  DL K+ +FT DF
Sbjct:    7 KAIDLVTKATEEDKNKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCMQYLDRAEKLKDYLKTKDKQNKKPVKESQNEKGSDSDSEGENPEKKK-------LQEQLMGAIVMEKPNVRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGNTP--HSLGEENIRELAKKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRTNPGIIVDDLLTPCSPGDPGAVEMTWMEVPSDKLQEPVVCMSDMLRSLATTRPTVNSDDLLKVKKFTEDF 431          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Xenopus
Match: ENSXETT00000005102.1 (vacuolar protein sorting 4 homolog A [Source:NCBI gene;Acc:100158600])

HSP 1 Score: 572.392 bits (1474), Expect = 0.000e+0
Identity = 293/434 (67.51%), Postives = 355/434 (81.80%), Query Frame = 2
Query:   56 KAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            KAI++VTKATEEDKNKN++EAL+LY+H VEYFLH+IKY++HS+K+K+SIRAKC+QYL+RAEKLK++L  +    KK  K    E  +D    G++    +       LQ QL  AIV+EKPNV W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+L+SKWLGESEKLVKNLF LAR+ KPSIIFIDE+DSLC +R +N+SE+A+RIKTEFLVQMQGV ++ND +L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR +MF+L +GN P  H+L +++  EL K+++GYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  NP +IVDDLLTPCSPGDP A+ M W  +  +KL EP V ++DM+++LA  +PTVN  DL K+ +FT DF
Sbjct:   11 KAIDLVTKATEEDKNKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCMQYLDRAEKLKDYLKTKDKQNKKPVKESQNEKGSDSDSEGENPEKKK-------LQEQLMGAIVMEKPNVRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGNTP--HSLGEENIRELAKKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRTNPGIIVDDLLTPCSPGDPGAVEMTWMEVPSDKLQEPVVCMSDMLRSLATTRPTVNSDDLLKVKKFTEDF 435          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Xenopus
Match: ENSXETT00000005911.1 (vacuolar protein sorting 4 homolog A [Source:NCBI gene;Acc:100158600])

HSP 1 Score: 570.466 bits (1469), Expect = 0.000e+0
Identity = 291/433 (67.21%), Postives = 354/433 (81.76%), Query Frame = 2
Query:   59 AIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            +I++VTKATEEDKNKN++EAL+LY+H VEYFLH+IKY++HS+K+K+SIRAKC+QYL+RAEKLK++L  +    KK  K    E  +D    G++    +       LQ QL  AIV+EKPNV W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+L+SKWLGESEKLVKNLF LAR+ KPSIIFIDE+DSLC +R +N+SE+A+RIKTEFLVQMQGV ++ND +L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR +MF+L +GN P  H+L +++  EL K+++GYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  NP +IVDDLLTPCSPGDP A+ M W  +  +KL EP V ++DM+++LA  +PTVN  DL K+ +FT DF
Sbjct:   12 SIDLVTKATEEDKNKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCMQYLDRAEKLKDYLKTKDKQNKKPVKESQNEKGSDSDSEGENPEKKK-------LQEQLMGAIVMEKPNVRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGNTP--HSLGEENIRELAKKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRTNPGIIVDDLLTPCSPGDPGAVEMTWMEVPSDKLQEPVVCMSDMLRSLATTRPTVNSDDLLKVKKFTEDF 435          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Xenopus
Match: ENSXETT00000005821.1 (vacuolar protein sorting 4 homolog A [Source:NCBI gene;Acc:100158600])

HSP 1 Score: 570.466 bits (1469), Expect = 0.000e+0
Identity = 292/433 (67.44%), Postives = 354/433 (81.76%), Query Frame = 2
Query:   59 AIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            AI++VTKATEEDKNKN++EAL+LY+H VEYFLH+IKY++HS+K+K+SIRAKC+QYL+RAEKLK++L  +    KK  K    E  +D    G++    +       LQ QL  AIV+EKPNV W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+L+SKWLGESEKLVKNLF LAR+ KPSIIFIDE+DSLC +R +N+SE+A+RIKTEFLVQMQGV ++ND +L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR +MF+L +GN P  H+L +++  EL K+++GYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  NP +IVDDLLTPCSPGDP A+ M W  +  +KL EP V ++DM+++LA  +PTVN  DL K+ +FT DF
Sbjct:    3 AIDLVTKATEEDKNKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCMQYLDRAEKLKDYLKTKDKQNKKPVKESQNEKGSDSDSEGENPEKKK-------LQEQLMGAIVMEKPNVRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGNTP--HSLGEENIRELAKKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRTNPGIIVDDLLTPCSPGDPGAVEMTWMEVPSDKLQEPVVCMSDMLRSLATTRPTVNSDDLLKVKKFTEDF 426          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Mouse
Match: Vps4a (vacuolar protein sorting 4A [Source:MGI Symbol;Acc:MGI:1890520])

HSP 1 Score: 574.318 bits (1479), Expect = 0.000e+0
Identity = 296/441 (67.12%), Postives = 361/441 (81.86%), Query Frame = 2
Query:   35 MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            M ++T+QKAI++VTKATEEDK KN++EAL+LY+H VEYFLH+IKY++HS+K+K+SIRAKC+QYL+RAEKLK++L      K+KH K    E  N  +  G        + EKKKLQ QL  A+V+EKPN+ W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+L+SKWLGESEKLVKNLF LAR+ KPSIIFIDE+DSLC +R +N+SE+A+RIKTEFLVQMQGV ++ND  L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR +MF+L +G+ P  H LT  +  EL +++EGYSGADISI VRD+LM PVRKVQ+ATHFK+  GPS  NP +++DDLLTPCSPGDP AI M W  + G+KLLEP V ++DM+++LA  +PTVN  DL K+ +F+ DF
Sbjct:    1 MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCMQYLDRAEKLKDYLR----NKEKHGKKPVKE--NQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVMEKPNIRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGSTP--HNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQSATHFKKVCGPSRTNPSVMIDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNADDLLKVKKFSEDF 433          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Mouse
Match: Vps4b (vacuolar protein sorting 4B [Source:MGI Symbol;Acc:MGI:1100499])

HSP 1 Score: 545.814 bits (1405), Expect = 0.000e+0
Identity = 299/443 (67.49%), Postives = 359/443 (81.04%), Query Frame = 2
Query:   35 MASTT--IQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            MAST   +QKAI++ +KA +EDK  N++EAL+LY+H V+YFLH +KY++  +K+K SIRAKC +YL+RAEKLKE+L  +    +K  K     G  D+K N         D EKKKLQ+QL  AIV+E+PNV W DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFS+SSS+LVSKWLGESEKLVKNLF LARE KPSIIFIDEIDSLC +R +N+SE+A+RIKTEFLVQMQGV  DND +L+L ATNIPW LDSAIRRRFEKRIYI LPEA AR  MF+L +G+   Q++LT+ DF ELG++++GYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  +P  IV+DLLTPCSPGDP AI M W  + G+KLLEP V++ DM+++L+  KPTVN++DL KL +FT DF
Sbjct:    1 MASTNTNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKKKEKKPQKPVKE-EQSGPVDEKGNDSDGEAESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEAHARAAMFRLHLGST--QNSLTEADFQELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNCIVNDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMWDMLRSLSSTKPTVNEQDLLKLKKFTEDF 440          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Mouse
Match: Vps4b (vacuolar protein sorting 4B [Source:MGI Symbol;Acc:MGI:1100499])

HSP 1 Score: 545.814 bits (1405), Expect = 0.000e+0
Identity = 299/443 (67.49%), Postives = 359/443 (81.04%), Query Frame = 2
Query:   35 MASTT--IQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            MAST   +QKAI++ +KA +EDK  N++EAL+LY+H V+YFLH +KY++  +K+K SIRAKC +YL+RAEKLKE+L  +    +K  K     G  D+K N         D EKKKLQ+QL  AIV+E+PNV W DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFS+SSS+LVSKWLGESEKLVKNLF LARE KPSIIFIDEIDSLC +R +N+SE+A+RIKTEFLVQMQGV  DND +L+L ATNIPW LDSAIRRRFEKRIYI LPEA AR  MF+L +G+   Q++LT+ DF ELG++++GYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  +P  IV+DLLTPCSPGDP AI M W  + G+KLLEP V++ DM+++L+  KPTVN++DL KL +FT DF
Sbjct:    1 MASTNTNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKKKEKKPQKPVKE-EQSGPVDEKGNDSDGEAESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEAHARAAMFRLHLGST--QNSLTEADFQELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNCIVNDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMWDMLRSLSSTKPTVNEQDLLKLKKFTEDF 440          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Mouse
Match: Katnal1 (katanin p60 subunit A-like 1 [Source:MGI Symbol;Acc:MGI:2387638])

HSP 1 Score: 227.639 bits (579), Expect = 3.626e-67
Identity = 138/286 (48.25%), Postives = 182/286 (63.64%), Query Frame = 2
Query:  377 LQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDS-ESAKRIKTEFLVQMQGVSS--DNDN----VLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGN---KPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPE-LIVDDLLTPCSPGD 1201
            L   L   IV   P++HWDD+A L  AK+ L+EAV+LP+  P  F G R PWKG+L+ GPPGTGK+ LAKAVATE   +TFF+VSSS L SK+ GESEKLV+ LF +AR   P+ IFIDEIDS+CS RG +D  E+++R+K+E L+QM GV    +ND+    V++LAATN PW +D A+RRR EKRIYI LP A  R E+ K+S+      P  H        ++  ++EGYSGADI+   RDA +  +R        +R  G SP     L  ++L  P + GD
Sbjct:  188 LVEALERDIVSRNPSIHWDDIADLEEAKKLLREAVVLPMWMPDFFKGIRRPWKGVLMVGPPGTGKTMLAKAVATEC-GTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPTTIFIDEIDSICSRRGTSDEHEASRRVKSELLIQMDGVGGALENDDPSKMVMVLAATNFPWDIDEALRRRLEKRIYIPLPTAKGRAELLKISLREVELDPDVH------LEDIADKTEGYSGADITNICRDASLMAMR--------RRINGLSPEEIRALSKEELQMPVTRGD 458          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Mouse
Match: Katnal2 (katanin p60 subunit A-like 2 [Source:MGI Symbol;Acc:MGI:1924234])

HSP 1 Score: 226.868 bits (577), Expect = 7.934e-67
Identity = 123/241 (51.04%), Postives = 165/241 (68.46%), Query Frame = 2
Query:  401 IVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRG---DNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTL---TQQDFVELGKRSEGYSGADISIAVRDALMFPVRKV 1105
            I +  PN+ W+D+ GL  AK+ +KEAV+ PI++P LFTG  +PWKG+LLYGPPGTGK+ LAKAVATE   +TFF++S+S +VSKW G+SEKLV+ LF LAR   PS IF+DE++S+ S RG     + E + R+KTE LVQM G++   D V +LAA+N+PW LD A+ RR EKRI + LP   AR  M    +      H L   TQ ++  L + +EGYSG+DI +  R+A M PVRK+
Sbjct:  201 IYLHNPNIKWNDIIGLDAAKQLVKEAVVYPIRYPQLFTGILSPWKGLLLYGPPGTGKTLLAKAVATEC-KTTFFNISASTIVSKWRGDSEKLVRVLFELARYHAPSTIFLDELESVMSQRGMVPGGEHEGSLRMKTELLVQMDGLARSEDLVFVLAASNLPWELDCAMLRRLEKRILVDLPSQEARQAMIYHWLPPVSKNHALELHTQLEYSVLSQETEGYSGSDIKLVCREAAMRPVRKI 440          
BLAST of Vacuolar protein sorting 4 vs. UniProt/SwissProt
Match: sp|Q9UN37|VPS4A_HUMAN (Vacuolar protein sorting-associated protein 4A OS=Homo sapiens OX=9606 GN=VPS4A PE=1 SV=1)

HSP 1 Score: 575.474 bits (1482), Expect = 0.000e+0
Identity = 295/441 (66.89%), Postives = 362/441 (82.09%), Query Frame = 2
Query:   35 MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            M ++T+QKAI++VTKATEEDK KN++EAL+LY+H VEYFLH+IKY++HS+K+K+SIRAKC+QYL+RAEKLK++L +    K+KH K    E  N  +  G        + EKKKLQ QL  A+V+EKPN+ W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+L+SKWLGESEKLVKNLF LAR+ KPSIIFIDE+DSLC +R +N+SE+A+RIKTEFLVQMQGV ++ND  L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR +MF+L +G+ P  H LT  +  EL +++EGYSGADISI VRD+LM PVRKVQ+ATHFK+  GPS  NP +++DDLLTPCSPGDP A+ M W  + G+KLLEP V ++DM+++LA  +PTVN  DL K+ +F+ DF
Sbjct:    1 MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCVQYLDRAEKLKDYLRS----KEKHGKKPVKE--NQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVMEKPNIRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGSTP--HNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQSATHFKKVCGPSRTNPSMMIDDLLTPCSPGDPGAMEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNADDLLKVKKFSEDF 433          
BLAST of Vacuolar protein sorting 4 vs. UniProt/SwissProt
Match: sp|Q793F9|VPS4A_RAT (Vacuolar protein sorting-associated protein 4A OS=Rattus norvegicus OX=10116 GN=Vps4a PE=1 SV=1)

HSP 1 Score: 574.318 bits (1479), Expect = 0.000e+0
Identity = 296/441 (67.12%), Postives = 361/441 (81.86%), Query Frame = 2
Query:   35 MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            M ++T+QKAI++VTKATEEDK KN++EAL+LY+H VEYFLH+IKY++HS+K+K+SIRAKC+QYL+RAEKLK++L      K+KH K    E  N  +  G        + EKKKLQ QL  A+V+EKPN+ W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+L+SKWLGESEKLVKNLF LAR+ KPSIIFIDE+DSLC +R +N+SE+A+RIKTEFLVQMQGV ++ND  L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR +MF+L +G+ P  H LT  +  EL +++EGYSGADISI VRD+LM PVRKVQ+ATHFK+  GPS  NP +++DDLLTPCSPGDP AI M W  + G+KLLEP V ++DM+++LA  +PTVN  DL K+ +F+ DF
Sbjct:    1 MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCMQYLDRAEKLKDYLR----NKEKHGKKPVKE--NQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVMEKPNIRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGSTP--HNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQSATHFKKVCGPSRTNPSVMIDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNADDLLKVKKFSEDF 433          
BLAST of Vacuolar protein sorting 4 vs. UniProt/SwissProt
Match: sp|Q8VEJ9|VPS4A_MOUSE (Vacuolar protein sorting-associated protein 4A OS=Mus musculus OX=10090 GN=Vps4a PE=1 SV=1)

HSP 1 Score: 574.318 bits (1479), Expect = 0.000e+0
Identity = 296/441 (67.12%), Postives = 361/441 (81.86%), Query Frame = 2
Query:   35 MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            M ++T+QKAI++VTKATEEDK KN++EAL+LY+H VEYFLH+IKY++HS+K+K+SIRAKC+QYL+RAEKLK++L      K+KH K    E  N  +  G        + EKKKLQ QL  A+V+EKPN+ W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+L+SKWLGESEKLVKNLF LAR+ KPSIIFIDE+DSLC +R +N+SE+A+RIKTEFLVQMQGV ++ND  L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR +MF+L +G+ P  H LT  +  EL +++EGYSGADISI VRD+LM PVRKVQ+ATHFK+  GPS  NP +++DDLLTPCSPGDP AI M W  + G+KLLEP V ++DM+++LA  +PTVN  DL K+ +F+ DF
Sbjct:    1 MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCMQYLDRAEKLKDYLR----NKEKHGKKPVKE--NQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVMEKPNIRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARAQMFRLHLGSTP--HNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQSATHFKKVCGPSRTNPSVMIDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNADDLLKVKKFSEDF 433          
BLAST of Vacuolar protein sorting 4 vs. UniProt/SwissProt
Match: sp|Q0VD48|VPS4B_BOVIN (Vacuolar protein sorting-associated protein 4B OS=Bos taurus OX=9913 GN=VPS4B PE=2 SV=1)

HSP 1 Score: 563.918 bits (1452), Expect = 0.000e+0
Identity = 306/445 (68.76%), Postives = 364/445 (81.80%), Query Frame = 2
Query:   35 MAST--TIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLA-GEGANXXXXXXXXXXXXXXXX-XXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            MAST   +QKAI++ +KA +EDK  N++EAL+LY+H V+YFLH +KY++  +K+K SIRAKC +YL+RAEKLKE+L      +KK +K +  G+ A   +K  DSD + E D  EKKKLQ+QL  AIV+E+PNV W DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFS+SSS+LVSKWLGESEKLVKNLF LARE KPSIIFIDEIDSLC +R +N+SE+A+RIKTEFLVQMQGV  DND +L+L ATNIPW LDSAIRRRFEKRIYI LPEA AR  MFKL +G    Q++LT+ DF +LGK++EGYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  +P  IVDDLLTPCSPGDP AI M W  + G+KLLEP V ++DM+++L+  KPTVN+ DL KL +FT DF
Sbjct:    1 MASTPTNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKKR---EKKPQKPVKEGQPAPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEAHARAAMFKLHLGTT--QNSLTEADFRDLGKKTEGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNNIVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVCMSDMLRSLSSTKPTVNEHDLLKLKKFTEDF 440          
BLAST of Vacuolar protein sorting 4 vs. UniProt/SwissProt
Match: sp|O75351|VPS4B_HUMAN (Vacuolar protein sorting-associated protein 4B OS=Homo sapiens OX=9606 GN=VPS4B PE=1 SV=2)

HSP 1 Score: 561.222 bits (1445), Expect = 0.000e+0
Identity = 298/447 (66.67%), Postives = 359/447 (80.31%), Query Frame = 2
Query:   35 MASTT--IQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEK----SLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            M+ST+  +QKAI++ +KA +EDK  N++EAL+LY+H V+YFLH +KY++  +K+K SIRAKC +YL+RAEKLKE+L  +    +K  K    S A E  ND    G+SD   +K      LQ+QL  AIV+E+PNV W DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFS+SSS+LVSKWLGESEKLVKNLF LARE KPSIIFIDEIDSLC +R +N+SE+A+RIKTEFLVQMQGV  DND +L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR  MFKL +G    Q++LT+ DF ELG++++GYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  +P  +VDDLLTPCSPGDP AI M W  + G+KLLEP V+++DM+++L+  KPTVN+ DL KL +FT DF
Sbjct:    1 MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKK-----LQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTT--QNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDF 440          
BLAST of Vacuolar protein sorting 4 vs. TrEMBL
Match: A0A1S3J3G6 (vacuolar protein sorting-associated protein 4A isoform X2 OS=Lingula unguis OX=7574 GN=LOC106169754 PE=3 SV=1)

HSP 1 Score: 611.683 bits (1576), Expect = 0.000e+0
Identity = 323/439 (73.58%), Postives = 377/439 (85.88%), Query Frame = 2
Query:   41 STTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            ST++QKAI+IVTKATEEDK KN++EAL+LYEHGVEYFLH+IKY++HS+K+K+SIRAKC+ YL+RAEKLK+ +S   G K+K  K  +  G   K  N  SDSDSE + EKKK Q+QL  AIV+EKPN+ WDDVAGL  AKEALKEAVILPIKFPHLFTGKR PW+GILLYGPPGTGKSYLAKAVATEANNSTFFSVSSS+LVSKWLGESEKLVKNLF +ARE KPSIIFIDE+DSLCS+R DN+SESA+RIKTEFLVQMQGV +DN+ +L+L ATNIPW LDSAIRRRFEKRIYI LPEAPAR  MF+L +GN P  ++LTQ+DF ELG R+EGYSGADISI VRDALM PVRKVQTATHFKR RGPS  NP++IV+DLLTPCSPGDP A+ MNW  + G+KL EP V+++D + +L R+KPTVN++DL+KL  FTNDF
Sbjct:    4 STSLQKAIDIVTKATEEDKKKNYEEALRLYEHGVEYFLHAIKYEAHSDKAKESIRAKCVSYLDRAEKLKQHVS---GKKQKPVKDGSSGGNKKKGDNDSSDSDSEGNAEKKKFQTQLEGAIVMEKPNIKWDDVAGLDGAKEALKEAVILPIKFPHLFTGKRQPWRGILLYGPPGTGKSYLAKAVATEANNSTFFSVSSSDLVSKWLGESEKLVKNLFEMAREHKPSIIFIDEVDSLCSSRSDNESESARRIKTEFLVQMQGVGNDNEGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEAPARATMFQLHLGNTP--NSLTQEDFRELGLRTEGYSGADISIVVRDALMQPVRKVQTATHFKRVRGPSRDNPDMIVNDLLTPCSPGDPQAMEMNWVDVPGDKLFEPVVSMSDALNSLTRSKPTVNEEDLKKLEAFTNDF 437          
BLAST of Vacuolar protein sorting 4 vs. TrEMBL
Match: A0A1S3J323 (vacuolar protein sorting-associated protein 4A isoform X1 OS=Lingula unguis OX=7574 GN=LOC106169754 PE=3 SV=1)

HSP 1 Score: 608.216 bits (1567), Expect = 0.000e+0
Identity = 321/439 (73.12%), Postives = 376/439 (85.65%), Query Frame = 2
Query:   41 STTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            ST++QKAI+IVTKATEEDK KN++EAL+LYEHGVEYFLH+IKY++HS+K+K+SIRAKC+ YL+RAEKLK+ +S   G K+K  K  +  G   K  N  SDSDSE + EKKK Q+QL  AIV+EKPN+ WDDVAGL  AKEALKEAVILPIKFPHLFTGKR PW+GILLYGPPGTGKSYLAKAVATEANNSTFFSVSSS+LVSKWLGESEKLVKNLF +ARE KPSIIFIDE+DSLCS+R DN+SESA+RIKTEFLVQMQGV +DN+ +L+L ATNIPW LDSAIRRRFEKRIYI LPEAPAR  MF+L +GN P  ++LTQ+DF ELG R+EGYSGADISI VRDALM PVRKVQTATHFKR RGPS  NP++IV+DLLTPCSPGDP A+ MNW  + G+KL EP V+++DM+ +L   KPTVN+ DL+K+ +FT+DF
Sbjct:    4 STSLQKAIDIVTKATEEDKKKNYEEALRLYEHGVEYFLHAIKYEAHSDKAKESIRAKCVSYLDRAEKLKQHVS---GKKQKPVKDGSSGGNKKKGDNDSSDSDSEGNAEKKKFQTQLEGAIVMEKPNIKWDDVAGLDGAKEALKEAVILPIKFPHLFTGKRQPWRGILLYGPPGTGKSYLAKAVATEANNSTFFSVSSSDLVSKWLGESEKLVKNLFEMAREHKPSIIFIDEVDSLCSSRSDNESESARRIKTEFLVQMQGVGNDNEGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEAPARATMFQLHLGNTP--NSLTQEDFRELGLRTEGYSGADISIVVRDALMQPVRKVQTATHFKRVRGPSRDNPDMIVNDLLTPCSPGDPQAMEMNWVDVPGDKLFEPVVSMSDMLMSLGVMKPTVNKADLDKIQKFTDDF 437          
BLAST of Vacuolar protein sorting 4 vs. TrEMBL
Match: A0A1S3J3I0 (vacuolar protein sorting-associated protein 4A isoform X2 OS=Lingula unguis OX=7574 GN=LOC106169755 PE=3 SV=1)

HSP 1 Score: 607.446 bits (1565), Expect = 0.000e+0
Identity = 320/439 (72.89%), Postives = 376/439 (85.65%), Query Frame = 2
Query:   41 STTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            ST++QKAI+IVTKATEEDK KN++EAL+LYEHGVEYFLH+IKY++HS+K+K+SIRAKC+ YL+RAEKLK+ +S  G  +K  +   +G   N K  N  SDSDSE + EKKK Q+QL  AIV+EKPN+ WDDVAGL  AKEALKEAVILPIKFPHLFTGKR PW+GILLYGPPGTGKSYLAKAVATEANNSTFFSVSSS+LVSKWLGESEKLVKNLF +ARE KPSIIFIDE+DSLCS+R DN+SESA+RIKTEFLVQMQGV +DN+ +L+L ATNIPW LDSAIRRRFEKRIYI LPEAPAR  MF+L +GN P  ++LTQ+DF ELG R+EGYSGADISI VRDALM PVRKVQTATHFKR RGPS  NP++IV+DLLTPCSPGDP A+ MNW  + G+KL EP V+++DM+ +L   KPTVN+ DL+K+ +FT+DF
Sbjct:    4 STSLQKAIDIVTKATEEDKKKNYEEALRLYEHGVEYFLHAIKYEAHSDKAKESIRAKCVSYLDRAEKLKQHVS--GKKQKPVKDGSSGGNKNRKGDNDSSDSDSEGNAEKKKFQTQLEGAIVMEKPNIKWDDVAGLDGAKEALKEAVILPIKFPHLFTGKRQPWRGILLYGPPGTGKSYLAKAVATEANNSTFFSVSSSDLVSKWLGESEKLVKNLFEMAREHKPSIIFIDEVDSLCSSRSDNESESARRIKTEFLVQMQGVGNDNEGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEAPARATMFQLHLGNTP--NSLTQEDFRELGLRTEGYSGADISIVVRDALMQPVRKVQTATHFKRVRGPSRDNPDMIVNDLLTPCSPGDPQAMEMNWVDVPGDKLFEPVVSMSDMLMSLGVMKPTVNKADLDKIQKFTDDF 438          
BLAST of Vacuolar protein sorting 4 vs. TrEMBL
Match: A0A1S3J4P7 (vacuolar protein sorting-associated protein 4A isoform X3 OS=Lingula unguis OX=7574 GN=LOC106169755 PE=3 SV=1)

HSP 1 Score: 606.29 bits (1562), Expect = 0.000e+0
Identity = 320/439 (72.89%), Postives = 376/439 (85.65%), Query Frame = 2
Query:   41 STTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            +T++QKAI+IVTKATEEDK KN++EAL+LYEHGVEYFLH+IKY++HS+K+K+SIRAKC+ YL+RAEKLK+ +S   G K+K  K  +  G   K  N  SDSDSE + EKKK Q+QL  AIV+EKPN+ WDDVAGL  AKEALKEAVILPIKFPHLFTGKR PW+GILLYGPPGTGKSYLAKAVATEANNSTFFSVSSS+LVSKWLGESEKLVKNLF +ARE KPSIIFIDE+DSLCS+R DN+SESA+RIKTEFLVQMQGV +DN+ +L+L ATNIPW LDSAIRRRFEKRIYI LPEAPAR  MF+L +GN P  ++LTQ+DF ELG R+EGYSGADISI VRDALM PVRKVQTATHFKR RGPS  NP++IV+DLLTPCSPGDP A+ MNW  + G+KL EP V+++DM+ +L   KPTVN+ DL+K+ +FT+DF
Sbjct:    4 ATSLQKAIDIVTKATEEDKKKNYEEALRLYEHGVEYFLHAIKYEAHSDKAKESIRAKCVSYLDRAEKLKQHVS---GKKQKPVKDGSSGGNKKKGDNDSSDSDSEGNAEKKKFQTQLEGAIVMEKPNIKWDDVAGLDGAKEALKEAVILPIKFPHLFTGKRQPWRGILLYGPPGTGKSYLAKAVATEANNSTFFSVSSSDLVSKWLGESEKLVKNLFEMAREHKPSIIFIDEVDSLCSSRSDNESESARRIKTEFLVQMQGVGNDNEGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEAPARATMFQLHLGNTP--NSLTQEDFRELGLRTEGYSGADISIVVRDALMQPVRKVQTATHFKRVRGPSRDNPDMIVNDLLTPCSPGDPQAMEMNWVDVPGDKLFEPVVSMSDMLMSLGVMKPTVNKADLDKIQKFTDDF 437          
BLAST of Vacuolar protein sorting 4 vs. TrEMBL
Match: A0A1S3J318 (vacuolar protein sorting-associated protein 4A isoform X1 OS=Lingula unguis OX=7574 GN=LOC106169755 PE=3 SV=1)

HSP 1 Score: 605.905 bits (1561), Expect = 0.000e+0
Identity = 319/439 (72.67%), Postives = 376/439 (85.65%), Query Frame = 2
Query:   41 STTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            +T++QKAI+IVTKATEEDK KN++EAL+LYEHGVEYFLH+IKY++HS+K+K+SIRAKC+ YL+RAEKLK+ +S  G  +K  +   +G   N K  N  SDSDSE + EKKK Q+QL  AIV+EKPN+ WDDVAGL  AKEALKEAVILPIKFPHLFTGKR PW+GILLYGPPGTGKSYLAKAVATEANNSTFFSVSSS+LVSKWLGESEKLVKNLF +ARE KPSIIFIDE+DSLCS+R DN+SESA+RIKTEFLVQMQGV +DN+ +L+L ATNIPW LDSAIRRRFEKRIYI LPEAPAR  MF+L +GN P  ++LTQ+DF ELG R+EGYSGADISI VRDALM PVRKVQTATHFKR RGPS  NP++IV+DLLTPCSPGDP A+ MNW  + G+KL EP V+++DM+ +L   KPTVN+ DL+K+ +FT+DF
Sbjct:    4 ATSLQKAIDIVTKATEEDKKKNYEEALRLYEHGVEYFLHAIKYEAHSDKAKESIRAKCVSYLDRAEKLKQHVS--GKKQKPVKDGSSGGNKNRKGDNDSSDSDSEGNAEKKKFQTQLEGAIVMEKPNIKWDDVAGLDGAKEALKEAVILPIKFPHLFTGKRQPWRGILLYGPPGTGKSYLAKAVATEANNSTFFSVSSSDLVSKWLGESEKLVKNLFEMAREHKPSIIFIDEVDSLCSSRSDNESESARRIKTEFLVQMQGVGNDNEGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEAPARATMFQLHLGNTP--NSLTQEDFRELGLRTEGYSGADISIVVRDALMQPVRKVQTATHFKRVRGPSRDNPDMIVNDLLTPCSPGDPQAMEMNWVDVPGDKLFEPVVSMSDMLMSLGVMKPTVNKADLDKIQKFTDDF 438          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Cavefish
Match: vps4a (vacuolar protein sorting 4 homolog A [Source:NCBI gene;Acc:103042556])

HSP 1 Score: 579.711 bits (1493), Expect = 0.000e+0
Identity = 292/442 (66.06%), Postives = 363/442 (82.13%), Query Frame = 2
Query:   35 MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFL-SAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            M ++T+QKAI++VTKATEEDK KN++EAL+LY+H VEYFLH+IKY++HS+K+K+SIR KC+QYL+RAEKLKE+L + +  GKK  +++ + + ++   +  + +           LQ  L  AIV+EKPNV W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+L+SKWLGESEKLV+NLF LAR +KPSIIFIDE+DSLC +R +N+SE+A+RIKTEFLVQMQGV ++ND +L+L ATNIPW LD+AIRRRFEKRIYI LPE PAR  MF+L +GN P  H+LT+ D  +L ++++GYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  NP + VDDLLTPCSPGDP AI M W  + G+KLLEP V + DM+++LA  +PTVN +DL K+ +FT DF
Sbjct:    1 MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRGKCMQYLDRAEKLKEYLKNKDKQGKKPVKEAQSNDKSDSDSEGENPEKKK--------LQEHLMGAIVMEKPNVSWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVRNLFELARLRKPSIIFIDEVDSLCGSRSENESEAARRIKTEFLVQMQGVGNNNDKILVLGATNIPWVLDAAIRRRFEKRIYIPLPEEPARAAMFRLHLGNTP--HSLTEADLRQLARKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRSNPSMTVDDLLTPCSPGDPAAIEMTWMDVPGDKLLEPIVCMADMLRSLATTRPTVNAEDLLKVKKFTEDF 432          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Cavefish
Match: vps4a (vacuolar protein sorting 4 homolog A [Source:NCBI gene;Acc:103042556])

HSP 1 Score: 573.548 bits (1477), Expect = 0.000e+0
Identity = 289/437 (66.13%), Postives = 359/437 (82.15%), Query Frame = 2
Query:   50 IQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFL-SAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            ++KAI++VTKATEEDK KN++EAL+LY+H VEYFLH+IKY++HS+K+K+SIR KC+QYL+RAEKLKE+L + +  GKK  +++ + + ++   +  + +           LQ  L  AIV+EKPNV W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+L+SKWLGESEKLV+NLF LAR +KPSIIFIDE+DSLC +R +N+SE+A+RIKTEFLVQMQGV ++ND +L+L ATNIPW LD+AIRRRFEKRIYI LPE PAR  MF+L +GN P  H+LT+ D  +L ++++GYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  NP + VDDLLTPCSPGDP AI M W  + G+KLLEP V + DM+++LA  +PTVN +DL K+ +FT DF
Sbjct:   30 VEKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRGKCMQYLDRAEKLKEYLKNKDKQGKKPVKEAQSNDKSDSDSEGENPEKKK--------LQEHLMGAIVMEKPNVSWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVRNLFELARLRKPSIIFIDEVDSLCGSRSENESEAARRIKTEFLVQMQGVGNNNDKILVLGATNIPWVLDAAIRRRFEKRIYIPLPEEPARAAMFRLHLGNTP--HSLTEADLRQLARKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRSNPSMTVDDLLTPCSPGDPAAIEMTWMDVPGDKLLEPIVCMADMLRSLATTRPTVNAEDLLKVKKFTEDF 456          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Cavefish
Match: vps4a (vacuolar protein sorting 4 homolog A [Source:NCBI gene;Acc:103042556])

HSP 1 Score: 570.466 bits (1469), Expect = 0.000e+0
Identity = 288/434 (66.36%), Postives = 357/434 (82.26%), Query Frame = 2
Query:   35 MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFL-SAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEK 1333
            M ++T+QKAI++VTKATEEDK KN++EAL+LY+H VEYFLH+IKY++HS+K+K+SIR KC+QYL+RAEKLKE+L + +  GKK  +++ + + ++   +  + +           LQ  L  AIV+EKPNV W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+L+SKWLGESEKLV+NLF LAR +KPSIIFIDE+DSLC +R +N+SE+A+RIKTEFLVQMQGV ++ND +L+L ATNIPW LD+AIRRRFEKRIYI LPE PAR  MF+L +GN P  H+LT+ D  +L ++++GYSGADISI VRDALM PVRKVQ+ATHFK+ RGPS  NP + VDDLLTPCSPGDP AI M W  + G+KLLEP V + DM+++LA  +PTVN +DL K
Sbjct:    1 MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRGKCMQYLDRAEKLKEYLKNKDKQGKKPVKEAQSNDKSDSDSEGENPEKKK--------LQEHLMGAIVMEKPNVSWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVRNLFELARLRKPSIIFIDEVDSLCGSRSENESEAARRIKTEFLVQMQGVGNNNDKILVLGATNIPWVLDAAIRRRFEKRIYIPLPEEPARAAMFRLHLGNTP--HSLTEADLRQLARKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRSNPSMTVDDLLTPCSPGDPAAIEMTWMDVPGDKLLEPIVCMADMLRSLATTRPTVNAEDLLK 424          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Cavefish
Match: ENSAMXT00000018479.2 (vacuolar protein sorting-associated protein 4B-like [Source:NCBI gene;Acc:103033151])

HSP 1 Score: 546.969 bits (1408), Expect = 0.000e+0
Identity = 285/436 (65.37%), Postives = 349/436 (80.05%), Query Frame = 2
Query:   50 IQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            +QKAI++ T+A EEDK KN++EAL+LY+H V+YFLH +KY++  +K+K SIRAKC  YL+RAE+LKE+L  +          ++ +  N+     +S+           LQ QL  AIV+EKPNV W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFS+SSS+LVSKWLGESE+LVKNLF +AR+ KPSIIFIDEIDSLC +R DN+SE+A+RIKTEFLVQMQGV +DN+ +L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR+ MFKL++G  P   +LT +DF  LGK++EGYSGADISI VRDALM P+RKVQ+ATHFK+ RG +  NP+ +VDDLLTPCSPGDP+AI M W  + G+KLLEP V + DM ++L + KPTVN++DLEKL +FT DF
Sbjct:    4 LQKAIDLATRAAEEDKAKNYEEALRLYQHSVQYFLHVVKYEAQGDKAKQSIRAKCADYLDRAEQLKEYLKKKEKAPPSKPVKVSDDKGNESDDGENSEKKK--------LQGQLQGAIVMEKPNVKWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESERLVKNLFSMARQHKPSIIFIDEIDSLCGSRSDNESEAARRIKTEFLVQMQGVGNDNEGILVLGATNIPWTLDSAIRRRFEKRIYIPLPEEHARSFMFKLNLGTTPS--SLTDEDFHTLGKKTEGYSGADISIIVRDALMQPIRKVQSATHFKKVRGKTWNNPDAVVDDLLTPCSPGDPNAIEMTWVDVPGDKLLEPIVCMADMQRSLDKTKPTVNEEDLEKLKKFTEDF 429          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Cavefish
Match: ENSAMXT00000044404.1 (vacuolar protein sorting-associated protein 4B-like [Source:NCBI gene;Acc:103033151])

HSP 1 Score: 546.199 bits (1406), Expect = 0.000e+0
Identity = 285/439 (64.92%), Postives = 350/439 (79.73%), Query Frame = 2
Query:   41 STTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            S ++ KAI++ T+A EEDK KN++EAL+LY+H V+YFLH +KY++  +K+K SIRAKC  YL+RAE+LKE+L  +          ++ +  N+     +S+           LQ QL  AIV+EKPNV W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFS+SSS+LVSKWLGESE+LVKNLF +AR+ KPSIIFIDEIDSLC +R DN+SE+A+RIKTEFLVQMQGV +DN+ +L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR+ MFKL++G  P   +LT +DF  LGK++EGYSGADISI VRDALM P+RKVQ+ATHFK+ RG +  NP+ +VDDLLTPCSPGDP+AI M W  + G+KLLEP V + DM ++L + KPTVN++DLEKL +FT DF
Sbjct:    7 SFSLPKAIDLATRAAEEDKAKNYEEALRLYQHSVQYFLHVVKYEAQGDKAKQSIRAKCADYLDRAEQLKEYLKKKEKAPPSKPVKVSDDKGNESDDGENSEKKK--------LQGQLQGAIVMEKPNVKWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESERLVKNLFSMARQHKPSIIFIDEIDSLCGSRSDNESEAARRIKTEFLVQMQGVGNDNEGILVLGATNIPWTLDSAIRRRFEKRIYIPLPEEHARSFMFKLNLGTTPS--SLTDEDFHTLGKKTEGYSGADISIIVRDALMQPIRKVQSATHFKKVRGKTWNNPDAVVDDLLTPCSPGDPNAIEMTWVDVPGDKLLEPIVCMADMQRSLDKTKPTVNEEDLEKLKKFTEDF 435          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003236.1 (pep scaffold:Pmarinus_7.0:GL477724:37443:44438:-1 gene:ENSPMAG00000002921.1 transcript:ENSPMAT00000003236.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 588.186 bits (1515), Expect = 0.000e+0
Identity = 303/437 (69.34%), Postives = 363/437 (83.07%), Query Frame = 2
Query:   50 IQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFL-SAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            +QKAI++VTKATEEDK KN++EAL+LY+H VEYFLH+IKY++ SEK+K+SIRAKC QYLERAEKLKE+L + E GGKK  ++S + +  +D    G++    +       +Q QL  AIV+EKPNV W DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+LVSKWLGESEKLVKNLF +AR+ KPSIIFIDE+DSLC +R +N+SE+A+RIKTEFLVQMQGV  DND +L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR  MFKL +GN P   +L++ DF +LGK++EGYSGADISI VRDALM PVRKVQ+ATHFKR RGPS  +P + VDDLLTPCSPGDP+AI M W  + G+KLLEP V+ +DM+++LA  +PTVN +DL+KL +FT+DF
Sbjct:    9 LQKAIDLVTKATEEDKAKNYEEALRLYQHSVEYFLHAIKYEAQSEKAKESIRAKCKQYLERAEKLKEYLRTKEKGGKKPVKESQSNDKGSDSDSEGENPEKKK-------MQEQLQSAIVMEKPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLVSKWLGESEKLVKNLFEMARQHKPSIIFIDEVDSLCGSRNENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWTLDSAIRRRFEKRIYIPLPEEQARLSMFKLHLGNTP--LSLSETDFRDLGKKTEGYSGADISIVVRDALMQPVRKVQSATHFKRVRGPSRDDPNMFVDDLLTPCSPGDPNAIEMTWMDVPGDKLLEPVVSTSDMLRSLATTRPTVNSEDLKKLQKFTDDF 436          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Sea Lamprey
Match: katnal2 (katanin p60 subunit A-like 2 [Source:ZFIN;Acc:ZDB-GENE-051113-156])

HSP 1 Score: 226.868 bits (577), Expect = 4.549e-67
Identity = 133/276 (48.19%), Postives = 181/276 (65.58%), Query Frame = 2
Query:  377 LQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNR----GDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSM---GNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLT 1183
            L S ++  I ++ PNV WDD+ GL  AK  +KEAV+ PIK+P LFTG  +PWKG+LLYGPPGTGK+ LAKAVATE   +TFF++S+S++VSKW G+SEKLVK LF LAR   PS IF+DE++S+   R       + E ++R+KTE LVQM G++  +D V +LAA+N+PW LD A+ RR EKRI + LP A AR  M    +    +  G    T+ D+ +L + +EGYSG+DI +  ++A M PVRK+  A   +  R      P L +D L T
Sbjct:  239 LASIISRDIYLQNPNVRWDDIIGLDAAKRLVKEAVVYPIKYPQLFTGILSPWKGLLLYGPPGTGKTMLAKAVATEC-KTTFFNISASSIVSKWRGDSEKLVKVLFELARYHAPSTIFLDELESVMGQRGAGGAGGEHEGSRRMKTELLVQMDGLARSDDLVFVLAASNLPWELDHAMLRRLEKRILVDLPSAAARRAMILHHLPPVNSGTGLEIRTELDYEQLAQATEGYSGSDILLVCKEAAMRPVRKIFDA--LESHRPAEEALPALCLDTLHT 511          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Sea Lamprey
Match: fignl1 (fidgetin-like 1 [Source:ZFIN;Acc:ZDB-GENE-030131-1862])

HSP 1 Score: 211.46 bits (537), Expect = 1.968e-60
Identity = 119/324 (36.73%), Postives = 186/324 (57.41%), Query Frame = 2
Query:  389 LNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDND-NVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            +N+ ++   P V WDD+AGL  AK  +KE V+ P+  P +FTG R P KGILL+GPPGTGK+ + K +A+++  + F   +S     KW+GE EK+V+ LF +AR ++P++IFIDEIDSL S R D + +S++RIKTEFLVQ+ G ++  D  VL++ ATN P  +D A RRR  KR+Y+ LP+A AR  + +  +   P    L+ ++   + +RSEG+SGAD++   R+A + P+R V                P++   D L                       + P + + D  +AL   +P+V+ +DL+  +++   F
Sbjct:  378 INNEVMDHGPPVSWDDIAGLEFAKATIKEIVVWPMLRPDIFTGLRGPPKGILLFGPPGTGKTLIGKCIASQSGATFFSISASCLTS-KWVGEGEKMVRALFAVARCRQPAVIFIDEIDSLLSQRADGEHDSSRRIKTEFLVQLDGATTARDERVLLVGATNRPQEIDEAARRRLAKRLYVPLPDAAARRCLLERLLA--PQSWQLSAEELAAVVERSEGFSGADVTQLCREAALGPIRAV----------------PDITTVDALQ----------------------VRP-ILIQDFAQALQVVRPSVSPRDLDLYVEWNRTF 659          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Sea Lamprey
Match: spast (spastin [Source:NCBI gene;Acc:405851])

HSP 1 Score: 207.223 bits (526), Expect = 2.457e-60
Identity = 120/244 (49.18%), Postives = 172/244 (70.49%), Query Frame = 2
Query:  377 LQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGV-SSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKV 1105
            L S + + IV    +V WDD+AG   AK+AL+E VILP   P LFTG R P KG+LL+GPPG GK+ LAKAVA+E+ ++TFF++S+++L SK++GE EKLV+ LF +ARE +PSIIFIDE+DS+   R + + E+++R+KTEFLV+  GV SS  D VL++ ATN P  LD A+ RRF KRIY++LPE    T +  +S+  K   + L+  D   L + +EGYSG+D++   +DA + P+R++
Sbjct:  187 LASLILNEIVDSVLSVQWDDIAGQELAKQALQEIVILPSLRPELFTGLRAPAKGLLLFGPPGNGKTMLAKAVASES-SATFFNISAASLTSKYVGEGEKLVRALFSVARELQPSIIFIDEVDSVLCERREGEHEASRRLKTEFLVEFDGVQSSGEDRVLVMGATNRPQELDEAVLRRFSKRIYVSLPEEEVPTAI-AVSLSWKHA-NPLSHADRAHLARITEGYSGSDLTALAKDAALGPIREL 427          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007508.1 (pep scaffold:Pmarinus_7.0:GL478692:23769:36372:1 gene:ENSPMAG00000006768.1 transcript:ENSPMAT00000007508.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 206.838 bits (525), Expect = 4.687e-60
Identity = 114/238 (47.90%), Postives = 167/238 (70.17%), Query Frame = 2
Query:  401 IVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSD-NDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFK--LSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKV 1105
            IV   P V WDD+AG   AK+ L+E VILP   P LFTG R+P +G+LL+GPPG GK+ LAKAVATE  ++TFF++S+S+++SK++GE EKL++ LF +ARE +PSIIFIDEIDSL   R + + ++++R+KTEFL+Q  GV S+  D VL++ ATN P  LD A+ RRF KRIY+ +P+  AR ++    LS    P    L++++   L + + GYS +D++   +DA + P+R++
Sbjct:  211 IVDRSPQVKWDDIAGQKVAKQTLQEIVILPSLRPELFTGLRSPARGLLLFGPPGNGKTLLAKAVATEC-SATFFNISASSMMSKYVGEGEKLMRALFAVAREMQPSIIFIDEIDSLLCERKEGEHDTSRRMKTEFLMQFDGVKSEAEDRVLVMGATNRPQELDEAVLRRFPKRIYVDMPDEAARKQILNHLLSKHGSP----LSEKELAVLARTTTGYSASDLTALAKDAALGPIREL 443          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Yeast
Match: VPS4 (AAA-ATPase involved in multivesicular body (MVB) protein sorting; ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism [Source:SGD;Acc:S000006377])

HSP 1 Score: 436.032 bits (1120), Expect = 2.508e-149
Identity = 235/437 (53.78%), Postives = 305/437 (69.79%), Query Frame = 2
Query:   50 IQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXX-XXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            + K IE+V KA + D    ++EA   Y +G++Y + ++KY+ +  KSKD IRAK  +YL RAE+LK+ L +E     K   S AG G+N   K     + +     + KKL+  L+ AI+ EKPNV W+DVAGL  AKEALKEAVILP+KFPHLF G R P  GILLYGPPGTGKSYLAKAVATEAN++ F   SS  +  KW+GESEKLVK LF +ARE KPSIIFIDE+D+L   RG+ +SE+++RIKTE LVQM GV +D+  VL+L ATNIPW LDSAIRRRFE+RIYI LP+  ART MF++++G+ P    LT++D+  LG  +EGYSG+DI++ V+DALM P+RK+Q+ATHFK          +      LTPCSPGD  AI M+W  +E ++L EP +T+ D +KA+   +PTVN+ DL K  QFT DF
Sbjct:    7 LTKGIELVQKAIDLDTATQYEEAYTAYYNGLDYLMLALKYEKNP-KSKDLIRAKFTEYLNRAEQLKKHLESEEANAAKKSPS-AGSGSNGGNKKISQEEGEDNGGEDNKKLRGALSSAILSEKPNVKWEDVAGLEGAKEALKEAVILPVKFPHLFKGNRKPTSGILLYGPPGTGKSYLAKAVATEANSTFFSVSSSDLVS-KWMGESEKLVKQLFAMARENKPSIIFIDEVDALTGTRGEGESEASRRIKTELLVQMNGVGNDSQGVLVLGATNIPWQLDSAIRRRFERRIYIPLPDLAARTTMFEINVGDTP--CVLTKEDYRTLGAMTEGYSGSDIAVVVKDALMQPIRKIQSATHFKDVSTEDDETRK------LTPCSPGDDGAIEMSWTDIEADELKEPDLTIKDFLKAIKSTRPTVNEDDLLKQEQFTRDF 432          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Yeast
Match: SAP1 (Putative ATPase of the AAA family; interacts with the Sin1p transcriptional repressor in the two-hybrid system [Source:SGD;Acc:S000000849])

HSP 1 Score: 225.328 bits (573), Expect = 2.965e-64
Identity = 134/258 (51.94%), Postives = 179/258 (69.38%), Query Frame = 2
Query:  386 QLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGD-NDSESAKRIKTEFLVQMQGVSS-------------------DNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVR 1099
            Q+   IVV    VHWDD+AGL +AK +LKEAV+ P   P LF G R P +G+LL+GPPGTGK+ LA+AVATE+ +STFFS+S+S+L SK+LGESEKLV+ LF +A++  PSIIF+DEIDS+  +R + N++ES++RIK EFLVQ   +SS                   D+  VL+LAATN+PW++D A RRRF +R YI LPE   R   FK  + ++  +HTLT+ DF EL K +EGYSG+DI+   +DA M P+R
Sbjct:  590 QIFAEIVVHGDEVHWDDIAGLESAKYSLKEAVVYPFLRPDLFRGLREPVRGMLLFGPPGTGKTMLARAVATES-HSTFFSISASSLTSKYLGESEKLVRALFAIAKKLSPSIIFVDEIDSIMGSRNNENENESSRRIKNEFLVQWSSLSSAAAGSNKSNTNNSDTNGDEDDTRVLVLAATNLPWSIDEAARRRFVRRQYIPLPEDQTRHVQFKKLLSHQ--KHTLTESDFDELVKITEGYSGSDITSLAKDAAMGPLR 844          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Yeast
Match: YTA6 (Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress [Source:SGD;Acc:S000005995])

HSP 1 Score: 219.935 bits (559), Expect = 5.051e-63
Identity = 124/249 (49.80%), Postives = 178/249 (71.49%), Query Frame = 2
Query:  386 QLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSS------DNDN-----VLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVR 1099
            Q+ + I+V    V+W+D+AGL NAK +LKEAV+ P   P LF G R P +G+LL+GPPGTGK+ +AKAVATE+ NSTFFSVS+S+L+SK+LGESEKLV+ LF++A++  PSIIFIDEIDS+ + R DN++ES++RIKTE L+Q   +SS      D +N     VL+L ATN+PWA+D A RRRF +++YI LP+   R    K  M  +  +++L   D+  + + +EG+SG+D++   ++A M P+R
Sbjct:  456 QILNEILVTDEKVYWEDIAGLRNAKNSLKEAVVYPFLRPDLFKGLREPVRGMLLFGPPGTGKTMIAKAVATES-NSTFFSVSASSLLSKYLGESEKLVRALFYMAKKLSPSIIFIDEIDSMLTARSDNENESSRRIKTELLIQWSSLSSATAQSEDRNNTLDSRVLVLGATNLPWAIDDAARRRFSRKLYIPLPDYETRLYHLKRLMAKQ--KNSLQDLDYELITEMTEGFSGSDLTSLAKEAAMEPIR 701          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Yeast
Match: CDC48 (AAA ATPase; subunit of polyUb-selective segregase complex involved in ERAD, INM-associated degradation (INMAD), mitotic spindle disassembly, macroautophagy, PMN, ribosome-associated degradation, ribophagy, homotypic ER membrane fusion, SCF complex disassembly, cell wall integrity during heat stress, and telomerase regulation; mobilizes membrane-anchored transcription factors by regulated Ub/proteasome-dependent processing (RUP); human ortholog VCP complements a cdc48 mutant [Source:SGD;Acc:S000002284])

HSP 1 Score: 149.058 bits (375), Expect = 2.990e-38
Identity = 88/238 (36.97%), Postives = 135/238 (56.72%), Query Frame = 2
Query:  404 VVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTG-KRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSE---SAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRR--RFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVR 1099
            VVE  NV WDDV GL   KE LKE V  P+  P  +T    +P KG+L YGPPGTGK+ LAKAVATE + +         L   W GESE  ++++F  AR   P+++F+DE+DS+   RG +  +   ++ R+  + L +M G+++   NV ++ ATN P  +D AI R  R ++ IY+ LP+  AR  +    +   P +  L   +   + K ++G+SGAD+   V+ A  + ++
Sbjct:  478 VVESVNVTWDDVGGLDEIKEELKETVEYPVLHPDQYTKFGLSPSKGVLFYGPPGTGKTLLAKAVATEVSANFISVKGPELLSM-WYGESESNIRDIFDKARAAAPTVVFLDELDSIAKARGGSLGDAGGASDRVVNQLLTEMDGMNA-KKNVFVIGATNRPDQIDPAILRPGRLDQLIYVPLPDENARLSILNAQLRKTPLEPGL---ELTAIAKATQGFSGADLLYIVQRAAKYAIK 710          

HSP 2 Score: 137.887 bits (346), Expect = 1.581e-34
Identity = 81/230 (35.22%), Postives = 125/230 (54.35%), Query Frame = 2
Query:  422 VHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRT-PWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRR--RFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRK 1102
            V +DD+ G       ++E V LP++ P LF      P +G+L+YGPPGTGK+ +A+AVA E     F       +  K  GESE  ++  F  A +  P+IIFIDEIDS+   R   + E  +R+ ++ L  M G+ +   NV+++AATN P ++D A+RR  RF++ + I +P+A  R E+ ++   N          D   L   + GY GADI+    +A M  +R+
Sbjct:  211 VGYDDIGGCRKQMAQIREMVELPLRHPQLFKAIGIKPPRGVLMYGPPGTGKTLMARAVANETGAFFFLINGPEVMS-KMAGESESNLRKAFEEAEKNAPAIIFIDEIDSIAPKRDKTNGEVERRVVSQLLTLMDGMKA-RSNVVVIAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEVLRIHTKN---MKLADDVDLEALAAETHGYVGADIASLCSEAAMQQIRE 435          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Yeast
Match: PEX6 (AAA-peroxin; heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; mutations in human PEX6 can lead to severe peroxisomal disorders and early death [Source:SGD;Acc:S000005273])

HSP 1 Score: 140.584 bits (353), Expect = 2.146e-35
Identity = 88/237 (37.13%), Postives = 135/237 (56.96%), Query Frame = 2
Query:  416 PNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESA--KRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRR--RFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQD--FVELGKRSE-GYSGADISIAVRDALMFPVRKV 1105
            PNV WDD+ G+   K  + + + +P+K P LFT       GIL YGPPGTGK+ +AKA+AT   +  FFSV    L++ ++GESE  V+ +F  ARE KP +IF DEIDS+   RG+         RI ++ L ++ G+S+D D V ++ ATN P  LD A+ R  RF+K +Y+ +P+    T+  +L++     +  +   D   +EL K     Y+GAD      DA++  + ++
Sbjct:  727 PNVTWDDIGGIDFVKGEILDTIDMPLKHPELFTSGMKKRSGILFYGPPGTGKTLMAKAIATN-FSLNFFSVKGPELLNMYIGESEANVRRVFQKAREAKPCVIFFDEIDSVAPKRGNQGDSGGVMDRIVSQLLAELDGMSTDADGVFVIGATNRPDLLDEALLRPGRFDKLLYLGIPD----TDTKQLNILEALTRKFVLDNDVKLIELAKLCPFNYTGADFYALCSDAMLNAMSRI 958          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Nematostella
Match: EDO35907 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SK75])

HSP 1 Score: 569.311 bits (1466), Expect = 0.000e+0
Identity = 295/445 (66.29%), Postives = 357/445 (80.22%), Query Frame = 2
Query:   35 MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXX----XXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            M+  T+QKAI++VTKATEEDK  N++EALKLYEHGVEYFLH+IKY++  +K+K+SIR+KC QYL+RAEKLK +L      +K  +K +   G++  K     + D +     + E KKL+ QLN AIV+EKPNV W D+AGL +AKEALKEAVILPIKFPHLFTGKRTPW+GILLYGPPGTGKSYLAKAVATEANNSTF SVSSS+LVSKWLGESE+LVK LF LARE KPSIIFIDE+DSLC +R +N+SESA+RIKTEFLVQMQGV  DND VL+L ATNIPW LDSAIRRRFEKRIYI LPE  AR++MF+L +G    +  L  Q+  +LG++++GYSGADIS+ VR+ALM PVRKVQ ATHFKR RGPSP NP+ I DDLLTPCSPGD  AI MNW  +  +KLLEP V++ DM+++L   +PTVN++DL+K  +FT DF
Sbjct:    1 MSGGTLQKAIDLVTKATEEDKAGNYEEALKLYEHGVEYFLHAIKYEAQGDKAKESIRSKCFQYLDRAEKLKTYLR-----QKDKKKPVKAGGSSSTKTGGGKDDDDEDSDSDNAESKKLKGQLNSAIVMEKPNVKWSDIAGLESAKEALKEAVILPIKFPHLFTGKRTPWRGILLYGPPGTGKSYLAKAVATEANNSTFISVSSSDLVSKWLGESERLVKQLFELARENKPSIIFIDEVDSLCGSRSENESESARRIKTEFLVQMQGVGVDNDQVLVLGATNIPWTLDSAIRRRFEKRIYIPLPEQAARSKMFELHLGGS--KTLLGAQEIKQLGQKTDGYSGADISVVVREALMMPVRKVQQATHFKRVRGPSPLNPDEIQDDLLTPCSPGDSGAIEMNWMDVPSDKLLEPGVSMGDMLRSLVTTRPTVNEQDLKKFEKFTADF 438          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Nematostella
Match: EDO31428 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SXZ8])

HSP 1 Score: 244.202 bits (622), Expect = 1.755e-76
Identity = 133/294 (45.24%), Postives = 193/294 (65.65%), Query Frame = 2
Query:  485 LPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKP-SIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVS--SDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            +P++FPHLFTG R PW+ +LLYGPPGTGK+ LA+AVA+E  NSTF+SVSS++L+S W+GESEKL++ LFH AR+++  S+IFIDEIDS+C  R   + E  +R+KTE L QM+G    S +    +L ATN PW LDSA  RRF+KRIYI LPE  AR  + K+ +G  P    LT++D+  LG+++ G+SG+D++    DA+  PVR++Q +TH+K+  G S             PCS G+P  +    + +  +K+    V L D +++L+ N  T+  +DL+K   FT  +
Sbjct:    1 MPLQFPHLFTGGRKPWRRVLLYGPPGTGKTRLAQAVASEV-NSTFYSVSSADLISSWVGESEKLIRELFHDARKREGRSVIFIDEIDSVCRKRSTREEEHTRRVKTELLNQMEGTDSLSLSGQYFLLCATNCPWELDSAFIRRFQKRIYIPLPEQDARISLIKMHLGTTPA--CLTERDWCVLGEKTRGFSGSDLANCTSDAVFEPVRELQRSTHWKQQAGKSA-----------PPCSEGEPGCVTCLLKDLPPQKVTPRPVVLEDFIRSLSHNGSTITDEDLDKFTVFTKSY 280          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Nematostella
Match: KATNA1 (Katanin p60 ATPase-containing subunit A1 [Source:UniProtKB/TrEMBL;Acc:A7RMS0])

HSP 1 Score: 227.254 bits (578), Expect = 1.345e-67
Identity = 128/250 (51.20%), Postives = 171/250 (68.40%), Query Frame = 2
Query:  377 LQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRG-DNDSESAKRIKTEFLVQMQGVSSDNDN-----VLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFV--ELGKRSEGYSGADISIAVRDALMFPVRK 1102
            L   L   I+ + PNVHW D+A L  AK+ L+EAV+LP+  P  F G R PW+G+L+ GPPGTGK+ LAKAVATE   +TFF+VSSS L SK+ GESEKLV+ LF +AR   PS IF+DEIDS+CS RG D++ E+++R+K+E LVQM GV   +D+     V++LAATN PW LD A+RRR EKRIYI LP A  R E+ K+++     +     +D +  E+  + +GYSGADI+   RDA M  +R+
Sbjct:  185 LVEALERDILQKNPNVHWADIADLHEAKKLLEEAVVLPLLMPDYFQGIRRPWRGVLMVGPPGTGKTMLAKAVATEC-GTTFFNVSSSTLTSKYRGESEKLVRILFEMARFYAPSTIFVDEIDSICSRRGSDSEHEASRRVKSELLVQMDGVGGSSDDGETKQVMVLAATNFPWDLDEALRRRLEKRIYIPLPTAEGRLELLKINL-----RGVQMSEDVILEEVANKMDGYSGADITNVCRDASMMAMRR 428          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Nematostella
Match: EDO36214 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJD2])

HSP 1 Score: 221.09 bits (562), Expect = 5.901e-67
Identity = 136/291 (46.74%), Postives = 184/291 (63.23%), Query Frame = 2
Query:  377 LQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDN----DSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFK------LSMGNKPGQHTLTQQDFVELGK--RSEGYSGADISIAVRDALMFPVRKVQTATHFKRA-RGPSPRNPELIVDDLLTPCSPGDPDA 1210
            L + ++  I +  P+V WDD+ GL  AK  +KEAV+ PIK+P LFTG  +PWKG+LLYGPPGTGK+ LAKAVATE  N+TFF++S+S++VSKW G+SEKLV+ LF LAR   PS IF+DE++SL S RG      + E + R+KTE LVQM G++  +D V +LAA+N+PW LD A+ RR EKRI + LP   AR  M        +S  +  G    T+ D+  L K  R+EGYSG+DI +  ++A M  VRK+        A R   PR   +++ D   P    D +A
Sbjct:   19 LAAVISRDIYLHNPDVRWDDIIGLDAAKRLVKEAVVYPIKYPQLFTGILSPWKGLLLYGPPGTGKTLLAKAVATEC-NTTFFNISASSIVSKWRGDSEKLVRVLFELARFHAPSTIFLDELESLMSQRGTGGGGAEHEGSLRMKTELLVQMDGLARSDDLVFLLAASNLPWELDHAMLRRLEKRILVDLPTLEARKAMLMQNLPPVISASDGKGVEIRTEIDYDMLAKVTRAEGYSGSDIKLLAKEAAMRKVRKIFDILEGHHAGRSTQPRTWCVVLPD---PVETSDVEA 305          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Nematostella
Match: EDO39006 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBC8])

HSP 1 Score: 154.066 bits (388), Expect = 3.390e-40
Identity = 95/254 (37.40%), Postives = 145/254 (57.09%), Query Frame = 2
Query:  401 IVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFT--GKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDN-DSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRR--RFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRK-VQTAT----HFKRA 1132
            + +E P VHW DV G    K  LKEAV  P+K P  F   G R P +GIL+YGPPG  K+ +A+A+ATE+  +            KW+GESEK V+ +F  AR   PSI+F DE+D++   R     S+   R+ T+ L ++ GV +  D V+ +AATN P  +D A+ R  R ++ IY+ LP    R  + ++ +   P + +L  +D VE   R+EGYSGA+I+   R+A +  +++ +Q  +    HF++A
Sbjct:  298 VALEVPKVHWSDVGGNEMIKRKLKEAVEWPLKHPEAFQRLGIRPP-RGILMYGPPGCSKTLIARALATESGLNFIAIKGPELFS-KWVGESEKAVREVFLKARATAPSIVFFDELDAIAGQRNSTGGSDVNDRVLTQLLTELDGVETLKD-VIFIAATNRPDMIDKALMRPGRVDRLIYVPLPCWDTRRHILEIHLARTPCEGSLDLEDLVE---RTEGYSGAEIAAVCREAALAALQENIQAESVELRHFEKA 545          

HSP 2 Score: 144.821 bits (364), Expect = 4.730e-37
Identity = 85/235 (36.17%), Postives = 133/235 (56.60%), Query Frame = 2
Query:  413 KPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPW-KGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQG--VSSDNDNVLILAATNIPWALDSAIRR--RFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRK 1102
            K  V +  + GL    +A++E + +P+  P LFT    P  +GILLYGP GTGK+ +A+AVA E     F       L  ++ GE+E  ++ +F  A+ K PSI+FIDE+D+LC  R    +E  +R+    L  M G  + S +  V++LAATN P ALD A+RR  RF++ I I +P    R ++    + N P  H+L  +D   L + + GY GAD++ A ++A ++  ++
Sbjct:   21 KKGVSFQSIGGLKTQIQAVREMIEMPLTNPELFTAYGVPPPRGILLYGPSGTGKTMIARAVANETGVHFFCINGPEVLS-RYYGETEARLREIFTEAQNKSPSIVFIDELDALCPRRDKVQNEFERRVVATLLTLMDGMHMKSTDTYVMVLAATNRPDALDPALRRPGRFDREIEIGIPSVTDRRDILVTLLKNVP--HSLHDEDISSLAESAHGYVGADLAAACKEASLYAFKR 252          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Medaka
Match: vps4a (vacuolar protein sorting 4 homolog A [Source:NCBI gene;Acc:101168949])

HSP 1 Score: 569.311 bits (1466), Expect = 0.000e+0
Identity = 289/441 (65.53%), Postives = 357/441 (80.95%), Query Frame = 2
Query:   35 MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            M ++T+QKAI++VTKATEEDK KN++EAL+LY+H VEYFLH+IKY++HS+K+K+SIRAKC+QYL+RAEKLK++L  +    KK  K       ND    G++    +       LQ QL  AIV+EKPNV W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+L+SKWLGESEKLVKNLF LAR+ KPSIIFIDE+DSLC +R +N+ E+ +RIKTE LVQMQGV ++ND VL+L ATNIPW LD+AIRRRFEKRIYI LPE PAR +MF++ +GN P  H L++ D  +L  ++EGYSGADISI VRDA M PVRKVQ+ATHFK+ RGPS  N +++VDDLLTPCSPGDPDA+ M W  +  +KLLEP V ++DM+++L+  +PTVN +DL K+ +F+ DF
Sbjct:    1 MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKCMQYLDRAEKLKDYLKNKDKQGKKPFKEAQSNDKNDSDCEGENPEKKK-------LQEQLMGAIVMEKPNVRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFDLARQHKPSIIFIDEVDSLCGSRNENEGEAVRRIKTELLVQMQGVGNNNDGVLVLGATNIPWVLDAAIRRRFEKRIYIPLPEEPARVQMFRIHLGNTP--HNLSEADLRQLAHKTEGYSGADISIIVRDAFMQPVRKVQSATHFKKVRGPSRSNNQVMVDDLLTPCSPGDPDAMEMTWMDVPSDKLLEPIVCMSDMLRSLSTTRPTVNTEDLFKVKKFSEDF 432          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Medaka
Match: VPS4B (vacuolar protein sorting 4 homolog B [Source:NCBI gene;Acc:101160844])

HSP 1 Score: 552.747 bits (1423), Expect = 0.000e+0
Identity = 292/443 (65.91%), Postives = 355/443 (80.14%), Query Frame = 2
Query:   35 MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKH--EKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            MA+  +QKAI++ +KA +EDKN+N+ EAL+LY+  V+YFLH +KY++  +K+K SIRAKC +YL+RAEKLKE+L  +         ++S + +  N+  +  D +            Q+QL+ AIV+EKPN+ W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFS+SSS+LVSKWLGESEKLVKNLF LARE KPSIIFIDEIDSLC +R +N+SE+A+RIKTEFLVQMQGV +DN+ VL+L ATNIPW LDSAIRRRFEKRIYI LPE  AR  MFKL +G+ P   TLT+ DF  LGK++ GYSGADIS+ VRDALM PVRKVQ+ATHFKR RGPS  +P ++VDDLLTPCSPGDP+AI M W  + GEKLLEP V + DM+++LA  KPTVN++DLEKL +FT DF
Sbjct:    1 MANNNLQKAIDLASKAAQEDKNQNYDEALRLYQAAVQYFLHVVKYEAQGDKAKQSIRAKCAEYLDRAEKLKEYLKKKEKAPPAKPVKESQSDDKGNESDEGDDPEKKK--------FQNQLSGAIVMEKPNIKWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFSLAREHKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGNDNEGVLVLGATNIPWTLDSAIRRRFEKRIYIPLPEEHARAFMFKLHLGSTP--TTLTESDFATLGKKTNGYSGADISVIVRDALMQPVRKVQSATHFKRVRGPSRDDPNVVVDDLLTPCSPGDPNAIEMTWMEVPGEKLLEPVVCMADMLRSLANTKPTVNEQDLEKLKKFTEDF 433          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Medaka
Match: ENSORLT00000001882.2 (vacuolar protein sorting-associated protein 4B [Source:NCBI gene;Acc:101171319])

HSP 1 Score: 464.922 bits (1195), Expect = 2.090e-160
Identity = 255/441 (57.82%), Postives = 315/441 (71.43%), Query Frame = 2
Query:   53 QKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGG------KKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            Q  I++ +KA EEDK KN++EAL+ Y+H V+YFLH +KY++  +++K SIRAKC  YL+RAE+LKE+L  +         K+  +K  + EG N +KK                 + QL+ AIV+EKPN+ W+DVAGL  AKEALKEAVILPIKFPHLFTGKRTPW+GILL+GPPGTGKSYLAKAVATEANNSTFFSVSSS+LVSKWLGESEKLVKNLF LARE +PSIIFIDEIDSLC +R +N+SE+A+RIKTEFLVQMQGV +DN+ +L+L ATNIPW LDSAIRRRFEKRIYI LPE  AR+ MFKL +G+ P  + LT+ DFV LG++++GYSGADIS+ VRDALM PVR+VQ+ATHFK+A                                               DM++++   KPTVN++DLEKL +FT DF
Sbjct:    8 QPPIDLASKAAEEDKAKNYEEALRCYQHAVQYFLHVVKYETQGDRAKQSIRAKCADYLDRAEQLKEYLKKKENQETAKPVKEAGDKGESDEGDNQEKKK---------------FKDQLSGAIVMEKPNIKWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLVSKWLGESEKLVKNLFALAREHRPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGNDNEGILVLGATNIPWTLDSAIRRRFEKRIYIPLPEEHARSSMFKLHLGSTP--NNLTEADFVTLGRKTDGYSGADISVIVRDALMQPVRRVQSATHFKKA-----------------------------------------------DMLRSVGSTKPTVNEQDLEKLKKFTEDF 384          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Medaka
Match: katnal2 (katanin catalytic subunit A1 like 2 [Source:NCBI gene;Acc:101168474])

HSP 1 Score: 229.18 bits (583), Expect = 3.156e-68
Identity = 124/249 (49.80%), Postives = 170/249 (68.27%), Query Frame = 2
Query:  377 LQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDN---DSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKL---SMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKV 1105
            L + ++  I +  PNV W+D+ GL +AK  +KEAV+ PIK+P LFTG  +PWKG+LLYGPPGTGK+ LAKAVATE   +TFF++S+S++VSKW G+SEKLV+ LF LAR   PS IF+DE++S+   RG +   + E ++R+KTE LVQM G+S   D V +LAA+N+PW LD A+ RR EKRI + LP +PAR  M       + +  G    T  D+  L ++ EGYSG+DI +  ++A M  VR V
Sbjct:  184 LAAIISGDIYLHNPNVRWEDIIGLEDAKRLVKEAVVYPIKYPQLFTGILSPWKGLLLYGPPGTGKTLLAKAVATEC-RTTFFNISASSIVSKWRGDSEKLVRVLFELARYHAPSTIFLDELESVMGQRGTSLGGEHEGSRRMKTELLVQMDGLSRSEDLVFVLAASNLPWELDHAMLRRLEKRILVGLPSSPARQAMISHWLPPLSSTGGMELRTSLDYKMLAEQMEGYSGSDIRLVCKEAAMTLVRTV 431          
BLAST of Vacuolar protein sorting 4 vs. Ensembl Medaka
Match: katnal2 (katanin catalytic subunit A1 like 2 [Source:NCBI gene;Acc:101168474])

HSP 1 Score: 223.787 bits (569), Expect = 1.459e-67
Identity = 124/249 (49.80%), Postives = 169/249 (67.87%), Query Frame = 2
Query:  377 LQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDN---DSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKL---SMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKV 1105
            L + ++  I +  PNV W+D+ GL +AK  +KEAV+ PIK+P LFTG  +PWKG+LLYGPPGTGK+ LAKAVATE   +TFF++S+S++VSKW G+SEKLV+ LF LAR   PS IF+DE++S+   RG +   + E ++R+KTE LVQM G+S   D V +LAA+N+PW LD A+ RR EKRI + LP +PAR  M       + +  G    T  D+  L  + EGYSG+DI +  ++A M  VR V
Sbjct:   40 LAAIISGDIYLHNPNVRWEDIIGLEDAKRLVKEAVVYPIKYPQLFTGILSPWKGLLLYGPPGTGKTLLAKAVATEC-RTTFFNISASSIVSKWRGDSEKLVRVLFELARYHAPSTIFLDELESVMGQRGTSLGGEHEGSRRMKTELLVQMDGLSRSEDLVFVLAASNLPWELDHAMLRRLEKRILVGLPSSPARQAMISHWLPPLSSTGGMELRTSLDYKML--QMEGYSGSDIRLVCKEAAMTLVRTV 285          
BLAST of Vacuolar protein sorting 4 vs. Planmine SMEST
Match: SMESG000075482.1 (SMESG000075482.1)

HSP 1 Score: 828.165 bits (2138), Expect = 0.000e+0
Identity = 440/441 (99.77%), Postives = 441/441 (100.00%), Query Frame = 2
Query:   35 MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFL+AEGGGKKKHEKSLAGEGANDKKKNGDSDSDSEKDVEKKKLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEANNSTFFSVSSSNLVSKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF
Sbjct:    1 MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLNAEGGGKKKHEKSLAGEGANDKKKNGDSDSDSEKDVEKKKLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEANNSTFFSVSSSNLVSKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 441          
BLAST of Vacuolar protein sorting 4 vs. Planmine SMEST
Match: SMESG000009448.1 (SMESG000009448.1)

HSP 1 Score: 491.886 bits (1265), Expect = 2.303e-170
Identity = 268/445 (60.22%), Postives = 340/445 (76.40%), Query Frame = 2
Query:   50 IQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSEKSKDSIRAKCIQYLERAEKLKEFLS----AEGGGKKKHEKSLAGEGANXXXXXXXXXXXXXXXXXXXXLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDALMFPVRKVQTATHFKR-----ARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSMEGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDF 1357
            ++K +E+VT+A  EDKNKN++EALKLYEH +EYFLH++KY+S ++K+K+SIRAKC QYL+RAEKLK+FL+    +E G          GE +    K                 +S+L  AIV+EKPN+HW+D+AGL  AKEAL+EAVILP+KFP LFTGKR PW+GILLYGPPGTGKSYLAKAVATEA NSTFF++SSS+LVSKWLGESEKLVKNLF LARE +PSIIFIDEIDS+C +R +++SESA+RIKTEFLVQM+GV + ND VL+LAATNIPWALDSAIRRRFEKRIYI LP+  AR  +F+LS+G+ P  +T+++ D   L KR+EGYSG+DI+  VR+A+M PV+KV +ATHFK+     A    P N    + +  TPCSPGD  A+ M W+ ++ E+LLEP V ++DM+KAL  NKPTVN  DL+KL +FT +F
Sbjct:    3 LEKGVELVTRAAIEDKNKNYEEALKLYEHALEYFLHALKYESPTDKTKESIRAKCTQYLDRAEKLKKFLADKSKSEVGSTGGTTTKKKGEDSELDDKEDVK------------FKSKLAGAIVMEKPNIHWNDIAGLHGAKEALQEAVILPLKFPDLFTGKRKPWRGILLYGPPGTGKSYLAKAVATEAENSTFFAISSSDLVSKWLGESEKLVKNLFELAREHRPSIIFIDEIDSMCGSRSESESESARRIKTEFLVQMEGVGAQNDKVLVLAATNIPWALDSAIRRRFEKRIYIPLPDLGARALLFRLSLGDTP--NTISEADINSLSKRTEGYSGSDIANLVREAIMQPVKKVMSATHFKKVSKEHANKLRPNNKN--ITEYWTPCSPGDKAAVEMTWKDIKAEQLLEPVVNMSDMIKALGSNKPTVNSNDLKKLQEFTEEF 431          
BLAST of Vacuolar protein sorting 4 vs. Planmine SMEST
Match: SMESG000057662.1 (SMESG000057662.1)

HSP 1 Score: 224.172 bits (570), Expect = 1.011e-65
Identity = 124/250 (49.60%), Postives = 176/250 (70.40%), Query Frame = 2
Query:  377 LQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGD---NDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNK---PGQHTL-TQQDFVELGKRSEGYSGADISIAVRDALMFPVRKV 1105
            L   ++  I +  P+V W+D+ GL +AK+ +KEAV+ PIK+P LF G  +PWKGILLYGPPGTGK+ LAKAVATE  N+TFF++S+S++VSKW G+SEKLV+ LF LAR   PS IFIDE++S+ S RG     + E ++R+KTE L+QM G+S  +D V +LAA+N+PW LD+A+ RR EKRI ++LP   AR  +F+  +  K    GQ  L +  D+  L + +EGYSG+DI +  +++ M  +RK+
Sbjct:  223 LAVSISKDIYLHNPDVRWNDIVGLDSAKQLIKEAVVYPIKYPRLFQGILSPWKGILLYGPPGTGKTLLAKAVATEC-NTTFFNISASSIVSKWRGDSEKLVRILFELARFHAPSTIFIDELESVMSQRGSGAGGEHEGSRRMKTELLIQMDGLSKSDDLVFLLAASNLPWELDNAMLRRLEKRILVSLPSKEARQALFQNFLPPKISNEGQLDLRSNIDYALLAENTEGYSGSDIKLVCKESAMKSIRKI 471          
BLAST of Vacuolar protein sorting 4 vs. Planmine SMEST
Match: SMESG000057662.1 (SMESG000057662.1)

HSP 1 Score: 224.172 bits (570), Expect = 1.112e-65
Identity = 123/242 (50.83%), Postives = 173/242 (71.49%), Query Frame = 2
Query:  401 IVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGD---NDSESAKRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNK---PGQHTL-TQQDFVELGKRSEGYSGADISIAVRDALMFPVRKV 1105
            I +  P+V W+D+ GL +AK+ +KEAV+ PIK+P LF G  +PWKGILLYGPPGTGK+ LAKAVATE  N+TFF++S+S++VSKW G+SEKLV+ LF LAR   PS IFIDE++S+ S RG     + E ++R+KTE L+QM G+S  +D V +LAA+N+PW LD+A+ RR EKRI ++LP   AR  +F+  +  K    GQ  L +  D+  L + +EGYSG+DI +  +++ M  +RK+
Sbjct:  238 IYLHNPDVRWNDIVGLDSAKQLIKEAVVYPIKYPRLFQGILSPWKGILLYGPPGTGKTLLAKAVATEC-NTTFFNISASSIVSKWRGDSEKLVRILFELARFHAPSTIFIDELESVMSQRGSGAGGEHEGSRRMKTELLIQMDGLSKSDDLVFLLAASNLPWELDNAMLRRLEKRILVSLPSKEARQALFQNFLPPKISNEGQLDLRSNIDYALLAENTEGYSGSDIKLVCKESAMKSIRKI 478          
BLAST of Vacuolar protein sorting 4 vs. Planmine SMEST
Match: SMESG000069747.1 (SMESG000069747.1)

HSP 1 Score: 211.46 bits (537), Expect = 1.723e-61
Identity = 125/250 (50.00%), Postives = 165/250 (66.00%), Query Frame = 2
Query:  389 LNDAIVVEKPNVHWDDVAGLLNAKEALKEAVILPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEAXXXXXXXXXXXXXXXKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGD-NDSESAKRIKTEFLVQMQGVSSDN-------DNVLILAATNIPWALDSAIRRRFEKRIYIALPEAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSE---GYSGADISIAVRDALMFPVRKV 1105
            L   IV    NV+WDD+A L  AK  LKEA++LP+  P+ FTG R PWKG+L++GPPGTGK+ LAKAVATE   +TFFSV+SS+L SKW GESEKL++ LF +AR   PS IFIDEID++ S R D + +ES +R+ +E L+Q+ G+SS N        +V IL ATN+PW LD A+RRR EKRIYI LP    R  +  +++        +   D V+L   +E   GYSG+DI+   RDA M  +R+V
Sbjct:  175 LEKDIVQRNLNVNWDDIADLDEAKRLLKEAIVLPLLLPNYFTGLRKPWKGVLMFGPPGTGKTLLAKAVATEC-QTTFFSVTSSSLTSKWRGESEKLIRILFDMARFYAPSTIFIDEIDAIGSKRDDGSGNESNRRVMSELLIQIDGISSSNGQEYDATKSVTILGATNLPWDLDEALRRRLEKRIYIPLPGGVGRRVLLDINL------KAIDLSDDVDLDAIAEKLTGYSGSDITQVCRDAAMMQLRRV 417          
The following BLAST results are available for this feature:
BLAST of Vacuolar protein sorting 4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
VPS4A0.000e+066.89vacuolar protein sorting 4 homolog A [Source:HGNC ... [more]
VPS4B0.000e+066.67vacuolar protein sorting 4 homolog B [Source:HGNC ... [more]
AC026464.40.000e+068.64novel protein[more]
KATNA18.000e-6851.81katanin catalytic subunit A1 [Source:HGNC Symbol;A... [more]
KATNA18.000e-6851.81katanin catalytic subunit A1 [Source:HGNC Symbol;A... [more]
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BLAST of Vacuolar protein sorting 4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
vps-41.685e-18062.81Related to yeast Vacuolar Protein Sorting factor ... [more]
spas-14.715e-5938.56Probable spastin homolog spas-1 [Source:UniProtKB... [more]
spas-11.444e-5838.56Probable spastin homolog spas-1 [Source:UniProtKB... [more]
mei-11.546e-5850.00Meiotic spindle formation protein mei-1 [Source:U... [more]
mei-15.689e-5850.22Meiotic spindle formation protein mei-1 [Source:U... [more]
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BLAST of Vacuolar protein sorting 4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Vps40.000e+070.45gene:FBgn0283469 transcript:FBtr0074504[more]
Vps40.000e+070.45gene:FBgn0283469 transcript:FBtr0342787[more]
kat-60L12.805e-6140.72gene:FBgn0037375 transcript:FBtr0300739[more]
kat-60L12.805e-6140.72gene:FBgn0037375 transcript:FBtr0078627[more]
kat-60L13.860e-6140.72gene:FBgn0037375 transcript:FBtr0300741[more]
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BLAST of Vacuolar protein sorting 4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
vps4b0.000e+065.53vacuolar protein sorting 4 homolog B [Source:ZFIN;... [more]
vps4b0.000e+065.53vacuolar protein sorting 4 homolog B [Source:ZFIN;... [more]
vps4a0.000e+067.31vacuolar protein sorting 4 homolog A [Source:ZFIN;... [more]
vps4b1.360e-1465.83vacuolar protein sorting 4 homolog B [Source:ZFIN;... [more]
katnal12.503e-6843.58katanin p60 subunit A-like 1 [Source:NCBI gene;Acc... [more]
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BLAST of Vacuolar protein sorting 4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSXETT00000004853.10.000e+067.12vacuolar protein sorting 4 homolog A [Source:NCBI ... [more]
ENSXETT00000005228.10.000e+067.51vacuolar protein sorting 4 homolog A [Source:NCBI ... [more]
ENSXETT00000005102.10.000e+067.51vacuolar protein sorting 4 homolog A [Source:NCBI ... [more]
ENSXETT00000005911.10.000e+067.21vacuolar protein sorting 4 homolog A [Source:NCBI ... [more]
ENSXETT00000005821.10.000e+067.44vacuolar protein sorting 4 homolog A [Source:NCBI ... [more]
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BLAST of Vacuolar protein sorting 4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Vps4a0.000e+067.12vacuolar protein sorting 4A [Source:MGI Symbol;Acc... [more]
Vps4b0.000e+067.49vacuolar protein sorting 4B [Source:MGI Symbol;Acc... [more]
Vps4b0.000e+067.49vacuolar protein sorting 4B [Source:MGI Symbol;Acc... [more]
Katnal13.626e-6748.25katanin p60 subunit A-like 1 [Source:MGI Symbol;Ac... [more]
Katnal27.934e-6751.04katanin p60 subunit A-like 2 [Source:MGI Symbol;Ac... [more]
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BLAST of Vacuolar protein sorting 4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9UN37|VPS4A_HUMAN0.000e+066.89Vacuolar protein sorting-associated protein 4A OS=... [more]
sp|Q793F9|VPS4A_RAT0.000e+067.12Vacuolar protein sorting-associated protein 4A OS=... [more]
sp|Q8VEJ9|VPS4A_MOUSE0.000e+067.12Vacuolar protein sorting-associated protein 4A OS=... [more]
sp|Q0VD48|VPS4B_BOVIN0.000e+068.76Vacuolar protein sorting-associated protein 4B OS=... [more]
sp|O75351|VPS4B_HUMAN0.000e+066.67Vacuolar protein sorting-associated protein 4B OS=... [more]
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BLAST of Vacuolar protein sorting 4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3J3G60.000e+073.58vacuolar protein sorting-associated protein 4A iso... [more]
A0A1S3J3230.000e+073.12vacuolar protein sorting-associated protein 4A iso... [more]
A0A1S3J3I00.000e+072.89vacuolar protein sorting-associated protein 4A iso... [more]
A0A1S3J4P70.000e+072.89vacuolar protein sorting-associated protein 4A iso... [more]
A0A1S3J3180.000e+072.67vacuolar protein sorting-associated protein 4A iso... [more]
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BLAST of Vacuolar protein sorting 4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
vps4a0.000e+066.06vacuolar protein sorting 4 homolog A [Source:NCBI ... [more]
vps4a0.000e+066.13vacuolar protein sorting 4 homolog A [Source:NCBI ... [more]
vps4a0.000e+066.36vacuolar protein sorting 4 homolog A [Source:NCBI ... [more]
ENSAMXT00000018479.20.000e+065.37vacuolar protein sorting-associated protein 4B-lik... [more]
ENSAMXT00000044404.10.000e+064.92vacuolar protein sorting-associated protein 4B-lik... [more]
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BLAST of Vacuolar protein sorting 4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000003236.10.000e+069.34pep scaffold:Pmarinus_7.0:GL477724:37443:44438:-1 ... [more]
katnal24.549e-6748.19katanin p60 subunit A-like 2 [Source:ZFIN;Acc:ZDB-... [more]
fignl11.968e-6036.73fidgetin-like 1 [Source:ZFIN;Acc:ZDB-GENE-030131-1... [more]
spast2.457e-6049.18spastin [Source:NCBI gene;Acc:405851][more]
ENSPMAT00000007508.14.687e-6047.90pep scaffold:Pmarinus_7.0:GL478692:23769:36372:1 g... [more]
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BLAST of Vacuolar protein sorting 4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
VPS42.508e-14953.78AAA-ATPase involved in multivesicular body (MVB) p... [more]
SAP12.965e-6451.94Putative ATPase of the AAA family; interacts with ... [more]
YTA65.051e-6349.80Putative ATPase of the CDC48/PAS1/SEC18 (AAA) fami... [more]
CDC482.990e-3836.97AAA ATPase; subunit of polyUb-selective segregase ... [more]
PEX62.146e-3537.13AAA-peroxin; heterodimerizes with AAA-peroxin Pex1... [more]
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BLAST of Vacuolar protein sorting 4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO359070.000e+066.29Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO314281.755e-7645.24Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
KATNA11.345e-6751.20Katanin p60 ATPase-containing subunit A1 [Source:... [more]
EDO362145.901e-6746.74Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO390063.390e-4037.40Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Vacuolar protein sorting 4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
vps4a0.000e+065.53vacuolar protein sorting 4 homolog A [Source:NCBI ... [more]
VPS4B0.000e+065.91vacuolar protein sorting 4 homolog B [Source:NCBI ... [more]
ENSORLT00000001882.22.090e-16057.82vacuolar protein sorting-associated protein 4B [So... [more]
katnal23.156e-6849.80katanin catalytic subunit A1 like 2 [Source:NCBI g... [more]
katnal21.459e-6749.80katanin catalytic subunit A1 like 2 [Source:NCBI g... [more]
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BLAST of Vacuolar protein sorting 4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000075482.10.000e+099.77SMESG000075482.1[more]
SMESG000009448.12.303e-17060.22SMESG000009448.1[more]
SMESG000057662.11.011e-6549.60SMESG000057662.1[more]
SMESG000057662.11.112e-6550.83SMESG000057662.1[more]
SMESG000069747.11.723e-6150.00SMESG000069747.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001710 ID=SMED30001710|Name=Vacuolar protein sorting 4|organism=Schmidtea mediterranea sexual|type=transcript|length=1802bp
AATTTATTTATCTTCCTTGAATAATAAATAATTTATGGCTTCAACAACTA
TTCAGAAAGCTATAGAAATTGTTACTAAAGCTACTGAAGAAGATAAAAAT
AAGAATTTCAAAGAAGCTTTGAAGTTGTATGAACACGGAGTAGAATATTT
TCTCCACAGCATTAAATATGACAGTCATTCAGAAAAATCTAAAGACAGTA
TACGTGCAAAATGTATTCAATATTTAGAAAGGGCGGAAAAATTGAAAGAA
TTCTTAAGTGCTGAAGGTGGTGGCAAAAAGAAACACGAAAAATCATTAGC
AGGAGAAGGCGCAAATGATAAAAAGAAAAATGGGGATTCAGATAGTGATA
GCGAAAAAGACGTTGAAAAGAAAAAATTGCAATCACAATTGAATGACGCC
ATAGTAGTGGAAAAGCCAAATGTACATTGGGACGATGTTGCAGGATTGTT
AAATGCAAAAGAAGCCTTAAAGGAAGCTGTGATTTTACCAATTAAATTTC
CTCATTTATTTACTGGTAAACGTACCCCATGGAAAGGAATACTGCTTTAT
GGGCCTCCAGGAACTGGAAAGTCATATTTAGCTAAAGCAGTTGCTACTGA
AGCAAACAATTCAACTTTTTTCTCTGTATCAAGCTCTAATTTAGTTTCAA
AATGGCTGGGAGAAAGTGAAAAGCTCGTTAAGAATCTATTTCATCTGGCT
AGAGAAAAGAAACCTAGCATTATATTTATCGACGAAATCGATTCTCTATG
TAGTAATCGTGGCGATAACGATTCTGAATCAGCTAAACGCATCAAAACAG
AATTTCTCGTTCAAATGCAAGGAGTTTCATCGGACAACGATAACGTCTTG
ATTTTAGCTGCCACTAATATCCCATGGGCTCTTGACTCAGCAATTCGTCG
TCGATTTGAAAAGCGTATCTATATCGCCTTACCTGAAGCTCCTGCTCGTA
CAGAAATGTTCAAATTATCGATGGGAAACAAGCCTGGTCAGCACACTCTC
ACCCAGCAGGACTTTGTGGAGCTTGGCAAGCGATCAGAAGGCTATTCTGG
AGCCGATATCAGCATTGCGGTCCGGGATGCTCTCATGTTCCCAGTGAGAA
AAGTCCAAACCGCAACACATTTCAAGCGAGCCCGAGGTCCCTCGCCTCGC
AATCCAGAGCTAATTGTGGATGATTTATTAACTCCATGTTCACCCGGAGA
TCCGGACGCCATTGCTATGAATTGGCAATCGATGGAGGGTGAGAAATTGT
TGGAGCCAGCAGTTACCTTAAACGACATGATGAAAGCATTGGCCCGCAAC
AAACCAACAGTTAATCAGAAGGATCTCGAGAAGCTGATTCAATTCACCAA
TGATTTCGGCCAGGATGGTTGAGAGGAGCGGGGAGGGGGGGACGCGTTCC
CTTTTGGATTCGCCGTTGTGTCGACAGATATTGAATAAATGCTTACAATT
TTGCGGCGTCGTGTTAAGATTTATGACTTATTTTAAAAATTACAATTTTA
TTGCATTACTGTTTGTGATTGGTTGAAATGATTAGTTCGTATGCGTGCGT
CTGTTTGTGCTTGTATGTACAAATGCATTATACATCTACATTATTGATTA
AATTGATCGATTGGATGAGCTGCCACGTTTCTGTTTACGAATAATACTAA
TAAACGACTTTAATTTTTATTTAATTTACTAAATATTGATTTGAGAACTT
ATTTATAGACTTATAACTTGAATAGTGCTATTGATTATTTTGTATATTAA
TAATTTAGTGAGTGCAATATTTTCATTTTTTACATATTCAATGTTATTTG
TG
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protein sequence of SMED30001710-orf-1

>SMED30001710-orf-1 ID=SMED30001710-orf-1|Name=SMED30001710-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=446bp
MASTTIQKAIEIVTKATEEDKNKNFKEALKLYEHGVEYFLHSIKYDSHSE
KSKDSIRAKCIQYLERAEKLKEFLSAEGGGKKKHEKSLAGEGANDKKKNG
DSDSDSEKDVEKKKLQSQLNDAIVVEKPNVHWDDVAGLLNAKEALKEAVI
LPIKFPHLFTGKRTPWKGILLYGPPGTGKSYLAKAVATEANNSTFFSVSS
SNLVSKWLGESEKLVKNLFHLAREKKPSIIFIDEIDSLCSNRGDNDSESA
KRIKTEFLVQMQGVSSDNDNVLILAATNIPWALDSAIRRRFEKRIYIALP
EAPARTEMFKLSMGNKPGQHTLTQQDFVELGKRSEGYSGADISIAVRDAL
MFPVRKVQTATHFKRARGPSPRNPELIVDDLLTPCSPGDPDAIAMNWQSM
EGEKLLEPAVTLNDMMKALARNKPTVNQKDLEKLIQFTNDFGQDG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR041569AAA_lid_3
IPR036181MIT_dom_sf
IPR003593AAA+_ATPase
IPR007330MIT
IPR015415Vps4_C
IPR003959ATPase_AAA_core
IPR027417P-loop_NTPase
IPR003960ATPase_AAA_CS
Vocabulary: molecular function
TermDefinition
GO:0005524ATP binding
GO:0000166nucleotide binding
GO:0005515protein binding
GO:0008022protein C-terminus binding
GO:0016787hydrolase activity
GO:0016887ATPase activity
GO:0019904protein domain specific binding
GO:0042623ATPase activity, coupled
Vocabulary: biological process
TermDefinition
GO:0000920cell separation after cytokinesis
GO:0006810transport
GO:0006900membrane budding
GO:0006997nucleus organization
GO:0007033vacuole organization
GO:0007049cell cycle
GO:0007080mitotic metaphase plate congression
GO:0009838abscission
GO:0015031protein transport
GO:0016192vesicle-mediated transport
GO:0016197endosomal transport
GO:0016236macroautophagy
GO:0019058viral life cycle
GO:0019076viral release from host cell
GO:0032367intracellular cholesterol transport
GO:0032466negative regulation of cytokinesis
GO:0032880regulation of protein localization
GO:0034058endosomal vesicle fusion
GO:0036258multivesicular body assembly
GO:0039702viral budding via host ESCRT complex
GO:0043162ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
GO:0051301cell division
GO:0061738late endosomal microautophagy
GO:0072319vesicle uncoating
GO:0090611ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
GO:1902188positive regulation of viral release from host cell
GO:1903076regulation of protein localization to plasma membrane
GO:1903543positive regulation of exosomal secretion
GO:1903774positive regulation of viral budding via host ESCRT complex
GO:1903902positive regulation of viral life cycle
GO:1904896ESCRT complex disassembly
GO:1904903ESCRT III complex disassembly
Vocabulary: cellular component
TermDefinition
GO:0000922spindle pole
GO:0005634nucleus
GO:0005737cytoplasm
GO:0005764lysosome
GO:0005768endosome
GO:0005769early endosome
GO:0005770late endosome
GO:0005774vacuolar membrane
GO:0005813centrosome
GO:0005829cytosol
GO:0005886plasma membrane
GO:0016020membrane
GO:0030496midbody
GO:0031902late endosome membrane
GO:0048471perinuclear region of cytoplasm
GO:0070062extracellular exosome
GO:0090543Flemming body
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003593AAA+ ATPase domainSMARTSM00382AAA_5coord: 165..301
e-value: 1.6E-20
score: 84.2
IPR007330MITSMARTSM00745smartcoord: 2..80
e-value: 3.7E-22
score: 89.6
IPR007330MITPFAMPF04212MITcoord: 6..72
e-value: 1.8E-22
score: 79.1
IPR041569AAA ATPase, AAA+ lid domainPFAMPF17862AAA_lid_3coord: 326..357
e-value: 2.6E-6
score: 27.1
IPR015415Vps4 oligomerisation, C-terminalPFAMPF09336Vps4_Ccoord: 382..442
e-value: 7.1E-28
score: 96.4
NoneNo IPR availableGENE3DG3DSA:1.20.58.280coord: 4..76
e-value: 1.1E-29
score: 104.1
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 128..434
e-value: 4.5E-134
score: 448.4
NoneNo IPR availableGENE3DG3DSA:1.10.8.60coord: 301..425
e-value: 4.5E-134
score: 448.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..112
NoneNo IPR availablePANTHERPTHR23074AAA ATPASEcoord: 7..443
NoneNo IPR availablePANTHERPTHR23074:SF83VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4Acoord: 7..443
NoneNo IPR availableCDDcd00009AAAcoord: 136..299
e-value: 2.41449E-28
score: 107.232
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 169..298
e-value: 1.5E-41
score: 141.9
IPR003960ATPase, AAA-type, conserved sitePROSITEPS00674AAAcoord: 271..290
IPR036181MIT domain superfamilySUPERFAMILYSSF116846MIT domaincoord: 4..81
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 128..436