DNA-directed RNA polymerase subunit
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30001693 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Human
Match: POLR1A (RNA polymerase I subunit A [Source:HGNC Symbol;Acc:HGNC:17264]) HSP 1 Score: 576.63 bits (1485), Expect = 1.700e-177 Identity = 388/1093 (35.50%), Postives = 566/1093 (51.78%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQ---------------GLLESICDLGQIKDSLIKRFND------YENMKSKTLSDKQKNALKIMSSQTR--------------------KNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYGKFTNTDTSIFPEILREHLRLLWSNESENLQLILPMLKYNNF--KYPTDVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREII-------------------KRKDS-TAAEQYYQNIREG----------ILELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPMVIGHNPLEVVENLKFQL--------QNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW-----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYL-VYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLM----EFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKN-NLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQ-LMEKRYLKEPRNTKPVLSKVLAKK 3074
L I F Y+ EE++ LS K IT+ ++ LG + NGLYD +LG + KE C C F +C+GH G IE VYNPLLFD L ++ C CH L + G L+++ +L +I + ++ D E ++ T Q N L + + K C C V +K ++T+ A R G+ + + I+E +Q G T T REHL LW NE L + + + ++ VFF+ + L+ PP P GDQM + +T N+ V++ +R+++ + KDS A ++ + + G + LQ VN+ +DS + + PG+RQ ++KK+GLFR ++MGKRV++A RSVI PD I +EIG+P+VF +KLTYP+P+ P N L Q V+NGP+V+PGA V + G+R + + E + QL + T I+ RH +GD L++NRQPTLHRPS+QAH + + P+ K LR+HY+NCKAYNADFDGDEMN H+PQS L +AEA +L Q+ PKDG PLAGLIQDH+++G M R FTR ++LVY GL + R KL P+I+ P LWTGKQ++S +L N IP N+S W R + + ++II +G + G+ DK H G S GL E+YG E + K+LT L+RL T YL +Y GF+ ++D + + R+ I+ + G +V + N+P E RG+ + + Q D ID FK ++Y +++ + C L +FP+N+L +M Q+GAKG N +I+ LG IE+EG+R PL G++LP F+ Y+ P + GF RF++G+KP E FF MAGR+GLVDTAVKTS SGYLQRC+IKHLE + V +D TVRD+ +++QF YG DGLD+ + L +P +A N + +M+ ++L E VLS+ KK
Sbjct: 12 LQGISFGMYSAEELKKLSVKSITNPRYLDS-LGNPSANGLYDLALGPADSKEVCSTCVQDFSNCSGHLGHIELPLTVYNPLLFDKLYLLLRGSCLNCHMLTCPRAVIHLLLCQLRVLEVGALQAVYELERILNRFLEENPDPSASEIREELEQYTTEIVQNNLLGSQGAHVKNVCESKSKLIALFWKAHMNAKRCPHCKTGRSVVRKEHNSKLTITFPAMVHRTAGQKDSEPLGIEE-----------AQIGKRGYLTPTSA-------REHLSALWKNEGFFLNYLFSGMDDDGMESRFNPSVFFL-DFLVVPPSRYRPVSRLGDQMFTNGQTVNLQAVMKDVVLIRKLLALMAQEQKLPEEVATPTTDEEKDSLIAIDRSFLSTLPGQSLIDKLYNIWIRLQSHVNIVFDSEMDKLMMDKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSR--TALSAVDMTQREAVAKQLLTPATGAPKPQGTKIVCRHVKNGDILLLNRQPTLHRPSIQAHRARIL--PEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGASMTTRGCFFTREHYMELVYRGLTDKVGR-VKLLSPSILKPFPLWTGKQVVSTLLINIIPEDHIPLNLSGKAKITGKAWVKETPRSVPGFNPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGKVLTCLARLFTAYLQLYRGFTLGVEDILVKPKADVKRQRIIEESTHCGPQAVRAALNLPEAASYDEVRGKWQDAHLGKDQRD--------FNMIDLKFKEEVNHYSNEINKACMPFGLHRQFPENSLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFL-QPKQFPFLASNYEVIMKSQHLHE------VLSRADPKK 1064 HSP 2 Score: 137.502 bits (345), Expect = 9.643e-32 Identity = 83/199 (41.71%), Postives = 116/199 (58.29%), Query Frame = 3
Query: 3141 LREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQLPFKITVGD------------YYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKKTFKQTLINITDNKDEDNQAVQTDLVNEDELN 3701
LR ++ +K + S+C GEAVGLLAAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPRLREILM+ S IKTP M VP+L A+ S+ K+ T++ L +++Q KI V + Y + F F Y+ + L+ ++++ E F + +++KK K + N + +A Q DL N EL
Sbjct: 1192 LRTLLQLKWQRSLCEPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKALKRVKSLKKQLTRVCLGEVLQ---KIDVQESFCMEEKQNKFQVYQLRFQFLPHAYYQQEKCLRPEDILRFMETRFFKLLMESIKK--KNNKASAFRNVN-TRRATQRDLDNAGELG 1384 HSP 3 Score: 124.02 bits (310), Expect = 1.217e-27 Identity = 62/169 (36.69%), Postives = 101/169 (59.76%), Query Frame = 3
Query: 4005 FDVKSILLNIFENCVISSVAGVEKALIFNSNKSKR-----FATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
FD+ S+++++ VI + G+ + L+ + +K TEG N ELFKY + DL + YSN+I ++ YGIEAA+ IE EIK VF Y I V+ RHL L+AD++ G NR + ++SSPLQ+ +FET+ + + G+ D ++SP++ ++ G++V
Sbjct: 1477 FDMSSLVVSLAHGAVIYATKGITRCLLNETTNNKNEKELVLNTEGINLPELFKYAEVLDLRRLYSNDIHAIANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIRSNSSPLQQMTFETSFQFLKQATMLGSHDELRSPSACLVVGKVV 1645
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Human
Match: POLR1A (RNA polymerase I subunit A [Source:HGNC Symbol;Acc:HGNC:17264]) HSP 1 Score: 575.859 bits (1483), Expect = 9.861e-177 Identity = 388/1093 (35.50%), Postives = 566/1093 (51.78%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQ---------------GLLESICDLGQIKDSLIKRFND------YENMKSKTLSDKQKNALKIMSSQTR--------------------KNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYGKFTNTDTSIFPEILREHLRLLWSNESENLQLILPMLKYNNF--KYPTDVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREII-------------------KRKDS-TAAEQYYQNIREG----------ILELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPMVIGHNPLEVVENLKFQL--------QNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW-----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYL-VYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLM----EFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKN-NLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQ-LMEKRYLKEPRNTKPVLSKVLAKK 3074
L I F Y+ EE++ LS K IT+ ++ LG + NGLYD +LG + KE C C F +C+GH G IE VYNPLLFD L ++ C CH L + G L+++ +L +I + ++ D E ++ T Q N L + + K C C V +K ++T+ A R G+ + + I+E +Q G T T REHL LW NE L + + + ++ VFF+ + L+ PP P GDQM + +T N+ V++ +R+++ + KDS A ++ + + G + LQ VN+ +DS + + PG+RQ ++KK+GLFR ++MGKRV++A RSVI PD I +EIG+P+VF +KLTYP+P+ P N L Q V+NGP+V+PGA V + G+R + + E + QL + T I+ RH +GD L++NRQPTLHRPS+QAH + + P+ K LR+HY+NCKAYNADFDGDEMN H+PQS L +AEA +L Q+ PKDG PLAGLIQDH+++G M R FTR ++LVY GL + R KL P+I+ P LWTGKQ++S +L N IP N+S W R + + ++II +G + G+ DK H G S GL E+YG E + K+LT L+RL T YL +Y GF+ ++D + + R+ I+ + G +V + N+P E RG+ + + Q D ID FK ++Y +++ + C L +FP+N+L +M Q+GAKG N +I+ LG IE+EG+R PL G++LP F+ Y+ P + GF RF++G+KP E FF MAGR+GLVDTAVKTS SGYLQRC+IKHLE + V +D TVRD+ +++QF YG DGLD+ + L +P +A N + +M+ ++L E VLS+ KK
Sbjct: 12 LQGISFGMYSAEELKKLSVKSITNPRYLDS-LGNPSANGLYDLALGPADSKEVCSTCVQDFSNCSGHLGHIELPLTVYNPLLFDKLYLLLRGSCLNCHMLTCPRAVIHLLLCQLRVLEVGALQAVYELERILNRFLEENPDPSASEIREELEQYTTEIVQNNLLGSQGAHVKNVCESKSKLIALFWKAHMNAKRCPHCKTGRSVVRKEHNSKLTITFPAMVHRTAGQKDSEPLGIEE-----------AQIGKRGYLTPTSA-------REHLSALWKNEGFFLNYLFSGMDDDGMESRFNPSVFFL-DFLVVPPSRYRPVSRLGDQMFTNGQTVNLQAVMKDVVLIRKLLALMAQEQKLPEEVATPTTDEEKDSLIAIDRSFLSTLPGQSLIDKLYNIWIRLQSHVNIVFDSEMDKLMMDKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSR--TALSAVDMTQREAVAKQLLTPATGAPKPQGTKIVCRHVKNGDILLLNRQPTLHRPSIQAHRARIL--PEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGASMTTRGCFFTREHYMELVYRGLTDKVGR-VKLLSPSILKPFPLWTGKQVVSTLLINIIPEDHIPLNLSGKAKITGKAWVKETPRSVPGFNPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGKVLTCLARLFTAYLQLYRGFTLGVEDILVKPKADVKRQRIIEESTHCGPQAVRAALNLPEAASYDEVRGKWQDAHLGKDQRD--------FNMIDLKFKEEVNHYSNEINKACMPFGLHRQFPENSLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFL-QPKQFPFLASNYEVIMKSQHLHE------VLSRADPKK 1064 HSP 2 Score: 137.502 bits (345), Expect = 1.053e-31 Identity = 83/199 (41.71%), Postives = 116/199 (58.29%), Query Frame = 3
Query: 3141 LREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQLPFKITVGD------------YYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKKTFKQTLINITDNKDEDNQAVQTDLVNEDELN 3701
LR ++ +K + S+C GEAVGLLAAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPRLREILM+ S IKTP M VP+L A+ S+ K+ T++ L +++Q KI V + Y + F F Y+ + L+ ++++ E F + +++KK K + N + +A Q DL N EL
Sbjct: 1192 LRTLLQLKWQRSLCEPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKALKRVKSLKKQLTRVCLGEVLQ---KIDVQESFCMEEKQNKFQVYQLRFQFLPHAYYQQEKCLRPEDILRFMETRFFKLLMESIKK--KNNKASAFRNVN-TRRATQRDLDNAGELG 1384 HSP 3 Score: 124.405 bits (311), Expect = 1.138e-27 Identity = 62/169 (36.69%), Postives = 101/169 (59.76%), Query Frame = 3
Query: 4005 FDVKSILLNIFENCVISSVAGVEKALIFNSNKSKR-----FATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
FD+ S+++++ VI + G+ + L+ + +K TEG N ELFKY + DL + YSN+I ++ YGIEAA+ IE EIK VF Y I V+ RHL L+AD++ G NR + ++SSPLQ+ +FET+ + + G+ D ++SP++ ++ G++V
Sbjct: 1538 FDMSSLVVSLAHGAVIYATKGITRCLLNETTNNKNEKELVLNTEGINLPELFKYAEVLDLRRLYSNDIHAIANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIRSNSSPLQQMTFETSFQFLKQATMLGSHDELRSPSACLVVGKVV 1706
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Human
Match: POLR3A (RNA polymerase III subunit A [Source:HGNC Symbol;Acc:HGNC:30074]) HSP 1 Score: 386.726 bits (992), Expect = 2.759e-111 Identity = 351/1203 (29.18%), Postives = 548/1203 (45.55%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITS--LISIE----PVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKI-MSSQTRKN--CHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQ-EELYGKFTNTDTSIFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAVMNG--DQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDSGRHRELTG---------SQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQ-----------IGNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQR-EFKLPKPAIIFPNQLWTGKQILSIVLK----NSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQK-GLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKD-----GFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLD---------------VINSV----------SLSKPDFM---EVIAKNIQLM--EKRYLKEPRNTKPVLSKVLAKKIDELVV--------KLKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDI 3446
+ ICF +PEE+R + ++ S L S + P+L G+ D +G+ PC C + DC GH+G I+ ++ F + ++ +C C + +Q K+F DY +K L+ QK LK +S + RK CH C + KK +I +H K ++ +P ++ F+++ +E+ ++ P ++ L L +E++ L+L + P+D+ L+ PP+ P+V++ E T + ++ L ++IK+ + A+ Q I E LQ + + +S EL+G G Q++ K G FR N+ GKRV+F+ R+VISPDP + +DE+ VP+ LT+PE +N N +FL + V NGP+V+PGA Q GNR M + LK+ I+ RH + GD ++ NRQP+LH+ S+ AH+ + +T R + C YNADFDGDEMN H PQ+ A+AEA +L+ TP++G PL IQD + + L+D+ F R + ++ L +++ + +LP P I+ P LWTGKQI S++L+ N + A L + + G + L +D + I + + +G DK LG K + L +G A ++ L+RL YL GFS I D G L E I + + GK L + G A +E + R+ C LRE N+ L M G+KG N++++ +G I G R P R+LP F+++ P ++GF + F SGL P E FF +MAGR+GLVDTAVKT+ +GY+QR L+K LED+ +D TVR + +IIQF YG DGLD V++++ +LSK + + E I K + + + +L+E + +S+ + K D+ + ++ I+P E K + G AVG L AQS+GEP TQMTL TFH AG + MN+TLG+PR++EI+ S I TP + + DDA + A V + K L +I
Sbjct: 15 ISHICFGMKSPEEMRQQAHIQVVSKNLYSQDNQHAPLL-----YGVLDHRMGTSEKDRPCETCGKNLADCLGHYGYIDLELPCFHVGYFRAVIGILQMICKTCCHIMLSQ--------------EEKKQFLDY--LKRPGLTYLQKRGLKKKISDKCRKKNICHHCGAFNGTVKKCGLLKI-IHEKYKTNKKVVDPIVSNF-------LQSFETAIEHNKEVEPLLGRAQENLNPLVV---LNLFKRIPAEDVPLLLMNPEAGK---PSDLILTR--LLVPPLCIRPSVVSDLKSGTNEDDLTMKLTEII----FLNDVIKKHRISGAKT--QMIMEDWDFLQLQCALYINS----ELSGIPLNMAPKKWTRGFVQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLRIDEVAVPVHVAKILTFPEKVNKANINFLRKLVQNGPEVHPGANFIQQRHTQMKRFLKYGNREKM---------AQELKY------GDIVERHLIDGDVVLFNRQPSLHKLSIMAHLARV---KPHRTFRFNECVCTPYNADFDGDEMNLHLPQTEEAKAEALVLMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTFFDRAKACQIIASILVGKDEKIKVRLPPPTILKPVTLWTGKQIFSVILRPSDDNPVRANL-----------RTKGKQYCGKGEDLCANDSYVTIQNSELMSGSMDKGTLGSGSKNNIFYILLRDWGQNEAADAMSRLARLAPVYLSNRGFSIGIGDVTPGQGLLKAKYELLNAGYKKCDEYIEALNTGK-----LQQQPGCTAEETLEALILKELSVIRDHAG------------------SAC---LRELDKSNSPLTMALCGSKGSFINISQMIACVGQQAISGSRVPDGFENRSLPHFEKHSKLPAAKGFVANSFYSGLTPTEFFFHTMAGREGLVDTAVKTAETGYMQRRLVKSLEDLCSQYDLTVRSSTGDIIQFIYGGDGLDPAAMEGKDEPLEFKRVLDNIKAVFPCPSEPALSKNELILTTESIMKKSEFLCCQDSFLQEIKKFIKGVSEKIKKTRDKYGINDNGTTEPRVLYQLDRITPTQVEKFLETCRDKYMRAQMEPGSAVGALCAQSIGEPGTQMTLKTFHFAGVASMNITLGVPRIKEIIN-ASKAISTPIITAQLDKDDDA-DYARLVKGRIEKTLLGEI 1113 HSP 2 Score: 57.7658 bits (138), Expect = 2.238e-7 Identity = 44/161 (27.33%), Postives = 76/161 (47.20%), Query Frame = 3
Query: 4047 VISSVAGVEKALIF---NSNKSK-RFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTN-HSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTN 4514
V+ + V +A+I S K K + EG N + + + SNN ++ + GIEAA + I NEI+ ++ + ++ RH+ L++D +T+ G+V R + S L ASFE + + +G D + + I+ G + NIGT
Sbjct: 1200 VVQGIPEVSRAVIHIDEQSGKEKYKLLVEGDNLRAVMATHGVKG-TRTTSNNTYEVEKTLGIEAARTTIINEIQYTMVNHGMSIDRRHVMLLSDLMTYKGEVLGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMG-IPMNIGTG 1358
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Human
Match: POLR2A (RNA polymerase II subunit A [Source:HGNC Symbol;Acc:HGNC:9187]) HSP 1 Score: 351.673 bits (901), Expect = 4.122e-98 Identity = 292/972 (30.04%), Postives = 454/972 (46.71%), Query Frame = 3
Query: 90 ICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNC----HQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYG--KFTNTDTS-----IFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAV-MNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDS---GRHRELTGS-QP--GLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQI---GNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEI--DTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGL 2936
+ F +P+E++ +S E G+ + GL D G + C+ C + +C GHFG IE +K V++ K ++ VCF+C +L L++S + +IKD L +K+ +K + KN + NK V + ++T ++KG C R + P+IR S ELY K N D+ + PE + E + + E +L M +Y + + +L PP++ PAV M G + T + ++V+ LR R + A + I E + LQ V D+ G R + S +P L+Q++ K+G R N+MGKRV+F+ R+VI+PDP + +D++GVP + +T+ E + P N D L + V G YPGA Y I G+R + P ++ LQ V RH GD +I NRQPTLH+ SM H ++ + T R++ S YNADFDGDEMN H PQSL +AE + L V TP+ P+ G++QD + A K RD R E ++L L + + K+P+PAI+ P LWTGKQI S++ IP + + P SG + + D ++++ +G + GI K LG S L G ++ +N+ ++ +L+ G + I D + +N + K + + K+ N E +L + F N++ I D KT SS + +L E NN M +GAKG + N++++ +G +EGKR P RTLP F + D P SRGF + +++GL P E FF +M GR+GL+DTAVKT+ +GY+QR LIK +E + V +D TVR++++ ++Q RYG DGL
Sbjct: 21 VQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKL-----LVDS--NNPKIKDIL-----------AKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLT--------KEKGHGGCGRYQ----PRIR-----RSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEEC----FVLGM----EPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLR----RNEQNGAAAHV--IAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGA-KYIIRDNGDRIDLRFHPKPSDL------HLQTGYKV--ERHMCDGDIVIFNRQPTLHKMSMMGHRVRIL---PWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNL----LMFLSTWDGKVPQPAILKPRPLWTGKQIFSLI----IPGHINCIRTHSTHPDDED----SGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHN---NELEPTPGNTLRQ--------TFENQVNRILNDARDKTGSS---------AQKSLSEY---NNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGL 896 HSP 2 Score: 81.2629 bits (199), Expect = 1.537e-14 Identity = 47/80 (58.75%), Postives = 58/80 (72.50%), Query Frame = 3
Query: 3138 LLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILA 3377
LL EI + K +I GE VG LAAQS+GEP+TQMTLNTFH AG S NVTLG+PRL+E++ + S K KTPS+ V +L
Sbjct: 1068 LLGEIES-KFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINI-SKKPKTPSLTVFLLG 1145 HSP 3 Score: 68.9366 bits (167), Expect = 8.833e-11 Identity = 41/128 (32.03%), Postives = 66/128 (51.56%), Query Frame = 3
Query: 4116 TEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNH-SSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
T+G + + + + D + SN+I+++ + GIEA +E E+ V VN RHL L+ D +T G + A RH + + PL + SFE + V++E +G D +K + NIM GQL
Sbjct: 1338 TDGVSLMRVLSEKDV-DPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLA 1464
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Human
Match: POLR2A (RNA polymerase II subunit A [Source:HGNC Symbol;Acc:HGNC:9187]) HSP 1 Score: 351.673 bits (901), Expect = 4.122e-98 Identity = 292/972 (30.04%), Postives = 454/972 (46.71%), Query Frame = 3
Query: 90 ICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNC----HQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYG--KFTNTDTS-----IFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAV-MNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDS---GRHRELTGS-QP--GLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQI---GNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEI--DTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGL 2936
+ F +P+E++ +S E G+ + GL D G + C+ C + +C GHFG IE +K V++ K ++ VCF+C +L L++S + +IKD L +K+ +K + KN + NK V + ++T ++KG C R + P+IR S ELY K N D+ + PE + E + + E +L M +Y + + +L PP++ PAV M G + T + ++V+ LR R + A + I E + LQ V D+ G R + S +P L+Q++ K+G R N+MGKRV+F+ R+VI+PDP + +D++GVP + +T+ E + P N D L + V G YPGA Y I G+R + P ++ LQ V RH GD +I NRQPTLH+ SM H ++ + T R++ S YNADFDGDEMN H PQSL +AE + L V TP+ P+ G++QD + A K RD R E ++L L + + K+P+PAI+ P LWTGKQI S++ IP + + P SG + + D ++++ +G + GI K LG S L G ++ +N+ ++ +L+ G + I D + +N + K + + K+ N E +L + F N++ I D KT SS + +L E NN M +GAKG + N++++ +G +EGKR P RTLP F + D P SRGF + +++GL P E FF +M GR+GL+DTAVKT+ +GY+QR LIK +E + V +D TVR++++ ++Q RYG DGL
Sbjct: 21 VQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKL-----LVDS--NNPKIKDIL-----------AKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLT--------KEKGHGGCGRYQ----PRIR-----RSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEEC----FVLGM----EPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLR----RNEQNGAAAHV--IAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGA-KYIIRDNGDRIDLRFHPKPSDL------HLQTGYKV--ERHMCDGDIVIFNRQPTLHKMSMMGHRVRIL---PWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNL----LMFLSTWDGKVPQPAILKPRPLWTGKQIFSLI----IPGHINCIRTHSTHPDDED----SGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHN---NELEPTPGNTLRQ--------TFENQVNRILNDARDKTGSS---------AQKSLSEY---NNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGL 896 HSP 2 Score: 81.2629 bits (199), Expect = 1.537e-14 Identity = 47/80 (58.75%), Postives = 58/80 (72.50%), Query Frame = 3
Query: 3138 LLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILA 3377
LL EI + K +I GE VG LAAQS+GEP+TQMTLNTFH AG S NVTLG+PRL+E++ + S K KTPS+ V +L
Sbjct: 1068 LLGEIES-KFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINI-SKKPKTPSLTVFLLG 1145 HSP 3 Score: 68.9366 bits (167), Expect = 8.833e-11 Identity = 41/128 (32.03%), Postives = 66/128 (51.56%), Query Frame = 3
Query: 4116 TEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNH-SSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
T+G + + + + D + SN+I+++ + GIEA +E E+ V VN RHL L+ D +T G + A RH + + PL + SFE + V++E +G D +K + NIM GQL
Sbjct: 1338 TDGVSLMRVLSEKDV-DPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLA 1464
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Celegans
Match: rpoa-1 (DNA-directed RNA polymerase subunit [Source:UniProtKB/TrEMBL;Acc:G5EDG7]) HSP 1 Score: 500.745 bits (1288), Expect = 7.181e-150 Identity = 288/762 (37.80%), Postives = 431/762 (56.56%), Query Frame = 3
Query: 762 LREHLRLLWSNESENLQLILPML----KYNNFKYPTDVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREI--IKRKDSTA-----------AEQYYQNIREGILELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNP------LEVVENLKFQLQNN--QTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISG-DEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETC-KNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSK 2966
+REH R+L+ N+ + L + PML + P D F+E +L+ PP P M E P+T N+ V+ A + I I + D++A + + + L+LQ R N +D ++ S G++Q ++KK GLFR ++MGKRVNFACRSVI+PDP + +DEIG+P +F KLT+ EP+N N + + + GP +PGA + + ++G P +++ + L N QT + RH +GD ++MNRQP+LH+PS+ H + + +A LRM+Y+ CKAYNADFDGDEMNGH QS +AQ E + + NV PKD +PL GLIQDH+++G + +RD + + + LV ++R ++P P I++P +LWTGKQ+++ ++KN IP G L N+ + W + G D + +S+ ++ G + G+ DK H G +Q GL ELYG +++L+ SRL T YL + GF+ + D + + + RK + + R+IG+ V +F +P E +L + KM +D K + Y + ++C L FP N L LM Q+GAKG N +I+ LG IE+EGKR +T+ GRTLPSF+ +D P + G+ RF++G+ PQELFF +MAGR+GL+DTAVKTS SGYLQRC+IKHLE I V +D TVRD+ ++IQFRYG DG+D + L+K
Sbjct: 315 VREHFRMLFKNDGKLLLKLFPMLVDELNGEDMICPLDGLFLERILV-PPKKFRPIRMFKGAQYEDPQTLNLRKVLEATETISAISLIMKGDTSAQLKELIANRVRGKTINAQMHDAYLQLQLRANAIFDQDLNKGDRDSIAGIKQILEKKQGLFRMHMMGKRVNFACRSVITPDPYLDIDEIGIPDIFAKKLTFTEPVNAFNVNEMKGLLRQGPHQHPGANFFVEPSGKKTMLGDKPEEKKRRMQMAKTLNAATTENLRQTPKVLRHMKNGDMIMMNRQPSLHKPSILGHRARVLTGQRA--LRMNYAPCKAYNADFDGDEMNGHLVQSHIAQTEVREIANVGSNFLVPKDATPLLGLIQDHVVSGVLLSVRDRFLNKEDFMHLVLASFAQYSKR-IEIPPPTILYPKRLWTGKQVITTIVKNCIPDGKPLINLDGKAKTPLSCWIVPGFDAPQFDMSESHVVFRQGELLVGVLDKAHFGATQFGLAHCAFELYGHRCGVQLLSCFSRLFTTYLQFHGFTLGVADILVVKDADGKRKEAVMESRTIGNQVVKTAFGLPDTATPAEIKRTLAATYCNPRGQGTDVKM--LDFGMKQGIAKYNDAITKSCVPTGLLRLFPQNALQLMIQSGAKGSAVNAIQISGCLGQIELEGKRMAVTIAGRTLPSFRCFDPSPRAGGYIDQRFLTGMNPQELFFHTMAGREGLIDTAVKTSRSGYLQRCIIKHLEGIRVHYDSTVRDHDGSVIQFRYGEDGMDTTKATFLNK 1070 HSP 2 Score: 117.472 bits (293), Expect = 5.079e-26 Identity = 60/182 (32.97%), Postives = 99/182 (54.40%), Query Frame = 3
Query: 3969 WIKLYLNL---NHKRFDVKSILLNIFENCVISSVAGVEKALIFNSNKSKR----FATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQL 4493
W ++ L N + DV SI+ E ++ G+E+ + K+ + T+G N FK+ + D+N YSN++ + YG+EA I E+ VF Y I+V+ RHL L AD++T +G++ FNR M++ SSPLQ+ +FET + + E L +G D + SP++ ++ G L
Sbjct: 1519 WCEVVFELPLRNKTKMDVSSIVEKEVELFIVHQTPGIERCVETTEQKNGKEMTILQTQGVNLAAFFKHADVLDVNSVYSNDLNLILENYGVEACSKAITTEMNNVFAVYGIEVSKRHLSLTADYMTFTGQIQPFNRGAMSSSSSPLQKMTFETTMAFLREALLHGEEDNVNSPSARLVMGAL 1700 HSP 3 Score: 114.775 bits (286), Expect = 3.518e-25 Identity = 79/210 (37.62%), Postives = 116/210 (55.24%), Query Frame = 3
Query: 3051 LSKVLAKKIDEL----VVKLKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQ---LPFKITVGD------YYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKKTFKQTLINITD 3641
L + K +DE+ ++K+ E PK+ +L+ + K S+ GE VGLLAAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPRLREILM S I TPS + ++A + + S+ ++ ++YLK +++ L KIT+ Y++ + LKR +++ E FI A+ +KK + + ITD
Sbjct: 1184 LGALPEKMLDEIEGFCTRRVKKVDDEEPPKE--VLKRTLYWKGMRSLADPGENVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMTASKSIATPSASIAVIA-GTSRDRIDSIKRELDRVYLKQLLKNFSLEEKITLTQNQSCRRYHLRIDILAAEKRELGARHLKRSQIMEEIEKRFILRVAQAIKKKYHE----ITD 1386 HSP 4 Score: 70.0922 bits (170), Expect = 1.438e-11 Identity = 36/92 (39.13%), Postives = 46/92 (50.00%), Query Frame = 3
Query: 105 YTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRL 380
Y P E+ LS +IT + + V G GLYD LG N + C CN C GH G I+ + V+NPLLF +K C +CHRL
Sbjct: 23 YFPHEIDKLSVLKITQTKTFDEV-GHPIAGGLYDPILGPDNTFDMCMTCNQYERHCPGHMGHIQLAVPVFNPLLFQFTYNLLKGSCVHCHRL 113
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Celegans
Match: rpc-1 (DNA-directed RNA polymerase subunit [Source:UniProtKB/TrEMBL;Acc:Q18566]) HSP 1 Score: 361.688 bits (927), Expect = 3.846e-103 Identity = 334/1198 (27.88%), Postives = 533/1198 (44.49%), Query Frame = 3
Query: 195 GLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFN-DYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYGKFTNTD--TSIFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAVMNGDQM--TESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDSGRHRELTGSQPGLR---------QKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA---VSYQIGNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNS--NQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQK-GLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKD-----GFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLD--VINSVSLSKPDFMEVI--AKNIQLMEKRYLKEPRNTKPV--LSKVLAKKIDELVVKLKRDFVEISPKDENLLREI----------------------IAVKSEYSICPNGEA-------------------------------------VGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINS--ATSVLKKFTKLYLKDIIQLPFKITVGDYYIITFTFKNK 3512
G D +G C C L DC GHFG + V++ F + + ++ +C C + T + ++ + N DY + K L + A K S+ C C K+ KK+ + + ++ S D+ + F + +S + G T + P +++ L+SN E +L M++ K+P D+ + PP+ P+V++ + TE T + ++ L++ ++D ++ ++ L++Q + + + E++G P ++ Q++ K G FR N+ GKRV+F+ R+VISPDP + +D++GVPI LT+PE +N N + + Q V+NG DV+PGA V + GN+ + G E+ +NL+ I RH D ++ NRQP+LH+ S+ +H K + +T R + C YNADFDGDEMN H PQ+ A+AEA L+NV TP+ G PL IQD I G + +D+ R E + S Q + ++P PAI P +LWTGKQ++ ++++ + ++S + KN + +G+ + L D +II + + G DK LG S K + L YG + A+ + L+R+ +L GFS I D L E G+ + I GK P G +E + R+ ++ C NL + N L M G+KG N++++ +G I G R P R+LP F+R P ++GF + F SGL P E FF +M GR+GLVDTAVKT+ +GY+QR L+K LED+ +DGTVR ++ ++I+F +G DGLD ++ + S DF V+ AKNIQ +++ + PV L +VL +I + K K +V + K + + + K IC N EA VG +AA S+GEPSTQMTL TFH AG + MN+T G+PR++EI+ + +KT S + A D + A V + K L +I ++ + D Y + +K
Sbjct: 55 GPLDPKMGVSTKTGTCSTCGLGLTDCVGHFGYFDLDVPVFHIGFFKLTIQLLQCICKNCSSILLTP-------EQHRVFSRQVMNPNLDY--LHRKALHKRIVAACKKGST-----CSHCGLKNGTVKKAVGAVLKIAFASPVSVDE---------------LGKFATMFSSNQEVGDHVRKMKFTLLNPLFVQK----LFSNIKEGDIPVL-MVRSGEEKHPNDLLLTR--MPVPPVCIRPSVVSEVKAGTTEDDVTMKLMEIMLTNDVLKK--HKRDGAPSKTLFETWEH--LQIQCALYI------NSEMSGLPPDMQPKRAMRAFTQRLKGKQGRFRGNLCGKRVDFSGRTVISPDPNLRIDQVGVPIHVAMTLTFPEIVNASNIEKMRQLVINGSDVHPGANYLVEKKTGNKKLLKYGKRD-ELAKNLRM------GDTIERHLDDNDVVLFNRQPSLHKISIMSHRAKVM---PGRTFRFNECACTPYNADFDGDEMNLHLPQTYEAKAEASELMNVKNNLITPRSGEPLVAAIQDFITGGYLLTHKDTFLPRAEVYRFAAALIDASAKKQTKIRIPPPAIRRPVELWTGKQLIELIIRPD-----KGSDVSLNLTAKNKSY--TGNLE-LCSKDSYVIIRNSVLLAGCLDKSLLGSSSKVNIFYMLMRDYGEDAAVDAMWRLARMAPVFLSNRGFSIGIGDVRPSEQLLREKGQLVDNGYSKCSQYIKELEEGKLKAQP-----GCTEEETLESIILRELSTIRDHAGQV------------------CLRNLSKY---NAPLTMAVCGSKGSFINISQMIACVGQQAISGHRPPDGFEERSLPHFERKKKTPEAKGFVANSFYSGLTPTEFFFHTMGGREGLVDTAVKTAETGYMQRRLVKCLEDLCASYDGTVRSSVGDVIEFVFGEDGLDPAMMEAKDGSVVDFTHVLEHAKNIQTTKEQPI-------PVDKLDEVLKAEIQK---KFKGKYVHFADKLSEYITQTEIKKSKKWQNGKAHCGNHETADLKTKESCKICKNLEAYKTSLLANSCLTKTQLCSFIELCYYKVARAITEPGTAVGAIAATSIGEPSTQMTLKTFHFAGVASMNITQGVPRIKEII----NAVKTISTPIITAALLDPYDESLARRVKARIEKTTLGEICDYIEEVYLPDDYFLLVKLNSK 1148 HSP 2 Score: 62.7734 bits (151), Expect = 2.322e-9 Identity = 48/169 (28.40%), Postives = 80/169 (47.34%), Query Frame = 3
Query: 4020 ILLNIFENCVISSVAGVEKALIFNSNKSKRFAT---EGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRH-MMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTN 4514
IL N V+ + V + +I K F + EG + + + D K NN + ++ + GIEAA SCI NEI ++ I ++ RH+ L+AD +T+ G+V R+ ++ S L ASFE + + E + D I + I+ G ++ +GT
Sbjct: 1206 ILKYSLANVVVKGIPSVNRCVIHADEKKGDFYSLLVEGTDFRSVLSSVGV-DPRKTNFNNALVVADVLGIEAARSCIINEIIATMDAHGIGLDRRHVMLLADVMTYRGEVLGITRNGLVKMKDSVLLLASFEKTMDHLFEAAFFSQRDVIHGVSECIIMGTPMT-VGTG 1372
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Celegans
Match: ama-1 (DNA-directed RNA polymerase II subunit RPB1 [Source:UniProtKB/Swiss-Prot;Acc:P16356]) HSP 1 Score: 345.51 bits (885), Expect = 1.292e-96 Identity = 269/955 (28.17%), Postives = 434/955 (45.45%), Query Frame = 3
Query: 90 ICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYGKFTNTDTSIFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAVMN-GDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDSGRHRELTGSQP--GLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEI--DTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLD 2939
+ F PEE++ +S + E GK + GL D G ++ + C C + DC GHFG +E +K V++ K ++ VCFYC RL + D R E +K + K++ + + + C K + + +PS +R+ ID N + + + +QE +F +I E + L++ M + ++ + + +L PP+ PAV+ G + T + ++++ L+ + AA ++R + V+ C G +P ++Q++ K+G R N+MGKRV+F+ R+VI+ DP + +D +GVP LT+PE + P N D L + V G YPGA N + + ++P LQ V RH GD ++ NRQPTLH+ SM H +K + T RM+ S YNADFDGDEMN H PQSL +AE + + V Q TP+ P+ G++QD + A M RD +DL+ + + K+P+PAI+ P LWTGKQ+ S++ IP + + + P SG + + D ++II G + +GI +G S L + G E+A +++ ++ +L+ G + I D +++ +N + K + + K+ N L G F NK+ +I D +T SS + +L E NN M +G+KG + N++++ +G +EGKR P RTLP F + D P S+GF + +++GL P E FF +M GR+GL+DTAVKT+ +GY+QR LIK +E + V +DGTVR+++ ++Q RYG DGLD
Sbjct: 18 VQFGILGPEEIKRMSVAHVEFPEVYE--NGKPKLGGLMDPRQGVIDRRGRCMTCAGNLTDCPGHFGHLELAKPVFHIGFLTKTLKILRCVCFYCGRL---------------LIDKSAPRV--LEILKKTGTNSKKRLTMIYDLCKAKSVCEGAAEKEEGMPDDPDDPMNDGKKVAGGCGRYQPSYRRVGIDINAEWKKNVNEDTQERKIMLTAERVLEVFQQITDEDI------------LVIGM----DPQFARPEWMICTVLPVPPLAVRPAVVTFGSAKNQDDLTHKLSDIIKTNQQLQR--NEANGAAAHVLTDDVRLLQFHVATLVDNCIPGLPTATQKGGRPLKSIKQRLKGKEGRIRGNLMGKRVDFSARTVITADPNLPIDTVGVPRTIAQNLTFPEIVTPFNVDKLQELVNRGDTQYPGAKYIIRENGARVDLRYHPRAA----DLHLQPGYRV--ERHMKDGDIIVFNRQPTLHKMSMMGHRVKIL---PWSTFRMNLSVTSPYNADFDGDEMNLHLPQSLETRAEIEEIAMVPRQLITPQANKPVMGIVQDTLCAVRMMTKRDVFIDWPFMMDLLMY----LPTWDGKVPQPAILKPKPLWTGKQVFSLI----IPGNVNVLRTHSTHPDSED----SGPYKWISPGDTKVIIEHGELLSGIVCSKTVGKSAGNLLHVVTLELGYEIAANFYSHIQTVINAWLIREGHTIGIGDTIADQATYLDIQNTIRKAKQDVVDVIEKAHNDDLEPTPGNTL-----------RQTFENKVNQILNDARDRTGSS---------AQKSLSEF---NNFKSMVVSGSKGSKINISQVIACVGQQNVEGKRIPFGFRHRTLPHFIKDDYGPESKGFVENSYLAGLTPSEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKAMESVMVNYDGTVRNSLAQMVQLRYGEDGLD 891 HSP 2 Score: 81.6481 bits (200), Expect = 4.879e-15 Identity = 45/76 (59.21%), Postives = 57/76 (75.00%), Query Frame = 3
Query: 3138 LLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDV 3365
LL EI + + + +I GE VG LAAQS+GEP+TQMTLNTFH AG S NVTLG+PRL+EI+ + S +KTPS+ V
Sbjct: 1062 LLGEIES-RFQQAIAQPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKEIINV-SKTLKTPSLTV 1135 HSP 3 Score: 66.2402 bits (160), Expect = 2.293e-10 Identity = 44/138 (31.88%), Postives = 63/138 (45.65%), Query Frame = 3
Query: 4086 FNSNKSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHS-SPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
F S T+G L + I D + SN+I ++ + GIEA IE E+ V VN RHL L+ D +T G + A RH + L R SFE + +++E + D +K + NIM GQL
Sbjct: 1320 FKSVADWILETDGTALLRVLSERQI-DPVRTTSNDICEIFEVLGIEAVRKAIEREMDNVISFDGSYVNYRHLALLCDVMTAKGHLMAITRHGINRQEVGALMRCSFEETVDILMEAAVHAEEDPVKGVSENIMLGQLA 1456
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Celegans
Match: ama-1 (DNA-directed RNA polymerase II subunit RPB1 [Source:UniProtKB/Swiss-Prot;Acc:P16356]) HSP 1 Score: 345.51 bits (885), Expect = 1.292e-96 Identity = 269/955 (28.17%), Postives = 434/955 (45.45%), Query Frame = 3
Query: 90 ICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYGKFTNTDTSIFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAVMN-GDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDSGRHRELTGSQP--GLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEI--DTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLD 2939
+ F PEE++ +S + E GK + GL D G ++ + C C + DC GHFG +E +K V++ K ++ VCFYC RL + D R E +K + K++ + + + C K + + +PS +R+ ID N + + + +QE +F +I E + L++ M + ++ + + +L PP+ PAV+ G + T + ++++ L+ + AA ++R + V+ C G +P ++Q++ K+G R N+MGKRV+F+ R+VI+ DP + +D +GVP LT+PE + P N D L + V G YPGA N + + ++P LQ V RH GD ++ NRQPTLH+ SM H +K + T RM+ S YNADFDGDEMN H PQSL +AE + + V Q TP+ P+ G++QD + A M RD +DL+ + + K+P+PAI+ P LWTGKQ+ S++ IP + + + P SG + + D ++II G + +GI +G S L + G E+A +++ ++ +L+ G + I D +++ +N + K + + K+ N L G F NK+ +I D +T SS + +L E NN M +G+KG + N++++ +G +EGKR P RTLP F + D P S+GF + +++GL P E FF +M GR+GL+DTAVKT+ +GY+QR LIK +E + V +DGTVR+++ ++Q RYG DGLD
Sbjct: 18 VQFGILGPEEIKRMSVAHVEFPEVYE--NGKPKLGGLMDPRQGVIDRRGRCMTCAGNLTDCPGHFGHLELAKPVFHIGFLTKTLKILRCVCFYCGRL---------------LIDKSAPRV--LEILKKTGTNSKKRLTMIYDLCKAKSVCEGAAEKEEGMPDDPDDPMNDGKKVAGGCGRYQPSYRRVGIDINAEWKKNVNEDTQERKIMLTAERVLEVFQQITDEDI------------LVIGM----DPQFARPEWMICTVLPVPPLAVRPAVVTFGSAKNQDDLTHKLSDIIKTNQQLQR--NEANGAAAHVLTDDVRLLQFHVATLVDNCIPGLPTATQKGGRPLKSIKQRLKGKEGRIRGNLMGKRVDFSARTVITADPNLPIDTVGVPRTIAQNLTFPEIVTPFNVDKLQELVNRGDTQYPGAKYIIRENGARVDLRYHPRAA----DLHLQPGYRV--ERHMKDGDIIVFNRQPTLHKMSMMGHRVKIL---PWSTFRMNLSVTSPYNADFDGDEMNLHLPQSLETRAEIEEIAMVPRQLITPQANKPVMGIVQDTLCAVRMMTKRDVFIDWPFMMDLLMY----LPTWDGKVPQPAILKPKPLWTGKQVFSLI----IPGNVNVLRTHSTHPDSED----SGPYKWISPGDTKVIIEHGELLSGIVCSKTVGKSAGNLLHVVTLELGYEIAANFYSHIQTVINAWLIREGHTIGIGDTIADQATYLDIQNTIRKAKQDVVDVIEKAHNDDLEPTPGNTL-----------RQTFENKVNQILNDARDRTGSS---------AQKSLSEF---NNFKSMVVSGSKGSKINISQVIACVGQQNVEGKRIPFGFRHRTLPHFIKDDYGPESKGFVENSYLAGLTPSEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKAMESVMVNYDGTVRNSLAQMVQLRYGEDGLD 891 HSP 2 Score: 81.6481 bits (200), Expect = 4.879e-15 Identity = 45/76 (59.21%), Postives = 57/76 (75.00%), Query Frame = 3
Query: 3138 LLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDV 3365
LL EI + + + +I GE VG LAAQS+GEP+TQMTLNTFH AG S NVTLG+PRL+EI+ + S +KTPS+ V
Sbjct: 1062 LLGEIES-RFQQAIAQPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKEIINV-SKTLKTPSLTV 1135 HSP 3 Score: 66.2402 bits (160), Expect = 2.293e-10 Identity = 44/138 (31.88%), Postives = 63/138 (45.65%), Query Frame = 3
Query: 4086 FNSNKSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHS-SPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
F S T+G L + I D + SN+I ++ + GIEA IE E+ V VN RHL L+ D +T G + A RH + L R SFE + +++E + D +K + NIM GQL
Sbjct: 1320 FKSVADWILETDGTALLRVLSERQI-DPVRTTSNDICEIFEVLGIEAVRKAIEREMDNVISFDGSYVNYRHLALLCDVMTAKGHLMAITRHGINRQEVGALMRCSFEETVDILMEAAVHAEEDPVKGVSENIMLGQLA 1456
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Celegans
Match: rpoa-1 (DNA-directed RNA polymerase subunit [Source:UniProtKB/TrEMBL;Acc:G5EDG7]) HSP 1 Score: 77.7962 bits (190), Expect = 1.458e-16 Identity = 34/82 (41.46%), Postives = 54/82 (65.85%), Query Frame = 3
Query: 4248 EIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQL 4493
E+ VF Y I+V+ RHL L AD++T +G++ FNR M++ SSPLQ+ +FET + + E L +G D + SP++ ++ G L
Sbjct: 2 EMNNVFAVYGIEVSKRHLSLTADYMTFTGQIQPFNRGAMSSSSSPLQKMTFETTMAFLREALLHGEEDNVNSPSARLVMGAL 83
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Fly
Match: RpI1 (gene:FBgn0019938 transcript:FBtr0087511) HSP 1 Score: 602.438 bits (1552), Expect = 0.000e+0 Identity = 434/1322 (32.83%), Postives = 655/1322 (49.55%), Query Frame = 3
Query: 96 FSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLND-KEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESI-CDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCH---------------NKSLVFKKSRKCEITVHISAKESRDKGEPSC-KRMKIDE--NPKIRDFKSSYSQEELYGKFTNT---DTSIFPEILREHLRLLWSNESENLQLILPMLKYNNF------KYPTDVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREIIK------RKDSTAAEQYYQNIR---------EGILELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVVENL-KFQLQN--NQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKL--------KLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETC-KNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVI----------------NSVSLSKPDFMEVIAKNIQLME----KRYLKEPRNTKPVLSKVLAKKIDELV---VKLKRDFVEISPK------DENLLREIIAVKSE--------YSICPN-------------------------------------GEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTL---------------GIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSV-LKKFTKLYLKDIIQLPFKITVGD----YYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKKT 3611
F+ +T +E+R LS ++ + I+ + LG GLYD +GS +PC C L DC GH G IE VYNP + + + C +C++L E I L I I + E KS+ + +N + I KN H N S K S + SA + K C K M+ + ++ + + +E G T + IF + R +LR +++N E L+L++P+L +N + P D+FFM+ + + PP R P M GD + +P+T N++ L ++K K + A+ YQ ++ L LQ V+V D RE+ + GL+Q I+KK GL RS++MGKRVN+A R+VI+PDP I +DEIG+P +F KL+YP P+ N L + V+NGPDV+PGA Q N F I + E+L K L N + I++RH L+GD L++NRQP+LH+PS+ H + +H KT R+HYSNCKAYNADFDGDEMN HYPQS +A+AEA LVNV + PKDG+PL GLIQDH+I+G K+ +R F R + LV+ GL + +++ KL P I+ P LW+GKQILS ++ N IP G E N+ W +S + LS+ ++ I +G + G+ DK G + GL + ELYG +V+ +LT +++ T +L GF+ +KD + + + R+ I+ + R++G+++V + + E ++ ME A + K KFR +D +K+L Y + + TC L KFP NNL LM +GAKG N +I+ LG IE+EGKR PL + G++LPSF ++ P S GF RFM+G++PQ+ FF MAGR+GL+DTAVKTS SGYLQRCLIKHLE ++V +D TVRD+ ++++QF YG DGLD++ N+ ++ +P ++++ QL + +++++ KP + E + V++KR EI+ K DE LL+ +E Y+ CP+ + + + +S+ P + L + G+ +TL GIPRLREILML S IKTPSMD+PI + L T L + + + +T+ Y + F F + YK ++ K +I F + + + K
Sbjct: 18 FAVFTDQEIRKLSVVKVITGITFD-ALGHAIPGGLYDIRMGSYGRCMDPCGTC-LKLQDCPGHMGHIELGTPVYNPFFIKFVQRLLCIFCLHCYKLQMKDHECEIIMLQLRLIDAGYIIEAQELELFKSEIVCQNTENLVAI------KNGDMVHPHIAAMYKLLEKNEKNSSNSTKTSCSLRTAITHSALQRLGKKCRHCNKSMRFVRYMHRRLVFYVTLADIKERVGTGAETGGQNKVIFADECRRYLRQIYANYPELLKLLVPVLGLSNTDLTQGDRSPVDLFFMDTLPVTPPRAR-PLNMVGDMLKGNPQTDIYINIIENNHVLNVVLKYMKGGQEKLTEEAKAAYQTLKGETAHEKLYTAWLALQMSVDVLLDVNMSREMKSGE-GLKQIIEKKSGLIRSHMMGKRVNYAARTVITPDPNINVDEIGIPDIFAKKLSYPVPVTEWNVTELRKMVMNGPDVHPGANYIQDKNGFTTYIPADNASKRESLAKLLLSNPKDGIKIVHRHVLNGDVLLLNRQPSLHKPSIMGHKARILHG--EKTFRLHYSNCKAYNADFDGDEMNAHYPQSEVARAEAYNLVNVASNYLVPKDGTPLGGLIQDHVISGVKLSIRGRFFNREDYQQLVFQGL-SQLKKDIKLLPPTILKPAVLWSGKQILSTIIINIIPEGYERINLDSFAKIAGKNWNVSRPRPPICGTNPEGNDLSESQVQIRNGELLVGVLDKQQYGATTYGLIHCMYELYGGDVSTLLLTAFTKVFTFFLQLEGFTLGVKDILVTDVADRKRRKIIRECRNVGNSAVAAALELE-DEPPHDELVEKME-AAYVKDSKFR---VLLDRKYKSLLDGYTNDINSTCLPRGLITKFPSNNLQLMVLSGAKGSMVNTMQISCLLGQIELEGKRPPLMISGKSLPSFTSFETSPKSGGFIDGRFMTGIQPQDFFFHCMAGREGLIDTAVKTSRSGYLQRCLIKHLEGLSVHYDLTVRDSDNSVVQFLYGEDGLDILKSKFFNDKFCADFLTQNATAILRPAQLQLMKDEEQLAKVQRHEKHIRSWEKKKPAKLRAAFTHFSEELREEVEVKRPN-EINSKTGRRRFDEGLLKLWKKADAEDKALYRKKYARCPDPTVAVYKQDLYYGSVSERTRKLITDYAKRKPALKETIADIMRVKTIKSLAAPGEPVGLIAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMLASSNIKTPSMDIPIKPGQQHQAEKLRINLNSVTLANLLEYVHVSTGLTLDPERSYEYDMRFQFLPREVYKEDYGVRPKHIIKYMHQTFFKQLIRAILKV 1320 HSP 2 Score: 134.035 bits (336), Expect = 6.281e-31 Identity = 71/186 (38.17%), Postives = 112/186 (60.22%), Query Frame = 3
Query: 3969 W--IKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIF-NSNKSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTNA 4517
W +KL L++ +++ D+ SI+ + V+ V +++A+I+ ++ + T+G N E+F+++ I DLN+ YSN+I ++R YGIEAA I E+ VFK Y I V+ RHL LIAD++T G +R M + SSPLQ+ SFE++L + +G D + SP+S +M G V N GT A
Sbjct: 1450 WCQVKLNLSVRYQKPDLTSIIRELAGKSVVHQVQHIKRAIIYKGTDDDQLLKTDGINIGEMFQHNKILDLNRLYSNDIHAIARTYGIEAASQVIVKEVSNVFKVYGITVDRRHLSLIADYMTFDGTFQPLSRKGMEHSSSPLQQMSFESSLQFLKSAAGFGRADELSSPSSRLMVGLPVRN-GTGA 1634
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Fly
Match: RpIIIC160 (gene:FBgn0030687 transcript:FBtr0300811) HSP 1 Score: 351.673 bits (901), Expect = 1.203e-99 Identity = 257/811 (31.69%), Postives = 404/811 (49.82%), Query Frame = 3
Query: 1134 GLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA-VSYQIGNRFPMVIGH-NPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQR-EFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKG-LCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLV----ETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNTKPVLSKV--------LAKKIDELVVKLKRDFVEISPK----------------------DENLLREIIAVKSEY--SICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDI 3446
G+ Q++ K G FR N+ GKRV+F+ R+VISPDP + ++++GVP+ LTYPE +NP N + + V NGP ++PGA Q G+ F + + N +V + LK ++ RH GD ++ NRQP+LH+ S+ H +A P+ +T R + C YNADFDGDEMN H PQ+ A+AEA +L+ TPK+G L QD I G + ++ T+ E + L L N + KLP PA++ P +LWTGKQ+ S++++ + + + L ++ K R + L +D + I + + G+ DK +G K + L +G A K + L+R+ + +L GFS I D K +L + + K E+ + K+ + + T +TL S + +L + K E P N+ L+M +G+KG N++++ +G I GKR P R LP F+R+ P +RGF + F SGL P E FF +MAGR+GLVDTAVKT+ +GYLQR L+K LED+ V +DGTVR+ ++ ++ YG DGLD ++ + +KP + N++ + P N + + + A+ D+ + ++ +S + E LL+ + + Y ++ G AVG +AAQS+GEP TQMTL TFH AG + MN+T G+PR+ EI+ + I TP + + C ++ A V + K L ++
Sbjct: 339 GIVQRLKGKQGRFRCNLSGKRVDFSGRTVISPDPNLMINQVGVPVRVAKILTYPERVNPANIRHMRELVRNGPSMHPGANYVQQRGSSFKKYLAYGNREKVAQELKC------GDVVERHLRDGDIVLFNRQPSLHKMSIMCH--RAKVQPQ-RTFRFNECACTPYNADFDGDEMNLHLPQTEEARAEALILMGNQSNLVTPKNGEILIAATQDFITGGYLLTQKEVFLTKEEAMQLAACFLANEDSTMHIKLPPPALLKPRRLWTGKQMFSLLMRPNDDSQVRLNMVN----KGRNYTR----NKDLCSNDSWIHIRNSELMCGVMDKATMGSGTKQCIFYLLLRDFGESHATKAMWRLARIASYFLQNRGFSFGISD-------VTPSKKLLQHKELLLNNGYAKC----------------NEYIELLKAGTLQCQPG--CTPEETLESVMLRELSAIREQAAKTCFAELHPTNSALIMALSGSKGSNINISQMIACVGQQAISGKRVPNGFENRALPHFERHSAIPAARGFVQNSFYSGLTPTEFFFHTMAGREGLVDTAVKTAETGYLQRRLVKCLEDLVVHYDGTVRNAVNEMVDTIYGGDGLDPVSMETRNKPVDLVHQYDNLRAQHPQGKDRPLNAEEMSEALETLLRTPEFAEARDDFKLDVRNHINTVSKRIGQLQKRYEKCIDLCHQIECLTTEQLLQFVRRINDRYNRAVTEPGTAVGAIAAQSIGEPGTQMTLKTFHFAGVASMNITQGVPRIVEIIN-ATKTISTPIITAELENC-HSMEFARQVKARIEKTTLAEL 1105 HSP 2 Score: 65.0846 bits (157), Expect = 6.374e-10 Identity = 44/165 (26.67%), Postives = 78/165 (47.27%), Query Frame = 3
Query: 4035 FENCVISSVAGVEKALIFNSNKSK----RFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTN-HSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTN 4514
+N V++ + + +A+I + + + EGY ++ + + SNNI ++ + GIEAA + I +EI V + + + V+ RH+ L+A +T G+V RH + S ASFE + + YG D I + I+ G + + IGT
Sbjct: 1189 LQNVVVAGLPNINRAVIAVDDARQPPTYKLCIEGYGLRDVIATYGVVG-KRTRSNNICEIYQTLGIEAARTIIMSEITEVMEGHGMSVDWRHIMLLASQMTARGEVLGITRHGLAKMRESVFNLASFEKTADHLFDAAYYGQTDAINGVSERIILG-MPACIGTG 1351
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Fly
Match: RpII215 (gene:FBgn0003277 transcript:FBtr0073542) HSP 1 Score: 317.005 bits (811), Expect = 1.772e-87 Identity = 232/713 (32.54%), Postives = 353/713 (49.51%), Query Frame = 3
Query: 846 KYPTDVFFMENMLINPPMNRLPAV-MNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDS---GRHRELTGS-QP--GLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPMVIGHNPLEVVENLKFQLQNNQTVI-----INRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEI--DTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGL 2936
KY + + +L PP+ PAV M G + T + ++++A LR K + S AA I+E I LQ V D+ G R + S +P ++ ++ K+G R N+MGKRV+F+ R+VI+PDP + +D++GVP LT+PE + P N D + + V G YPGA + G R +L+F +++ + + RH D +I NRQPTLH+ SM H +K + T RM+ S YNADFDGDEMN H PQS+ +AE + + Q TP+ P+ G++QD + A KM RD TR + ++L L + K+P+P I+ P LWTGKQI S++ IP + + + P + G + + D ++++ G + GI K LG S L G ++A + N+ ++ +L++ G S I D + + + K + + K+ N ME +L + F NK+ I D KT S K +L E NNL M +G+KG N++++ +G +EGKR P RTLP F + D P SRGF + +++GL P E +F +M GR+GL+DTAVKT+ +GY+QR LIK +E + V +DGTVR+++ +IQ RYG DGL
Sbjct: 233 KYARPDWMIVTVLPVPPLAVRPAVVMFGAAKNQDDLTHKLSDIIKANNELR---KNEASGAAAHV---IQENIKMLQFHVATLVDNDMPGMPRAMQKSGKPLKAIKARLKGKEGRIRGNLMGKRVDFSARTVITPDPNLRIDQVGVPRSIAQNLTFPELVTPFNIDRMQELVRRGNSQYPGAKYIVRDNGERI-------------DLRFHPKSSDLHLQCGYKVERHLRDDDLVIFNRQPTLHKMSMMGHRVKVL---PWSTFRMNLSCTSPYNADFDGDEMNLHVPQSMETRAEVENIHITPRQIITPQANKPVMGIVQDTLTAVRKMTKRDVFITREQVMNL----LMFLPTWDAKMPQPCILKPRPLWTGKQIFSLI----IPGNVNMIRTHSTHPDEED----EGPYKWISPGDTKVMVEHGELIMGILCKKSLGTSAGSLLHICFLELGHDIAGRFYGNIQTVINNWLLFEGHSIGIGDTIADPQTYNEIQQAIKKAKDDVINVIQKAHN---MELEPTPGNTLRQ--------TFENKVNRILNDARDKTGGS---------AKKSLTEY---NNLKAMVVSGSKGSNINISQVIACVGQQNVEGKRIPYGFRKRTLPHFIKDDYGPESRGFVENSYLAGLTPSEFYFHAMGGREGLIDTAVKTAETGYIQRRLIKAMESVMVNYDGTVRNSVGQLIQLRYGEDGL 888 HSP 2 Score: 78.9518 bits (193), Expect = 3.809e-14 Identity = 40/59 (67.80%), Postives = 47/59 (79.66%), Query Frame = 3
Query: 3189 GEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDV 3365
GE VG LAAQS+GEP+TQMTLNTFH AG S NVTLG+PRL+EI+ + S K K PS+ V
Sbjct: 1076 GEMVGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINI-SKKPKAPSLTV 1133 HSP 3 Score: 68.9366 bits (167), Expect = 4.165e-11 Identity = 38/127 (29.92%), Postives = 68/127 (53.54%), Query Frame = 3
Query: 4116 TEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNH-SSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQL 4493
T+G + +++ + D + SN+I ++ ++ GIEA +E E+ V + Y + VN RHL L+ D +T G + A RH + + L R SFE + V+++ + D ++ + NI+ GQL
Sbjct: 1330 TDGTSMMKVLSERDV-DPIRTSSNDICEIFQVLGIEAVRKSVEKEMNAVLQFYGLYVNYRHLALLCDVMTAKGHLMAITRHGINRQDTGALMRCSFEETVDVLMDAAAHAETDPMRGVSENIIMGQL 1455 HSP 4 Score: 60.4622 bits (145), Expect = 1.681e-8 Identity = 27/97 (27.84%), Postives = 48/97 (49.48%), Query Frame = 3
Query: 90 ICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRL 380
+ F +P+E+R +S E + G+ + GL D G ++ C+ C + +C GHFG I+ +K V++ K ++ VCFYC ++
Sbjct: 17 VQFGILSPDEIRRMSVTEGGVQFAETMEGGRPKLGGLMDPRQGVIDRTSRCQTCAGNMTECPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYCSKM 113
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Zebrafish
Match: polr1a (RNA polymerase I subunit A [Source:ZFIN;Acc:ZDB-GENE-030131-5286]) HSP 1 Score: 582.793 bits (1501), Expect = 9.340e-180 Identity = 383/1056 (36.27%), Postives = 561/1056 (53.12%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKD-SLIKRFNDYENMKSK---------------TLSDKQKNALKIMSSQTRKNC--HQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCK--RMKI--DENPKIRDFKSSYSQEELYGKFTNTDTSIF--PEILREHLRLLWSNESENLQLILPMLKYNNFK--YPTDVFFMENMLINP----PMNRLPAVMNGDQMTESPETTNMCNVVR----------------------AEAALREIIKRKDSTAAEQYYQNIREGI----------LELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPMVIGHNPLEVVENLKFQLQNNQT-------VIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW-----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYL-VYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKN-NLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEK 3014
L+ + F ++ +E R LS K IT+ ++ V G A NGLYD +LG ++KE C C F C GHFG IE VYNPL FD L I+ C CH L + + + ++ D +K + EN+ ++ TL+D K L+ + + H C KS + + +H+ K+ ++ CK RM + + N K+ ++ + + + IF P + REH+ LW E L+ + +N + +VFF+E +++ P P+NRL GDQM + +T NM V++ + L + + T +Q + +G+ + LQ VN+ +DS + +T PG+RQ ++KK+GLFR ++MGKRV++A RSVI PD IG +EIG+P+VF +KLTYP+P+ P N + Q VLNGP+++PGA V + G+R ++ E + QL T I+NRH +GD L++NRQPTLHRPS+QAH + + P K LR+HY+NCKAYNADFDGDEMN H+PQS L++AEA LV+ Q+ PKDG PLAGLIQDH+++GT M +R FTR++ LVY GL + R KL P +I P LWTGKQ++S +L N IP N++ W R + + +++I G + G+ DK H G S GL ELYG E + K+L+ L+RL T YL +Y GF+ ++D + + RK I+ K + GS ++ +FN+P + T E Q + + +D FK + + + + C L FP+NNL +M Q+GAKG N +I+ LG IE+EG+R PL G++LP F+ Y+ P S GF RF++G+KP E FF MAGR+GLVDTAVKTS SGYLQRC+IKHLE + V +D TVRD+ +++QF YG DGLD+ + L +P I N +++ K
Sbjct: 12 LEGMSFGLFSADETRKLSVKTITNPQLLDNV-GNAAANGLYDLALGPADNKEICSTCMQDFSSCPGHFGHIELPLPVYNPLFFDKLYLLIRGSCLSCHILKCPRAAIHLLLCQLKLLDVGAMKEVFEIENVLNQFLESNPSPPGEEIQETLNDFVKPILETRKDKQHTDPVKHICEKKSSLITSFWR----IHMGPKKCQN-----CKANRMTVRKEHNSKLIVTQTLHKKASEQDDDSQLTKRIFLTPSVAREHIIKLWETEGFFLKHLFSGFDMDNSDNGFNPEVFFLELLVVPPCRYRPINRL-----GDQMFTNGQTVNMQAVLKDGLIIQKLLTLIADEKTKAIEGTQTELEQSSEAPKVTEIDQSFLTSIQGVTLTDKLYSTWIHLQSLVNIVFDSDMDKLITEKFPGVRQLLEKKEGLFRKHMMGKRVDYAARSVICPDMYIGTNEIGIPMVFATKLTYPQPVTPWNVKEMRQAVLNGPNIHPGATMVVNEDGSR--TILSSTNQTQREAVAKQLLTPSTGQHRIPMKIVNRHIKNGDVLLLNRQPTLHRPSIQAHCARIL--PGEKVLRLHYANCKAYNADFDGDEMNAHFPQSELSRAEAYTLVSTDQQYLVPKDGKPLAGLIQDHMVSGTSMTIRGCFFTRDQYTALVYRGLTDKIGR-VKLLPPTLIKPVLLWTGKQVVSTLLLNVIPENSIPLNLTGKAKIPGKAWVKESPRPVPGYVPESMCESQVVIRQGELLMGVLDKAHYGSSAYGLVHCCYELYGGETSGKLLSCLARLFTAYLQLYRGFTLGVEDILVKDGANKQRKKIIKKSVTCGSKALQAAFNLPA----SAEDTEARERWQDAHLNIDQRDFNMVDLKFKEEVNQVNNNINKVCMPLGLHRSFPENNLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMPSGKSLPCFQPYEPQPRSGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCVIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFL-QPRQFPFIKDNYEVIRK 1042 HSP 2 Score: 232.646 bits (592), Expect = 3.998e-61 Identity = 156/500 (31.20%), Postives = 263/500 (52.60%), Query Frame = 3
Query: 3129 DENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDII---------QLPFKITV-GDYYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKK-TFKQTLINITDNK------------------------DEDNQAVQTDLVNEDELNNPETEANNPEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNAAFMDEEQSEYISKTKANVKAM--LPGVFHNYVIESKPEPDW--IKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALI-----FNSNKSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
D + LR ++ +K + ++C GEAVGL+AAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPRLREIL++ S IKTP M +P++ A+ ++ KK T++ L +++ ++P K T+ + ITF F Y+ L E++ E F + + +KK T K I++ + + D +N+ + D NE + + +++ +++ D + + ++++ EE+E E +E E+ ++ EQ + S+ + ++ L +Y ++K + W + L + + FD+ S+++ +N VI G+ + L+ + +K TEG N E+FKY I DL + YSN + M+ YGIE A+ IE EIK VF Y I+V+ RHL L+AD++ G NR + ++SSPLQ+ +FET+ + + G+ D + SP++ ++ G++V
Sbjct: 1181 DADRLRNLLQLKWQRALCDPGEAVGLVAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILLVASSNIKTPMMSIPVVMNKKALKQVKTLRKKITRVCLSEVLHKVNVIESFRMPKKETLKSRVFKITFHFLPPERYQQDKLLNPDEILHYMEQTFFRMLLEAIKKLTAKLAAISLVETRRATVNDNDRDTSDGGADREDNGGEDAENERIVDDEANEGDADATDSKRKEKQEEEVDYESEEGEEGSDQEQEEVAEEQEASQEVSEEGSTESQQRVNSEQPKGSSQGSVRINSVLQLSAAIEDYKYDTK-KGLWCELTLVMPITKVHFDLTSVVVKQAQNAVIMETKGITRCLLNEVTRKDGSKEIMLNTEGINIHEMFKYADILDLKRLYSNEVHAMASTYGIEVALRVIEKEIKDVFAVYGIEVDPRHLSLVADYMCFEGVYKPLNRFGIQSNSSPLQQMTFETSFKFLKQATMLGSYDKLSSPSACLVVGKVV 1679
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Zebrafish
Match: polr1a (RNA polymerase I subunit A [Source:ZFIN;Acc:ZDB-GENE-030131-5286]) HSP 1 Score: 580.096 bits (1494), Expect = 8.444e-179 Identity = 381/1054 (36.15%), Postives = 560/1054 (53.13%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKD-SLIKRFNDYENMKSK---------------TLSDKQKNALKIMSSQTRKNC--HQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCK--RMKI--DENPKIRDFKSSYSQEELYGKFTNTDTSIF--PEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINP----PMNRLPAVMNGDQMTESPETTNMCNVVR----------------------AEAALREIIKRKDSTAAEQYYQNIREGI----------LELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPMVIGHNPLEVVENLKFQLQNNQT-------VIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW-----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYL-VYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKN-NLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEK 3014
L+ + F ++ +E R LS K IT+ ++ V G A NGLYD +LG ++KE C C F C GHFG IE VYNPL FD L I+ C CH L + + + ++ D +K + EN+ ++ TL+D K L+ + + H C KS + + +H+ K+ ++ CK RM + + N K+ ++ + + + IF P + REH+ LW E ++ ++ + +VFF+E +++ P P+NRL GDQM + +T NM V++ + L + + T +Q + +G+ + LQ VN+ +DS + +T PG+RQ ++KK+GLFR ++MGKRV++A RSVI PD IG +EIG+P+VF +KLTYP+P+ P N + Q VLNGP+++PGA V + G+R ++ E + QL T I+NRH +GD L++NRQPTLHRPS+QAH + + P K LR+HY+NCKAYNADFDGDEMN H+PQS L++AEA LV+ Q+ PKDG PLAGLIQDH+++GT M +R FTR++ LVY GL + R KL P +I P LWTGKQ++S +L N IP N++ W R + + +++I G + G+ DK H G S GL ELYG E + K+L+ L+RL T YL +Y GF+ ++D + + RK I+ K + GS ++ +FN+P + T E Q + + +D FK + + + + C L FP+NNL +M Q+GAKG N +I+ LG IE+EG+R PL G++LP F+ Y+ P S GF RF++G+KP E FF MAGR+GLVDTAVKTS SGYLQRC+IKHLE + V +D TVRD+ +++QF YG DGLD+ + L +P I N +++ K
Sbjct: 12 LEGMSFGLFSADETRKLSVKTITNPQLLDNV-GNAAANGLYDLALGPADNKEICSTCMQDFSSCPGHFGHIELPLPVYNPLFFDKLYLLIRGSCLSCHILKCPRAAIHLLLCQLKLLDVGAMKEVFEIENVLNQFLESNPSPPGEEIQETLNDFVKPILETRKDKQHTDPVKHICEKKSSLITSFWR----IHMGPKKCQN-----CKANRMTVRKEHNSKLIVTQTLHKKASEQDDDSQLTKRIFLTPSVAREHIIKLWETEGFDMD-------NSDNGFNPEVFFLELLVVPPCRYRPINRL-----GDQMFTNGQTVNMQAVLKDGLIIQKLLTLIADEKTKAIEGTQTELEQSSEAPKVTEIDQSFLTSIQGVTLTDKLYSTWIHLQSLVNIVFDSDMDKLITEKFPGVRQLLEKKEGLFRKHMMGKRVDYAARSVICPDMYIGTNEIGIPMVFATKLTYPQPVTPWNVKEMRQAVLNGPNIHPGATMVVNEDGSR--TILSSTNQTQREAVAKQLLTPSTGQHRIPMKIVNRHIKNGDVLLLNRQPTLHRPSIQAHCARIL--PGEKVLRLHYANCKAYNADFDGDEMNAHFPQSELSRAEAYTLVSTDQQYLVPKDGKPLAGLIQDHMVSGTSMTIRGCFFTRDQYTALVYRGLTDKIGR-VKLLPPTLIKPVLLWTGKQVVSTLLLNVIPENSIPLNLTGKAKIPGKAWVKESPRPVPGYVPESMCESQVVIRQGELLMGVLDKAHYGSSAYGLVHCCYELYGGETSGKLLSCLARLFTAYLQLYRGFTLGVEDILVKDGANKQRKKIIKKSVTCGSKALQAAFNLPA----SAEDTEARERWQDAHLNIDQRDFNMVDLKFKEEVNQVNNNINKVCMPLGLHRSFPENNLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMPSGKSLPCFQPYEPQPRSGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCVIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFL-QPRQFPFIKDNYEVIRK 1033 HSP 2 Score: 232.646 bits (592), Expect = 4.103e-61 Identity = 156/500 (31.20%), Postives = 263/500 (52.60%), Query Frame = 3
Query: 3129 DENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDII---------QLPFKITV-GDYYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKK-TFKQTLINITDNK------------------------DEDNQAVQTDLVNEDELNNPETEANNPEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNAAFMDEEQSEYISKTKANVKAM--LPGVFHNYVIESKPEPDW--IKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALI-----FNSNKSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
D + LR ++ +K + ++C GEAVGL+AAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPRLREIL++ S IKTP M +P++ A+ ++ KK T++ L +++ ++P K T+ + ITF F Y+ L E++ E F + + +KK T K I++ + + D +N+ + D NE + + +++ +++ D + + ++++ EE+E E +E E+ ++ EQ + S+ + ++ L +Y ++K + W + L + + FD+ S+++ +N VI G+ + L+ + +K TEG N E+FKY I DL + YSN + M+ YGIE A+ IE EIK VF Y I+V+ RHL L+AD++ G NR + ++SSPLQ+ +FET+ + + G+ D + SP++ ++ G++V
Sbjct: 1172 DADRLRNLLQLKWQRALCDPGEAVGLVAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILLVASSNIKTPMMSIPVVMNKKALKQVKTLRKKITRVCLSEVLHKVNVIESFRMPKKETLKSRVFKITFHFLPPERYQQDKLLNPDEILHYMEQTFFRMLLEAIKKLTAKLAAISLVETRRATVNDNDRDTSDGGADREDNGGEDAENERIVDDEANEGDADATDSKRKEKQEEEVDYESEEGEEGSDQEQEEVAEEQEASQEVSEEGSTESQQRVNSEQPKGSSQGSVRINSVLQLSAAIEDYKYDTK-KGLWCELTLVMPITKVHFDLTSVVVKQAQNAVIMETKGITRCLLNEVTRKDGSKEIMLNTEGINIHEMFKYADILDLKRLYSNEVHAMASTYGIEVALRVIEKEIKDVFAVYGIEVDPRHLSLVADYMCFEGVYKPLNRFGIQSNSSPLQQMTFETSFKFLKQATMLGSYDKLSSPSACLVVGKVV 1670
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Zebrafish
Match: polr3a (polymerase (RNA) III (DNA directed) polypeptide A [Source:ZFIN;Acc:ZDB-GENE-050208-103]) HSP 1 Score: 388.267 bits (996), Expect = 5.203e-112 Identity = 339/1184 (28.63%), Postives = 531/1184 (44.85%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSL-ISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYS-----QEELYGKFTNTDTSIFPEILREHLRLLWSNESENLQLILPMLKYN-NFKYPTDVFFMENMLINPPMNRLPAVMNG--DQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDSGRHRELTG---------SQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQ-----------IGNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQR-EFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPK--KNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQK-GLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKF-------RNKMTEIDTNFKTLSSNYIHQLV----ETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQ----------------------LMEKR--------YLKEPRNTKPVLSKVLAKKIDELVV--------KLKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDD 3386
+ ICF + E++R + ++ S + + K G+ D +G+ PC C + DC GH+G ++ ++ F + ++ +C C + T+ ++ L +F DY ++ L+ QK LK S + C N + +KC + I K K K +P + DF S+ +E+ T ++ P ++ R + + +P+L N P D+ L+ PP+ P+V++ E T + ++ L ++IK+ T A+ Q I E LQ + + +S EL+G G Q++ K G FR N+ GKRV+F+ R+VISPDP + +DE+ VP+ LTYPE +N N + + + V NGP+V+PGA Q GNR M + LKF ++ RH + GD ++ NRQP+LH+ S+ AHI + +T R + C YNADFDGDEMN H PQ+ A+AEA +L+ TP++G PL IQD + + L+D+ F R + +V L +++ + LP PAI+ P +LWTGKQI S++LK P+ SC + + G + L +D ++I + + G DK LG K + L +G + A ++ L+RL YL GFS I D + A++ +L+ A ++K D++ R + T +TL + + +L LRE N+ L+M G+KG N++++ +G I G R P R+LP F+++ P ++GF F SGL P E FF +MAGR+GLVDTAVKT+ +GY+QR L+K LED+ +D TVR + +IIQF YG DGLD +P + + NI+ +M+++ +L+E R +S+ + K D+ + K+ I+P E K + G AVG L AQS+GEP TQMTL TFH AG + MN+TLG+PR++EI+ S I TP + + DD
Sbjct: 15 ISHICFGMKSSEQMRQQAHIQVVSKNLYSQDTSHKPLQYGVLDHRMGTSEKDRPCETCGKNLADCLGHYGYLDLELPCFHIGYFKAIVGILQMICKTCSHILLTK------------EEKL--QFLDY--LRRPGLAYLQKRGLKKKISDKCRKKTVCLNCNAFNGSVKKCGLLKIIHEKY---------KSTKKVVDPAVSDFLQSFDVAIEHNKEVESLLTRAQENLNPLVVLNLFRRIPDKD-------VPLLLMNPESGKPADLILTR--LLVPPLCIRPSVVSDLKSGTNEDDLTMKLTEII----FLNDVIKKHRMTGAKT--QMIMEDWDFLQLQCALYINS----ELSGIPLNMAPKKWTRGFVQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLRIDEVAVPVHVAKILTYPEKVNKANIELMRKLVRNGPEVHPGANFIQQRHMQMKRFLKYGNREKM---------AQELKF------GDVVERHMIDGDIVLFNRQPSLHKLSIMAHIARV---KPHRTFRFNECVCTPYNADFDGDEMNLHLPQTEEAKAEALVLMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTFFDRTKACQIVASILVGKDEKIKISLPHPAILKPIRLWTGKQIFSLILK---------PSKSCPVMANLRTKGKQYCGKGEDLCANDSFVVIHNSELMCGSMDKGTLGSGSKNNIFYILLRDWGQQEAADAMSRLARLAPVYLSNRGFSIGIGDVTPGQGLLKAKQELLD--------------------------------AGYEKCDEYIDALKTGRLQQQPGCTAEETLEALILKELSVIRDHAGSACLRELDKSNSPLIMALCGSKGSFINISQMIACVGQQAISGSRVPDGFENRSLPHFQKHSKLPAAKGFVADSFYSGLTPTEFFFHTMAGREGLVDTAVKTAETGYMQRRLVKSLEDLCSQYDLTVRSSTGDIIQFIYGGDGLDPAAMEGKDEPLEFKRVLDNIRAVYSCTGEPALSRNELLLTSDSIMKRKDFLCCKDTFLEEIRKFIKTVSEKVKKTRDKYGINDNGTTEPKVLYQLDRITPTQLEKFLETCRDKYMRAQMEPGSAVGALCAQSIGEPGTQMTLKTFHFAGVASMNITLGVPRIKEIIN-ASKNISTPIISANLDTNDD 1094 HSP 2 Score: 60.077 bits (144), Expect = 2.419e-8 Identity = 45/161 (27.95%), Postives = 78/161 (48.45%), Query Frame = 3
Query: 4047 VISSVAGVEKALIF---NSNKSK-RFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTN-HSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTN 4514
V+ + V +A+I S K K + EG N + + + +K SNN ++ + GIEAA S I NEI+ ++ + ++ RH+ L+AD +++ G++ R + S L ASFE + + +G D + + I+ G + NIGT
Sbjct: 1200 VVQGIPEVSRAVIHIDEQSGKQKFKLLVEGDNMRSVMATHGV-NGSKTTSNNTYEVEKTLGIEAARSTIINEIQYTMVNHGMSIDRRHVMLLADLMSYKGEILGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMG-IPMNIGTG 1358
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Zebrafish
Match: polr2a (polymerase (RNA) II (DNA directed) polypeptide A [Source:ZFIN;Acc:ZDB-GENE-041008-78]) HSP 1 Score: 362.459 bits (929), Expect = 8.551e-102 Identity = 311/1083 (28.72%), Postives = 496/1083 (45.80%), Query Frame = 3
Query: 90 ICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQ----CHNKSLVFKKSRKCEITVHISAKESRD----KGEPSCKRMKIDENPKIRDFKSSYSQEELYG--KFTNTDTS-----IFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPA-VMNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDS---GRHRELTGS-QP--GLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQI---GNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEI--DTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNTKPVLSKVLAKKIDELVVK-----------LKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSV 3224
+ F +P+E++ +S E G+ + GL D G + C+ C + +C GHFG IE +K V++ + K ++ VCF+C +L D N K K + K K Q RK C K++ + + + + ES D KG C R + P+IR S ELY K N D+ + PE + E + + E +IL M + KY + + +L PP+ PA VM G + T + ++V+ + +KR + + A + I E + LQ V D+ G R + S +P ++Q++ K+G R N+MGKRV+F+ R+VI+PDP + +D++GVP + +T+PE + P N D L + V G YPGA Y I G+R + P ++ + ++++ RH GD ++ NRQPTLH+ SM H ++ + T R++ S YNADFDGDEMN H PQSL +AE + L V TP+ P+ G++QD + A K RD R E ++L L + + K+P+PAI+ P LWTGKQI S++ IP + + P + SG + + D ++I+ +G + GI K LG S L G ++ +N+ ++ +L+ G S I D +++ +N + K + + K+ N E +L + F N++ I D KT SS + +L E NN M G+KG + N++++ +G +EGKR P RTLP F + D P SRGF + +++GL P E FF +M GR+GL+DTAVKT+ +GY+QR LIK +E + V +D TVR++++ ++Q RYG DGL +V M + + + EK++ + N + L + + E VVK L+R+F ++ +D +LR I + P A + AQ +
Sbjct: 19 VQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERSGRCQTCAGNMTECPGHFGHIELAKPVFHVGFITKIMKVLRCVCFFCSKL-----------------------LVDANNPKIKDILTKSKG-------QPRKRLTHVYDLCKGKNIC-EGGEEMDNKFGVEQPESEDITKEKGHGGCGRYQ----PRIR-----RSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRIADEE----DIILGM----DPKYARPEWMIVTVLPVPPLAVRPAVVMQGSARNQDDLTHKLADIVK----INNQLKRNEQSGAAAHV--IAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSIKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLQIDQVGVPRSIAANMTFPEIVTPFNIDRLQELVRRGNSQYPGA-KYIIRDNGDRIDLRFHPKPSDLHLQIGYKVE--------RHMCDGDIIVFNRQPTLHKMSMMGHRVRIL---PWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNL----LMFLSTWDGKVPQPAILKPRPLWTGKQIFSLI----IPGHINAIRTHSTHPDEED----SGPYKNISPGDTKVIVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVVNNWLLIEGHSIGIGDSIADKATYQDIQNTIKKAKQDVIEVIEKAHN---NELEPTPGNTLRQ--------TFENQVNRILNDARDKTGSS---------AQKSLSEY---NNFKSMVVAGSKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGL-AGEAVEFQN---MATLKPSHKAFEKKFKFDYAN-----ERALRRTLQEDVVKDVMTNAHVQSSLEREFEKMR-EDREILRAIFPTGDSKVVLPCNLARMIWNAQKI 990 HSP 2 Score: 82.0333 bits (201), Expect = 5.649e-15 Identity = 47/81 (58.02%), Postives = 57/81 (70.37%), Query Frame = 3
Query: 3153 IAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPIL--ACDDA 3389
I K SI GE VG LAAQS+GEP+TQMTLNTFH AG S NVTLG+PRL+E++ + S + KTPS+ V +L A DA
Sbjct: 1069 IETKFNQSIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINI-SKRPKTPSLTVFLLGQAARDA 1148 HSP 3 Score: 68.5514 bits (166), Expect = 6.876e-11 Identity = 41/128 (32.03%), Postives = 67/128 (52.34%), Query Frame = 3
Query: 4116 TEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNH-SSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
T+G + + + + D + SN+I+++ + GIEA +E E+ V VN RHL L+ D +T G + A RH + + PL + SFE + V++E ++G D +K + NIM GQL
Sbjct: 1335 TDGVSLMRVLSEKDV-DPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGINRQDTGPLMKCSFEETVDVLMEASSHGESDPMKGVSENIMLGQLA 1461
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Xenopus
Match: POLR1A (polymerase (RNA) I polypeptide A [Source:NCBI gene;Acc:100158635]) HSP 1 Score: 549.666 bits (1415), Expect = 3.278e-168 Identity = 379/1107 (34.24%), Postives = 566/1107 (51.13%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKD-SLIKRFNDYENMKSKTLSDKQKNALKI-------MSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYGKFTNTDTSIF----------PEILREHLRLLWSNESENLQLILPMLKYNN-FKYPTDVFFMENMLINP----PMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREII--------KRKDSTAAEQYYQNIREGIL-----------------ELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPMVIGHNPLEVVENLKFQL-------QNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW-----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYL-VYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKN-NLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNTKPVLSKV---LAKKIDELVVKLKRDFVEISPKDENLL----REIIAVKSEYSICPN 3188
L + F Y+ EE+R LS K +T+ ++ LG +NGLYD +LG + KE C C F +C GH G IE VYNPL FD L ++ CF+CH L + ++ + + ++ D + ++ EN+ + L + NA + TRK V KK +K G S K + N I +F + + + K N ++++ P +E ++ LW NE LQ + + N + D FF++ +++ P P+NRL GDQ+ + T N+ V++ + +++++ + + T + +NI L LQ VN+ +DS + +T PG+RQ ++KK+GLFR ++MGKRV++A RSVI PD I +EIG+P+VF +KLTYP+P+ P N L Q V+NGP+V+PGA V + G+R ++ + + E + QL Q I+ RH +GD L++NRQPTLHRPS+QAH + + P K LR+HY+NCKAYNADFDGDEMN H+PQS L +AEA L Q+ PKDG+PLAGLIQDH+++G M +R FTR + ++LVY GL + R LP PAI+ P LWTGKQ++S +L N IP L N++ W R + + ++II DG + G+ DK H G S GL ELYG E + K+LT L+RL T YL +Y GF+ ++D + + R NI+ + G ++ +FN+ E+A + A K + N +D FK + + +++ + C L FP+NNL LM Q+GAKG N + + + G LPS C+ +F+ L QE FF MAGR+GLVDTAVKTS SGYLQRC+IKHLE + V +D TVRD+ ++IQF YG DGLDV + L +P IA+N ++ +K+ ++ VLS+V A++ + + + K +S + L +++ A K+ CPN
Sbjct: 12 LQGVSFGCYSAEEIRKLSVKALTNPHYMDN-LGNPMMNGLYDLALGPADGKEVCSTCAQDFNNCPGHLGHIELPLTVYNPLFFDKLYLLVRGSCFHCHLLTCHRSVIHLLLNQLKVLDRGAVHEVHELENVLHRLL---EANAAATGNEIREELGDHTRK----------VLKKYKK-------------QPGGISVKNTCVTRNQLIGNF---WKTQMVSAKCPNCNSTLTLPLAKRGYLTPNSTKELIKALWENEGFFLQHLFSGITGNGESAFTPDTFFLDIIVVPPSRYRPVNRL-----GDQVFTNGHTVNLQAVLKDVSMIQKLLAVMAQDQEQMPEVTESGMITENIDRSFLATLMGQSLTDKLYNSWVRLQSHVNIVFDSDMDKLMTEKYPGIRQVLEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVMNGPNVHPGASMVISEDGSR--TILNSSNVSQREAVAKQLLTPSTGAQTLAAKIVCRHVKNGDVLLLNRQPTLHRPSIQAHKARIL--PGEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYSLACTDEQYLVPKDGTPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYQGLTDKRGRVKVLP-PAILKPYPLWTGKQVVSTLLINVIPENLIPLNLTGKAKISGKAWVKEQPRPIPGFSPDSMCESQVIIRDGELLCGVLDKAHYGSSSFGLVHCCYELYGGETSGKLLTCLARLFTAYLQLYRGFTLGVEDILVLPYADAQRLNIVQQSLHCGGKAIRAAFNLSEAA-SDEEAHGKWQDAHLSKDQRDFNM---VDLKFKEEVNQFNNEINKVCMPFGLHRPFPENNLQLMVQSGAKGSTVNTMQNISF------------MPIPGALLPS-----------NLCLYQFVISL--QEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVIQFLYGEDGLDVPKTQFL-QPKQFPFIAENYEV-----IKQSKHLDEVLSRVNTEQAEQHNHAIYRWKARHPNLSQRISPFLLYSQKQMAAAKAS---CPN 1040 HSP 2 Score: 121.709 bits (304), Expect = 5.952e-27 Identity = 62/180 (34.44%), Postives = 106/180 (58.89%), Query Frame = 3
Query: 3972 IKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNS-----NKSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
I + L L FD+ S++ ++ + ++ +G+ + L+ ++ +K TEG N ELFKY +I +L + YSN+I M++ +GIEAA+ +E EIK VF Y I+V+ RHL L+AD++ G NR M + SSP Q+ +FET+ + + G D ++SP++ ++ G++V
Sbjct: 1390 ITIKLQLMKIHFDICSLVSSLAHSAIVHETSGITRCLLNDTANKQGDKELILNTEGINLYELFKYSNILELRRLYSNDIHTMAKTFGIEAALRVVEREIKDVFAVYGIEVDPRHLSLVADYMCFEGTYKPLNRIGMQSSSSPFQQMTFETSYKFLKDATMNGARDDLQSPSACLVVGKVV 1569 HSP 3 Score: 115.546 bits (288), Expect = 4.720e-25 Identity = 59/104 (56.73%), Postives = 77/104 (74.04%), Query Frame = 3
Query: 3141 LREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQ 3452
L+ ++ +K + ++C G+AVGLLAAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPRLREILM+ S IKTP M VP+L A+ + KK T++ L ++I
Sbjct: 1126 LKTLLHLKWQRALCDPGDAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKAVKKVKQLKKKLTRVCLAEVIH 1229
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Xenopus
Match: POLR1A (polymerase (RNA) I polypeptide A [Source:NCBI gene;Acc:100158635]) HSP 1 Score: 547.354 bits (1409), Expect = 5.396e-167 Identity = 379/1104 (34.33%), Postives = 565/1104 (51.18%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKD-SLIKRFNDYENMKSKTLSDKQKNALKI-------MSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYGKFTNTDTSIF----------PEILREHLRLLWSNESENLQLILPMLKYNN-FKYPTDVFFMENMLINP----PMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGI-------------------------LELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPMVIGHNPLEVVENLKFQL----QNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW-----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYL-VYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKN-NLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNTKPVLSKV---LAKKIDELVVKLKRDFVEISPKDENLL----REIIAVKSEYSICPN 3188
L + F Y+ EE+R LS K +T+ ++ LG +NGLYD +LG + KE C C F +C GH G IE VYNPL FD L ++ CF+CH L + ++ + + ++ D + ++ EN+ + L + NA + TRK V KK +K G S K + N I +F + + + K N ++++ P +E ++ LW NE LQ + + N + D FF++ +++ P P+NRL GDQ+ + T N+ V++ + +++++ + EQ + G+ + LQ VN+ +DS + +T PG+RQ ++KK+GLFR ++MGKRV++A RSVI PD I +EIG+P+VF +KLTYP+P+ P N L Q V+NGP+V+PGA V + G+R ++ + + E + QL QT+ + RH +GD L++NRQPTLHRPS+QAH + + P K LR+HY+NCKAYNADFDGDEMN H+PQS L +AEA L Q+ PKDG+PLAGLIQDH+++G M +R FTR + ++LVY GL + R LP PAI+ P LWTGKQ++S +L N IP L N++ W R + + ++II DG + G+ DK H G S GL ELYG E + K+LT L+RL T YL +Y GF+ ++D + + R NI+ + G ++ +FN+ E+A + A K + N +D FK + + +++ + C L FP+NNL LM Q+GAKG N G PL G+ L DL +R+ + QE FF MAGR+GLVDTAVKTS SGYLQRC+IKHLE + V +D TVRD+ ++IQF YG DGLDV + L +P IA+N ++ +K+ ++ VLS+V A++ + + + K +S + L +++ A K+ CPN
Sbjct: 12 LQGVSFGCYSAEEIRKLSVKALTNPHYMDN-LGNPMMNGLYDLALGPADGKEVCSTCAQDFNNCPGHLGHIELPLTVYNPLFFDKLYLLVRGSCFHCHLLTCHRSVIHLLLNQLKVLDRGAVHEVHELENVLHRLL---EANAAATGNEIREELGDHTRK----------VLKKYKK-------------QPGGISVKNTCVTRNQLIGNF---WKTQMVSAKCPNCNSTLTLPLAKRGYLTPNSTKELIKALWENEGFFLQHLFSGITGNGESAFTPDTFFLDIIVVPPSRYRPVNRL-----GDQVFTNGHTVNLQAVLKDVSMIQKLLAVM-AQDQEQMPEVTESGMSLNIDRSFLATLMGQSLTDKLYNSWVRLQSHVNIVFDSDMDKLMTEKYPGIRQVLEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVMNGPNVHPGASMVISEDGSR--TILNSSNVSQREAVAKQLLTPSTGAQTLAVCRHVKNGDVLLLNRQPTLHRPSIQAHKARIL--PGEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYSLACTDEQYLVPKDGTPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYQGLTDKRGRVKVLP-PAILKPYPLWTGKQVVSTLLINVIPENLIPLNLTGKAKISGKAWVKEQPRPIPGFSPDSMCESQVIIRDGELLCGVLDKAHYGSSSFGLVHCCYELYGGETSGKLLTCLARLFTAYLQLYRGFTLGVEDILVLPYADAQRLNIVQQSLHCGGKAIRAAFNLSEAA-SDEEAHGKWQDAHLSKDQRDFNM---VDLKFKEEVNQFNNEINKVCMPFGLHRPFPENNLQLMVQSGAKGSTVNTI-------FTGGGGYSSPLQSTGKCLRYCGFSDL--------TNRYQFVISLQEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVIQFLYGEDGLDVPKTQFL-QPKQFPFIAENYEV-----IKQSKHLDEVLSRVNTEQAEQHNHAIYRWKARHPNLSQRISPFLLYSQKQMAAAKAS---CPN 1046 HSP 2 Score: 121.709 bits (304), Expect = 5.860e-27 Identity = 62/180 (34.44%), Postives = 106/180 (58.89%), Query Frame = 3
Query: 3972 IKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNS-----NKSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
I + L L FD+ S++ ++ + ++ +G+ + L+ ++ +K TEG N ELFKY +I +L + YSN+I M++ +GIEAA+ +E EIK VF Y I+V+ RHL L+AD++ G NR M + SSP Q+ +FET+ + + G D ++SP++ ++ G++V
Sbjct: 1449 ITIKLQLMKIHFDICSLVSSLAHSAIVHETSGITRCLLNDTANKQGDKELILNTEGINLYELFKYSNILELRRLYSNDIHTMAKTFGIEAALRVVEREIKDVFAVYGIEVDPRHLSLVADYMCFEGTYKPLNRIGMQSSSSPFQQMTFETSYKFLKDATMNGARDDLQSPSACLVVGKVV 1628 HSP 3 Score: 120.553 bits (301), Expect = 1.389e-26 Identity = 76/198 (38.38%), Postives = 110/198 (55.56%), Query Frame = 3
Query: 3141 LREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDII-----QLPFKITVG----DYYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKK------TFKQTLINITDNKDEDNQAVQTDLVNE 3689
L+ ++ +K + ++C G+AVGLLAAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPRLREILM+ S IKTP M VP+L A+ + KK T++ L ++I + F+I Y I F F Y+ + L+ + ++ E F + +K+ +F++ KD + Q + V E
Sbjct: 1132 LKTLLHLKWQRALCDPGDAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKAVKKVKQLKKKLTRVCLAEVIHKLDVEETFRIEEKSQKYQVYKIRFHFLPHEYYRQEKLLRPEGVLRFMETRFFKIMFDAIKRKSSKLASFREVGTRRATQKDHGDGEDQGNQVKE 1329
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Xenopus
Match: POLR1A (polymerase (RNA) I polypeptide A [Source:NCBI gene;Acc:100158635]) HSP 1 Score: 544.273 bits (1401), Expect = 7.907e-166 Identity = 380/1110 (34.23%), Postives = 571/1110 (51.44%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKD-SLIKRFNDYENMKSK---------------TLSDKQKNALKIMSSQ----TRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKR----MKIDENPKIRDFKSSYSQEELYGKFTNTDTSIFPEILREHLRLLWSNESENLQLILPMLKYNN-FKYPTDVFFMENMLINP----PMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREII--------KRKDSTAAEQYYQNIREGIL-----------------ELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPMVIGHNPLEVVENLKFQL----QNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW-----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYL-VYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKN-NLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNTKPVLSKV---LAKKIDELVVKLKRDFVEISPKDENLL----REIIAVKSEYSICPN 3188
L + F Y+ EE+R LS K +T+ ++ LG +NGLYD +LG + KE C C F +C GH G IE VYNPL FD L ++ CF+CH L + ++ + + ++ D + ++ EN+ + L D + LK Q + KN C ++ + K ++ +SAK P+CK ++ + N K+ + ++L + P +E ++ LW NE LQ + + N + D FF++ +++ P P+NRL GDQ+ + T N+ V++ + +++++ + + T + +NI L LQ VN+ +DS + +T PG+RQ ++KK+GLFR ++MGKRV++A RSVI PD I +EIG+P+VF +KLTYP+P+ P N L Q V+NGP+V+PGA V + G+R ++ + + E + QL QT+ + RH +GD L++NRQPTLHRPS+QAH + + P K LR+HY+NCKAYNADFDGDEMN H+PQS L +AEA L Q+ PKDG+PLAGLIQDH+++G M +R FTR + ++LVY GL + R LP PAI+ P LWTGKQ++S +L N IP L N++ W R + + ++II DG + G+ DK H G S GL ELYG E + K+LT L+RL T YL +Y GF+ ++D + + R NI+ + G ++ +FN+ E+A + A K + N +D FK + + +++ + C L FP+NNL LM Q+GAKG N G PL G+ L DL +R+ + QE FF MAGR+GLVDTAVKTS SGYLQRC+IKHLE + V +D TVRD+ ++IQF YG DGLDV + L +P IA+N ++ +K+ ++ VLS+V A++ + + + K +S + L +++ A K+ CPN
Sbjct: 12 LQGVSFGCYSAEEIRKLSVKALTNPHYMDN-LGNPMMNGLYDLALGPADGKEVCSTCAQDFNNCPGHLGHIELPLTVYNPLFFDKLYLLVRGSCFHCHLLTCHRSVIHLLLNQLKVLDRGAVHEVHELENVLHRLLEANAAATGNEIREELGDHTRKVLKKYKKQPGGISVKNT--CVTRNQLIGNFWKTQM---VSAKC------PNCKAPRSVVRKEHNSKLTIIITENRFKDLI--MGPKRGYLTPNSTKELIKALWENEGFFLQHLFSGITGNGESAFTPDTFFLDIIVVPPSRYRPVNRL-----GDQVFTNGHTVNLQAVLKDVSMIQKLLAVMAQDQEQMPEVTESGMITENIDRSFLATLMGQSLTDKLYNSWVRLQSHVNIVFDSDMDKLMTEKYPGIRQVLEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVMNGPNVHPGASMVISEDGSR--TILNSSNVSQREAVAKQLLTPSTGAQTLAVCRHVKNGDVLLLNRQPTLHRPSIQAHKARIL--PGEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYSLACTDEQYLVPKDGTPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYQGLTDKRGRVKVLP-PAILKPYPLWTGKQVVSTLLINVIPENLIPLNLTGKAKISGKAWVKEQPRPIPGFSPDSMCESQVIIRDGELLCGVLDKAHYGSSSFGLVHCCYELYGGETSGKLLTCLARLFTAYLQLYRGFTLGVEDILVLPYADAQRLNIVQQSLHCGGKAIRAAFNLSEAA-SDEEAHGKWQDAHLSKDQRDFNM---VDLKFKEEVNQFNNEINKVCMPFGLHRPFPENNLQLMVQSGAKGSTVNTI-------FTGGGGYSSPLQSTGKCLRYCGFSDL--------TNRYQFVISLQEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVIQFLYGEDGLDVPKTQFL-QPKQFPFIAENYEV-----IKQSKHLDEVLSRVNTEQAEQHNHAIYRWKARHPNLSQRISPFLLYSQKQMAAAKAS---CPN 1069 HSP 2 Score: 121.709 bits (304), Expect = 6.651e-27 Identity = 62/180 (34.44%), Postives = 106/180 (58.89%), Query Frame = 3
Query: 3972 IKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNS-----NKSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
I + L L FD+ S++ ++ + ++ +G+ + L+ ++ +K TEG N ELFKY +I +L + YSN+I M++ +GIEAA+ +E EIK VF Y I+V+ RHL L+AD++ G NR M + SSP Q+ +FET+ + + G D ++SP++ ++ G++V
Sbjct: 1448 ITIKLQLMKIHFDICSLVSSLAHSAIVHETSGITRCLLNDTANKQGDKELILNTEGINLYELFKYSNILELRRLYSNDIHTMAKTFGIEAALRVVEREIKDVFAVYGIEVDPRHLSLVADYMCFEGTYKPLNRIGMQSSSSPFQQMTFETSYKFLKDATMNGARDDLQSPSACLVVGKVV 1627 HSP 3 Score: 121.709 bits (304), Expect = 7.117e-27 Identity = 76/202 (37.62%), Postives = 113/202 (55.94%), Query Frame = 3
Query: 3141 LREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDII-----QLPFKITVG----DYYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKK------TFKQTLINITDNKDEDNQAVQTDLVNEDELN 3701
L+ ++ +K + ++C G+AVGLLAAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPRLREILM+ S IKTP M VP+L A+ + KK T++ L ++I + F+I Y I F F Y+ + L+ + ++ E F + +K+ +F++ KD + Q + E+++N
Sbjct: 1155 LKTLLHLKWQRALCDPGDAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKAVKKVKQLKKKLTRVCLAEVIHKLDVEETFRIEEKSQKYQVYKIRFHFLPHEYYRQEKLLRPEGVLRFMETRFFKIMFDAIKRKSSKLASFREVGTRRATQKDHGDGEDQGNQNEEEQVN 1356
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Xenopus
Match: POLR1A (polymerase (RNA) I polypeptide A [Source:NCBI gene;Acc:100158635]) HSP 1 Score: 542.732 bits (1397), Expect = 4.930e-165 Identity = 382/1114 (34.29%), Postives = 570/1114 (51.17%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKD-SLIKRFNDYENMKSK---------------TLSDKQKNALKIMSSQ----TRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKR----MKIDENPKIRDFKSSYSQEELYGKFTNTDTS-IFPEILREHLRLLWSNESENLQLILPMLKYNN-FKYPTDVFFMENMLINP----PMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREII--------KRKDSTAAEQYYQNIREGIL-----------------ELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPMVIGHNPLEVVENLKFQL-------QNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW-----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYL-VYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKN-NLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNTKPVLSKV---LAKKIDELVVKLKRDFVEISPKDENLL----REIIAVKSEYSICPN 3188
L + F Y+ EE+R LS K +T+ ++ LG +NGLYD +LG + KE C C F +C GH G IE VYNPL FD L ++ CF+CH L + ++ + + ++ D + ++ EN+ + L D + LK Q + KN C ++ + K ++ +SAK P+CK ++ + N K+ SS S + + P +E ++ LW NE LQ + + N + D FF++ +++ P P+NRL GDQ+ + T N+ V++ + +++++ + + T + +NI L LQ VN+ +DS + +T PG+RQ ++KK+GLFR ++MGKRV++A RSVI PD I +EIG+P+VF +KLTYP+P+ P N L Q V+NGP+V+PGA V + G+R ++ + + E + QL Q I+ RH +GD L++NRQPTLHRPS+QAH + + P K LR+HY+NCKAYNADFDGDEMN H+PQS L +AEA L Q+ PKDG+PLAGLIQDH+++G M +R FTR + ++LVY GL + R LP PAI+ P LWTGKQ++S +L N IP L N++ W R + + ++II DG + G+ DK H G S GL ELYG E + K+LT L+RL T YL +Y GF+ ++D + + R NI+ + G ++ +FN+ E+A + A K + N +D FK + + +++ + C L FP+NNL LM Q+GAKG N G PL G+ L DL +R+ + QE FF MAGR+GLVDTAVKTS SGYLQRC+IKHLE + V +D TVRD+ ++IQF YG DGLDV + L +P IA+N ++ +K+ ++ VLS+V A++ + + + K +S + L +++ A K+ CPN
Sbjct: 12 LQGVSFGCYSAEEIRKLSVKALTNPHYMDN-LGNPMMNGLYDLALGPADGKEVCSTCAQDFNNCPGHLGHIELPLTVYNPLFFDKLYLLVRGSCFHCHLLTCHRSVIHLLLNQLKVLDRGAVHEVHELENVLHRLLEANAAATGNEIREELGDHTRKVLKKYKKQPGGISVKNT--CVTRNQLIGNFWKTQM---VSAKC------PNCKAPRSVVRKEHNSKLTIIMSSKSMPLPKSEAPLAKRGYLTPNSTKELIKALWENEGFFLQHLFSGITGNGESAFTPDTFFLDIIVVPPSRYRPVNRL-----GDQVFTNGHTVNLQAVLKDVSMIQKLLAVMAQDQEQMPEVTESGMITENIDRSFLATLMGQSLTDKLYNSWVRLQSHVNIVFDSDMDKLMTEKYPGIRQVLEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVMNGPNVHPGASMVISEDGSR--TILNSSNVSQREAVAKQLLTPSTGAQTLAAKIVCRHVKNGDVLLLNRQPTLHRPSIQAHKARIL--PGEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYSLACTDEQYLVPKDGTPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYQGLTDKRGRVKVLP-PAILKPYPLWTGKQVVSTLLINVIPENLIPLNLTGKAKISGKAWVKEQPRPIPGFSPDSMCESQVIIRDGELLCGVLDKAHYGSSSFGLVHCCYELYGGETSGKLLTCLARLFTAYLQLYRGFTLGVEDILVLPYADAQRLNIVQQSLHCGGKAIRAAFNLSEAA-SDEEAHGKWQDAHLSKDQRDFNM---VDLKFKEEVNQFNNEINKVCMPFGLHRPFPENNLQLMVQSGAKGSTVNTI-------FTGGGGYSSPLQSTGKCLRYCGFSDL--------TNRYQFVISLQEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVIQFLYGEDGLDVPKTQFL-QPKQFPFIAENYEV-----IKQSKHLDEVLSRVNTEQAEQHNHAIYRWKARHPNLSQRISPFLLYSQKQMAAAKAS---CPN 1075 HSP 2 Score: 122.094 bits (305), Expect = 5.313e-27 Identity = 62/180 (34.44%), Postives = 106/180 (58.89%), Query Frame = 3
Query: 3972 IKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNS-----NKSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
I + L L FD+ S++ ++ + ++ +G+ + L+ ++ +K TEG N ELFKY +I +L + YSN+I M++ +GIEAA+ +E EIK VF Y I+V+ RHL L+AD++ G NR M + SSP Q+ +FET+ + + G D ++SP++ ++ G++V
Sbjct: 1490 ITIKLQLMKIHFDICSLVSSLAHSAIVHETSGITRCLLNDTANKQGDKELILNTEGINLYELFKYSNILELRRLYSNDIHTMAKTFGIEAALRVVEREIKDVFAVYGIEVDPRHLSLVADYMCFEGTYKPLNRIGMQSSSSPFQQMTFETSYKFLKDATMNGARDDLQSPSACLVVGKVV 1669 HSP 3 Score: 121.324 bits (303), Expect = 7.642e-27 Identity = 76/202 (37.62%), Postives = 113/202 (55.94%), Query Frame = 3
Query: 3141 LREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDII-----QLPFKITVG----DYYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKK------TFKQTLINITDNKDEDNQAVQTDLVNEDELN 3701
L+ ++ +K + ++C G+AVGLLAAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPRLREILM+ S IKTP M VP+L A+ + KK T++ L ++I + F+I Y I F F Y+ + L+ + ++ E F + +K+ +F++ KD + Q + E+++N
Sbjct: 1161 LKTLLHLKWQRALCDPGDAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKAVKKVKQLKKKLTRVCLAEVIHKLDVEETFRIEEKSQKYQVYKIRFHFLPHEYYRQEKLLRPEGVLRFMETRFFKIMFDAIKRKSSKLASFREVGTRRATQKDHGDGEDQGNQNEEEQVN 1362
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Xenopus
Match: POLR1A (polymerase (RNA) I polypeptide A [Source:NCBI gene;Acc:100158635]) HSP 1 Score: 538.11 bits (1385), Expect = 1.766e-163 Identity = 389/1135 (34.27%), Postives = 573/1135 (50.48%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKD-SLIKRFNDYENMKSK---------------TLSDKQKNALKIMSSQ----TRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKR----MKIDENPKIRDFKSSYSQEELYG--KFTNTDTSIF------------PEILREHLRLLWSNESENLQLILPMLKYNN-FKYPTDVFFMENMLINP----PMNRLPAVMNGDQMTESPETTNMCNVVRAEAALR--------------EIIKRKDSTAAEQYYQNIREGIL-----------------ELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPMVIGHNPLEVVENLKFQL-------QNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW-----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYL-VYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKN-NLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFC--VSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNTKPVLSKV---LAKKIDELVVKLKRDFVEISPKDENLL----REIIAVKSEYSICPN 3188
L + F Y+ EE+R LS K +T+ ++ LG +NGLYD +LG + KE C C F +C GH G IE VYNPL FD L ++ CF+CH L + ++ + + ++ D + ++ EN+ + L D + LK Q + KN C ++ + K ++ +SAK P+CK ++ + N K+ SS S G K DT + P +E ++ LW NE LQ + + N + D FF++ +++ P P+NRL GDQ+ + T N+ V++ + ++ E+ + S + +NI L LQ VN+ +DS + +T PG+RQ ++KK+GLFR ++MGKRV++A RSVI PD I +EIG+P+VF +KLTYP+P+ P N L Q V+NGP+V+PGA V + G+R ++ + + E + QL Q I+ RH +GD L++NRQPTLHRPS+QAH + + P K LR+HY+NCKAYNADFDGDEMN H+PQS L +AEA L Q+ PKDG+PLAGLIQDH+++G M +R FTR + ++LVY GL + R LP PAI+ P LWTGKQ++S +L N IP L N++ W R + + ++II DG + G+ DK H G S GL ELYG E + K+LT L+RL T YL +Y GF+ ++D + + R NI+ + G ++ +FN+ E+A + A K + N +D FK + + +++ + C L FP+NNL LM Q+GAKG N T+ GR LP L P GF +R+ + QE FF MAGR+GLVDTAVKTS SGYLQRC+IKHLE + V +D TVRD+ ++IQF YG DGLDV + L +P IA+N ++ +K+ ++ VLS+V A++ + + + K +S + L +++ A K+ CPN
Sbjct: 12 LQGVSFGCYSAEEIRKLSVKALTNPHYMDN-LGNPMMNGLYDLALGPADGKEVCSTCAQDFNNCPGHLGHIELPLTVYNPLFFDKLYLLVRGSCFHCHLLTCHRSVIHLLLNQLKVLDRGAVHEVHELENVLHRLLEANAAATGNEIREELGDHTRKVLKKYKKQPGGISVKNT--CVTRNQLIGNFWKTQM---VSAKC------PNCKAPRSVVRKEHNSKLTIIMSSKSSASSKGGNKPAEGDTEVADSSQLAKRGYLTPNSTKELIKALWENEGFFLQHLFSGITGNGESAFTPDTFFLDIIVVPPSRYRPVNRL-----GDQVFTNGHTVNLQAVLKDVSMIQKLLAVMAQDQEQMPEVTESGMSLKEGEVTENIDRSFLATLMGQSLTDKLYNSWVRLQSHVNIVFDSDMDKLMTEKYPGIRQVLEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVMNGPNVHPGASMVISEDGSR--TILNSSNVSQREAVAKQLLTPSTGAQTLAAKIVCRHVKNGDVLLLNRQPTLHRPSIQAHKARIL--PGEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYSLACTDEQYLVPKDGTPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYQGLTDKRGRVKVLP-PAILKPYPLWTGKQVVSTLLINVIPENLIPLNLTGKAKISGKAWVKEQPRPIPGFSPDSMCESQVIIRDGELLCGVLDKAHYGSSSFGLVHCCYELYGGETSGKLLTCLARLFTAYLQLYRGFTLGVEDILVLPYADAQRLNIVQQSLHCGGKAIRAAFNLSEAA-SDEEAHGKWQDAHLSKDQRDFNM---VDLKFKEEVNQFNNEINKVCMPFGLHRPFPENNLQLMVQSGAKGSTVN--------------------TMQGRYLPW--NSALFPYC-GFSDLTNRYQFVISLQEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVIQFLYGEDGLDVPKTQFL-QPKQFPFIAENYEV-----IKQSKHLDEVLSRVNTEQAEQHNHAIYRWKARHPNLSQRISPFLLYSQKQMAAAKAS---CPN 1088 HSP 2 Score: 122.094 bits (305), Expect = 4.851e-27 Identity = 62/180 (34.44%), Postives = 106/180 (58.89%), Query Frame = 3
Query: 3972 IKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNS-----NKSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
I + L L FD+ S++ ++ + ++ +G+ + L+ ++ +K TEG N ELFKY +I +L + YSN+I M++ +GIEAA+ +E EIK VF Y I+V+ RHL L+AD++ G NR M + SSP Q+ +FET+ + + G D ++SP++ ++ G++V
Sbjct: 1468 ITIKLQLMKIHFDICSLVSSLAHSAIVHETSGITRCLLNDTANKQGDKELILNTEGINLYELFKYSNILELRRLYSNDIHTMAKTFGIEAALRVVEREIKDVFAVYGIEVDPRHLSLVADYMCFEGTYKPLNRIGMQSSSSPFQQMTFETSYKFLKDATMNGARDDLQSPSACLVVGKVV 1647 HSP 3 Score: 87.8113 bits (216), Expect = 1.226e-16 Identity = 60/186 (32.26%), Postives = 90/186 (48.39%), Query Frame = 3
Query: 3141 LREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQLPFKITVGDYYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKK------------TFKQTLINITDNKDEDNQ 3662
L+ ++ +K + ++C G+AVGLLAAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPR P + L C L+ KL +++ ++ K Y I F F Y+ + L+ + ++ E F + +K+ T + T + D +D+ NQ
Sbjct: 1174 LKTLLHLKWQRALCDPGDAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRY------------VPKVIAESLLC----------LQVIHKLDVEETFRIEEKSQKYQVYKIRFHFLPHEYYRQEKLLRPEGVLRFMETRFFKIMFDAIKRKSSKLASFREVGTRRATQKDHGDGEDQGNQ 1337
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Mouse
Match: Polr1a (polymerase (RNA) I polypeptide A [Source:MGI Symbol;Acc:MGI:1096397]) HSP 1 Score: 575.474 bits (1482), Expect = 7.635e-177 Identity = 385/1102 (34.94%), Postives = 573/1102 (52.00%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQ---------------GLLESICDLGQIKDSLIKRFND------YENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMK-----------IDENPKIRDFKSSYSQEEL---YGKFTNTDTS-------IFPEILREHLRLLWSNES-------ENLQLILPMLKYNNFKYPTDVFFMENMLINP----PMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREII-------------------KRKDSTAA-EQYYQNIREG----------ILELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPM--VIGHNPLEVVENL----KFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW-----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYL-VYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLM----EFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKN-NLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQ-LMEKRYLKEPRNTKPVLSKVLAKKI 3077
L I F Y+ EE++ LS K IT+ ++ LG + NGLYD +LG + KE C C F +C+GH G I+ VYNP LFD L ++ C CH L + G L+++ +L +I ++ D E ++ T Q N L + + C F K+ H++AK+ P CK + I P KS EL + D + + P +EHL +W NE L I P +N +FF++ +++ P P+NRL GDQM + +T N+ V++ +R+++ K DS+ A ++ + + G + LQ VN+ +DS + + PG+RQ ++KK+GLFR ++MGKRV++A RSVI PD I +EIG+P+VF +KLTYP+P+ P N L Q V+NGP+V+PGA V + G+R + V V + L + T ++ RH +GD L++NRQPTLHRPS+QAH + + P+ K LR+HY+NCKAYNADFDGDEMN H+PQS L +AEA +L Q+ PKDG PLAGLIQDH+++G M +R FTR + ++LVY GL + R KL PAI+ P LWTGKQ++S +L N IP N+S + W R D + + ++II +G + G+ DK H G S GL E+YG E + ++LT L+RL T YL +Y GF+ ++D + + + R+ I+ + G +V + ++P E +G+ + + Q D ID FK ++Y +++ + C L +FP+NNL +M Q+GAKG N +I+ LG IE+EG+R PL G++LP F+ Y+ P + GF RF++G++P E FF MAGR+GLVDTAVKTS SGYLQRC+IKHLE + + +D TVRD+ +++QF YG DGLD+ + L +P +A N + +M+ ++L E VLS+ +K+
Sbjct: 12 LQGISFGMYSAEELKKLSVKSITNPRYVD-YLGNPSANGLYDLALGPADSKEVCATCVQDFNNCSGHLGHIDLPLTVYNPFLFDKLYLLLRGSCLSCHMLTCPRAAIYLLISQLRVLEVGALQAVYELERILSRFLEETGDPSAFEIQEELEEYTSKILQNNLLGSQGTHVKNVCESRSKLVAQFWKT-------HMAAKQC-----PHCKTGRSVVRKEHNSKLIITYPATVHKKSDQEGTELPEGVPEAPGIDKAQMGKRGYLTPSSAQEHLFAIWKNEGFFLNYLFSGLDDIGPESSFN-----PSMFFLDFIVVPPSRYRPVNRL-----GDQMFTNGQTVNLQAVMKDAVLIRKLLALMAQEQKLPCEMTELTIDKENDSSVAIDRSFLGLLPGPSLTDKLYNIWIRLQSHVNIVFDSEMDKLMLEKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSRTALSSVDAAQREAVAKQLLTPATGAPKPQGTKVVCRHVKNGDILLLNRQPTLHRPSIQAHRARIL--PEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYRGLTDKVGR-VKLFPPAILKPFPLWTGKQVVSTLLINIIPEDYAPLNLSGKAKIGSKAWVKEKPRPIPDFDPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGRVLTCLARLFTAYLQLYRGFTLGVEDILVKPNADVVRQRIIEESTQCGPQAVKAALSLPETASCDEIQGKWQDAHLSKDQRD--------FNMIDMKFKEEVNHYSNEINKACMPLGLHRQFPENNLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIRPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVIQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFL-QPKQFPFLAGNYEVIMKSKHLHE------VLSRADPQKV 1072 HSP 2 Score: 135.576 bits (340), Expect = 2.822e-31 Identity = 79/199 (39.70%), Postives = 117/199 (58.79%), Query Frame = 3
Query: 3051 LSKVLAKKID----ELVVKLKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQ---LPFKITVGD------YYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKK 3608
+S+ KKID E + +R + + S + LR ++ +K + S+C GEAVGLLAAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPRLREILM+ S IKTP M VP+ A+ S+ K+ T++ L +++Q + +G+ Y + F F Y+ + L+ ++++ E F + + +KK
Sbjct: 1166 VSETFEKKIDDFSQEWAAQAERSYKK-SELSLDRLRTLLQLKWQRSLCDPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVFDTKKALKKVKSLKKRLTRVCLGEVLQKVDIQESFCMGEKRNKFQVYELRFQFLPHAYYQQEKCLRPEDILHFMETRFFKLLMEAIKK 1363 HSP 3 Score: 127.872 bits (320), Expect = 6.780e-29 Identity = 66/180 (36.67%), Postives = 104/180 (57.78%), Query Frame = 3
Query: 3972 IKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNSNKSKR-----FATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
+ + L L FD+ S+++++ ++ + G+ + L+ + SK TEG N ELFKY I DL + YSN+I M+ YGIEAA+ IE EIK VF Y I V+ RHL L+AD++ G NR + + SSPLQ+ +FET+ + + G+ D +KSP++ ++ G++V
Sbjct: 1524 VTVKLPLMKINFDMSSLVVSLAHKAIVYTTKGITRCLLNETTNSKNEKELVLNTEGINLPELFKYSEILDLRRLYSNDIHAMANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIQSSSSPLQQMTFETSFQFLKQATMMGSHDELKSPSACLVVGKVV 1703
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Mouse
Match: Polr3a (polymerase (RNA) III (DNA directed) polypeptide A [Source:MGI Symbol;Acc:MGI:2681836]) HSP 1 Score: 388.652 bits (997), Expect = 4.132e-112 Identity = 353/1210 (29.17%), Postives = 541/1210 (44.71%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITS--LISIE----PVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKI-MSSQTRK--NCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQ-EELYGKFTNTDTSIFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAVMNG--DQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDSGRHRELTG---------SQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQ-----------IGNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQR-EFKLPKPAIIFPNQLWTGKQILSIVLK----NSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQK-GLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKD-----GFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNTKPVLSKVLAKKIDELVVKLKRDFV--------EISPKDENLLREIIAVKSEYSICPN-------------------------------------GEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDI 3446
+ ICF +PEE+R + ++ S L S + P+L G+ D +G+ PC C + DC GH+G I+ ++ F + ++ +C C + +Q + D +K L+ QK LK +S + RK CH C + KK +I +H K ++ +P ++ F+++ +E+ ++ P ++ L L +E++ L+L + P+D+ L+ PP+ P+V++ E T + ++ L ++IK+ + A+ Q I E LQ + + +S EL+G G Q++ K G FR N+ GKRV+F+ R+VISPDP + +DE+ VP+ LT+PE +N N +FL + V NGPDV+PGA Q GNR M + LKF I+ RH + GD ++ NRQP+LH+ S+ AH+ + +T R + C YNADFDGDEMN H PQ+ A+AEA +L+ TP++G PL IQD + + L+D+ F R + ++ L +++ + +LP P I+ P LWTGKQI S++L+ N + A L + + G + L ++D + I + + G DK LG K + L +G A ++ L+RL YL GFS I D G L E I + + GK L + G A +E + R+ C LRE N+ L M G+KG N++++ +G I G R P R+LP F+++ P ++GF + F SGL P E FF +MAGR+GLVDTAVKT+ +GY+QR L+K LED+ +D TVR + +IIQF YG DGLD +P + + NI+ + P T+P LSK E ++K K +F+ EI + + +I + +Y I N G AVG L AQS+GEP TQMTL TFH AG + MN+TLG+PR++EI+ S I TP + + DDA + A V + K L +I
Sbjct: 15 ISHICFGMKSPEEMRQQAHIQVVSKNLYSQDNNHAPLL-----YGVLDHRMGTSEKDRPCETCGKNLADCLGHYGYIDLELPCFHVGYFRAVIGILQMICKTCCHIMLSQEEKQQFLDF----------------LKRPGLTYLQKRGLKKKISDKCRKKSTCHYCGAFNGTVKKCGLLKI-IHEKYKTNKKVVDPIVSNF-------LQSFETAIEHNKEVEPLLGRAQENLNPLVV---LNLFKRIPAEDVPLLLMNPESGK---PSDLILTR--LLVPPLCIRPSVVSDLKSGTNEDDLTMKLTEII----FLNDVIKKHRISGAKT--QMIMEDWDFLQLQCALYINS----ELSGIPLNMAPKKWTRGFVQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLRIDEVAVPVHVAKILTFPEKVNKANINFLRKLVRNGPDVHPGANFIQQRHMQMKRFLKYGNREKM---------AQELKF------GDIVERHLIDGDVVLFNRQPSLHKLSIMAHLARV---KPHRTFRFNECVCTPYNADFDGDEMNLHLPQTEEAKAEALVLMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTFFDRAKACQIIASILVGKDEKIKVRLPPPTILKPVTLWTGKQIFSVILRPSDDNPVRANL-----------RTKGKQYCGKGEDLCVNDSYVTIQNSELMCGSMDKGTLGSGSKNNIFYILLRDWGQNEAADAMSRLARLAPVYLSNRGFSIGIGDVTPGQGLLKAKYELLNAGYKKCDEYIEALNTGK-----LQQQPGCTAEETLEALILKELSVIRDHAG------------------SAC---LRELDKSNSPLTMALCGSKGSFINISQMIACVGQQAISGSRVPDGFENRSLPHFEKHSKLPAAKGFVANSFYSGLTPTEFFFHTMAGREGLVDTAVKTAETGYMQRRLVKSLEDLCSQYDLTVRSSTGDIIQFIYGGDGLDPAAMEGKDEPLEFKRVLDNIKAV------YPCQTEPALSKNDLTLTAEAIMK-KNEFLCCQDSFLQEIKTFIKGVSEKIKKTRDKYGINDNGTTEPRVLYQLDRITPTQIEKFLETCRDKYMRAQMEPGSAVGALCAQSIGEPGTQMTLKTFHFAGVASMNITLGVPRIKEIIN-ASKAISTPIITAQLDKDDDA-DYARLVKGRIEKTLLGEI 1113 HSP 2 Score: 57.3806 bits (137), Expect = 1.630e-7 Identity = 44/161 (27.33%), Postives = 76/161 (47.20%), Query Frame = 3
Query: 4047 VISSVAGVEKALIF---NSNKSK-RFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTN-HSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTN 4514
V+ + V +A+I S K K + EG N + + + SNN ++ + GIEAA + I NEI+ ++ + ++ RH+ L++D +T+ G+V R + S L ASFE + + +G D + + I+ G + NIGT
Sbjct: 1200 VVQGIPEVSRAVIHIDEQSGKEKFKLLVEGDNLRAVMATHGVKG-TRTTSNNTYEVEKTLGIEAARTTIINEIQYTMVNHGMSIDRRHVMLLSDLMTYKGEVLGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMG-IPMNIGTG 1358
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Mouse
Match: Polr2a (polymerase (RNA) II (DNA directed) polypeptide A [Source:MGI Symbol;Acc:MGI:98086]) HSP 1 Score: 352.829 bits (904), Expect = 1.199e-98 Identity = 292/972 (30.04%), Postives = 454/972 (46.71%), Query Frame = 3
Query: 90 ICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNC----HQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYG--KFTNTDTS-----IFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAV-MNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDS---GRHRELTGS-QP--GLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQI---GNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEI--DTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGL 2936
+ F +P+E++ +S E G+ + GL D G + C+ C + +C GHFG IE +K V++ K ++ VCF+C +L L++S + +IKD L +K+ +K + KN + NK V + ++T ++KG C R + P+IR S ELY K N D+ + PE + E + + E +L M +Y + + +L PP++ PAV M G + T + ++V+ LR R + A + I E + LQ V D+ G R + S +P L+Q++ K+G R N+MGKRV+F+ R+VI+PDP + +D++GVP + +T+ E + P N D L + V G YPGA Y I G+R + P ++ LQ V RH GD +I NRQPTLH+ SM H ++ + T R++ S YNADFDGDEMN H PQSL +AE + L V TP+ P+ G++QD + A K RD R E ++L L + + K+P+PAI+ P LWTGKQI S++ IP + + P SG + + D ++++ +G + GI K LG S L G ++ +N+ ++ +L+ G + I D + +N + K + + K+ N E +L + F N++ I D KT SS + +L E NN M +GAKG + N++++ +G +EGKR P RTLP F + D P SRGF + +++GL P E FF +M GR+GL+DTAVKT+ +GY+QR LIK +E + V +D TVR++++ ++Q RYG DGL
Sbjct: 21 VQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKL-----LVDS--NNPKIKDIL-----------AKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLT--------KEKGHGGCGRYQ----PRIR-----RSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEEC----FVLGM----EPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLR----RNEQNGAAAHV--IAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGA-KYIIRDNGDRIDLRFHPKPSDL------HLQTGYKV--ERHMCDGDIVIFNRQPTLHKMSMMGHRVRIL---PWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNL----LMFLSTWDGKVPQPAILKPRPLWTGKQIFSLI----IPGHINCIRTHSTHPDDED----SGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHN---NELEPTPGNTLRQ--------TFENQVNRILNDARDKTGSS---------AQKSLSEY---NNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGL 896 HSP 2 Score: 81.2629 bits (199), Expect = 9.497e-15 Identity = 47/80 (58.75%), Postives = 58/80 (72.50%), Query Frame = 3
Query: 3138 LLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILA 3377
LL EI + K +I GE VG LAAQS+GEP+TQMTLNTFH AG S NVTLG+PRL+E++ + S K KTPS+ V +L
Sbjct: 1068 LLGEIES-KFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINI-SKKPKTPSLTVFLLG 1145 HSP 3 Score: 68.9366 bits (167), Expect = 5.509e-11 Identity = 41/128 (32.03%), Postives = 66/128 (51.56%), Query Frame = 3
Query: 4116 TEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNH-SSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
T+G + + + + D + SN+I+++ + GIEA +E E+ V VN RHL L+ D +T G + A RH + + PL + SFE + V++E +G D +K + NIM GQL
Sbjct: 1338 TDGVSLMRVLSEKDV-DPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLA 1464
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Mouse
Match: Polr2a (polymerase (RNA) II (DNA directed) polypeptide A [Source:MGI Symbol;Acc:MGI:98086]) HSP 1 Score: 351.673 bits (901), Expect = 2.683e-98 Identity = 292/972 (30.04%), Postives = 454/972 (46.71%), Query Frame = 3
Query: 90 ICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNC----HQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYG--KFTNTDTS-----IFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAV-MNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDS---GRHRELTGS-QP--GLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQI---GNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEI--DTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGL 2936
+ F +P+E++ +S E G+ + GL D G + C+ C + +C GHFG IE +K V++ K ++ VCF+C +L L++S + +IKD L +K+ +K + KN + NK V + ++T ++KG C R + P+IR S ELY K N D+ + PE + E + + E +L M +Y + + +L PP++ PAV M G + T + ++V+ LR R + A + I E + LQ V D+ G R + S +P L+Q++ K+G R N+MGKRV+F+ R+VI+PDP + +D++GVP + +T+ E + P N D L + V G YPGA Y I G+R + P ++ LQ V RH GD +I NRQPTLH+ SM H ++ + T R++ S YNADFDGDEMN H PQSL +AE + L V TP+ P+ G++QD + A K RD R E ++L L + + K+P+PAI+ P LWTGKQI S++ IP + + P SG + + D ++++ +G + GI K LG S L G ++ +N+ ++ +L+ G + I D + +N + K + + K+ N E +L + F N++ I D KT SS + +L E NN M +GAKG + N++++ +G +EGKR P RTLP F + D P SRGF + +++GL P E FF +M GR+GL+DTAVKT+ +GY+QR LIK +E + V +D TVR++++ ++Q RYG DGL
Sbjct: 21 VQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKL-----LVDS--NNPKIKDIL-----------AKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLT--------KEKGHGGCGRYQ----PRIR-----RSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEEC----FVLGM----EPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLR----RNEQNGAAAHV--IAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGA-KYIIRDNGDRIDLRFHPKPSDL------HLQTGYKV--ERHMCDGDIVIFNRQPTLHKMSMMGHRVRIL---PWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNL----LMFLSTWDGKVPQPAILKPRPLWTGKQIFSLI----IPGHINCIRTHSTHPDDED----SGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHN---NELEPTPGNTLRQ--------TFENQVNRILNDARDKTGSS---------AQKSLSEY---NNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGL 896 HSP 2 Score: 81.2629 bits (199), Expect = 1.029e-14 Identity = 47/80 (58.75%), Postives = 58/80 (72.50%), Query Frame = 3
Query: 3138 LLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILA 3377
LL EI + K +I GE VG LAAQS+GEP+TQMTLNTFH AG S NVTLG+PRL+E++ + S K KTPS+ V +L
Sbjct: 1068 LLGEIES-KFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINI-SKKPKTPSLTVFLLG 1145 HSP 3 Score: 68.9366 bits (167), Expect = 5.913e-11 Identity = 41/128 (32.03%), Postives = 66/128 (51.56%), Query Frame = 3
Query: 4116 TEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNH-SSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
T+G + + + + D + SN+I+++ + GIEA +E E+ V VN RHL L+ D +T G + A RH + + PL + SFE + V++E +G D +K + NIM GQL
Sbjct: 1338 TDGVSLMRVLSEKDV-DPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLA 1464
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Mouse
Match: Polr3a (polymerase (RNA) III (DNA directed) polypeptide A [Source:MGI Symbol;Acc:MGI:2681836]) HSP 1 Score: 228.024 bits (580), Expect = 1.555e-62 Identity = 189/659 (28.68%), Postives = 306/659 (46.43%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITS--LISIE----PVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKI-MSSQTRK--NCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQ-EELYGKFTNTDTSIFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAVMNG--DQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDSGRHRELTG---------SQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQ-----------IGNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQR-EFKLPKPAIIFPNQLWTGKQILSIVLKNS 1958
+ ICF +PEE+R + ++ S L S + P+L G+ D +G+ PC C + DC GH+G I+ ++ F + ++ +C C + +Q + D +K L+ QK LK +S + RK CH C + KK +I +H K ++ +P ++ F+++ +E+ ++ P ++ L L +E++ L+L + P+D+ L+ PP+ P+V++ E T + ++ L ++IK+ + A+ Q I E LQ + + +S EL+G G Q++ K G FR N+ GKRV+F+ R+VISPDP + +DE+ VP+ LT+PE +N N +FL + V NGPDV+PGA Q GNR M + LKF I+ RH + GD ++ NRQP+LH+ S+ AH+ + +T R + C YNADFDGDEMN H PQ+ A+AEA +L+ TP++G PL IQD + + L+D+ F R + ++ L +++ + +LP P I+ P LWTGKQI S++L+ S
Sbjct: 15 ISHICFGMKSPEEMRQQAHIQVVSKNLYSQDNNHAPLL-----YGVLDHRMGTSEKDRPCETCGKNLADCLGHYGYIDLELPCFHVGYFRAVIGILQMICKTCCHIMLSQEEKQQFLDF----------------LKRPGLTYLQKRGLKKKISDKCRKKSTCHYCGAFNGTVKKCGLLKI-IHEKYKTNKKVVDPIVSNF-------LQSFETAIEHNKEVEPLLGRAQENLNPLVV---LNLFKRIPAEDVPLLLMNPESGK---PSDLILTR--LLVPPLCIRPSVVSDLKSGTNEDDLTMKLTEII----FLNDVIKKHRISGAKT--QMIMEDWDFLQLQCALYINS----ELSGIPLNMAPKKWTRGFVQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLRIDEVAVPVHVAKILTFPEKVNKANINFLRKLVRNGPDVHPGANFIQQRHMQMKRFLKYGNREKM---------AQELKF------GDIVERHLIDGDVVLFNRQPSLHKLSIMAHLARV---KPHRTFRFNECVCTPYNADFDGDEMNLHLPQTEEAKAEALVLMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTFFDRAKACQIIASILVGKDEKIKVRLPPPTILKPVTLWTGKQIFSVILRPS 608
BLAST of DNA-directed RNA polymerase subunit vs. UniProt/SwissProt
Match: sp|P91875|RPA1_DROME (DNA-directed RNA polymerase I subunit RPA1 OS=Drosophila melanogaster OX=7227 GN=RpI1 PE=1 SV=2) HSP 1 Score: 603.594 bits (1555), Expect = 0.000e+0 Identity = 434/1322 (32.83%), Postives = 655/1322 (49.55%), Query Frame = 3
Query: 96 FSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLND-KEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESI-CDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCH---------------NKSLVFKKSRKCEITVHISAKESRDKGEPSC-KRMKIDE--NPKIRDFKSSYSQEELYGKFTNT---DTSIFPEILREHLRLLWSNESENLQLILPMLKYNNF------KYPTDVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREIIK------RKDSTAAEQYYQNIR---------EGILELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVVENL-KFQLQN--NQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKL--------KLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETC-KNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVI----------------NSVSLSKPDFMEVIAKNIQLME----KRYLKEPRNTKPVLSKVLAKKIDELV---VKLKRDFVEISPK------DENLLREIIAVKSE--------YSICPN-------------------------------------GEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTL---------------GIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSV-LKKFTKLYLKDIIQLPFKITVGD----YYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKKT 3611
F+ +T +E+R LS ++ + I+ + LG GLYD +GS +PC C L DC GH G IE VYNP + + + C +C++L E I L I I + E KS+ + +N + I KN H N S K S + SA + K C K M+ + ++ + + +E G T + IF + R +LR +++N E L+L++P+L +N + P D+FFM+ + + PP R P M GD + +P+T N++ L ++K K + A+ YQ ++ L LQ V+V D RE+ + GL+Q I+KK GL RS++MGKRVN+A R+VI+PDP I +DEIG+P +F KL+YP P+ N L + V+NGPDV+PGA Q N F I + E+L K L N + I++RH L+GD L++NRQP+LH+PS+ H + +H KT R+HYSNCKAYNADFDGDEMN HYPQS +A+AEA LVNV + PKDG+PL GLIQDH+I+G K+ +R F R + LV+ GL + +++ KL P I+ P LW+GKQILS ++ N IP G E N+ W +S + LS+ ++ I +G + G+ DK G + GL + ELYG +V+ +LT +++ T +L GF+ +KD + + + R+ I+ + R++G+++V + + E ++ ME A + K KFR +D +K+L Y + + TC L KFP NNL LM +GAKG N +I+ LG IE+EGKR PL + G++LPSF ++ P S GF RFM+G++PQ+ FF MAGR+GL+DTAVKTS SGYLQRCLIKHLE ++V +D TVRD+ ++++QF YG DGLD++ N+ ++ +P ++++ QL + +++++ KP + E + V++KR EI+ K DE LL+ +E Y+ CP+ + + + +S+ P + L + G+ +TL GIPRLREILML S IKTPSMD+PI + L T L + + + +T+ Y + F F + YK ++ K +I F + + + K
Sbjct: 18 FAVFTDQEIRKLSVVKVITGITFD-ALGHAIPGGLYDIRMGSYGRCMDPCGTC-LKLQDCPGHMGHIELGTPVYNPFFIKFVQRLLCIFCLHCYKLQMKDHECEIIMLQLRLIDAGYIIEAQELELFKSEIVCQNTENLVAI------KNGDMVHPHIAAMYKLLEKNEKNSSNSTKTSCSLRTAITHSALQRLGKKCRHCNKSMRFVRYMHRRLVFYVTLADIKERVGTGAETGGQNKVIFADECRRYLRQIYANYPELLKLLVPVLGLSNTDLTQGDRSPVDLFFMDTLPVTPPRAR-PLNMVGDMLKGNPQTDIYINIIENNHVLNVVLKYMKGGQEKLTEEAKAAYQTLKGETAHEKLYTAWLALQMSVDVLLDVNMSREMKSGE-GLKQIIEKKSGLIRSHMMGKRVNYAARTVITPDPNINVDEIGIPDIFAKKLSYPVPVTEWNVTELRKMVMNGPDVHPGANYIQDKNGFTTYIPADNASKRESLAKLLLSNPKDGIKIVHRHVLNGDVLLLNRQPSLHKPSIMGHKARILHG--EKTFRLHYSNCKAYNADFDGDEMNAHYPQSEVARAEAYNLVNVASNYLVPKDGTPLGGLIQDHVISGVKLSIRGRFFNREDYQQLVFQGL-SQLKKDIKLLPPTILKPAVLWSGKQILSTIIINIIPEGYERINLDSFAKIAGKNWNVSRPRPPICGTNPEGNDLSESQVQIRNGELLVGVLDKQQYGATTYGLIHCMYELYGGDVSTLLLTAFTKVFTFFLQLEGFTLGVKDILVTDVADRKRRKIIRECRNVGNSAVAAALELE-DEPPHDELVEKME-AAYVKDSKFR---VLLDRKYKSLLDGYTNDINSTCLPRGLITKFPSNNLQLMVLSGAKGSMVNTMQISCLLGQIELEGKRPPLMISGKSLPSFTSFETSPKSGGFIDGRFMTGIQPQDFFFHCMAGREGLIDTAVKTSRSGYLQRCLIKHLEGLSVHYDLTVRDSDNSVVQFLYGEDGLDILKSKFFNDKFCADFLTQNATAILRPAQLQLMKDEEQLAKVQRHEKHIRSWEKKKPAKLRAAFTHFSEELREEVEVKRPN-EINSKTGRRRFDEGLLKLWKKADAEDKALYRKKYARCPDPTVAVYKQDLYYGSVSERTRKLITDYAKRKPALKETIADIMRVKTIKSLAAPGEPVGLIAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMLASSNIKTPSMDIPIKPGQQHQAEKLRINLNSVTLANLLEYVHVSTGLTLDPERSYEYDMRFQFLPREVYKEDYGVRPKHIIKYMHQTFFKQLIRAILKV 1320 HSP 2 Score: 135.191 bits (339), Expect = 2.242e-30 Identity = 72/186 (38.71%), Postives = 111/186 (59.68%), Query Frame = 3
Query: 3969 W--IKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNSNKSKRF-ATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTNA 4517
W +KL L++ +++ D+ SI+ + V+ V +++A+I+ N + T+G N E+F+++ I DLN+ YSN+I ++R YGIEAA I E+ VFK Y I V+ RHL LIAD++T G +R M + SSPLQ+ SFE++L + +G D + SP+S +M G V N GT A
Sbjct: 1450 WCQVKLNLSVRYQKPDLTSIIRELAGKSVVHQVQHIKRAIIYKGNDDDQLLKTDGINIGEMFQHNKILDLNRLYSNDIHAIARTYGIEAASQVIVKEVSNVFKVYGITVDRRHLSLIADYMTFDGTFQPLSRKGMEHSSSPLQQMSFESSLQFLKSAAGFGRADELSSPSSRLMVGLPVRN-GTGA 1634
BLAST of DNA-directed RNA polymerase subunit vs. UniProt/SwissProt
Match: sp|O95602|RPA1_HUMAN (DNA-directed RNA polymerase I subunit RPA1 OS=Homo sapiens OX=9606 GN=POLR1A PE=1 SV=2) HSP 1 Score: 575.859 bits (1483), Expect = 4.736e-176 Identity = 388/1093 (35.50%), Postives = 566/1093 (51.78%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQ---------------GLLESICDLGQIKDSLIKRFND------YENMKSKTLSDKQKNALKIMSSQTR--------------------KNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYGKFTNTDTSIFPEILREHLRLLWSNESENLQLILPMLKYNNF--KYPTDVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREII-------------------KRKDS-TAAEQYYQNIREG----------ILELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPMVIGHNPLEVVENLKFQL--------QNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW-----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYL-VYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLM----EFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKN-NLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQ-LMEKRYLKEPRNTKPVLSKVLAKK 3074
L I F Y+ EE++ LS K IT+ ++ LG + NGLYD +LG + KE C C F +C+GH G IE VYNPLLFD L ++ C CH L + G L+++ +L +I + ++ D E ++ T Q N L + + K C C V +K ++T+ A R G+ + + I+E +Q G T T REHL LW NE L + + + ++ VFF+ + L+ PP P GDQM + +T N+ V++ +R+++ + KDS A ++ + + G + LQ VN+ +DS + + PG+RQ ++KK+GLFR ++MGKRV++A RSVI PD I +EIG+P+VF +KLTYP+P+ P N L Q V+NGP+V+PGA V + G+R + + E + QL + T I+ RH +GD L++NRQPTLHRPS+QAH + + P+ K LR+HY+NCKAYNADFDGDEMN H+PQS L +AEA +L Q+ PKDG PLAGLIQDH+++G M R FTR ++LVY GL + R KL P+I+ P LWTGKQ++S +L N IP N+S W R + + ++II +G + G+ DK H G S GL E+YG E + K+LT L+RL T YL +Y GF+ ++D + + R+ I+ + G +V + N+P E RG+ + + Q D ID FK ++Y +++ + C L +FP+N+L +M Q+GAKG N +I+ LG IE+EG+R PL G++LP F+ Y+ P + GF RF++G+KP E FF MAGR+GLVDTAVKTS SGYLQRC+IKHLE + V +D TVRD+ +++QF YG DGLD+ + L +P +A N + +M+ ++L E VLS+ KK
Sbjct: 12 LQGISFGMYSAEELKKLSVKSITNPRYLDS-LGNPSANGLYDLALGPADSKEVCSTCVQDFSNCSGHLGHIELPLTVYNPLLFDKLYLLLRGSCLNCHMLTCPRAVIHLLLCQLRVLEVGALQAVYELERILNRFLEENPDPSASEIREELEQYTTEIVQNNLLGSQGAHVKNVCESKSKLIALFWKAHMNAKRCPHCKTGRSVVRKEHNSKLTITFPAMVHRTAGQKDSEPLGIEE-----------AQIGKRGYLTPTSA-------REHLSALWKNEGFFLNYLFSGMDDDGMESRFNPSVFFL-DFLVVPPSRYRPVSRLGDQMFTNGQTVNLQAVMKDVVLIRKLLALMAQEQKLPEEVATPTTDEEKDSLIAIDRSFLSTLPGQSLIDKLYNIWIRLQSHVNIVFDSEMDKLMMDKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSR--TALSAVDMTQREAVAKQLLTPATGAPKPQGTKIVCRHVKNGDILLLNRQPTLHRPSIQAHRARIL--PEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGASMTTRGCFFTREHYMELVYRGLTDKVGR-VKLLSPSILKPFPLWTGKQVVSTLLINIIPEDHIPLNLSGKAKITGKAWVKETPRSVPGFNPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGKVLTCLARLFTAYLQLYRGFTLGVEDILVKPKADVKRQRIIEESTHCGPQAVRAALNLPEAASYDEVRGKWQDAHLGKDQRD--------FNMIDLKFKEEVNHYSNEINKACMPFGLHRQFPENSLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFL-QPKQFPFLASNYEVIMKSQHLHE------VLSRADPKK 1064 HSP 2 Score: 137.502 bits (345), Expect = 5.058e-31 Identity = 83/199 (41.71%), Postives = 116/199 (58.29%), Query Frame = 3
Query: 3141 LREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQLPFKITVGD------------YYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKKTFKQTLINITDNKDEDNQAVQTDLVNEDELN 3701
LR ++ +K + S+C GEAVGLLAAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPRLREILM+ S IKTP M VP+L A+ S+ K+ T++ L +++Q KI V + Y + F F Y+ + L+ ++++ E F + +++KK K + N + +A Q DL N EL
Sbjct: 1192 LRTLLQLKWQRSLCEPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKALKRVKSLKKQLTRVCLGEVLQ---KIDVQESFCMEEKQNKFQVYQLRFQFLPHAYYQQEKCLRPEDILRFMETRFFKLLMESIKK--KNNKASAFRNVN-TRRATQRDLDNAGELG 1384 HSP 3 Score: 124.405 bits (311), Expect = 5.467e-27 Identity = 62/169 (36.69%), Postives = 101/169 (59.76%), Query Frame = 3
Query: 4005 FDVKSILLNIFENCVISSVAGVEKALIFNSNKSKR-----FATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
FD+ S+++++ VI + G+ + L+ + +K TEG N ELFKY + DL + YSN+I ++ YGIEAA+ IE EIK VF Y I V+ RHL L+AD++ G NR + ++SSPLQ+ +FET+ + + G+ D ++SP++ ++ G++V
Sbjct: 1538 FDMSSLVVSLAHGAVIYATKGITRCLLNETTNNKNEKELVLNTEGINLPELFKYAEVLDLRRLYSNDIHAIANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIRSNSSPLQQMTFETSFQFLKQATMLGSHDELRSPSACLVVGKVV 1706
BLAST of DNA-directed RNA polymerase subunit vs. UniProt/SwissProt
Match: sp|O35134|RPA1_MOUSE (DNA-directed RNA polymerase I subunit RPA1 OS=Mus musculus OX=10090 GN=Polr1a PE=1 SV=2) HSP 1 Score: 575.474 bits (1482), Expect = 5.341e-176 Identity = 385/1102 (34.94%), Postives = 573/1102 (52.00%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQ---------------GLLESICDLGQIKDSLIKRFND------YENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMK-----------IDENPKIRDFKSSYSQEEL---YGKFTNTDTS-------IFPEILREHLRLLWSNES-------ENLQLILPMLKYNNFKYPTDVFFMENMLINP----PMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREII-------------------KRKDSTAA-EQYYQNIREG----------ILELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPM--VIGHNPLEVVENL----KFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW-----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYL-VYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLM----EFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKN-NLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQ-LMEKRYLKEPRNTKPVLSKVLAKKI 3077
L I F Y+ EE++ LS K IT+ ++ LG + NGLYD +LG + KE C C F +C+GH G I+ VYNP LFD L ++ C CH L + G L+++ +L +I ++ D E ++ T Q N L + + C F K+ H++AK+ P CK + I P KS EL + D + + P +EHL +W NE L I P +N +FF++ +++ P P+NRL GDQM + +T N+ V++ +R+++ K DS+ A ++ + + G + LQ VN+ +DS + + PG+RQ ++KK+GLFR ++MGKRV++A RSVI PD I +EIG+P+VF +KLTYP+P+ P N L Q V+NGP+V+PGA V + G+R + V V + L + T ++ RH +GD L++NRQPTLHRPS+QAH + + P+ K LR+HY+NCKAYNADFDGDEMN H+PQS L +AEA +L Q+ PKDG PLAGLIQDH+++G M +R FTR + ++LVY GL + R KL PAI+ P LWTGKQ++S +L N IP N+S + W R D + + ++II +G + G+ DK H G S GL E+YG E + ++LT L+RL T YL +Y GF+ ++D + + + R+ I+ + G +V + ++P E +G+ + + Q D ID FK ++Y +++ + C L +FP+NNL +M Q+GAKG N +I+ LG IE+EG+R PL G++LP F+ Y+ P + GF RF++G++P E FF MAGR+GLVDTAVKTS SGYLQRC+IKHLE + + +D TVRD+ +++QF YG DGLD+ + L +P +A N + +M+ ++L E VLS+ +K+
Sbjct: 12 LQGISFGMYSAEELKKLSVKSITNPRYVD-YLGNPSANGLYDLALGPADSKEVCATCVQDFNNCSGHLGHIDLPLTVYNPFLFDKLYLLLRGSCLSCHMLTCPRAAIYLLISQLRVLEVGALQAVYELERILSRFLEETGDPSAFEIQEELEEYTSKILQNNLLGSQGTHVKNVCESRSKLVAQFWKT-------HMAAKQC-----PHCKTGRSVVRKEHNSKLIITYPATVHKKSDQEGTELPEGVPEAPGIDKAQMGKRGYLTPSSAQEHLFAIWKNEGFFLNYLFSGLDDIGPESSFN-----PSMFFLDFIVVPPSRYRPVNRL-----GDQMFTNGQTVNLQAVMKDAVLIRKLLALMAQEQKLPCEMTELTIDKENDSSVAIDRSFLGLLPGPSLTDKLYNIWIRLQSHVNIVFDSEMDKLMLEKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSRTALSSVDAAQREAVAKQLLTPATGAPKPQGTKVVCRHVKNGDILLLNRQPTLHRPSIQAHRARIL--PEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYRGLTDKVGR-VKLFPPAILKPFPLWTGKQVVSTLLINIIPEDYAPLNLSGKAKIGSKAWVKEKPRPIPDFDPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGRVLTCLARLFTAYLQLYRGFTLGVEDILVKPNADVVRQRIIEESTQCGPQAVKAALSLPETASCDEIQGKWQDAHLSKDQRD--------FNMIDMKFKEEVNHYSNEINKACMPLGLHRQFPENNLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIRPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVIQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFL-QPKQFPFLAGNYEVIMKSKHLHE------VLSRADPQKV 1072 HSP 2 Score: 135.576 bits (340), Expect = 1.974e-30 Identity = 79/199 (39.70%), Postives = 117/199 (58.79%), Query Frame = 3
Query: 3051 LSKVLAKKID----ELVVKLKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQ---LPFKITVGD------YYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKK 3608
+S+ KKID E + +R + + S + LR ++ +K + S+C GEAVGLLAAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPRLREILM+ S IKTP M VP+ A+ S+ K+ T++ L +++Q + +G+ Y + F F Y+ + L+ ++++ E F + + +KK
Sbjct: 1166 VSETFEKKIDDFSQEWAAQAERSYKK-SELSLDRLRTLLQLKWQRSLCDPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVFDTKKALKKVKSLKKRLTRVCLGEVLQKVDIQESFCMGEKRNKFQVYELRFQFLPHAYYQQEKCLRPEDILHFMETRFFKLLMEAIKK 1363 HSP 3 Score: 127.872 bits (320), Expect = 4.742e-28 Identity = 66/180 (36.67%), Postives = 104/180 (57.78%), Query Frame = 3
Query: 3972 IKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNSNKSKR-----FATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
+ + L L FD+ S+++++ ++ + G+ + L+ + SK TEG N ELFKY I DL + YSN+I M+ YGIEAA+ IE EIK VF Y I V+ RHL L+AD++ G NR + + SSPLQ+ +FET+ + + G+ D +KSP++ ++ G++V
Sbjct: 1524 VTVKLPLMKINFDMSSLVVSLAHKAIVYTTKGITRCLLNETTNSKNEKELVLNTEGINLPELFKYSEILDLRRLYSNDIHAMANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIQSSSSPLQQMTFETSFQFLKQATMMGSHDELKSPSACLVVGKVV 1703
BLAST of DNA-directed RNA polymerase subunit vs. UniProt/SwissProt
Match: sp|P15398|RPA1_SCHPO (DNA-directed RNA polymerase I subunit rpa1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rpa1 PE=1 SV=2) HSP 1 Score: 572.007 bits (1473), Expect = 7.541e-175 Identity = 378/1060 (35.66%), Postives = 565/1060 (53.30%), Query Frame = 3
Query: 762 LREHLRLLWSNESENLQLILPMLKYNNFKYPT--DVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAA------------EQYYQNIREGILELQKRVNVCYDSGRHRELTGSQ----PGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVVEN--LKFQLQNNQTVIIN----------------RHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREF---KLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIG----STSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETC-KNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRY---------------------LKEPRNTKPVLSK--------------------VLAKKIDELVVKLKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINS--ATSVLKKFTKLYLKDI---IQLPFKIT------VGDYYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKKTFKQTLINITDNKDE 3653
+R HLR L+ E+ ++L L + P D+FF++N+ + PP PA GD++ E+ + + ++++ + + KDST + ++ + ++LQ VN DS R+ G PG++Q ++KK+GLFR ++MGKRVN+A RSVISPDP I +EIGVP VF +KLTYPEP+ N + + V+NGP +PGA I N +I PL + + L QL Q+ +I+ RH +GD LI+NRQPTLH+PSM AH + + P KT+RMHY+NC +YNADFDGDEMN H+PQS A++EA+ + N Q+ P G PL GLIQDH++ G + +D+ +TR+E L++ L + K PAI P WTGKQI+S VL N P+ N+ W +E + D EL+ C GI DK G S GL + ELYGP++A ++L+ LSRL T Y GF+ + D L+E G+ R+ +L +S G S VG S + P+ +L E + D+ K+ +D K + ++ C + L KFP N++ M +GAKG NV++I+ LG E+EG+R PL V G++LPSF Y+ S GF SRF++G+ PQE +F MAGR+GL+DTAVKTS SGYLQRCL+KHLE + V +D TVRD+ +I+QF YG D LDV L++ +F AKN + + ++Y LK+P PVL K K D+L+ K ++ S +E+ + ++ ++ + S+ GE+VG+LA+QS+GEPSTQMTLNTFH AG NVTLGIPRLREI+M S I+TP+M L +D ++ A++ K+ KL L ++ +++ KI+ Y I ++++Y+++ + ++E+ + F N F++ + +K ++ + KD+
Sbjct: 328 VRNHLRRLFVKEN----VVLSRLYAHKRGKPASADMFFLQNIAV-PPTRFRPASKMGDEVHENIQNELLTRILQSSIQIASL--SKDSTVEVNPDEKEGLERRSRAFELLINAFVQLQHDVNSLIDSNRNPSSGGQSRTVPPGIKQILEKKEGLFRKHMMGKRVNYAARSVISPDPNIETNEIGVPPVFATKLTYPEPVTLYNFNEMRNAVINGPHKWPGAS--HIQNEDGTLISLMPLTIEQRTALANQLLTPQSNLISSPYSYSRLINTNKKVYRHVRNGDMLILNRQPTLHKPSMMAHKARIL--PGEKTIRMHYANCNSYNADFDGDEMNMHFPQSTNARSEAQFIANTDSQYLVPTSGDPLRGLIQDHVVMGVWLTCKDTFYTRDEYQQLLFQALKPDETGMYGRIKTLPPAIQRPGIYWTGKQIISSVLLNLKPSDRPGLNLKSKAKVPGKYWSPDSEEGSVLFDDGELL------C-GILDKSSFGASAFGLVHSVHELYGPDIAGRLLSVLSRLFTAYAQMRGFTCRMDDLRLDEQGDNWRRQLLENGKSFGLEAASEYVGLSTDSPIALLNA----NLEEVYRDDE------KLQGLDAAMKGKMNGLTSSIINKCIPDGLLTKFPYNHMQTMTVSGAKGSNVNVSQISCLLGQQELEGRRVPLMVSGKSLPSFVPYETSAKSGGFIASRFLTGIAPQEYYFHCMAGREGLIDTAVKTSRSGYLQRCLMKHLEGLCVQYDHTVRDSDGSIVQFHYGEDSLDVTKQKHLTQFEFS---AKNYKSLIQKYKVKSVLSAVDSETASSYAKKALKKPYKYDPVLDKYPPSRYLGSVSEKFQRAVDEYTQKNPDKLIASKKESKLDDSLLNESKFKALMQLRYQQSLVDPGESVGVLASQSIGEPSTQMTLNTFHFAGFGAKNVTLGIPRLREIIMTASANIQTPTM---TLRLNDGVSDKRASAFCKEVNKLVLSEVVRQVRVTEKISGQGSDEQSKTYAIRLDLYSRDEYQDEYGVLQEEIESTFSNRFLKILNRIIKSYLAKSKQRKSGGKDD 1353 HSP 2 Score: 103.99 bits (258), Expect = 8.879e-21 Identity = 58/191 (30.37%), Postives = 103/191 (53.93%), Query Frame = 3
Query: 3966 DW--IKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNSNKS----KRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTNAM 4520
+W ++L +N ++ + S++ VI + G+ + + + + TEG N ++++ + +N Y+N+I + R+YG+EAA + I +E+ +VF Y I V+ RHL LIAD++T G AFNR + ++SP + SFET + E G VD + +P+S ++ G+ V N GT +
Sbjct: 1488 EWATVELVFPINTEKLLMVSLVEKACSETVIHEIPGITRCFSKPPDSALDTVPKVITEGVNLKAIWEFYNEISMNDIYTNDIAAILRIYGVEAARNAIVHEVSSVFGVYGIAVDPRHLSLIADYMTFEGGYKAFNRMGIEYNTSPFAKMSFETTCHFLTEAALRGDVDDLSNPSSRLVVGR-VGNFGTGSF 1677 HSP 3 Score: 63.1586 bits (152), Expect = 2.341e-8 Identity = 31/104 (29.81%), Postives = 50/104 (48.08%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQ 392
+ S+ F Y ++V +S K+I + + ++ L GLYD +LG C C+L C GHFG I Y+PL F + +++ C YCH ++
Sbjct: 11 IKSVKFGIYDVDDVEKISVKQIVNPVLLDN-LNHPTNGGLYDLALGPYLKNSVCATCHLDERYCPGHFGHIVLPIPAYHPLFFSQMYNLLRSTCLYCHHFKLSK 113
BLAST of DNA-directed RNA polymerase subunit vs. UniProt/SwissProt
Match: sp|O54889|RPA1_RAT (DNA-directed RNA polymerase I subunit RPA1 OS=Rattus norvegicus OX=10116 GN=Polr1a PE=1 SV=1) HSP 1 Score: 567.77 bits (1462), Expect = 3.940e-173 Identity = 399/1167 (34.19%), Postives = 597/1167 (51.16%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQ---------------GLLESICDLGQIKDSLIKRFND------YENMKSKTLSDKQKNALKIMSSQTR--------------------KNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYGKFTNTDTSIFPEILREHLRLLWSNES-------ENLQLILPMLKYNNFKYPTDVFFMENMLINP----PMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREII-------------------KRKDSTAA-EQYYQNIREG----------ILELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPM-VIGHNPLEVVENLKFQLQNNQTVI--------INRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW-----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYL-VYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLM----EFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKN-NLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQ-LMEKRYLKEPRNTKPVLSKVLAKKIDELVVKLKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGE-PSTQMTLNTFH 3263
L I F Y+ EE++ LS K IT+ ++ LG + +GLYD +LG + KE C C F +C+GH G I+ VYNPLLFD L ++ C CH L + G L+++ +L +I ++ +D E ++ T Q N L + + K C C V +K ++T+ A + G+ K E P+ + ++ GK + P +EHL +W NE L I P +N +FF++ +++ P P+NRL GDQM + +T N+ V++ +R+++ K DS+ A ++ + ++ G + LQ VN+ +DS + + PG+RQ ++KK+GLFR ++MGKRV++A RSVI PD I +EIG+P+VF +KLTYP+P+ P N L Q V+NGP+V+PGA V + G+R + + E V QL T I + RH +GD L++NRQPTLHRPS+QAH +A P+ K LR+HY+NCKAYNADFDGDEMN H+PQS L +AEA +L Q+ PKDG PLAGLIQDH+++G M +R FTR + ++LVY GL + R KL PAI+ P LWTGKQ++S +L N IP N++ + W R D + + ++II +G + G+ DK H G S GL E+YG E + ++LT L+RL T YL +Y GF+ ++D + + + R+ I+ + G +V + N+P E +G+ ++ Q D ID FK ++Y +++ + C L +FP+NNL +M Q+GAKG N +I+ LG IE+EG+R PL G++LP F+ Y+ P + GF RF++G++P E FF MAGR+GLVDTAVKTS SGYLQRC+IKHLE + + +D TVRD+ +++QF YG DGLD+ + L +P +A N + +M+ ++L E VLS+ +K+ +K+ S LLR+ + I +A+ L G P TQ L +H
Sbjct: 12 LQGISFGMYSAEELKKLSVKSITNPRYVDS-LGNPSADGLYDLALGPADSKEVCSTCVQDFNNCSGHLGHIDLPLTVYNPLLFDKLYLLLRGSCLNCHMLTCPRAAIHLLVCQLKVLDVGALQAVYELERILSRFLEETSDPSAFEIQEELEEYTSKILQNNLLGSQGAHVKNVCESRSKLVAHFWKTHMAAKRCPHCKTGRSVVRKEHNSKLTITYPAMVHKKSGQ------KDAELPEGAPAAPGIDEAQM-GK----RGYLTPSSAQEHLFAIWKNEGFFLNYLFSGLDDIGPESSFN-----PSMFFLDFIVVPPSRYRPINRL-----GDQMFTNGQTVNLQAVMKDAVLIRKLLAVMAQEQKLPCEMTEITIDKENDSSGAIDRSFLSLLPGQSLTDKLYNIWIRLQSHVNIVFDSDMDKLMLEKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSRTALSAVDATQREAVAK---QLLTPSTGIPKPQGAKVVCRHVKNGDILLLNRQPTLHRPSIQAH--RAHILPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYRGLTDKVGR-VKLFPPAILKPFPLWTGKQVVSTLLINIIPEDYTPLNLTGKAKIGSKAWVKEKPRPVPDFDPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGRVLTCLARLFTAYLQLYRGFTLGVEDILVKPNADVMRQRIIEESTQCGPRAVRAALNLPEAASCDEIQGKWQDAIWRKDQRD--------FNMIDMKFKEEVNHYSNEINKACMPFGLHRQFPENNLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIRPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVIQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFL-QPKQFPFLASNYEVIMKSKHLHE------VLSRADPQKVLRHFRAIKKWHHRHS---SALLRKGAFLSFSQKIQAAVKALNLEGKTQNGRSPETQQMLQMWH 1132 HSP 2 Score: 133.65 bits (335), Expect = 7.262e-30 Identity = 77/198 (38.89%), Postives = 115/198 (58.08%), Query Frame = 3
Query: 3051 LSKVLAKKIDELVVKLKRDFVEISPKDE---NLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQ---LPFKITVGD------YYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKK 3608
+S+ KKID+ + + + E + LR ++ +K + S+C GEAVGLLAAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPRLREILM+ S IKTP M VP+ A+ S+ K+ T++ L +++Q + +G+ Y + F F Y+ + L+ ++++ E F + + +KK
Sbjct: 1166 VSETFEKKIDDYSQEWAAQAEKSHNRSELSLDRLRTLLQLKWQRSLCDPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVFNTKKALRRVKSLKKQLTRVCLGEVLQKVDIQESFCMGEKQNKFRVYELRFQFLPHAYYQQEKCLRPEDILHFMETRFFKLLMEAIKK 1363 HSP 3 Score: 129.798 bits (325), Expect = 1.097e-28 Identity = 67/180 (37.22%), Postives = 106/180 (58.89%), Query Frame = 3
Query: 3972 IKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALI---FNSNKSKRFA--TEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
+ + L L FD+ S+++++ N ++ + G+ + L+ NS K F TEG N ELFKY + DL + YSN+I ++ YGIEAA+ IE EIK VF Y I V+ RHL L+AD++ G NR + + SSPLQ+ +FET+ + + G+ D +KSP++ ++ G++V
Sbjct: 1523 VTVKLPLMKINFDMSSLVVSLAHNAIVYTTKGITRCLLNETINSKNEKEFVLNTEGINLPELFKYSEVLDLRRLYSNDIHAVANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIQSSSSPLQQMTFETSFQFLKQATMMGSHDELKSPSACLVVGKVV 1702
BLAST of DNA-directed RNA polymerase subunit vs. TrEMBL
Match: A0A0X3PF39 (DNA-directed RNA polymerase subunit OS=Schistocephalus solidus OX=70667 GN=TR167060 PE=3 SV=1) HSP 1 Score: 817.379 bits (2110), Expect = 0.000e+0 Identity = 501/1337 (37.47%), Postives = 716/1337 (53.55%), Query Frame = 3
Query: 72 DCNLDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIK---RFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVH-------------------------------ISAKESRDKGEPSCKRM--KIDENPKI---RDFK--SSYSQEELYGKFTNTDTSIF--PEILREHLRLLWSNESENLQLILPMLKY--NNFKYPTDVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREII--------------------KRKDSTA---------------------AEQYYQNIREGILELQKRVNVCYDSGRHRELTGSQ---------PGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVVENLKF-----------QLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLP-----KPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNT---MWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQF-DKSDK-FRNKMTEIDTNFKT-LSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRY--------LKEPRNTKPVLSKVLAKKIDELVVK----------------------LKRDFVEISPKDE---------NLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQLPFKITV---GDYYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLK 3605
D ++S FSFY+PEE++ LS EITS + + +G GL+D +LG ++++ C +C L + C GHFG I F+K YNPL FD++ + +K+ CF C ++ L ++ LG + D L+ + ND E ++S + + +K A++ MS + R C C ++ + + +I + +A E++ G+ S + +E I DF+ S +++L N F PE++R+ R LW E+ L++++P L +YPTD+FF+E M I+P R P + G E P T +++ ALR+I+ + TA AE+ + I+ LQ +N YD+ G + PGL+Q+++KK GLFR ++MGKRVN+ACRSVISPDP + + E+GVP+ ++LT+PEP+ +N D L + V GPD YPGA S Q + +++ + E+ + L N I+NRH GD L+MNRQPTLHRPSMQAH ++ I P AKTLR+HY+ CKAYNADFDGDEMNGH+PQ++ A+AE + V YQ+ PKDGSPL GLIQDH+IA K+ +RD F R E LDLVY L+ S LP KPAI++P +LWTGKQ++S +L N P G LP+I+ K T +W + + LSDV+L+I GY+ +G+ +K H+G + GL + E YGP A ++L+ +SR+ R+L F+ S+ D L E + R+ +++ +G + +F VP E E+ L A F +SD+ + K+ +D K L ++ + L FP N+L LM GAKGG N ++++ALG IE+EG+R PL + GRTLPSF YD+ P + G C RF++ + PQELFF SMAGRDGLVDTA KTS SGYLQR LIKHLED++V +DGTVRD+ IIQFRYG DGLDV S S K + A N +L+ +R+ K + P L+ LA ++DE+ VK L + + P+D LR I+ K P G+ VGLLAAQSVGEPSTQMTLNTFH AG+ EMNVTLGIPRLRE+LM GS I TP M+VPIL A + + F KL L ++++LP I V D +TF + + Y+ +T++K +I FE F+ + AK L+
Sbjct: 6 DGQINSYGFSFYSPEEIKKLSVIEITSNATFDKFTNRGIDGGLHDMALGPCSERDACGHCGLDYVQCPGHFGHISFAKPAYNPLFFDLVVRLLKSFCFSCFKICNVDCLSAALQLLG-LPDILLPSKPKPNDLEELRSCSPFELRKAAIRAMSMRQRLPCRNCSERAWISRHINHQQIVMRPVSDLRNSRYTANSKSKFDGDADHEPGELECWAAIEAQLLGDKSTSKTVTTFEEGSAIYAGMDFQDLSEAVRDQLKRLADNKQKESFAQPELIRDMFRCLWMQEASLLRILMPCLHVASTKMRYPTDIFFLEVMPISPSFCRWPRFVGG-YAYEHPTTAIFSRIIKRAIALRQIVDYMEEMNLTGNNLPVKASQLRATSKTALDDVTPDVIGHRIQLANVPMTAERACTALHVSIVFLQAAINAVYDATTSTLPVGFENRDKSITILPGLKQRLEKKQGLFRMHMMGKRVNYACRSVISPDPNLRVHEVGVPLFIATQLTFPEPVTALNVDRLRKLVTAGPDNYPGARSIQTPDGVKLILPGSKDEIHRKRRLNLAQRLVPPQPDLHNGAPTIVNRHLQDGDLLVMNRQPTLHRPSMQAHRVRIIRSPGAKTLRLHYAICKAYNADFDGDEMNGHFPQTIQAKAELAHIAAVPYQYLAPKDGSPLGGLIQDHVIAAVKLTIRDRFFDREEYLDLVYSALYASIGEAGSLPDVILLKPAILWPTRLWTGKQVVSTLLMNITPPG--LPHINAHIEGKRTKIELWAGAPFARAKLLSDVDLVICGGYLVSGMLEKAHIGSAAGGLVHFVHEAYGPHAASQLLSGISRMSDRFLKIVSFTMSLADIALREKADRDRRRRFRRLQDLGLCAFADAFGVPHGELSEERVKCLYRRAHFAAQSDEVYGGKLAALDCAMKERLKASQDAVCDAAIPSGLYVPFPSNSLQLMVHIGAKGGMVNAQQMSVALGQIELEGRRVPLMLSGRTLPSFPPYDIRPRAGGMCTHRFLTSMPPQELFFHSMAGRDGLVDTACKTSRSGYLQRSLIKHLEDLSVQYDGTVRDSGGGIIQFRYGDDGLDVCQS-SFLKSTGIHFFADNAELLSERWKCPDDPDLCKRMIDANP-LAPSLAHEVDEVAVKRMGVLQKSVSENKLASIRRRRQLAAERLAAVPRDSCSEAPCRLAEDLRTIVTAKIIQGQAPPGDPVGLLAAQSVGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREVLMSGSAVISTPCMEVPILPTAAARTKVEHIARSFYKLRLTEVVKLPLGIGVDYGSDTCTLTFHLQPPSAYEARTNVKPSRIIRFFERVFLPSLAKRLE 1336 HSP 2 Score: 152.91 bits (385), Expect = 3.313e-33 Identity = 75/201 (37.31%), Postives = 120/201 (59.70%), Query Frame = 3
Query: 3930 FHNYVIESKPEPDWIKLYLNLNHKR-----FDVKSILLNIFENCVISSVAGVEKALIFNSN-KSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTN 4514
F +Y ++ P W+++ + + + D+++++ I V+S+V G+ KA + S+ K+ F EG N +EL +Y +F LNK Y+NN++ M + YGIEAA +C++ E+ VF Y I VN RHL L+ D+LT +G AFNR M H SP+QR +FETA V+ ++ V+ + SP+ +++ GQLV GT
Sbjct: 1554 FSDYAYDTHRSPSWVQIKICMFDPKDGRISTDLRTLVQRILNASVVSAVPGITKAFVDKSDPKNWVFRIEGINVMELMRYPDVFALNKLYTNNVLLMQQHYGIEAARACLQREVSGVFGHYGIYVNPRHLGLVTDYLTKTGIYRAFNRRTMDFHPSPMQRVTFETATGVLKTVIQEDLVETMVSPSGSLIPGQLVHGYGTT 1754
BLAST of DNA-directed RNA polymerase subunit vs. TrEMBL
Match: A0A4E0RIY1 (DNA-directed RNA polymerase subunit OS=Fasciola hepatica OX=6192 GN=D915_002719 PE=3 SV=1) HSP 1 Score: 809.675 bits (2090), Expect = 0.000e+0 Identity = 492/1348 (36.50%), Postives = 712/1348 (52.82%), Query Frame = 3
Query: 60 MKNYDCNLDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLI--KRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEI---TVHISAKESRDKGE----------------------PSC------------------KRMKIDENPKIRDFKSSYSQEELYGKFTNTDTSIFPEILREHLRLLWSNESENLQLILPML--KYNNFKYPTDVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREIIK---RKDSTAAEQYYQNIR------------------EGILEL-----QKRVNVCYDSGRHRELTGSQ-----------PGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVVENLKFQLQNNQT----------VIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLH---NSNQREFKLP------KPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPK-KNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKS--DKFRNKMTEIDTNFKTLSSNYIHQLVETC-KNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNTKPVLSKVLA-----------------------------KKIDELVVKLKRD---FVEISPKDENL--------------------LREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQLPFKITV---GDYYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKKTFK 3617
M + ++S+ F FYT +EVR+LS ++IT+ ++ + + G+G +GL+D SLG ++K+ C +C L F+ C GHFG IEF+K V+NP+ FD+L K +++ CF C L L ++ LG +L + D ++ T S+ + L++++++ R C C +++ V + + K ++ V +A++ K E P C K ++I++N + + L+ + + + + PE R LR LW N++ +L L++PML KY TD+FFME +LI+P + RLP + G E PET VV+ L +I++ ++ ++A Q+I EG L+L Q VN +DS TG + PGLRQ+++KK+GLFR ++MGKRVN+ACRSVISPDP + + E+GVP+ F KLT+P P+ N D L Q V+NGPD YPGA S Q + +++ + + + T I++RH + GD L+MNRQPTLHRPSMQAH ++ I+ AKTLRMHYSNC+AYNADFDGDEMNGH QS QAE ++L V + + PKDGSPLAGLIQDH+IA K+ +RD F ++ +DLVYH + N ++R P PAI++P +LWTGKQ++S +L N P GL N + + K +W + Q LSDV++++ DGY+ +G+ DK H+G S GL + E YGPE A +L ++ L L +T F+ IKD L+ + R ++ +G + +F + + K L A F S +M ++D K + ++ N L FP+NNL LM GAKGG N ++++ALG IE+EG+R PL + GR+LPSF YD+ P + G C RF++ L PQELFF SMAGRDGLVDTAVKTS SGYLQR IKHLED+++ +DGTVRD+ NIIQFRYG DG+DV S L + + + A N L R+ P ++++ +KI L+ K + F EI+ K + L LR++ K + P G+ VGLLAAQSVGEPSTQMTLNTFH AG+ EMNVTLGIPRLREILM GS + TP M+VP+ A S + K+ KL L + IQLP V D + F ++ Y ++ +K ++ E A+ L + +
Sbjct: 1 MSCSEGQIESLQFRFYTADEVRSLSVRQITNELAFDKLTGRGVDSGLHDVSLGLNSEKDLCGHCGLDFFSCPGHFGHIEFAKPVFNPMFFDLLYKLMRSFCFGCFHLYTVDYLAAALHYLGAPAYALPGKPKQTDLSELEGVTPSELRALGLRVLAARPRTPCKLCCSRAWVLRHANKQQLLLRPVSENARQRSTKAEAAQNEDEEGVNILLEMEDPRLMPHCSTSTDETKHMFVDTAFVDKELEIEQNAMLESVRGEIQNMLLFCRGSK-EACLLPETARRMLRCLWMNDAISLSLLIPMLCSASKQHKYATDIFFMETVLISPSLCRLPRYVGG-LAYEHPETAVYSRVVKRAQCLAQIVQFINQQSASADSNPEQSINADVSALSNLVDGNSTGRAEGALKLASLLLQAAVNGLFDSNTATIPTGLESKSGVGAGVRLPGLRQRLEKKEGLFRMHMMGKRVNYACRSVISPDPNLDVTEVGVPLFFARKLTFPVPVTKFNLDRLRQLVINGPDKYPGARSLQTPDGRLLLLAPGTSDQSVRRRTVVARRLTPLSADSTGIPYIVHRHLMEGDMLLMNRQPTLHRPSMQAHRVRIIYSSGAKTLRMHYSNCRAYNADFDGDEMNGHLVQSYAGQAEQEILAAVPHHYLVPKDGSPLAGLIQDHVIAAVKLTMRDRFFDHSDYMDLVYHSVRSVFNVSRRSGNGPPNLITLPPAIMWPKKLWTGKQVISTLLMNLTPVGLPFLNATLRGRRTKAELWSGAAPGQPTPLSDVDVVVCDGYLASGMLDKAHIGSSTGGLVHCVYEAYGPESAAHLLNGINLLANELLKFTAFTMGIKDMLLSTEADADRTRRFENLKDLGLCAFADAFELTTDQLTERKVRQLYRKAHFAPSTDQTLCKRMAQLDLAMKNRLKRAQDAVCDSAIPNGLYVGFPENNLQLMVHVGAKGGMVNAQQMSVALGQIELEGRRVPLMLSGRSLPSFPPYDVRPRAGGMCTHRFLTSLPPQELFFHSMAGRDGLVDTAVKTSRSGYLQRSAIKHLEDLSIQYDGTVRDSGKNIIQFRYGDDGIDVCQSSFLQQAG-LNLFADNAALFNHRWHSSTAEEDPARARLIEQNPLPRPLQMVADQVGVQRQMLIHESMPHRKISRLIRKAHQAKVVFDEIAEKQKQLSEYELLNDTLPPPPCSAAAELRQLCVAKLISAQAPPGDPVGLLAAQSVGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMSGSSQTSTPCMEVPVRPSAAAHRSCMQIAKRMYKLKLVEAIQLPISERVDYASDSCTLHFQLLPRSAYAERSLIKPARVLRFLERVLFSDLARRLDRELR 1345 HSP 2 Score: 155.606 bits (392), Expect = 4.982e-34 Identity = 80/218 (36.70%), Postives = 126/218 (57.80%), Query Frame = 3
Query: 3879 EYISKTKANVKAMLPGVFHNYVIESKPEPDWIKLYLNLNHKR-----FDVKSILLNIFENCVISSVAGVEKALIFNSNKSK-RFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTN 4514
E ++++ L F Y + P W +L + + + D+K+++ I V+SSV G+EK ++ SN+S+ + EG N +E+ +Y +FDL++ Y+NNI M++ YGIEAA + I+ E+ +VF Y I VN RHL LIAD++T SG AFNR H SPLQ+ SFET + + + D + SP+++++ G+LV N GTN
Sbjct: 1545 ELEAESRCTFVTRLHARFSRYDFDRSKTPTWARLTICMFDPKEGRISIDLKTLVTRIVNRSVVSSVPGIEKVIVDKSNQSEWMYRVEGVNLIEMMRYPDVFDLSRLYTNNICVMNQTYGIEAARAAIQKEVSSVFGHYGIYVNPRHLSLIADYMTKSGCYRAFNRMSFRAHPSPLQQMSFETVTSALKATIQDDRTDPLVSPSASLVVGRLVQNCGTN 1762
BLAST of DNA-directed RNA polymerase subunit vs. TrEMBL
Match: A0A5K3EH83 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1) HSP 1 Score: 801.201 bits (2068), Expect = 0.000e+0 Identity = 503/1352 (37.20%), Postives = 713/1352 (52.74%), Query Frame = 3
Query: 60 MKNYDCNLDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIK---RFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVH--ISAKESR----------------DKGEPSCKRM--------KIDENPKIRDFKSSYSQ--------------EELYGKFTNTDTSIF--PEILREHLRLLWSNESENLQLILPMLKYNN--FKYPTDVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREI---IKRKDSTA------------------------------------AEQYYQNIREGILELQKRVNVCYD----------SGRHRELTGSQ-PGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVI--GHNPLEVVENLKFQ---------LQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLP-----KPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNT---MWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKS--DKFRNKMTEIDTNFKTLSSNYIHQLVETCKNN----LREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKR---------YLKEPRNTKPVLSKVLAKKIDELVV-----------------------KLKRDFVEISPKD---------ENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQLPFKITV---GDYYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKKTFK 3617
M+ D + + FYTP+E+R+LS EI+S + + +G GL+D +LG N+K+ C +C L + C GH G IEF K VYNPLLFD++ + +K+ CF C R+ Y LL + +L + D L+ + ++ E+++S ++ +K A++ MS++ R C C +K+ + K +I + ++ SR + GE C K P + F+ S SQ E L + F PE++R+ LR +W +S L++I P L + F Y TDV FME M ++P R P ++NG E P T V+ ALR+I +K D AE+ + ++ LQ VN YD R R T PGL+Q+++KK GLFR ++MGKRVN+ACRSVISPDP + + E+GVP+ KLT+PEP+ + L + V GPDVYPGA+ Q+ + +I G++ V L + + +I++RH GD L+MNRQPTLHRPS+QAH ++ I P AKTLR+HY+ CKAYNADFDGDEMNGH+PQ+ A++E L V YQ+ PKDGSPL GLIQDH+IA K+ RD F ++E LDLVY+ L+ S + LP PAI+ P++LWTGKQ++S +L N PAG LP ++ + T +W + LSDV+L+++ GY+ +G+ DK H+G + GL E YG A ++L+ +SR+ R+L F+ S+ D LNE + R+ K++ +G + +F + EK +L A F + + + K+ +D + + H C ++ L FP N+L LM GAKGG N ++++ALG IE+EG+R PL + GRTLPSF YD+ P + G C RF++ + PQELFF SMAGRDGL+DTA KTS SGYLQR LIKHLED++V +DGTVRD+ +IQFRYG DGLDV S L KP + A+N +L+ +R Y K N LS LA+K+DE+ V + +R E K LR I+ K P GE VGLLAAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPRLRE+LM GS I TP M+VPIL A A + + KL L ++I+LP + V D +TF + Y+ +T+++ + +I FE +K L + K
Sbjct: 1 MELTDGRISEFGYCFYTPQEIRSLSVLEISSDATFDRFTNRGIDGGLHDMALGPCNEKDICAHCGLGYVQCPGHMGHIEFFKPVYNPLLFDLVVRLLKSFCFSCFRI-YNVDLLSACLELLGLPDILLPSKPKPSEIEDLRSLPSAELRKTAIRAMSTRPRIPCRNCSSKAWIPKHINHQQIVMRPVSDSRNSRFTGVNKYNVGDGDTEHEPGELECWAAIEAQLLCDKATSRPVV-SFEDSTSQTTRIEFDDIAESVRERLKEISSGQQKEAFSSPELVRDMLRCVWLQDSSILRIIFPFLHAASAKFHYATDVCFMEVMPVSPSFCRWPRMVNG-MAYEHPATALFSRVINRAIALRQIAEYLKENDLVGPQGLSKTSDVVSAAKQAQLSTVDGAAGHKISPTLMTAERATAALHASVIFLQAAVNAVYDVNISVLPTGFESRTRNATTVPLPGLKQRLEKKQGLFRMHMMGKRVNYACRSVISPDPNLRVFEVGVPLFIALKLTFPEPVTAHSIGKLRRLVRTGPDVYPGAIVVQMPDGSKQIIPPGNSEPAVARRLTMSKRLAIPQTGVHDGMPMIVHRHLEDGDYLVMNRQPTLHRPSIQAHRVRIIQAPGAKTLRLHYAICKAYNADFDGDEMNGHFPQTYQAKSEMTQLAAVPYQYLAPKDGSPLGGLIQDHVIAAVKLTTRDRFFDQDEYLDLVYNALYASVAEDGCLPDVILLPPAILKPSRLWTGKQVVSTLLMNVAPAG--LPQLNAHIEGRRTKVELWAGVPFSRARILSDVDLVVVGGYLASGLLDKAHIGSASGGLVHCFYEAYGACAASQLLSGISRMADRFLKIASFTMSLADIMLNEKADKDRRKRFRKMQDLGLCAFADAFGLTADSLTEEKVQTLYRRAHFASTTDEIYGGKLAALD---RAVKDRIKHSQDAVCDSSIPSGLYTPFPANSLQLMVHVGAKGGMVNAQQMSVALGQIELEGRRVPLMLSGRTLPSFPPYDVRPRAGGMCTHRFLTSMPPQELFFHSMAGRDGLIDTACKTSRSGYLQRSLIKHLEDLSVQYDGTVRDSGGGVIQFRYGDDGLDVCQSAFL-KPAGINFFAENAELLTERWRCPNDPEVYQKMAENNP--LSPDLAQKVDEVAVNRLNVLQMSLSTKKLAHLQKRRERAQRKLTEADYKTGLPDPPCTLAGDLRTIVTAKVIQGQVPPGEPVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREVLMAGSSVIATPCMEVPILPNPVAKAKAEHIARGMYKLRLTEVIKLPLGVEVDYSSDSCSLTFNLLPLSAYEARTNVRPQRIIRFFERVLFVDLSKRLAQELK 1341 HSP 2 Score: 140.969 bits (354), Expect = 1.491e-29 Identity = 71/201 (35.32%), Postives = 116/201 (57.71%), Query Frame = 3
Query: 3930 FHNYVIESKPEPDWIKLYLNLNHKR-----FDVKSILLNIFENCVISSVAGVEKALIFNSNKSKR-FATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTN 4514
F +Y +++ P W+KL +++ + D+++++ I V+SSV + +A I ++ K EG N EL++Y +FDL + Y+N++ M +G+EAA + ++ EI VF Y I VN RHL L+AD+LT +G +FNR M H SP+QR +FETA +V+ ++ V+ SP+ +I+ GQL GT
Sbjct: 1543 FTDYSYDTQQPPSWVKLTISMFDPKDGRISIDLRTLVQRIVGRSVVSSVPNIRQAFIDKTDPLKWILRVEGVNVPELYRYGDVFDLTQLYTNHVHVMQEHFGVEAARASLQREISGVFDHYGIYVNPRHLGLVADYLTKTGLYRSFNRRTMDFHPSPMQRMTFETATSVLKSVIQEDLVESTVSPSGSIVPGQLTHGYGTT 1743
BLAST of DNA-directed RNA polymerase subunit vs. TrEMBL
Match: A0A094ZJ95 (DNA-directed RNA polymerase subunit OS=Schistosoma haematobium OX=6185 GN=MS3_01129 PE=3 SV=1) HSP 1 Score: 790.03 bits (2039), Expect = 0.000e+0 Identity = 505/1371 (36.83%), Postives = 723/1371 (52.74%), Query Frame = 3
Query: 96 FSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLS--DKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVH-IS-AKESRDKG------EPSCKRM----KIDENP---------KIRDFKSSYSQEELYGKFTNTDTSIF----------------PEILREHLRLLWSNESENLQLILPMLKYN--NFKYPTDVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREIIK------------------RKDSTAAE-------QYYQNIREGILELQKRVNVCYDSGRHRELTGSQ------------PGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVV----ENLKFQLQ------NNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLH---------NSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISC-SEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKS--DKFRNKMTEIDTNFKTLSSNYIHQLVETC-KNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNT---------------KPV--------------LSKVLAK----------KIDELVVKLKRDFVEISPKDENL--------------LREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQLPF--KIT-VGDYYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFA----KTLKKTFKQTLI------------NITDNKDEDNQAVQTDLVNE 3689
+ FY+P+E++ LS +++T+ ++ + G+G GL+D + G + E C +C + F DC GH G IEFSK V+NP +FD+L K +K+ CF C RL + L ++ LG L + E + + LS D ++ A++ ++++ R C C + S + K ++ +H IS A +R K E C + K+D+ + D E +Y +T ++ PE++R LR LW+++ + L L+ PMLK N +PTDVFFM+N+L++P + R P + G+ + E PET +V+ L I++ ST ++ Q ++ + LQ VN YD G + PGLRQ++++K+GLFR ++MGKRVNFACRSVISPDP + + E+GVP+ F +LT+P P+ N L Q V+NGP+ YPGA S Q N ++ N E L +L+ +N +++NRH L GD LIMNRQPTLHRPSMQAH ++ I AKTLR+HYSNCKAYNADFDGDEMNGH+ QS A+AE + L +V +Q+ PKDG+PLAGLIQDH+IA K+ +RD F + LDLVYH L +S KPAI +P LWTGKQ++S +L N P+GL N + K +W + LSDV+L+I DGY+ +G+ DK H+G S GL + E YGPE A +L +S L R+L + F+ +KD L+ + R+ + ++ +G + +F++ E L AQF S + ++ ++D K + ++ L FP+NNL LM GAKGG N ++++ALG IE+EG+R PL + G+TLPSF YD+ P + G C +RF++ L QELFF SMAGRDGLVDTAVKTS SGYLQR IKHLED+++ +DGTVRD+ NIIQFRYG DG+DV + L +P + A N+ LM+ R+ + NT KP+ L K ++K K+ +KL + S KD+ + LR++ K + P G+ VGLLAAQSVGEPSTQMTLNTFH AG+ EMNVTLGIPRLREILM GS I TP M+VPI +A + + KKF KL L + ++LP K+ V D F + K+ Y +++ + ++ E A + LK K +LI NK+ + A D+VN+
Sbjct: 14 YGFYSPDEIKRLSVRKVTNDLAFDKFTGRGVDGGLHDVNFGVIGYSETCAHCGMDFTDCPGHCGHIEFSKPVFNPFMFDVLYKIVKSFCFGCFRLYSAEYLASALYLLGAPVSKLPGKLKALEIKELEGLSGDDLRQVAIRNLATRPRAPCKLCGSGSWGLRHISKQQLVLHPISIAGGNRSKARMKEELEEDCSDLLEGCKVDDQRMEQKLSMFIDMSDGSEVSKAETIYTANLSTARNMLSEAIKSQQHSKEICFPPEMIRIMLRCLWAHDGDLLVLLFPMLKAARLNHTFPTDVFFMDNVLLSPSLCRWPRHV-GNLVYEHPETAVYVRIVKRAQCLHNIVEFIRQRNEVSNSADVNKPPEPKSTTSDGDLIDDAQSDGALKLATVLLQAAVNGIYDMSMAITPLGLESRSGISSNAVRLPGLRQRLERKEGLFRMHMMGKRVNFACRSVISPDPNLSVTEVGVPLFFAFRLTFPTPVTKFNLVHLRQLVMNGPNNYPGATSIQFSNGIITMLPPNDTPQAKKKREMLALRLKPLISGKDNIPIVVNRHLLDGDMLIMNRQPTLHRPSMQAHRVRIIKCSGAKTLRLHYSNCKAYNADFDGDEMNGHFVQSYAAKAELETLASVPHQYLVPKDGTPLAGLIQDHVIAAVKLTMRDRFFEHADYLDLVYHALRPLFAINRQTSSGPPSIITVKPAICWPKCLWTGKQVISTLLINLTPSGLLPLNATLLGRRTKAELWSGVAPGEPTPLSDVDLVICDGYLASGMLDKSHIGSSSTGLIHCVYEAYGPESASCLLNGISLLANRFLKFIAFTMGLKDILLSHRADEERQRRFSNLKDLGLCAFADTFSLKPDELTEHNVKRLYRHAQFPSSTDQSSQKRLAQLDMAIKDRLKRAQDAVCDSAIPGGLYVGFPENNLQLMVHVGAKGGMVNAQQMSVALGQIELEGRRVPLMLSGKTLPSFPPYDVRPRAGGMCTARFLTSLPAQELFFHSMAGRDGLVDTAVKTSRSGYLQRSAIKHLEDLSISYDGTVRDSGSNIIQFRYGDDGVDVCQAGFL-QPIGLHFFADNVNLMKMRWHSDKINTDNPAYAHLFEKNTLPKPIQIMADQVGVKRQCILHKTVSKHKLVKLLNSSKMAHKALKLMETEEKASGKDKIMNEIIPEPPCATAAELRDLCLAKIINAQAPPGDPVGLLAAQSVGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMSGSSNISTPCMEVPIWPTKEAQQLSEQIAKKFYKLKLSETLKLPIGQKVNYVSDSCTFEFNLQPKSVYSDQSHVTPVRVLRFLERILFPDLAHRLNEELKDQGKTSLIKSFALSAMARQQQAGQNKNSEEPAEDADIVNQ 1382 HSP 2 Score: 145.976 bits (367), Expect = 4.043e-31 Identity = 82/226 (36.28%), Postives = 129/226 (57.08%), Query Frame = 3
Query: 3870 EQSEYISKTKANVK----AMLPGVFHNYVIESK-PEPDWIKLYL-----NLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNSNKSKR-FATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTN 4514
EQ IS +A + L F Y + K P W +L + N D++++++ + V+SSV+G+EK +I S ++ F EG N +E+ +Y +FDL++ Y+NNI M+ YGIEAA + ++ E+ VF Y I VNSRHL LI+D+LT G AFNR M++H S LQ+ +FET V+ L ++D + SP+++++ G++V GTN
Sbjct: 1539 EQENAISHDEARKRITFVTSLHARFSGYDFDRKNSSPKWARLTICMFDPNEGRISIDLETLIMRLINRSVVSSVSGIEKTIIDRSKPNQWMFRVEGINMIEMMRYPQVFDLSRLYTNNITVMNETYGIEAARAALQREVSGVFGHYGIHVNSRHLSLISDYLTKFGVYQAFNRRSMSSHPSLLQQMTFETVANVLKNSLQNDSMDNLISPSASLVTGRIVHYAGTN 1764
BLAST of DNA-directed RNA polymerase subunit vs. TrEMBL
Match: G4VH38 (DNA-directed RNA polymerase subunit OS=Schistosoma mansoni OX=6183 GN=Smp_129500 PE=3 SV=1) HSP 1 Score: 786.178 bits (2029), Expect = 0.000e+0 Identity = 504/1371 (36.76%), Postives = 721/1371 (52.59%), Query Frame = 3
Query: 96 FSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLS--DKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHI--------SAKESRDKGEPSCKRM----KIDENP---------KIRDFKSSYSQEELYGKFTNTDTSIF----------------PEILREHLRLLWSNESENLQLILPMLKYN--NFKYPTDVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREIIK-------------------RKDSTAAEQYYQNIR-EGILEL-----QKRVNVCYDSGRHRELTGSQ------------PGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVV----ENLKFQLQ------NNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLH---------NSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNIS-CSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRN--KMTEIDTNFKTLSSNYIHQLVETC-KNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNT----------KPVLSKVLAKKIDELVVK-------------LKRDF----------------VEISPKDENL--------------LREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQLPF--KIT-VGDYYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFA----KTLKKTFKQTLI------------NITDNKDEDNQAVQTDLVNE 3689
+ FY+ +EVR LS +++T+ ++ + G+G GL+D + G + E C +C + F DC GH G IEFSK V+NP +FD+L K +K+ CF C RL + L ++ LG L + E + + LS D ++ A++ ++++ R C C + S + K ++ H S +++ E C + K+D+ + D + E +Y +T ++ PE++R LR LW+++ + L L+ PML+ N +PTDVFFM+N+L++P + R P + G+ + E PET +V+ L I++ K +T+ + + +G L+L Q VN YD G + PGLRQ++++K+GLFR ++MGKRVNFACRSVISPDP + + E+GVP+ F +LT+P P+ N L Q V+NGP+ YPGA S Q N ++ N E L +L+ +N +++NRH L GD LIMNRQPTLHRPSMQAH ++ I AKTLR+HYSNCKAYNADFDGDEMNGH+ QS A+AE + L +V +Q+ PKDG+PLAGLIQDH+IA K+ +RD F + LDLVYH L +S KPAI +P LWTGKQ++S +L N P GL N + K +W LSDV+L+I DGY+ +G+ DK H+G S GL + E YGPE A +L +S L R+L + F+ +KD L+ + R+ + ++ +G + +F++ E L AQF S + ++ ++D K + ++ L FP+NNL LM GAKGG N ++++ALG IE+EG+R PL + G+TLPSF YD+ P + G C +RF++ L QELFF SMAGRDGLVDTAVKTS SGYLQR IKHLED+++ +DGTVRD+ NIIQFRYG DG+DV + L +P + A NI LM+ R+ + NT K +L K + D++ VK L + F ++S KD+ + LR++ K + P G+ VGLLAAQSVGEPSTQMTLNTFH AG+ EMNVTLGIPRLREILM GS I TP M+VPI +A + + KKF KL L + +QLP K+ V D F + K+ Y +++ + ++ E A + LK K +LI NK+ + A D+VN+
Sbjct: 14 YGFYSSDEVRRLSVRKVTNDLAFDKFTGRGVDGGLHDVNFGVIGYSETCAHCGMDFTDCPGHCGHIEFSKPVFNPFMFDVLYKIVKSFCFGCFRLYSAEYLASALYLLGAPVGKLPGKLKTLEIKELEGLSGDDLRQVAIRNLATRPRAPCKLCGSGSWGLRHISKQQLVFHPISIVGSNRSKARMKEELEEDCSDLLEGCKVDDQRMEQNLSLFIDMSDGSEASKAEAVYTANLSTARNMLSEAIKSQQHSKEICFPPEMIRVMLRCLWAHDGDLLVLLFPMLQAARLNHTFPTDVFFMDNVLLSPSLCRWPRHV-GNLVYEHPETAVYVRIVKRAQCLHNIVEFIRQHNEVSNSVDVNKPVEPKSTTSNGDLIDDAQSDGALKLATVLLQAAVNGIYDMNMAITPLGLESRNGISSNSVRLPGLRQRLERKEGLFRMHMMGKRVNFACRSVISPDPNLSVTEVGVPLFFAFRLTFPTPVTKFNLVHLRQLVMNGPNNYPGATSIQFSNGVITMLPQNDTPQAKKKREMLALRLKPLISGKDNIPIVVNRHLLDGDMLIMNRQPTLHRPSMQAHRVRIIKCVGAKTLRLHYSNCKAYNADFDGDEMNGHFVQSYAAKAELETLASVPHQYLVPKDGTPLAGLIQDHVIAAVKLTMRDRFFEHADYLDLVYHALRPLFAINSQTSSGPPSIITIKPAICWPKCLWTGKQVISTLLINLTPTGLLPLNATLIGRRTKAELWSGVAPGGPTPLSDVDLVICDGYLASGMLDKSHIGSSSTGLIHCVYEAYGPESASCLLNGISLLANRFLKFIAFTMGLKDILLSHRADEERQRRFSNLKDLGLCAFADTFSLKPDELTESNVKRLYRQAQFPSSTDQSSLKRLAQLDMAIKDRLKRAQDAVCDSAIPGGLYVGFPENNLQLMVHVGAKGGMVNAQQMSVALGQIELEGRRVPLMLSGKTLPSFPPYDVRPRAGGMCTARFLTSLPAQELFFHSMAGRDGLVDTAVKTSRSGYLQRSAIKHLEDLSISYDGTVRDSGSNIIQFRYGDDGVDVCQAGFL-QPIGLHFFADNINLMKIRWHSDKINTDNPAYAHLFEKNILPKPIQMMADQVGVKRQGILHKTVSRHKLAKLFNIGKMAHKALKIMETEQQVSGKDKIMNEIIPEPPCAAAAELRDLCLAKIINAQAPPGDPVGLLAAQSVGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMSGSSNISTPCMEVPIWPTKEAQQLSEQIAKKFYKLKLSETLQLPIGQKVNYVSDSCTFEFNLQPKSVYSDQSHVTPVRVLRFLERILFPDLAHRLNEELKDQGKTSLIKSFALSAMARQQQAGQNKNSEEPAEDADIVNQ 1382 HSP 2 Score: 147.517 bits (371), Expect = 1.404e-31 Identity = 81/226 (35.84%), Postives = 130/226 (57.52%), Query Frame = 3
Query: 3870 EQSEYISKTKANVK----AMLPGVFHNYVIESK-PEPDWIKLYLNLNHKR-----FDVKSILLNIFENCVISSVAGVEKALIFNSNKSKR-FATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTN 4514
EQ IS +A + L F Y + K P W +L + + + D++++++ + V+SSV G+EK +I S ++ F EG N +E+ +Y +FDL++ Y+NNI M+ YGIEAA + ++ E+ VF Y I VNSRHL LI+D+LT G AFNR M++H SP+Q+ +FET V+ L ++D + SP+++++ G++V GTN
Sbjct: 1540 EQENAISHDEARKRITFVTSLHARFSGYDFDRKNSSPKWARLTICMFDPKEGRISIDLETLIMRLINRSVVSSVPGIEKTIIDRSKPNQWMFRVEGINMIEMMRYPQVFDLSQLYTNNITVMNETYGIEAARAALQREVSGVFGHYGIHVNSRHLSLISDYLTKFGVYHAFNRRSMSSHPSPIQQMTFETVANVLKHSLQNDSMDNLVSPSASLVAGRIVHYAGTN 1765
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Cavefish
Match: polr2a (RNA polymerase II subunit A [Source:NCBI gene;Acc:103029187]) HSP 1 Score: 362.844 bits (930), Expect = 4.692e-102 Identity = 318/1089 (29.20%), Postives = 505/1089 (46.37%), Query Frame = 3
Query: 90 ICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNC----HQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYG--KFTNTDTS-----IFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAV-MNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDS---GRHRELTGS-QP--GLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQI---GNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEI--DTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNTKPVLSKVLAKKIDELVV-------KLKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGE--PSTQMTLNTF 3260
+ F +P+E++ +S E G+ + GL D G + C+ C + +C GHFG IE +K V++ + K ++ VCF+C +L L+++ + +IKD L SK+ +K + KN + NK V + ++T ++KG C R + P+IR S ELY K N D+ + PE + E + + S+ LIL M + KY + + +L PP+ PAV M G + T + ++V+ LR R + + A + I E + LQ V D+ G R + S +P ++Q++ K+G R N+MGKRV+F+ R+VI+PDP + +D++GVP + +T+PE + P N D L + V G YPGA Y I G+R + P ++ + ++++ RH GD +I NRQPTLH+ SM H ++ + T R++ S YNADFDGDEMN H PQSL +AE + L V TP+ P+ G++QD + A K RD R E ++L L + + K+P+PAI P LWTGKQI S++ IP + + + P SG + + D ++I+ +G + GI K LG S L G ++ +N+ ++ +L+ G S I D + +N + K + + K+ N E +L + F N++ I D KT SS + +L E NN M G+KG + N++++ +G +EGKR P RTLP F + D P SRGF + +++GL P E FF +M GR+GL+DTAVKT+ +GY+QR LIK +E I V +D TVR++++ ++Q RYG DGL +V M + + + EK++ E N + L + L + + + V+ L+R+F ++ +D +LR I + P A + AQ + P T LN
Sbjct: 19 VQFGIISPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERSGRCQTCAGNMTECPGHFGHIELAKPVFHVGFVTKIMKVLRCVCFFCSKL-----LVDA--NNPKIKDIL-----------SKSKGQPRKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPENDEDLT--------KEKGHGGCGRYQ----PRIR-----RSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRI----SDEEDLILGM----DPKYARPEWMIVTVLPVPPLAVRPAVVMQGSARNQDDLTHKLADIVKINNQLR----RNEQSGAAAHV--IAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSIKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLQIDQVGVPRSIAANMTFPEIVTPFNIDRLQELVRRGNSQYPGA-KYIIRDNGDRIDLRFHPKPSDLHLQIGYKVE--------RHMCDGDIVIFNRQPTLHKMSMMGHRVRIL---PWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNL----LMFLSTWDGKVPQPAIHKPRPLWTGKQIFSLI----IPGHINVIRTHSTHPDDED----SGPYKHISPGDTKVIVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVVNNWLLIEGHSIGIGDSIADAKTYLDIQNTIKKAKQDVIEVIEKAHN---NELEPTPGNTLRQ--------TFENQVNRILNDARDKTGSS---------AQKSLSEY---NNFKSMVVAGSKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESIMVKYDATVRNSINQVVQLRYGEDGL-AGEAVEFQN---MATLKPSNKAFEKKFKFEYTNER-ALRRTLQEDVLKDVMTNAHVQSSLEREFEKMR-EDREILRAIFPTGDSKVVLPCNLARMIWNAQKIFRINPRTPTDLNPL 1005 HSP 2 Score: 83.9593 bits (206), Expect = 1.404e-15 Identity = 55/107 (51.40%), Postives = 69/107 (64.49%), Query Frame = 3
Query: 3132 ENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPIL--ACDDAINSATSVLKKFTKLYLKDI 3446
E LL EI A K SI GE VG LAAQS+GEP+TQMTLNTFH AG S NVTLG+PRL+E++ + S + KTPS+ V +L A DA A +L + L+ +
Sbjct: 1064 EWLLGEIEA-KFNQSIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINI-SKRPKTPSLTVFLLGQAARDA-ERAKDILCRLEHTTLRKV 1167 HSP 3 Score: 68.9366 bits (167), Expect = 4.111e-11 Identity = 41/128 (32.03%), Postives = 67/128 (52.34%), Query Frame = 3
Query: 4116 TEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNH-SSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
T+G + + + + D + SN+I+++ + GIEA +E E+ V VN RHL L+ D +T G + A RH + + PL + SFE + V++E ++G D +K + NIM GQL
Sbjct: 1336 TDGVSLMRVLSEKDV-DPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGINRQDTGPLMKCSFEETVDVLMEASSHGECDPMKGVSENIMLGQLA 1462
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Cavefish
Match: polr2a (RNA polymerase II subunit A [Source:NCBI gene;Acc:103029187]) HSP 1 Score: 362.073 bits (928), Expect = 8.762e-102 Identity = 319/1091 (29.24%), Postives = 504/1091 (46.20%), Query Frame = 3
Query: 90 ICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNC----HQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYG--KFTNTDTS-----IFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAV-MNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDS---GRHRELTGS-QP--GLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQI---GNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEI--DTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNTKPVLSKVLAKKIDELVVK-----------LKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGE--PSTQMTLN 3254
+ F +P+E++ +S E G+ + GL D G + C+ C + +C GHFG IE +K V++ + K ++ VCF+C +L L+++ + +IKD L SK+ +K + KN + NK V + ++T ++KG C R + P+IR S ELY K N D+ + PE + E + + S+ LIL M + KY + + +L PP+ PAV M G + T + ++V+ LR R + + A + I E + LQ V D+ G R + S +P ++Q++ K+G R N+MGKRV+F+ R+VI+PDP + +D++GVP + +T+PE + P N D L + V G YPGA Y I G+R + P ++ + ++++ RH GD +I NRQPTLH+ SM H ++ + T R++ S YNADFDGDEMN H PQSL +AE + L V TP+ P+ G++QD + A K RD R E ++L L + + K+P+PAI P LWTGKQI S++ IP + + + P SG + + D ++I+ +G + GI K LG S L G ++ +N+ ++ +L+ G S I D + +N + K + + K+ N E +L + F N++ I D KT SS + +L E NN M G+KG + N++++ +G +EGKR P RTLP F + D P SRGF + +++GL P E FF +M GR+GL+DTAVKT+ +GY+QR LIK +E I V +D TVR++++ ++Q RYG DGL +V M + + + EK++ E N + L + + E V+K L+R+F ++ +D +LR I + P A + AQ + P T LN
Sbjct: 19 VQFGIISPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERSGRCQTCAGNMTECPGHFGHIELAKPVFHVGFVTKIMKVLRCVCFFCSKL-----LVDA--NNPKIKDIL-----------SKSKGQPRKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPENDEDLT--------KEKGHGGCGRYQ----PRIR-----RSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRI----SDEEDLILGM----DPKYARPEWMIVTVLPVPPLAVRPAVVMQGSARNQDDLTHKLADIVKINNQLR----RNEQSGAAAHV--IAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSIKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLQIDQVGVPRSIAANMTFPEIVTPFNIDRLQELVRRGNSQYPGA-KYIIRDNGDRIDLRFHPKPSDLHLQIGYKVE--------RHMCDGDIVIFNRQPTLHKMSMMGHRVRIL---PWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNL----LMFLSTWDGKVPQPAIHKPRPLWTGKQIFSLI----IPGHINVIRTHSTHPDDED----SGPYKHISPGDTKVIVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVVNNWLLIEGHSIGIGDSIADAKTYLDIQNTIKKAKQDVIEVIEKAHN---NELEPTPGNTLRQ--------TFENQVNRILNDARDKTGSS---------AQKSLSEY---NNFKSMVVAGSKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESIMVKYDATVRNSINQVVQLRYGEDGL-AGEAVEFQN---MATLKPSNKAFEKKFKFEYTN-----ERALRRTLQEDVLKDVMTNAHVQSSLEREFEKMR-EDREILRAIFPTGDSKVVLPCNLARMIWNAQKIFRINPRTPTDLN 1003 HSP 2 Score: 83.9593 bits (206), Expect = 1.386e-15 Identity = 55/107 (51.40%), Postives = 69/107 (64.49%), Query Frame = 3
Query: 3132 ENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPIL--ACDDAINSATSVLKKFTKLYLKDI 3446
E LL EI A K SI GE VG LAAQS+GEP+TQMTLNTFH AG S NVTLG+PRL+E++ + S + KTPS+ V +L A DA A +L + L+ +
Sbjct: 1064 EWLLGEIEA-KFNQSIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINI-SKRPKTPSLTVFLLGQAARDA-ERAKDILCRLEHTTLRKV 1167 HSP 3 Score: 68.9366 bits (167), Expect = 4.603e-11 Identity = 41/128 (32.03%), Postives = 67/128 (52.34%), Query Frame = 3
Query: 4116 TEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNH-SSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
T+G + + + + D + SN+I+++ + GIEA +E E+ V VN RHL L+ D +T G + A RH + + PL + SFE + V++E ++G D +K + NIM GQL
Sbjct: 1336 TDGVSLMRVLSEKDV-DPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGINRQDTGPLMKCSFEETVDVLMEASSHGECDPMKGVSENIMLGQLA 1462
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Cavefish
Match: polr3a (polymerase (RNA) III (DNA directed) polypeptide A [Source:ZFIN;Acc:ZDB-GENE-050208-103]) HSP 1 Score: 329.331 bits (843), Expect = 2.778e-92 Identity = 340/1196 (28.43%), Postives = 508/1196 (42.47%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSL-ISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICD-LGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYS-----QEELYGKFTNTDTSIFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDSGRHRELTG---------SQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQ-----------IGNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQR-EFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQK-GLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKD-----GFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNT-----KPVLSKVLAKKIDELVVK----LKR-DFV--------EISPKDENLLREIIAVKSEYSICPN-------------------------------------GEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDA 3389
+ ICF + E++R + ++ S + + G+ D +G+ PC C + DC GH+G ++ ++ CF+ T G+L+ IC +I S +R + ++ L+ QK LK S + + C S +KC + I K K K +P + DF S+ +E+ + ++ P ++ R + NE L L+ P P D+ L+ PP+ P+V D +T + ++ L ++IK+ + A+ Q I E LQ + + +S EL+G G Q++ K G FR N+ GKRV+F+ R+VISPDP + +DE+ VP+ LTYPE +N N + + + V NGPDV+PGA Q GNR M + LK ++ RH + GD ++ NRQP+LH+ S+ AHI+ + +P + +N KA A+ TP++G PL IQD + + L+D+ F R + +V L +++ + LP+PAII P +LWTGKQI S++LK P+ SC P K + + G + L +D ++I + + G DK LG K + L +G A ++ L+RL YL GFS I D G L E I + + GK L + G A +E + R+ C LRE N+ L+M G+KG N++++ +G I G R P R+LP F+++ P ++GF F SGL P E FF +MAGR+GLVDTAVKT+ +GY+QR L+K LED+ +D TVR + +IIQF YG DGLD P ME K+ L KR L R +P LSK +ELV+ +KR DF EI + +I + +Y I N G AVG L AQS+GEP TQMTL TFH AG + MN+TLG+PR++EI+ S I TP + + DDA
Sbjct: 15 ISHICFGMKSAEQMRQQAHIQVVSKNLYSQDTSHTPLSYGVLDHRMGTSEKDRPCETCGKNLADCLGHYGYLD-----------------LELPCFHIGYFKATIGILQMICKTCSRIMLSKEERQQFLDYLRRPGLAYLQKRGLKKKISDKCRKKNVCLYCSAFNGPVKKCGLLKIIHEKY---------KTTKKVVDPIVSDFLQSFDIAIEHNKEVESLLSRAQENLNPLVVLNLFRRI-PNEDVPLLLMNP-----EAGKPADLILTR--LLVPPLCIRPSV---DDLT-----MKLTEII----FLNDVIKKHRMSGAKT--QMIMEDWDFLQLQCALYINS----ELSGIPLNMAPKKWTRGFVQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLRIDEVAVPVHVAKILTYPEKVNKANIELMRKLVKNGPDVHPGANFIQQRHMQMKRFLKYGNREKM---------AQELKL------GDVVERHMIDGDIVLFNRQPSLHKLSIMAHIVSS--NPYIQ------NNPKATKANL-----------------------------VTPRNGEPLIAAIQDFLTGAYLLTLKDTFFDRAKACQIVASILVGKDEKIKVTLPRPAIIKPMRLWTGKQIFSLILK---------PSQSC--PVKANLRTKGKQYCGKGEDLCSNDSFVVIQNSELLCGSLDKGTLGSGSKNNIFYILLRDWGQLEAANAMSRLARLAPVYLSNRGFSIGIGDVTPGQGLLKAKQELLDAGYKKCDEYIEALNTGK-----LQQQPGCTAEETLEALILRELSVIRDHAG------------------SAC---LRELDKSNSPLIMALCGSKGSFINISQMIACVGQQAISGSRVPDGFENRSLPHFEKHSKLPAAKGFVADSFYSGLTPTEFFFHTMAGREGLVDTAVKTAETGYMQRRLVKSLEDLCSQYDLTVRSSTGDIIQFIYGGDGLD---------PAAME--GKDEPLEFKRVLDNIRAVYTCPNEPALSK------NELVLTTDSIMKRTDFACCRDTFLQEIKKFISGVSEKIKKTRDKYGINDNGSTEPKVLYQLDRITPTQLEKFLETCRDKYMRAQMEPGSAVGALCAQSIGEPGTQMTLKTFHFAGVASMNITLGVPRIKEIIN-ASKNISTPIITAHLDTEDDA 1051 HSP 2 Score: 58.9214 bits (141), Expect = 4.465e-8 Identity = 42/161 (26.09%), Postives = 78/161 (48.45%), Query Frame = 3
Query: 4047 VISSVAGVEKALIFNSNKSKR----FATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTN-HSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTN 4514
V+ + V +A+I ++S R EG N + + + ++ SNN ++ + GIEAA + I NEI+ ++ + ++ RH+ L+AD +++ G++ R + S L ASFE + + +G D + + I+ G + NIGT
Sbjct: 1156 VVQGIPEVARAVIHIDDQSGRQKFKLLVEGDNLRAVMATHGV-NGSRTTSNNTYEVEKTLGIEAARTTIINEIQYTMVNHGMSIDRRHVMLLADLMSYKGEILGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMG-IPMNIGTG 1314
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Cavefish
Match: ENSAMXT00000011874.2 (pep primary_assembly:Astyanax_mexicanus-2.0:24:12476100:12482137:1 gene:ENSAMXG00000011553.2 transcript:ENSAMXT00000011874.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 153.68 bits (387), Expect = 1.174e-38 Identity = 87/167 (52.10%), Postives = 116/167 (69.46%), Query Frame = 3
Query: 2514 NLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEK 3014
L FP+NNL LM Q+GAKG SN+ I+ LG IE+EG+R PL G++LP F+ Y+ +P S GF RF++G+KP E FF MAGR+GLVDTAVKTS SGYLQRC+IKHLE + V +D TVRD+ +++QF YG DGLD+ + L +P I +N +++ K
Sbjct: 25 GLHRSFPENNLQLMVQSGAKG-RSNIC-ISCLLGQIELEGRRPPLMPSGKSLPCFQPYEAEPRSGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCVIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFL-QPRQFPFIKENYEVIRK 188 HSP 2 Score: 128.257 bits (321), Expect = 3.529e-30 Identity = 75/171 (43.86%), Postives = 102/171 (59.65%), Query Frame = 3
Query: 3141 LREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQLPFKITVG-DYYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKK-TFKQTLINITDNK 3647
LR ++ +K + ++C GEAVGLLAAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPRLREIL++ S IKTP M +PIL T VL K L + +++ KI+ + +TF F YK L + +++ E F + + +KK + K IN D +
Sbjct: 336 LRNLLQLKWQRALCDPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILLIASSNIKTPMMSIPILR-----KKLTRVLHKVDVL---ETLRMTKKISQNKQIFTVTFHFLPPERYKEDKLLSPQSILSFMEQRFFRLLLEAIKKLSAKLASINAVDTR 498
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Cavefish
Match: ENSAMXT00000011881.2 (pep primary_assembly:Astyanax_mexicanus-2.0:24:12464356:12466989:1 gene:ENSAMXG00000011559.2 transcript:ENSAMXT00000011881.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 95.5153 bits (236), Expect = 6.531e-21 Identity = 69/207 (33.33%), Postives = 97/207 (46.86%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLE------SICDLGQIK-----DSLIKRFNDYENMKSKTLSDKQKNALK---IMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMK 659
L+ + F Y+ EE+R LS K IT+ I ++ V G A NGLYD +LG + KE C C F C GHFG I+ VYNPLLFD L I+ C CH + + + D+G I+ D +K+F + S A I +S+ R H C KS + + K VH+S+++ P+CK K
Sbjct: 12 LEGMSFGMYSTEEIRKLSVKSITNSILLDNV-GTAAPNGLYDLALGPADSKEICTTCMQDFTHCPGHFGHIDLHLPVYNPLLFDKLYLIIRGSCLSCHMFTCPRAAIHLLLNQLKLLDVGAIREVYEIDVALKQFLEGSPKASGAEIQDTLEAFSAPIIKASKGRDGSHVCEIKSSLIRDFWK----VHMSSRKC-----PNCKAGK 208
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Yeast
Match: RPA190 (RNA polymerase I largest subunit A190 [Source:SGD;Acc:S000005868]) HSP 1 Score: 562.377 bits (1448), Expect = 2.138e-173 Identity = 371/1037 (35.78%), Postives = 537/1037 (51.78%), Query Frame = 3
Query: 855 TDVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREIIK-----RKDSTAAEQ---YYQNIREGILELQKRVNVCYDSGRHRELTGSQ---PGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVVENLKFQL----QNNQTVIIN----RHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGL-----HNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETC-KNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDF--------------------MEV-----IAKNIQLMEKRYLKEPRNTK-----PVLSKV------------LAKKIDELVVKLKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDII-QLPFKITVGD----------YYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKKTFKQTLINITDNKDEDNQAVQTDLVNEDELNNPETEANNPE 3731
D FFM+ +++ P RLP+ + G+++ E+ + + V+ +R++ +KD + E + + + +Q VN DS + + T + PG++Q ++KK+GLFR ++MGKRVN+A RSVISPDP I +EIGVP VF KLTYPEP+ N L Q V+NGPD +PGA Q + + + +E + L QL N T +N RH + D ++MNRQPTLH+ SM H ++ + P KTLR+HY+N AYNADFDGDEMN H+PQ+ A+AEA L N Q+ TP GSP+ GLIQDHI AG + +DS FTR + +Y + H + + LP P I P LWTGKQI++ VL N P + N+ KN W E E++ DG + GI DK G S+ G+ L E+YGPEVA K+L+ L RL T Y+ T F+ + D L G R +IL G + + N+ + A + + + NK +D + + Q+V C + +KFP N++ M +GAKG NV++I LG +EG+R P+ V G+TLPSFK Y+ D ++ G+ RF SG+KPQE +F MAGR+GL+DTAVKTS SGYLQRCL K LE + V +D ++RD ++QF YG D +D+ +++ +F ++V +K K++ KEP + PVL+K K++ + K + F +E R ++ +K S+ GEAVG++A+QSVGEPSTQMTLNTFH AG NVTLGIPRLREI+M S IKTP M +PI D + A + K +K+ L ++I ++ T G Y+I F + N+Y + D+ ++EL + N FI + K K+ + +QTD+ N N+ E +N E
Sbjct: 352 ADSFFMDVLVVPPTRFRLPSKL-GEEVHENSQNQLLSKVLTTSLLIRDLNDDLSKLQKDKVSLEDRRVIFSRLMNAFVTIQNDVNAFIDSTKAQGRTSGKVPIPGVKQALEKKEGLFRKHMMGKRVNYAARSVISPDPNIETNEIGVPPVFAVKLTYPEPVTAYNIAELRQAVINGPDKWPGATQIQNEDGSLVSLIGMSVEQRKALANQLLTPSSNVSTHTLNKKVYRHIKNRDVVLMNRQPTLHKASMMGHKVRVL--PNEKTLRLHYANTGAYNADFDGDEMNMHFPQNENARAEALNLANTDSQYLTPTSGSPVRGLIQDHISAGVWLTSKDSFFTREQYQQYIYGCIRPEDGHTTRSKIVTLP-PTIFKPYPLWTGKQIITTVLLNVTPPDMPGINLISKNKIKNEYWGKGSLEN-------EVLFKDGALLCGILDKSQYGASKYGIVHSLHEVYGPEVAAKVLSVLGRLFTNYITATAFTCGMDDLRLTAEGNKWRTDILKTSVDTGREAAAEVTNLD----KDTPADDPELLKRLQEILRDNNKSGILDAVTSSKVNAITSQVVSKCVPDGTMKKFPCNSMQAMALSGAKGSNVNVSQIMCLLGQQALEGRRVPVMVSGKTLPSFKPYETDAMAGGYVKGRFYSGIKPQEYYFHCMAGREGLIDTAVKTSRSGYLQRCLTKQLEGVHVSYDNSIRDADGTLVQFMYGGDAIDITKESHMTQFEFCLDNYYALLKKYNPSALIEHLDVESALKYSKKTLKYRKKHSKEPHYKQSVKYDPVLAKYNPAKYLGSVSENFQDKLESFLDKNSKLFKSSDGVNEKKFRALMQLKYMRSLINPGEAVGIIASQSVGEPSTQMTLNTFHFAGHGAANVTLGIPRLREIVMTASAAIKTPQMTLPIWN-DVSDEQADTFCKSISKVLLSEVIDKVIVTETTGTSNTAGGNAARSYVIHMRFFDNNEYSEEYDVSKEELQNVISNQFIHLLEAAIVKEIKKQKRTTGPDIGVAVPRLQTDVANSSS-NSKRLEEDNDE 1371 HSP 2 Score: 93.9745 bits (232), Expect = 1.457e-19 Identity = 56/186 (30.11%), Postives = 97/186 (52.15%), Query Frame = 3
Query: 3969 W--IKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNSNKSKR-FATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTNA 4517
W KL L + ++ + +I+ I +I + +++ + KR TEG N ++ ++ D++ SN++ + + YG+EAA + I NEI VF Y I V+ RHL LIAD +T G AFNR M +S + S+ET + + + + + SP++ I+ G+L +N+GT +
Sbjct: 1469 WCEFKLELAADTEKLLMVNIVEEICRKSIIRQIPHIDRCVHPEPENGKRVLVTEGVNFQAMWDQEAFIDVDGITSNDVAAVLKTYGVEAARNTIVNEINNVFSRYAISVSFRHLDLIADMMTRQGTYLAFNRQGMETSTSSFMKMSYETTCQFLTKAVLDNEREQLDSPSARIVVGKL-NNVGTGS 1653 HSP 3 Score: 72.0182 bits (175), Expect = 6.330e-13 Identity = 38/100 (38.00%), Postives = 54/100 (54.00%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRL 380
+ S+ F T +E+RNLS K+IT+ ++ LG GLYD +LG+ + C C L C GH G IE YNPL F+ L +++A C +CH
Sbjct: 11 ITSVDFGILTAKEIRNLSAKQITNPTVLDN-LGHPVSGGLYDLALGAFL-RNLCSTCGLDEKFCPGHQGHIELPVPCYNPLFFNQLYIYLRASCLFCHHF 108
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Yeast
Match: RPO21 (RNA polymerase II largest subunit B220; part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime [Source:SGD;Acc:S000002299]) HSP 1 Score: 341.658 bits (875), Expect = 2.554e-96 Identity = 231/688 (33.58%), Postives = 344/688 (50.00%), Query Frame = 3
Query: 1137 LRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGF--------LNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNTKPVLSKVLAKKIDELVVKLK------RDFVEISPKDENLLREI 3152
+R ++ K+G R N+MGKRV+F+ R+VIS DP + LD++GVP LTYPE + P N D L Q V NGP+ +PGA V G+R + ++ QLQ V RH + D ++ NRQP+LH+ SM AH +K I T R++ S YNADFDGDEMN H PQS +AE L V Q +P+ P G++QD + K+ LRD+ ++ L+++Y + +P PAII P LW+GKQILS+ + N I + PK N M +IIDG I G+ +K +G S GL + GP+V K+ N+ +++ +L++ GFST I D + E+ A+K +L+ + + + + L E + + A+ DK+ + + N K L NN+ M G+KG N+ +++ +G +EGKR RTLP F + D P S+GF + ++ GL PQE FF +M GR+GL+DTAVKT+ +GY+QR L+K LEDI V +D T R+++ N+IQF YG DG+D + S ++ I + EKRY + NT L L + E++ LK ++ ++ KD LRE+
Sbjct: 325 IRARLKGKEGRIRGNLMGKRVDFSARTVISGDPNLELDQVGVPKSIAKTLTYPEVVTPYNIDRLTQLVRNGPNEHPGAKYVIRDSGDRIDLRYSKRAGDI------QLQYGWKV--ERHIMDNDPVLFNRQPSLHKMSMMAHRVKVI---PYSTFRLNLSVTSPYNADFDGDEMNLHVPQSEETRAELSQLCAVPLQIVSPQSNKPCMGIVQDTLCGIRKLTLRDTFIELDQVLNMLYW----VPDWDGVIPTPAIIKPKPLWSGKQILSVAIPNGIHLQRFDEGTTLLSPKDNGM-----------------LIIDGQIIFGVVEKKTVGSSNGGLIHVVTREKGPQVCAKLFGNIQKVVNFWLLHNGFSTGIGDTIADGPTMREITETIAEAKKKVLDVTKEAQANLLTAKHGMTLRESFEDNVVRFLNEAR-DKAGRL------AEVNLKDL----------------------NNVKQMVMAGSKGSFINIAQMSACVGQQSVEGKRIAFGFVDRTLPHFSKDDYSPESKGFVENSYLRGLTPQEFFFHAMGGREGLIDTAVKTAETGYIQRRLVKALEDIMVHYDNTTRNSLGNVIQFIYGEDGMDAAHIEKQS----LDTIGGSDAAFEKRYRVDLLNTDHTLDPSLLESGSEILGDLKLQVLLDEEYKQLV-KDRKFLREV 946 HSP 2 Score: 76.6406 bits (187), Expect = 2.590e-14 Identity = 38/64 (59.38%), Postives = 49/64 (76.56%), Query Frame = 3
Query: 3174 SICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDV 3365
S+ GE VG+LAAQS+GEP+TQMTLNTFH AG + VT G+PRL+EIL + + +KTPS+ V
Sbjct: 1056 SVVHPGEMVGVLAAQSIGEPATQMTLNTFHFAGVASKKVTSGVPRLKEILNVAKN-MKTPSLTV 1118 HSP 3 Score: 68.1662 bits (165), Expect = 1.041e-11 Identity = 46/183 (25.14%), Postives = 83/183 (45.36%), Query Frame = 3
Query: 4011 VKSILLNIFENCVISSVAGVEKALIFNSNK-------------SKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMT-NHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTNA 4517
+K I + EN + V +E+ ++ ++ T+G N E+ I D + Y+N+ I + + GIEA + + E+ V S VN RH+ L+ D +T G + + RH +++ L R SFE + ++ E +D + + N++ GQ+ + IGT A
Sbjct: 1260 LKKIENTMLENITLRGVENIERVVMMKYDRKVPSPTGEYVKEPEWVLETDGVNLSEVMTVPGI-DPTRIYTNSFIDIMEVLGIEAGRAALYKEVYNVIASDGSYVNYRHMALLVDVMTTQGGLTSVTRHGFNRSNTGALMRCSFEETVEILFEAGASAELDDCRGVSENVILGQM-APIGTGA 1440 HSP 4 Score: 65.4698 bits (158), Expect = 5.864e-11 Identity = 29/97 (29.90%), Postives = 51/97 (52.58%), Query Frame = 3
Query: 90 ICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRL 380
+ F ++PEEVR +S +I +++ + + GL D LGS++ C+ C +C GHFG I+ +K V++ + K + VC +C +L
Sbjct: 17 VQFGLFSPEEVRAISVAKIRFPETMDETQTRAKIGGLNDPRLGSIDRNLKCQTCQEGMNECPGHFGHIDLAKPVFHVGFIAKIKKVCECVCMHCGKL 113
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Yeast
Match: RPO31 (RNA polymerase III largest subunit C160; part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 [Source:SGD;Acc:S000005642]) HSP 1 Score: 297.745 bits (761), Expect = 1.262e-82 Identity = 204/624 (32.69%), Postives = 315/624 (50.48%), Query Frame = 3
Query: 1134 GLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVVENLKF----QLQNNQTV--IINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLK----NSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKG--LCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKP 2969
G Q++ K G FR N+ GKRV+F+ R+VISPDP + +DE+ VP LTYPE + N+ L + ++NGP+V+PGA N + NL++ +L N + ++ RH GD ++ NRQP+LHR S+ +H K +T R++ C YNADFDGDEMN H PQ+ A+AEA L+ V TPK G P+ QD I + +DS + R L+ + + F +P PAI+ P LWTGKQ+ S+++K + + L+ N PK ++ ++ +D +II I +G+ DK LG +K + LR+ YGP+ A + +++L R+L GFS I D + + + +K K V + + ++ +L + + + T ++ L S ++ + C N L N L+M G+KG NV+++ +G I G R P R+LP F + P S+GF + F SGL P E F +++GR+GLVDTAVKT+ +GY+ R L+K LED++ +D TVR + + I+QF YG DGLD + ++P
Sbjct: 352 GFCQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLSIDEVAVPDRVAKVLTYPEKVTRYNRHKLQELIVNGPNVHPGANYLLKRNE----------DARRNLRYGDRMKLAKNLQIGDVVERHLEDGDVVLFNRQPSLHRLSILSHYAKI---RPWRTFRLNECVCTPYNADFDGDEMNLHVPQTEEARAEAINLMGVKNNLLTPKSGEPIIAATQDFITGSYLISHKDSFYDRATLTQLL--SMMSDGIEHFDIPPPAIMKPYYLWTGKQVFSLLIKPNHNSPVVINLDAKNKVFVPPKSKSL------PNEMSQNDGFVIIRGSQILSGVMDKSVLGDGKKHSVFYTILRD-YGPQEAANAMNRMAKLCARFLGNRGFSIGIND--VTPADDLKQK---------------KEELVEIAYHKCDELITLFNKGELETQPGCNEEQT-LEAKIGGLLSKVREEVGDVCINELDNW---NAPLIMATCGSKGSTLNVSQMVAVVGQQIISGNRVPDGFQDRSLPHFPKNSKTPQSKGFVRNSFFSGLSPPEFLFHAISGREGLVDTAVKTAETGYMSRRLMKSLEDLSCQYDNTVRTSANGIVQFTYGGDGLDPLEMEGNAQP 932 HSP 2 Score: 76.2554 bits (186), Expect = 3.564e-14 Identity = 46/110 (41.82%), Postives = 62/110 (56.36%), Query Frame = 3
Query: 3117 ISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDI 3446
IS K EI K + G A+G + AQS+GEP TQMTL TFH AG + MNVTLG+PR++EI+ S I TP ++ +L D+ +A V + K L D+
Sbjct: 1062 ISEKSVRKFLEIALFKYRKARLEPGTAIGAIGAQSIGEPGTQMTLKTFHFAGVASMNVTLGVPRIKEIIN-ASKVISTPIINA-VLVNDNDERAARVVKGRVEKTLLSDV 1169 HSP 3 Score: 64.6994 bits (156), Expect = 1.103e-10 Identity = 43/161 (26.71%), Postives = 82/161 (50.93%), Query Frame = 3
Query: 4041 NCVISSVAGVEKALIFNSNKSKR-FATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTN-HSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTNA 4517
+ V+ + + +A+I + KR EGY ++ D + ++ +N+++++ + GIEAA I EI ++ + V+ RH++L+ D +T+ G+V R ++ S LQ ASFE + + Y D ++ + I+ GQ +S IGT +
Sbjct: 1269 DVVVKGLPDISRAVINIRDDGKRELLVEGYGLRDVMCTDGVIG-SRTTTNHVLEVFSVLGIEAARYSIIREINYTMSNHGMSVDPRHIQLLGDVMTYKGEVLGITRFGLSKMRDSVLQLASFEKTTDHLFDAAFYMKKDAVEGVSECIILGQTMS-IGTGS 1427
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Nematostella
Match: EDO39496 (DNA-directed RNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7S9W9]) HSP 1 Score: 417.157 bits (1071), Expect = 1.290e-123 Identity = 237/589 (40.24%), Postives = 351/589 (59.59%), Query Frame = 3
Query: 1266 IVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVVENLKFQL----QNNQTVIIN-----RHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW------RISGDEQKLK---LSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETC-KNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDF 2975
+VF KLTYP+P+ N L Q V+NGP V+PGA+ + + + + + + + +L +N+ +N RH +GD +++NRQPTLHRPS+ AH ++ + P KT+R+HY+NCKAYNADFDGDEMN H+PQ+ LA+AEA + + + + PKDG+PL+GLIQDH+++G M +R F + + LVY L+ NQ +L P I+ P Q W+GKQ++S +L N IP G + N+ W R D + LK +S+ E++I DG++ G+ DK H G + GL ELYG ++ ++L+ L+R+ T +L + GF+ +KD + E + R+ ++ K R G + +F +P R + + M+ A + M E+D K + +Y + + C L ++FP NNL LM Q+GAKG N +I+ LG IE+EG+R P+ + GR+LPSF YD P + GF RF++G++PQE FF MAGR+GLVDTAVKTS SGYLQRCLIKHLE + V +D TVRD+ +I+QF YG DGLD++ + LS +
Sbjct: 1 MVFAKKLTYPQPVTTWNVKELRQAVINGPLVHPGAILVESEDGKKIRLSETDVNQRQAIAKRLLTPSGDNKGSHVNCKKVYRHIQNGDVMLLNRQPTLHRPSIMAHKVRVL--PSEKTIRLHYANCKAYNADFDGDEMNAHFPQNELARAEAYTIASTNFNYLVPKDGTPLSGLIQDHMVSGVLMTIRGRMFNKLDYQQLVYGALNFKNQ-PIELLMPCILKPTQFWSGKQVISTILLNLIPLGSKRLNLKGKAKISAKNWNTRKPRRQFLDLEVLKGDDMSESEVVIRDGHLLCGVLDKAHYGNTPYGLVHCCNELYGGVMSGELLSALARVFTSFLQFHGFTLGVKDILVTERADKIRRKLIAKGRVSGKDAATSAFGMPEDCHRSD-LEARMQRAHHNSDG---TDMKELDLCMKKKTDHYQDSINKACLPLGLYQRFPGNNLQLMVQSGAKGSAVNCMQISCLLGQIELEGRRPPIMLSGRSLPSFLPYDTSPRAGGFVDGRFLTGIRPQEYFFHCMAGREGLVDTAVKTSRSGYLQRCLIKHLEGLQVAYDLTVRDSDGSIVQFYYGEDGLDILKTKFLSDKQY 582 HSP 2 Score: 122.479 bits (306), Expect = 7.259e-28 Identity = 78/212 (36.79%), Postives = 117/212 (55.19%), Query Frame = 3
Query: 3141 LREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQLPFKITVGD------------YYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKKTF----KQTLINITDNKDEDNQAVQTDLVNED----ELNNPETE 3716
R ++ +K+ S+ GEAVGLLAAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPR+REILM S IKTP+MD+P+LA I A + +K ++ L +++ I V + Y I F K+ Y+++ +++ E F + ++KT + + + +K NQ + + L+++D EL+ E E
Sbjct: 742 FRSLMYLKTLRSLVDPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRMREILMTASANIKTPTMDMPLLAGKKVIKRAKKLQQKLNRVMLSQVLR---SIDVWESLSPKESGTRMRSYRIKMKFLPKSVYEDEFYCSPAKILHYVETVFFKKLIAVVQKTLSSHPRSHPLRLRRSK---NQHLSSSLLDDDNDNAELSGDEGE 947 HSP 3 Score: 112.849 bits (281), Expect = 5.761e-25 Identity = 53/147 (36.05%), Postives = 86/147 (58.50%), Query Frame = 3
Query: 4038 ENCVISSVAGVEKALIFNSNKSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNI 4478
E V+ G+ + + + TEG N F+Y +I +++K Y+N+I ++R YGIEAA I E+K VF Y I V+ RHL L++D++T+ GK+ AFNR + + +SP Q+ SFET + + G ++ +KSP+S +
Sbjct: 1076 EPAVVHETPGINRCFLVEEKTNSVLKTEGVNLQVAFRYPNIINVDKLYTNDIHAVARTYGIEAAARAITKEVKNVFGGYGIHVDPRHLTLVSDYMTYEGKIKAFNRIGIESSASPFQKMSFETTTHFLRGAVLSGDMESLKSPSSRL 1222
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Nematostella
Match: EDO36291 (DNA-directed RNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7SJ34]) HSP 1 Score: 379.793 bits (974), Expect = 1.699e-109 Identity = 352/1125 (31.29%), Postives = 520/1125 (46.22%), Query Frame = 3
Query: 195 GLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSY-----SQEELYGKFTNTDTSIFPEILREHLRLLWSNESENLQLI----LPMLKYN-NFKYPTDVFFMENMLINPPMNRLPAVMN--GDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDSGRHRELTG----SQP-----GLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQI----GNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQR-EFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDE----QKLKLSDVELIIIDGYICTGIFDKVHLGPSQK-GLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKP-DFMEVIAKNIQLMEKRYLKEPRNTKPVLSKVLAKKIDEL--VVKLKRDF-VEISPKDENLLREIIAVKSEYSICPN-------------------------------------GEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTP 3353
G+ D +G+ + C C DC GH+G I+ V++ F ++ VC C R+ L + Q D+L K N+ S L+ K + + +C C + + +K +I VH K +R KI +P I DF+SS+ + +EL + P LR+L + Q I P+L ++ +P D+ ++ PP+ P+V++ E T + +V L ++I++ S A+ Q I E LQ + + +S E++G QP G Q++ K G FR N+ GKRV+F+ R+VISPDP + +D++ VPI LTYPE + N + Q VLNG D +PGA Q RF + G+ L + QL+ TV RH + GD ++ NRQP+LH+ S+ AH+ A P +T R + C YNADFDGDEMN H PQ+ A+AEA L+ V TP++G PL IQD I + +D R+ L L + + +LP PAI P +LWTGKQ+ S++L+ PN + K R G + + L ++D ++I + + G DK LG K + L +G +VA + ++R+ +L GFS I D A++++L S G + N EFR K + + D + K LS H K LRE N L M G+KG N++++ +G I GKR P R+LP FKR+ P ++GF + F SGL P E FF +M GR+GLVDTAVKT+ +GY+QR L+K LED++ +D TVR + +I+QFRYG DGLD + KP DF V + +IQ ++E R ++ +AK I E +DF E S ENL ++I + ++Y + P+ G AVG + AQS+GEP TQMTL TFH AG + MN+TLG+PR++EI+ S KI TP
Sbjct: 54 GVLDPHMGTCSKDRMCETCGKGLADCVGHYGYIDLELPVFHVGHFRSTIIILQCVCKNCCRV------LLPPAEKKQFLDTLKK-----PNISS--LAKKGMKKKIVEKCKKISHCPYCEDLNGFVRKVGLMKI-VHEKYKTTR----------KI-VDPIISDFQSSFDVALENNKELSPLLPKAQEVLNP------LRVL-----DIFQQIPEQDYPLLMFSPGMSHPHDLIMRR--ILAPPVCIRPSVISELASGSNEDDVTMKLTEIV----FLNDVIRKHRSVGAK--VQMIMEDWDFLQLQCALYINS----EMSGIPLNMQPKKGTRGFVQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLRIDQVAVPIHVAKVLTYPERVTQSNLKLMKQLVLNGADQHPGANFIQQRETNTKRF-LKYGNRKL-----IAKQLKIGDTV--ERHLIDGDVVLFNRQPSLHKLSIMAHL--ATVRPH-RTFRFNECVCTPYNADFDGDEMNLHLPQTEEAKAEALTLMGVKNNLVTPRNGEPLIAAIQDFITGAYLLTYKDVFLDRSHIAQLCASILAGKDTGVDIELPPPAIHKPCRLWTGKQVFSVLLR---------PN---PKVKVRMNLRARGKQYTSGEDLCVNDSFVVIHNSDLMCGALDKAVLGSGSKNSVFYVLLRDFGQQVAADAMWRVARICPFFLSNRGFSIGIGDVTPGHGLLQAKEDLL----STGYSKCEDYIN----EFREGKLQTQPGCSAEDTLEA---------VILKELSVIRDH----AGKACLRELHKSNTPLTMAVCGSKGSFINISQMIACVGQQAISGKRIPDGFEDRSLPHFKRHSKLPAAKGFVKNSFFSGLTPTEFFFHAMGGREGLVDTAVKTAETGYMQRRLVKSLEDLSTQYDITVRTSTGDIVQFRYGGDGLDPTDMEGKDKPLDFSRVFS-HIQC--NHVMREERPLTSSRAREIAKGIIESSEFEMCSKDFKTETSDFVENLAKKIEKIHAQYGVVPHSITSNKIVDYVNRCTETQLKLFLSMCREKYLRARMEPGSAVGAICAQSIGEPGTQMTLKTFHFAGVASMNITLGVPRIKEIIN-ASRKISTP 1082 HSP 2 Score: 66.2402 bits (160), Expect = 1.148e-10 Identity = 53/186 (28.49%), Postives = 85/186 (45.70%), Query Frame = 3
Query: 3978 LYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNSNKSKRFATEGYNTL----ELFKYDSIFDL--NKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTN-HSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTN 4514
L+ + R D+ +L+ ++N V +A+I + R E Y L +L S + N SNN I++ R+ GIEAA I NEI K++ + ++ RH+ L+A+ + G++ RH + S L ASFE + E +G D I + I+ G + NIGT
Sbjct: 1185 LHYVMQQLRSDLPGVLIKGYKN--------VTRAIIHIDEEQSRGGEEHYKLLVEGDDLQAVISTMGVKGNGTTSNNTIEVERVLGIEAARQTIMNEIIYTMKNHGMSIDVRHVMLLAELMAFKGEILGITRHGLAKMKESVLMLASFERTADHLFEAALHGRKDPIDGVSECIIMG-MPMNIGTG 1361
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Nematostella
Match: EDO39976 (DNA-directed RNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7S8F9]) HSP 1 Score: 292.352 bits (747), Expect = 4.343e-80 Identity = 201/612 (32.84%), Postives = 322/612 (52.61%), Query Frame = 3
Query: 1137 LRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQI---GNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGEC-ARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKN----NLREKFPD-NNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVI 2945
++Q++ K+G R N+MGKRV+F+ R+VI+PDP + +D++GVP LT+PE + P N D + + V G + YPGA Y I G R + P ++ +L+F + + RH D +I NRQPTLH+ SM H +K + T R++ S YNADFDGDEMN H PQSL ++AE ++ V TP+ P+ G++QD + A K RD ++ ++L L + K+P+PAI+ P LWTGKQI S++ IP + + + P SG + + D + I +C K LG S L + G ++A +++ ++ +L+ G S I G+C A + ++I+ +T K ++ ++E A D+ + T +T +T N +++++ C++ + ++ + NN M G+KG + N++++ +G +EGKR P RTLP F + D P SRGF + +++GL P E FF +M GR+GL+DTAVKTS +GY+QR L+K +E ++V +DGTVR++ + +IQ RYG DG+D I
Sbjct: 344 VKQRLKSKEGRIRGNLMGKRVDFSARAVITPDPNLSIDQVGVPRTIAQNLTFPEIVTPFNIDSMQELVRRGNNQYPGA-KYIIRDNGERVDLRFHPKPSDL--HLQFGYK------VERHIRDNDVIIFNRQPTLHKMSMMGHRVKIL---PWSTFRLNLSVTTPYNADFDGDEMNLHVPQSLESKAEITEIMMVPRNIVTPQSNKPVMGIVQDSLTAVRKFTKRDVFIEKDAMMNL----LMWIPTWDGKIPQPAILKPRPLWTGKQICSMI----IPGNVNVIRTHSTHPDDED----SGPYKHISPGDTKARI----LC-----KKSLGTSSGSLIHIIAMELGHDIARMFYSDVQCVVNNWLLLEGHSIGI--------GDCIADSDTYDEIQR--ATKKAK-----------KEVIEVIEKAHNDELEP-----TPGNTLRQTF-ENQVNRILNDCRDKTGGSAQKSLSEFNNFKAMVVAGSKGSKINISQVIAVVGQQNVEGKRIPFGFRHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTSETGYIQRRLVKAMEGLSVAYDGTVRNSNNQLIQLRYGEDGMDGI 895 HSP 2 Score: 82.4185 bits (202), Expect = 1.329e-15 Identity = 54/107 (50.47%), Postives = 69/107 (64.49%), Query Frame = 3
Query: 3132 ENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPIL--ACDDAINSATSVLKKFTKLYLKDI 3446
E LL EI + +S GE VG LAAQS+GEP+TQMTLNTFH AG S NVTLG+PRL+EI+ + S K KTPS+ V ++ A DA A +VL + L+ +
Sbjct: 1062 EWLLGEI-ETRFNHSHVKPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKEIINV-SKKPKTPSLTVFLIGQAARDA-EMAKAVLCRLEHTTLRKV 1165 HSP 3 Score: 62.3882 bits (150), Expect = 1.682e-9 Identity = 29/102 (28.43%), Postives = 54/102 (52.94%), Query Frame = 3
Query: 78 NLDSICFSFYTPEEVRNLSTKEITSLISIEPVLG-KGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRL 380
++ + F +P+E++ +S + E + G + +NGL D G+++ C+ C + +C GHFG IE +K V++ + K ++ VCFYC +L
Sbjct: 13 DVKRVQFGILSPDEIKQMSVTTDGGVKYSETMEGGRPKLNGLMDPRQGTIDRFSRCQTCAGNMTECPGHFGHIELAKPVFHVGFMTKIVKTLRCVCFYCSKL 114 HSP 4 Score: 61.6178 bits (148), Expect = 3.060e-9 Identity = 46/182 (25.27%), Postives = 82/182 (45.05%), Query Frame = 3
Query: 3975 KLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNSNKSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHS-SPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTNA 4517
K+Y+NL H+ + ++ E F + + T+G + + + + D + SN+I ++ + GIEA +E E+ V VN RHL L+ D +T G + A RH + L R+SFE + V+++ + DY++ + I+ G+L IGT +
Sbjct: 1301 KVYMNLPHEDKKKRIVVTEQGE---------------FKAIQEWLLETDGVSLMRVLSERDV-DPVRTTSNDICEIFSVLGIEAVRKAVEREMDHVISFDGSYVNYRHLALLCDIMTCRGHLMAITRHGINRQEVGALMRSSFEETVDVLMDAAAHAETDYLRGVSECIIMGKL-PRIGTGS 1465
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Nematostella
Match: EDO25572 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8DW37]) HSP 1 Score: 68.5514 bits (166), Expect = 7.237e-12 Identity = 51/197 (25.89%), Postives = 88/197 (44.67%), Query Frame = 3
Query: 2751 ELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHD--GTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNTKPVLSKVLAKKIDELVVKLKRDF-------VEISPKDENLLREIIAVKSEYSICP--------------NGEAVGLLAAQSVGEPSTQMTLNTFHNAG 3272
E F + R GL DTA+KT+ +GYL R L+ +D+ + + GT+R + ++ N +++E Y + T L V+ DE++++ ++ +E SP + +R ++ +S +C G++VG++AAQS+GEP TQ+TL TFH G
Sbjct: 5 EYFISTHGARKGLADTALKTADAGYLTRRLVDVAQDVVINEEDCGTLRGTTTSALK--------------------------DNEEVVEPLYDRILGRTS--LHDVIDPLTDEIIIEAGKEITEDVAARIEESPIEAVEIRSVLTCESRRGVCAKCYGKNLATGYMAQKGDSVGIIAAQSIGEPGTQLTLRTFHVGG 173
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Nematostella
Match: EDO39497 (DNA-directed RNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7S9X0]) HSP 1 Score: 50.447 bits (119), Expect = 8.002e-8 Identity = 28/72 (38.89%), Postives = 38/72 (52.78%), Query Frame = 3
Query: 102 FYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYN 317
Y+ EEVR LS K IT+ + + +L GLYD LG + + C C + + C GHFG IE VY+
Sbjct: 1 LYSREEVRKLSVKRITNPETFDALLHPNH-GGLYDPVLGPTDRDDLCGTCGQNNFSCPGHFGHIELPLPVYH 71
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Medaka
Match: polr1a (RNA polymerase I subunit A [Source:NCBI gene;Acc:101156830]) HSP 1 Score: 592.038 bits (1525), Expect = 0.000e+0 Identity = 384/1042 (36.85%), Postives = 552/1042 (52.98%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICD-LGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEE--------LYGKFTNTDTS----------IFPEILREHLRLLWSNESENLQLILPMLKYNNFK-------YPTDVFFMENMLINP----PMNRLPAVMNGDQMTESPETTNM------CNVVRAEAALREIIKRKDST-AAEQYYQNIREGI-------------LELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVVENLKFQL-------QNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW-----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYL-VYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKN-NLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEK 3014
L I F Y+ EE+R LS K IT+ ++ V G A N LYD +LG + KE C C F +C GHFG IE VYNPL FD L I+ C CH L + + + + L + I+ E++ ++ L + K + R ++ S+ ++ K T H+ K++ + MK + P R ++ +E G TN +T+ + ++H+ +W E L + P L+ YP D+FF E +++ P P+NRL GDQM + +T NM C ++R AL K + A +Q GI + LQ VN+ +DS + +T PG+RQ ++KK+GLFR ++MGKRV++A RSVI PD I +EIG+P+VF +KLTYP+P+ P N L Q VLNGP+V+PGA + ++ E + QL I+NRH +GD L++NRQPTLHRPS+QAH + + P K LR+HY+NCKAYNADFDGDEMN H+PQS L +AEA LV+ Q+ PKDG PLAGLIQDH+++GT+M +R FTR++ +LV+ GL + R KL PA++ P LWTGKQ++S +L N IP N+ + W R + Q + D +++I G + G+ DK H G S GL ELYG E + K+L L+RL T YL +Y GF+ ++D + R+ I+ R++GS ++ +FN+P A S + A + + + D FK +++ + + + C L FPDNNL LM Q+GAKG N +I+ LG IE+EG+R PL G+ LP F+ YD P + GF RF++G+KPQE FF MAGR+GLVDTAVKTS SGYLQRC+IKHLE + V +D TVRD+ +++QF YG DGLD+ + L +P I N +++ +
Sbjct: 12 LSGISFGMYSAEEIRKLSVKTITNPKFLDAV-GNVAPNSLYDLALGPADLKEVCSTCCQDFNNCPGHFGHIELPLPVYNPLFFDKLYLLIRGSCLSCHMLTCPRAAIHLLLNQLKLVDHGAIREVYQIEHVLNQHLEENPKATGDEIEELLRHYTNETIETSVNISENFKP--TKHVVEKKACLINDFWKIHMKTRKCPYCRSGRTLVRREHNSKLIITAPSGAQTNENTNENVMAGRRVYLTASTAKDHIIKIWEKEGFFLSCLFPGLEETASPADGQGGFYP-DLFFTELLVVPPCRFRPINRL-----GDQMFTNGQTVNMQAVMKDCEIIRKLLALMAGEKNAEGEEAPDQMDPTFLSGITGLTLTDKLYNMWIRLQSHVNIVFDSDMDKLMTEKYPGVRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYIETNEIGIPMVFATKLTYPQPVTPWNVKELRQAVLNGPNVHPGASMVINEDGRKTILSPTNFTQREAVAKQLLTPSLGPHKMSMKIVNRHIKNGDVLLLNRQPTLHRPSIQAHCARIL--PGEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYTLVSTNQQYLVPKDGKPLAGLIQDHMVSGTRMTIRGCFFTRDQYTELVFRGLTDKRGR-VKLLPPAVLKPQHLWTGKQVVSTLLLNVIPEKAVPLNLIGRSKIPSKAWIKLPPRAAPGYQPESMCDSQVVIRQGELLVGVLDKAHYGSSAYGLVHCCYELYGGETSGKLLGCLARLFTAYLQLYRGFTLGVEDILVKPGANKRRRKIIESSRNVGSQALQAAFNLP-PGVDPADARSRWQDAHLNPDQR---DFSTADHKFKEVANQVNNDINKVCMPVGLHTSFPDNNLQLMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMPSGKFLPCFQPYDPSPGAGGFVSGRFLTGIKPQEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTPFL-QPKQFPFIEDNFEVIRR 1036 HSP 2 Score: 131.724 bits (330), Expect = 3.195e-30 Identity = 65/180 (36.11%), Postives = 104/180 (57.78%), Query Frame = 3
Query: 3972 IKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNSN-----KSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
+ L +N FD+ +I+ + +N V+ G+ + L+ + K TEG N E+FKY I DLN+ YSN + M+ YGIE A+ IE EIK VF Y I+V+ RHL L+AD++ G NRH + ++SSPLQ+ +FET+ + + G+ D ++SP++ ++ G++V
Sbjct: 1494 VDLAFPVNKVHFDLTAIVSTVAQNAVVMETRGLTRCLLSETTSKSGEKETVLNTEGINMHEMFKYGEILDLNRLYSNEVHAMANTYGIEVALKVIEKEIKDVFAVYGIEVDPRHLSLVADYMCFEGVYKPLNRHAIRSNSSPLQQMTFETSYKFLKQATMLGSHDQLESPSACLVVGKVV 1673 HSP 3 Score: 123.25 bits (308), Expect = 1.132e-27 Identity = 71/171 (41.52%), Postives = 98/171 (57.31%), Query Frame = 3
Query: 3129 DENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQLPFKITVGD-----------YYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKK 3608
D N LR+++ K + S+C GEAVGLLAAQS+GEPSTQMTLNTFH AG+ EMNVTLGIPRLREILM+ S IKTP M +P+ A+ A + +K ++ L +++Q K+ V + + +TF + Y L +++ E F +KK
Sbjct: 1168 DTNSLRQLLHYKWQRSLCDPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASSNIKTPMMSIPVQHTKKALKKAKMLRRKLARVCLSEVLQ---KVDVVETLQVKHFQKFRAFKVTFHLLPPDRYNEDKLLTPSQILHYMETRFFHLLLDAVKK 1335
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Medaka
Match: polr3a (RNA polymerase III subunit A [Source:NCBI gene;Acc:101157164]) HSP 1 Score: 383.645 bits (984), Expect = 1.861e-110 Identity = 346/1186 (29.17%), Postives = 533/1186 (44.94%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSL-ISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYGKFTNTDTSIFPEILREHLRL---LWSNESENLQLILPMLKYNNFKY-----------------PTDVFFMENMLINPPMNRLPAVMNG--DQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDSGRHRELTG---------SQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQ-IGNRFPMVIGH-NPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQR-EFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQK-GLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNK-----IRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLD---------------VINSV----------SLSKPDFM---EVIAKNIQLMEKR--YLKEPRNTKPVLSKVLAKKIDELVV--------KLKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDA 3389
+ ICF + E++R + ++ S + + +G+ D +G+ PC C + DC GH+G ++ ++ F + ++ +C C + T+ DL +K L+ QK LK S+ + C N S +KC + I K K K +P + +F S+ DT+I EH +L L + ENL P++ N F+ P D+ L+ PP+ P+V++ E T + ++ L ++IK+ + A+ Q I E LQ + + +S EL+G G Q++ K G FR N+ GKRV+F+ R+VISPDP + +DE+ VP+ LTYPE +N N + + + V NGPDV+PGA Q N+ + + N ++ + L+F I+ RH + GD ++ NRQP+LH+ S+ AHI + +T R + C YNADFDGDEMN H PQ+ A+AEA +L+ TP++G PL IQD + + L+D+ F R++ +V L +++ LP PAI+ P LWTGKQI S++L+ P+ C P K + + G + L +D ++I + + G DK LG K + L +G A ++ L+RL YL GFS I D A++++L+ I + GK P G A +E + R++ C LRE N+ L+M G+KG N++++ +G I G R P R+LP F+++ P ++GF F SGL P E FF +MAGR+GLVDTAVKT+ +GY+QR L+K LED+ +D TVR +M +IIQF YG DGLD V++++ +LS+ + + I K M R +L+E + +S+ + K D+ + K+ I+P E K + G AVG L AQS+GEP TQMTL TFH AG + MN+TLG+PR++EI+ S I TP + + DDA
Sbjct: 15 ISHICFGMKSAEQMRQQAHIQVVSKNLYSQDTKHTPLPHGVLDHRMGTSEKDRPCLTCGKNLADCLGHYGYLDLELPCFHVGYFKAVIGILQMICKTCSSIMLTKEEKLQFLDL----------------LKRPNLAYLQKRGLKKKISEKCRKRTVCLNCSAFNGTVKKCGLLKIIHEKY---------KTTKKVVDPFVSEFMQSF------------DTAI------EHNKLIEPLLNRAQENLN---PLVVLNLFRRIPDEDIPLLLMNPEAGKPADLIITR--LLVPPLCIRPSVVSDLKSGTNEDDLTMKLTEII----FLNDVIKKHRMSGAKT--QMIMEDWDFLQLQCALYINS----ELSGIPLNMAPKKWTRGFVQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLRIDEVAVPVHVAKILTYPEMVNKANLELMRKLVRNGPDVHPGANFIQNRRNQIKRFLKYGNREKIAQELRF------GDIVERHMIDGDVVLFNRQPSLHKLSIMAHIARV---KPHRTFRFNECVCTPYNADFDGDEMNLHLPQTEEAKAEALILMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTFFDRSKACQIVASILVGKDEKVRISLPHPAILKPMALWTGKQIFSMILR---------PSKDC--PVKANLRTKGKQYCGKGEDLCYNDSFVVIHNSELMCGSMDKGTLGSGSKNNIFYILLRDWGQLEAANAMSRLARLAPVYLSNRGFSIGIGDVTPGHGLLKAKQDLLDDGYKKCDEYIEALQTGKLQQQP-----GCSAEETLEALILRELSVIRDRAG------------------SAC---LRELDKSNSPLIMALCGSKGSFINISQMIACVGQQAISGSRVPDGFENRSLPHFEKHSKLPAAKGFVADSFYSGLTPTEFFFHTMAGREGLVDTAVKTAETGYMQRRLVKSLEDLCSQYDLTVRSSMGDIIQFIYGGDGLDPAAMEGKDEPLEFKRVLDNIRAVYTCPDEPALSQNKLVLTSDAIMKRADFMCCRDSFLEEIKKFIRSMSERIKKTRDKYGINDNGTSEPKVLYQLDRITPTQLEKFLETCRDKYMRAQMEPGSAVGALCAQSIGEPGTQMTLKTFHFAGVASMNITLGVPRIKEIIN-ASKNISTPIITAQLDVEDDA 1095 HSP 2 Score: 58.9214 bits (141), Expect = 4.985e-8 Identity = 44/161 (27.33%), Postives = 79/161 (49.07%), Query Frame = 3
Query: 4047 VISSVAGVEKALIF---NSNKSK-RFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTN-HSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTN 4514
V+ + V +A+I S K+K + EG N + + + ++ SNN ++ + GIEAA S I NEI+ ++ + ++ RH+ L+AD +++ G++ R + S L ASFE + + +G D + + I+ G + NIGT
Sbjct: 1200 VVQGIPEVARAVIHIDEQSGKNKYKLLVEGDNMRAVMATHGV-NGSRTTSNNTYEVEKTLGIEAARSTIINEIQYTMVNHGMSIDRRHVMLLADLMSYKGEILGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMG-IPMNIGTG 1358
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Medaka
Match: polr3a (RNA polymerase III subunit A [Source:NCBI gene;Acc:101157164]) HSP 1 Score: 383.645 bits (984), Expect = 2.235e-110 Identity = 344/1198 (28.71%), Postives = 533/1198 (44.49%), Query Frame = 3
Query: 81 LDSICFSFYTPEEVRNLSTKEITSL-ISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYGKFTNTDTSIFPEILREHLRL---LWSNESENLQLILPMLKYNNFKY-----------------PTDVFFMENMLINPPMNRLPAVMNG--DQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDSGRHRELTG---------SQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQ-IGNRFPMVIGH-NPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQR-EFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMW----RISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQK-GLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNK-----IRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLD---------------VINSVS-----------------------LSKPDFM----EVIAKNIQLMEKRYLKEPRNTKPVLSKVLAKKIDELVV--------KLKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDA 3389
+ ICF + E++R + ++ S + + +G+ D +G+ PC C + DC GH+G ++ ++ F + ++ +C C + T+ DL +K L+ QK LK S+ + C N S +KC + I K K K +P + +F S+ DT+I EH +L L + ENL P++ N F+ P D+ L+ PP+ P+V++ E T + ++ L ++IK+ + A+ Q I E LQ + + +S EL+G G Q++ K G FR N+ GKRV+F+ R+VISPDP + +DE+ VP+ LTYPE +N N + + + V NGPDV+PGA Q N+ + + N ++ + L+F I+ RH + GD ++ NRQP+LH+ S+ AHI + +T R + C YNADFDGDEMN H PQ+ A+AEA +L+ TP++G PL IQD + + L+D+ F R++ +V L +++ LP PAI+ P LWTGKQI S++L+ P+ C P K + + G + L +D ++I + + G DK LG K + L +G A ++ L+RL YL GFS I D A++++L+ I + GK P G A +E + R++ C LRE N+ L+M G+KG N++++ +G I G R P R+LP F+++ P ++GF F SGL P E FF +MAGR+GLVDTAVKT+ +GY+QR L+K LED+ +D TVR +M +IIQF YG DGLD V++++ + + DFM + + + ++YL+E + +S+ + K D+ + K+ I+P E K + G AVG L AQS+GEP TQMTL TFH AG + MN+TLG+PR++EI+ S I TP + + DDA
Sbjct: 15 ISHICFGMKSAEQMRQQAHIQVVSKNLYSQDTKHTPLPHGVLDHRMGTSEKDRPCLTCGKNLADCLGHYGYLDLELPCFHVGYFKAVIGILQMICKTCSSIMLTKEEKLQFLDL----------------LKRPNLAYLQKRGLKKKISEKCRKRTVCLNCSAFNGTVKKCGLLKIIHEKY---------KTTKKVVDPFVSEFMQSF------------DTAI------EHNKLIEPLLNRAQENLN---PLVVLNLFRRIPDEDIPLLLMNPEAGKPADLIITR--LLVPPLCIRPSVVSDLKSGTNEDDLTMKLTEII----FLNDVIKKHRMSGAKT--QMIMEDWDFLQLQCALYINS----ELSGIPLNMAPKKWTRGFVQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLRIDEVAVPVHVAKILTYPEMVNKANLELMRKLVRNGPDVHPGANFIQNRRNQIKRFLKYGNREKIAQELRF------GDIVERHMIDGDVVLFNRQPSLHKLSIMAHIARV---KPHRTFRFNECVCTPYNADFDGDEMNLHLPQTEEAKAEALILMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTFFDRSKACQIVASILVGKDEKVRISLPHPAILKPMALWTGKQIFSMILR---------PSKDC--PVKANLRTKGKQYCGKGEDLCYNDSFVVIHNSELMCGSMDKGTLGSGSKNNIFYILLRDWGQLEAANAMSRLARLAPVYLSNRGFSIGIGDVTPGHGLLKAKQDLLDDGYKKCDEYIEALQTGKLQQQP-----GCSAEETLEALILRELSVIRDRAG------------------SAC---LRELDKSNSPLIMALCGSKGSFINISQMIACVGQQAISGSRVPDGFENRSLPHFEKHSKLPAAKGFVADSFYSGLTPTEFFFHTMAGREGLVDTAVKTAETGYMQRRLVKSLEDLCSQYDLTVRSSMGDIIQFIYGGDGLDPAAMEGKDEPLEFKRVLDNIRAVYTCPDEPALSQNKLVLTSDAIMKRADFMCCRDSFLEELTETGSEKYLEEIKKFIRSMSERIKKTRDKYGINDNGTSEPKVLYQLDRITPTQLEKFLETCRDKYMRAQMEPGSAVGALCAQSIGEPGTQMTLKTFHFAGVASMNITLGVPRIKEIIN-ASKNISTPIITAQLDVEDDA 1107 HSP 2 Score: 58.9214 bits (141), Expect = 4.952e-8 Identity = 44/161 (27.33%), Postives = 79/161 (49.07%), Query Frame = 3
Query: 4047 VISSVAGVEKALIF---NSNKSK-RFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTN-HSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTN 4514
V+ + V +A+I S K+K + EG N + + + ++ SNN ++ + GIEAA S I NEI+ ++ + ++ RH+ L+AD +++ G++ R + S L ASFE + + +G D + + I+ G + NIGT
Sbjct: 1212 VVQGIPEVARAVIHIDEQSGKNKYKLLVEGDNMRAVMATHGV-NGSRTTSNNTYEVEKTLGIEAARSTIINEIQYTMVNHGMSIDRRHVMLLADLMSYKGEILGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMG-IPMNIGTG 1370
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Medaka
Match: polr2a (RNA polymerase II subunit A [Source:NCBI gene;Acc:101160848]) HSP 1 Score: 360.147 bits (923), Expect = 3.207e-101 Identity = 295/978 (30.16%), Postives = 466/978 (47.65%), Query Frame = 3
Query: 90 ICFSFYTPEEVRNLS--TKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFND--------YENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYG--KFTNTDTS-----IFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAV-MNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDS---GRHRELTGS-QP--GLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQI---GNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEI--DTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGL 2936
+ F +P+E++ +S T+ I + E G+ + GL D G + C+ C + +C GHFG IE +K V++ + K ++ VCF+C +L L++S + +IKD L K YE K K + + + NK V ++ + +IT ++KG C R + P+IR S EL+ K N D+ + PE + E + + S+ LIL M + K+ + + +L PP+ PAV M G + T + ++V+ LR R + + A + I E + LQ V D+ G R + S +P ++Q++ K+G R N+MGKRV+F+ R+VI+PDP + +D++GVP + +T+PE + P N D L + V G YPGA Y I G+R + P ++ + ++++ RH GD +I NRQPTLH+ SM H ++ + T R++ S YNADFDGDEMN H PQSL +AE + L V TP+ P+ G++QD + A K RD R E ++L L + + K+P+PAI+ P LWTGKQI S++ IP + + + P SG + + D ++I+ +G + GI K LG S L G ++ +N+ ++ +L+ G S I D + +N + K + + K+ N E +L + F N++ I D KT SS + +L E NN M G+KG + N++++ +G +EGKR P RTLP F + D P SRGF + +++GL P E FF +M GR+GL+DTAVKT+ +GY+QR LIK +E + V +D TVR++++ ++Q RYG DGL
Sbjct: 19 VQFGIISPDELKRMSVTTEGIKYPETTE--GGRPKLCGLMDPRQGVIERSGRCQTCAGNMTECPGHFGHIELAKPVFHVGFISKIMKVLRCVCFFCSKL-----LVDS--NNPKIKDILTKSKGQPRKRLTHVYELCKGKNICE---------------GGEEMDNKFGVEQQDTEEDIT--------KEKGHGGCGRYQ----PRIR-----RSGLELFAEWKHVNEDSQEKKILLSPERVHEIFKRI----SDEEDLILGM----DPKFARPEWMIVTVLPVPPLAVRPAVVMQGSARNQDDLTHKLADIVKINNQLR----RNEQSGAAAHV--IAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSIKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLQIDQVGVPRSIAANMTFPEIVTPFNIDRLQELVRRGNSQYPGA-KYIIRDNGDRIDLRFHPKPSDLHLQIGYKVE--------RHMCDGDIVIFNRQPTLHKMSMMGHRVRIL---PWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNL----LMFLSTWDGKMPQPAILKPRPLWTGKQIFSLI----IPGHINVIRTHSTHPDDED----SGPYKHISPGDTKVIVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVVNNWLLIEGHSIGIGDSIADAKTYLDIQNTIKKAKQDVIEVIEKAHN---NELEPTPGNTLRQ--------TFENQVNRILNDARDKTGSS---------AQKSLSEY---NNFKSMVVAGSKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGL 894 HSP 2 Score: 82.4185 bits (202), Expect = 4.071e-15 Identity = 50/100 (50.00%), Postives = 64/100 (64.00%), Query Frame = 3
Query: 3153 IAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPIL--ACDDAINSATSVLKKFTKLYLKDI 3446
I K SI GE VG LAAQS+GEP+TQMTLNTFH AG S NVTLG+PRL+E++ + S + KTPS+ V +L A DA A +L + L+ +
Sbjct: 1070 IETKFNQSIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINI-SKRPKTPSLTVFLLGQAARDA-ERAKDILCRLEHTTLRKV 1167 HSP 3 Score: 69.707 bits (169), Expect = 2.506e-11 Identity = 41/128 (32.03%), Postives = 67/128 (52.34%), Query Frame = 3
Query: 4116 TEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNH-SSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
T+G + + + + D + SN+I+++ + GIEA +E E+ V VN RHL L+ D +T G + A RH + + PL + SFE + V++E ++G D +K + NIM GQL
Sbjct: 1336 TDGVSLMRVLSEKDV-DPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGINRQDTGPLMKCSFEETVDVLMEAASHGECDPMKGVSENIMLGQLA 1462
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Medaka
Match: polr2a (RNA polymerase II subunit A [Source:NCBI gene;Acc:101160848]) HSP 1 Score: 360.147 bits (923), Expect = 4.171e-101 Identity = 295/978 (30.16%), Postives = 466/978 (47.65%), Query Frame = 3
Query: 90 ICFSFYTPEEVRNLS--TKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFND--------YENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYG--KFTNTDTS-----IFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAV-MNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDS---GRHRELTGS-QP--GLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQI---GNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEI--DTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGL 2936
+ F +P+E++ +S T+ I + E G+ + GL D G + C+ C + +C GHFG IE +K V++ + K ++ VCF+C +L L++S + +IKD L K YE K K + + + NK V ++ + +IT ++KG C R + P+IR S EL+ K N D+ + PE + E + + S+ LIL M + K+ + + +L PP+ PAV M G + T + ++V+ LR R + + A + I E + LQ V D+ G R + S +P ++Q++ K+G R N+MGKRV+F+ R+VI+PDP + +D++GVP + +T+PE + P N D L + V G YPGA Y I G+R + P ++ + ++++ RH GD +I NRQPTLH+ SM H ++ + T R++ S YNADFDGDEMN H PQSL +AE + L V TP+ P+ G++QD + A K RD R E ++L L + + K+P+PAI+ P LWTGKQI S++ IP + + + P SG + + D ++I+ +G + GI K LG S L G ++ +N+ ++ +L+ G S I D + +N + K + + K+ N E +L + F N++ I D KT SS + +L E NN M G+KG + N++++ +G +EGKR P RTLP F + D P SRGF + +++GL P E FF +M GR+GL+DTAVKT+ +GY+QR LIK +E + V +D TVR++++ ++Q RYG DGL
Sbjct: 19 VQFGIISPDELKRMSVTTEGIKYPETTE--GGRPKLCGLMDPRQGVIERSGRCQTCAGNMTECPGHFGHIELAKPVFHVGFISKIMKVLRCVCFFCSKL-----LVDS--NNPKIKDILTKSKGQPRKRLTHVYELCKGKNICE---------------GGEEMDNKFGVEQQDTEEDIT--------KEKGHGGCGRYQ----PRIR-----RSGLELFAEWKHVNEDSQEKKILLSPERVHEIFKRI----SDEEDLILGM----DPKFARPEWMIVTVLPVPPLAVRPAVVMQGSARNQDDLTHKLADIVKINNQLR----RNEQSGAAAHV--IAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSIKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLQIDQVGVPRSIAANMTFPEIVTPFNIDRLQELVRRGNSQYPGA-KYIIRDNGDRIDLRFHPKPSDLHLQIGYKVE--------RHMCDGDIVIFNRQPTLHKMSMMGHRVRIL---PWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNL----LMFLSTWDGKMPQPAILKPRPLWTGKQIFSLI----IPGHINVIRTHSTHPDDED----SGPYKHISPGDTKVIVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVVNNWLLIEGHSIGIGDSIADAKTYLDIQNTIKKAKQDVIEVIEKAHN---NELEPTPGNTLRQ--------TFENQVNRILNDARDKTGSS---------AQKSLSEY---NNFKSMVVAGSKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGL 894 HSP 2 Score: 82.0333 bits (201), Expect = 4.640e-15 Identity = 47/81 (58.02%), Postives = 57/81 (70.37%), Query Frame = 3
Query: 3153 IAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPIL--ACDDA 3389
I K SI GE VG LAAQS+GEP+TQMTLNTFH AG S NVTLG+PRL+E++ + S + KTPS+ V +L A DA
Sbjct: 1070 IETKFNQSIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINI-SKRPKTPSLTVFLLGQAARDA 1149 HSP 3 Score: 69.707 bits (169), Expect = 2.473e-11 Identity = 41/128 (32.03%), Postives = 67/128 (52.34%), Query Frame = 3
Query: 4116 TEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNH-SSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLV 4496
T+G + + + + D + SN+I+++ + GIEA +E E+ V VN RHL L+ D +T G + A RH + + PL + SFE + V++E ++G D +K + NIM GQL
Sbjct: 1336 TDGVSLMRVLSEKDV-DPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGINRQDTGPLMKCSFEETVDVLMEAASHGECDPMKGVSENIMLGQLA 1462
BLAST of DNA-directed RNA polymerase subunit vs. Planmine SMEST
Match: SMESG000021710.1 (SMESG000021710.1) HSP 1 Score: 2940.98 bits (7623), Expect = 0.000e+0 Identity = 1477/1487 (99.33%), Postives = 1481/1487 (99.60%), Query Frame = 3
Query: 60 MKNYDCNLDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYGKFTNTDTSIFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNTKPVLSKVLAKKIDELVVKLKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQLPFKITVGDYYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKKTFKQTLINITDNKDEDNQAVQTDLVNEDELNNPETEANNPEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNAAFMDEEQSEYISKTKANVKAMLPGVFHNYVIESKPEPDWIKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNSNKSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTNAM 4520
MKNYDCNLDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLND+EPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFD LAKFIKAVCFYCHRLNYTQGLLESICDL QIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYGKFTNTDTSIFPEILREHLRLLWSNESENL LILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKIAIALGAIEIEGKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNI+LMEKRYLKEPRNTKPVLSKVLAKKIDEL+VKLKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQLPFKITVGDYYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKKTFKQTLINITDNKDEDNQAVQ DLVNEDELNNPETEANNPEKD DDMSDLSDIDDLESKKQDDDEEEEDILEDDDEVVDENA FMDEEQSEYISKTKANVKAMLPGVFHNYVIESKPEPDWIKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNSNKSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMS+LYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTNAM
Sbjct: 1 MKNYDCNLDSICFSFYTPEEVRNLSTKEITSLISIEPVLGKGAVNGLYDSSLGSLNDREPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDTLAKFIKAVCFYCHRLNYTQGLLESICDLSQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYGKFTNTDTSIFPEILREHLRLLWSNESENLPLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAVMNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDSGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKIAIALGAIEIEGKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIRLMEKRYLKEPRNTKPVLSKVLAKKIDELLVKLKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINSATSVLKKFTKLYLKDIIQLPFKITVGDYYIITFTFKNKNDYKNKTDLKRKELITLFENAFIQTFAKTLKKTFKQTLINITDNKDEDNQAVQADLVNEDELNNPETEANNPEKDHDDMSDLSDIDDLESKKQDDDEEEEDILEDDDEVVDENAVFMDEEQSEYISKTKANVKAMLPGVFHNYVIESKPEPDWIKLYLNLNHKRFDVKSILLNIFENCVISSVAGVEKALIFNSNKSKRFATEGYNTLELFKYDSIFDLNKFYSNNIIKMSKLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGTNAM 1487
BLAST of DNA-directed RNA polymerase subunit vs. Planmine SMEST
Match: SMESG000078620.1 (SMESG000078620.1) HSP 1 Score: 335.109 bits (858), Expect = 2.883e-93 Identity = 271/934 (29.01%), Postives = 432/934 (46.25%), Query Frame = 3
Query: 204 DSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHISAKESRDKGEPSCKRMKIDENPKIRDFKSSYSQEELYGKFTNTDTSIFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPA-VMNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYD------------SGRHRELTGSQPGLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLV-----YHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEI--DTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLD 2939
D G ++ C+ C+ + +C GHFG I ++ VYN + + K +++VCF C +L L I D I+ E MK S +++ L + C + +++ +S +CE ++ ++ + +P +R +D + + +D ++ LY + +F I E ++ N +KY + +L PPM PA VMN T + +V+ +R+ + ++ I E LQ V D SGR + L+Q++ K G RSN+MGKR ++ R+VI+ DP + LD++GVP LT+PE + P NK FL Q V NG +YPGA V + G+R + + P ++ QL + I+ RH L+ D ++ NRQPTLH+ SM H +K + T R++ S YNADFDGDEMN H PQS A AE L V TP+ P+ ++QD + A KM +RD+ E + L+ ++G+ +PKP I P +LWTGKQ+ S++ IP + + P+ SG + + +D ++I +G + GI K LG S L + + N+ ++ +L++ G + I D N + K +NK + ++ K + E SL++ F K+ I + KT SS + +L + N+ LM +GAKG + N++++ +G +EGKR P RTLP F + D P SRGF + +++GL P E FF +M GR+G++DTAVKT+ +GY+QR L+K +E + V +D TVR+ + +IQ RYG DGLD
Sbjct: 2 DPRQGPMDKYSMCQTCSGNVSECPGHFGHINLARPVYNIGYKEKIIKILRSVCFKCSKL------------LVNIHDPKIR-----EIMKKTEGSYRKRFDL------IHEECKKVKTCNVICDESEECEKGLNTIIRQGCNFVQPKFRRNGLDYSMETKDSDDGKGKQPLYA---HQVVEVFKRISNEDCIIMGFN----------------YKYSRPESMILTVLAVPPMAVRPAIVMNHSGKAHDDLTHKLTEIVKVNNEIRQAEENGVASTI------ILEKENSLQYHVATLMDNESTFLPVAIQKSGRPLK------SLKQRLKGKHGRVRSNLMGKRTDYTARTVITADPNLNLDQVGVPRAIAQNLTFPERVTPFNKKFLQQLVRNG-TMYPGAKFVIRENGDR--IDLKRRP----DSSDIQLSYGE--IVERHMLNDDFVVFNRQPTLHKMSMMGHRVKVL---PFSTFRLNLSVTGPYNADFDGDEMNLHMPQSQEATAEIANLARVNKLVITPQSNRPVMSIVQDSLTACNKMTMRDTFLNAAEMMHLLMFLPSWNGV---------MPKPTIAKPQRLWTGKQLFSLI----IPGKVNCNRTHSTHPESED----SGPYKWISHADTRVLIENGQLIMGILCKKTLGSSSGSLMHIIALEQSADDIGNFFNNIQTVVNNWLLFEGHTIGIVDTLYNAATREEIKFNINKAKDDVFDTITKGHS---NELNSTPGRSLLQ--------TFEEKVNMILNEARDKTGSS---------AQKSLSKY---NSFNLMVVSGAKGSKINISQVIACVGQQNVEGKRIPFGFKQRTLPHFIKTDFGPESRGFVENSYLAGLTPTEFFFHAMGGREGIIDTAVKTAETGYIQRRLMKAMESVMVKYDATVRNQIDQLIQLRYGEDGLD 829 HSP 2 Score: 77.411 bits (189), Expect = 9.616e-14 Identity = 39/64 (60.94%), Postives = 49/64 (76.56%), Query Frame = 3
Query: 3189 GEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILAC 3380
GE VG LAA+S+GEP+TQMTLNTFH AG S NVTLG+PRL+EI+ + S KTPS+ + +L
Sbjct: 1017 GEMVGSLAAESLGEPATQMTLNTFHFAGVSAKNVTLGVPRLKEIINV-SRNPKTPSLTIRLLGA 1079 HSP 3 Score: 66.2402 bits (160), Expect = 2.209e-10 Identity = 38/111 (34.23%), Postives = 63/111 (56.76%), Query Frame = 3
Query: 4182 SNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNH-SSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGT 4511
SN+I ++ +GIEAA + IE E+ V VN RHL L+ + +TH G + + +RH + + L R+SFE + +++E + D +K + +IM GQL + +GT
Sbjct: 1287 SNHITEIFETFGIEAARNAIEAEMTNVISFDGSYVNYRHLALLCEIMTHKGFLMSIDRHGINRQETGALARSSFEETVDILMEAGAHAECDPMKGVSESIMLGQL-ARMGT 1396
BLAST of DNA-directed RNA polymerase subunit vs. Planmine SMEST
Match: SMESG000010459.1 (SMESG000010459.1) HSP 1 Score: 333.954 bits (855), Expect = 6.819e-93 Identity = 233/681 (34.21%), Postives = 351/681 (51.54%), Query Frame = 3
Query: 1137 LRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKPDFMEVIAKNIQLMEKRYLKEPRNTKPVLSKVLAKKIDEL------VVKLKRDFVEISPKDENLLREII 3155
L+Q++ K G RSN+MGKR +++ R+VI+ DP + LD++GVP + LT+PE + P NK +L Q V NG +YPGA V + G+R + + P ++ QLQ +TV RH L+ D ++ NRQPTLH+ SM H +K + T R++ S YNADFDGDEMN H PQSL +AE L V TP+ P+ ++QD + A KM RD R E + L+ + L N R LPKPAI P +LWTGKQI S+++ + + + EP++N G + + D +++I DG + +GI K LG S L + P+ A N+ ++ +L+ G + I D + K +++ + ++ K+ N L G E +K N D KT S C K+ NN LM +GAKG + N++++ +G +EGKR P RTLP F + D P SRGF + +++GL P E FF +M GR+GL+DTAVKT+ +GY+QR LIK +E + + +D TVR+ + +IQFRYG DGLD S P F +A+ EKR+ + N + + K++ E+ + KL ++ ++ KD L+R+I+
Sbjct: 327 LKQRLKGKHGRIRSNLMGKRTDYSARTVITADPNLNLDQVGVPRMIAQNLTFPEKVTPFNKHYLQQLVKNG-TMYPGAAFVIRENGDR--IDLKRRP----DSSDIQLQYGETV--ERHMLNDDFVVFNRQPTLHKMSMMCHRVKVL---PFSTFRLNLSVTSPYNADFDGDEMNLHLPQSLETKAEVSDLARVTKLIITPQSNKPVMSIVQDTLTACNKMTTRDVFLNRAEVMHLLMY-LPTWNGR---LPKPAIYKPLRLWTGKQIFSLIIPGRV--NCIRTHSTHPEPEEN------GPYKWISPGDTKVLIEDGELISGILCKKTLGSSAGSLIHIIALEQHPDEAGGFFNNVQTVVNNWLLIEGHTIGIVDALYDAKTREEIKQTIDRAKEAVFETIDKAHNYELQSTPGNTLRQTFE----TTVNKILN-----DARDKTGS----------CAQKSLSKY--NNFNLMVMSGAKGSKINISQVIACVGQQNVEGKRIPFGFQQRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKAMESVMIKYDSTVRNQIDQLIQFRYGEDGLDGAFVEFQSLPTFKPTMAE----FEKRFRFDISNEDELSRYLTEKRVREITTEADTICKLDTEWDQLC-KDRELIRDIV 957 HSP 2 Score: 79.337 bits (194), Expect = 2.068e-14 Identity = 44/87 (50.57%), Postives = 58/87 (66.67%), Query Frame = 3
Query: 3105 DFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDV 3365
D +SP+ + + I + S+ GE VG LAA+S+GEP+TQMTLNTFH AG S NVTLG+PRL+EI+ + S KTPS+ V
Sbjct: 1044 DEYRLSPEAFDWVLGEIKTRFNQSLVQPGEMVGALAAESLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKEIINV-SRNPKTPSLTV 1129 HSP 3 Score: 67.3958 bits (163), Expect = 9.238e-11 Identity = 39/111 (35.14%), Postives = 63/111 (56.76%), Query Frame = 3
Query: 4182 SNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNH-SSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGT 4511
SN+I ++ +GIEAA + IE E+ V VN RHL L+ + +TH G + + +RH + + L R+SFE + +++E + D +K + +IM GQL + IGT
Sbjct: 1342 SNHITEIFETFGIEAARNAIEREMHHVISFDGSYVNYRHLALLCEIMTHKGFLMSIDRHGINRQETGALARSSFEETVDILMEAGAHAECDPMKGVSESIMLGQL-AKIGT 1451 HSP 4 Score: 53.1434 bits (126), Expect = 2.090e-6 Identity = 28/98 (28.57%), Postives = 47/98 (47.96%), Query Frame = 3
Query: 90 ICFSFYTPEEVRNLSTKEITSLISIEPVL-GKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRL 380
+ F + E++ LS +I E GK +GL D G + C+ C S +C GHFG I ++ V++ + + K +++VCF C +L
Sbjct: 17 VQFGILSASEIKRLSVVGENGIIYAEKTQDGKPKKDGLMDPRQGPFDKYSYCQTCGGSVAECPGHFGHINLARPVFHIGFKEKIIKILRSVCFKCSKL 114
BLAST of DNA-directed RNA polymerase subunit vs. Planmine SMEST
Match: SMESG000003379.1 (SMESG000003379.1) HSP 1 Score: 324.324 bits (830), Expect = 7.808e-90 Identity = 287/985 (29.14%), Postives = 452/985 (45.89%), Query Frame = 3
Query: 78 NLDSICFSFYTPEEVRNLSTKEITSLISIEPVL-GKGAVNGLYDSSLGSLNDKEPCRNCNLSFYDCNGHFGRIEFSKLVYNPLLFDILAKFIKAVCFYCHRLNYTQGLLESICDLGQIKDSLIKRFNDYENMKSKTLSDKQKNALKIMSSQTRKNCHQCHNKSLVFKKSRKCEITVHIS----------AKESRDKG----EPSCKRMKIDENPKIRDFKSSYSQEELYGKFTNTDTSIFPEILREHLRLLWSNESENLQLILPMLKYNNFKYPTDVFFMENMLINPPMNRLPAV-MNGDQMTESPETTNMCNVVRAEAALREIIKRKDSTAAEQYYQNIREGILELQKRVNVCYDSGRHRELTGSQPG------LRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGA--VSYQIGNRFPMVIGHNPLEVVENLKFQLQNNQTVIINRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREFKLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEPKKNTMWRISGDEQKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLRELYGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLN-ESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSS------NYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVGGRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLD 2939
++ S+ F + E+ S + +I E G NGL D+ G ++ C C+ S +C GHFG I SK VY+ + + K +++VCF C R+ L DK + +KI+ +T N + ++ ++S+K + V + E+R +G +P KR +D + I+ G+ + T ++L+ + N +L Y F P + +L PPM PA+ MN T + +V+ + E IKR + A I E I +LQ V +D+ T Q L+Q++ K G R+N+MGKR +F R+VI+ DP + LD++GVPI+ LT+PE + P+N++FL V NG + YPGA V + G+R + N ++ L +TV RH ++ D +I NRQPTLH+ SM H +K + T R++ S YNADFDGDEMN H PQ+ ++AE L V TP+ P+ ++QD + A KM RD + E ++L+ +N +P+PAI+ P +LWTGKQ+ S++ IP G+ N + + N +G + + D ++ + DG + +GI K LG S L + + EV ++ R+ +L+ G S I D N E+ + R +I V K NV F++ D +F+ K + +T N + T K+ N+ M +GAKG + N++++ +G +EGKR P RTLP F + D S+GF + +++GL P E FF +M GR+G++DTAVKT+ +GY+QR L+K +E I V +DGTVR+ + ++ QFRYG DGLD
Sbjct: 13 SVKSVQFGLLSATEILKASVLDPEGIIYPEKAENGIAKRNGLMDARQGPVDRYSYCETCSSSVSECPGHFGHINLSKPVYHIGFKEKIIKVLRSVCFKCSRI----------------------------------LIDKNRPEIKILLKKTEFNLKARFD--MIVEQSKKIKSCVSAEMMETDKEQSLSDENRREGCNFLQPKYKRKGLDYSVTIQ------------GEHNDVTT----KVLQAYQVFDIFQRISNEDCLLMGFDYR-FSRPESMIL--TVLAVPPMAVRPAIIMNHSGKAHDDLTHKLTEIVK----VNEDIKRSEQNGAASVI--ILEKINQLQYHVATLFDNESTFLPTAIQKSGRPLKSLKQRLKGKGGRVRANLMGKRTDFTARTVITADPNLNLDQVGVPILIAKNLTFPEIVTPINRNFLQTLVGNGCE-YPGAKYVIKENGDRIDLKRHPNTKDIC------LSIGETV--ERHMINDDFVIFNRQPTLHKMSMMCHRVKVL---PFSTFRLNLSVTAPYNADFDGDEMNLHMPQTQESRAEVAYLARVNRLVITPQSNKPVMSVVQDSLTACDKMTRRDVLLNKAEMMNLLMFLPCWNNV----MPRPAILKPQRLWTGKQLFSLI----IPKGI---NYTRTHSTHNDDEE-NGPYKWITYCDTKVYVEDGQLLSGILCKKSLGSSSGSLIHVIAVEHNYEVIGNFFNDVQRVTNNWLLIQGHSIGINDCLYNPETRDLIRSSIF----------VAKD-NV---------------FSKIDDCQRFKLKCLPGRSLAETFEDSVNKILNEVRDKTGTIAQKSLSKY--NSFNSMVMSGAKGSKINISQVIACVGQQNVEGKRIPFNFKWRTLPHFVKSDYGSESKGFVENSYLAGLTPSEFFFHAMGGREGIIDTAVKTAETGYIQRRLVKAMESIKVEYDGTVRNQIEHVYQFRYGEDGLD 884 HSP 2 Score: 83.5741 bits (205), Expect = 1.365e-15 Identity = 44/74 (59.46%), Postives = 56/74 (75.68%), Query Frame = 3
Query: 3174 SICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPIL--ACDDA 3389
++ P GE VG LAA+S+GEP+TQMTLNTFH AG S NVTLG+PRLREI+ + S KTPS+++ +L A DA
Sbjct: 1068 ALVPPGEMVGALAAESLGEPATQMTLNTFHFAGVSAKNVTLGVPRLREIINV-SRNPKTPSLNIRLLGKAAKDA 1140 HSP 3 Score: 63.5438 bits (153), Expect = 1.463e-9 Identity = 36/111 (32.43%), Postives = 63/111 (56.76%), Query Frame = 3
Query: 4182 SNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMTNHSSP-LQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGT 4511
SN+I ++ GIEAA + IE E+ V VN RHL L+ D +T G + + +RH + + L R++FE + +++E + +D +K + +++FGQL + +GT
Sbjct: 1345 SNHITEIFTTLGIEAARNAIEYEMNNVLSFDGSYVNYRHLGLLCDVMTQKGYLMSMDRHGINRQETGVLARSTFEETVDILLEAGAHADIDPMKGVSESVLFGQL-AKMGT 1454
BLAST of DNA-directed RNA polymerase subunit vs. Planmine SMEST
Match: SMESG000069361.1 (SMESG000069361.1) HSP 1 Score: 316.235 bits (809), Expect = 4.269e-88 Identity = 261/815 (32.02%), Postives = 397/815 (48.71%), Query Frame = 3
Query: 1134 GLRQKIDKKDGLFRSNIMGKRVNFACRSVISPDPTIGLDEIGVPIVFLSKLTYPEPLNPMNKDFLLQCVLNGPDVYPGAVSYQIGNRFPMVIGHNPLEVVENLKF---------QLQNNQTVI-----------INRHALSGDALIMNRQPTLHRPSMQAHIIKAIHDPKAKTLRMHYSNCKAYNADFDGDEMNGHYPQSLLAQAEAKLLVNVCYQHSTPKDGSPLAGLIQDHIIAGTKMCLRDSTFTRNECLDLVYHGLHNSNQREF-KLPKPAIIFPNQLWTGKQILSIVLKNSIPAGLELPNISCSEP--KKNTMWRISGDE-QKLKLSDVELIIIDGYICTGIFDKVHLGPSQKGLCSDLREL--YGPEVALKILTNLSRLLTRYLVYTGFSTSIKDGFLNESGECARKNILNKIRSIGSTSVGKSFNVPLMEFRGEKATSLMEFAQFDKSDKFRNKMTEIDTNFKTLSSNYIHQLVETCKNNLREKFPDNNLLLMCQTGAKGGESNVTKXXXXXXXXXXXXKRCPLTVG-GRTLPSFKRYDLDPVSRGFCVSRFMSGLKPQELFFESMAGRDGLVDTAVKTSTSGYLQRCLIKHLEDITVCHDGTVRDNMHNIIQFRYGYDGLDVINSVSLSKP-DFME--------------VIAKNIQLMEKRYLKEPRNTKPVLSKVLAKKIDELVVKLKRDFVEISPKDENLLREIIAVKSEYSICPNGEAVGLLAAQSVGEPSTQMTLNTFHNAGQSEMNVTLGIPRLREILMLGSDKIKTPSMDVPILACDDAINS-ATSVLKKFTKLYLKDII 3449
G+ Q++ K G FR NIMGKR NF R+VISPDP++ ++EI +P KLT+ E N L + +LNG YPGAV+Y G +I P + + LK +L N + I + RH S D ++ NRQP+LHR S QA I K KT + C +NADFDGDEMN H+PQ+ A+AEA L++ ++P G PL +QD + A + +D+ + E +L+ + + +P+P I+ P +L++GKQ++S+V+ P G P+ P + N + S + L +D ++I + + G K LG K L R L G + + + L ++ L GFS I D ++ S K+R+I V ++F + + + SL+ SD + +++ + S + + CK L ++ N+ L+M G+KG N++++ +G IEGKR G RTL + P+S+GF + F SGL P E F +M+GR+GL+DTAVKT+ +GY+QR LIK LED+ + D TVRD N++QF+YG DG D S P DF + + IQ + K +L L + L K + + + I+ L + K +I G A+G++ QSVGEP+TQMTL TFH AG + MN+T G+PR++EI M IKTP + V + DD S A ++ K ++LK++I
Sbjct: 311 GMIQRLAGKKGRFRGNIMGKRTNFTSRTVISPDPSLKINEISIPEYCAMKLTFTETATAYNIAHLKKLILNGARKYPGAVAYASG-----IISKEPQQPMSQLKLMMNSEVMWTKLDNEKWRIEAARKLKVGDFVQRHIDSTDIVLFNRQPSLHRLSFQAFNIVV---KKEKTFSFNPCCCNPFNADFDGDEMNVHFPQTFEAKAEAIELISSIKNTTSPNSGEPLTAPLQDILTAAYLLTSKDTFYNEQEFRNLILPIVKCCPPNKIVMIPRPMIMKPVKLYSGKQLMSLVIS---PYGTLSPDSKRVIPDSRINQIVETSSNPVDYLNPNDTLVVIHNSKLIMGQLPKKLLGGGSK-LSIFFRILKDVGDLASCQSMERLCQIALADLKSRGFSLGITD--VSPSA---------KLRTIQKELVEQNFAICEEFIKYYENGSLI-------SDPGCSIEETLESKISSQLSKIRDEGGKECKTTLHKR---NSPLIMAICGSKGSYINISQMVFCVGQQIIEGKRVSNISGTNRTLMYYPENCRSPMSKGFVSNSFYSGLNPIEFLFHAMSGREGLIDTAVKTAETGYMQRRLIKFLEDLAIRFDNTVRDFTGNLVQFKYGTDGFDPSKLESTYCPCDFTKELYHALQDYTYNEPALDNEIQYILKEFLDSHERKFENLDENLVKTLSDYYNSTEGLVFGITRSQMKLFIDNCYQKYFKAIIEPGTAIGVICGQSVGEPATQMTLKTFHFAGVASMNITRGVPRMQEI-MNAVANIKTPIITVYL--TDDVRESFAKTMQLKIQPVFLKNVI 1089 HSP 2 Score: 67.3958 bits (163), Expect = 8.733e-11 Identity = 45/165 (27.27%), Postives = 91/165 (55.15%), Query Frame = 3
Query: 4023 LLNIFENCVISSVAGVEKALIFNSNKSKR-FATEGYNTLELFKYDSIFDLNKFYSNNIIKMSRLYGIEAAMSCIENEIKTVFKSYNIDVNSRHLRLIADFLTHSGKVGAFNRHMMT-NHSSPLQRASFETALTVIIEMLTYGTVDYIKSPTSNIMFGQLVSNIGT 4511
+L + I V V++ +I + +++ EG L+LF+ + + +N +SN+I+++ + GI+AA I NE+ V ++N+ + RH+ L+AD + ++G++ F R ++ + +S L ASFE L ++E + D + + ++ FG+ + IGT
Sbjct: 1161 VLKVIGKVQIMGVKNVKRTVIQRNEQNRHNIYVEGGKLLDLFQIEGVDGINT-HSNHILEIYEVLGIDAAKDMIINELNAVMGAHNLTPDIRHVMLLADIMCYTGQICGFQRSGISKSKNSVLCLASFEKTLDHLLEAAYHNQKDKLHGISDSLTFGK-TTPIGT 1323 The following BLAST results are available for this feature:
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 3
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 4
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of DNA-directed RNA polymerase subunit vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of DNA-directed RNA polymerase subunit vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 3
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of DNA-directed RNA polymerase subunit vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of DNA-directed RNA polymerase subunit vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30001693 ID=SMED30001693|Name=DNA-directed RNA polymerase subunit|organism=Schmidtea mediterranea sexual|type=transcript|length=4522bpback to top protein sequence of SMED30001693-orf-1 >SMED30001693-orf-1 ID=SMED30001693-orf-1|Name=SMED30001693-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1506bp QFGRQKCCLNIQFLILFFNMKNYDCNLDSICFSFYTPEEVRNLSTKEITSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
