SMED30001687

Overview
NameSMED30001687
Smed IDSMED30001687
Length (bp)654
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30001687 (SMED30001687) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30001687 (SMED30001687) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30001687

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30001687 SmedASXL_019376SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30001687 SmedASXL_018878SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30001687 SmedASXL_018878SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30001687 dd_Smed_v4_1857_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30001687 dd_Smed_v4_1857_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30001687 dd_Smed_v4_1857_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30001687 dd_Smed_v4_826_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30001687 dd_Smed_v6_1857_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30001687 OX_Smed_1.0.22960ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30001687 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000093:212330..213052 -1694
Homology
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BLAST of SMED30001687 vs. Planmine SMEST
Match: SMESG000025495.1 (SMESG000025495.1)

HSP 1 Score: 181.8 bits (460), Expect = 1.848e-58
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = -3 
Query:   77 KMNTSRMTAIIFITMLICITGILSATTRFIPPTTKPKTADQPVGDSCEMVCPGPCVNGRCDWTGTRVPRHKRNMKKRQIDIDLGINI 337
            KMNTSRMTAIIFITMLICITGILSATTRFIPPTTKPKTADQPVGDSCEMVCPGPCVNGRCDWTGTRVPRHKRNMKKRQIDIDLGINI
Sbjct:   70 KMNTSRMTAIIFITMLICITGILSATTRFIPPTTKPKTADQPVGDSCEMVCPGPCVNGRCDWTGTRVPRHKRNMKKRQIDIDLGINI 156
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BLAST of SMED30001687 vs. Planmine SMEST
Match: SMESG000043798.1 (SMESG000043798.1)

HSP 1 Score: 151.754 bits (382), Expect = 1.074e-47
Identity = 73/74 (98.65%), Postives = 73/74 (98.65%), Query Frame = 1 
Query:  361 MNRYLIELFMLSIMSVYILAVDENTADICEHYCSNQEIMEFKDNCFVLCINMAKENRKRGHYFKLGKRSRYFKL 582
            MNRYLIELFMLSIMSVYILAVDENTADICEHYCSNQEIMEFKDNCFVLCINM KENRKRGHYFKLGKRSRYFKL
Sbjct:    1 MNRYLIELFMLSIMSVYILAVDENTADICEHYCSNQEIMEFKDNCFVLCINMVKENRKRGHYFKLGKRSRYFKL 74
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BLAST of SMED30001687 vs. Planmine SMEST
Match: SMESG000025581.1 (SMESG000025581.1)

HSP 1 Score: 105.916 bits (263), Expect = 8.534e-30
Identity = 49/75 (65.33%), Postives = 59/75 (78.67%), Query Frame = -3 
Query:  110 MNTSRMTAIIFITMLICITGILSATTRFIPPTTKPKTADQPVGDSCEMVCPGPCVNGRCDWTGTRVPRHKRNMKK 334
            MN  +++ ++ IT+LICI  +LS T RFIPPTTKPK AD+PVGDSCE VCPGPCVNGRC+W G R    KRN+KK
Sbjct:    1 MNRHQISILLAITVLICIADVLSYTERFIPPTTKPKFADKPVGDSCERVCPGPCVNGRCNWNGKRRVLLKRNVKK 75
The following BLAST results are available for this feature:
BLAST of SMED30001687 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001687 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001687 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001687 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001687 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001687 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001687 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001687 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001687 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001687 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001687 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001687 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001687 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001687 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000025495.11.848e-58100.00SMESG000025495.1[more]
SMESG000043798.11.074e-4798.65SMESG000043798.1[more]
SMESG000025581.18.534e-3065.33SMESG000025581.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001687 ID=SMED30001687|Name=SMED30001687|organism=Schmidtea mediterranea sexual|type=transcript|length=654bp
TTTTTAATTAAAACGATGTAATTAAAAATTGTAATAAAATGTATATTATT
TAAATGCACCAATTTAGGCAAAATTAGATATTTATTCCGAGATCTATATC
TATTTGTCGTTTTTTCATATTTCTTTTATGTCGTGGAACCCTTGTACCTG
TCCAATCGCATCGCCCGTTCACACAAGGACCGGGACAGACCATTTCACAG
CTGTCACCAACAGGTTGATCTGCAGTTTTCGGTTTTGTTGTTGGAGGAAT
GAATCTCGTGGTGGCGGACAGAATACCAGTAATACAAATAAGCATCGTAA
TGAAAATAATTGCAGTCATTCGACTTGTATTCATCTTTCAACAATTCAAA
TAATCCAAAAATGAACAGATATCTGATTGAATTGTTTATGCTCTCAATAA
TGTCAGTGTATATTCTGGCTGTTGACGAGAATACCGCCGATATTTGCGAA
CATTATTGTTCCAATCAAGAAATCATGGAGTTCAAGGATAATTGCTTCGT
TCTCTGCATCAACATGGCGAAGGAAAATCGCAAAAGAGGCCATTACTTTA
AGTTAGGAAAAAGATCCAGATATTTCAAACTCTAAAATATTTGTCATTTA
CCAAATTTTTGTTCAAATGAATAATTAAATATTTGCCAAATTTTAAAAAA
ATAA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-TMSignalP-TMcoord: 1..23
score: 0.608
NoneNo IPR availableTMHMMTMhelixcoord: 7..29