ras and EF-hand domain-containing protein homolog

Overview
Nameras and EF-hand domain-containing protein homolog
Smed IDSMED30001660
Length (bp)3111
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of ras and EF-hand domain-containing protein homolog (SMED30001660) t-SNE clustered cells

Violin plots show distribution of expression levels for ras and EF-hand domain-containing protein homolog (SMED30001660) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of ras and EF-hand domain-containing protein homolog (SMED30001660) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for ras and EF-hand domain-containing protein homolog (SMED30001660) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30001660

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30001660h1SMcG0013917 dd_Smed_v4_4284_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30001660h1SMcG0013917 dd_Smed_v4_4284_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30001660h1SMcG0013917 dd_Smed_v4_4284_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30001660h1SMcG0013917 dd_Smed_v4_4284_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Human
Match: RASEF (RAS and EF-hand domain containing [Source:HGNC Symbol;Acc:HGNC:26464])

HSP 1 Score: 207.608 bits (527), Expect = 2.400e-55
Identity = 105/209 (50.24%), Postives = 138/209 (66.03%), Query Frame = 2
Query: 2012 YMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQ---CC 2629
            +  ++ YKIV AGDA+VGKS+F+ RLC + F  +  AT+G+DF+ KT+ VDG+   LQLWDTAGQERFRS+  +YFRKADGVLLLYD T  +SF+++R+WV+ I  AA   +PI+L  NK+D+RD  A EG+K V    GE LA  +  +F ETSAK G NI++ +  LAR + K  D      I +LT        NSK SPQ   CC
Sbjct:  536 FSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTG------TNSKKSPQMKNCC 738          

HSP 2 Score: 99.7525 bits (247), Expect = 1.224e-20
Identity = 123/453 (27.15%), Postives = 195/453 (43.05%), Query Frame = 2
Query:  353 RATTLFHTLDPMQRGFINKEDLFSMNCDLN--EDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFD-------------KTNNSNYSKNRMC--LNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEK---DFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSPPVSY-KAECKLVQCDP---AYHKANTCVYTREKIDSVT-----------QDSLPDEHVLIVGDSGLSTLRDVTELESEVE 1606
            R  ++F   D  + G + +E+  ++  +L     D   VF +LD D+DG I   +F  G  L + +  ++          ++S   P                   T+    S  R       R  DE       EE++   Y  I+   P   + +  +I++ + + +  S E + L     R +      L  LEEE + +I+    K  K E  K E+   D R Q + +  ++ ++  K     EE  K +S  E+V  L   K Q+ DL  EN   +  L   QT    L+SEL  LK    ++    +++   +R+Y +D  +L +Q++IL+  NRKL D+ND LRSALE S S       I+  SP  +  ++  K +   P    Y +++   Y  E  DS+             DSLP+       DSGLSTLRD  E +SEVE
Sbjct:   12 RLRSVFAACDANRSGRLEREEFRALCTELRVRPADAEAVFQRLDADRDGAITFQEFARGF-LGSLRGGRRRDWGPLDPAPAVSEAGPETHDSEEDEGDEDAAAALATSCGPASPGRAWQDFQARLGDEA-KFIPREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALSDLRRQYETEVGDL-QVTIKKLRKLEEQSKRVSQKEDVAAL---KKQIYDLSMENQKVKKDLLEAQTNIAFLQSELDALKSDYADQSLNTERDLEIIRAYTEDRNSLERQIEILQTANRKLHDSNDGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFIGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCF----DSGLSTLRDPNEYDSEVE 454          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Human
Match: CRACR2A (calcium release activated channel regulator 2A [Source:HGNC Symbol;Acc:HGNC:28657])

HSP 1 Score: 185.652 bits (470), Expect = 4.210e-48
Identity = 88/188 (46.81%), Postives = 125/188 (66.49%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNED------LQVMNP 2563
            P+RL+KIVF G+++VGK++F+ R C D F     AT+GID+R KT+ VD   + LQLWDTAGQER+R +T  +FRKADGV+++YD T  +SF+ VR W+ ++ +A G+ +P++L  NK D       E  + V R LGE LA+E+ LIF E SA SG N  +++  LAR L + ED      +QV +P
Sbjct:  542 PDRLFKIVFVGNSAVGKTSFLRRFCEDRFSPGMAATVGIDYRVKTLNVDNSQVALQLWDTAGQERYRCITQQFFRKADGVIVMYDLTDKQSFLSVRRWLSSVEEAVGDRVPVLLLGNKLD------NEKEREVPRGLGEQLATENNLIFYECSAYSGHNTKESLLHLARFLKEQEDTVREDTIQVGHP 723          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Human
Match: RAB6A (RAB6A, member RAS oncogene family [Source:HGNC Symbol;Acc:HGNC:9786])

HSP 1 Score: 150.599 bits (379), Expect = 1.085e-40
Identity = 83/205 (40.49%), Postives = 120/205 (58.54%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQ--VMNPIIDLTKEAITSQKNSKASPQC 2626
            P R +K+VF G+ SVGK++ ITR   DSF +   ATIGIDF +KT+ ++ + I LQLWDTAGQERFRS+  +Y R +   +++YD T + SF     W++ +R   G+++ I+L  NK+DL D      ++ VS E GE  A E  ++F+ETSAK+G N+ Q    +A  L   E  Q      +ID+  E    Q  S+    C
Sbjct:   10 PLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTIRLQLWDTAGQERFRSLIPSYIRDSAAAVVVYDITNVNSFQQTTKWIDDVRTERGSDVIIMLVGNKTDLAD------KRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMESTQDRSREDMIDIKLEKPQEQPVSEGGCSC 208          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Human
Match: RAB6A (RAB6A, member RAS oncogene family [Source:HGNC Symbol;Acc:HGNC:9786])

HSP 1 Score: 148.673 bits (374), Expect = 4.937e-40
Identity = 82/205 (40.00%), Postives = 120/205 (58.54%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQ--VMNPIIDLTKEAITSQKNSKASPQC 2626
            P R +K+VF G+ SVGK++ ITR   DSF +   ATIGIDF +KT+ ++ + + LQLWDTAGQERFRS+  +Y R +   +++YD T + SF     W++ +R   G+++ I+L  NK+DL D      ++ VS E GE  A E  ++F+ETSAK+G N+ Q    +A  L   E  Q      +ID+  E    Q  S+    C
Sbjct:   10 PLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNVNSFQQTTKWIDDVRTERGSDVIIMLVGNKTDLAD------KRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMESTQDRSREDMIDIKLEKPQEQPVSEGGCSC 208          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Human
Match: RAB6B (RAB6B, member RAS oncogene family [Source:HGNC Symbol;Acc:HGNC:14902])

HSP 1 Score: 148.288 bits (373), Expect = 6.224e-40
Identity = 74/178 (41.57%), Postives = 113/178 (63.48%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQ 2551
            P R +K+VF G+ SVGK++ ITR   DSF +   ATIGIDF +KT+ ++ + + LQLWDTAGQERFRS+  +Y R +   +++YD T + SF     W++ +R   G+++ I+L  NK+DL D      ++ ++ E GE  A E  ++F+ETSAK+G N+ Q    +A  L   E++Q
Sbjct:   10 PLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNLNSFQQTSKWIDDVRTERGSDVIIMLVGNKTDLAD------KRQITIEEGEQRAKELSVMFIETSAKTGYNVKQLFRRVASALPGMENVQ 181          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Celegans
Match: rsef-1 (Ras and EF-hand domain-containing protein homolog [Source:UniProtKB/Swiss-Prot;Acc:Q22908])

HSP 1 Score: 174.481 bits (441), Expect = 2.803e-45
Identity = 84/189 (44.44%), Postives = 124/189 (65.61%), Query Frame = 2
Query: 2021 ERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQ------VMNPII 2569
            ER ++IV  GDA+VGKS+F+ R+    F +   +T+G+DF  KT+ VDG+ + LQLWDTAGQERFRS+  +YFR+ADG +L+YD    +SF+ VRDW+ETI+++    IPI+L  NK D+  R +  G  +V++  G ++A+   ++F+ETSA  G NI   M  L R L   ED++      V+NP +
Sbjct:  459 ERTFRIVMCGDAAVGKSSFVMRVIRRQFTNQLPSTLGVDFHVKTVNVDGRNVALQLWDTAGQERFRSLCKSYFRRADGAILVYDVCAEQSFLRVRDWIETIKESTERSIPIILVGNKVDM--RISTPG--SVAKTDGASMAAAMGVLFMETSALDGSNIDNAMLALTRELMAVEDVEIRSTGVVLNPAV 643          

HSP 2 Score: 96.2857 bits (238), Expect = 4.190e-20
Identity = 98/381 (25.72%), Postives = 184/381 (48.29%), Query Frame = 2
Query:  365 LFHTLDPMQRGFINKEDLFSMNCDLNEDDLLDVFSQLDQDKDGCINVDDFKAGLK--LINKQIHKKNLEKRRSRVNSISSWSPP------QEFDKTNNSNYSKNRMCL-----NNRSSDEGMSL-FS--CEEKICDFYDTISKSS-PDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRL--------QQENINLRSYE-------KDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSP 1411
            LF   D   +G++  EDL  +   L+++DL  +F++LDQD  G I   +F  G +  + + + H  N  +RR+   S++   PP      Q FD  ++S  S+  + +      +  SD  +++ FS  C+E++   Y+ +  S  P    +F  ++ S   +  E   E + L   +  E+  Y   +  +E E + Q+EL   K  + E +++ K+     Q+  +E+S ++   +  ++  K +    E +    E  D+L  +  ENN+ +  L+    E  M++SEL  ++ + + K   L        Q E IN  S         ++ +++ KQLQ+L + NRKL +TN+SLR AL++  S   ++R+ +  +P
Sbjct:    9 LFSLCDSESKGYLTMEDLRKVCPQLDDNDLRFIFTELDQDGSGKIEKLEFLRGFQDTVQHGESHGLNGMQRRA---SVAVEDPPMFLRDEQMFDSESDSTSSRPAIRVFDEEHYHSESDTNINIDFSVPCQEEVLVLYEQLQSSGVPALLRKFERVVGSFHKELSEKKHENERLQRIYASEREMYNRRMEEMESEVDQQLELTEMKARQEERDRLTKEKEEMRQRMSDEMSEMRNNIERLQKMEKALERENERLNHQKELSDKLKVVNEENNDLRQNLAENHLELAMIKSELAQVRCEFDQKQDELSARRGILFQLEPINSLSSSDQASHATEESESVRKQLQLLFDANRKLHETNESLRDALDSRAS---VLRQFNLRTP 383          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Celegans
Match: 4R79.2 (pep chromosome:WBcel235:IV:17480396:17482329:-1 gene:WBGene00007067.1 transcript:4R79.2b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:4R79.2)

HSP 1 Score: 155.606 bits (392), Expect = 1.295e-41
Identity = 83/205 (40.49%), Postives = 124/205 (60.49%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVD-GKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCC 2629
            P+R++K+VF GD++VGK+ F+ R C + F     ATIG+DF  KT+++   + I +QLWDTAGQERFRS+T  YFRKADGV+L++D T  +SF++VR+W++++R    +   + L  NK DL   +  +  ++      E LA E ++ F ETSA +G  I   M ++A  L + ED    N + +  K  I S  N K    CC
Sbjct:  115 PDRIFKVVFVGDSAVGKTCFLHRFCHNRFKPLFNATIGVDFTVKTMKIPPNRAIAMQLWDTAGQERFRSITKQYFRKADGVVLMFDVTSEQSFLNVRNWIDSVRAGVDDATVMCLVGNKMDL---FGSDIARSAVYRAAEKLAVEFKIPFFETSAYTGFGIDTCMRQMAENLQRRED----NHLEEALKLDINS--NYKKRSWCC 310          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Celegans
Match: 4R79.2 (pep chromosome:WBcel235:IV:17480396:17483332:-1 gene:WBGene00007067.1 transcript:4R79.2a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:4R79.2)

HSP 1 Score: 155.992 bits (393), Expect = 6.983e-41
Identity = 83/205 (40.49%), Postives = 124/205 (60.49%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVD-GKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCC 2629
            P+R++K+VF GD++VGK+ F+ R C + F     ATIG+DF  KT+++   + I +QLWDTAGQERFRS+T  YFRKADGV+L++D T  +SF++VR+W++++R    +   + L  NK DL   +  +  ++      E LA E ++ F ETSA +G  I   M ++A  L + ED    N + +  K  I S  N K    CC
Sbjct:  199 PDRIFKVVFVGDSAVGKTCFLHRFCHNRFKPLFNATIGVDFTVKTMKIPPNRAIAMQLWDTAGQERFRSITKQYFRKADGVVLMFDVTSEQSFLNVRNWIDSVRAGVDDATVMCLVGNKMDL---FGSDIARSAVYRAAEKLAVEFKIPFFETSAYTGFGIDTCMRQMAENLQRRED----NHLEEALKLDINS--NYKKRSWCC 394          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Celegans
Match: rab-8 (RAB family; Rab8 [Source:UniProtKB/TrEMBL;Acc:G5EFC1])

HSP 1 Score: 146.362 bits (368), Expect = 1.574e-39
Identity = 74/166 (44.58%), Postives = 111/166 (66.87%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELAR 2524
            L+K++  GD+ VGK+  + R   DSF +  ++TIGIDF+ +TIE+DGK I LQ+WDTAGQERFR++T  Y+R A G++L+YD T   SF ++++W+  I + A +++  ++  NK D+      E R+ VSR+ GE LA E+   F+ETSAK+  NI +    LAR
Sbjct:    8 LFKLLLIGDSGVGKTCVLFRFSDDSFNNSFISTIGIDFKIRTIELDGKKIKLQIWDTAGQERFRTITTAYYRGAMGIILVYDITNERSFENIKNWIRNIEEHAASDVERMIIGNKCDI------EERREVSRDRGEQLAIEYGTKFLETSAKANLNIDEAFFTLAR 167          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Celegans
Match: rab-8 (RAB family; Rab8 [Source:UniProtKB/TrEMBL;Acc:G5EFC1])

HSP 1 Score: 146.362 bits (368), Expect = 1.574e-39
Identity = 74/166 (44.58%), Postives = 111/166 (66.87%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELAR 2524
            L+K++  GD+ VGK+  + R   DSF +  ++TIGIDF+ +TIE+DGK I LQ+WDTAGQERFR++T  Y+R A G++L+YD T   SF ++++W+  I + A +++  ++  NK D+      E R+ VSR+ GE LA E+   F+ETSAK+  NI +    LAR
Sbjct:    8 LFKLLLIGDSGVGKTCVLFRFSDDSFNNSFISTIGIDFKIRTIELDGKKIKLQIWDTAGQERFRTITTAYYRGAMGIILVYDITNERSFENIKNWIRNIEEHAASDVERMIIGNKCDI------EERREVSRDRGEQLAIEYGTKFLETSAKANLNIDEAFFTLAR 167          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Fly
Match: Rab6 (gene:FBgn0015797 transcript:FBtr0080361)

HSP 1 Score: 146.362 bits (368), Expect = 1.744e-39
Identity = 74/171 (43.27%), Postives = 108/171 (63.16%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVL 2530
            P R +K+VF G+ SVGK++ ITR   DSF +   ATIGIDF +KT+ ++ + + LQLWDTAGQERFRS+  +Y R +   +++YD T   SF     W++ +R   G+++ I+L  NK+DL D      ++ VS E GE  A E  ++F+ETSAK+G N+ Q    +A  L
Sbjct:    9 PLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNTNSFHQTSKWIDDVRTERGSDVIIMLVGNKTDLSD------KRQVSTEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAAL 173          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Fly
Match: Rab6 (gene:FBgn0015797 transcript:FBtr0346437)

HSP 1 Score: 146.362 bits (368), Expect = 1.744e-39
Identity = 74/171 (43.27%), Postives = 108/171 (63.16%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVL 2530
            P R +K+VF G+ SVGK++ ITR   DSF +   ATIGIDF +KT+ ++ + + LQLWDTAGQERFRS+  +Y R +   +++YD T   SF     W++ +R   G+++ I+L  NK+DL D      ++ VS E GE  A E  ++F+ETSAK+G N+ Q    +A  L
Sbjct:    9 PLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNTNSFHQTSKWIDDVRTERGSDVIIMLVGNKTDLSD------KRQVSTEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAAL 173          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Fly
Match: Rab1 (gene:FBgn0285937 transcript:FBtr0084104)

HSP 1 Score: 144.821 bits (364), Expect = 5.493e-39
Identity = 70/165 (42.42%), Postives = 106/165 (64.24%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELA 2521
            L+K++  GD+ VGKS  + R   D++    ++TIG+DF+ +TIE+DGK I LQ+WDTAGQERFR++T++Y+R A G++++YDCT  ESF +V+ W+E I + A   +  +L  NKSDL        +K V        A++  + F+ETSAKS  N+ Q    +A
Sbjct:   11 LFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDCTDQESFNNVKQWLEEIERYACENVNKLLVGNKSDLTT------KKVVDHTTAAEYAAQLGIPFLETSAKSATNVEQAFMTMA 169          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Fly
Match: Rab8 (gene:FBgn0262518 transcript:FBtr0308833)

HSP 1 Score: 144.05 bits (362), Expect = 9.527e-39
Identity = 75/181 (41.44%), Postives = 118/181 (65.19%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELAR-VLAKNED-LQVMNP 2563
            L+K++  GD+ VGK+  + R   D+F +  ++TIGIDF+ +TIE+D K I LQ+WDTAGQERFR++T  Y+R A G++L+YD T  +SF ++++W+  I + A  ++  +L  NK +L D      ++ VS+E GE LA E+ + F+ETSAK+  N+ +    LA  + AK E  ++  NP
Sbjct:    8 LFKLLLIGDSGVGKTCILFRFSEDAFNTTFISTIGIDFKIRTIELDNKKIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITQEKSFENIKNWIRNIEENASADVEKMLLGNKCELTD------KRQVSKERGEQLAIEYGIKFMETSAKASINVEEAFLTLASDIKAKTEKRMEANNP 182          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Fly
Match: Rab8 (gene:FBgn0262518 transcript:FBtr0074921)

HSP 1 Score: 144.05 bits (362), Expect = 9.527e-39
Identity = 75/181 (41.44%), Postives = 118/181 (65.19%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELAR-VLAKNED-LQVMNP 2563
            L+K++  GD+ VGK+  + R   D+F +  ++TIGIDF+ +TIE+D K I LQ+WDTAGQERFR++T  Y+R A G++L+YD T  +SF ++++W+  I + A  ++  +L  NK +L D      ++ VS+E GE LA E+ + F+ETSAK+  N+ +    LA  + AK E  ++  NP
Sbjct:    8 LFKLLLIGDSGVGKTCILFRFSEDAFNTTFISTIGIDFKIRTIELDNKKIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITQEKSFENIKNWIRNIEENASADVEKMLLGNKCELTD------KRQVSKERGEQLAIEYGIKFMETSAKASINVEEAFLTLASDIKAKTEKRMEANNP 182          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Zebrafish
Match: rasef (RAS and EF-hand domain containing [Source:ZFIN;Acc:ZDB-GENE-081105-157])

HSP 1 Score: 194.126 bits (492), Expect = 3.533e-51
Identity = 96/203 (47.29%), Postives = 133/203 (65.52%), Query Frame = 2
Query: 2021 ERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCC 2629
            E+ Y+IV AGDA+VGKS+F+ RLC + F  +  AT+G+DF+ KT+ VDG    LQLWDTAGQERFRS+  +YFR+ADGVLLLYD T  +SF++VR+WV+ I   + ++IPI+L  NK+DLR    ++G   +    GE LA  +  +F ETSAK G NI++ +  LAR + K+ D     P+         ++K S  SP CC
Sbjct:  505 EKAYRIVLAGDAAVGKSSFLLRLCKNEFKGNTSATLGVDFQMKTLVVDGVPTVLQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCEKSFLNVREWVDIIEDVSQDDIPIMLVGNKTDLRKEALQDGVTCIPTSYGEKLAMTYSALFCETSAKDGSNIIEAVLHLAREVTKHAD-DYKEPVSVAKLSGNHTKKMS--SPNCC 704          

HSP 2 Score: 89.7373 bits (221), Expect = 8.647e-18
Identity = 107/444 (24.10%), Postives = 193/444 (43.47%), Query Frame = 2
Query:  353 RATTLFHTLDPMQRGFINKEDLFSMNCDLN--EDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQ-------LSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSPPVSYKAECKLVQCDPAYHK-ANTCV-------------YTREKIDSVT-------------QDSLPDEHVLIVGDSGLSTLR 1576
            R   LFH  D    G I K +   +  +L+    ++  +FS+LD D+DG IN+++F  G +  +  + +  +    +   S ++W   ++F         K+R+        +        +++   Y  I  + P    Q+  ++ S + + + L+ E + L     R +      L  +EEE + +I    +     E++K E        + D +I  L++K +         L   EN       K++   L+R+NN   +Q       L  + T    L+SEL +LK  + ++    +++   ++ + ++  NL +Q+++L+  NRKL D+NDSLR+ALE+S S S+  +R+S  SP V       +  C   + +  NTCV               R+  DS+              +DSLP        DSG+STLR
Sbjct:    9 RLRELFHACDVDHSGRIEKWEFEKICSELHVQSSEIDSLFSRLDTDEDGTINLEEFMEGFQE-SHLLKEPEMSGEPAGETSSAAW---EDF---------KSRL-------GDQFKFIPRIDQVATLYQNIRLTEPRMVPQYERVLVSFIKEIRILNTEMEHLALAVKRAQDQMSMQLSEMEEEMDHRIHTAERNTRLQESKKAEATLSSMKHQYDSQICELQQKIQ--------TLQMMENQNRSVSLKEETSALKRQNNELMLQNQKLKQELLESHTNVAFLQSELDSLKSDLTDQSINCERDEALMKCFSEERDNLERQIEMLQVANRKLHDSNDSLRTALESSQSKSK-TQRVSGLSPGVYVNTRKNV--CHSYFDRCCNTCVDEEDYDQFRLMAGMRRQSCDSLALALCDPMRRRSCEEDSLP--------DSGMSTLR 413          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Zebrafish
Match: CABZ01062083.1 (calcium release activated channel regulator 2Aa [Source:NCBI gene;Acc:100330395])

HSP 1 Score: 179.489 bits (454), Expect = 2.530e-46
Identity = 81/176 (46.02%), Postives = 120/176 (68.18%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNED 2545
            P+RL+K+V  G++SVGK++ + R C D F S   AT+GID+  KT+ VD   I LQ+WDTAGQER+RS+T  +FRKADGV+++YD T  ++F  VR W+ ++++ AG +IPI+L  NK+DL      + ++ +   LGE LA + QL+F E SA S  N+  +M  +AR+L + ED
Sbjct:  529 PDRLFKVVLVGNSSVGKTSLLRRFCDDCFHSGTCATVGIDYSVKTLSVDNSQIALQMWDTAGQERYRSITKQFFRKADGVVVVYDITNEQTFTAVRQWLASVQEGAGEDIPIMLLGNKTDL------DSQRVIPLGLGEKLAKDFQLMFYECSAFSNHNVTDSMIHMARILKEQED 698          

HSP 2 Score: 68.5514 bits (166), Expect = 3.245e-11
Identity = 84/363 (23.14%), Postives = 170/363 (46.83%), Query Frame = 2
Query:  308 SRASSTESLLDDLSMRATTLFHTLDPMQRGFINKEDLFSMNCDL--NEDDLLDVFSQLDQDKDGCINVDDFKAGLK-------LINKQIHK-KNLEKRRS-RVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKI---EKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLS-----AAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTND 1339
            S+ + +ES    +  +    F   D   +GFI + D+  +N +L  + DDL +VF  LD D +G +  ++F +G         ++    H  + L  R+S  +   S W   +   +  +       M + N  +    ++F   E++   +  + +  P     F + +  +    KE ++EK+ +     R+   +++ ++ L EE E QI+    +    ++E+     +D   QL  K++E+  L  K K  E + + +LS     + ENV++  +R ++  DL RE ++   +LS  Q +  +L+ +   L ++   +I R+ +      S +++  +L+KQL +LRE+N+ L+D  D
Sbjct:   22 SKGTPSESDCSAVIEKTREFFQICDVEGKGFITRRDMQRLNGELPLSADDLENVFDSLDADANGYLTFEEFSSGFSEFMFGPSVVPADPHGGEELVSRKSPEMLYESQWE--ERLSRGEDDEEKHFCMLMENLGAS---NIFEDPEEVRSLWVQLRRDEPHLLSNFEEFLARVTYQIKEANQEKKEMETALKRKSATHDDEIQRLYEEMEQQIKNEKDRILLEDSERFLTRSQDMEHQLLSKEKELEILSNKQKRLERQCRDLLSEQRETSVENVKL--KRYNE--DLSRELDHTSQELSLAQEQLMLLQEQSSRLHEEREMEIYRVTE------SLQRERTSLLKQLDLLREMNKHLRDEKD 369          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Zebrafish
Match: cracr2ab (calcium release activated channel regulator 2Ab [Source:ZFIN;Acc:ZDB-GENE-041210-94])

HSP 1 Score: 169.474 bits (428), Expect = 1.254e-44
Identity = 80/183 (43.72%), Postives = 120/183 (65.57%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPI 2566
            P+RL+K++  G++SVGK+  + R C   F S   AT+GID+  +T+ +   ++ LQLWDTAGQER+RS+T  +FRKADGV+++YD T  +SF  VR W+ +I++A G+ IP++L  NKSD      +E  + V  +  + LA E  L+F E SA +G N+L+ M  LARVL + ED   +N +
Sbjct:  281 PQRLFKVILVGNSSVGKTALLRRFCDGQFHSATSATVGIDYSVRTLNLGDSHVALQLWDTAGQERYRSITKQFFRKADGVVVIYDITMEDSFSSVRPWLSSIQEAVGDPIPVMLLGNKSD------KENEREVQTKEADLLAEEANLMFYECSAYTGANVLEAMIHLARVLREQEDRVWVNTV 457          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Zebrafish
Match: cracr2ab (calcium release activated channel regulator 2Ab [Source:ZFIN;Acc:ZDB-GENE-041210-94])

HSP 1 Score: 172.17 bits (435), Expect = 1.100e-43
Identity = 80/183 (43.72%), Postives = 120/183 (65.57%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPI 2566
            P+RL+K++  G++SVGK+  + R C   F S   AT+GID+  +T+ +   ++ LQLWDTAGQER+RS+T  +FRKADGV+++YD T  +SF  VR W+ +I++A G+ IP++L  NKSD      +E  + V  +  + LA E  L+F E SA +G N+L+ M  LARVL + ED   +N +
Sbjct:  583 PQRLFKVILVGNSSVGKTALLRRFCDGQFHSATSATVGIDYSVRTLNLGDSHVALQLWDTAGQERYRSITKQFFRKADGVVVIYDITMEDSFSSVRPWLSSIQEAVGDPIPVMLLGNKSD------KENEREVQTKEADLLAEEANLMFYECSAYTGANVLEAMIHLARVLREQEDRVWVNTV 759          

HSP 2 Score: 64.3142 bits (155), Expect = 6.339e-10
Identity = 83/350 (23.71%), Postives = 159/350 (45.43%), Query Frame = 2
Query:  365 LFHTLDPMQRGFINKEDLFSMNCDL--NEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDE-----------GMS-LFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETK----SEAEKIE-KDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSY------EKDWQNLMKQLQILREINRKLQDTND 1339
             FH  D   +GFI + D+  ++ +L    ++L +VF  LD DK+G + +  F +G            L  RR  +    + +P Q+  +    + S++   +     DE           G S +F    ++   +  + K  P     F + +  +    KE   E++ + +   R+ + ++  ++ L EE E QI  I +K+      SE+ +I  +D +  L+ K++E+ +L +K +  E + + + S  +      E + + + L+  N      L  T  E  + + +L  L++Q     SRLQ+E   +  Y      +++ Q+LMKQL +LRE+N+ L+D  D
Sbjct:   50 FFHICDSEGKGFITRTDMRRLHKELPLTAEELENVFDSLDVDKNGFLTLGKFSSGFSSF--------LHGRRVSMTEELTTTPSQKAPEAAGQS-SEDEQVMKTIEDDEEKHFSMLMESLGASNVFEDPSEVRSLWAQLRKDEPHLLSNFEEFLSRVTFQIKEAQHERKEMESALRRKAVTHDTEIQRLYEEMEQQI--INEKDRANLKDSESLQIRSQDLQQLLRGKEQELEQLFQKQRRLERQCRDLHSEQQ------ESRLENVKLKMNNEELSRDLEHTCEELVLAQEQLAILQEQ----ASRLQEER-EMEMYRVTEGLQREKQSLMKQLDLLREMNKHLRDERD 377          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Zebrafish
Match: zgc:162879 (zgc:162879 [Source:NCBI gene;Acc:572256])

HSP 1 Score: 167.162 bits (422), Expect = 1.367e-42
Identity = 83/174 (47.70%), Postives = 113/174 (64.94%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDL 2548
            +Y++V AGDA  GKS+F+ RL  + F  D   T+G+DF+ K + VDG+   LQ+WDTAGQERFRS+  +YFRKA GVLLLYD T   SF++VR+WVE IR++   +IP+ +  NK DLR    E     VS   GE LA  +  +F E SAK G N+++ +  LAR + K+  L
Sbjct:  467 VYRLVLAGDAGSGKSSFLLRLSLNEFRGDIQTTLGVDFQIKKMLVDGEKTNLQIWDTAGQERFRSIARSYFRKAHGVLLLYDVTSESSFLNVREWVEQIRESTDEDIPMCIIGNKVDLRAARPEGS--CVSSIHGEKLAMNYNALFCEASAKEGTNVIEAVLHLAREVKKHVKL 638          

HSP 2 Score: 77.7962 bits (190), Expect = 3.802e-14
Identity = 80/345 (23.19%), Postives = 163/345 (47.25%), Query Frame = 2
Query:  365 LFHTLDPMQRGFINKEDLFSMNCDLN--EDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNL-----EKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRA-EQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKD------QLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSAL 1357
            LF   D  Q G +  ED  ++  +LN   DD+  +F++ D D DG IN +DF +  + +++ ++  +L      +RR+       W    EF+              N    D    L    + + + Y+ I  +S +   +QF D+I +++ + +E   E + L     R +    + L  +EE+ + Q+    ++  + E +K+++   M   K + E++ L+   +   ++++      E  ++ + R+D      Q+ DL  EN   +  L   Q   ++L+ EL  LK    ++  + ++E+ +L+    ++Q+    +++L+E+N+ L D+ND LRSAL
Sbjct:    9 LFAACDGNQSGRVEYEDFTTVCRELNVPADDIRTLFNKFDLDGDGYINFNDFSSSFQEVSEALNLASLGNCLHSQRRA-------WD---EFE--------------NTLDGDVAFYLGRQWDALSELYEGIHSTSDELLLQQFEDLIRALVTEIREHRMESEQLETSLRRTEEVSSSQLAEMEEDLQQQLIHTERRVREEEQKKLDESIAMLQIKHENELADLQTTIERLTKQYQ------EESKLNTPREDSVKLRAQIKDLMEENEELRASLMKAQMNVSILQVELDKLKNAFTDQKRQHERESDDLKKMVMEFQSYSSHIEMLQEMNKSLYDSNDGLRSAL 323          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Xenopus
Match: irf6 (interferon regulatory factor 6 [Source:Xenbase;Acc:XB-GENE-876410])

HSP 1 Score: 194.512 bits (493), Expect = 1.033e-52
Identity = 94/178 (52.81%), Postives = 125/178 (70.22%), Query Frame = 2
Query: 2021 ERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAK---NED 2545
            E+ YKIV AGDA+VGKS+F+ RLC + F  +  AT+G+DF+ KT+ VDG+   LQLWDTAGQERFRS+  +YFR+ADGVLLLYD T  +SF++VR+W++ I  A    IPI++  NK+DLR   AE+G   VS   GE L+  +  +F ETSAK G NI++ +  LAR + K   NED
Sbjct:  305 EKAYKIVLAGDAAVGKSSFLMRLCKNEFRGNTSATLGVDFQMKTLVVDGEPTILQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCEKSFLNVREWIDMIEDATSEAIPIMMVGNKADLRQLMAEQGHICVSTNYGEKLSRTYGALFCETSAKEGSNIVEAVLHLAREVRKRCDNED 482          

HSP 2 Score: 77.411 bits (189), Expect = 5.575e-14
Identity = 71/197 (36.04%), Postives = 96/197 (48.73%), Query Frame = 2
Query: 1067 ENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISC--------TSPPVSYKAE--CKLVQCDPAYHKANTCVYTREKIDSVTQDSLPDEHVLIVGDSGLSTLRDVTELESEVE--PVNVF 1621
            E+V VL  R   L DL  EN   +  L   QT  + L+SEL  LK    ++    +++   +R +  + +NL +Q++IL+  NRKL D+ND LRSALE   S  +  R + C         SP  SY  E    L  CDP   + N  V           DSLP+  +    DSGLSTLRD  E +SE E  P  +F
Sbjct:   54 EDVAVLRRR---LHDLTMENQKLRKDLLEAQTSISFLQSELDALKSDYADQSLSSERDMDIIRGFTDERENLARQIEILQTANRKLHDSNDGLRSALEN--SLMKYNRSLVCLTCSSYLLMSPRSSYLDEDYDSLAVCDP-MQRMNCEV-----------DSLPESCI----DSGLSTLRDSNEYDSEAEYRPPRIF 229          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Xenopus
Match: irf6 (interferon regulatory factor 6 [Source:Xenbase;Acc:XB-GENE-876410])

HSP 1 Score: 194.512 bits (493), Expect = 3.256e-51
Identity = 94/178 (52.81%), Postives = 125/178 (70.22%), Query Frame = 2
Query: 2021 ERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAK---NED 2545
            E+ YKIV AGDA+VGKS+F+ RLC + F  +  AT+G+DF+ KT+ VDG+   LQLWDTAGQERFRS+  +YFR+ADGVLLLYD T  +SF++VR+W++ I  A    IPI++  NK+DLR   AE+G   VS   GE L+  +  +F ETSAK G NI++ +  LAR + K   NED
Sbjct:  496 EKAYKIVLAGDAAVGKSSFLMRLCKNEFRGNTSATLGVDFQMKTLVVDGEPTILQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCEKSFLNVREWIDMIEDATSEAIPIMMVGNKADLRQLMAEQGHICVSTNYGEKLSRTYGALFCETSAKEGSNIVEAVLHLAREVRKRCDNED 673          

HSP 2 Score: 101.293 bits (251), Expect = 3.457e-21
Identity = 128/444 (28.83%), Postives = 203/444 (45.72%), Query Frame = 2
Query:  338 DDLSMRATTLFHTLDPMQRGFINKEDLFSMNCDL--NEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXV--LSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISC--------TSPPVSYKAE--CKLVQCDPAYHKANTCVYTREKIDSVTQDSLPDEHVLIVGDSGLSTLRDVTELESEVE--PVNVF 1621
            D+LS R   LFHT D    G + K    ++  +L  +  +  D+F++LD DKD CI  +DF  G +   +Q                 S S P + +K   ++ S        R +DE ++     E+    Y  I+   P   +Q+  +I + + + +  S E + L     R +      L  LEEE + +I+ + K+  K E  K E+      ++ + E++ L+   K+ ++  ++   ++  E+V VL  R   L DL  EN   +  L   QT  + L+SEL  LK    ++    +++   +R +  + +NL +Q++IL+  NRKL D+ND LRSALE   S  +  R + C         SP  SY  E    L  CDP   + N  V           DSLP+  +    DSGLSTLRD  E +SE E  P  +F
Sbjct:   13 DELS-RLRALFHTFDSKSSGRLEKGQFSALCAELKVSPSEAEDIFARLDSDKDSCITFEDFAMGFRGDVEQGEPPK------------SPSTPDKEEKPEETS-SPAWEDFQRRLADE-VNYIPRREQASILYQNINIVEPLLIQQYEHVIRNFVREIRLQSTEMENLAIAVKRAQDKAAIQLSELEEEMDQRIQAVEKRVKKEEKRKSEEALNDLKRQHESEVAELQVTIKKLKKLEEQSKNINLKEDVAVLRRR---LHDLTMENQKLRKDLLEAQTSISFLQSELDALKSDYADQSLSSERDMDIIRGFTDERENLARQIEILQTANRKLHDSNDGLRSALEN--SLMKYNRSLVCLTCSSYLLMSPRSSYLDEDYDSLAVCDP-MQRMNCEV-----------DSLPESCI----DSGLSTLRDSNEYDSEAEYRPPRIF 420          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Xenopus
Match: irf6 (interferon regulatory factor 6 [Source:Xenbase;Acc:XB-GENE-876410])

HSP 1 Score: 194.512 bits (493), Expect = 3.729e-51
Identity = 94/178 (52.81%), Postives = 125/178 (70.22%), Query Frame = 2
Query: 2021 ERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAK---NED 2545
            E+ YKIV AGDA+VGKS+F+ RLC + F  +  AT+G+DF+ KT+ VDG+   LQLWDTAGQERFRS+  +YFR+ADGVLLLYD T  +SF++VR+W++ I  A    IPI++  NK+DLR   AE+G   VS   GE L+  +  +F ETSAK G NI++ +  LAR + K   NED
Sbjct:  520 EKAYKIVLAGDAAVGKSSFLMRLCKNEFRGNTSATLGVDFQMKTLVVDGEPTILQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCEKSFLNVREWIDMIEDATSEAIPIMMVGNKADLRQLMAEQGHICVSTNYGEKLSRTYGALFCETSAKEGSNIVEAVLHLAREVRKRCDNED 697          

HSP 2 Score: 114.005 bits (284), Expect = 3.821e-25
Identity = 128/446 (28.70%), Postives = 209/446 (46.86%), Query Frame = 2
Query:  338 DDLSMRATTLFHTLDPMQRGFINKEDLFSMNCDL--NEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXV--LSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSPPVSY-KAECKLVQCDPAYHKANTCVYTREKIDSVT-----------QDSLPDEHVLIVGDSGLSTLRDVTELESEVE--PVNVF 1621
            D+LS R   LFHT D    G + K    ++  +L  +  +  D+F++LD DKD CI  +DF  G +   + +H    +K   +     S S P + +K   ++ S        R +DE ++     E+    Y  I+   P   +Q+  +I + + + +  S E + L     R +      L  LEEE + +I+ + K+  K E  K E+      ++ + E++ L+   K+ ++  ++   ++  E+V VL  R   L DL  EN   +  L   QT  + L+SEL  LK    ++    +++   +R +  + +NL +Q++IL+  NRKL D+ND LRSALE S        R   TSP  +  +   KL +C   Y ++    Y  E  DS+             DSLP+  +    DSGLSTLRD  E +SE E  P  +F
Sbjct:    5 DELS-RLRALFHTFDSKSSGRLEKGQFSALCAELKVSPSEAEDIFARLDSDKDSCITFEDFAMGFRGA-RGLHMPEGKKDVEQGEPPKSPSTPDKEEKPEETS-SPAWEDFQRRLADE-VNYIPRREQASILYQNINIVEPLLIQQYEHVIRNFVREIRLQSTEMENLAIAVKRAQDKAAIQLSELEEEMDQRIQAVEKRVKKEEKRKSEEALNDLKRQHESEVAELQVTIKKLKKLEEQSKNINLKEDVAVLRRR---LHDLTMENQKLRKDLLEAQTSISFLQSELDALKSDYADQSLSSERDMDIIRGFTDERENLARQIEILQTANRKLHDSNDGLRSALENSLMKYNRSLRTINTSPGSTISRNSPKLTRCTSPYDRSPRSSYLDEDYDSLAVCDPMQRMNCEVDSLPESCI----DSGLSTLRDSNEYDSEAEYRPPRIF 439          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Xenopus
Match: irf6 (interferon regulatory factor 6 [Source:Xenbase;Acc:XB-GENE-876410])

HSP 1 Score: 193.356 bits (490), Expect = 4.383e-51
Identity = 90/168 (53.57%), Postives = 120/168 (71.43%), Query Frame = 2
Query: 2021 ERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELAR 2524
            E+ YKIV AGDA+VGKS+F+ RLC + F  +  AT+G+DF+ KT+ VDG+   LQLWDTAGQERFRS+  +YFR+ADGVLLLYD T  +SF++VR+W++ I  A    IPI++  NK+DLR   AE+G   VS   GE L+  +  +F ETSAK G NI++ +  LAR
Sbjct:  495 EKAYKIVLAGDAAVGKSSFLMRLCKNEFRGNTSATLGVDFQMKTLVVDGEPTILQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCEKSFLNVREWIDMIEDATSEAIPIMMVGNKADLRQLMAEQGHICVSTNYGEKLSRTYGALFCETSAKEGSNIVEAVLHLAR 662          

HSP 2 Score: 117.087 bits (292), Expect = 2.754e-26
Identity = 132/442 (29.86%), Postives = 208/442 (47.06%), Query Frame = 2
Query:  338 DDLSMRATTLFHTLDPMQRGFINKEDLFSMNCDL--NEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISC--------TSPPVSYKAE--CKLVQCDPAYHKANTCVYTREKIDSVTQDSLPDEHVLIVGDSGLSTLRDVTELESEVE--PVNVF 1621
            D+LS R   LFHT D    G + K    ++  +L  +  +  D+F++LD DKD CI  +DF  G +   + +H    +K   +     S S P + +K   ++ S        R +DE ++     E+    Y  I+   P   +Q+  +I + + + +  S E + L     R +      L  LEEE + +I+ + K+E K ++E+   D + Q + +  E+ ++  K  +  EE  K ++  E+V VL  R   L DL  EN   +  L   QT  + L+SEL  LK    ++    +++   +R +  + +NL +Q++IL+  NRKL D+ND LRSALE   S  +  R + C         SP  SY  E    L  CDP   + N  V           DSLP+  +    DSGLSTLRD  E +SE E  P  +F
Sbjct:    5 DELS-RLRALFHTFDSKSSGRLEKGQFSALCAELKVSPSEAEDIFARLDSDKDSCITFEDFAMGFRGA-RGLHMPEGKKDVEQGEPPKSPSTPDKEEKPEETS-SPAWEDFQRRLADE-VNYIPRREQASILYQNINIVEPLLIQQYEHVIRNFVREIRLQSTEMENLAIAVKRAQDKAAIQLSELEEEMDQRIQAVEKRE-KRKSEEALNDLKRQHESEVAEL-QVTIKKLKKLEEQSKNINLKEDVAVLRRR---LHDLTMENQKLRKDLLEAQTSISFLQSELDALKSDYADQSLSSERDMDIIRGFTDERENLARQIEILQTANRKLHDSNDGLRSALEN--SLMKYNRSLVCLTCSSYLLMSPRSSYLDEDYDSLAVCDP-MQRMNCEV-----------DSLPESCI----DSGLSTLRDSNEYDSEAEYRPPRIF 419          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Xenopus
Match: erich3 (glutamate rich 3 [Source:Xenbase;Acc:XB-GENE-5880720])

HSP 1 Score: 174.096 bits (440), Expect = 6.619e-45
Identity = 88/202 (43.56%), Postives = 126/202 (62.38%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCCK 2632
            +Y++V AGDA  GKS+F+ RLC + F  D   T+G+DF+ K + VDG +  LQ+WDTAGQERFRS+  +YFRKA GVLL+YD T   SF++VR W++ I+ ++   IP++L  NK+D+R     +G +T    +GE LA  +  +F ETSAK G N+++ +  LAR + K  DL+  N      KE +T          CCK
Sbjct:  439 IYRLVLAGDAGSGKSSFLLRLCLNEFRGDIPTTLGVDFQIKKLLVDGDHTTLQIWDTAGQERFRSIAKSYFRKAHGVLLMYDVTSERSFLNVRQWIDEIKNSSDKPIPMMLIGNKTDIRTEL--DGIQT---SMGEKLAMAYSSLFCETSAKDGTNVVEAVLHLAREVKKCTDLEGEN------KEPVTLLSIQDKKANCCK 629          

HSP 2 Score: 60.077 bits (144), Expect = 1.793e-8
Identity = 86/341 (25.22%), Postives = 151/341 (44.28%), Query Frame = 2
Query:  353 RATTLFHTLDPMQRGFINKEDLFSMNCDLN-EDDLLD-VFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPP--QEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFY-DTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDE-EISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSAL 1357
            R  +LF   D    G +  +D  S+  DL  E  L++ +F  LD D DG I+  DF AG + +++ +   NL+          S  PP  +EF+        + R  L + SS       S +E +  FY D    S P   +Q+  ++E++  D +      + L +   R + A    +  +EE+ +  +  + ++    E  ++    + +LQ+K E E   L+ K     +   + L A E+ +  ++ K+Q+ DL +EN   ++ L   QT+ ++L++E+  LK  M +     QQ + N                   E N+ L D NDS+RS L
Sbjct:    9 RLQSLFSACDVRGSGLVELDDFASVCTDLGVEPSLVEPLFRMLDVDGDGIIDFQDFAAGFEEVSQTL---NLQP---------SHQPPAWEEFE-------GRLRGELQHLSS-------STKEWLYGFYQDLQGISVPGLIQQYERLVENVFYDFRAQRLNIEKLESVLKRTEGATAKQIAEMEEDLQHHLTKMERQIQDEELLRLNTAIQ-ELQRKHEMETLSLQTKIDRMTKHKDERL-AMESKEESAKLKNQIFDLSQENEELKMNLLRAQTDISLLQTEVDRLKDYMAD-----QQSHHN------------------SEANKSLYDNNDSMRSTL 298          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Mouse
Match: Rasef (RAS and EF hand domain containing [Source:MGI Symbol;Acc:MGI:2448565])

HSP 1 Score: 194.512 bits (493), Expect = 2.210e-52
Identity = 98/203 (48.28%), Postives = 132/203 (65.02%), Query Frame = 2
Query: 2021 ERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCC 2629
            ++ YKIV AGDA+VGKS+F+ RLC + F  +  AT+G+DF+ KT+ VDG+   LQLWDTAGQERFRS+  +YFRKADGVLLLYD T  +SF++VR+WV+ +       IPI+L  NK+DLRD    E +K +S  LGE LA  +  +F ETSAK G N+++ +  LAR + K  +      I  L   A ++ K S     CC
Sbjct:  354 QKAYKIVLAGDAAVGKSSFLMRLCKNEFQGNTSATLGVDFQMKTLIVDGEQTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNVREWVDMVEDGTHRTIPIMLVGNKADLRDVDNAENQKCISAYLGEKLAMTYGALFCETSAKDGSNVVEAVLHLAREVKKRTEDDDSRSITSL---AGSTSKKSLQMKNCC 553          

HSP 2 Score: 74.7146 bits (182), Expect = 3.150e-13
Identity = 87/284 (30.63%), Postives = 139/284 (48.94%), Query Frame = 2
Query:  821 EKQALLARFHREKLAYENNLRHLEEEYESQIELI---YKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSP--------PVSYKAECKLVQCDPAYHKANTCVYTREKIDSVT-----------QDSLPDEHVLIVGDSGLSTLRDVTELESEVE 1606
            E  A+  +  ++K A +  L  LEEE + +I+ +    +K+ K +AE+   D R Q + +  ++ ++  K  +  EE  + +S  ++V  L   K Q+ DL  EN   + +L   QT    L+SEL  LK    ++    +++   +R Y +D  +L +Q++IL+  NRKL D+ND LRSALE + S      RI+  SP        P       + +  D ++H +    Y  E  DS+             DSLP+       DSGLSTLRD  E +SEV+
Sbjct:    2 ENLAIAVKRAQDKAAIQ--LSELEEEMDQRIQAVENESRKDEKRKAEEALTDLRRQYETEVGDL-QVTIKRLKKLEEQSRQISQKQDVTAL---KKQIHDLTMENQKLKKELLEAQTNVAFLQSELDALKSDYADQSLNSERDLEIIREYTEDRSSLERQIEILQTANRKLHDSNDGLRSALENTYSKLNRSLRINNISPGNTISRSSPKFNHHSSQPLAYDRSFHSS----YADEDCDSLALCDPLQKMNYEVDSLPES----CFDSGLSTLRD-NECDSEVD 270          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Mouse
Match: Rasef (RAS and EF hand domain containing [Source:MGI Symbol;Acc:MGI:2448565])

HSP 1 Score: 194.512 bits (493), Expect = 8.008e-52
Identity = 108/245 (44.08%), Postives = 146/245 (59.59%), Query Frame = 2
Query: 1913 PRGDA------SDSEYPLDKWDSATNLARPSQNQSKQLHYMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCC 2629
            P+G A      S S  P+      T++     N SK      ++ YKIV AGDA+VGKS+F+ RLC + F  +  AT+G+DF+ KT+ VDG+   LQLWDTAGQERFRS+  +YFRKADGVLLLYD T  +SF++VR+WV+ +       IPI+L  NK+DLRD    E +K +S  LGE LA  +  +F ETSAK G N+++ +  LAR + K  +      I  L   A ++ K S     CC
Sbjct:  390 PQGSAGEGSTLSSSRKPISALSLQTDMV---DNTSK---VTSQKAYKIVLAGDAAVGKSSFLMRLCKNEFQGNTSATLGVDFQMKTLIVDGEQTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNVREWVDMVEDGTHRTIPIMLVGNKADLRDVDNAENQKCISAYLGEKLAMTYGALFCETSAKDGSNVVEAVLHLAREVKKRTEDDDSRSITSL---AGSTSKKSLQMKNCC 625          

HSP 2 Score: 83.1889 bits (204), Expect = 7.999e-16
Identity = 94/322 (29.19%), Postives = 152/322 (47.20%), Query Frame = 2
Query:  707 EEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELI---YKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSP--------PVSYKAECKLVQCDPAYHKANTCVYTREKIDSVT-----------QDSLPDEHVLIVGDSGLSTLRDVTELESEVE 1606
            +E++   Y  I+   P   + +  +I + L + K  S E + L     R +      L  LEEE + +I+ +    +K+ K +AE+   D R Q + +  ++ ++  K  +  EE  + +S  ++V  L   K Q+ DL  EN   + +L   QT    L+SEL  LK    ++    +++   +R Y +D  +L +Q++IL+  NRKL D+ND LRSALE + S      RI+  SP        P       + +  D ++H +    Y  E  DS+             DSLP+       DSGLSTLRD  E +SEV+
Sbjct:   34 KEQVSTLYQNITLVEPRLLQPYERVIRNFLREIKLQSTEMENLAIAVKRAQDKAAIQLSELEEEMDQRIQAVENESRKDEKRKAEEALTDLRRQYETEVGDL-QVTIKRLKKLEEQSRQISQKQDVTAL---KKQIHDLTMENQKLKKELLEAQTNVAFLQSELDALKSDYADQSLNSERDLEIIREYTEDRSSLERQIEILQTANRKLHDSNDGLRSALENTYSKLNRSLRINNISPGNTISRSSPKFNHHSSQPLAYDRSFHSS----YADEDCDSLALCDPLQKMNYEVDSLPES----CFDSGLSTLRD-NECDSEVD 342          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Mouse
Match: Rasef (RAS and EF hand domain containing [Source:MGI Symbol;Acc:MGI:2448565])

HSP 1 Score: 192.971 bits (489), Expect = 7.327e-51
Identity = 98/203 (48.28%), Postives = 132/203 (65.02%), Query Frame = 2
Query: 2021 ERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCC 2629
            ++ YKIV AGDA+VGKS+F+ RLC + F  +  AT+G+DF+ KT+ VDG+   LQLWDTAGQERFRS+  +YFRKADGVLLLYD T  +SF++VR+WV+ +       IPI+L  NK+DLRD    E +K +S  LGE LA  +  +F ETSAK G N+++ +  LAR + K  +      I  L   A ++ K S     CC
Sbjct:  507 QKAYKIVLAGDAAVGKSSFLMRLCKNEFQGNTSATLGVDFQMKTLIVDGEQTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNVREWVDMVEDGTHRTIPIMLVGNKADLRDVDNAENQKCISAYLGEKLAMTYGALFCETSAKDGSNVVEAVLHLAREVKKRTEDDDSRSITSL---AGSTSKKSLQMKNCC 706          

HSP 2 Score: 93.9745 bits (232), Expect = 4.634e-19
Identity = 121/444 (27.25%), Postives = 200/444 (45.05%), Query Frame = 2
Query:  353 RATTLFHTLDPMQRGFINKEDLFSMNCDLN--EDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRS-RVNSISSWSPP-QEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELI---YKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSP--------PVSYKAECKLVQCDPAYHKANTCVYTREKIDSVT-----------QDSLPDEHVLIVGDSGLSTLRDVTELESEVE 1606
            R  ++F T D    G + + +  ++  +L+  E +   VF  LD D+DG I   +F  G +     + + + E + +  V    S SP  ++F               + R  DE   L   +E++   Y  I+   P   + +  +I + L + K  S E + L     R +      L  LEEE + +I+ +    +K+ K +AE+   D R Q + +  ++ ++  K  +  EE  + +S  ++V  L   K Q+ DL  EN   + +L   QT    L+SEL  LK    ++    +++   +R Y +D  +L +Q++IL+  NRKL D+ND LRSALE + S      RI+  SP        P       + +  D ++H +    Y  E  DS+             DSLP+       DSGLSTLRD  E +SEV+
Sbjct:   12 RLRSVFATYDTNGSGRLERAEFSALCAELHVAEAEAEAVFRGLDTDRDGAITFPEFARGFR---GALGRDSAEAQGAWEVAEPESPSPAWRDF---------------HARLGDEAQ-LIPRKEQVSTLYQNITLVEPRLLQPYERVIRNFLREIKLQSTEMENLAIAVKRAQDKAAIQLSELEEEMDQRIQAVENESRKDEKRKAEEALTDLRRQYETEVGDL-QVTIKRLKKLEEQSRQISQKQDVTAL---KKQIHDLTMENQKLKKELLEAQTNVAFLQSELDALKSDYADQSLNSERDLEIIREYTEDRSSLERQIEILQTANRKLHDSNDGLRSALENTYSKLNRSLRINNISPGNTISRSSPKFNHHSSQPLAYDRSFHSS----YADEDCDSLALCDPLQKMNYEVDSLPESCF----DSGLSTLRD-NECDSEVD 423          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Mouse
Match: Cracr2a (calcium release activated channel regulator 2A [Source:MGI Symbol;Acc:MGI:2685919])

HSP 1 Score: 184.496 bits (467), Expect = 6.288e-48
Identity = 89/190 (46.84%), Postives = 124/190 (65.26%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEA 2587
            P+RL+KIVF GD++VGK++F+ RLC   F     AT+GID+R KT+ VD   + LQLWDTAGQER+R ++  +FRKADGV ++YD T  +SF+ +R W+ ++ +A G+ IP++L  NK D       E  + V R LGE LA E+ LIF E SA SG N  +++  LAR+L + ED  V N  I +   A
Sbjct:  537 PDRLFKIVFVGDSAVGKTSFLRRLCEARFSPGMAATVGIDYRVKTVTVDNAQVALQLWDTAGQERYRCISQQFFRKADGVAVMYDLTAKQSFLSIRQWLSSVEEAVGDRIPVLLLGNKLD------NEKEREVPRGLGEQLAKENNLIFYECSACSGHNAQESLLHLARLLKEQEDT-VRNDTIQVGPPA 719          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Mouse
Match: Rab44 (RAB44, member RAS oncogene family [Source:MGI Symbol;Acc:MGI:3045302])

HSP 1 Score: 157.532 bits (397), Expect = 1.272e-42
Identity = 83/176 (47.16%), Postives = 109/176 (61.93%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNED 2545
            P+ LY +VF GD++VGK++F+  L  D+F +   AT+G+DFR K + VD K   LQLWDTAGQER+ S+T    RKA+GV+L+YD T  ESF HVR W++ ++ A    + +VL  NK D      EE R+ V  E G  LA E  + F E SA  G NIL+ M  LAR L   ED
Sbjct:   82 PDYLYHVVFLGDSNVGKTSFLHLLHHDAFATGLTATVGVDFRVKNLLVDNKTFALQLWDTAGQERYHSLTRQLLRKAEGVVLMYDVTSQESFTHVRYWLDCLQDAGVEGVAMVLLGNKMD-----CEEERQ-VPTEAGRRLAQELGVSFGECSAALGHNILEPMMNLARSLKMQED 251          
BLAST of ras and EF-hand domain-containing protein homolog vs. UniProt/SwissProt
Match: sp|Q8IZ41|RASEF_HUMAN (Ras and EF-hand domain-containing protein OS=Homo sapiens OX=9606 GN=RASEF PE=1 SV=1)

HSP 1 Score: 207.608 bits (527), Expect = 1.152e-54
Identity = 105/209 (50.24%), Postives = 138/209 (66.03%), Query Frame = 2
Query: 2012 YMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQ---CC 2629
            +  ++ YKIV AGDA+VGKS+F+ RLC + F  +  AT+G+DF+ KT+ VDG+   LQLWDTAGQERFRS+  +YFRKADGVLLLYD T  +SF+++R+WV+ I  AA   +PI+L  NK+D+RD  A EG+K V    GE LA  +  +F ETSAK G NI++ +  LAR + K  D      I +LT        NSK SPQ   CC
Sbjct:  536 FSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTG------TNSKKSPQMKNCC 738          

HSP 2 Score: 99.7525 bits (247), Expect = 5.877e-20
Identity = 123/453 (27.15%), Postives = 195/453 (43.05%), Query Frame = 2
Query:  353 RATTLFHTLDPMQRGFINKEDLFSMNCDLN--EDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFD-------------KTNNSNYSKNRMC--LNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEK---DFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSPPVSY-KAECKLVQCDP---AYHKANTCVYTREKIDSVT-----------QDSLPDEHVLIVGDSGLSTLRDVTELESEVE 1606
            R  ++F   D  + G + +E+  ++  +L     D   VF +LD D+DG I   +F  G  L + +  ++          ++S   P                   T+    S  R       R  DE       EE++   Y  I+   P   + +  +I++ + + +  S E + L     R +      L  LEEE + +I+    K  K E  K E+   D R Q + +  ++ ++  K     EE  K +S  E+V  L   K Q+ DL  EN   +  L   QT    L+SEL  LK    ++    +++   +R+Y +D  +L +Q++IL+  NRKL D+ND LRSALE S S       I+  SP  +  ++  K +   P    Y +++   Y  E  DS+             DSLP+       DSGLSTLRD  E +SEVE
Sbjct:   12 RLRSVFAACDANRSGRLEREEFRALCTELRVRPADAEAVFQRLDADRDGAITFQEFARGF-LGSLRGGRRRDWGPLDPAPAVSEAGPETHDSEEDEGDEDAAAALATSCGPASPGRAWQDFQARLGDEA-KFIPREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALSDLRRQYETEVGDL-QVTIKKLRKLEEQSKRVSQKEDVAAL---KKQIYDLSMENQKVKKDLLEAQTNIAFLQSELDALKSDYADQSLNTERDLEIIRAYTEDRNSLERQIEILQTANRKLHDSNDGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFIGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCF----DSGLSTLRDPNEYDSEVE 454          
BLAST of ras and EF-hand domain-containing protein homolog vs. UniProt/SwissProt
Match: sp|Q5RI75|RASEF_MOUSE (Ras and EF-hand domain-containing protein homolog OS=Mus musculus OX=10090 GN=Rasef PE=1 SV=1)

HSP 1 Score: 194.512 bits (493), Expect = 5.602e-51
Identity = 108/245 (44.08%), Postives = 146/245 (59.59%), Query Frame = 2
Query: 1913 PRGDA------SDSEYPLDKWDSATNLARPSQNQSKQLHYMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCC 2629
            P+G A      S S  P+      T++     N SK      ++ YKIV AGDA+VGKS+F+ RLC + F  +  AT+G+DF+ KT+ VDG+   LQLWDTAGQERFRS+  +YFRKADGVLLLYD T  +SF++VR+WV+ +       IPI+L  NK+DLRD    E +K +S  LGE LA  +  +F ETSAK G N+++ +  LAR + K  +      I  L   A ++ K S     CC
Sbjct:  390 PQGSAGEGSTLSSSRKPISALSLQTDMV---DNTSK---VTSQKAYKIVLAGDAAVGKSSFLMRLCKNEFQGNTSATLGVDFQMKTLIVDGEQTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNVREWVDMVEDGTHRTIPIMLVGNKADLRDVDNAENQKCISAYLGEKLAMTYGALFCETSAKDGSNVVEAVLHLAREVKKRTEDDDSRSITSL---AGSTSKKSLQMKNCC 625          

HSP 2 Score: 83.1889 bits (204), Expect = 5.595e-15
Identity = 94/322 (29.19%), Postives = 152/322 (47.20%), Query Frame = 2
Query:  707 EEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELI---YKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSP--------PVSYKAECKLVQCDPAYHKANTCVYTREKIDSVT-----------QDSLPDEHVLIVGDSGLSTLRDVTELESEVE 1606
            +E++   Y  I+   P   + +  +I + L + K  S E + L     R +      L  LEEE + +I+ +    +K+ K +AE+   D R Q + +  ++ ++  K  +  EE  + +S  ++V  L   K Q+ DL  EN   + +L   QT    L+SEL  LK    ++    +++   +R Y +D  +L +Q++IL+  NRKL D+ND LRSALE + S      RI+  SP        P       + +  D ++H +    Y  E  DS+             DSLP+       DSGLSTLRD  E +SEV+
Sbjct:   34 KEQVSTLYQNITLVEPRLLQPYERVIRNFLREIKLQSTEMENLAIAVKRAQDKAAIQLSELEEEMDQRIQAVENESRKDEKRKAEEALTDLRRQYETEVGDL-QVTIKRLKKLEEQSRQISQKQDVTAL---KKQIHDLTMENQKLKKELLEAQTNVAFLQSELDALKSDYADQSLNSERDLEIIREYTEDRSSLERQIEILQTANRKLHDSNDGLRSALENTYSKLNRSLRINNISPGNTISRSSPKFNHHSSQPLAYDRSFHSS----YADEDCDSLALCDPLQKMNYEVDSLPES----CFDSGLSTLRD-NECDSEVD 342          
BLAST of ras and EF-hand domain-containing protein homolog vs. UniProt/SwissProt
Match: sp|Q08CX1|RASEF_XENTR (Ras and EF-hand domain-containing protein OS=Xenopus tropicalis OX=8364 GN=rasef PE=2 SV=1)

HSP 1 Score: 194.512 bits (493), Expect = 1.976e-50
Identity = 94/178 (52.81%), Postives = 125/178 (70.22%), Query Frame = 2
Query: 2021 ERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAK---NED 2545
            E+ YKIV AGDA+VGKS+F+ RLC + F  +  AT+G+DF+ KT+ VDG+   LQLWDTAGQERFRS+  +YFR+ADGVLLLYD T  +SF++VR+W++ I  A    IPI++  NK+DLR   AE+G   VS   GE L+  +  +F ETSAK G NI++ +  LAR + K   NED
Sbjct:  520 EKAYKIVLAGDAAVGKSSFLMRLCKNEFRGNTSATLGVDFQMKTLVVDGEPTILQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCEKSFLNVREWIDMIEDATSEAIPIMMVGNKADLRQLMAEQGHICVSTNYGEKLSRTYGALFCETSAKEGSNIVEAVLHLAREVRKRCDNED 697          

HSP 2 Score: 114.005 bits (284), Expect = 2.024e-24
Identity = 128/446 (28.70%), Postives = 209/446 (46.86%), Query Frame = 2
Query:  338 DDLSMRATTLFHTLDPMQRGFINKEDLFSMNCDL--NEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXV--LSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSPPVSY-KAECKLVQCDPAYHKANTCVYTREKIDSVT-----------QDSLPDEHVLIVGDSGLSTLRDVTELESEVE--PVNVF 1621
            D+LS R   LFHT D    G + K    ++  +L  +  +  D+F++LD DKD CI  +DF  G +   + +H    +K   +     S S P + +K   ++ S        R +DE ++     E+    Y  I+   P   +Q+  +I + + + +  S E + L     R +      L  LEEE + +I+ + K+  K E  K E+      ++ + E++ L+   K+ ++  ++   ++  E+V VL  R   L DL  EN   +  L   QT  + L+SEL  LK    ++    +++   +R +  + +NL +Q++IL+  NRKL D+ND LRSALE S        R   TSP  +  +   KL +C   Y ++    Y  E  DS+             DSLP+  +    DSGLSTLRD  E +SE E  P  +F
Sbjct:    5 DELS-RLRALFHTFDSKSSGRLEKGQFSALCAELKVSPSEAEDIFARLDSDKDSCITFEDFAMGFRGA-RGLHMPEGKKDVEQGEPPKSPSTPDKEEKPEETS-SPAWEDFQRRLADE-VNYIPRREQASILYQNINIVEPLLIQQYEHVIRNFVREIRLQSTEMENLAIAVKRAQDKAAIQLSELEEEMDQRIQAVEKRVKKEEKRKSEEALNDLKRQHESEVAELQVTIKKLKKLEEQSKNINLKEDVAVLRRR---LHDLTMENQKLRKDLLEAQTSISFLQSELDALKSDYADQSLSSERDMDIIRGFTDERENLARQIEILQTANRKLHDSNDGLRSALENSLMKYNRSLRTINTSPGSTISRNSPKLTRCTSPYDRSPRSSYLDEDYDSLAVCDPMQRMNCEVDSLPESCI----DSGLSTLRDSNEYDSEAEYRPPRIF 439          
BLAST of ras and EF-hand domain-containing protein homolog vs. UniProt/SwissProt
Match: sp|Q22908|RASEF_CAEEL (Ras and EF-hand domain-containing protein homolog OS=Caenorhabditis elegans OX=6239 GN=rsef-1 PE=2 SV=2)

HSP 1 Score: 174.481 bits (441), Expect = 3.565e-44
Identity = 84/189 (44.44%), Postives = 124/189 (65.61%), Query Frame = 2
Query: 2021 ERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQ------VMNPII 2569
            ER ++IV  GDA+VGKS+F+ R+    F +   +T+G+DF  KT+ VDG+ + LQLWDTAGQERFRS+  +YFR+ADG +L+YD    +SF+ VRDW+ETI+++    IPI+L  NK D+  R +  G  +V++  G ++A+   ++F+ETSA  G NI   M  L R L   ED++      V+NP +
Sbjct:  459 ERTFRIVMCGDAAVGKSSFVMRVIRRQFTNQLPSTLGVDFHVKTVNVDGRNVALQLWDTAGQERFRSLCKSYFRRADGAILVYDVCAEQSFLRVRDWIETIKESTERSIPIILVGNKVDM--RISTPG--SVAKTDGASMAAAMGVLFMETSALDGSNIDNAMLALTRELMAVEDVEIRSTGVVLNPAV 643          

HSP 2 Score: 96.2857 bits (238), Expect = 5.329e-19
Identity = 98/381 (25.72%), Postives = 184/381 (48.29%), Query Frame = 2
Query:  365 LFHTLDPMQRGFINKEDLFSMNCDLNEDDLLDVFSQLDQDKDGCINVDDFKAGLK--LINKQIHKKNLEKRRSRVNSISSWSPP------QEFDKTNNSNYSKNRMCL-----NNRSSDEGMSL-FS--CEEKICDFYDTISKSS-PDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRL--------QQENINLRSYE-------KDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSP 1411
            LF   D   +G++  EDL  +   L+++DL  +F++LDQD  G I   +F  G +  + + + H  N  +RR+   S++   PP      Q FD  ++S  S+  + +      +  SD  +++ FS  C+E++   Y+ +  S  P    +F  ++ S   +  E   E + L   +  E+  Y   +  +E E + Q+EL   K  + E +++ K+     Q+  +E+S ++   +  ++  K +    E +    E  D+L  +  ENN+ +  L+    E  M++SEL  ++ + + K   L        Q E IN  S         ++ +++ KQLQ+L + NRKL +TN+SLR AL++  S   ++R+ +  +P
Sbjct:    9 LFSLCDSESKGYLTMEDLRKVCPQLDDNDLRFIFTELDQDGSGKIEKLEFLRGFQDTVQHGESHGLNGMQRRA---SVAVEDPPMFLRDEQMFDSESDSTSSRPAIRVFDEEHYHSESDTNINIDFSVPCQEEVLVLYEQLQSSGVPALLRKFERVVGSFHKELSEKKHENERLQRIYASEREMYNRRMEEMESEVDQQLELTEMKARQEERDRLTKEKEEMRQRMSDEMSEMRNNIERLQKMEKALERENERLNHQKELSDKLKVVNEENNDLRQNLAENHLELAMIKSELAQVRCEFDQKQDELSARRGILFQLEPINSLSSSDQASHATEESESVRKQLQLLFDANRKLHETNESLRDALDSRAS---VLRQFNLRTP 383          
BLAST of ras and EF-hand domain-containing protein homolog vs. UniProt/SwissProt
Match: sp|Q619T5|RASEF_CAEBR (Ras and EF-hand domain-containing protein homolog OS=Caenorhabditis briggsae OX=6238 GN=rsef-1 PE=3 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 1.636e-43
Identity = 83/187 (44.39%), Postives = 120/187 (64.17%), Query Frame = 2
Query: 2021 ERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQ------VMNP 2563
            ER ++IV  GDA+VGKS+F+ R+    F +   +T+G+DF  KT+ VDG+ + LQLWDTAGQERFRS+  +YFR+ADG +L+YD     SF+ VRDW+ETI+++    IPI+L  NK D+R +        V++  G ++A+   ++F+ETSA  G NI   M  L R L   ED++      V+NP
Sbjct:  437 ERTFRIVMCGDAAVGKSSFVMRVIRRQFTNQLPSTLGVDFHVKTVNVDGRNVALQLWDTAGQERFRSLCKSYFRRADGAILVYDVCAEHSFLRVRDWIETIKESTERSIPIILVGNKVDMRLQTP----GAVAKTDGASMAAAMGVLFMETSALDGSNIDNAMLALTRELMAVEDVEIRSTGVVLNP 619          

HSP 2 Score: 82.4185 bits (202), Expect = 1.202e-14
Identity = 90/364 (24.73%), Postives = 172/364 (47.25%), Query Frame = 2
Query:  365 LFHTLDPMQRGFINKEDLFSMNCDLNEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLE--KRRSRVNSISSWSPPQEFDKT--NNSNYSKNRMCLN-------NRSSDEGMSL-FS--CEEKICDFYDTISKSS-PDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSP 1411
            LF   D   +GF+  EDL  +   L+++DL  +F++LD+D  G I   +F  G +   +    + L   +RR+ V +     P    D+    + + S +R  +        +  SD  +++ FS  C+E++   Y+ +  S  P    +F  ++ S   +  E   E + L   +  E+  Y   +  +E E + Q+ELI  K  + E E++ K+     ++  EE+S ++   +  +   K +    E +    +  D+L  +  ENN+ +  L+    E  M++SEL  ++   + K   L           ++ +++ KQLQ+L + NRKL +TN+SLR AL++  S   ++R+ +  +P
Sbjct:    9 LFSLCDSESKGFLTMEDLKKVCPQLDDNDLRFIFNELDRDGSGKIEKMEFLQGFQETVQHGESRGLNGMQRRASV-AFDDGGPVFRRDELVFESESDSSSRPAIRVYDEEHYHSESDTNINIDFSVPCQEEVLVLYEQLQSSGVPALLRKFERVVGSFHKELSEKKHENERLQRIYASEREMYNRRMEEMESEVDQQLELIEMKARQEERERLTKEKEEMRERMSEEMSEMRTNIERLQRMEKVLERENERLNHQKDLSDKLKVVNEENNDLRQNLAENHLELAMIKSELAQVRADFDQKQDELSARRDQASHATEESESVRKQLQLLFDANRKLHETNESLRDALDSRAS---VLRQFNLRTP 368          
BLAST of ras and EF-hand domain-containing protein homolog vs. TrEMBL
Match: A0A2C9LF24 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106072326 PE=4 SV=1)

HSP 1 Score: 299.286 bits (765), Expect = 3.206e-84
Identity = 242/799 (30.29%), Postives = 394/799 (49.31%), Query Frame = 2
Query:  365 LFHTLDPMQRGFINKEDLFSMNCDLNEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTI-SKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKD---FRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCT--------SPPVSYKAECKLVQCDP----------AYHKANTCVYTREKIDSVTQDSLPDEHVLIVGDSGLSTLRDVTEL--ESEVEPVNVFSEVCVRQCKFGTSLKSLDKIDDLVNDCQTQSNDKTMVSRPNSLQTXXXXXXXXXXXXXLHQKS-DVGELSGNSAQGTSCRQKGIKEKRKYQSKYRD--RYPRGDASDSEYPLDKWDSATNLARPSQNQSKQLHYMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGI---DFRNKTIEVDG--------KYIGLQLWDTAGQ-----ERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCCK 2632
            LFH  D    G+I++E+L S+  +L+ +DL DVF QLD+D DG I++++F  G + I      K  +  R R+ S  S     +    +   Y+           DE +   SC+E++C+ Y  + S   P+   QF  I+ +++ND ++   E + L   + REK  ++ +LR LEEE E Q++ I ++  K E E++E +    R QL   D EI  L++  K+ + E +  L   +   +L+ +K +L ++  EN   + +L+  QT   ++RS++ T+KQQ++ K   L+ E   +  Y  +   L +QLQ+L E N+ L DTND+LR  L++ P+ S   +R + T            S K+   L    P            H  N  +     +     +S  D+++    DSG ST+RD+ ++  ++E++ V+V S++   +           ++ +   D   + +D T V+RP SL+T +  +         H++S  VG    N +Q +  R + I  +    S+     + P         P+  + S T +++            PER+YKIV AGDA+VGKS+FI RLC   FV +  +T+G     F  K + V          +   L  + +  +      +FRS+   YFR+ADGVLLLYD TY  SF+++RDWV+ I + A  +IPI+L  NK D R     +GR+ V  + G  L+ E   +F+ETSAK G NI + + EL R+L  NEDL+V    + L       +   K S  CCK
Sbjct:   13 LFHACDLDGSGYIDQEELASICSELSLEDLGDVFKQLDKDGDGRISIEEFAKGYREIAMAKETKKRQLIRQRLASAKSDENLADGGNEDAEEYAS--------GLDEALKALSCQEQVCELYQNLHSVDKPELVSQFESILLNVINDVRQYQLENERLEKTYRREKDEHDKHLRRLEEEMEQQVQSIEERVRKQEKERLESEKIELRNQL---DAEIIMLQQNLKKLQREGENRLDKGQEEHLLNLKK-RLEEIGSENRMLKSELTDAQTNLALIRSQMTTVKQQLDEKNYELEIETKTIEDYVNEQDKLRRQLQMLHEANKTLLDTNDNLREMLDSVPNGSPGFKRTASTDFELGALYKSGASAKSNRSLSPNFPRKGSVMSDYVEVHPGNRPLLESGMMPRFLSESCEDDNIAEDLDSGHSTMRDMNDVNDDTELDSVSVQSDMRRSRRHKRLRTGM--RVQEEEPDSHDEESD-TEVTRPGSLETFRLPND--------HKRSPSVGSRGSNRSQKSLNRDESILSQSSKSSRGSSRRQLP-------AVPIKDFTSPTKVSKE-----------PERMYKIVLAGDAAVGKSSFIVRLCKGKFVPNLSSTLGYLSYAFEAKIVTVRATPTLLCLARVFFLTFFVSKAETEEYGRKFRSVARTYFRRADGVLLLYDVTYERSFLNLRDWVDAIEEGALKKIPIMLVGNKIDARPELEHQGRRVVKHDDGVRLSREIDALFIETSAKDGANIHEAVIELTRLLRTNEDLEVKTVGMQL------HEMKDKKSGSCCK 764          
BLAST of ras and EF-hand domain-containing protein homolog vs. TrEMBL
Match: A0A0D9R5K6 (RAS and EF-hand domain containing OS=Chlorocebus sabaeus OX=60711 GN=RASEF PE=4 SV=1)

HSP 1 Score: 281.952 bits (720), Expect = 3.891e-78
Identity = 249/794 (31.36%), Postives = 363/794 (45.72%), Query Frame = 2
Query:  353 RATTLFHTLDPMQRGFINKEDLFSMNCDLN--EDDLLDVFSQLDQDKDGCINVDDFKAGLK--LINKQIHKKNLEKRRSRVNSISSWSPPQEFDK-------TNNSNYSKNRMCLNNRSS-DEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEK---DFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSPPVSY-KAECKLVQCDP---AYHKANTCVYTREKIDSVT-----------QDSLPDEHVLIVGDSGLSTLRDVTEL--ESEVEPVNVFSEVCVRQCKFGTSLKSLDKIDDLVNDCQTQSNDKTMVSRPNSLQTXXXXXXXXXXXXXLHQKSDVGELSGNSAQGTSCRQKGIKEKRKYQSKYRDRYPRGDASDSEYPLDKWDSATNLARPSQNQSKQLHYMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQ---CC 2629
            R  ++F   D  + G + +E+  S+  +L     D   VF +LD D DG I   +F  G +  L   +   + L +    V      +P  E D+       T+    S +R   + ++   E       EE++   Y  I+   P   + +  +I++ + + +  S E + L     R +      L  LEEE + +I+    K  K E  K E+   D R Q + +  ++ ++  K     EE  K +S  E+V  L   K Q+ DL  EN   + +L   QT    L+SEL  LK    ++    +++   +R Y +D  +L +Q++IL+  NRKL D+ND LRSALE S S      RI+  SP  +  ++  K     P    Y +++   Y  E  DS+             DSLP+       DSGLSTLRD  E   E E +    F      Q  FG      D  D  + D +T                                  DV  +S    QGT      +   RK                   P+      T+L     + +K   +  ++ YKIV AGDA+VGKS+F+ RLC + F  +  AT+G+DF+ KT+ VDG+   LQLWDTAGQERFRS+  +YFRKADGVLLLYD T  +SF+++R+WV+ I  AA   +PI+L  NK+D+RD  A EG+K V    GE LA  +  +F ETSAK G NI++ +  LAR + K  D      I +LT        NSK SPQ   CC
Sbjct:   12 RLRSVFAACDANRSGRLEREEFESLCAELRVRPADAETVFQRLDADHDGAITFQEFARGFRGALRGGRRQDRGLLEPAPAVAEAGPETPDSEEDEDAEAAQATSWGPASPDRAWQDFQARLGEEAKFIPREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALSDLRRQYETEVGDL-QVTIKKLRKLEEQSKHISQKEDVAAL---KKQIYDLSMENQKVKKELLEAQTNIAFLQSELDALKSDYADQSLNTERDLEIIRGYTEDRNSLERQIEILQTANRKLHDSNDGLRSALENSYSKFNRSLRINNISPGNTISRSSPKFTGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCF----DSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDVPD--IRDEET------------------------------FGLEDVASISDWKPQGTVSEGSIVSSSRK-------------------PISALSPQTDLV---DDNAKS--FSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADVRDTAAAEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSKSITNLTG------TNSKKSPQMKNCC 735          
BLAST of ras and EF-hand domain-containing protein homolog vs. TrEMBL
Match: A0A096P1V3 (RAS and EF-hand domain containing OS=Papio anubis OX=9555 GN=RASEF PE=4 SV=1)

HSP 1 Score: 274.633 bits (701), Expect = 1.551e-75
Identity = 247/797 (30.99%), Postives = 357/797 (44.79%), Query Frame = 2
Query:  353 RATTLFHTLDPMQRGFINKEDLFSMNCDLN--EDDLLDVFSQLDQDKDGCINVDDFKAGLK--LINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSC-----------EEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEK---DFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSPPVSY-KAECKLVQCDP---AYHKANTCVYTREKIDSVT-----------QDSLPDEHVLIVGDSGLSTLRDVTEL--ESEVEPVNVFSEVCVRQCKFGTSLKSLDKIDDLVNDCQTQSNDKTMVSRPNSLQTXXXXXXXXXXXXXLHQKSDVGELSGNSAQGTSCRQKGIKEKRKYQSKYRDRYPRGDASDSEYPLDKWDSATNLARPSQNQSKQLHYMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQ---CC 2629
            R  ++F   D  + G + +E+  S+  +L     D   VF +LD D DG I   +F  G +  L   +   + L      V      +P  E D+ +    +         S D     F             EE++   Y  I+   P   + +  +I++ + + +  S E + L     R +      L  LEEE + +I+    K  K E  K E+   D R Q + +  ++ ++  K     EE  K +S  E+V  L   K Q+ DL  EN   + +L   QT    L+SEL  LK    ++    +++   +R Y +D  +L +Q++IL+  NRKL D+ND LRSALE S S      RI+  SP  +  ++  K     P    Y +++   Y  E  DS+             DSLP+       DSGLSTLRD  E   E E +    F      Q  FG      D  D  + D +T                                  DV  +S    QGT      +   RK                   P+      T+L     + +K   +  ++ YKIV AGDA+VGKS+F+ RLC + F  +  AT+G+DF+ KT+ VDG+   LQLWDTAGQERFRS+  +YFRKADGVLLLYD T  +SF+++R+WV+ I  AA   +PI+L  NK+D+RD  A  G+K V    GE LA  +  +F ETSAK G NI++ +  LAR + K  D      I +LT        NSK SPQ   CC
Sbjct:   12 RLRSVFAACDANRSGRLEREEFGSLCAELRVRPADAEAVFQRLDADHDGAITFQEFARGFRGALRGGRREDRGLLGPAPAVAEAGPETPDSEEDEGDEDAEAALATSWGPASPDPAWQDFQARLGEEAKFIPREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALSDLRRQYETEVGDL-QVTIKKLRKLEEQSKHISQKEDVAAL---KKQIYDLSMENQKVKKELLEAQTNIAFLQSELDALKSDYADQSLNTERDLEIIRGYTEDRNSLERQIEILQTANRKLHDSNDGLRSALENSYSKFNRSLRINNISPGNTISRSSPKFTGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCF----DSGLSTLRDPNEYDSEVEYKHQRGFQRPHGVQESFGGDASDTDVPD--IRDEET------------------------------FGLEDVASISDWKLQGTVSEGSIVSSSRK-------------------PISALSPQTDLV---DDNAKS--FSTQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADVRDTAAAGGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSKSITNLTG------TNSKKSPQMKNCC 738          
BLAST of ras and EF-hand domain-containing protein homolog vs. TrEMBL
Match: A0A2K5WTK6 (RAS and EF-hand domain containing OS=Macaca fascicularis OX=9541 GN=RASEF PE=4 SV=1)

HSP 1 Score: 274.633 bits (701), Expect = 1.598e-75
Identity = 248/797 (31.12%), Postives = 360/797 (45.17%), Query Frame = 2
Query:  353 RATTLFHTLDPMQRGFINKEDLFSMNCDLN--EDDLLDVFSQLDQDKDGCINVDDFKAGLK--LINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSC-----------EEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELI---YKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSPPVSY-KAECKLVQCDP---AYHKANTCVYTREKIDSVT-----------QDSLPDEHVLIVGDSGLSTLRDVTEL--ESEVEPVNVFSEVCVRQCKFGTSLKSLDKIDDLVNDCQTQSNDKTMVSRPNSLQTXXXXXXXXXXXXXLHQKSDVGELSGNSAQGTSCRQKGIKEKRKYQSKYRDRYPRGDASDSEYPLDKWDSATNLARPSQNQSKQLHYMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQ---CC 2629
            R  ++F   D  + G + +E+  S+  +L     D   VF +LD D DG I   +F  G +  L   +     L      V      +P  E D+ +    +         S D     F             EE++   Y  I+   P   + +  +I++ + + +  S E + L     R +      L  LEEE + +I+      +K+ K +AE+   D R Q + +  ++ ++  K     EE  K +S  E+V  L   K Q+ DL  EN   + +L   QT    L+SEL  LK    ++    +++   +R Y +D  +L +Q++IL+  NRKL D+ND LRSALE S S      RI+  SP  +  ++  K     P    Y +++   Y  E  DS+             DSLP+       DSGLSTLRD  E   E E +    F      Q  FG      D  D  + D +T                                  DV  +S    QGT      +   RK  S      P+ D  D        D+A +             +  ++ YKIV AGDA+VGKS+F+ RLC + F  +  AT+G+DF+ KT+ VDG+   LQLWDTAGQERFRS+  +YFRKADGVLLLYD T  +SF+++R+WV+ I  AA   +PI+L  NK+D+RD  A EG+K V    GE LA  +  +F ETSAK G NI++ +  LAR + K  D      + +LT        NSK SPQ   CC
Sbjct:   12 RLRSVFAAYDANRSGRLEREEFGSLCAELRVRPADAEAVFQRLDADHDGAITFQEFARGFRGALRGGRRQDWGLLGPALAVAEAGPETPDSEEDEGDEDAEAALATSWGPASPDPAWQDFQARLGEEAKFIPREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHQTRKDEKRKAEEALSDLRRQYETEVGDL-QVTIKKLRKLEEQSKHISQKEDVAAL---KKQIYDLSMENQKVKKELLEAQTNIAFLQSELDALKSDYADQSLNTERDLEIIRGYTEDRNSLERQIEILQTANRKLHDSNDGLRSALENSYSKFNRSLRINNISPGNTISRSSPKFTGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCF----DSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDVPD--IRDEET------------------------------FGLEDVASISDWKLQGTVSEGSIVSSSRKPISALS---PQTDLVD--------DNAKS-------------FSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAAAEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSKSVTNLTG------TNSKKSPQMKNCC 738          
BLAST of ras and EF-hand domain-containing protein homolog vs. TrEMBL
Match: G7NGE5 (Ras-related protein Rab-45 OS=Macaca mulatta OX=9544 GN=EGK_07816 PE=4 SV=1)

HSP 1 Score: 273.478 bits (698), Expect = 3.567e-75
Identity = 244/797 (30.61%), Postives = 359/797 (45.04%), Query Frame = 2
Query:  353 RATTLFHTLDPMQRGFINKEDLFSMNCDLN--EDDLLDVFSQLDQDKDGCINVDDFKAGLK-LINKQIHKK-NLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSC-----------EEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELI---YKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSPPVSY-KAECKLVQCDP---AYHKANTCVYTREKIDSVT-----------QDSLPDEHVLIVGDSGLSTLRDVTEL--ESEVEPVNVFSEVCVRQCKFGTSLKSLDKIDDLVNDCQTQSNDKTMVSRPNSLQTXXXXXXXXXXXXXLHQKSDVGELSGNSAQGTSCRQKGIKEKRKYQSKYRDRYPRGDASDSEYPLDKWDSATNLARPSQNQSKQLHYMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQ---CC 2629
            R  ++F   D  + G + +E+  S+  +L     D   VF +LD D DG I   +F  G +  + +  H+   L      V      +P  E D+ +    +         S D     F             EE++   Y  I+   P   + +  +I++ + + +  S E + L     R +      L  LEEE + +I+      +K+ K +AE+   D R Q + +  ++ ++  K     EE  K +S  E+V  L   K Q+ DL  EN   + +L   QT    L+SEL  LK    ++    +++   +R Y +D  +L +Q++IL+  NRKL D+ND LRSALE S S      RI+  SP  +  ++  K     P    Y +++   Y  E  DS+             DSLP+       DSGLSTLRD  E   E E +    F      Q  FG      D  D  + D +T                                  DV  +S    QGT      +   RK                   P+      T+L   +        +  ++ YKIV AGDA+VGKS+F+ RLC + F  +  AT+G+DF+ KT+ VDG+   LQLWDTAGQERFRS+  +YFRKADGVLLLYD T  +SF+++R+WV+ I  AA   +PI+L  NK+D+RD  A EG+K V    GE LA  +  +F ETSAK G NI++ +  LAR + K  D      + +LT        NS+ SPQ   CC
Sbjct:   12 RLRSVFAAYDANRSGRLEREEFGSLCAELRVRPADAEAVFQRLDADHDGAITFQEFARGFRGALRRGRHQDWGLLGSALAVAEAGPETPDSEEDEGDEDAEAALATSWGPASPDPAWQDFQARLGEEAKFIPREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHQTRKDEKRKAEEALSDLRRQYETEVGDL-QVTIKKLRKLEEQSKHISQKEDVAAL---KKQIYDLSMENQKVKKELLEAQTNIAFLQSELDALKSDYADQSLNTERDLEIIRGYTEDRNSLERQIEILQTANRKLHDSNDGLRSALENSYSKFNRSLRINNISPGNTISRSSPKFTGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCF----DSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDVPD--IRDEET------------------------------FGLEDVASISDWKLQGTVSEGSIVSSSRK-------------------PISALSPQTDLVDDNAKS-----FSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAAAEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSKSVTNLTG------TNSRKSPQMKNCC 738          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Cavefish
Match: rasef (RAS and EF-hand domain containing [Source:NCBI gene;Acc:103039240])

HSP 1 Score: 189.119 bits (479), Expect = 1.519e-49
Identity = 88/173 (50.87%), Postives = 123/173 (71.10%), Query Frame = 2
Query: 2021 ERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKN 2539
            ++ YKIV AGDA+VGKS+F+ RLC + F     AT+G+DF+ KT+ VDG    LQLWDTAGQERFRS+  +YFR+ADGVLLLYD T  +SF++VR+WV+ I   + +++PI+L  NK+DLR++  +EG   +    GE LA  +  +F ETSAK G NI++ +  LAR + K+
Sbjct:  523 DKTYKIVLAGDAAVGKSSFLLRLCKNEFKGTTSATLGVDFQMKTLVVDGVPTVLQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCEKSFLNVREWVDIIEDVSQDDVPIMLVGNKTDLREQALQEGITCIPTSYGEKLAMTYSSLFCETSAKEGSNIVEAVLHLAREVIKH 695          

HSP 2 Score: 77.0258 bits (188), Expect = 6.344e-14
Identity = 105/447 (23.49%), Postives = 188/447 (42.06%), Query Frame = 2
Query:  362 TLFHTLDPMQRGFINKEDLFSMNCDLN--EDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQ-------LSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPS---------ASEIIRRISCTSPPVSYKAECKLVQCDPAYHKANTCVYT-REKIDSVT-------------QDSLPDEHVLIVGDSGLSTLRDVTELESEVE 1606
            +LF + D    G I K + F +  +L    +D   +FS+LD D+DG IN+DDF  G +        +N  KR   V             +  +  +   R  L      E        +++   Y  IS +      Q+  ++ + + + K  + E + L     R +      L  +EEE + +I    +     E++K E       Q  + +++ L++K +        +L  +E+    +  +++   L+R+NN   +Q       L    T    L+SEL ++K  + ++    +++   +R + ++  NL +Q+++L+  NRKL D+ND LR+ LE S +          S + RR S  +P   Y   C  +  +  Y +     +  R+  DS+               DSLP+  V    DSG+STLR     +SE E
Sbjct:   24 SLFRSCDSDNSGRIEKGEFFRLCSELQVRTEDAHAIFSKLDMDQDGAINLDDFVKGFQT------AENFMKREEEVEEEEEMEEQSRGGEAFSLAWEDFRSRLG-----EQQKFIPRIDQVSILYQNISLTEARIIPQYERVVLNFIREIKLQNTEMEHLALAVKRAQDQATIQLSEMEEEMDQRIHATERNTRIKESKKAETALTSMKQHYEAQLTELQQKIR--------ILQMSEDQNKNTSLQEENAALKRKNNELTLQNQKLKQELLECHTNVAFLQSELDSIKSDLTDQSINFERDEALMRCFAEERDNLERQIEMLQAANRKLHDSNDGLRATLENSQAKNKGQRLSPGSTMARRKSLCTP---YFERCCNMCGEDDYDQLQVMEHLRRQSCDSLALALCDPMRRHSFELDSLPESCV----DSGMSTLRSNNGYDSEQE 444          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Cavefish
Match: zgc:162879 (ras and EF-hand domain-containing protein-like [Source:NCBI gene;Acc:103037315])

HSP 1 Score: 167.162 bits (422), Expect = 1.249e-42
Identity = 81/171 (47.37%), Postives = 112/171 (65.50%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKN 2539
            +Y++V AGDA  GKS+F+ RL  + F  D   T+G+DF+ K + VDG+   LQ+WDTAGQERFRS+  +YFRKA GVLLLYD T   SF++VR+W+E I+++    +P+ +  NK D+R    E     VS   GE LA  +  +F ETSAK G NI++ +  LAR + KN
Sbjct:  474 MYRLVLAGDAGSGKSSFLLRLSLNEFRGDMQTTLGVDFQMKKMLVDGEKTNLQIWDTAGQERFRSIARSYFRKAHGVLLLYDVTSESSFLNVREWMEQIQESTDGNVPMCVIGNKVDMRAERPEGS--CVSPAQGEKLAMTYNALFCETSAKEGTNIVEAVLHLAREVKKN 642          

HSP 2 Score: 74.7146 bits (182), Expect = 3.279e-13
Identity = 88/365 (24.11%), Postives = 170/365 (46.58%), Query Frame = 2
Query:  365 LFHTLDPMQRGFINKEDLFSMNCDLN--EDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRA-EQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKD------QLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTS--PPVSYK 1426
            LF   D  + G I  ED   +  +L+    D+  +FS+ D D DGCIN  DF +    +++ ++  +L    S     S+W+   EF+              N    D    L    + + + Y  I  +S     +Q+ D+I++++ +++E   E + L     R +    + +  LEE+ + Q+    ++  + E +K+E    M   K + E++ L+   +        +    E  ++ S R+D      ++ ++ +EN+  +  L   Q   ++L+ EL  LK    ++  + ++E+  L+    ++Q+    ++ L+E+N+KL D+ND LRSAL T  S+S+  RR+S  +  PP   K
Sbjct:    9 LFSACDGNKSGRIEYEDFIRVCRELSVPAGDVRTLFSKFDVDGDGCINYSDFCSSFNEVSETLNLASLGS--SGQCQASAWA---EFE--------------NTLDGDVAYYLGRLGDPLGELYQEIHATSDMLLLQQYEDLIKALVLESREHRMESEQLETSLRRSEETASSQIVELEEDLQRQLTRTEQRVREEEQKKMEGTLAMLQIKHENELADLQAAVER-------LTEYQEETELNSPREDLIKLRKKIREVTQENDELRSSLLKAQMNISVLQVELDKLKNAYTDEKLQHERESDELKKMVVEYQSYAGHIEFLQEMNKKLYDSNDGLRSAL-TKDSSSK--RRLSPKNEKPPRKLK 344          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Cavefish
Match: rab13 (RAB13, member RAS oncogene family [Source:NCBI gene;Acc:103044636])

HSP 1 Score: 153.68 bits (387), Expect = 4.818e-42
Identity = 74/166 (44.58%), Postives = 115/166 (69.28%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELAR 2524
            L+K++  GD+ VGK+  I R   D+F S  ++TIGIDF+ KTIEV+GK + LQ+WDTAGQERF+++T  Y+R A G++L+YD T  +SF ++++W+++I++ A   +  +L  NK D+      E ++ VS+E+GE LA +H + F ETSAKS  N+ ++   LAR
Sbjct:    8 LFKLLLIGDSGVGKTCLIIRFAEDNFNSTYISTIGIDFKVKTIEVEGKKVKLQVWDTAGQERFKTITTAYYRGAMGIILVYDITDEKSFENIQNWMKSIKENASAGVSRMLLGNKCDI------EAKRKVSKEMGEKLAKDHGIRFFETSAKSSINVEESFMSLAR 167          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Cavefish
Match: rab8a (RAB8A, member RAS oncogene family [Source:NCBI gene;Acc:103043778])

HSP 1 Score: 149.058 bits (375), Expect = 1.826e-40
Identity = 81/203 (39.90%), Postives = 126/203 (62.07%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNED--LQVMNPIIDLTKEAITSQKNSKASPQCC 2629
            L+K++  GD+ VGK+  + R   D+F S  ++TIGIDF+ +TIE+DGK I LQ+WDTAGQERFR++T  Y+R A G++L+YD T  +SF ++++W+  I + A  ++  ++  NK D+ D      ++ VS+E GE LA E+ + F+ETSAK+  N+      LAR +    D  L+  NP        IT+++  K+S   C
Sbjct:    8 LFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKKIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIKNWIRNIEEHASADVEKMILGNKCDIND------KRQVSKERGEKLALEYGIKFMETSAKANINVENAFLTLARDIKAKMDTKLEGNNPQGSNHGVKITTEQQKKSSFFRC 204          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Cavefish
Match: rab6a (RAB6A, member RAS oncogene family [Source:NCBI gene;Acc:103024949])

HSP 1 Score: 149.058 bits (375), Expect = 2.194e-40
Identity = 82/205 (40.00%), Postives = 120/205 (58.54%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQ--VMNPIIDLTKEAITSQKNSKASPQC 2626
            P R +K+VF G+ SVGK++ ITR   DSF +   ATIGIDF +KT+ ++ + + LQLWDTAGQERFRS+  +Y R +   +++YD T + SF     W++ +R   G+++ I+L  NK+DL D      ++ VS E GE  A E  ++F+ETSAK+G N+ Q    +A  L   E  Q      +ID+  E    Q  S+    C
Sbjct:   10 PLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNVNSFQQTTKWIDDVRTERGSDVIIMLVGNKTDLAD------KRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMESTQDKSREDMIDIKLEKPPEQPVSEGGCSC 208          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Sea Lamprey
Match: rasef (RAS and EF-hand domain containing [Source:ZFIN;Acc:ZDB-GENE-081105-157])

HSP 1 Score: 197.208 bits (500), Expect = 7.486e-53
Identity = 88/170 (51.76%), Postives = 126/170 (74.12%), Query Frame = 2
Query: 2021 ERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVL 2530
            +R+YK+V AGDA+VGKS+F+ RLC + F  D +AT+G+DF+ KT+ VDG+++ LQLWDTAGQERFRS+  +YFR+ADGVLLLYD T  ++F++VRDW++ I  +A   IP++L  NKSD+R+      +K V+   GE LA  +  +F ETSAK G N+++T+  LAR +
Sbjct:  568 DRVYKLVLAGDAAVGKSSFLLRLCRNEFRKDTIATLGVDFQMKTLIVDGEHMTLQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCEKTFLNVRDWIDIIEDSAPKSIPVMLVGNKSDMRNMSPGADQKYVAHTFGEKLAMNYNALFCETSAKDGSNVVETVLHLARYV 737          

HSP 2 Score: 83.5741 bits (205), Expect = 1.320e-16
Identity = 88/363 (24.24%), Postives = 162/363 (44.63%), Query Frame = 2
Query:  362 TLFHTLDPMQRGFINKEDLFSMNCD---LNEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSR--VNSISSWSPP----QEFDKTNNSNYSKNRMCLNNRSSDEGMSLFS---------------CEEKICDFYDTI-SKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELI---YKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETS 1366
            +LF   D    G I+  D FS  C+   L       +F  LD D DG I+  +F  G + I   + +   E    R      +  SP     QE    ++++ S +R  +  RS     S FS                ++ I + Y  I + S  D  + +  ++  +L   +    E + + A   R +      L  +E+E E ++E      +KE ++ AE +  D +   + +  ++ +L  K  +  E   +   +AE+V  L  R   + ++  EN   +  L   QT   +++SEL +LK +M N+    +QE + ++   ++   L++Q+++L+  NRK+QD+ND +RS +E+S
Sbjct:   15 SLFQACDTNHSGDIDY-DEFSDICNELRLQPAQTDALFRDLDADSDGAIDFREFSQGFRCIAASLGQGKGESGSPRDPFPEAAQNSPSCDCGQERQTMDDASSSLSRPLILRRS----FSTFSDFSGRKKNKKLKKCCSQDNIAELYQQIVATSDADLLQSYERVVRDLLGAVERQGAETERVAAILKRSQEQVAERLGEMEDEMEQRLEAHGQKIRKEERARAEHVIGDLKRHYEGEATDL-KLAIKKLKQLENIARSTDSAEDVMELKRR---MGEVTLENQKLKKNLLEAQTNVLVIQSELDSLKMEMTNRTLTFEQEQMTIKELAEERIGLLRQIEVLQATNRKIQDSNDGMRSVVESS 368          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Sea Lamprey
Match: rab8a (RAB8A, member RAS oncogene family [Source:ZFIN;Acc:ZDB-GENE-070424-36])

HSP 1 Score: 145.976 bits (367), Expect = 4.340e-40
Identity = 71/166 (42.77%), Postives = 112/166 (67.47%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELAR 2524
            L+K++  GD+ VGK+  + R   D+F S  ++TIGIDF+ +TI++DGK I LQ+WDTAGQERFR++T  Y+R A G++L+YD T  +SF ++++W+  I + A  ++  ++  NK D+ +      R+ V+RE GE LA E+ + F+ETSAK+  N+ +    LAR
Sbjct:    8 LFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIDLDGKKIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFENIKNWIRNIEEHASADVEKMILGNKCDMNE------RRQVTRERGEKLAIEYGIKFMETSAKASINVEEAFFTLAR 167          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Sea Lamprey
Match: rab30 (RAB30, member RAS oncogene family [Source:ZFIN;Acc:ZDB-GENE-040718-250])

HSP 1 Score: 141.354 bits (355), Expect = 2.311e-38
Identity = 76/169 (44.97%), Postives = 101/169 (59.76%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLA 2533
            L+KIV  G+A VGK+  + R     F     ATIG+DF  KT+EVDG  I LQ+WDTAGQERFRS+T +Y+R A  ++L YD +  E+F  +  W+  + + A   +P VL  NK DL D       + V+RE  E  A  H L ++ETSAK  EN+ +   ELAR LA
Sbjct:    9 LFKIVLIGNAGVGKTCLVRRFTQGLFPPGQGATIGVDFMIKTVEVDGTKIKLQIWDTAGQERFRSITQSYYRSAHALVLTYDVSSEETFACLPAWLREMEQHASAHVPRVLVGNKLDLAD------VRQVTREQAEAFARLHGLRYLETSAKEAENVERLFVELARRLA 171          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Sea Lamprey
Match: rab2a (RAB2A, member RAS oncogene family [Source:ZFIN;Acc:ZDB-GENE-011212-2])

HSP 1 Score: 138.272 bits (347), Expect = 2.453e-37
Identity = 76/199 (38.19%), Postives = 112/199 (56.28%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQ 2623
            L+K +  GD  VGKS  + +     F      TIG++F  + I +DGK + LQ+WDTAGQE FRS+T +Y+R A G LL+YD T  ++F H+  W+E  R+ + + + I+L  NKSDL      E R+ V RE GE  A EH L+F+ETSAK+  N+ +     A+ + +    ++   + D+  EA       K  PQ
Sbjct:    6 LFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQVKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFNHLTTWLEDARQHSNSNMVIMLIGNKSDL------ESRREVKREEGEAFAREHGLVFMETSAKTASNVEEAFINTAKEIYE----KIQEGVFDINNEA----NGIKIGPQ 190          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Sea Lamprey
Match: rab1aa (RAB1A, member RAS oncogene family a [Source:ZFIN;Acc:ZDB-GENE-090312-110])

HSP 1 Score: 136.732 bits (343), Expect = 7.745e-37
Identity = 67/170 (39.41%), Postives = 106/170 (62.35%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAK 2536
            L+K++  GD+ VGKS  + R   D++    ++TIG+DF+ +TIE+DGK I LQ+WDTAGQERFR++T++Y+R A G++++YD T  ESF +V+ W++ I + A   +  +L  NK DL        +K V     +  A   ++ F+ETSAK+  N+ Q    +A  + K
Sbjct:   11 LFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTT------KKVVDYTTAKEFADSLEIPFLETSAKNATNVEQAFMTMAAEIKK 174          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Yeast
Match: SEC4 (Rab family GTPase; essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane [Source:SGD;Acc:S000001889])

HSP 1 Score: 142.124 bits (357), Expect = 8.191e-39
Identity = 69/175 (39.43%), Postives = 111/175 (63.43%), Query Frame = 2
Query: 2021 ERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNED 2545
            + + KI+  GD+ VGKS  + R   D F    + TIGIDF+ KT++++GK + LQLWDTAGQERFR++T  Y+R A G++L+YD T   +F +++ W +T+ + A +E  ++L  NKSD+  R        V+ + GE LA E  + F+E+SAK+ +N+ +    LA+++ +  D
Sbjct:   18 DSIMKILLIGDSGVGKSCLLVRFVEDKFNPSFITTIGIDFKIKTVDINGKKVKLQLWDTAGQERFRTITTAYYRGAMGIILVYDVTDERTFTNIKQWFKTVNEHANDEAQLLLVGNKSDMETR-------VVTADQGEALAKELGIPFIESSAKNDDNVNEIFFTLAKLIQEKID 185          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Yeast
Match: YPT31 (Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication; localizes to the transitional and late Golgi [Source:SGD;Acc:S000000833])

HSP 1 Score: 140.584 bits (353), Expect = 4.042e-38
Identity = 75/209 (35.89%), Postives = 118/209 (56.46%), Query Frame = 2
Query: 1997 SKQLHYMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQ 2623
            S+   Y  + L+KIV  GD+ VGKS  ++R   + F  D  +TIG++F  +T+E+DGK I  Q+WDTAGQER+R++T+ Y+R A G L++YD +   S+ +   W+  +R+ A + + + L  NKSDL    A      V  E  +  A E+QL+F ETSA + EN+ +  EEL   + +    +V    +DL   +     N  ++P 
Sbjct:    3 SEDYGYDYDLLFKIVLIGDSGVGKSNLLSRFTKNEFNMDSKSTIGVEFATRTLEIDGKRIKAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKSSSYENCNHWLSELRENADDNVAVGLIGNKSDLAHLRA------VPTEESKTFAQENQLLFTETSALNSENVDKAFEELINTIYQ----KVSKHQMDLGDSSANGNANGASAPN 201          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Yeast
Match: YPT1 (Rab family GTPase; involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by type II protein geranylgeranyltransferase (Bet2p-Bet4p); binds to unspliced HAC1 mRNA; regulates the unfolded protein response (UPR) by promoting the decay of HAC1 RNA; localizes to the early Golgi, the transitional Golgi and ER membranes, pre-autophagosomal structures, and cytoplasmic vesicles [Source:SGD;Acc:S000001856])

HSP 1 Score: 137.117 bits (344), Expect = 3.329e-37
Identity = 65/178 (36.52%), Postives = 115/178 (64.61%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMN 2560
            L+K++  G++ VGKS  + R   D++ +D ++TIG+DF+ KT+E+DGK + LQ+WDTAGQERFR++T++Y+R + G++++YD T  ESF  V+ W++ I + A + +  +L  NK DL+D      ++ V  ++ +  A  +++ F+ETSA    N+      +AR + ++   Q +N
Sbjct:    8 LFKLLLIGNSGVGKSCLLLRFSDDTYTNDYISTIGVDFKIKTVELDGKTVKLQIWDTAGQERFRTITSSYYRGSHGIIIVYDVTDQESFNGVKMWLQEIDRYATSTVLKLLVGNKCDLKD------KRVVEYDVAKEFADANKMPFLETSALDSTNVEDAFLTMARQIKESMSQQNLN 179          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Yeast
Match: VPS21 (Endosomal Rab family GTPase; required for endocytic transport and sorting of vacuolar hydrolases; required for endosomal localization of the CORVET complex; required with YPT52 for MVB biogenesis and sorting; involved in autophagy and ionic stress tolerance; geranylgeranylation required for membrane association; protein abundance increases in response to DNA replication stress; mammalian Rab5 homolog; VPS21 has a paralog, YPT53, that arose from the whole genome duplication [Source:SGD;Acc:S000005615])

HSP 1 Score: 132.109 bits (331), Expect = 2.449e-35
Identity = 64/155 (41.29%), Postives = 94/155 (60.65%), Query Frame = 2
Query: 2033 KIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENI 2497
            K+V  G+A+VGKS+ + R  ++ F  +   TIG  F  + + ++   +  ++WDTAGQERF S+   Y+R A   L++YD T  +SFI  R WV+ + + A  +I I L  NK D+     E G + V+RE GE LA E  L+F ETSAK+GEN+
Sbjct:    9 KLVLLGEAAVGKSSIVLRFVSNDFAENKEPTIGAAFLTQRVTINEHTVKFEIWDTAGQERFASLAPMYYRNAQAALVVYDVTKPQSFIKARHWVKELHEQASKDIIIALVGNKIDM---LQEGGERKVAREEGEKLAEEKGLLFFETSAKTGENV 160          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Yeast
Match: YPT32 (Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication [Source:SGD;Acc:S000003178])

HSP 1 Score: 132.494 bits (332), Expect = 2.564e-35
Identity = 67/169 (39.64%), Postives = 104/169 (61.54%), Query Frame = 2
Query: 2012 YMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEEL 2518
            Y  + L+KIV  GD+ VGKS  ++R  TD F  +  +TIG++F  +TIEV+ K I  Q+WDTAGQER+R++T+ Y+R A G L++YD +   S+ +   W+  +R+ A + + + L  NKSDL    A      V  +  +N A E+Q++F ETSA + +N+ +   EL
Sbjct:    8 YDYDYLFKIVLIGDSGVGKSNLLSRFTTDEFNIESKSTIGVEFATRTIEVENKKIKAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKSSSYENCNHWLTELRENADDNVAVGLIGNKSDLAHLRA------VPTDEAKNFAMENQMLFTETSALNSDNVDKAFREL 170          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Nematostella
Match: EDO40857 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S613])

HSP 1 Score: 189.119 bits (479), Expect = 1.879e-55
Identity = 91/166 (54.82%), Postives = 117/166 (70.48%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELAR 2524
            ++KIV AGDA+VGKS+FI RLC + F S   +T+G+DF+ KT+ VDGK   LQLWDTAGQERFRS+  +YFRKADGVLLLYD T   SF+ VRDWVE I ++    +PI+L  NK D+R   A E +  ++ E G  LA EH  +F+ETS+K  +NI +   ELAR
Sbjct:    1 MFKIVLAGDAAVGKSSFILRLCRNRFHSALNSTLGVDFQMKTLVVDGKTYALQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCETSFLDVRDWVEAIEESCSKPVPIMLCGNKIDIRQSAAAENKTVITAESGSRLAKEHGALFIETSSKENKNIAEACIELAR 166          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Nematostella
Match: EDO28399 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RL89])

HSP 1 Score: 156.762 bits (395), Expect = 4.306e-44
Identity = 74/165 (44.85%), Postives = 114/165 (69.09%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELA 2521
            ++K+VF GD+ VGKS+F+ R C D +     ATIG+DF+ KT+ V+G+ I +QLWDTAGQERFRS+T  YFRKADG+L+ YD T   SF +++ W+ ++ + A   +  ++  NK+DL +   +E  + V    G+NLA+++  +++ETSAK+G NI + M +LA
Sbjct:    1 VFKVVFIGDSGVGKSSFLHRFCHDQWKPSFTATIGVDFQIKTMNVNGQCIAIQLWDTAGQERFRSITKQYFRKADGILIFYDVTAESSFTNLKSWMVSVEEGAEEGVAKMVLGNKTDLAE---DEESRVVKTIDGKNLANQYDALYMETSAKTGNNIQEAMYKLA 162          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Nematostella
Match: EDO47822 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RL89])

HSP 1 Score: 156.762 bits (395), Expect = 4.306e-44
Identity = 74/165 (44.85%), Postives = 114/165 (69.09%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELA 2521
            ++K+VF GD+ VGKS+F+ R C D +     ATIG+DF+ KT+ V+G+ I +QLWDTAGQERFRS+T  YFRKADG+L+ YD T   SF +++ W+ ++ + A   +  ++  NK+DL +   +E  + V    G+NLA+++  +++ETSAK+G NI + M +LA
Sbjct:    1 VFKVVFIGDSGVGKSSFLHRFCHDQWKPSFTATIGVDFQIKTMNVNGQCIAIQLWDTAGQERFRSITKQYFRKADGILIFYDVTAESSFTNLKSWMVSVEEGAEEGVAKMVLGNKTDLAE---DEESRVVKTIDGKNLANQYDALYMETSAKTGNNIQEAMYKLA 162          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Nematostella
Match: EDO41018 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5I6])

HSP 1 Score: 151.754 bits (382), Expect = 8.603e-42
Identity = 83/203 (40.89%), Postives = 127/203 (62.56%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELAR--VLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCC 2629
            L+K++  GD+ VGK+  + R   D+F S  ++TIGIDF+ +TIE+DGK I LQ+WDTAGQERFR++T  Y+R A G++L+YD T  +SF ++++W+  I + A  ++  ++  NK D+ D      R+ VS+E GE LA E+ + F+ETSAK+  N+ +    LAR   L  ++ L+  NP        IT +K +K S   C
Sbjct:    8 LFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKKIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNDKSFENIKNWIRNIEEHASQDVEKMVLGNKCDMED------RRQVSKERGEQLAVEYGIKFMETSAKASINVEEAFFTLARDIKLKMDKKLENSNP-ASTGAVKITHEKETKRSIFRC 203          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Nematostella
Match: EDO47471 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RMA6])

HSP 1 Score: 149.443 bits (376), Expect = 3.977e-41
Identity = 74/172 (43.02%), Postives = 113/172 (65.70%), Query Frame = 2
Query: 2027 LYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNE 2542
            L+K++  GD+ VGK+  + R   D+F +  ++TIGIDF+ KTIE+ GK I LQ+WDTAGQERF ++T +Y+R A G++L+YD T  ++F ++  W+  I + A  ++  ++  NK D+ D      R+ VSRE GE +A EH + F+ETSAK+  NI Q  + LA+ + K E
Sbjct:    9 LFKLLLIGDSGVGKTCILFRFSDDAFNTTFISTIGIDFKIKTIEMQGKKIKLQIWDTAGQERFHTITTSYYRGAMGIMLVYDITQEKTFDNISKWLRNIEEHANEDVEKMILGNKCDMDD------RRIVSRERGEQIAREHGIRFLETSAKTNINIEQAFQYLAQDILKKE 174          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Medaka
Match: ENSORLT00000027276.1 (calcium release activated channel regulator 2A [Source:NCBI gene;Acc:101161971])

HSP 1 Score: 186.037 bits (471), Expect = 1.127e-48
Identity = 85/176 (48.30%), Postives = 122/176 (69.32%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNED 2545
            P+RL+K+V  G++SVGK+ F+ R C D F     AT+G+D+  KT+ VD   + LQLWDTAGQER+RS+T  +FRKADGV+++YD T  +SF  VR W+ +++++A  EIPI+L  NK+D      +E  + V +++GE LA + QL   E SA SGEN+L++M  LAR+L K ED
Sbjct:  519 PDRLFKVVLVGNSSVGKTCFLRRFCDDCFHPGTCATVGVDYSVKTLTVDNSQVALQLWDTAGQERYRSITKQFFRKADGVVVMYDITAEQSFTAVRQWLSSVKESANEEIPIMLLGNKTD------KEMERKVEKKMGERLAKDCQLSVFECSAYSGENVLESMVHLARILKKQED 688          

HSP 2 Score: 62.3882 bits (150), Expect = 1.836e-9
Identity = 91/419 (21.72%), Postives = 192/419 (45.82%), Query Frame = 2
Query:  365 LFHTLDPMQRGFINKEDLFSMNCDL--NEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSIS----SW--SPPQEFDKTNNSNYS--KNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKI---EKDFRMQLQKKDEEISRLXXXXXXXXXXXXXV-----LSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSL---------RSALETSPSASEIIRRISCTSPPVS--YKAECKLVQCDPAYHKANTCVYTRE--KIDSVTQDSLP 1528
             F   D   +GFIN+ D+  +N +L  +E++L +VF  LD D +G + +++F +G       +H ++++      N        W   P +E +     ++S    R+  NN   D          ++   +  + +  P     F D +  + +   + ++EK+ +     ++   +++ ++HL EE E QI+    +    + E+     +D  +QL  K++E+ +L +K ++ E + +++     ++  EN+++    K    +L+RE      +L   Q +  +L+ +   L ++   ++ R+ +         +D  +L+KQL +LRE+N+ L+D  D +         R+ L+  PS  E+  ++S    P S   +AE +L   + +     + V  R   +I S+ +D LP
Sbjct:   54 FFQMCDLENKGFINRRDMQRLNGELPLSENELENVFDCLDSDSNGFLTLEEFSSGFSAF---LHGQSVDPSTKDGNPCKILPEVWEEGPTREDEYEEEEHFSVLMERLGANNAFEDPA--------EVRSLWAHLRRDEPQLLSNFEDFLARVTSQIMKANQEKKEMECALEKKAAKHDDEIQHLYEEMEQQIKNEQDRIVLQDYERFLSRSRDLELQLSNKEKELEQLFQKQRKLEFQCQELHGKQHVTKVENMKL----KQSNGELERELQYVSQKLMFAQEQLELLQEQSSKLHEEKEMEVYRVTE------GLHRDRASLLKQLDLLREMNKHLRDERDMVHHKPKNSLRRATLKQRPSVDEL--KVSNAEFPRSEDEEAETRLSSKNASSQPCLSAVPGRHLRRIISIEEDHLP 449          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Medaka
Match: ENSORLT00000035474.1 (calcium release activated channel regulator 2A [Source:NCBI gene;Acc:101161971])

HSP 1 Score: 186.037 bits (471), Expect = 1.127e-48
Identity = 85/176 (48.30%), Postives = 122/176 (69.32%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNED 2545
            P+RL+K+V  G++SVGK+ F+ R C D F     AT+G+D+  KT+ VD   + LQLWDTAGQER+RS+T  +FRKADGV+++YD T  +SF  VR W+ +++++A  EIPI+L  NK+D      +E  + V +++GE LA + QL   E SA SGEN+L++M  LAR+L K ED
Sbjct:  519 PDRLFKVVLVGNSSVGKTCFLRRFCDDCFHPGTCATVGVDYSVKTLTVDNSQVALQLWDTAGQERYRSITKQFFRKADGVVVMYDITAEQSFTAVRQWLSSVKESANEEIPIMLLGNKTD------KEMERKVEKKMGERLAKDCQLSVFECSAYSGENVLESMVHLARILKKQED 688          

HSP 2 Score: 62.3882 bits (150), Expect = 1.836e-9
Identity = 91/419 (21.72%), Postives = 192/419 (45.82%), Query Frame = 2
Query:  365 LFHTLDPMQRGFINKEDLFSMNCDL--NEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSIS----SW--SPPQEFDKTNNSNYS--KNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKI---EKDFRMQLQKKDEEISRLXXXXXXXXXXXXXV-----LSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSL---------RSALETSPSASEIIRRISCTSPPVS--YKAECKLVQCDPAYHKANTCVYTRE--KIDSVTQDSLP 1528
             F   D   +GFIN+ D+  +N +L  +E++L +VF  LD D +G + +++F +G       +H ++++      N        W   P +E +     ++S    R+  NN   D          ++   +  + +  P     F D +  + +   + ++EK+ +     ++   +++ ++HL EE E QI+    +    + E+     +D  +QL  K++E+ +L +K ++ E + +++     ++  EN+++    K    +L+RE      +L   Q +  +L+ +   L ++   ++ R+ +         +D  +L+KQL +LRE+N+ L+D  D +         R+ L+  PS  E+  ++S    P S   +AE +L   + +     + V  R   +I S+ +D LP
Sbjct:   54 FFQMCDLENKGFINRRDMQRLNGELPLSENELENVFDCLDSDSNGFLTLEEFSSGFSAF---LHGQSVDPSTKDGNPCKILPEVWEEGPTREDEYEEEEHFSVLMERLGANNAFEDPA--------EVRSLWAHLRRDEPQLLSNFEDFLARVTSQIMKANQEKKEMECALEKKAAKHDDEIQHLYEEMEQQIKNEQDRIVLQDYERFLSRSRDLELQLSNKEKELEQLFQKQRKLEFQCQELHGKQHVTKVENMKL----KQSNGELERELQYVSQKLMFAQEQLELLQEQSSKLHEEKEMEVYRVTE------GLHRDRASLLKQLDLLREMNKHLRDERDMVHHKPKNSLRRATLKQRPSVDEL--KVSNAEFPRSEDEEAETRLSSKNASSQPCLSAVPGRHLRRIISIEEDHLP 449          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Medaka
Match: ENSORLT00000046122.1 (calcium release activated channel regulator 2A [Source:NCBI gene;Acc:101161971])

HSP 1 Score: 186.037 bits (471), Expect = 1.127e-48
Identity = 85/176 (48.30%), Postives = 122/176 (69.32%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNED 2545
            P+RL+K+V  G++SVGK+ F+ R C D F     AT+G+D+  KT+ VD   + LQLWDTAGQER+RS+T  +FRKADGV+++YD T  +SF  VR W+ +++++A  EIPI+L  NK+D      +E  + V +++GE LA + QL   E SA SGEN+L++M  LAR+L K ED
Sbjct:  519 PDRLFKVVLVGNSSVGKTCFLRRFCDDCFHPGTCATVGVDYSVKTLTVDNSQVALQLWDTAGQERYRSITKQFFRKADGVVVMYDITAEQSFTAVRQWLSSVKESANEEIPIMLLGNKTD------KEMERKVEKKMGERLAKDCQLSVFECSAYSGENVLESMVHLARILKKQED 688          

HSP 2 Score: 62.3882 bits (150), Expect = 1.836e-9
Identity = 91/419 (21.72%), Postives = 192/419 (45.82%), Query Frame = 2
Query:  365 LFHTLDPMQRGFINKEDLFSMNCDL--NEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSIS----SW--SPPQEFDKTNNSNYS--KNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKI---EKDFRMQLQKKDEEISRLXXXXXXXXXXXXXV-----LSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSL---------RSALETSPSASEIIRRISCTSPPVS--YKAECKLVQCDPAYHKANTCVYTRE--KIDSVTQDSLP 1528
             F   D   +GFIN+ D+  +N +L  +E++L +VF  LD D +G + +++F +G       +H ++++      N        W   P +E +     ++S    R+  NN   D          ++   +  + +  P     F D +  + +   + ++EK+ +     ++   +++ ++HL EE E QI+    +    + E+     +D  +QL  K++E+ +L +K ++ E + +++     ++  EN+++    K    +L+RE      +L   Q +  +L+ +   L ++   ++ R+ +         +D  +L+KQL +LRE+N+ L+D  D +         R+ L+  PS  E+  ++S    P S   +AE +L   + +     + V  R   +I S+ +D LP
Sbjct:   54 FFQMCDLENKGFINRRDMQRLNGELPLSENELENVFDCLDSDSNGFLTLEEFSSGFSAF---LHGQSVDPSTKDGNPCKILPEVWEEGPTREDEYEEEEHFSVLMERLGANNAFEDPA--------EVRSLWAHLRRDEPQLLSNFEDFLARVTSQIMKANQEKKEMECALEKKAAKHDDEIQHLYEEMEQQIKNEQDRIVLQDYERFLSRSRDLELQLSNKEKELEQLFQKQRKLEFQCQELHGKQHVTKVENMKL----KQSNGELERELQYVSQKLMFAQEQLELLQEQSSKLHEEKEMEVYRVTE------GLHRDRASLLKQLDLLREMNKHLRDERDMVHHKPKNSLRRATLKQRPSVDEL--KVSNAEFPRSEDEEAETRLSSKNASSQPCLSAVPGRHLRRIISIEEDHLP 449          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Medaka
Match: rasef (RAS and EF-hand domain containing [Source:NCBI gene;Acc:101171009])

HSP 1 Score: 184.111 bits (466), Expect = 1.444e-47
Identity = 91/179 (50.84%), Postives = 124/179 (69.27%), Query Frame = 2
Query: 2006 LHYM-PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKN 2539
            L YM  E+ Y+IV AGDA+VGKS+F+ RLC + F  +  AT+G+DF  KT+ VDG+ + LQLWDTAGQERFRS+  +YFR+ADGVLLLYD T  +SF+++R+WV+ I   +  +IPI+L  NK D+R    +E    V    GE LA  +  +F ETSAK G NIL+ +  LAR++ K+
Sbjct:  620 LGYMTSEKAYRIVLAGDAAVGKSSFLLRLCKNEFQLNSSATLGVDFHMKTLIVDGEPVLLQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCEKSFLNIREWVDMIEDVSHEDIPIMLVGNKCDMR----KEEFNCVPISYGEKLAMTYNTLFCETSAKDGSNILEAVLHLARLVKKH 794          

HSP 2 Score: 75.485 bits (184), Expect = 2.320e-13
Identity = 103/442 (23.30%), Postives = 179/442 (40.50%), Query Frame = 2
Query:  353 RATTLFHTLDPMQRGFINKEDLFSMNCDL--NEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSER---KDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALE------TSPSASEIIRR-----ISCTSP---------------PVSYKAEC---KLVQCDPAYHKANTCVYTREKIDSVTQDSLPDEHVLIVGDSGLSTLR 1576
            R  TLF   D    G I + +  ++  +L  + D+   +F +LD D+DG +N+++F  G        H +  E+       +S      E      + Y                      E+    Y  IS + P    Q+  +I +   + K+ + E + L     R +      L  +EEE + +I    +K  + E  + E       +  + E+  L+ K +  +     +    +N+ V  E    K ++ +L  EN   + +L  +QTE   L+SE+ +LK ++ ++    +++   ++ +  +   L  Q++IL+  NRKL D+ND LRSALE      +  S  EI  R     I  +SP               P S +       L  CDP   + ++            +DSLP+  V    DSGLSTL+
Sbjct:  122 RLRTLFRAYDVDNSGRIERNEFLTICSELQVSADEAGRIFERLDVDQDGTVNLEEFIHGF-------HDRYAEEMDVDGGEVSIAWENFELKLGEQAKY------------------IPRHEQAATLYQNISLTEPRLIPQYEKVIMNFTKEIKQQNSEMENLALAIKRAQDQASMQLSEMEEEMDQRIHGAERKTREQEKRRAETALNELRRTYETEVYELQCKVQRMQM----IEEKYKNITVKDESSALKKKINELTLENQRLKQELLKSQTEVAFLQSEMDSLKTELTDQSMNSERDEELMKQFSDERDILESQIEILQTANRKLHDSNDGLRSALEKVTRSGSGGSPGEIRERFRSKSICYSSPHALLERVSERINDLSPFSRQPSSDTLALAICDPGLRRRHSS--------DCEEDSLPEVSV----DSGLSTLK 522          
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Medaka
Match: cracr2ab (ras and EF-hand domain-containing protein homolog [Source:NCBI gene;Acc:101155481])

HSP 1 Score: 172.17 bits (435), Expect = 8.601e-44
Identity = 88/221 (39.82%), Postives = 133/221 (60.18%), Query Frame = 2
Query: 1973 LARPSQNQSKQ-LHYMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCCK 2632
            + + SQ  +K+   + P+RL+K+V  GD+ VGK++ +   C   F      T+GID+  KT+ +D   I +QLWDTAGQER+RS+T  +FRKADGV++++D T  ESF  VR W+  +++AAG  IPI+L  NK D+      E  + VS +  E LA E+ ++F E SA +G N+ +++  LARVL + ED +V +  + LT      +K       CCK
Sbjct:  561 VGKESQQMTKERTLFAPDRLFKVVLVGDSCVGKTSLLRSFCEGRFHPSSTTTVGIDYSVKTLTLDSIQIAMQLWDTAGQERYRSITKQFFRKADGVVVMFDVTLEESFKAVRPWLNNVQEAAGEGIPILLLGNKMDM------EQEREVSFKAAERLAFENNMMFYEVSAYTGMNVTESLTHLARVLIEQED-RVRDTTVTLTAAHPIKKKT------CCK 768          

HSP 2 Score: 65.0846 bits (157), Expect = 2.830e-10
Identity = 82/365 (22.47%), Postives = 162/365 (44.38%), Query Frame = 2
Query:  320 STESLLDDLSMRATT-----LFHTLDPMQRGFINKEDLFSMN--CDLNEDDLLDVFSQLDQDKDGCINVDDFKAG---------LKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIE---KDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSY------EKDWQNLMKQLQILREINRKLQDTND 1339
            STE    DL + A        F T D   +GF+ + D+  ++    L+ ++L DVF  LD D  G + ++ F +          + + + Q        +       S W    E   + + +  +   C+   S    +++F    ++ + +    +  P     F + +  +    KE  +EK+ + +   R+   +++ +RHL EE E+QI+   ++    ++E+++   +D   QL+ K+ E+  L +K K  E +  ++ S  +   V      + + L+  N+     L  T  E  + + +L  L++Q      RLQQE   +  Y      +++ Q+LM+QL +LRE+N+ L+D  D
Sbjct:   14 STERRGSDLGITALLSKTKEFFQTCDVEGKGFLTRLDMRRLHREVPLSAEELEDVFDSLDMDHAGYLTLEAFSSRFSQFLHGRRISVTDDQHQASGPVLKTKEALYQSQW----EAKLSGDEDEEERHFCMLLESLG-AINVFEDPGEVQNLWTQFRRDEPHLLSNFEEFLARVTVQIKEAYQEKKEMESALQRKAATHDSEIRHLYEEMEAQIKTEKERLLLMDSERLQLRSQDLEHQLRSKERELEYLFQKQKRLELQCNELNSEKQESHV------ENLKLKMTNDELSKALECTGQELALAQEQLAMLQEQAR----RLQQEK-EMEMYRVTEGLQREKQSLMRQLDLLREMNKHLKDERD 362          
BLAST of ras and EF-hand domain-containing protein homolog vs. Planmine SMEST
Match: SMESG000076099.1 (SMESG000076099.1)

HSP 1 Score: 1629.38 bits (4218), Expect = 0.000e+0
Identity = 820/824 (99.51%), Postives = 820/824 (99.51%), Query Frame = 2
Query:  161 MKRQSDLITSYELDNNFNSVFDRGPLNDKFDDRRRSVFGSGYFDTFNQMSRASSTESLLDDLSMRATTLFHTLDPMQRGFINKEDLFSMNCDLNEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSPPVSYKAECKLVQCDPAYHKANTCVYTREKIDSVTQDSLPDEHVLIVGDSGLSTLRDVTELESEVEPVNVFSEVCVRQCKFGTSLKSLDKIDDLVNDCQTQSNDKTMVSRPNSLQTXXXXXXXXXXXXXLHQKSDVGELSGNSAQGTSCRQKGIKEKRKYQSKYRDRYPRGDASDSEYPLDKWDSATNLARPSQNQSKQLHYMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCCK 2632
            MKRQSDLITSYELDNNFNSVFDRGPLNDKFDDRRRSVFGSGYFDTFNQMSRASSTESLLDDLSMRATTLFHTLDPMQRGFINKEDLFSMNCDLNEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLKEKFKENEEEFKKVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSPPVSYKAECKLVQCDPAYHKANTCVYTREKIDSVTQDSLPDEH    GDSGLSTLRDVTELESEVEPVNVFSEVCVRQCKFGTSLKSLDKIDDLVNDCQTQSNDKTMVSRPNSLQTDKFRSRRSSKDSSLHQKSDVGELSGNSAQGTSCRQKGIKEKRKYQSKYRDRYPRGDASDSEYPLDKWDSATNLARPSQNQSKQLHYMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCCK
Sbjct:    1 MKRQSDLITSYELDNNFNSVFDRGPLNDKFDDRRRSVFGSGYFDTFNQMSRASSTESLLDDLSMRATTLFHTLDPMQRGFINKEDLFSMNCDLNEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLKEKFKENEEEFKKVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSPPVSYKAECKLVQCDPAYHKANTCVYTREKIDSVTQDSLPDEH----GDSGLSTLRDVTELESEVEPVNVFSEVCVRQCKFGTSLKSLDKIDDLVNDCQTQSNDKTMVSRPNSLQTDKFRSRRSSKDSSLHQKSDVGELSGNSAQGTSCRQKGIKEKRKYQSKYRDRYPRGDASDSEYPLDKWDSATNLARPSQNQSKQLHYMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCCK 820          
BLAST of ras and EF-hand domain-containing protein homolog vs. Planmine SMEST
Match: SMESG000076099.1 (SMESG000076099.1)

HSP 1 Score: 1595.48 bits (4130), Expect = 0.000e+0
Identity = 803/824 (97.45%), Postives = 803/824 (97.45%), Query Frame = 2
Query:  161 MKRQSDLITSYELDNNFNSVFDRGPLNDKFDDRRRSVFGSGYFDTFNQMSRASSTESLLDDLSMRATTLFHTLDPMQRGFINKEDLFSMNCDLNEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLXXXXXXXXXXXXXVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSPPVSYKAECKLVQCDPAYHKANTCVYTREKIDSVTQDSLPDEHVLIVGDSGLSTLRDVTELESEVEPVNVFSEVCVRQCKFGTSLKSLDKIDDLVNDCQTQSNDKTMVSRPNSLQTXXXXXXXXXXXXXLHQKSDVGELSGNSAQGTSCRQKGIKEKRKYQSKYRDRYPRGDASDSEYPLDKWDSATNLARPSQNQSKQLHYMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCCK 2632
            MKRQSDLITSYELDNNFNSVFDRGPLNDKFDDRRRSVFGSGYFDTFNQMSRASSTESLLDDLSMRATTLFHTLDPMQRGFINKEDLFSMNCDLNEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLKEKFKENEEEFKKVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSPPVSYKAECKLVQCDPAYHKANTCVYTREKIDSVTQDSLPDEH    GDSGLSTLRDVTELESEVEPVNVFSEVCVRQCKFGTSLKSLDKIDDLVNDCQTQSNDKTMVSRPNSLQTDKFRSRR                S NSAQGTSCRQKGIKEKRKYQSKYRDRYPRGDASDSEYPLDKWDSATNLARPSQNQSKQLHYMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCCK
Sbjct:    1 MKRQSDLITSYELDNNFNSVFDRGPLNDKFDDRRRSVFGSGYFDTFNQMSRASSTESLLDDLSMRATTLFHTLDPMQRGFINKEDLFSMNCDLNEDDLLDVFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPPQEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDRAEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQIELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLKEKFKENEEEFKKVLSAAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQMNNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALETSPSASEIIRRISCTSPPVSYKAECKLVQCDPAYHKANTCVYTREKIDSVTQDSLPDEH----GDSGLSTLRDVTELESEVEPVNVFSEVCVRQCKFGTSLKSLDKIDDLVNDCQTQSNDKTMVSRPNSLQTDKFRSRR----------------SRNSAQGTSCRQKGIKEKRKYQSKYRDRYPRGDASDSEYPLDKWDSATNLARPSQNQSKQLHYMPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCCK 804          
BLAST of ras and EF-hand domain-containing protein homolog vs. Planmine SMEST
Match: SMESG000028852.1 (SMESG000028852.1)

HSP 1 Score: 178.333 bits (451), Expect = 6.151e-47
Identity = 92/205 (44.88%), Postives = 134/205 (65.37%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEI-PIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCC 2629
            PER++KI+ AGD+SVGK++F+ + C + F     +T+GID+  KT+ VDG+ I LQLWDTAGQE+FRS+ +NYFR+ADG+L++YD     SF++VR W++ IRK+  NE  PI+L +NK D R+  + E  K V ++ GE LA  + L+F E S K   NI   + EL+R L   EDL++    I L ++ I      K + +CC
Sbjct:  379 PERMFKIILAGDSSVGKTSFMLQFCKNIFNPILPSTVGIDYNTKTLFVDGRLIALQLWDTAGQEKFRSIVSNYFRRADGILIMYDVLNPSSFLNVRCWIDIIRKSDINEFTPIMLLANKIDARNSNSTE-HKCVKKKEGELLAKNYDLMFKEISVKEKININTAIIELSRRLKAREDLELSK--IHLNEQDI------KTAKKCC 574          
BLAST of ras and EF-hand domain-containing protein homolog vs. Planmine SMEST
Match: SMESG000028852.1 (SMESG000028852.1)

HSP 1 Score: 177.948 bits (450), Expect = 2.023e-46
Identity = 92/205 (44.88%), Postives = 134/205 (65.37%), Query Frame = 2
Query: 2018 PERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEI-PIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCC 2629
            PER++KI+ AGD+SVGK++F+ + C + F     +T+GID+  KT+ VDG+ I LQLWDTAGQE+FRS+ +NYFR+ADG+L++YD     SF++VR W++ IRK+  NE  PI+L +NK D R+  + E  K V ++ GE LA  + L+F E S K   NI   + EL+R L   EDL++    I L ++ I      K + +CC
Sbjct:  437 PERMFKIILAGDSSVGKTSFMLQFCKNIFNPILPSTVGIDYNTKTLFVDGRLIALQLWDTAGQEKFRSIVSNYFRRADGILIMYDVLNPSSFLNVRCWIDIIRKSDINEFTPIMLLANKIDARNSNSTE-HKCVKKKEGELLAKNYDLMFKEISVKEKININTAIIELSRRLKAREDLELSK--IHLNEQDI------KTAKKCC 632          
BLAST of ras and EF-hand domain-containing protein homolog vs. Planmine SMEST
Match: SMESG000039353.1 (SMESG000039353.1)

HSP 1 Score: 158.303 bits (399), Expect = 9.345e-44
Identity = 81/206 (39.32%), Postives = 123/206 (59.71%), Query Frame = 2
Query: 2015 MPERLYKIVFAGDASVGKSTFITRLCTDSFVSDCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVLLLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRKTVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMNPIIDLTKEAITSQKNSKASPQCCK 2632
            MPE ++K+V  GD++VGKS  + R C  +F  +   TIG+DF  K+IEV+   I LQLWDTAGQER+ S+T  ++RK DGV+L+YD     SFI++R+W++ + K  G+E+ +++  NK DL    A+   K +  +   +L+ E+  +  E SA +GENI ++   LA+VL   +D+ + N        A    K S  S  CCK
Sbjct:    1 MPESVFKVVLVGDSNVGKSAILDRFCNGTFRENYRNTIGVDFMLKSIEVENIIIALQLWDTAGQERYESITKQFYRKTDGVILVYDIMETGSFINIRNWIDNVEKNTGDEVMLMMLGNKLDL----AKSQPKAIPTKNACDLSREYGALMFEVSALTGENINESFATLAKVLKLRQDVDINN--------AFQLTKPSNKSSCCCK 194          
The following BLAST results are available for this feature:
BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RASEF2.400e-5550.24RAS and EF-hand domain containing [Source:HGNC Sym... [more]
CRACR2A4.210e-4846.81calcium release activated channel regulator 2A [So... [more]
RAB6A1.085e-4040.49RAB6A, member RAS oncogene family [Source:HGNC Sym... [more]
RAB6A4.937e-4040.00RAB6A, member RAS oncogene family [Source:HGNC Sym... [more]
RAB6B6.224e-4041.57RAB6B, member RAS oncogene family [Source:HGNC Sym... [more]
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BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rsef-12.803e-4544.44Ras and EF-hand domain-containing protein homolog ... [more]
4R79.21.295e-4140.49pep chromosome:WBcel235:IV:17480396:17482329:-1 ge... [more]
4R79.26.983e-4140.49pep chromosome:WBcel235:IV:17480396:17483332:-1 ge... [more]
rab-81.574e-3944.58RAB family; Rab8 [Source:UniProtKB/TrEMBL;Acc:G5E... [more]
rab-81.574e-3944.58RAB family; Rab8 [Source:UniProtKB/TrEMBL;Acc:G5E... [more]
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BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rab61.744e-3943.27gene:FBgn0015797 transcript:FBtr0080361[more]
Rab61.744e-3943.27gene:FBgn0015797 transcript:FBtr0346437[more]
Rab15.493e-3942.42gene:FBgn0285937 transcript:FBtr0084104[more]
Rab89.527e-3941.44gene:FBgn0262518 transcript:FBtr0308833[more]
Rab89.527e-3941.44gene:FBgn0262518 transcript:FBtr0074921[more]
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BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rasef3.533e-5147.29RAS and EF-hand domain containing [Source:ZFIN;Acc... [more]
CABZ01062083.12.530e-4646.02calcium release activated channel regulator 2Aa [S... [more]
cracr2ab1.254e-4443.72calcium release activated channel regulator 2Ab [S... [more]
cracr2ab1.100e-4343.72calcium release activated channel regulator 2Ab [S... [more]
zgc:1628791.367e-4247.70zgc:162879 [Source:NCBI gene;Acc:572256][more]
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BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
irf61.033e-5252.81interferon regulatory factor 6 [Source:Xenbase;Acc... [more]
irf63.256e-5152.81interferon regulatory factor 6 [Source:Xenbase;Acc... [more]
irf63.729e-5152.81interferon regulatory factor 6 [Source:Xenbase;Acc... [more]
irf64.383e-5153.57interferon regulatory factor 6 [Source:Xenbase;Acc... [more]
erich36.619e-4543.56glutamate rich 3 [Source:Xenbase;Acc:XB-GENE-58807... [more]
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BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rasef2.210e-5248.28RAS and EF hand domain containing [Source:MGI Symb... [more]
Rasef8.008e-5244.08RAS and EF hand domain containing [Source:MGI Symb... [more]
Rasef7.327e-5148.28RAS and EF hand domain containing [Source:MGI Symb... [more]
Cracr2a6.288e-4846.84calcium release activated channel regulator 2A [So... [more]
Rab441.272e-4247.16RAB44, member RAS oncogene family [Source:MGI Symb... [more]
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BLAST of ras and EF-hand domain-containing protein homolog vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8IZ41|RASEF_HUMAN1.152e-5450.24Ras and EF-hand domain-containing protein OS=Homo ... [more]
sp|Q5RI75|RASEF_MOUSE5.602e-5144.08Ras and EF-hand domain-containing protein homolog ... [more]
sp|Q08CX1|RASEF_XENTR1.976e-5052.81Ras and EF-hand domain-containing protein OS=Xenop... [more]
sp|Q22908|RASEF_CAEEL3.565e-4444.44Ras and EF-hand domain-containing protein homolog ... [more]
sp|Q619T5|RASEF_CAEBR1.636e-4344.39Ras and EF-hand domain-containing protein homolog ... [more]
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BLAST of ras and EF-hand domain-containing protein homolog vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2C9LF243.206e-8430.29Uncharacterized protein OS=Biomphalaria glabrata O... [more]
A0A0D9R5K63.891e-7831.36RAS and EF-hand domain containing OS=Chlorocebus s... [more]
A0A096P1V31.551e-7530.99RAS and EF-hand domain containing OS=Papio anubis ... [more]
A0A2K5WTK61.598e-7531.12RAS and EF-hand domain containing OS=Macaca fascic... [more]
G7NGE53.567e-7530.61Ras-related protein Rab-45 OS=Macaca mulatta OX=95... [more]
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BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rasef1.519e-4950.87RAS and EF-hand domain containing [Source:NCBI gen... [more]
zgc:1628791.249e-4247.37ras and EF-hand domain-containing protein-like [So... [more]
rab134.818e-4244.58RAB13, member RAS oncogene family [Source:NCBI gen... [more]
rab8a1.826e-4039.90RAB8A, member RAS oncogene family [Source:NCBI gen... [more]
rab6a2.194e-4040.00RAB6A, member RAS oncogene family [Source:NCBI gen... [more]
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BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rasef7.486e-5351.76RAS and EF-hand domain containing [Source:ZFIN;Acc... [more]
rab8a4.340e-4042.77RAB8A, member RAS oncogene family [Source:ZFIN;Acc... [more]
rab302.311e-3844.97RAB30, member RAS oncogene family [Source:ZFIN;Acc... [more]
rab2a2.453e-3738.19RAB2A, member RAS oncogene family [Source:ZFIN;Acc... [more]
rab1aa7.745e-3739.41RAB1A, member RAS oncogene family a [Source:ZFIN;A... [more]
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BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
SEC48.191e-3939.43Rab family GTPase; essential for vesicle-mediated ... [more]
YPT314.042e-3835.89Rab family GTPase; involved in the exocytic pathwa... [more]
YPT13.329e-3736.52Rab family GTPase; involved in the ER-to-Golgi ste... [more]
VPS212.449e-3541.29Endosomal Rab family GTPase; required for endocyti... [more]
YPT322.564e-3539.64Rab family GTPase involved in the exocytic pathway... [more]
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BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO408571.879e-5554.82Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO283994.306e-4444.85Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO478224.306e-4444.85Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO410188.603e-4240.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO474713.977e-4143.02Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of ras and EF-hand domain-containing protein homolog vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000027276.11.127e-4848.30calcium release activated channel regulator 2A [So... [more]
ENSORLT00000035474.11.127e-4848.30calcium release activated channel regulator 2A [So... [more]
ENSORLT00000046122.11.127e-4848.30calcium release activated channel regulator 2A [So... [more]
rasef1.444e-4750.84RAS and EF-hand domain containing [Source:NCBI gen... [more]
cracr2ab8.601e-4439.82ras and EF-hand domain-containing protein homolog ... [more]
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BLAST of ras and EF-hand domain-containing protein homolog vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000076099.10.000e+099.51SMESG000076099.1[more]
SMESG000076099.10.000e+097.45SMESG000076099.1[more]
SMESG000028852.16.151e-4744.88SMESG000028852.1[more]
SMESG000028852.12.023e-4644.88SMESG000028852.1[more]
SMESG000039353.19.345e-4439.32SMESG000039353.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001660 ID=SMED30001660|Name=ras and EF-hand domain-containing protein homolog|organism=Schmidtea mediterranea sexual|type=transcript|length=3111bp
GTGAGAAGCATTAAACAATCGATTTGGTGCGTATTTCAAGTAATTTAGTC
AAATTTAACATTACAATGAGCTTTCAATTTAATTTTCTATGAGCATTACC
ATTTTTATAGTGCAATAGAAATCTTTTTCGTTTGCCTCTCATCAATATTT
CTTATTTTTAATGAAACGTCAATCTGACCTCATCACTAGCTATGAGTTGG
ACAATAATTTCAATTCGGTTTTCGATCGAGGCCCTTTAAATGACAAATTC
GATGACAGAAGAAGATCGGTTTTTGGGAGTGGATATTTTGACACATTCAA
TCAAATGTCTCGAGCAAGTTCAACGGAATCTTTATTGGATGATCTGTCAA
TGAGAGCCACCACTCTTTTCCACACTTTAGATCCCATGCAAAGAGGATTC
ATAAATAAAGAAGATTTATTTTCGATGAATTGTGATTTGAATGAAGATGA
TCTTTTGGATGTTTTTAGTCAACTAGACCAAGACAAAGACGGTTGCATAA
ATGTCGATGATTTTAAAGCTGGATTGAAACTCATCAATAAACAAATTCAT
AAAAAGAATTTAGAAAAACGACGGAGTCGAGTCAATTCTATTTCGAGTTG
GTCTCCACCTCAGGAGTTCGATAAAACAAACAATTCAAATTATTCAAAAA
ATAGAATGTGTTTAAATAATCGATCTTCAGACGAAGGAATGTCTCTCTTT
TCGTGTGAAGAAAAGATCTGTGATTTCTACGATACTATTAGCAAGTCAAG
TCCAGACCGGGCGGAGCAATTCAATGATATCATTGAAAGCATCCTCAACG
ATACTAAAGAATTATCCAAAGAAAAGCAAGCTCTCCTTGCTAGATTCCAC
AGGGAAAAACTTGCGTATGAAAATAATCTTCGGCATTTAGAAGAGGAATA
CGAATCTCAAATAGAGTTGATTTACAAAAAAGAAACAAAATCTGAGGCCG
AGAAAATAGAAAAAGATTTTCGAATGCAGCTCCAAAAAAAAGATGAAGAA
ATCAGCCGGTTGAAAGAAAAGTTTAAAGAAAATGAAGAAGAGTTCAAGAA
AGTTTTATCGGCTGCAGAGAATGTTCAAGTGTTGTCCGAAAGAAAAGATC
AACTGATGGATCTACAGAGAGAAAATAATAATTTCCAAGTACAACTGTCA
GCAACACAGACAGAATGCACGATGCTCCGCAGTGAGTTGGTCACTTTAAA
GCAACAAATGAACAATAAAATATCTAGGCTTCAACAGGAAAATATTAATT
TGAGGTCATATGAAAAAGACTGGCAAAATTTAATGAAGCAATTACAAATT
TTAAGAGAAATTAATAGGAAGCTTCAAGATACCAATGACAGTCTCAGATC
AGCATTGGAAACATCTCCTTCTGCTAGTGAAATTATTCGTAGAATTAGTT
GTACATCTCCTCCAGTCTCATACAAAGCAGAATGTAAATTAGTTCAGTGT
GATCCGGCTTATCACAAAGCGAACACATGTGTATACACCAGAGAGAAAAT
AGATAGTGTTACCCAGGATTCTCTTCCTGATGAGCATGTTTTAATTGTAG
GAGACAGCGGCTTGTCAACTCTACGAGATGTTACAGAACTGGAATCAGAG
GTAGAACCAGTGAATGTTTTTTCAGAAGTGTGCGTAAGACAATGTAAATT
TGGTACGTCACTCAAGTCTTTGGATAAAATAGATGATTTAGTAAATGATT
GTCAAACACAGTCTAATGATAAGACGATGGTTAGTAGACCCAATAGTCTG
CAGACAGATAAATTCAGAAGTAGACGATCAAGTAAAGATTCGTCTCTTCA
TCAAAAATCTGACGTTGGAGAGTTGTCAGGAAACAGTGCACAAGGAACTT
CGTGTAGACAAAAGGGAATTAAAGAAAAGCGAAAATATCAATCAAAATAT
AGAGATAGATATCCTAGAGGGGATGCAAGTGATTCCGAATATCCACTTGA
TAAATGGGATAGTGCCACTAATTTGGCAAGACCGTCACAGAATCAATCTA
AACAGTTGCATTACATGCCAGAAAGGTTATATAAAATAGTTTTTGCTGGT
GATGCTTCTGTTGGTAAATCTACTTTCATTACGCGTTTATGCACGGATTC
ATTTGTTTCGGACTGCATGGCAACTATTGGCATTGACTTCCGCAACAAAA
CAATAGAAGTTGATGGTAAATACATTGGATTGCAATTGTGGGACACTGCA
GGTCAAGAAAGATTTCGTTCCATGACGGCAAACTATTTTAGAAAGGCTGA
TGGGGTGTTACTGCTTTACGACTGTACGTACATAGAATCATTTATTCATG
TCAGAGATTGGGTGGAAACCATTAGAAAAGCGGCAGGGAATGAAATTCCA
ATAGTTCTTGCATCGAATAAATCTGATCTTAGAGATAGATATGCTGAAGA
AGGACGTAAAACCGTCAGTCGAGAACTCGGAGAGAATTTAGCATCGGAAC
ACCAGTTGATATTTGTGGAAACTAGTGCAAAGAGCGGGGAAAATATTTTA
CAAACAATGGAAGAACTTGCCAGAGTTCTAGCAAAAAATGAGGATTTGCA
AGTTATGAACCCAATCATTGATCTCACTAAAGAAGCAATAACCTCTCAGA
AGAATAGTAAGGCCTCACCTCAGTGTTGCAAATGAGGAGATGGTCCATTC
CTATTAATTCTCATCACCTCATTTTTGAAATTTTTCATTTTTATTATTTC
TCGTAAAAGGATGTTTATTATTTATTCTCGAATAGAGTAACAATTTATTT
TCTGTATTCAGTCTACTGCGATTTATTTCTGAAATTCCTTTTCCGACCTC
ATTAAATATGGCATTGGATATCAATTATTTTTGACCTAACCTGATTGGAA
TATAAAACCTTAAGTCCGGAAGAAAATTATTGAGGAATCCATAATCTAGA
CTTTTAATTTGTGATAAAATGATATCAAATTGTTATACATTCAAAACTTG
AGTATTTTTCTGGTTTTATTTAATTTGAAAAAAATTTCCTCGCGATCCAT
GTAAGAATTATAATAGTCTGATTTTGATTTCTTGATATAAATTTTATTGT
TTTTTTATAATTTATGATTGGTCCAATTGATCTCATGGTTCATTGGATTA
TTTTAGATGAG
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protein sequence of SMED30001660-orf-1

>SMED30001660-orf-1 ID=SMED30001660-orf-1|Name=SMED30001660-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=825bp
MKRQSDLITSYELDNNFNSVFDRGPLNDKFDDRRRSVFGSGYFDTFNQMS
RASSTESLLDDLSMRATTLFHTLDPMQRGFINKEDLFSMNCDLNEDDLLD
VFSQLDQDKDGCINVDDFKAGLKLINKQIHKKNLEKRRSRVNSISSWSPP
QEFDKTNNSNYSKNRMCLNNRSSDEGMSLFSCEEKICDFYDTISKSSPDR
AEQFNDIIESILNDTKELSKEKQALLARFHREKLAYENNLRHLEEEYESQ
IELIYKKETKSEAEKIEKDFRMQLQKKDEEISRLKEKFKENEEEFKKVLS
AAENVQVLSERKDQLMDLQRENNNFQVQLSATQTECTMLRSELVTLKQQM
NNKISRLQQENINLRSYEKDWQNLMKQLQILREINRKLQDTNDSLRSALE
TSPSASEIIRRISCTSPPVSYKAECKLVQCDPAYHKANTCVYTREKIDSV
TQDSLPDEHVLIVGDSGLSTLRDVTELESEVEPVNVFSEVCVRQCKFGTS
LKSLDKIDDLVNDCQTQSNDKTMVSRPNSLQTDKFRSRRSSKDSSLHQKS
DVGELSGNSAQGTSCRQKGIKEKRKYQSKYRDRYPRGDASDSEYPLDKWD
SATNLARPSQNQSKQLHYMPERLYKIVFAGDASVGKSTFITRLCTDSFVS
DCMATIGIDFRNKTIEVDGKYIGLQLWDTAGQERFRSMTANYFRKADGVL
LLYDCTYIESFIHVRDWVETIRKAAGNEIPIVLASNKSDLRDRYAEEGRK
TVSRELGENLASEHQLIFVETSAKSGENILQTMEELARVLAKNEDLQVMN
PIIDLTKEAITSQKNSKASPQCCK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003924GTPase activity
GO:0005509calcium ion binding
GO:0005525GTP binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR005225Small_GTP-bd_dom
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR001806Small_GTPase
IPR027417P-loop_NTPase
IPR018247EF_Hand_1_Ca_BS
Vocabulary: biological process
TermDefinition
GO:0007264small GTPase mediated signal transduction
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 308..335
NoneNo IPR availableCOILSCoilCoilcoord: 208..253
NoneNo IPR availableCOILSCoilCoilcoord: 267..305
NoneNo IPR availableCOILSCoilCoilcoord: 340..401
NoneNo IPR availablePRINTSPR00449RASTRNSFRMNGcoord: 647..663
score: 31.97
coord: 768..790
score: 34.13
coord: 727..740
score: 43.64
coord: 665..687
score: 54.09
coord: 624..645
score: 37.12
NoneNo IPR availableSMARTSM00176ran_sub_2coord: 629..821
e-value: 2.8E-5
score: 25.8
NoneNo IPR availableSMARTSM00174rho_sub_3coord: 626..794
e-value: 1.9E-8
score: -10.3
NoneNo IPR availableSMARTSM00175rab_sub_5coord: 624..793
e-value: 1.6E-63
score: 227.1
NoneNo IPR availableSMARTSM00173ras_sub_4coord: 621..793
e-value: 5.6E-23
score: 92.3
NoneNo IPR availableSMARTSM00177arf_sub_2coord: 609..794
e-value: 0.0074
score: -2.7
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 612..821
e-value: 3.2E-58
score: 198.3
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 61..156
e-value: 9.4E-8
score: 33.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 567..595
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 550..566
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 518..595
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 532..549
NoneNo IPR availablePANTHERPTHR22621UNCHARACTERIZEDcoord: 65..484
NoneNo IPR availablePANTHERPTHR22621:SF6RAS AND EF-HAND DOMAIN-CONTAINING PROTEINcoord: 611..824
NoneNo IPR availablePANTHERPTHR22621UNCHARACTERIZEDcoord: 611..824
NoneNo IPR availablePANTHERPTHR22621:SF6RAS AND EF-HAND DOMAIN-CONTAINING PROTEINcoord: 65..484
NoneNo IPR availablePROSITEPS51419RABcoord: 619..824
score: 30.201
NoneNo IPR availableCDDcd00154Rabcoord: 624..788
e-value: 7.03607E-67
score: 217.324
IPR002048EF-hand domainSMARTSM00054efh_1coord: 97..125
e-value: 0.0047
score: 26.1
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 93..128
score: 13.425
IPR005225Small GTP-binding protein domainTIGRFAMTIGR00231TIGR00231coord: 623..784
e-value: 1.6E-28
score: 97.5
IPR001806Small GTPasePFAMPF00071Rascoord: 625..790
e-value: 2.3E-48
score: 163.9
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 106..118
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 621..809
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 67..134