eIF-2-alpha kinase GCN2

Overview
NameeIF-2-alpha kinase GCN2
Smed IDSMED30001649
Length (bp)1774
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of eIF-2-alpha kinase GCN2 (SMED30001649) t-SNE clustered cells

Violin plots show distribution of expression levels for eIF-2-alpha kinase GCN2 (SMED30001649) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of eIF-2-alpha kinase GCN2 (SMED30001649) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for eIF-2-alpha kinase GCN2 (SMED30001649) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30001649

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 13

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pigment cellSMED30001649SMESG000015477.1 SmedASXL_008508SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
parenchymal cellSMED30001649SMESG000078676.1 SMESG000078140.1 SMESG000059486.1 dd_Smed_v4_10190_0_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30001649SMESG000015477.1 dd_Smed_v4_4357_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30001649SMESG000015477.1 dd_Smed_v4_4357_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30001649SMESG000015477.1 dd_Smed_v4_4357_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001649SMESG000018751.1 SMESG000018455.1 SMESG000016745.1 SMESG000016709.1 Contig21346uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001649SMESG000018751.1 SMESG000018455.1 SMESG000016745.1 SMESG000016709.1 Contig21346newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001649SMESG000081199.1 SMESG000078676.1 SMESG000078140.1 SMESG000076970.1 SMESG000072047.1 SMESG000071195.1 SMESG000066830.1 SMESG000066452.1 SMESG000066025.1 SMESG000064686.1 SMESG000052186.1 SMESG000050985.1 SMESG000049426.1 SMESG000048739.1 SMESG000042719.1 SMESG000042112.1 SMESG000038917.1 SMESG000036780.1 SMESG000033800.1 SMESG000028851.1 SMESG000025323.1 SMESG000023339.1 SMESG000022364.1 SMESG000018751.1 SMESG000018455.1 SMESG000016863.1 SMESG000016709.1 SMESG000010388.1 SMESG000009317.1 SMESG000006929.1 SMESG000005645.1 SMESG000005237.1 SMESG000005163.1 SMESG000003711.1 SMESG000002725.1 Contig28831uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001649SMESG000081199.1 SMESG000078676.1 SMESG000078140.1 SMESG000076970.1 SMESG000072047.1 SMESG000071195.1 SMESG000066830.1 SMESG000066452.1 SMESG000066025.1 SMESG000064686.1 SMESG000052186.1 SMESG000050985.1 SMESG000049426.1 SMESG000048739.1 SMESG000042719.1 SMESG000042112.1 SMESG000038917.1 SMESG000036780.1 SMESG000033800.1 SMESG000028851.1 SMESG000025323.1 SMESG000023339.1 SMESG000022364.1 SMESG000018751.1 SMESG000018455.1 SMESG000016863.1 SMESG000016709.1 SMESG000010388.1 SMESG000009317.1 SMESG000006929.1 SMESG000005645.1 SMESG000005237.1 SMESG000005163.1 SMESG000003711.1 SMESG000002725.1 Contig28831newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001649SMESG000052154.1 SMESG000050985.1 SMESG000049041.1 SMESG000048739.1 SMESG000027422.1 SMESG000020005.1 Contig11059uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30001649SMESG000052154.1 SMESG000050985.1 SMESG000049041.1 SMESG000048739.1 SMESG000027422.1 SMESG000020005.1 Contig11059newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30001649 dd_Smed_v4_11648_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30001649 dd_Smed_v4_11648_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of eIF-2-alpha kinase GCN2 vs. Planmine SMEST
Match: SMESG000002725.1 (SMESG000002725.1)

HSP 1 Score: 598.971 bits (1543), Expect = 0.000e+0
Identity = 302/341 (88.56%), Postives = 311/341 (91.20%), Query Frame = 1 
Query:  589 MNSDIGSDHELFCDNVSNNSLWENAEFSNANKLPFIKKLDKDDSYEQILIPGSSFPKMKINNRESNLKTSNKIESCFIKDFNTLFSLDNKIISDKVSSSLIEEKLQFNSPICNSEYWLKLNVGRTPFTEKFQNSPLCKPSYNSRTNTLFGSKIFLDDIQDCFGSNDSNAIDSKNSVLEIRKGNLLKYINYSQVKPYENEQCKNVDCKLQMKLMFLHFFYLNERSITLRVMDKHYYRKPDLEGDLNDRYISVYNSFHVFPKFCDKVKLQKFVGVKYFRAKHRFNSHWYCIKISSYNNDGIQTLNKSSRLLQNEIMMLSRLSHENIIKYFSSWIEYQITSRSN 1611
            MNSDIGSD+ELF  N SNNSL ENAEFSNANK PFIKKLDKDDSYEQILI GSSFPKMK+NN ESNLKTSNKIE+CFIKDFNTLFSLDNKIISDKVSSSLIEEKLQFNSPICNSEYWLKLNV RTPFTEKFQNSPLCKPSYNSRTN LFG KI LDDIQDC GSNDSNAIDSK SVLEIRKG+LLKYINYSQVK YEN+QCKNVD KLQMKLMFLHFFYLNERSI LRVMDKH YRKPDL+G  NDRYIS YNSFHV P FCD VKL   VGVKYFRAKHRF+SHWYCIKISSYNND IQTL+KSSRL+QNEIM LSRLSHENIIKYFSSWIEYQ TS SN
Sbjct:    1 MNSDIGSDYELFYHNDSNNSLCENAEFSNANKFPFIKKLDKDDSYEQILISGSSFPKMKVNNCESNLKTSNKIENCFIKDFNTLFSLDNKIISDKVSSSLIEEKLQFNSPICNSEYWLKLNVVRTPFTEKFQNSPLCKPSYNSRTNILFGYKILLDDIQDCSGSNDSNAIDSKKSVLEIRKGDLLKYINYSQVKSYENKQCKNVDSKLQMKLMFLHFFYLNERSIILRVMDKHEYRKPDLDGYFNDRYISAYNSFHVSPTFCDMVKLHSLVGVKYFRAKHRFDSHWYCIKISSYNNDDIQTLSKSSRLVQNEIMKLSRLSHENIIKYFSSWIEYQTTSGSN 341
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BLAST of eIF-2-alpha kinase GCN2 vs. Planmine SMEST
Match: SMESG000052154.1 (SMESG000052154.1)

HSP 1 Score: 569.311 bits (1466), Expect = 0.000e+0
Identity = 284/335 (84.78%), Postives = 297/335 (88.66%), Query Frame = 1 
Query:  589 MNSDIGSDHELFCDNVSNNSLWENAEFSNANKLPFIKKLDKDDSYEQILIPGSSFPKMKINNRESNLKTSNKIESCFIKDFNTLFSLDNKIISDKVSSSLIEEKLQFNSPICNSEYWLKLNVGRTPFTEKFQNSPLCKPSYNSRTNTLFGSKIFLDDIQDCFGSNDSNAIDSKNSVLEIRKGNLLKYINYSQVKPYENEQCKNVDCKLQMKLMFLHFFYLNERSITLRVMDKHYYRKPDLEGDLNDRYISVYNSFHVFPKFCDKVKLQKFVGVKYFRAKHRFNSHWYCIKISSYNNDGIQTLNKSSRLLQNEIMMLSRLSHENIIKYFSSWIEYQ 1593
            MNSDIGSDHELF DN SNNSLWEN+EFSNAN  PFIKKLDKDDSYEQI IPG SF K+K+NN E NL T+NKIE CFIKDFNT FSLDNK+ISDKVSSSLIEEKL+FN+PICNSEYWLKLNVGRTPF EKFQNSPLCKPSYN RTNTLFGSK  LDDIQDC GSNDSNAIDSK SVLEIRKGN LKYINYSQVK YENEQ K+VD KL MKLMFLH FYLNE S+ LRV+DKH YRK DLEGDLNDRYISV NS HV P FCD VKL   VG+KYFRAKHRF+SHWYCIKISSYNND IQTL+KSSRLLQNEIMMLS LSHENIIKYFSSWIEYQ
Sbjct:    1 MNSDIGSDHELFSDNDSNNSLWENSEFSNANIFPFIKKLDKDDSYEQIFIPGLSFYKIKVNNCEGNLNTANKIERCFIKDFNTSFSLDNKVISDKVSSSLIEEKLEFNTPICNSEYWLKLNVGRTPFQEKFQNSPLCKPSYNFRTNTLFGSKTLLDDIQDCSGSNDSNAIDSKKSVLEIRKGNFLKYINYSQVKSYENEQYKDVDSKLPMKLMFLHCFYLNESSLILRVVDKHDYRKSDLEGDLNDRYISVDNSIHVSPIFCDMVKLHSLVGIKYFRAKHRFDSHWYCIKISSYNNDEIQTLSKSSRLLQNEIMMLSSLSHENIIKYFSSWIEYQ 335
[Close]
BLAST of eIF-2-alpha kinase GCN2 vs. Planmine SMEST
Match: SMESG000078140.1 (SMESG000078140.1)

HSP 1 Score: 542.347 bits (1396), Expect = 0.000e+0
Identity = 276/319 (86.52%), Postives = 283/319 (88.71%), Query Frame = 1 
Query:  622 FCDNVSNNSLWENAEFSNANKLPFIKKLDKDDSYEQILIPGSSFPKMKINNRESNLKTSNKIESCFIKDFNTLFSLDNKIISDKVSSSLIEEKLQFNSPICNSEYWLKLNVGRTPFTEKFQNSPLCKPSYNSRTNTLFGSKIFLDDIQDCFGSNDSNAIDSKNSVLEIRKGNLLKYINYSQVKPYENEQCKNVDCKLQMKLMFLHFFYLNERSITLRVMDKHYYRKPDLEGDLNDRYISVYNSFHVFPKFCDKVKLQKFVGVKYFRAKHRFNSHWYCIKISSYNNDGIQTLNKSSRLLQNEIMMLSRLSHENIIKYFSS 1578
            FCDN SNNSLWENAEFSNA K PFIKKLDKDDSY+QILIPGSSFPK KINN ESNL TSNKIESCFIKDFNTLFSLDNKIISDK S SLIEEKLQFNSPIC SEYWLKLNVGRTPFT KFQNSPLCKPSYNSRT TLFGSK  LDDIQD   +NDSNAIDSK SVLEIRKGNLLKYINYSQVK YENEQCKN D KLQMK MFL+FFYLN+RSI LRVMDKH  RKPDLEGDLNDRY S YNSFHV   FCD V+L   VGVKYF AKHRF+SHWY IKISSYNND IQTL+KSSRLLQNEIMMLSRLSHENIIKYFSS
Sbjct:    4 FCDNDSNNSLWENAEFSNAKKFPFIKKLDKDDSYKQILIPGSSFPKKKINNYESNLNTSNKIESCFIKDFNTLFSLDNKIISDKASFSLIEEKLQFNSPICKSEYWLKLNVGRTPFTGKFQNSPLCKPSYNSRTYTLFGSKFLLDDIQDFSVTNDSNAIDSKKSVLEIRKGNLLKYINYSQVKSYENEQCKNFDSKLQMKRMFLNFFYLNKRSIILRVMDKHENRKPDLEGDLNDRYSSAYNSFHVSLTFCDMVELHSLVGVKYFSAKHRFDSHWYYIKISSYNNDEIQTLSKSSRLLQNEIMMLSRLSHENIIKYFSS 322
[Close]
BLAST of eIF-2-alpha kinase GCN2 vs. Planmine SMEST
Match: SMESG000066452.1 (SMESG000066452.1)

HSP 1 Score: 520.776 bits (1340), Expect = 0.000e+0
Identity = 268/341 (78.59%), Postives = 280/341 (82.11%), Query Frame = 1 
Query:  589 MNSDIGSDHELFCDNVSNNSLWENAEFSNANKLPFIKKLDKDDSYEQILIPGSSFPKMKINNRESNLKTSNKIESCFIKDFNTLFSLDNKIISDKVSSSLIEEKLQFNSPICNSEYWLKLNVGRTPFTEKFQNSPLCKPSYNSRTNTLFGSKIFLDDIQDCFGSNDSNAIDSKNSVLEIRKGNLLKYINYSQVKPYENEQCKNVDCKLQMKLMFLHFFYLNERSITLRVMDKHYYRKPDLEGDLNDRYISVYNSFHVFPKFCDKVKLQKFVGVKYFRAKHRFNSHWYCIKISSYNNDGIQTLNKSSRLLQNEIMMLSRLSHENIIKYFSSWIEYQITSRSN 1611
            MNSDI SDHELFC N SNNSL ENA+ SNANK PFIKKLDKDDSYEQI IPGSSFPKMK+NN E+N KTSNK                         S+L      FNSPICNSEYWLKLNVGRTPFTEKFQNSPLCKPSYNSRTNTLFGSKI LDDIQD  GSN+SNA DSK S+LEIRKGNLLKYINYSQVK YENEQCKNVD KLQMKLMFLHFFYLN+RSI LRV+DKH YRKPDL+GDLNDRYIS YNSFHV P FC  VKL   VGVKYFRAKHRF+ HWYCIKISSYNND IQTL+KSSRLLQNEIMMLSRLSHENIIKY SSWI+YQ TSRSN
Sbjct:    1 MNSDIRSDHELFCANDSNNSLMENAKLSNANKFPFIKKLDKDDSYEQIFIPGSSFPKMKVNNCENNSKTSNK-------------------------SAL------FNSPICNSEYWLKLNVGRTPFTEKFQNSPLCKPSYNSRTNTLFGSKILLDDIQDYSGSNESNANDSKKSMLEIRKGNLLKYINYSQVKRYENEQCKNVDSKLQMKLMFLHFFYLNDRSIILRVVDKHEYRKPDLKGDLNDRYISAYNSFHVSPTFCHMVKLHSLVGVKYFRAKHRFDCHWYCIKISSYNNDEIQTLSKSSRLLQNEIMMLSRLSHENIIKYLSSWIKYQTTSRSN 310
[Close]
BLAST of eIF-2-alpha kinase GCN2 vs. Planmine SMEST
Match: SMESG000010388.1 (SMESG000010388.1)

HSP 1 Score: 502.286 bits (1292), Expect = 6.034e-177
Identity = 247/288 (85.76%), Postives = 259/288 (89.93%), Query Frame = 1 
Query:  589 MNSDIGSDHELFCDNVSNNSLWENAEFSNANKLPFIKKLDKDDSYEQILIPGSSFPKMKINNRESNLKTSNKIESCFIKDFNTLFSLDNKIISDKVSSSLIEEKLQFNSPICNSEYWLKLNVGRTPFTEKFQNSPLCKPSYNSRTNTLFGSKIFLDDIQDCFGSNDSNAIDSKNSVLEIRKGNLLKYINYSQVKPYENEQCKNVDCKLQMKLMFLHFFYLNERSITLRVMDKHYYRKPDLEGDLNDRYISVYNSFHVFPKFCDKVKLQKFVGVKYFRAKHRFNSHWYC 1452
            MNSDIGSD+ELFCDN SNNSLW+NA+ S  NK PFIKKLDKDDSYEQILIP SSFPKMK+NN ESNL TSNKIESC IKDFNTLFSLDNKIIS+KVSSSLIEEKL+FN+ ICNSEYWLKLNVGRTPFTEKFQ +PLCKPS+NSRTNTLFGSKI LDDIQDC GSNDSN+IDSK SVLEIRKGNLLKYINYSQVK YENEQCKNV+ KLQMKLMFLHFF LNERSI LRVMDKH Y KPDLEGDLND YIS YNSFHV P FCD VKL   VGVKY RAKHRF+SHWYC
Sbjct:    1 MNSDIGSDYELFCDNDSNNSLWKNAKLSKENKFPFIKKLDKDDSYEQILIPSSSFPKMKVNNCESNLNTSNKIESCIIKDFNTLFSLDNKIISNKVSSSLIEEKLEFNTSICNSEYWLKLNVGRTPFTEKFQKTPLCKPSFNSRTNTLFGSKILLDDIQDCSGSNDSNSIDSKKSVLEIRKGNLLKYINYSQVKSYENEQCKNVNSKLQMKLMFLHFFNLNERSIILRVMDKHEYMKPDLEGDLNDHYISAYNSFHVSPTFCDMVKLHSLVGVKYVRAKHRFDSHWYC 288
The following BLAST results are available for this feature:
BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of eIF-2-alpha kinase GCN2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of eIF-2-alpha kinase GCN2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of eIF-2-alpha kinase GCN2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000002725.10.000e+088.56SMESG000002725.1[more]
SMESG000052154.10.000e+084.78SMESG000052154.1[more]
SMESG000078140.10.000e+086.52SMESG000078140.1[more]
SMESG000066452.10.000e+078.59SMESG000066452.1[more]
SMESG000010388.16.034e-17785.76SMESG000010388.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001649 ID=SMED30001649|Name=eIF-2-alpha kinase GCN2|organism=Schmidtea mediterranea sexual|type=transcript|length=1774bp
TAACTAAATCATTTGCATGTCAGATTCCACTTGAATAAAATTATTATAAA
TTTATTTCTTAACACATGATTTGATAATTATTACATGTCGTAACCAGCAG
CGTTTTATTTATAATGAAATTTATCTCAAAAAATATAAAATAAAATGATC
AGTTCACGTGATGTCTCTAAGAAAAATGGAAGGTTGACGATTAAAGATGC
TTCGATTTCAAAGAAAAATTACGTAGAACTTCAATTTATGGGATCGTAAA
TCATTATTCAATTATTAATTAAACATAATAATTAAACATAATGATTAAAC
ATTTTAATTAAACTAAATTAAATCATGATTATGTAGATAAAAATAAATTG
TAGAGGACCTTGCAATTGAAATTCTCTAAGATTAAATAAAAGAAAAAGTT
TTGTGATATTATTTAATTTACTTAGATTAATATGTGTGTTTTAATAAAAT
GTGTAAATTGTGTGTTTTAAAAAATATAGAATTTATTTGTTGATTAATTG
ACTTTGTTAAATTTAAAATTTCGTTCAAGAGCCTTTGGATTTCATTAAAA
ACTTAATAAAAAGTTATTCAATAACCATATACTGAAATATGAATAGCGAT
ATTGGATCTGATCATGAACTATTTTGCGATAATGTTTCAAATAATAGTTT
ATGGGAAAATGCTGAGTTTTCTAATGCAAACAAATTACCCTTTATTAAGA
AATTAGATAAAGACGATTCTTATGAACAAATTCTCATTCCTGGTTCGAGT
TTTCCTAAAATGAAAATAAACAATCGTGAAAGCAATTTGAAAACATCCAA
TAAAATTGAAAGTTGCTTTATTAAAGATTTCAATACTTTATTCTCTCTGG
ACAATAAAATAATTTCTGATAAAGTTTCATCTTCATTGATTGAAGAAAAG
CTCCAATTCAATTCACCGATATGCAACAGCGAGTACTGGCTCAAATTGAA
TGTTGGGCGGACACCTTTTACAGAAAAATTCCAAAATTCTCCTCTTTGCA
AGCCGTCCTATAATTCTCGAACTAACACTTTGTTCGGCTCAAAAATCTTC
TTGGATGACATTCAAGATTGTTTTGGAAGCAATGATTCTAATGCCATTGA
TAGTAAAAACTCAGTGTTAGAAATTCGAAAAGGCAACTTGTTGAAATATA
TTAATTACAGTCAGGTAAAACCCTATGAAAATGAACAATGTAAAAATGTC
GACTGTAAATTACAAATGAAACTGATGTTTCTGCATTTCTTTTATTTAAA
TGAAAGAAGTATAACTTTGAGAGTCATGGATAAGCATTATTATAGGAAGC
CGGATTTGGAAGGAGATTTAAATGATCGCTATATTTCGGTTTATAATTCT
TTCCATGTTTTTCCAAAATTTTGTGACAAGGTGAAACTACAAAAGTTTGT
TGGTGTCAAATATTTTCGTGCTAAGCACAGATTTAATTCCCATTGGTATT
GCATTAAGATCAGCTCGTATAATAATGATGGGATACAAACCCTAAATAAA
TCAAGTAGACTTCTGCAAAATGAAATCATGATGCTTTCACGTCTAAGTCA
TGAAAATATTATTAAATATTTTTCATCGTGGATCGAATACCAAATTACTT
CCCGATCAAACAACATTAAATATTCATAATAATGAGGTATGACATTGTCT
ATTAATGAAAAGGTTGATGGCAATGAAAGTGATTATGTACTAATTTAATA
CTTGCTCATGATTATAATTGAATAAATTAGGTAAGTCTGAAGTTTATTGC
GATTTAGGCTTTTCGAATATGTAC
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protein sequence of SMED30001649-orf-1

>SMED30001649-orf-1 ID=SMED30001649-orf-1|Name=SMED30001649-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=347bp
MNSDIGSDHELFCDNVSNNSLWENAEFSNANKLPFIKKLDKDDSYEQILI
PGSSFPKMKINNRESNLKTSNKIESCFIKDFNTLFSLDNKIISDKVSSSL
IEEKLQFNSPICNSEYWLKLNVGRTPFTEKFQNSPLCKPSYNSRTNTLFG
SKIFLDDIQDCFGSNDSNAIDSKNSVLEIRKGNLLKYINYSQVKPYENEQ
CKNVDCKLQMKLMFLHFFYLNERSITLRVMDKHYYRKPDLEGDLNDRYIS
VYNSFHVFPKFCDKVKLQKFVGVKYFRAKHRFNSHWYCIKISSYNNDGIQ
TLNKSSRLLQNEIMMLSRLSHENIIKYFSSWIEYQITSRSNNIKYS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0002075pigment cell
Vocabulary: INTERPRO
TermDefinition
IPR011009Kinase-like_dom_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.30.200.20coord: 59..132
e-value: 9.3E-12
score: 46.8
IPR011009Protein kinase-like domain superfamilySUPERFAMILYSSF56112Protein kinase-like (PK-like)coord: 57..125