eIF-2-alpha kinase GCN2
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30001649 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 13
Homology
BLAST of eIF-2-alpha kinase GCN2 vs. Planmine SMEST
Match: SMESG000002725.1 (SMESG000002725.1) HSP 1 Score: 598.971 bits (1543), Expect = 0.000e+0 Identity = 302/341 (88.56%), Postives = 311/341 (91.20%), Query Frame = 1 Query: 589 MNSDIGSDHELFCDNVSNNSLWENAEFSNANKLPFIKKLDKDDSYEQILIPGSSFPKMKINNRESNLKTSNKIESCFIKDFNTLFSLDNKIISDKVSSSLIEEKLQFNSPICNSEYWLKLNVGRTPFTEKFQNSPLCKPSYNSRTNTLFGSKIFLDDIQDCFGSNDSNAIDSKNSVLEIRKGNLLKYINYSQVKPYENEQCKNVDCKLQMKLMFLHFFYLNERSITLRVMDKHYYRKPDLEGDLNDRYISVYNSFHVFPKFCDKVKLQKFVGVKYFRAKHRFNSHWYCIKISSYNNDGIQTLNKSSRLLQNEIMMLSRLSHENIIKYFSSWIEYQITSRSN 1611 MNSDIGSD+ELF N SNNSL ENAEFSNANK PFIKKLDKDDSYEQILI GSSFPKMK+NN ESNLKTSNKIE+CFIKDFNTLFSLDNKIISDKVSSSLIEEKLQFNSPICNSEYWLKLNV RTPFTEKFQNSPLCKPSYNSRTN LFG KI LDDIQDC GSNDSNAIDSK SVLEIRKG+LLKYINYSQVK YEN+QCKNVD KLQMKLMFLHFFYLNERSI LRVMDKH YRKPDL+G NDRYIS YNSFHV P FCD VKL VGVKYFRAKHRF+SHWYCIKISSYNND IQTL+KSSRL+QNEIM LSRLSHENIIKYFSSWIEYQ TS SN Sbjct: 1 MNSDIGSDYELFYHNDSNNSLCENAEFSNANKFPFIKKLDKDDSYEQILISGSSFPKMKVNNCESNLKTSNKIENCFIKDFNTLFSLDNKIISDKVSSSLIEEKLQFNSPICNSEYWLKLNVVRTPFTEKFQNSPLCKPSYNSRTNILFGYKILLDDIQDCSGSNDSNAIDSKKSVLEIRKGDLLKYINYSQVKSYENKQCKNVDSKLQMKLMFLHFFYLNERSIILRVMDKHEYRKPDLDGYFNDRYISAYNSFHVSPTFCDMVKLHSLVGVKYFRAKHRFDSHWYCIKISSYNNDDIQTLSKSSRLVQNEIMKLSRLSHENIIKYFSSWIEYQTTSGSN 341
BLAST of eIF-2-alpha kinase GCN2 vs. Planmine SMEST
Match: SMESG000052154.1 (SMESG000052154.1) HSP 1 Score: 569.311 bits (1466), Expect = 0.000e+0 Identity = 284/335 (84.78%), Postives = 297/335 (88.66%), Query Frame = 1 Query: 589 MNSDIGSDHELFCDNVSNNSLWENAEFSNANKLPFIKKLDKDDSYEQILIPGSSFPKMKINNRESNLKTSNKIESCFIKDFNTLFSLDNKIISDKVSSSLIEEKLQFNSPICNSEYWLKLNVGRTPFTEKFQNSPLCKPSYNSRTNTLFGSKIFLDDIQDCFGSNDSNAIDSKNSVLEIRKGNLLKYINYSQVKPYENEQCKNVDCKLQMKLMFLHFFYLNERSITLRVMDKHYYRKPDLEGDLNDRYISVYNSFHVFPKFCDKVKLQKFVGVKYFRAKHRFNSHWYCIKISSYNNDGIQTLNKSSRLLQNEIMMLSRLSHENIIKYFSSWIEYQ 1593 MNSDIGSDHELF DN SNNSLWEN+EFSNAN PFIKKLDKDDSYEQI IPG SF K+K+NN E NL T+NKIE CFIKDFNT FSLDNK+ISDKVSSSLIEEKL+FN+PICNSEYWLKLNVGRTPF EKFQNSPLCKPSYN RTNTLFGSK LDDIQDC GSNDSNAIDSK SVLEIRKGN LKYINYSQVK YENEQ K+VD KL MKLMFLH FYLNE S+ LRV+DKH YRK DLEGDLNDRYISV NS HV P FCD VKL VG+KYFRAKHRF+SHWYCIKISSYNND IQTL+KSSRLLQNEIMMLS LSHENIIKYFSSWIEYQ Sbjct: 1 MNSDIGSDHELFSDNDSNNSLWENSEFSNANIFPFIKKLDKDDSYEQIFIPGLSFYKIKVNNCEGNLNTANKIERCFIKDFNTSFSLDNKVISDKVSSSLIEEKLEFNTPICNSEYWLKLNVGRTPFQEKFQNSPLCKPSYNFRTNTLFGSKTLLDDIQDCSGSNDSNAIDSKKSVLEIRKGNFLKYINYSQVKSYENEQYKDVDSKLPMKLMFLHCFYLNESSLILRVVDKHDYRKSDLEGDLNDRYISVDNSIHVSPIFCDMVKLHSLVGIKYFRAKHRFDSHWYCIKISSYNNDEIQTLSKSSRLLQNEIMMLSSLSHENIIKYFSSWIEYQ 335
BLAST of eIF-2-alpha kinase GCN2 vs. Planmine SMEST
Match: SMESG000078140.1 (SMESG000078140.1) HSP 1 Score: 542.347 bits (1396), Expect = 0.000e+0 Identity = 276/319 (86.52%), Postives = 283/319 (88.71%), Query Frame = 1 Query: 622 FCDNVSNNSLWENAEFSNANKLPFIKKLDKDDSYEQILIPGSSFPKMKINNRESNLKTSNKIESCFIKDFNTLFSLDNKIISDKVSSSLIEEKLQFNSPICNSEYWLKLNVGRTPFTEKFQNSPLCKPSYNSRTNTLFGSKIFLDDIQDCFGSNDSNAIDSKNSVLEIRKGNLLKYINYSQVKPYENEQCKNVDCKLQMKLMFLHFFYLNERSITLRVMDKHYYRKPDLEGDLNDRYISVYNSFHVFPKFCDKVKLQKFVGVKYFRAKHRFNSHWYCIKISSYNNDGIQTLNKSSRLLQNEIMMLSRLSHENIIKYFSS 1578 FCDN SNNSLWENAEFSNA K PFIKKLDKDDSY+QILIPGSSFPK KINN ESNL TSNKIESCFIKDFNTLFSLDNKIISDK S SLIEEKLQFNSPIC SEYWLKLNVGRTPFT KFQNSPLCKPSYNSRT TLFGSK LDDIQD +NDSNAIDSK SVLEIRKGNLLKYINYSQVK YENEQCKN D KLQMK MFL+FFYLN+RSI LRVMDKH RKPDLEGDLNDRY S YNSFHV FCD V+L VGVKYF AKHRF+SHWY IKISSYNND IQTL+KSSRLLQNEIMMLSRLSHENIIKYFSS Sbjct: 4 FCDNDSNNSLWENAEFSNAKKFPFIKKLDKDDSYKQILIPGSSFPKKKINNYESNLNTSNKIESCFIKDFNTLFSLDNKIISDKASFSLIEEKLQFNSPICKSEYWLKLNVGRTPFTGKFQNSPLCKPSYNSRTYTLFGSKFLLDDIQDFSVTNDSNAIDSKKSVLEIRKGNLLKYINYSQVKSYENEQCKNFDSKLQMKRMFLNFFYLNKRSIILRVMDKHENRKPDLEGDLNDRYSSAYNSFHVSLTFCDMVELHSLVGVKYFSAKHRFDSHWYYIKISSYNNDEIQTLSKSSRLLQNEIMMLSRLSHENIIKYFSS 322
BLAST of eIF-2-alpha kinase GCN2 vs. Planmine SMEST
Match: SMESG000066452.1 (SMESG000066452.1) HSP 1 Score: 520.776 bits (1340), Expect = 0.000e+0 Identity = 268/341 (78.59%), Postives = 280/341 (82.11%), Query Frame = 1 Query: 589 MNSDIGSDHELFCDNVSNNSLWENAEFSNANKLPFIKKLDKDDSYEQILIPGSSFPKMKINNRESNLKTSNKIESCFIKDFNTLFSLDNKIISDKVSSSLIEEKLQFNSPICNSEYWLKLNVGRTPFTEKFQNSPLCKPSYNSRTNTLFGSKIFLDDIQDCFGSNDSNAIDSKNSVLEIRKGNLLKYINYSQVKPYENEQCKNVDCKLQMKLMFLHFFYLNERSITLRVMDKHYYRKPDLEGDLNDRYISVYNSFHVFPKFCDKVKLQKFVGVKYFRAKHRFNSHWYCIKISSYNNDGIQTLNKSSRLLQNEIMMLSRLSHENIIKYFSSWIEYQITSRSN 1611 MNSDI SDHELFC N SNNSL ENA+ SNANK PFIKKLDKDDSYEQI IPGSSFPKMK+NN E+N KTSNK S+L FNSPICNSEYWLKLNVGRTPFTEKFQNSPLCKPSYNSRTNTLFGSKI LDDIQD GSN+SNA DSK S+LEIRKGNLLKYINYSQVK YENEQCKNVD KLQMKLMFLHFFYLN+RSI LRV+DKH YRKPDL+GDLNDRYIS YNSFHV P FC VKL VGVKYFRAKHRF+ HWYCIKISSYNND IQTL+KSSRLLQNEIMMLSRLSHENIIKY SSWI+YQ TSRSN Sbjct: 1 MNSDIRSDHELFCANDSNNSLMENAKLSNANKFPFIKKLDKDDSYEQIFIPGSSFPKMKVNNCENNSKTSNK-------------------------SAL------FNSPICNSEYWLKLNVGRTPFTEKFQNSPLCKPSYNSRTNTLFGSKILLDDIQDYSGSNESNANDSKKSMLEIRKGNLLKYINYSQVKRYENEQCKNVDSKLQMKLMFLHFFYLNDRSIILRVVDKHEYRKPDLKGDLNDRYISAYNSFHVSPTFCHMVKLHSLVGVKYFRAKHRFDCHWYCIKISSYNNDEIQTLSKSSRLLQNEIMMLSRLSHENIIKYLSSWIKYQTTSRSN 310
BLAST of eIF-2-alpha kinase GCN2 vs. Planmine SMEST
Match: SMESG000010388.1 (SMESG000010388.1) HSP 1 Score: 502.286 bits (1292), Expect = 6.034e-177 Identity = 247/288 (85.76%), Postives = 259/288 (89.93%), Query Frame = 1 Query: 589 MNSDIGSDHELFCDNVSNNSLWENAEFSNANKLPFIKKLDKDDSYEQILIPGSSFPKMKINNRESNLKTSNKIESCFIKDFNTLFSLDNKIISDKVSSSLIEEKLQFNSPICNSEYWLKLNVGRTPFTEKFQNSPLCKPSYNSRTNTLFGSKIFLDDIQDCFGSNDSNAIDSKNSVLEIRKGNLLKYINYSQVKPYENEQCKNVDCKLQMKLMFLHFFYLNERSITLRVMDKHYYRKPDLEGDLNDRYISVYNSFHVFPKFCDKVKLQKFVGVKYFRAKHRFNSHWYC 1452 MNSDIGSD+ELFCDN SNNSLW+NA+ S NK PFIKKLDKDDSYEQILIP SSFPKMK+NN ESNL TSNKIESC IKDFNTLFSLDNKIIS+KVSSSLIEEKL+FN+ ICNSEYWLKLNVGRTPFTEKFQ +PLCKPS+NSRTNTLFGSKI LDDIQDC GSNDSN+IDSK SVLEIRKGNLLKYINYSQVK YENEQCKNV+ KLQMKLMFLHFF LNERSI LRVMDKH Y KPDLEGDLND YIS YNSFHV P FCD VKL VGVKY RAKHRF+SHWYC Sbjct: 1 MNSDIGSDYELFCDNDSNNSLWKNAKLSKENKFPFIKKLDKDDSYEQILIPSSSFPKMKVNNCESNLNTSNKIESCIIKDFNTLFSLDNKIISNKVSSSLIEEKLEFNTSICNSEYWLKLNVGRTPFTEKFQKTPLCKPSFNSRTNTLFGSKILLDDIQDCSGSNDSNSIDSKKSVLEIRKGNLLKYINYSQVKSYENEQCKNVNSKLQMKLMFLHFFNLNERSIILRVMDKHEYMKPDLEGDLNDHYISAYNSFHVSPTFCDMVKLHSLVGVKYVRAKHRFDSHWYC 288 The following BLAST results are available for this feature:
BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of eIF-2-alpha kinase GCN2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of eIF-2-alpha kinase GCN2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of eIF-2-alpha kinase GCN2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of eIF-2-alpha kinase GCN2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30001649 ID=SMED30001649|Name=eIF-2-alpha kinase GCN2|organism=Schmidtea mediterranea sexual|type=transcript|length=1774bpback to top protein sequence of SMED30001649-orf-1 >SMED30001649-orf-1 ID=SMED30001649-orf-1|Name=SMED30001649-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=347bp MNSDIGSDHELFCDNVSNNSLWENAEFSNANKLPFIKKLDKDDSYEQILIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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