SMED30001625

Overview
NameSMED30001625
Smed IDSMED30001625
Length (bp)3200
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30001625

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30001625h1SMcG0006986 SmedASXL_003749SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30001625 vs. Planmine SMEST
Match: SMESG000044311.1 (SMESG000044311.1)

HSP 1 Score: 902.894 bits (2332), Expect = 0.000e+0
Identity = 458/480 (95.42%), Postives = 459/480 (95.62%), Query Frame = 2 
Query:   32 LIVYEGFHERIIDSDSYGVYIDQDIQNFNIENKFIFKVVPKVFNSSLKIIADNFTNVFYNRTTGIVICTFRYDRRDINWDKRIVVEGLNNKSIIIVKFKDKEKGIKIHCPTQYIVRSAVQQAFCTPYLAEEKETDMFRHRKYPITYTYFYGHKVQPAIYTDDIVEKNKFEYKIRVEMTIRRVADLISKTTEVKLILFGIPFSPFTNYNWSFPHRINIKKEKLVFILSSSEIYLDHFVIFTVNGLRLKFQNITITKQYSDFPSLPEYFDIKVTGYILIKLNSMPEKYQILNGVISFLLYETTDYFFPITHVFSTVALNCPTKSKINSKRIQIKMQFPKLYSSFVISNKTDLIVSNRWKEGSHAIVKCLALMAPSLTTVFKDPNHRILQXXXXXXXXXXXANCHFVPRSSKIILPDIILFACVCHASAKIVHIITFPVCKIVSHVGCQLSVDCQTFDKEILNIQISVGYDSSATFFNKTYKK 1471
            + V  GFHERIIDSDSYGVYIDQDIQNFNIENKFIFKVVPKVFNSSLKIIADNFTNVFYNRTTGIVICTFRYDRRDINWDKRIVVEGLNNKSIIIVKFKDKEKGIKIHCPTQYIVRSAVQQAFCTPYLAEEKETDMFRHRKYPITYTYFYGHKVQPAIYTDDIVEKNKFEYKIRVEMTIRRVADLISKTTEVKLILFGIPFSPFTNYNWSFPHRINIKKEKLVFILSSSEIYLDHFVIFTVNGLRLKFQNITITKQYSDFPSLPEYFDIKVTGYILIKLNSMPEKYQILNGVISFLLYETTDYFFPITHVFSTVALNCPTKSKINSKRIQIKMQFPKLYSSFVISNKTDLIVSNRWKEGSHAIVKCLALMAPSLTTVFKDPNHRILQVKDVKIIKKDIANCHFVPRSSKIILPDIILFACVCHASAKI                  LSVDCQTFDKEILNIQISVGYDSSATFFNKTYKK
Sbjct:  139 VFVIPGFHERIIDSDSYGVYIDQDIQNFNIENKFIFKVVPKVFNSSLKIIADNFTNVFYNRTTGIVICTFRYDRRDINWDKRIVVEGLNNKSIIIVKFKDKEKGIKIHCPTQYIVRSAVQQAFCTPYLAEEKETDMFRHRKYPITYTYFYGHKVQPAIYTDDIVEKNKFEYKIRVEMTIRRVADLISKTTEVKLILFGIPFSPFTNYNWSFPHRINIKKEKLVFILSSSEIYLDHFVIFTVNGLRLKFQNITITKQYSDFPSLPEYFDIKVTGYILIKLNSMPEKYQILNGVISFLLYETTDYFFPITHVFSTVALNCPTKSKINSKRIQIKMQFPKLYSSFVISNKTDLIVSNRWKEGSHAIVKCLALMAPSLTTVFKDPNHRILQVKDVKIIKKDIANCHFVPRSSKIILPDIILFACVCHASAKI------------------LSVDCQTFDKEILNIQISVGYDSSATFFNKTYKK 600

HSP 2 Score: 571.622 bits (1472), Expect = 0.000e+0
Identity = 273/279 (97.85%), Postives = 275/279 (98.57%), Query Frame = 1 
Query: 1465 QKTFPTVVFYHPRYPRLNTIITCMTDTENSILQFRWVCNHTDKYLEFMLVDFFKTQGQFMVFLNTAPVVNYSCICIVKIRNFKTGSAITIYQNFTMQLRETSLMFCNMKSIIRSSYSKSEIFLYIIVTTLLFMGFPLLAYIITEYQSINSVHVDHLYRLGIADNAETEEQNKIPSFCFSKHNLGEKQFDLFIHLVERCTKNEEKFRRLKASLDLLLEKNKMFQLAKLKYCFNHHEINEHGEQSELHPERILTKKSHMTFEEKKCSSTHFLERIFVRKYM 2301
            + +FPTVVFYHPRYPRLNTIITCMTDTENSILQFRWVCNHTDKYLEFMLVDFFKTQGQFMVFLNTAPVVNYSCICIVKIRNFKTGSAITIYQNFTMQLRETSLMFCNMKSIIRSSYSKSEIFLYIIVTTLLFMGFPLLAYIITEYQSINSVHVDHLYRLGIADNAETEEQNKIPSFCFSKHNLGEKQFDLFIHLVERCTKNEEKFRRLKASLDLLLEKNKMFQLAKLKYCFNHHEINEHGEQSELHPERILTKKSHMTFEEKKCSSTHFLERIF  K M
Sbjct:  600 KASFPTVVFYHPRYPRLNTIITCMTDTENSILQFRWVCNHTDKYLEFMLVDFFKTQGQFMVFLNTAPVVNYSCICIVKIRNFKTGSAITIYQNFTMQLRETSLMFCNMKSIIRSSYSKSEIFLYIIVTTLLFMGFPLLAYIITEYQSINSVHVDHLYRLGIADNAETEEQNKIPSFCFSKHNLGEKQFDLFIHLVERCTKNEEKFRRLKASLDLLLEKNKMFQLAKLKYCFNHHEINEHGEQSELHPERILTKKSHMTFEEKKCSSTHFLERIFQMKSM 878

HSP 3 Score: 420.239 bits (1079), Expect = 0.000e+0
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 2 
Query: 2459 SDFSKPLVDMYFNSPVKPRRSVTQIMNFKTPRLLSRTNFXXXXXXXXXXXKNEKKPIIRNLETEHSKSSEKKPIINNAEDDHSKSTQFLEKILQMKAMKDHSKSEYNLAYRENSAQSRPRIGHVDSDISRRLDLYFNPPRKYRRSATQVLDIKTSNLKSKTNFSIEPLDSSNFLKNEENLVVSNSDDEYLKSNFQYGTLFYPASNPELFPTKTRNENL 3112
            SDFSKPLVDMYFNSPVKPRRSVTQIMNFKTPRLLSRTNFSAESLAALSLLKNEKKPIIRNLETEHSKSSEKKPIINNAEDDHSKSTQFLEKILQMKAMKDHSKSEYNLAYRENSAQSRPRIGHVDSDISRRLDLYFNPPRKYRRSATQVLDIKTSNLKSKTNFSIEPLDSSNFLKNEENLVVSNSDDEYLKSNFQYGTLFYPASNPELFPTKTRNENL
Sbjct:  917 SDFSKPLVDMYFNSPVKPRRSVTQIMNFKTPRLLSRTNFSAESLAALSLLKNEKKPIIRNLETEHSKSSEKKPIINNAEDDHSKSTQFLEKILQMKAMKDHSKSEYNLAYRENSAQSRPRIGHVDSDISRRLDLYFNPPRKYRRSATQVLDIKTSNLKSKTNFSIEPLDSSNFLKNEENLVVSNSDDEYLKSNFQYGTLFYPASNPELFPTKTRNENL 1134

HSP 4 Score: 86.6557 bits (213), Expect = 0.000e+0
Identity = 41/41 (100.00%), Postives = 41/41 (100.00%), Query Frame = 3 
Query: 2334 QMKSMSDHSKNEYHYAYRENRLKSNVEEIFNQVERNPRISN 2456
            QMKSMSDHSKNEYHYAYRENRLKSNVEEIFNQVERNPRISN
Sbjct:  874 QMKSMSDHSKNEYHYAYRENRLKSNVEEIFNQVERNPRISN 914

HSP 5 Score: 108.612 bits (270), Expect = 1.158e-23
Identity = 65/149 (43.62%), Postives = 90/149 (60.40%), Query Frame = 2 
Query: 2648 EHSKSSEKKP--IINNA-----EDDHSKSTQFLEKILQMKAMKDHSKSEYNLAYRENSAQS-----------RPRIGHVDSDISRRL-DLYFNPPRKYRRSATQVLDIKTSNLKSKTNFSIEPLDSSNFLKNEENLVVSNSDDEYLKSN 3037
            EH + SE  P  I+        E+    ST FLE+I QMK+M DHSK+EY+ AYREN  +S            PRI +++SD S+ L D+YFN P K RRS TQ+++ KT  L S+TNFS E L + + LKNE+  ++ N + E+ KS+
Sbjct:  837 EHGEQSELHPERILTKKSHMTFEEKKCSSTHFLERIFQMKSMSDHSKNEYHYAYRENRLKSNVEEIFNQVERNPRISNIESDFSKPLVDMYFNSPVKPRRSVTQIMNFKTPRLLSRTNFSAESLAALSLLKNEKKPIIRNLETEHSKSS 985
[Close]
BLAST of SMED30001625 vs. Planmine SMEST
Match: SMESG000044311.1 (SMESG000044311.1)

HSP 1 Score: 881.322 bits (2276), Expect = 0.000e+0
Identity = 449/480 (93.54%), Postives = 450/480 (93.75%), Query Frame = 2 
Query:   32 LIVYEGFHERIIDSDSYGVYIDQDIQNFNIENKFIFKVVPKVFNSSLKIIADNFTNVFYNRTTGIVICTFRYDRRDINWDKRIVVEGLNNKSIIIVKFKDKEKGIKIHCPTQYIVRSAVQQAFCTPYLAEEKETDMFRHRKYPITYTYFYGHKVQPAIYTDDIVEKNKFEYKIRVEMTIRRVADLISKTTEVKLILFGIPFSPFTNYNWSFPHRINIKKEKLVFILSSSEIYLDHFVIFTVNGLRLKFQNITITKQYSDFPSLPEYFDIKVTGYILIKLNSMPEKYQILNGVISFLLYETTDYFFPITHVFSTVALNCPTKSKINSKRIQIKMQFPKLYSSFVISNKTDLIVSNRWKEGSHAIVKCLALMAPSLTTVFKDPNHRILQXXXXXXXXXXXANCHFVPRSSKIILPDIILFACVCHASAKIVHIITFPVCKIVSHVGCQLSVDCQTFDKEILNIQISVGYDSSATFFNKTYKK 1471
            + V  GFHERIIDSDSYGVYIDQDIQNFNIENKFIFKVVPKVFNSSLKIIADNFTNVFYNRTTGIVICTFRYDRRDINWDKRIVVEGLNNKSIIIVKFKDKEKGIKIHCPTQYIVRSAVQQAFCTPYLAEEKETDMFRHRKYPITYTYFYGHKVQPAIYTDDIVEKNKFEYKIRVEMTIRRVADLISKTTEVKLILFGIPFSPFTNYNWSFPHRINIKKEKLVFILSSSEIYLDHFVIFTVNGLRLKFQNITITKQYSDFPSLPEYFDIKVTGYILIKLNSMPEKYQILNGVISFLLYETTDYFFPITHVFSTVALNCPTKSKINSKRIQIKMQFPKLYSSFVISNKTDLIVSNRWKEGSHAIVKCLALMAPSLTTVFKDPNHRILQVKDVKIIKKDIANCHFVPRSSKIILPDIILFACVCHASAKIVHIITFP                           ISVGYDSSATFFNKTYKK
Sbjct:  139 VFVIPGFHERIIDSDSYGVYIDQDIQNFNIENKFIFKVVPKVFNSSLKIIADNFTNVFYNRTTGIVICTFRYDRRDINWDKRIVVEGLNNKSIIIVKFKDKEKGIKIHCPTQYIVRSAVQQAFCTPYLAEEKETDMFRHRKYPITYTYFYGHKVQPAIYTDDIVEKNKFEYKIRVEMTIRRVADLISKTTEVKLILFGIPFSPFTNYNWSFPHRINIKKEKLVFILSSSEIYLDHFVIFTVNGLRLKFQNITITKQYSDFPSLPEYFDIKVTGYILIKLNSMPEKYQILNGVISFLLYETTDYFFPITHVFSTVALNCPTKSKINSKRIQIKMQFPKLYSSFVISNKTDLIVSNRWKEGSHAIVKCLALMAPSLTTVFKDPNHRILQVKDVKIIKKDIANCHFVPRSSKIILPDIILFACVCHASAKIVHIITFP---------------------------ISVGYDSSATFFNKTYKK 591

HSP 2 Score: 571.622 bits (1472), Expect = 0.000e+0
Identity = 273/279 (97.85%), Postives = 275/279 (98.57%), Query Frame = 1 
Query: 1465 QKTFPTVVFYHPRYPRLNTIITCMTDTENSILQFRWVCNHTDKYLEFMLVDFFKTQGQFMVFLNTAPVVNYSCICIVKIRNFKTGSAITIYQNFTMQLRETSLMFCNMKSIIRSSYSKSEIFLYIIVTTLLFMGFPLLAYIITEYQSINSVHVDHLYRLGIADNAETEEQNKIPSFCFSKHNLGEKQFDLFIHLVERCTKNEEKFRRLKASLDLLLEKNKMFQLAKLKYCFNHHEINEHGEQSELHPERILTKKSHMTFEEKKCSSTHFLERIFVRKYM 2301
            + +FPTVVFYHPRYPRLNTIITCMTDTENSILQFRWVCNHTDKYLEFMLVDFFKTQGQFMVFLNTAPVVNYSCICIVKIRNFKTGSAITIYQNFTMQLRETSLMFCNMKSIIRSSYSKSEIFLYIIVTTLLFMGFPLLAYIITEYQSINSVHVDHLYRLGIADNAETEEQNKIPSFCFSKHNLGEKQFDLFIHLVERCTKNEEKFRRLKASLDLLLEKNKMFQLAKLKYCFNHHEINEHGEQSELHPERILTKKSHMTFEEKKCSSTHFLERIF  K M
Sbjct:  591 KASFPTVVFYHPRYPRLNTIITCMTDTENSILQFRWVCNHTDKYLEFMLVDFFKTQGQFMVFLNTAPVVNYSCICIVKIRNFKTGSAITIYQNFTMQLRETSLMFCNMKSIIRSSYSKSEIFLYIIVTTLLFMGFPLLAYIITEYQSINSVHVDHLYRLGIADNAETEEQNKIPSFCFSKHNLGEKQFDLFIHLVERCTKNEEKFRRLKASLDLLLEKNKMFQLAKLKYCFNHHEINEHGEQSELHPERILTKKSHMTFEEKKCSSTHFLERIFQMKSM 869

HSP 3 Score: 420.239 bits (1079), Expect = 0.000e+0
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 2 
Query: 2459 SDFSKPLVDMYFNSPVKPRRSVTQIMNFKTPRLLSRTNFXXXXXXXXXXXKNEKKPIIRNLETEHSKSSEKKPIINNAEDDHSKSTQFLEKILQMKAMKDHSKSEYNLAYRENSAQSRPRIGHVDSDISRRLDLYFNPPRKYRRSATQVLDIKTSNLKSKTNFSIEPLDSSNFLKNEENLVVSNSDDEYLKSNFQYGTLFYPASNPELFPTKTRNENL 3112
            SDFSKPLVDMYFNSPVKPRRSVTQIMNFKTPRLLSRTNFSAESLAALSLLKNEKKPIIRNLETEHSKSSEKKPIINNAEDDHSKSTQFLEKILQMKAMKDHSKSEYNLAYRENSAQSRPRIGHVDSDISRRLDLYFNPPRKYRRSATQVLDIKTSNLKSKTNFSIEPLDSSNFLKNEENLVVSNSDDEYLKSNFQYGTLFYPASNPELFPTKTRNENL
Sbjct:  908 SDFSKPLVDMYFNSPVKPRRSVTQIMNFKTPRLLSRTNFSAESLAALSLLKNEKKPIIRNLETEHSKSSEKKPIINNAEDDHSKSTQFLEKILQMKAMKDHSKSEYNLAYRENSAQSRPRIGHVDSDISRRLDLYFNPPRKYRRSATQVLDIKTSNLKSKTNFSIEPLDSSNFLKNEENLVVSNSDDEYLKSNFQYGTLFYPASNPELFPTKTRNENL 1125

HSP 4 Score: 86.6557 bits (213), Expect = 0.000e+0
Identity = 41/41 (100.00%), Postives = 41/41 (100.00%), Query Frame = 3 
Query: 2334 QMKSMSDHSKNEYHYAYRENRLKSNVEEIFNQVERNPRISN 2456
            QMKSMSDHSKNEYHYAYRENRLKSNVEEIFNQVERNPRISN
Sbjct:  865 QMKSMSDHSKNEYHYAYRENRLKSNVEEIFNQVERNPRISN 905

HSP 5 Score: 106.301 bits (264), Expect = 6.848e-23
Identity = 65/149 (43.62%), Postives = 90/149 (60.40%), Query Frame = 2 
Query: 2648 EHSKSSEKKP--IIN-----NAEDDHSKSTQFLEKILQMKAMKDHSKSEYNLAYRENSAQS-----------RPRIGHVDSDISRRL-DLYFNPPRKYRRSATQVLDIKTSNLKSKTNFSIEPLDSSNFLKNEENLVVSNSDDEYLKSN 3037
            EH + SE  P  I+        E+    ST FLE+I QMK+M DHSK+EY+ AYREN  +S            PRI +++SD S+ L D+YFN P K RRS TQ+++ KT  L S+TNFS E L + + LKNE+  ++ N + E+ KS+
Sbjct:  828 EHGEQSELHPERILTKKSHMTFEEKKCSSTHFLERIFQMKSMSDHSKNEYHYAYRENRLKSNVEEIFNQVERNPRISNIESDFSKPLVDMYFNSPVKPRRSVTQIMNFKTPRLLSRTNFSAESLAALSLLKNEKKPIIRNLETEHSKSS 976
[Close]
BLAST of SMED30001625 vs. Planmine SMEST
Match: SMESG000044311.1 (SMESG000044311.1)

HSP 1 Score: 789.645 bits (2038), Expect = 0.000e+0
Identity = 400/419 (95.47%), Postives = 401/419 (95.70%), Query Frame = 2 
Query:  215 TTGIVICTFRYDRRDINWDKRIVVEGLNNKSIIIVKFKDKEKGIKIHCPTQYIVRSAVQQAFCTPYLAEEKETDMFRHRKYPITYTYFYGHKVQPAIYTDDIVEKNKFEYKIRVEMTIRRVADLISKTTEVKLILFGIPFSPFTNYNWSFPHRINIKKEKLVFILSSSEIYLDHFVIFTVNGLRLKFQNITITKQYSDFPSLPEYFDIKVTGYILIKLNSMPEKYQILNGVISFLLYETTDYFFPITHVFSTVALNCPTKSKINSKRIQIKMQFPKLYSSFVISNKTDLIVSNRWKEGSHAIVKCLALMAPSLTTVFKDPNHRILQXXXXXXXXXXXANCHFVPRSSKIILPDIILFACVCHASAKIVHIITFPVCKIVSHVGCQLSVDCQTFDKEILNIQISVGYDSSATFFNKTYKK 1471
            TTGIVICTFRYDRRDINWDKRIVVEGLNNKSIIIVKFKDKEKGIKIHCPTQYIVRSAVQQAFCTPYLAEEKETDMFRHRKYPITYTYFYGHKVQPAIYTDDIVEKNKFEYKIRVEMTIRRVADLISKTTEVKLILFGIPFSPFTNYNWSFPHRINIKKEKLVFILSSSEIYLDHFVIFTVNGLRLKFQNITITKQYSDFPSLPEYFDIKVTGYILIKLNSMPEKYQILNGVISFLLYETTDYFFPITHVFSTVALNCPTKSKINSKRIQIKMQFPKLYSSFVISNKTDLIVSNRWKEGSHAIVKCLALMAPSLTTVFKDPNHRILQVKDVKIIKKDIANCHFVPRSSKIILPDIILFACV                  +SHVGCQLSVDCQTFDKEILNIQISVGYDSSATFFNKTYKK
Sbjct:  140 TTGIVICTFRYDRRDINWDKRIVVEGLNNKSIIIVKFKDKEKGIKIHCPTQYIVRSAVQQAFCTPYLAEEKETDMFRHRKYPITYTYFYGHKVQPAIYTDDIVEKNKFEYKIRVEMTIRRVADLISKTTEVKLILFGIPFSPFTNYNWSFPHRINIKKEKLVFILSSSEIYLDHFVIFTVNGLRLKFQNITITKQYSDFPSLPEYFDIKVTGYILIKLNSMPEKYQILNGVISFLLYETTDYFFPITHVFSTVALNCPTKSKINSKRIQIKMQFPKLYSSFVISNKTDLIVSNRWKEGSHAIVKCLALMAPSLTTVFKDPNHRILQVKDVKIIKKDIANCHFVPRSSKIILPDIILFACV------------------LSHVGCQLSVDCQTFDKEILNIQISVGYDSSATFFNKTYKK 540

HSP 2 Score: 571.622 bits (1472), Expect = 0.000e+0
Identity = 273/279 (97.85%), Postives = 275/279 (98.57%), Query Frame = 1 
Query: 1465 QKTFPTVVFYHPRYPRLNTIITCMTDTENSILQFRWVCNHTDKYLEFMLVDFFKTQGQFMVFLNTAPVVNYSCICIVKIRNFKTGSAITIYQNFTMQLRETSLMFCNMKSIIRSSYSKSEIFLYIIVTTLLFMGFPLLAYIITEYQSINSVHVDHLYRLGIADNAETEEQNKIPSFCFSKHNLGEKQFDLFIHLVERCTKNEEKFRRLKASLDLLLEKNKMFQLAKLKYCFNHHEINEHGEQSELHPERILTKKSHMTFEEKKCSSTHFLERIFVRKYM 2301
            + +FPTVVFYHPRYPRLNTIITCMTDTENSILQFRWVCNHTDKYLEFMLVDFFKTQGQFMVFLNTAPVVNYSCICIVKIRNFKTGSAITIYQNFTMQLRETSLMFCNMKSIIRSSYSKSEIFLYIIVTTLLFMGFPLLAYIITEYQSINSVHVDHLYRLGIADNAETEEQNKIPSFCFSKHNLGEKQFDLFIHLVERCTKNEEKFRRLKASLDLLLEKNKMFQLAKLKYCFNHHEINEHGEQSELHPERILTKKSHMTFEEKKCSSTHFLERIF  K M
Sbjct:  540 KASFPTVVFYHPRYPRLNTIITCMTDTENSILQFRWVCNHTDKYLEFMLVDFFKTQGQFMVFLNTAPVVNYSCICIVKIRNFKTGSAITIYQNFTMQLRETSLMFCNMKSIIRSSYSKSEIFLYIIVTTLLFMGFPLLAYIITEYQSINSVHVDHLYRLGIADNAETEEQNKIPSFCFSKHNLGEKQFDLFIHLVERCTKNEEKFRRLKASLDLLLEKNKMFQLAKLKYCFNHHEINEHGEQSELHPERILTKKSHMTFEEKKCSSTHFLERIFQMKSM 818

HSP 3 Score: 420.239 bits (1079), Expect = 2.931e-136
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 2 
Query: 2459 SDFSKPLVDMYFNSPVKPRRSVTQIMNFKTPRLLSRTNFXXXXXXXXXXXKNEKKPIIRNLETEHSKSSEKKPIINNAEDDHSKSTQFLEKILQMKAMKDHSKSEYNLAYRENSAQSRPRIGHVDSDISRRLDLYFNPPRKYRRSATQVLDIKTSNLKSKTNFSIEPLDSSNFLKNEENLVVSNSDDEYLKSNFQYGTLFYPASNPELFPTKTRNENL 3112
            SDFSKPLVDMYFNSPVKPRRSVTQIMNFKTPRLLSRTNFSAESLAALSLLKNEKKPIIRNLETEHSKSSEKKPIINNAEDDHSKSTQFLEKILQMKAMKDHSKSEYNLAYRENSAQSRPRIGHVDSDISRRLDLYFNPPRKYRRSATQVLDIKTSNLKSKTNFSIEPLDSSNFLKNEENLVVSNSDDEYLKSNFQYGTLFYPASNPELFPTKTRNENL
Sbjct:  857 SDFSKPLVDMYFNSPVKPRRSVTQIMNFKTPRLLSRTNFSAESLAALSLLKNEKKPIIRNLETEHSKSSEKKPIINNAEDDHSKSTQFLEKILQMKAMKDHSKSEYNLAYRENSAQSRPRIGHVDSDISRRLDLYFNPPRKYRRSATQVLDIKTSNLKSKTNFSIEPLDSSNFLKNEENLVVSNSDDEYLKSNFQYGTLFYPASNPELFPTKTRNENL 1074

HSP 4 Score: 86.6557 bits (213), Expect = 2.931e-136
Identity = 41/41 (100.00%), Postives = 41/41 (100.00%), Query Frame = 3 
Query: 2334 QMKSMSDHSKNEYHYAYRENRLKSNVEEIFNQVERNPRISN 2456
            QMKSMSDHSKNEYHYAYRENRLKSNVEEIFNQVERNPRISN
Sbjct:  814 QMKSMSDHSKNEYHYAYRENRLKSNVEEIFNQVERNPRISN 854

HSP 5 Score: 106.301 bits (264), Expect = 6.682e-23
Identity = 65/149 (43.62%), Postives = 90/149 (60.40%), Query Frame = 2 
Query: 2648 EHSKSSEKKP--IIN-----NAEDDHSKSTQFLEKILQMKAMKDHSKSEYNLAYRENSAQS-----------RPRIGHVDSDISRRL-DLYFNPPRKYRRSATQVLDIKTSNLKSKTNFSIEPLDSSNFLKNEENLVVSNSDDEYLKSN 3037
            EH + SE  P  I+        E+    ST FLE+I QMK+M DHSK+EY+ AYREN  +S            PRI +++SD S+ L D+YFN P K RRS TQ+++ KT  L S+TNFS E L + + LKNE+  ++ N + E+ KS+
Sbjct:  777 EHGEQSELHPERILTKKSHMTFEEKKCSSTHFLERIFQMKSMSDHSKNEYHYAYRENRLKSNVEEIFNQVERNPRISNIESDFSKPLVDMYFNSPVKPRRSVTQIMNFKTPRLLSRTNFSAESLAALSLLKNEKKPIIRNLETEHSKSS 925
[Close]
BLAST of SMED30001625 vs. Planmine SMEST
Match: SMESG000044313.1 (SMESG000044313.1)

HSP 1 Score: 58.5362 bits (140), Expect = 2.828e-8
Identity = 21/71 (29.58%), Postives = 40/71 (56.34%), Query Frame = 1 
Query: 1477 PTVVFYHPRYPRLNTIITCMTDTENSILQFRWVCNHTDKYLEFMLVDFFKTQGQFMVFLNTAPVVNYSCIC 1689
            P++V    RYP+    + C+TD  + +  F W CN +++  + ++VD+      F+ F  T+P++ + CIC
Sbjct:  492 PSIVMITSRYPKFGERVQCLTDGIDHLYAFSWACNTSNEPRDLVVVDYITFDNAFLTFHETSPLIKFHCIC 562
[Close]
BLAST of SMED30001625 vs. Planmine SMEST
Match: SMESG000044313.1 (SMESG000044313.1)

HSP 1 Score: 58.151 bits (139), Expect = 2.999e-8
Identity = 21/71 (29.58%), Postives = 40/71 (56.34%), Query Frame = 1 
Query: 1477 PTVVFYHPRYPRLNTIITCMTDTENSILQFRWVCNHTDKYLEFMLVDFFKTQGQFMVFLNTAPVVNYSCIC 1689
            P++V    RYP+    + C+TD  + +  F W CN +++  + ++VD+      F+ F  T+P++ + CIC
Sbjct:  442 PSIVMITSRYPKFGERVQCLTDGIDHLYAFSWACNTSNEPRDLVVVDYITFDNAFLTFHETSPLIKFHCIC 512
The following BLAST results are available for this feature:
BLAST of SMED30001625 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001625 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001625 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001625 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001625 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001625 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001625 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001625 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001625 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001625 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001625 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001625 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001625 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001625 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000044311.11.158e-2395.42SMESG000044311.1[more]
SMESG000044311.16.848e-2393.54SMESG000044311.1[more]
SMESG000044311.12.931e-13695.47SMESG000044311.1[more]
SMESG000044313.12.828e-829.58SMESG000044313.1[more]
SMESG000044313.12.999e-829.58SMESG000044313.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001625 ID=SMED30001625|Name=SMED30001625|organism=Schmidtea mediterranea sexual|type=transcript|length=3200bp
TGGTGAGAATTAATGTTTATTATGAAATGAGTTAATTGTTTATGAAGGGT
TTCATGAGAGGATTATTGACAGTGATTCGTACGGAGTATATATTGACCAA
GATATTCAGAATTTTAATATCGAAAACAAATTTATATTTAAAGTCGTCCC
TAAAGTTTTCAACAGTTCCTTGAAAATTATCGCAGATAATTTTACGAATG
TCTTTTATAATAGAACCACTGGCATTGTGATATGCACCTTTCGATACGAC
CGCAGAGATATTAATTGGGATAAAAGAATAGTAGTAGAAGGATTGAATAA
CAAAAGCATCATTATTGTCAAGTTTAAAGACAAAGAGAAAGGAATAAAAA
TTCATTGCCCAACTCAATATATTGTTCGATCTGCAGTCCAGCAAGCCTTT
TGCACTCCGTACCTAGCGGAGGAAAAGGAGACTGATATGTTTCGCCATAG
AAAGTATCCAATTACTTATACATATTTCTATGGCCATAAAGTCCAACCGG
CGATTTACACGGATGATATTGTTGAAAAAAATAAATTCGAGTATAAAATC
CGGGTAGAAATGACAATCAGACGAGTAGCAGATTTGATTTCTAAAACAAC
AGAAGTAAAATTAATTTTGTTTGGAATTCCTTTTTCCCCTTTTACCAATT
ACAACTGGTCATTTCCGCATCGAATAAATATAAAAAAAGAGAAACTGGTG
TTTATTTTATCAAGTTCCGAAATATATCTGGATCATTTTGTTATTTTCAC
TGTTAATGGACTTCGTTTAAAATTTCAAAATATTACAATAACGAAACAAT
ATTCTGATTTTCCGTCTCTACCCGAGTATTTTGATATCAAAGTAACCGGA
TACATTTTAATAAAATTAAATTCAATGCCCGAAAAGTACCAGATTTTGAA
TGGAGTAATTTCATTTTTGCTTTACGAAACGACAGATTACTTTTTTCCTA
TTACCCATGTTTTCTCTACAGTAGCTCTAAATTGTCCGACGAAATCTAAG
ATAAATTCTAAAAGGATACAAATAAAAATGCAATTTCCAAAATTATACAG
TTCATTCGTAATTAGTAATAAAACTGATCTGATAGTTTCAAACCGGTGGA
AAGAAGGTTCTCATGCTATTGTCAAATGCCTAGCCTTAATGGCTCCTAGT
CTGACGACAGTATTTAAAGACCCCAATCATAGGATATTGCAAGTAAAGGA
TGTTAAGATAATTAAAAAAGACATAGCAAATTGTCACTTCGTGCCTAGGT
CATCGAAAATAATCCTTCCTGATATCATACTATTCGCATGCGTTTGTCAC
GCGTCCGCCAAAATTGTACATATAATAACATTTCCTGTTTGTAAGATAGT
ATCTCATGTTGGTTGTCAGTTAAGCGTTGACTGTCAAACTTTTGACAAAG
AGATTTTAAACATTCAGATTTCGGTTGGATATGATAGCTCAGCAACCTTT
TTCAATAAAACCTACAAAAAACGTTTCCTACAGTCGTGTTTTACCATCCA
AGGTATCCGAGGCTAAACACTATCATAACGTGCATGACTGATACCGAGAA
CAGCATCCTTCAATTTCGATGGGTTTGCAACCATACAGACAAATATCTGG
AATTTATGTTAGTAGACTTCTTCAAAACGCAAGGTCAGTTTATGGTATTT
TTAAATACGGCTCCTGTGGTGAATTACAGTTGCATATGCATTGTGAAAAT
TAGGAATTTCAAAACTGGGTCTGCAATTACAATTTACCAAAACTTTACTA
TGCAACTGCGAGAGACCAGTCTTATGTTTTGTAACATGAAATCCATTATC
CGATCATCATATAGTAAATCCGAAATATTTCTATATATTATTGTGACAAC
ATTGTTGTTCATGGGTTTCCCACTTCTAGCATACATTATTACTGAATATC
AGTCCATTAATTCAGTACATGTGGACCATCTATACAGATTAGGCATTGCG
GATAATGCAGAAACGGAGGAGCAGAATAAAATCCCTTCGTTTTGCTTTTC
AAAACACAATTTAGGAGAAAAACAATTTGATTTATTTATTCATTTAGTTG
AAAGATGCACAAAAAATGAAGAAAAATTTAGAAGATTAAAAGCTAGCTTG
GACCTTTTGTTGGAAAAAAATAAAATGTTTCAATTGGCAAAATTAAAATA
TTGTTTTAATCATCATGAAATAAATGAACATGGTGAACAATCAGAACTCC
ATCCGGAAAGAATTTTGACGAAAAAGTCACACATGACATTTGAGGAAAAG
AAATGTTCATCGACCCATTTTCTCGAAAGGATATTCGTAAGGAAATATAT
GGTGATTATTTGAATATTTTCCTAAATTTTCAGCAAATGAAATCAATGAG
TGACCACTCAAAAAATGAATACCATTATGCATATCGAGAAAATCGTTTGA
AAAGTAATGTAGAAGAAATATTCAACCAGGTAGAAAGGAATCCAAGAATA
AGTAATAAAGTGATTTCTCTAAACCACTAGTAGATATGTATTTTAACTCG
CCTGTCAAACCAAGAAGATCGGTTACACAAATAATGAATTTCAAAACTCC
AAGACTACTATCAAGGACAAATTTCTCAGCAGAAAGCTTGGCAGCTTTAA
GTTTACTTAAAAATGAAAAGAAACCAATAATTAGGAATCTTGAAACTGAA
CATTCAAAATCGAGTGAAAAAAAACCAATTATCAACAATGCGGAAGATGA
CCATTCCAAATCGACTCAATTCCTCGAAAAGATACTCCAAATGAAAGCAA
TGAAGGACCACTCGAAAAGCGAATACAATTTGGCATATCGGGAAAATTCA
GCTCAAAGCAGACCAAGAATAGGTCATGTAGATAGTGATATATCAAGACG
ACTAGATCTTTATTTTAATCCGCCCCGAAAATACAGACGCTCGGCTACTC
AAGTACTAGATATCAAAACTTCCAACCTTAAATCAAAAACAAATTTTTCA
ATAGAACCTTTGGATTCTTCAAATTTCCTTAAAAATGAAGAAAATCTAGT
AGTCAGCAATTCGGATGATGAATATTTAAAATCGAATTTTCAATATGGCA
CCCTTTTTTATCCAGCCTCCAATCCAGAGCTGTTTCCAACTAAAACCAGA
AATGAAAATTTATAAAAAAATTTTCTTCTGGATTTAATTTCCAGATAAAT
GATGTTCTAAAGAAAAGTGGACTAAACTGAATATTTTATGGACATTACTC
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protein sequence of SMED30001625-orf-1

>SMED30001625-orf-1 ID=SMED30001625-orf-1|Name=SMED30001625-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=260bp
MTDTENSILQFRWVCNHTDKYLEFMLVDFFKTQGQFMVFLNTAPVVNYSC
ICIVKIRNFKTGSAITIYQNFTMQLRETSLMFCNMKSIIRSSYSKSEIFL
YIIVTTLLFMGFPLLAYIITEYQSINSVHVDHLYRLGIADNAETEEQNKI
PSFCFSKHNLGEKQFDLFIHLVERCTKNEEKFRRLKASLDLLLEKNKMFQ
LAKLKYCFNHHEINEHGEQSELHPERILTKKSHMTFEEKKCSSTHFLERI
FVRKYMVII*
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protein sequence of SMED30001625-orf-2

>SMED30001625-orf-2 ID=SMED30001625-orf-2|Name=SMED30001625-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=210bp
MYFNSPVKPRRSVTQIMNFKTPRLLSRTNFSAESLAALSLLKNEKKPIIR
NLETEHSKSSEKKPIINNAEDDHSKSTQFLEKILQMKAMKDHSKSEYNLA
YRENSAQSRPRIGHVDSDISRRLDLYFNPPRKYRRSATQVLDIKTSNLKS
KTNFSIEPLDSSNFLKNEENLVVSNSDDEYLKSNFQYGTLFYPASNPELF
PTKTRNENL*
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protein sequence of SMED30001625-orf-3

>SMED30001625-orf-3 ID=SMED30001625-orf-3|Name=SMED30001625-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=360bp
MFRHRKYPITYTYFYGHKVQPAIYTDDIVEKNKFEYKIRVEMTIRRVADL
ISKTTEVKLILFGIPFSPFTNYNWSFPHRINIKKEKLVFILSSSEIYLDH
FVIFTVNGLRLKFQNITITKQYSDFPSLPEYFDIKVTGYILIKLNSMPEK
YQILNGVISFLLYETTDYFFPITHVFSTVALNCPTKSKINSKRIQIKMQF
PKLYSSFVISNKTDLIVSNRWKEGSHAIVKCLALMAPSLTTVFKDPNHRI
LQVKDVKIIKKDIANCHFVPRSSKIILPDIILFACVCHASAKIVHIITFP
VCKIVSHVGCQLSVDCQTFDKEILNIQISVGYDSSATFFNKTYKKRFLQS
CFTIQGIRG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000419prepharyngeal region