SMED30001622
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30001622 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Alignments
SMED30001622 aligns in the following genomic locations:
Homology
BLAST of SMED30001622 vs. Planmine SMEST
Match: SMESG000040908.1 (SMESG000040908.1) HSP 1 Score: 813.913 bits (2101), Expect = 0.000e+0 Identity = 506/506 (100.00%), Postives = 506/506 (100.00%), Query Frame = 1 Query: 76 METNYKFDILEKILEFSCLIYFVLGCLTSYSWASKELDYKTLNSRILLFWEPIAAIMGIVNVFHIILIGFFIYXXXXXXXXXXXXXXXXILFYILSIPXXXXXXXXXXXXXXRDFHCQETIGKFSIFLCFGELGXXXXXXXXXXXXXXSKLIIPTKQMAQFFRFLKLITIIGMVNYFFFHIVSQQIYNFISVASNTSIYPKINNRNIPALPFDFDSLRNYMQPLVILFCCICVIYLVIIFRLANANNPSISENDEYIQLIESVHNQQNLSQTSKNSNKSFMKNLQLPPXXXXXXXXXXXXXXXXXXXXNSFLNDKLLYKSGQIISATFASFIYLISIVLLILQIFEMKRWRKNEENFNIVDKILTWIGVCGQLMISTAQISLNIYKFEPASAFLLIEYFFLIVQILLQYVTVTLALDRQCIPPKIKTWQEFICPAVXXXXXXXXXXXXXXXDVINANSQKSFNSYETDMFIQIFGAGFQSFGETLSILQRLHSLLIFGKXXXXXNQ 1593 METNYKFDILEKILEFSCLIYFVLGCLTSYSWASKELDYKTLNSRILLFWEPIAAIMGIVNVFHIILIGFFIYKNSKKKIMKKSHKEINILFYILSIPLLLVSMSYLILLIIRDFHCQETIGKFSIFLCFGELGFFIATIVFITFINISKLIIPTKQMAQFFRFLKLITIIGMVNYFFFHIVSQQIYNFISVASNTSIYPKINNRNIPALPFDFDSLRNYMQPLVILFCCICVIYLVIIFRLANANNPSISENDEYIQLIESVHNQQNLSQTSKNSNKSFMKNLQLPPISLILGILLITSSILIIIIINSFLNDKLLYKSGQIISATFASFIYLISIVLLILQIFEMKRWRKNEENFNIVDKILTWIGVCGQLMISTAQISLNIYKFEPASAFLLIEYFFLIVQILLQYVTVTLALDRQCIPPKIKTWQEFICPAVGILSINLGLLFLSLFDVINANSQKSFNSYETDMFIQIFGAGFQSFGETLSILQRLHSLLIFGKIIIIINQ Sbjct: 1 METNYKFDILEKILEFSCLIYFVLGCLTSYSWASKELDYKTLNSRILLFWEPIAAIMGIVNVFHIILIGFFIYKNSKKKIMKKSHKEINILFYILSIPLLLVSMSYLILLIIRDFHCQETIGKFSIFLCFGELGFFIATIVFITFINISKLIIPTKQMAQFFRFLKLITIIGMVNYFFFHIVSQQIYNFISVASNTSIYPKINNRNIPALPFDFDSLRNYMQPLVILFCCICVIYLVIIFRLANANNPSISENDEYIQLIESVHNQQNLSQTSKNSNKSFMKNLQLPPISLILGILLITSSILIIIIINSFLNDKLLYKSGQIISATFASFIYLISIVLLILQIFEMKRWRKNEENFNIVDKILTWIGVCGQLMISTAQISLNIYKFEPASAFLLIEYFFLIVQILLQYVTVTLALDRQCIPPKIKTWQEFICPAVGILSINLGLLFLSLFDVINANSQKSFNSYETDMFIQIFGAGFQSFGETLSILQRLHSLLIFGKIIIIINQ 506
BLAST of SMED30001622 vs. Planmine SMEST
Match: SMESG000040891.1 (SMESG000040891.1) HSP 1 Score: 265.003 bits (676), Expect = 1.262e-81 Identity = 215/521 (41.27%), Postives = 309/521 (59.31%), Query Frame = 1 Query: 76 METNYKFDILEKILEFSCLIYFVLGCLTSYSWASKELDYKTLNSRILLFWEPIAAIMGIVNVFHIILIGFFIYXXXXXXXXXXXXXXXXILFYILSIPXXXXXXXXXXXXXXRDFHCQETIGKFSIFLCFGELGXXXXXXXXXXXXXXSKLIIPTKQMAQFFRFLKLITIIGMVNYFFFHIVSQQIYNFISVASNTSIYPKI-NNRNIP-ALPFDFDSLRNYMQPLVILFCCICVIYLVIIFRLANANNPSISENDEYIQLIESVHNQQNLSQTSK---------NSNKSFMKNLQLPPXXXXXXXXXXXXXXXXXXXXNSFLNDKLLYKSGQIISATFASFIYLISIVLLILQIFEMKRWRKNEENFNIVDKILTWIGVCGQLMISTAQISLNIYKFEPA-SAFLLIEYFFLIVQILLQYVTVTLALDRQCIPPKIKTWQEFICPAVXXXXX----XXXXXXXXXXDVINANSQKSFNSYETDMFIQIFGAGFQSFGETLSILQRLHSLLIFGKXXXXXN 1590 M YK++I EK+L+ + L YFV G T+Y WA KELD + + +I FW PIA + GI+N F+ IL I K+++ + + E N+LFY+L PL L ++ + IL I F + ++ + GE+ FI T I IN I +M RF+ L + IG++NYF FH+ QQI NFIS+AS+ Y K NN P PF F ++ Y+QPLVILFCC C++YL+ + ++ ++ +N +L E ++ N S+T SN + +LPP+SL+LGILLIT S+L + I+NS L LY +GQ +S F I ++S+ LL +QI EM+RW K ++N NIVD L WIG GQ+ I+ +Q+ L + +P A YF LI+Q +LQ +T++L+LDR+ I P+ K+WQE+ICPAVGILSI + +F+ + DV+N K + T++F+++FG GF S GETL ILQRLHS LIF KIII N Sbjct: 1 MNPEYKYEIFEKLLQLTGLFYFVSGFFTTYCWACKELDKVSPHEQINNFWNPIAVVSGIINCFNFILTIITI-SQGKRQVQTERNIEGNLLFYVLCAPLFLATIVFTILEITV-FSMKLHENDLTMLMSIGEMFLFIGTFFLILNINFKFWIFLGSKMKLLNRFVMLTSFIGIINYFMFHMTDQQITNFISIASDYDAYSKYYNNSTKPREGPFHFSRIKKYVQPLVILFCCTCMVYLIKFYFSRTSSQKNLDDNST--ELSEETLDKINDSETENLIIRENEVVRSNTKNPPSFRLPPLSLVLGILLITVSLLAVAILNSLLKSTSLYFTGQYLSTIFPIPIDILSLFLLYMQIIEMERWPKIKKNENIVDSTLVWIGAIGQITIAFSQLELYTFIRDPGLVAAKDCYYFVLILQTILQTLTISLSLDREWISPERKSWQEYICPAVGILSINISFLIINVFILMGDVLNEQMAK---NNITELFLKMFGNGFSSLGETLGILQRLHSSLIFIKIIIYTN 514
BLAST of SMED30001622 vs. Planmine SMEST
Match: SMESG000020517.1 (SMESG000020517.1) HSP 1 Score: 221.861 bits (564), Expect = 2.650e-65 Identity = 190/512 (37.11%), Postives = 289/512 (56.45%), Query Frame = 1 Query: 88 YKFDILEKILEFSCLIYFVLGCLTSYSWASKELDYKTLNSRILLFWEPIAAIMGIVNVFHIILIGFFIYXXXXXXXXXXXXXXXXILFYILSIPXXXXXXXXXXXXXXRDFHCQETIGKFSIFLCFGELGXXXXXXXXXXXXXXSKLIIPTKQMAQFFRFLKLITIIGMVNYFFFHIVSQQIYNFISVASNTSIYPKINNRNIPALPFDFDS----LRNYMQPLVILFCCICVIYLVIIFRLANANNPSISENDEYIQLIESVHNQQNLSQ----TSKNSNKSF----MKNLQLPPXXXXXXXXXXXXXXXXXXXXNSFLNDKLLYKSGQIISATFASFIYLISIVLLILQIFEMKRWRKNEENFNIVDKILTWIGVCGQLMISTAQISLNIYKFEPA-SAFLLIEYFFLIVQILLQYVTVTLALDRQCIPPKIKTWQEFICPAVXXXXXXXXXXXXXXXDVINANSQKSFNSYETD----MFIQIFGAGFQSFGETLSILQRLHSLLIFGK 1572 +K DI EK+L+F L+YFV+G L +Y W KELD K+ RIL F+ PIA +++ ++ + + KK E NIL Y + IP L ++ Y + +I F +F GE+ FI+ I+ I F SK I+ Q + F+ L+ I G+VN +H+ QQI NFI +AS K N +I P D +S + +++QP VILFC + + YL++ + N+ + I E ++ LI+ ++ + S+ S N SF +++ LPP SL L IL IT + II I N+ +N GQ+IS+ F IYL SI LLI+Q+ ++ RW K + ++D+++ W+G GQ++ AQIS+N Y++ P S +++ EY L Q + Q + ++LALDR+ PK+++W E++CP VG+LS+NL + SLFD+IN KS N + + +F++IF GFQSFG L+ILQRLHS LIFGK Sbjct: 4 FKLDIFEKVLKFYVLLYFVMGFLVNYLWICKELDMKSDFKRILEFYNPIAMATSLMDFVSLVSVLILFKWSVSKKKTDLEKLESNILLYTIVIPTFLATIVYSSVKVILSAMEFSEKNDFILFSVMGEICLFISVIILIQFFLKSKFIVNYFQRI-YIDFVVLLNIFGIVNLICYHMSVQQINNFIKIASKFPTLSKFYNLSISQ-PTDNESSLIKINSFLQPFVILFCFVILSYLILFY---NSAHYEIQETEKL--LIDEINLRTGASEEDIIASSNPTISFSFKVFQSINLPPFSLGLAILFITVTFFIIAIANNMMNSPKFATEGQLISSIFPIPIYLFSIFLLIIQMNDICRWEKLGTDKYLIDRMILWVGATGQVVEIVAQISMNSYQYYPKLSGWIIAEYAILFFQTMFQTLVISLALDRKWSQPKLESWHEYLCPPVGMLSLNLSFMIFSLFDIINT---KSLNVFSVELNPYLFMRIFTNGFQSFGVALAILQRLHSSLIFGK 505
BLAST of SMED30001622 vs. Planmine SMEST
Match: SMESG000040928.1 (SMESG000040928.1) HSP 1 Score: 214.542 bits (545), Expect = 1.328e-62 Identity = 183/505 (36.24%), Postives = 291/505 (57.62%), Query Frame = 1 Query: 88 YKFDILEKILEFSCLIYFVLGCLTSYSWASKELDYKTLNSRILLFWEPIAAIMGIVNVFHIILIGFFIYXXXXXXXXXXXXXXXXILFYILSIPXXXXXXXXXXXXXXRDFHCQETIGKFSIFLCFGELGXXXXXXXXXXXXXXSKLIIPTKQMAQFFRFLKLITIIGMVNYFFFHIVSQQIYNFISVASNTSIYP------KINNRNIPALPFDFDSLRNYMQPLVILFCCICVIYLVIIFRLANANNPSISENDEYIQLI-ESVHNQQNLSQTSKNSNKSFMKNLQLPPXXXXXXXXXXXXXXXXXXXXNSFLNDKLLYKSGQIISATFASFIYLISIVLLILQIFEMKRWRKNEENFNIVDKILTWIGVCGQLM--ISTAQISLNIYKFEPASAFLLIEYFFLIVQILLQYVTVTLALDRQCIPPKIKTWQEFICPAVXXXXXXXXXXXXXXXDVINAN-SQKSFNSYETDMFIQIFGAGFQSFGETLSILQRLHSLLIFGK 1572 Y FD++ L+ L F +G T+Y WA LD T + L+FWEPIA I G++++ ++LI +FI + S + ILF LSIPL + S +L+ I+ D ++G F+ F+ E FFI T+ ++ ++ ++ ++ F+ LI+ IGM YF F+IV+ QI +FI VA N S Y + NRN+ + D L Y+QPL I+FC + YL I + + ++ DE +L +S+ ++ S S NSN S ++P S+ILGIL T ++++++I ++ + + SG+ I+ ++A IY++SI++L++Q+FEMKRW K + N+VD L ++G GQ+ ISTA++ + Y+ + S +I+ LIVQI LQ++ + L+L+R+ IPP +KTWQEFICP+ + NLG S+F+++N N Q+SF +F++IFG GF S TL+ILQR HS +IF K Sbjct: 5 YLFDVIRNWLKLLILFSFTVGIFTNYCWACVLLDQVTPINETLIFWEPIAGINGVMDLSIVLLIVYFI-RVSWNDPTIQVDFYSKILFLFLSIPLFIGSQIHLVWHIVGDVKL-SSVGGFTEFMHVTESVFFIGTMTLNMYMITTRRTFNKTKIKEYKSFILLISAIGMFKYFLFNIVTHQIQSFIKVAENYSFYSYFYNNYSMENRNLDSHSPD---LEIYLQPLTIIFCFVSFSYL-IRSEFSKTLGDTDTQFDECSRLRGDSLSHENRESIESTNSNISNYYYFKIPSFSIILGILFFTMTLILVLIASTLKSSE----SGKTITVSYAFSIYIVSIIILVVQMFEMKRWPKKTKIENVVDPALLYLGTAGQIFVSISTAKLYMYNYQNKEFSILTIIQNLILIVQISLQFLVIRLSLERKWIPPVVKTWQEFICPSFTLFCFNLGYFGFSVFEIMNNNIVQESF-----PLFLKIFGNGFYSISMTLAILQRFHSSIIFYK 494
BLAST of SMED30001622 vs. Planmine SMEST
Match: SMESG000040922.1 (SMESG000040922.1) HSP 1 Score: 186.422 bits (472), Expect = 4.092e-52 Identity = 166/506 (32.81%), Postives = 273/506 (53.95%), Query Frame = 1 Query: 76 METNYKFDILEKILEFSCLIYFVLGCLTSYSWASKELDYKTLNSRILLFWEPIAAIMGIVNVFHIILIGFFIYXXXXXXXXXXXXXXXXILFYILSIPXXXXXXXXXXXXXXRDFHCQETIGKFSIFLCFGELGXXXXXXXXXXXXXXSKLIIPTKQ--MAQFFRFLKLITIIGMVNYFFFHIVSQQIYNFISVASNTSIYPKINNRNIPALPFDFDSLRN---YMQPLVILFCCICVIYLVIIFRLANANNPSISENDEYIQLIESVHNQQNLSQTSKNSNKSFMKNLQLPPXXXXXXXXXXXXXXXXXXXXNSFLNDKLLYKSGQIISATFASFIYLISIVLLILQIFEMKRWRKNEENFNIVDKILTWIGVCGQLMISTAQISLN-IYKFEPASAFLLIEYFFLIVQILLQYVTVTLALDRQCIPPKIKTWQEFICPAVXXXXXXXXXXXXXXXDVINAN-SQKSFNSYETDMFIQIFGAGFQSFGETLSILQRLHSLLIFGK 1572 M +Y D+ E+IL F L YF+LG LT+Y WA LDY + I FW PI A++ ++ I ++ ++ +H E L Y L +P L +++++ + II F++ L F E+ + ++ P + + F+ +IG N + +QQI NF+ VASN S Y + +++ D+ +L N Y+ P+ ILFCC+CVI L L N + +++E+ Q+I + NL + ++ +++L + P+ LIL ++ + SI + IIINSFLN +GQ+IS F Y ++I+LL +QI E+ W+K N VDK++ WIG GQL+I A++ + I + E SA ++ +Y L+VQI +Q V + +AL+R+ + PK+K+WQE + P + +LS+N+G LF+S+ + N N S + + + +F++IFG GFQ G L+I R+HS +IFGK Sbjct: 1 MNESYWSDLFEQILNFFALSYFILGYLTNYLWACNRLDYTSSYKEIFKFWNPI-AVITGISNIIIGILIIIALNVRINRVSIPNHFEYQTLIYTLLVPSFLGTVTFMAIRIILTIKSYMNFNDFTMVLSFSEM--ILFCCGIGLIRKKISMVNPEVNCYLQKLVEFINKSALIGFCNCLIHFMSAQQIDNFVKVASNFSFYFQFYGKSLTNRTVDYYNLSNINSYLVPIAILFCCLCVICLFFNL-LNNKSQNTLTEDQNDSQVIVQWTSTTNLLENEPIFDR--VRSLNMSPLLLILALIFLAISIFLTIIINSFLNSYQYNITGQLISTVFPIPFYFLAIILLAIQINEIMEWKKISTNEFTVDKMMLWIGAAGQLVICFAKLFMEFIERTENFSALIIAKYGLLLVQIFIQTVALNMALNRKWLRPKLKSWQEKLFPPISLLSLNMGFLFISMLNDFNRNTSHSTVKNIDKRLFLRIFGNGFQLPGLILAIFHRMHSSVIFGK 500 The following BLAST results are available for this feature:
BLAST of SMED30001622 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30001622 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30001622 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30001622 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30001622 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30001622 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30001622 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30001622 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30001622 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30001622 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30001622 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30001622 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30001622 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30001622 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30001622 ID=SMED30001622|Name=SMED30001622|organism=Schmidtea mediterranea sexual|type=transcript|length=1702bpback to top protein sequence of SMED30001622-orf-1 >SMED30001622-orf-1 ID=SMED30001622-orf-1|Name=SMED30001622-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=507bp METNYKFDILEKILEFSCLIYFVLGCLTSYSWASKELDYKTLNSRILLFWback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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