secondary spermatocytes accumulation 1 protein

Overview
Namesecondary spermatocytes accumulation 1 protein (ADK78239.1)
Smed IDSMED30001554
Length (bp)1607
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of secondary spermatocytes accumulation 1 protein (SMED30001554) t-SNE clustered cells

Violin plots show distribution of expression levels for secondary spermatocytes accumulation 1 protein (SMED30001554) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30001554

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
testisSMED30001554h1SMcG0010489 Contig4510_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
early male germ cellSMED30001554h1SMcG0010489 Contig4510_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism asexual adult cDNA to DNA expression microarray evidence
testis primordiumSMED30001554h1SMcG0010489 Contig4510_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism juvenile stagecDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of secondary spermatocytes accumulation 1 protein vs. Ensembl Celegans
Match: Y57G11C.9 (pep chromosome:WBcel235:IV:14787168:14790843:1 gene:WBGene00013307.1 transcript:Y57G11C.9a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y57G11C.9)

HSP 1 Score: 69.707 bits (169), Expect = 2.013e-12
Identity = 30/93 (32.26%), Postives = 58/93 (62.37%), Query Frame = 3 
Query:   57 RVDPKMVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRK 335
            R++   V  S+H+IFIRG   ++S E ++ +   E       DF   ++D+ K+F+A+RFE+ ++A + +E +  +E +G   ++SWF+D+R+
Sbjct:   39 RINETQVPQSKHTIFIRGLHGDISTEEIKEYI-GEKVGKISFDFVKVAQDKSKIFVAVRFENRDEAKEFMETYSDREFMGCRCDLSWFRDIRR 130
[Close]
BLAST of secondary spermatocytes accumulation 1 protein vs. Ensembl Celegans
Match: Y57G11C.9 (pep chromosome:WBcel235:IV:14787168:14790843:1 gene:WBGene00013307.1 transcript:Y57G11C.9b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y57G11C.9)

HSP 1 Score: 70.0922 bits (170), Expect = 2.028e-12
Identity = 30/93 (32.26%), Postives = 58/93 (62.37%), Query Frame = 3 
Query:   57 RVDPKMVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRK 335
            R++   V  S+H+IFIRG   ++S E ++ +   E       DF   ++D+ K+F+A+RFE+ ++A + +E +  +E +G   ++SWF+D+R+
Sbjct:   39 RINETQVPQSKHTIFIRGLHGDISTEEIKEYI-GEKVGKISFDFVKVAQDKSKIFVAVRFENRDEAKEFMETYSDREFMGCRCDLSWFRDIRR 130
[Close]
BLAST of secondary spermatocytes accumulation 1 protein vs. Ensembl Xenopus
Match: zdhhc8 (zinc finger DHHC-type containing 8 [Source:Xenbase;Acc:XB-GENE-976023])

HSP 1 Score: 49.6766 bits (117), Expect = 8.267e-6
Identity = 30/77 (38.96%), Postives = 49/77 (63.64%), Query Frame = 3 
Query: 1275 SEFPNLSPEDHVK----VLTKKESLEKTYKKDCETVASVAKLMISKNPTXXXXXXXXXXXXFTDIGEKYIEELRSYI 1493
            SEF + +P  H+     +  K + +E+ Y++DCET   V K++I K+PT+E+ I   L+   ++IGE+ IEEL+ YI
Sbjct:  260 SEFHDANP--HISRSRAIAAKTKEIEEVYRQDCETFGMVVKMLIDKDPTLEKQIQFALRQNLSEIGERCIEELKMYI 334
[Close]
BLAST of secondary spermatocytes accumulation 1 protein vs. Ensembl Xenopus
Match: zdhhc8 (zinc finger DHHC-type containing 8 [Source:Xenbase;Acc:XB-GENE-976023])

HSP 1 Score: 49.6766 bits (117), Expect = 8.294e-6
Identity = 30/77 (38.96%), Postives = 49/77 (63.64%), Query Frame = 3 
Query: 1275 SEFPNLSPEDHV----KVLTKKESLEKTYKKDCETVASVAKLMISKNPTXXXXXXXXXXXXFTDIGEKYIEELRSYI 1493
            SEF + +P  H+     +  K + +E+ Y++DCET   V K++I K+PT+E+ I   L+   ++IGE+ IEEL+ YI
Sbjct:  261 SEFHDANP--HISRSRAIAAKTKEIEEVYRQDCETFGMVVKMLIDKDPTLEKQIQFALRQNLSEIGERCIEELKMYI 335
[Close]
BLAST of secondary spermatocytes accumulation 1 protein vs. TrEMBL
Match: E2IXF9 (Secondary spermatocytes accumulation 1 protein (Fragment) OS=Schmidtea mediterranea OX=79327 GN=spac-1 PE=2 SV=1)

HSP 1 Score: 546.584 bits (1407), Expect = 0.000e+0
Identity = 329/332 (99.10%), Postives = 330/332 (99.40%), Query Frame = 3 
Query:   21 MSSFKNGNNSSDRVDPKMVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRKARKQLIEEERANRNNDSRSPVEWNVMNKNQSGFGRNGMYMWTGRFLRNRGLRNMGNFNRQRDCFQRRMFNTRNTGCWRRARLSPGHPGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNKEIGHDSVHKYXXXXXXXXXXDRQEVKRSESIQEIKDTGNCLKKPESIPKISDSIQSAVAVDVISPNIVHAYSISPTESSASSNEMS 1016
            M+ FKNGNNSSDRVDP MVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRKARKQLIEEERANRNNDSRSPVEWNVMNKNQSGFGRNGMYMWTGRFLRNRGLRNMGNFNRQRDCFQRRMFNTRNTGCWRRARLSPGHPGRTRRERSHSNSRSFSRSRSRSRSRSRSYSSKSHRRSCSSYSSRKSRDKSSSNKEIGHDSVHKYRFNRSNSFSSDRQEVKRSESIQEIKDTGNCLKKPESIPKISDSIQSAVAVDVISPNIVHAYSISPTESSASSNEMS
Sbjct:    1 MTPFKNGNNSSDRVDPTMVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRKARKQLIEEERANRNNDSRSPVEWNVMNKNQSGFGRNGMYMWTGRFLRNRGLRNMGNFNRQRDCFQRRMFNTRNTGCWRRARLSPGHPGRTRRERSHSNSRSFSRSRSRSRSRSRSYSSKSHRRSCSSYSSRKSRDKSSSNKEIGHDSVHKYRFNRSNSFSSDRQEVKRSESIQEIKDTGNCLKKPESIPKISDSIQSAVAVDVISPNIVHAYSISPTESSASSNEMS 332
[Close]
BLAST of secondary spermatocytes accumulation 1 protein vs. TrEMBL
Match: A0A5K3FIE9 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 7.245e-29
Identity = 54/107 (50.47%), Postives = 84/107 (78.50%), Query Frame = 3 
Query:   54 DRVDPKMVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRKARKQLIEEERANR 374
            DRV+P+ V PSRH+IFIRG P + ++++V++ F  ESK  C ++FFS SED+ +  +A+RF+SHE A +++E ++ KE++GH +EV+WFKDL+KAR ++ EE+R NR
Sbjct:    9 DRVNPRYVPPSRHTIFIRGLPGSTNVDSVKNHFSEESKSKCTVEFFSTSEDKVRFSVAIRFKSHETAREMLEKYNGKELMGHPVEVTWFKDLKKARARVYEEQRHNR 115
[Close]
BLAST of secondary spermatocytes accumulation 1 protein vs. TrEMBL
Match: A0A4S2L6S5 (RRM domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_009603 PE=4 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 1.706e-28
Identity = 54/101 (53.47%), Postives = 78/101 (77.23%), Query Frame = 3 
Query:   60 VDPKMVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRKARKQLIEEE 362
            +DP+ V PSRHSIFIRGFP N+S+E+++  F      NCKIDFFS+SED+ +LF+A+RF+SH  A  +I  +D + + G+ +E++WFKD+RKARK++IE E
Sbjct:    9 IDPRKVLPSRHSIFIRGFPGNVSIEDIKELFSKHCDGNCKIDFFSFSEDKSQLFVAIRFDSHSVAKKLISKYDGERIFGYKVELNWFKDIRKARKKVIENE 109
[Close]
BLAST of secondary spermatocytes accumulation 1 protein vs. TrEMBL
Match: A0A419QDQ9 (RRM domain-containing protein OS=Clonorchis sinensis OX=79923 GN=CSKR_10334s PE=4 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 4.797e-28
Identity = 58/139 (41.73%), Postives = 91/139 (65.47%), Query Frame = 3 
Query:   60 VDPKMVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRKARKQLIEEERANRNNDSRSPVEWNVMNKNQSGFGRNGMYMWTGRFLR 476
            +DP+ V PSRHSIFIRGFP N+S+++++  F      NCKIDFFS+SED+ +LF+A+RF+SH  A  +I  +D + + G+ +E++WFKD+RKARK++IE E   +   +    +       +    R G+  + G+FL+
Sbjct:    9 IDPRKVLPSRHSIFIRGFPGNVSIDDIKELFSKHCDGNCKIDFFSFSEDKSQLFVAIRFDSHSVAKRLISKYDGERIFGYKVELNWFKDIRKARKKVIENEYKYQTLLAHPHFDIRRRPSGRFPLRRMGLKRFGGKFLQ 147
[Close]
BLAST of secondary spermatocytes accumulation 1 protein vs. TrEMBL
Match: A0A068YCE1 (Dentin sialophosphoprotein OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000750800 PE=4 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 7.568e-28
Identity = 52/107 (48.60%), Postives = 84/107 (78.50%), Query Frame = 3 
Query:   54 DRVDPKMVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRKARKQLIEEERANR 374
            DRV+P+ V PSRH+IFIRG P + ++++V++ F +E+   C ++FFS SED+ +  +A+RF+SHE A +++E ++ KE+LGH +EV+WFKDL+KAR ++ EE+R +R
Sbjct:    9 DRVNPRFVPPSRHTIFIRGLPGSTNVDSVKNHFSSETSSKCTVEFFSTSEDKVRFSVAIRFKSHETAREMLEKYNGKELLGHPVEVTWFKDLKKARARVYEEQRHSR 115
[Close]
BLAST of secondary spermatocytes accumulation 1 protein vs. Planmine SMEST
Match: SMESG000002926.1 (SMESG000002926.1)

HSP 1 Score: 770 bits (1987), Expect = 0.000e+0
Identity = 503/503 (100.00%), Postives = 503/503 (100.00%), Query Frame = 3 
Query:   21 MSSFKNGNNSSDRVDPKMVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRKARKQLIEEERANRNNDSRSPVEWNVMNKNQSGFGRNGMYMWTGRFLRNRGLRNMGNFNRQRDCFQRRMFNTRNTGCWRRARLSPGHPGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNKEIGHDSVHKYXXXXXXXXXXDRQEVKRSESIQEIKDTGNCLKKPESIPKISDSIQSAVAVDVISPNIVHAYSISPTESSASSNEMSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQRCVKLIQVESKPDNLKIYQDNDLLNAVPIKVVKDDQTDIINIKSEFPNLSPEDHVKVLTKKESLEKTYKKDCETVASVAKLMISKNPTXXXXXXXXXXXXFTDIGEKYIEELRSYINYLANINSQQII 1529
            MSSFKNGNNSSDRVDPKMVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRKARKQLIEEERANRNNDSRSPVEWNVMNKNQSGFGRNGMYMWTGRFLRNRGLRNMGNFNRQRDCFQRRMFNTRNTGCWRRARLSPGHPGRTRRERSHSNSRSFSRSRSRSRSRSRSYSSKSHRRSCSSYSSRKSRDKSSSNKEIGHDSVHKYRFNRSNSFSSDRQEVKRSESIQEIKDTGNCLKKPESIPKISDSIQSAVAVDVISPNIVHAYSISPTESSASSNEMSNKLEKFNNKKHKRKSKKKNKKRKLNKKSKDSDDNSSADSYDDQRCVKLIQVESKPDNLKIYQDNDLLNAVPIKVVKDDQTDIINIKSEFPNLSPEDHVKVLTKKESLEKTYKKDCETVASVAKLMISKNPTIEESILIILKSIFTDIGEKYIEELRSYINYLANINSQQII
Sbjct:    1 MSSFKNGNNSSDRVDPKMVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRKARKQLIEEERANRNNDSRSPVEWNVMNKNQSGFGRNGMYMWTGRFLRNRGLRNMGNFNRQRDCFQRRMFNTRNTGCWRRARLSPGHPGRTRRERSHSNSRSFSRSRSRSRSRSRSYSSKSHRRSCSSYSSRKSRDKSSSNKEIGHDSVHKYRFNRSNSFSSDRQEVKRSESIQEIKDTGNCLKKPESIPKISDSIQSAVAVDVISPNIVHAYSISPTESSASSNEMSNKLEKFNNKKHKRKSKKKNKKRKLNKKSKDSDDNSSADSYDDQRCVKLIQVESKPDNLKIYQDNDLLNAVPIKVVKDDQTDIINIKSEFPNLSPEDHVKVLTKKESLEKTYKKDCETVASVAKLMISKNPTIEESILIILKSIFTDIGEKYIEELRSYINYLANINSQQII 503
[Close]
BLAST of secondary spermatocytes accumulation 1 protein vs. Planmine SMEST
Match: SMESG000002926.1 (SMESG000002926.1)

HSP 1 Score: 736.487 bits (1900), Expect = 0.000e+0
Identity = 486/486 (100.00%), Postives = 486/486 (100.00%), Query Frame = 3 
Query:   72 MVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRKARKQLIEEERANRNNDSRSPVEWNVMNKNQSGFGRNGMYMWTGRFLRNRGLRNMGNFNRQRDCFQRRMFNTRNTGCWRRARLSPGHPGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNKEIGHDSVHKYXXXXXXXXXXDRQEVKRSESIQEIKDTGNCLKKPESIPKISDSIQSAVAVDVISPNIVHAYSISPTESSASSNEMSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQRCVKLIQVESKPDNLKIYQDNDLLNAVPIKVVKDDQTDIINIKSEFPNLSPEDHVKVLTKKESLEKTYKKDCETVASVAKLMISKNPTXXXXXXXXXXXXFTDIGEKYIEELRSYINYLANINSQQII 1529
            MVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRKARKQLIEEERANRNNDSRSPVEWNVMNKNQSGFGRNGMYMWTGRFLRNRGLRNMGNFNRQRDCFQRRMFNTRNTGCWRRARLSPGHPGRTRRERSHSNSRSFSRSRSRSRSRSRSYSSKSHRRSCSSYSSRKSRDKSSSNKEIGHDSVHKYRFNRSNSFSSDRQEVKRSESIQEIKDTGNCLKKPESIPKISDSIQSAVAVDVISPNIVHAYSISPTESSASSNEMSNKLEKFNNKKHKRKSKKKNKKRKLNKKSKDSDDNSSADSYDDQRCVKLIQVESKPDNLKIYQDNDLLNAVPIKVVKDDQTDIINIKSEFPNLSPEDHVKVLTKKESLEKTYKKDCETVASVAKLMISKNPTIEESILIILKSIFTDIGEKYIEELRSYINYLANINSQQII
Sbjct:    1 MVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRKARKQLIEEERANRNNDSRSPVEWNVMNKNQSGFGRNGMYMWTGRFLRNRGLRNMGNFNRQRDCFQRRMFNTRNTGCWRRARLSPGHPGRTRRERSHSNSRSFSRSRSRSRSRSRSYSSKSHRRSCSSYSSRKSRDKSSSNKEIGHDSVHKYRFNRSNSFSSDRQEVKRSESIQEIKDTGNCLKKPESIPKISDSIQSAVAVDVISPNIVHAYSISPTESSASSNEMSNKLEKFNNKKHKRKSKKKNKKRKLNKKSKDSDDNSSADSYDDQRCVKLIQVESKPDNLKIYQDNDLLNAVPIKVVKDDQTDIINIKSEFPNLSPEDHVKVLTKKESLEKTYKKDCETVASVAKLMISKNPTIEESILIILKSIFTDIGEKYIEELRSYINYLANINSQQII 486
[Close]
BLAST of secondary spermatocytes accumulation 1 protein vs. Planmine SMEST
Match: SMESG000038824.1 (SMESG000038824.1)

HSP 1 Score: 127.102 bits (318), Expect = 1.683e-31
Identity = 55/107 (51.40%), Postives = 83/107 (77.57%), Query Frame = 3 
Query:   45 NSSDRVDPKMVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRKARKQLIEEER 365
            NS +RV+PK V PSRH+IFIRGF +++    +R FF+  SK NC +DF SY+++++KL IALRFESH+ A D++  F++ E+ G+ +EV+WFKDL+KAR ++++E R
Sbjct:   19 NSFERVNPKYVPPSRHAIFIRGFANDVDTHKIRDFFQEASKSNCGVDFTSYNDEQRKLSIALRFESHDIAKDMLLRFNNTEIFGYRVEVTWFKDLKKARDRMLDERR 125
[Close]
BLAST of secondary spermatocytes accumulation 1 protein vs. Planmine SMEST
Match: SMESG000038824.1 (SMESG000038824.1)

HSP 1 Score: 127.102 bits (318), Expect = 1.760e-31
Identity = 55/107 (51.40%), Postives = 83/107 (77.57%), Query Frame = 3 
Query:   45 NSSDRVDPKMVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRKARKQLIEEER 365
            NS +RV+PK V PSRH+IFIRGF +++    +R FF+  SK NC +DF SY+++++KL IALRFESH+ A D++  F++ E+ G+ +EV+WFKDL+KAR ++++E R
Sbjct:   19 NSFERVNPKYVPPSRHAIFIRGFANDVDTHKIRDFFQEASKSNCGVDFTSYNDEQRKLSIALRFESHDIAKDMLLRFNNTEIFGYRVEVTWFKDLKKARDRMLDERR 125
[Close]
BLAST of secondary spermatocytes accumulation 1 protein vs. Planmine SMEST
Match: SMESG000038824.1 (SMESG000038824.1)

HSP 1 Score: 125.561 bits (314), Expect = 3.504e-31
Identity = 55/108 (50.93%), Postives = 83/108 (76.85%), Query Frame = 3 
Query:   45 NSSDRVDPKMVTPSRHSIFIRGFPSNMSMENVRSFFKTESKENCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWFKDLRKARKQLIEEERA 368
            NS +RV+PK V PSRH+IFIRGF +++    +R FF+  SK NC +DF SY+++++KL IALRFESH+ A D++  F++ E+ G+ +EV+WFKDL+KAR ++++E R 
Sbjct:   19 NSFERVNPKYVPPSRHAIFIRGFANDVDTHKIRDFFQEASKSNCGVDFTSYNDEQRKLSIALRFESHDIAKDMLLRFNNTEIFGYRVEVTWFKDLKKARDRMLDERRV 126
The following BLAST results are available for this feature:
BLAST of secondary spermatocytes accumulation 1 protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of secondary spermatocytes accumulation 1 protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Y57G11C.92.013e-1232.26pep chromosome:WBcel235:IV:14787168:14790843:1 gen... [more]
Y57G11C.92.028e-1232.26pep chromosome:WBcel235:IV:14787168:14790843:1 gen... [more]
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BLAST of secondary spermatocytes accumulation 1 protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of secondary spermatocytes accumulation 1 protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of secondary spermatocytes accumulation 1 protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
zdhhc88.267e-638.96zinc finger DHHC-type containing 8 [Source:Xenbase... [more]
zdhhc88.294e-638.96zinc finger DHHC-type containing 8 [Source:Xenbase... [more]
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BLAST of secondary spermatocytes accumulation 1 protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of secondary spermatocytes accumulation 1 protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of secondary spermatocytes accumulation 1 protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
E2IXF90.000e+099.10Secondary spermatocytes accumulation 1 protein (Fr... [more]
A0A5K3FIE97.245e-2950.47Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A4S2L6S51.706e-2853.47RRM domain-containing protein OS=Opisthorchis feli... [more]
A0A419QDQ94.797e-2841.73RRM domain-containing protein OS=Clonorchis sinens... [more]
A0A068YCE17.568e-2848.60Dentin sialophosphoprotein OS=Echinococcus multilo... [more]
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BLAST of secondary spermatocytes accumulation 1 protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of secondary spermatocytes accumulation 1 protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of secondary spermatocytes accumulation 1 protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of secondary spermatocytes accumulation 1 protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of secondary spermatocytes accumulation 1 protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of secondary spermatocytes accumulation 1 protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000002926.10.000e+0100.00SMESG000002926.1[more]
SMESG000002926.10.000e+0100.00SMESG000002926.1[more]
SMESG000038824.11.683e-3151.40SMESG000038824.1[more]
SMESG000038824.11.760e-3151.40SMESG000038824.1[more]
SMESG000038824.13.504e-3150.93SMESG000038824.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30001554
GenBankADK78239.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001554 ID=SMED30001554|Name=secondary spermatocytes accumulation 1 protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1607bp
ATTAATTTGAATTGCATCAAATGAGTTCGTTCAAAAATGGAAATAACTCA
AGTGATCGTGTAGATCCCAAAATGGTCACCCCAAGTAGGCATTCTATATT
CATAAGAGGATTTCCGAGCAATATGTCAATGGAAAACGTTAGAAGTTTTT
TCAAAACAGAATCGAAAGAAAATTGTAAAATTGACTTTTTCAGCTATTCG
GAAGATAGGAAAAAATTATTTATCGCATTGAGATTCGAATCTCATGAGAA
GGCTGTCGATATTATAGAAAATTTTGACAGCAAAGAAGTTTTAGGGCATT
CAATTGAGGTATCGTGGTTTAAGGATTTAAGGAAGGCTAGAAAACAATTA
ATTGAAGAGGAGAGAGCAAACAGAAATAATGATTCCAGAAGCCCTGTGGA
ATGGAATGTAATGAACAAAAATCAATCTGGGTTTGGAAGAAATGGAATGT
ACATGTGGACCGGTAGATTTCTACGAAATAGAGGACTTCGAAATATGGGA
AATTTCAATCGTCAAAGGGATTGTTTTCAGAGACGAATGTTCAATACGCG
CAATACCGGCTGTTGGCGTAGGGCTAGGCTGAGCCCTGGTCATCCAGGAA
GAACTCGAAGAGAGAGAAGTCATTCTAATTCAAGATCGTTTTCTAGAAGT
CGCTCTAGAAGCCGATCAAGATCAAGATCATATTCTTCCAAGAGTCATAG
GCGATCATGTAGTTCTTATTCTTCGAGAAAATCTCGAGATAAATCATCGT
CCAACAAAGAGATTGGTCATGATTCAGTGCATAAATACCGATTTAACCGG
TCAAATAGTTTTTCCTCAGACCGACAAGAAGTAAAACGTTCCGAGTCTAT
TCAGGAAATAAAAGATACCGGGAACTGTTTAAAGAAACCTGAGTCAATTC
CAAAGATATCTGATAGTATTCAATCCGCTGTTGCAGTTGATGTCATTTCA
CCGAATATTGTTCATGCGTACTCCATTTCACCTACTGAATCTTCGGCGTC
TTCAAATGAAATGTCAAACAAGTTGGAAAAATTTAATAATAAAAAACACA
AACGAAAGTCAAAGAAAAAAAACAAAAAACGGAAATTAAACAAAAAATCC
AAAGATAGTGATGATAACAGTTCAGCAGACAGTTATGACGACCAAAGGTG
CGTCAAACTGATTCAGGTCGAGTCCAAACCGGATAACCTGAAAATATACC
AAGACAATGATTTATTAAATGCAGTTCCAATAAAAGTGGTCAAAGATGAT
CAGACCGATATTATTAATATTAAGTCTGAATTTCCTAATCTTTCACCAGA
GGATCATGTGAAAGTTTTGACGAAGAAAGAATCTCTAGAAAAGACTTACA
AAAAAGATTGCGAAACGGTAGCATCAGTTGCCAAATTGATGATAAGCAAA
AATCCTACTATTGAAGAAAGTATTCTCATTATATTGAAGTCCATTTTCAC
CGATATTGGGGAGAAATACATTGAGGAATTGAGATCCTATATCAACTATT
TGGCCAATATCAATTCTCAGCAAATTATTTAATGTTTTCTCAAGTTGATA
ATTGTTTTATGGTTCAATTTTATGTTCATTTCTAATAAAATTTTGTAACA
AAAAAAA
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protein sequence of SMED30001554-orf-1

>SMED30001554-orf-1 ID=SMED30001554-orf-1|Name=SMED30001554-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=504bp
MSSFKNGNNSSDRVDPKMVTPSRHSIFIRGFPSNMSMENVRSFFKTESKE
NCKIDFFSYSEDRKKLFIALRFESHEKAVDIIENFDSKEVLGHSIEVSWF
KDLRKARKQLIEEERANRNNDSRSPVEWNVMNKNQSGFGRNGMYMWTGRF
LRNRGLRNMGNFNRQRDCFQRRMFNTRNTGCWRRARLSPGHPGRTRRERS
HSNSRSFSRSRSRSRSRSRSYSSKSHRRSCSSYSSRKSRDKSSSNKEIGH
DSVHKYRFNRSNSFSSDRQEVKRSESIQEIKDTGNCLKKPESIPKISDSI
QSAVAVDVISPNIVHAYSISPTESSASSNEMSNKLEKFNNKKHKRKSKKK
NKKRKLNKKSKDSDDNSSADSYDDQRCVKLIQVESKPDNLKIYQDNDLLN
AVPIKVVKDDQTDIINIKSEFPNLSPEDHVKVLTKKESLEKTYKKDCETV
ASVAKLMISKNPTIEESILIILKSIFTDIGEKYIEELRSYINYLANINSQ
QII*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003723RNA binding
GO:0003676nucleic acid binding
GO:0000166nucleotide binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000208testis
PLANA:0002030early male germ cell
PLANA:0003304testis primordium
Vocabulary: INTERPRO
TermDefinition
IPR028851Pphln1
IPR035979RBD_domain_sf
IPR000504RRM_dom
IPR012677Nucleotide-bd_a/b_plait_sf
Vocabulary: biological process
TermDefinition
GO:0045892negative regulation of transcription, DNA-templated
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 25..98
e-value: 0.0082
score: 25.2
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 26..95
e-value: 9.7E-5
score: 22.1
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 24..102
score: 9.831
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3DG3DSA:3.30.70.330coord: 11..99
e-value: 1.4E-8
score: 36.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 321..371
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 191..205
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 206..224
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 184..277
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 233..257
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 338..357
NoneNo IPR availableCDDcd00590RRM_SFcoord: 26..98
e-value: 1.90857E-5
score: 40.7513
IPR028851Periphilin-1PANTHERPTHR15836PERIPHILIN 1coord: 320..497
IPR035979RNA-binding domain superfamilySUPERFAMILYSSF54928RNA-binding domain, RBDcoord: 21..106