Poly(U)-binding-splicing factor PUF60
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30001529 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Human
Match: PUF60 (poly(U) binding splicing factor 60 [Source:HGNC Symbol;Acc:HGNC:17042]) HSP 1 Score: 258.07 bits (658), Expect = 3.638e-81 Identity = 131/258 (50.78%), Postives = 183/258 (70.93%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK----RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
L L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA +NRIYVA+VH +LS +D++ VFEAFG I+SC L D T GKH+ +GFIEY+ Q++ +A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 53 LGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTT-GKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGL 300
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Human
Match: PUF60 (poly(U) binding splicing factor 60 [Source:HGNC Symbol;Acc:HGNC:17042]) HSP 1 Score: 257.684 bits (657), Expect = 4.209e-81 Identity = 131/258 (50.78%), Postives = 183/258 (70.93%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK----RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
L L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA +NRIYVA+VH +LS +D++ VFEAFG I+SC L D T GKH+ +GFIEY+ Q++ +A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 45 LGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTT-GKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGL 292
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Human
Match: PUF60 (poly(U) binding splicing factor 60 [Source:HGNC Symbol;Acc:HGNC:17042]) HSP 1 Score: 258.455 bits (659), Expect = 4.153e-79 Identity = 131/258 (50.78%), Postives = 183/258 (70.93%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK----RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
L L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA +NRIYVA+VH +LS +D++ VFEAFG I+SC L D T GKH+ +GFIEY+ Q++ +A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 11 LGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTT-GKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGL 258 HSP 2 Score: 96.6709 bits (239), Expect = 1.380e-20 Identity = 62/124 (50.00%), Postives = 79/124 (63.71%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
E +++ I G AR+ +MQKLLR VMVLRNM+D DIDD +E EV EEC KFG V V I E + + ++A VKIFV F + AI ++NGR+F GR V+A+ YDQE FD D SA
Sbjct: 376 EQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA 499
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Human
Match: PUF60 (poly(U) binding splicing factor 60 [Source:HGNC Symbol;Acc:HGNC:17042]) HSP 1 Score: 258.455 bits (659), Expect = 4.153e-79 Identity = 131/258 (50.78%), Postives = 183/258 (70.93%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK----RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
L L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA +NRIYVA+VH +LS +D++ VFEAFG I+SC L D T GKH+ +GFIEY+ Q++ +A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 11 LGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTT-GKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGL 258 HSP 2 Score: 96.6709 bits (239), Expect = 1.380e-20 Identity = 62/124 (50.00%), Postives = 79/124 (63.71%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
E +++ I G AR+ +MQKLLR VMVLRNM+D DIDD +E EV EEC KFG V V I E + + ++A VKIFV F + AI ++NGR+F GR V+A+ YDQE FD D SA
Sbjct: 376 EQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA 499
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Human
Match: PUF60 (poly(U) binding splicing factor 60 [Source:HGNC Symbol;Acc:HGNC:17042]) HSP 1 Score: 258.455 bits (659), Expect = 4.801e-79 Identity = 131/258 (50.78%), Postives = 183/258 (70.93%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK----RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
L L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA +NRIYVA+VH +LS +D++ VFEAFG I+SC L D T GKH+ +GFIEY+ Q++ +A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 17 LGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTT-GKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGL 264 HSP 2 Score: 96.6709 bits (239), Expect = 1.435e-20 Identity = 62/124 (50.00%), Postives = 79/124 (63.71%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
E +++ I G AR+ +MQKLLR VMVLRNM+D DIDD +E EV EEC KFG V V I E + + ++A VKIFV F + AI ++NGR+F GR V+A+ YDQE FD D SA
Sbjct: 382 EQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA 505
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Celegans
Match: rnp-6 (RNP (RRM RNA binding domain) containing [Source:UniProtKB/TrEMBL;Acc:Q9N3S4]) HSP 1 Score: 216.083 bits (549), Expect = 2.689e-65 Identity = 116/266 (43.61%), Postives = 166/266 (62.41%), Query Frame = 1
Query: 94 LPVLEQYGSILEGVGSNKLLEP--FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPN 885
+PVL Q G + G G K + L +L+S KD I AKKYAM+ SI+ +LL+Q KQ + Q +QA+ ++SRIYVGSI++++RE+ +R+A+ FGP+KS+++S+DP T H +AF+E+E PEAA LA + S ++ P NMP + P++D + +A KY R+YV++VHP+LS DL+ VFEAFG I C L T G HR FG++E+ + EAI MN+FDLGGQ LRV K +TPP+
Sbjct: 24 MPVLPQDGEVFVGPGGKKDAQKIGLGLSKLSSKRKDDIQMAKKYAMDISIKQILLRQQKQQQENQQRQQMYSQALSIMSRIYVGSISFEIREDMLRRAFDPFGPIKSINMSWDPATGHHKTFAFVEYEVPEAALLAQESMNGQMLGGRNLKVNSMMFQEMRLPQNMPQAQPIIDMVQKDAKKYFRVYVSSVHPDLSETDLKSVFEAFGEIVKCQLARAPTGRG-HRGFGYLEFNNLTSQSEAIAGMNMFDLGGQYLRVGKCVTPPD 288
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Celegans
Match: rnp-6 (RNP (RRM RNA binding domain) containing [Source:UniProtKB/TrEMBL;Acc:Q9N3S4]) HSP 1 Score: 216.468 bits (550), Expect = 1.245e-61 Identity = 116/266 (43.61%), Postives = 166/266 (62.41%), Query Frame = 1
Query: 94 LPVLEQYGSILEGVGSNKLLEP--FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPN 885
+PVL Q G + G G K + L +L+S KD I AKKYAM+ SI+ +LL+Q KQ + Q +QA+ ++SRIYVGSI++++RE+ +R+A+ FGP+KS+++S+DP T H +AF+E+E PEAA LA + S ++ P NMP + P++D + +A KY R+YV++VHP+LS DL+ VFEAFG I C L T G HR FG++E+ + EAI MN+FDLGGQ LRV K +TPP+
Sbjct: 24 MPVLPQDGEVFVGPGGKKDAQKIGLGLSKLSSKRKDDIQMAKKYAMDISIKQILLRQQKQQQENQQRQQMYSQALSIMSRIYVGSISFEIREDMLRRAFDPFGPIKSINMSWDPATGHHKTFAFVEYEVPEAALLAQESMNGQMLGGRNLKVNSMMFQEMRLPQNMPQAQPIIDMVQKDAKKYFRVYVSSVHPDLSETDLKSVFEAFGEIVKCQLARAPTGRG-HRGFGYLEFNNLTSQSEAIAGMNMFDLGGQYLRVGKCVTPPD 288 HSP 2 Score: 91.2781 bits (225), Expect = 4.560e-19 Identity = 59/145 (40.69%), Postives = 87/145 (60.00%), Query Frame = 1
Query: 1144 MRSAAEIEAMEVEDKSSKTESLENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESRDNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
M A + E M ED S ++ + ++IRG +AR+ +M KL+R V+VLRNM+ DID+ +E E+ EEC K+G V +V I N ++G VKIFV + + ++ A +++GRFFGG V A+ YDQ LFD DY+
Sbjct: 614 MSDALKNEVMNSEDASLASQ----EGLEIRGNDARHLLMTKLMRTNRSNVIVLRNMVTPQDIDEFLEGEIREECGKYGNVIDVVIA-----NFASSGLVKIFVKYSDSMQVDRAKAALDGRFFGGNTVKAEAYDQILFDHADYTG 749
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Celegans
Match: rnp-6 (RNP (RRM RNA binding domain) containing [Source:UniProtKB/TrEMBL;Acc:Q9N3S4]) HSP 1 Score: 201.83 bits (512), Expect = 7.313e-60 Identity = 111/257 (43.19%), Postives = 159/257 (61.87%), Query Frame = 1
Query: 94 LPVLEQYGSILEGVGSNKLLEP--FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLR 858
+PVL Q G + G G K + L +L+S KD I AKKYAM+ SI+ +LL+Q KQ + Q +QA+ ++SRIYVGSI++++RE+ +R+A+ FGP+KS+++S+DP T H +AF+E+E PEAA LA + S ++ P NMP + P++D + +A KY R+YV++VHP+LS DL+ VFEAFG I C L T G HR FG++E+ + EAI MN+FDLGGQ LR
Sbjct: 24 MPVLPQDGEVFVGPGGKKDAQKIGLGLSKLSSKRKDDIQMAKKYAMDISIKQILLRQQKQQQENQQRQQMYSQALSIMSRIYVGSISFEIREDMLRRAFDPFGPIKSINMSWDPATGHHKTFAFVEYEVPEAALLAQESMNGQMLGGRNLKVNSMMFQEMRLPQNMPQAQPIIDMVQKDAKKYFRVYVSSVHPDLSETDLKSVFEAFGEIVKCQLARAPTGRG-HRGFGYLEFNNLTSQSEAIAGMNMFDLGGQYLR 279 HSP 2 Score: 57.3806 bits (137), Expect = 1.206e-8 Identity = 30/75 (40.00%), Postives = 46/75 (61.33%), Query Frame = 1
Query: 637 NRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRV 861
+RIYV ++ + + LR F+ FGPI+S + D G H+ F F+EY+ P+AA+ A +MN LGG+ L+V
Sbjct: 102 SRIYVGSISFEIREDMLRRAFDPFGPIKSINMSWDPA-TGHHKTFAFVEYEVPEAALLAQESMNGQMLGGRNLKV 175
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Celegans
Match: tiar-1 (TIA-1/TIAL RNA binding protein homolog [Source:UniProtKB/TrEMBL;Acc:Q95QV8]) HSP 1 Score: 63.1586 bits (152), Expect = 1.012e-10 Identity = 50/192 (26.04%), Postives = 90/192 (46.88%), Query Frame = 1
Query: 328 IYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPH-SGPLVDDLFAEANKYN-RIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVLP 897
++VG ++ +V + +R+A+ FG V + D T K GY F+ + E A+ A+ Q + + I + K G H + D+++ + + N +YV N+ +L+ +++R F +FG I R K + + F+++ AA +AI MN D+GGQL+R + T P
Sbjct: 34 VFVGDLSSEVDNQKLREAFQPFGDVSDAKVIRDTNTTKSKGYGFVSYPKREEAERAIEQ-MNGQWLGRRTIRTNWATRKPGDQEKPSHYNEKSYDEIYNQTSGDNTSVYVGNI-ASLTEDEIRQGFASFGRITEV-------RIFKMQGYAFVKFDNKDAAAKAIVQMNNQDVGGQLVRCSWGKTGDTGKTP 216
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Celegans
Match: tiar-1 (TIA-1/TIAL RNA binding protein homolog [Source:UniProtKB/TrEMBL;Acc:Q95QV8]) HSP 1 Score: 62.7734 bits (151), Expect = 1.549e-10 Identity = 50/192 (26.04%), Postives = 90/192 (46.88%), Query Frame = 1
Query: 328 IYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPH-SGPLVDDLFAEANKYN-RIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVLP 897
++VG ++ +V + +R+A+ FG V + D T K GY F+ + E A+ A+ Q + + I + K G H + D+++ + + N +YV N+ +L+ +++R F +FG I R K + + F+++ AA +AI MN D+GGQL+R + T P
Sbjct: 24 VFVGDLSSEVDNQKLREAFQPFGDVSDAKVIRDTNTTKSKGYGFVSYPKREEAERAIEQ-MNGQWLGRRTIRTNWATRKPGDQEKPSHYNEKSYDEIYNQTSGDNTSVYVGNI-ASLTEDEIRQGFASFGRITEV-------RIFKMQGYAFVKFDNKDAAAKAIVQMNNQDVGGQLVRCSWGKTGDTGKTP 206
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Fly
Match: hfp (gene:FBgn0028577 transcript:FBtr0072709) HSP 1 Score: 254.603 bits (649), Expect = 1.222e-76 Identity = 127/242 (52.48%), Postives = 172/242 (71.07%), Query Frame = 1
Query: 172 RLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIKRTQ----AMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPN 885
+L+S + D ++KAKKYAMEQSI+ VL+KQT H+ QQ+ + A+ L+ R+YVGSI+++++E+TIR A+ FGP+KS+++S+DPITQKH G+AF+E+E PE AQLAL Q L GR KVGRP+NMP + ++D++ EA +NRIYVA++HP+LS ED++ VFEAFGPI C L T H+ +GFIEY QA EAI +MNLFDLGGQLLRV ++ITPPN
Sbjct: 76 KLSSEQHDLVSKAKKYAMEQSIKMVLMKQTLAHQQQQLATQRTQVQRQQALALMCRVYVGSISFELKEDTIRVAFTPFGPIKSINMSWDPITQKHKGFAFVEYEIPEGAQLALEQMNGALM--------GGRNIKVGRPSNMPQAQQVIDEVQEEAKSFNRIYVASIHPDLSEEDIKSVFEAFGPILYCKLA-QGTSLHTHKGYGFIEYANKQAMDEAIASMNLFDLGGQLLRVGRSITPPN 308 HSP 2 Score: 98.9821 bits (245), Expect = 1.610e-21 Identity = 55/127 (43.31%), Postives = 82/127 (64.57%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIV----LESRDNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
+ +N+ I+G+ AR +MQ+L+RP +V++LRNM+ D+D+ ++EE+ EEC+KFG V V I E+ D+D A VKIFV F + +++GRFFGGR V+A+ YDQ +FD GD S
Sbjct: 511 QQENMSIKGQSARQLVMQRLMRPVDSRVIILRNMVGPEDVDETLQEEIQEECSKFGTVSRVIIFNEKQTENEDDDEAEIIVKIFVEFSAGAEAMRGKEALDGRFFGGRRVVAELYDQGIFDQGDLSG 637
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Fly
Match: hfp (gene:FBgn0028577 transcript:FBtr0300416) HSP 1 Score: 235.728 bits (600), Expect = 2.802e-70 Identity = 120/225 (53.33%), Postives = 161/225 (71.56%), Query Frame = 1
Query: 223 MEQSIRTVLLKQTKQHEVQQM----INIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPN 885
MEQSI+ VL+KQT H+ QQ+ ++R QA+ L+ R+YVGSI+++++E+TIR A+ FGP+KS+++S+DPITQKH G+AF+E+E PE AQLAL Q L GR KVGRP+NMP + ++D++ EA +NRIYVA++HP+LS ED++ VFEAFGPI C L T H+ +GFIEY QA EAI +MNLFDLGGQLLRV ++ITPPN
Sbjct: 1 MEQSIKMVLMKQTLAHQQQQLATQRTQVQRQQALALMCRVYVGSISFELKEDTIRVAFTPFGPIKSINMSWDPITQKHKGFAFVEYEIPEGAQLALEQMNGALM--------GGRNIKVGRPSNMPQAQQVIDEVQEEAKSFNRIYVASIHPDLSEEDIKSVFEAFGPILYCKLAQG-TSLHTHKGYGFIEYANKQAMDEAIASMNLFDLGGQLLRVGRSITPPN 216 HSP 2 Score: 99.3673 bits (246), Expect = 1.017e-21 Identity = 55/127 (43.31%), Postives = 82/127 (64.57%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIV----LESRDNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
+ +N+ I+G+ AR +MQ+L+RP +V++LRNM+ D+D+ ++EE+ EEC+KFG V V I E+ D+D A VKIFV F + +++GRFFGGR V+A+ YDQ +FD GD S
Sbjct: 419 QQENMSIKGQSARQLVMQRLMRPVDSRVIILRNMVGPEDVDETLQEEIQEECSKFGTVSRVIIFNEKQTENEDDDEAEIIVKIFVEFSAGAEAMRGKEALDGRFFGGRRVVAELYDQGIFDQGDLSG 545
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Fly
Match: hfp (gene:FBgn0028577 transcript:FBtr0300418) HSP 1 Score: 235.728 bits (600), Expect = 2.802e-70 Identity = 120/225 (53.33%), Postives = 161/225 (71.56%), Query Frame = 1
Query: 223 MEQSIRTVLLKQTKQHEVQQM----INIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPN 885
MEQSI+ VL+KQT H+ QQ+ ++R QA+ L+ R+YVGSI+++++E+TIR A+ FGP+KS+++S+DPITQKH G+AF+E+E PE AQLAL Q L GR KVGRP+NMP + ++D++ EA +NRIYVA++HP+LS ED++ VFEAFGPI C L T H+ +GFIEY QA EAI +MNLFDLGGQLLRV ++ITPPN
Sbjct: 1 MEQSIKMVLMKQTLAHQQQQLATQRTQVQRQQALALMCRVYVGSISFELKEDTIRVAFTPFGPIKSINMSWDPITQKHKGFAFVEYEIPEGAQLALEQMNGALM--------GGRNIKVGRPSNMPQAQQVIDEVQEEAKSFNRIYVASIHPDLSEEDIKSVFEAFGPILYCKLAQG-TSLHTHKGYGFIEYANKQAMDEAIASMNLFDLGGQLLRVGRSITPPN 216 HSP 2 Score: 99.3673 bits (246), Expect = 1.017e-21 Identity = 55/127 (43.31%), Postives = 82/127 (64.57%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIV----LESRDNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
+ +N+ I+G+ AR +MQ+L+RP +V++LRNM+ D+D+ ++EE+ EEC+KFG V V I E+ D+D A VKIFV F + +++GRFFGGR V+A+ YDQ +FD GD S
Sbjct: 419 QQENMSIKGQSARQLVMQRLMRPVDSRVIILRNMVGPEDVDETLQEEIQEECSKFGTVSRVIIFNEKQTENEDDDEAEIIVKIFVEFSAGAEAMRGKEALDGRFFGGRRVVAELYDQGIFDQGDLSG 545
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Fly
Match: hfp (gene:FBgn0028577 transcript:FBtr0300420) HSP 1 Score: 235.728 bits (600), Expect = 2.802e-70 Identity = 120/225 (53.33%), Postives = 161/225 (71.56%), Query Frame = 1
Query: 223 MEQSIRTVLLKQTKQHEVQQM----INIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPN 885
MEQSI+ VL+KQT H+ QQ+ ++R QA+ L+ R+YVGSI+++++E+TIR A+ FGP+KS+++S+DPITQKH G+AF+E+E PE AQLAL Q L GR KVGRP+NMP + ++D++ EA +NRIYVA++HP+LS ED++ VFEAFGPI C L T H+ +GFIEY QA EAI +MNLFDLGGQLLRV ++ITPPN
Sbjct: 1 MEQSIKMVLMKQTLAHQQQQLATQRTQVQRQQALALMCRVYVGSISFELKEDTIRVAFTPFGPIKSINMSWDPITQKHKGFAFVEYEIPEGAQLALEQMNGALM--------GGRNIKVGRPSNMPQAQQVIDEVQEEAKSFNRIYVASIHPDLSEEDIKSVFEAFGPILYCKLAQG-TSLHTHKGYGFIEYANKQAMDEAIASMNLFDLGGQLLRVGRSITPPN 216 HSP 2 Score: 99.3673 bits (246), Expect = 1.017e-21 Identity = 55/127 (43.31%), Postives = 82/127 (64.57%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIV----LESRDNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
+ +N+ I+G+ AR +MQ+L+RP +V++LRNM+ D+D+ ++EE+ EEC+KFG V V I E+ D+D A VKIFV F + +++GRFFGGR V+A+ YDQ +FD GD S
Sbjct: 419 QQENMSIKGQSARQLVMQRLMRPVDSRVIILRNMVGPEDVDETLQEEIQEECSKFGTVSRVIIFNEKQTENEDDDEAEIIVKIFVEFSAGAEAMRGKEALDGRFFGGRRVVAELYDQGIFDQGDLSG 545
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Fly
Match: hfp (gene:FBgn0028577 transcript:FBtr0072710) HSP 1 Score: 235.728 bits (600), Expect = 2.802e-70 Identity = 120/225 (53.33%), Postives = 161/225 (71.56%), Query Frame = 1
Query: 223 MEQSIRTVLLKQTKQHEVQQM----INIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPN 885
MEQSI+ VL+KQT H+ QQ+ ++R QA+ L+ R+YVGSI+++++E+TIR A+ FGP+KS+++S+DPITQKH G+AF+E+E PE AQLAL Q L GR KVGRP+NMP + ++D++ EA +NRIYVA++HP+LS ED++ VFEAFGPI C L T H+ +GFIEY QA EAI +MNLFDLGGQLLRV ++ITPPN
Sbjct: 1 MEQSIKMVLMKQTLAHQQQQLATQRTQVQRQQALALMCRVYVGSISFELKEDTIRVAFTPFGPIKSINMSWDPITQKHKGFAFVEYEIPEGAQLALEQMNGALM--------GGRNIKVGRPSNMPQAQQVIDEVQEEAKSFNRIYVASIHPDLSEEDIKSVFEAFGPILYCKLAQG-TSLHTHKGYGFIEYANKQAMDEAIASMNLFDLGGQLLRVGRSITPPN 216 HSP 2 Score: 99.3673 bits (246), Expect = 1.017e-21 Identity = 55/127 (43.31%), Postives = 82/127 (64.57%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIV----LESRDNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
+ +N+ I+G+ AR +MQ+L+RP +V++LRNM+ D+D+ ++EE+ EEC+KFG V V I E+ D+D A VKIFV F + +++GRFFGGR V+A+ YDQ +FD GD S
Sbjct: 419 QQENMSIKGQSARQLVMQRLMRPVDSRVIILRNMVGPEDVDETLQEEIQEECSKFGTVSRVIIFNEKQTENEDDDEAEIIVKIFVEFSAGAEAMRGKEALDGRFFGGRRVVAELYDQGIFDQGDLSG 545
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Zebrafish
Match: puf60b (poly-U binding splicing factor b [Source:NCBI gene;Acc:415211]) HSP 1 Score: 263.462 bits (672), Expect = 1.830e-84 Identity = 132/257 (51.36%), Postives = 185/257 (71.98%), Query Frame = 1
Query: 127 EGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK----RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPP 882
+G GS L P L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP ++ + P+++ L EA YNRIYVA++HP+LS +D++ VFEAFG I+SC L + T GKH+ FGFIEY+ PQ++++A+ +MNLFDLGGQ LRV KA+TPP
Sbjct: 6 QGTGSKLGLPP-----LTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPGSIGQAQPIIEQLAEEARAYNRIYVASIHPDLSDDDIKSVFEAFGKIKSCMLAREPTT-GKHKGFGFIEYEKPQSSLDAVSSMNLFDLGGQYLRVGKAVTPP 247
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Zebrafish
Match: puf60b (poly-U binding splicing factor b [Source:NCBI gene;Acc:415211]) HSP 1 Score: 265.003 bits (676), Expect = 8.481e-82 Identity = 135/269 (50.19%), Postives = 190/269 (70.63%), Query Frame = 1
Query: 127 EGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK----RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
+G GS L P L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP ++ + P+++ L EA YNRIYVA++HP+LS +D++ VFEAFG I+SC L + T GKH+ FGFIEY+ PQ++++A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 6 QGTGSKLGLPP-----LTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPGSIGQAQPIIEQLAEEARAYNRIYVASIHPDLSDDDIKSVFEAFGKIKSCMLAREPTT-GKHKGFGFIEYEKPQSSLDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPTTPGGL 259 HSP 2 Score: 74.7146 bits (182), Expect = 8.893e-14 Identity = 47/97 (48.45%), Postives = 63/97 (64.95%), Query Frame = 1
Query: 1291 VMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
VMVLRNM+ DIDD +E EV EEC K+G V V I E + + D+A VKIFV F +N AI ++N R+F GR V+A+ YDQ+ F++ D +A
Sbjct: 404 VMVLRNMVGPEDIDDDLEGEVMEECGKYGAVNRVIIYQERQGEEDDAEIIVKIFVEFSDAGEMNKAIQALNNRWFAGRKVVAELYDQDRFNSSDLTA 500
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Zebrafish
Match: puf60b (poly-U binding splicing factor b [Source:NCBI gene;Acc:415211]) HSP 1 Score: 264.618 bits (675), Expect = 1.256e-81 Identity = 135/269 (50.19%), Postives = 190/269 (70.63%), Query Frame = 1
Query: 127 EGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK----RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
+G GS L P L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP ++ + P+++ L EA YNRIYVA++HP+LS +D++ VFEAFG I+SC L + T GKH+ FGFIEY+ PQ++++A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 5 QGTGSKLGLPP-----LTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPGSIGQAQPIIEQLAEEARAYNRIYVASIHPDLSDDDIKSVFEAFGKIKSCMLAREPTT-GKHKGFGFIEYEKPQSSLDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPTTPGGL 258 HSP 2 Score: 75.0998 bits (183), Expect = 7.832e-14 Identity = 47/97 (48.45%), Postives = 63/97 (64.95%), Query Frame = 1
Query: 1291 VMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
VMVLRNM+ DIDD +E EV EEC K+G V V I E + + D+A VKIFV F +N AI ++N R+F GR V+A+ YDQ+ F++ D +A
Sbjct: 406 VMVLRNMVGPEDIDDDLEGEVMEECGKYGAVNRVIIYQERQGEEDDAEIIVKIFVEFSDAGEMNKAIQALNNRWFAGRKVVAELYDQDRFNSSDLTA 502
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Zebrafish
Match: puf60b (poly-U binding splicing factor b [Source:NCBI gene;Acc:415211]) HSP 1 Score: 264.618 bits (675), Expect = 1.345e-81 Identity = 135/269 (50.19%), Postives = 190/269 (70.63%), Query Frame = 1
Query: 127 EGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK----RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
+G GS L P L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP ++ + P+++ L EA YNRIYVA++HP+LS +D++ VFEAFG I+SC L + T GKH+ FGFIEY+ PQ++++A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 6 QGTGSKLGLPP-----LTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPGSIGQAQPIIEQLAEEARAYNRIYVASIHPDLSDDDIKSVFEAFGKIKSCMLAREPTT-GKHKGFGFIEYEKPQSSLDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPTTPGGL 259 HSP 2 Score: 75.0998 bits (183), Expect = 7.648e-14 Identity = 47/97 (48.45%), Postives = 63/97 (64.95%), Query Frame = 1
Query: 1291 VMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
VMVLRNM+ DIDD +E EV EEC K+G V V I E + + D+A VKIFV F +N AI ++N R+F GR V+A+ YDQ+ F++ D +A
Sbjct: 407 VMVLRNMVGPEDIDDDLEGEVMEECGKYGAVNRVIIYQERQGEEDDAEIIVKIFVEFSDAGEMNKAIQALNNRWFAGRKVVAELYDQDRFNSSDLTA 503
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Zebrafish
Match: puf60a (poly-U binding splicing factor a [Source:ZFIN;Acc:ZDB-GENE-030131-2891]) HSP 1 Score: 251.906 bits (642), Expect = 1.536e-79 Identity = 131/273 (47.99%), Postives = 180/273 (65.93%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK---------------------------RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL 894
L L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA +NRIYVA+VHP+LS +D++ VFEAFG I+SC L D T GKH+ +GFIEY Q+A +A+ +MNLFDLGGQ LRV KA+TPP +L
Sbjct: 24 LGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMPNSLQMASLTMGFGDPLSPLQSVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTLKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHPDLSDDDIKSVFEAFGRIKSCSLARDPTT-GKHKGYGFIEYDKAQSAQDAVSSMNLFDLGGQYLRVGKAVTPPMPLL 286
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Xenopus
Match: puf60 (poly(U) binding splicing factor 60KDa [Source:NCBI gene;Acc:100145091]) HSP 1 Score: 159.073 bits (401), Expect = 6.349e-42 Identity = 105/262 (40.08%), Postives = 157/262 (59.92%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK----RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVG-RPTNMPHSGPLVDDLFAEANKYNRIY---VANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
L L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L + K+G P + ++D K ++ V + P + R+V +G I + L+ + R G F IEY+ Q++ +A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 30 LGLPPLTLEQQEALQKAKKYAMEQSIKSVLVKQTIAHQHQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML-------GGRNIKVKLGLHPCRLALLLCIIDTCAIATRKSCQLCSLPVLHTRP-CHAFIYRIVRGGWGFIGTGGLR--LYRYGGGYGF--IEYEKGQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGL 279 HSP 2 Score: 96.2857 bits (238), Expect = 1.611e-20 Identity = 61/124 (49.19%), Postives = 79/124 (63.71%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
E +++ I G AR+ +MQKL+R VMVLRNM+D DIDD +E EV EEC KFG V V I E + + ++A VKIFV F + AI ++NGR+F GR V+A+ YDQE FD D SA
Sbjct: 397 EQEHMSISGSSARHMVMQKLMRKQESTVMVLRNMVDPRDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSMSSETQKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA 520
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Xenopus
Match: puf60 (poly(U) binding splicing factor 60KDa [Source:NCBI gene;Acc:100145091]) HSP 1 Score: 136.732 bits (343), Expect = 2.020e-34 Identity = 65/125 (52.00%), Postives = 94/125 (75.20%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK----RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNL 519
L L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L
Sbjct: 20 LGLPPLTLEQQEALQKAKKYAMEQSIKSVLVKQTIAHQHQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML 144 HSP 2 Score: 96.2857 bits (238), Expect = 1.136e-20 Identity = 61/124 (49.19%), Postives = 79/124 (63.71%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
E +++ I G AR+ +MQKL+R VMVLRNM+D DIDD +E EV EEC KFG V V I E + + ++A VKIFV F + AI ++NGR+F GR V+A+ YDQE FD D SA
Sbjct: 338 EQEHMSISGSSARHMVMQKLMRKQESTVMVLRNMVDPRDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSMSSETQKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA 461 HSP 3 Score: 64.6994 bits (156), Expect = 1.698e-10 Identity = 44/144 (30.56%), Postives = 62/144 (43.06%), Query Frame = 1
Query: 640 RIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPD-VTRPGKHRAFGFIEYKTPQAAI--------------------------------------------------EAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
R+YV +++ L + +R F FGPI+S + D VT KH+ F F+EY+ P+AA +A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 79 RVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTM--KHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVSISWACTPVGWHFCCVRGGYGFIEYEKGQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGL 220
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Xenopus
Match: ahcyl2 (adenosylhomocysteinase-like 2 [Source:Xenbase;Acc:XB-GENE-948871]) HSP 1 Score: 68.1662 bits (165), Expect = 1.664e-11 Identity = 49/176 (27.84%), Postives = 85/176 (48.30%), Query Frame = 1
Query: 301 TQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMN 828
T A L+ +YVG ++ DV E + + + GP+ S+ + D T++ +GYA++ F+ P A+ AL +T+N + GRP + S D + +++ N+ ++ ++ L F AFG I SC + V R +GF+ ++T +AA AI MN
Sbjct: 4 TGAGYPLASLYVGDLHPDVTEAMLYEKFSPAGPIMSIRVCRDIATRRSLGYAYINFQQPADAERAL--DTMNF------------EVIKGRPIRIMWS---QRDPGLRKSGVGNVFIKNLDESIDNKALYDTFSAFGNILSCKV---VCDEHGSRGYGFVHFETQEAANRAIQTMN 159 HSP 2 Score: 54.6842 bits (130), Expect = 3.097e-7 Identity = 42/184 (22.83%), Postives = 84/184 (45.65%), Query Frame = 1
Query: 319 LSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKA 870
+ +++ +++ + + + + FG + S + D + GY F+ FE EAA A+ T+N + + K R + + A+ ++ +Y+ N ++ + LR +F AFG S + D T G+ R FGF+ Y + A +A+ MN ++ G+++ V +A
Sbjct: 98 VGNVFIKNLDESIDNKALYDTFSAFGNILSCKVVCDEHGSR--GYGFVHFETQEAANRAI--QTMNGM-LLNDRKVFVGHFKSRRERELEYG--------AKVMEFTNVYIKNFGEDMDDKRLREIFSAFGNTLSVKVMMDDT--GRSRGFGFVNYGNHEEAQKAVSEMNGKEVNGRMIYVGRA 266
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Xenopus
Match: ahcyl2 (adenosylhomocysteinase-like 2 [Source:Xenbase;Acc:XB-GENE-948871]) HSP 1 Score: 68.1662 bits (165), Expect = 1.851e-11 Identity = 49/176 (27.84%), Postives = 85/176 (48.30%), Query Frame = 1
Query: 301 TQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMN 828
T A L+ +YVG ++ DV E + + + GP+ S+ + D T++ +GYA++ F+ P A+ AL +T+N + GRP + S D + +++ N+ ++ ++ L F AFG I SC + V R +GF+ ++T +AA AI MN
Sbjct: 4 TGAGYPLASLYVGDLHPDVTEAMLYEKFSPAGPIMSIRVCRDIATRRSLGYAYINFQQPADAERAL--DTMNF------------EVIKGRPIRIMWS---QRDPGLRKSGVGNVFIKNLDESIDNKALYDTFSAFGNILSCKV---VCDEHGSRGYGFVHFETQEAANRAIQTMN 159 HSP 2 Score: 54.6842 bits (130), Expect = 3.238e-7 Identity = 42/181 (23.20%), Postives = 83/181 (45.86%), Query Frame = 1
Query: 328 IYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKA 870
+++ +++ + + + + FG + S + D + GY F+ FE EAA A+ T+N + + K R + + A+ ++ +Y+ N ++ + LR +F AFG S + D T G+ R FGF+ Y + A +A+ MN ++ G+++ V +A
Sbjct: 101 VFIKNLDESIDNKALYDTFSAFGNILSCKVVCDEHGSR--GYGFVHFETQEAANRAI--QTMNGM-LLNDRKVFVGHFKSRRERELEYG--------AKVMEFTNVYIKNFGEDMDDKRLREIFSAFGNTLSVKVMMDDT--GRSRGFGFVNYGNHEEAQKAVSEMNGKEVNGRMIYVGRA 266
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Xenopus
Match: ahcyl2 (adenosylhomocysteinase-like 2 [Source:Xenbase;Acc:XB-GENE-948871]) HSP 1 Score: 68.1662 bits (165), Expect = 1.887e-11 Identity = 49/176 (27.84%), Postives = 85/176 (48.30%), Query Frame = 1
Query: 301 TQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMN 828
T A L+ +YVG ++ DV E + + + GP+ S+ + D T++ +GYA++ F+ P A+ AL +T+N + GRP + S D + +++ N+ ++ ++ L F AFG I SC + V R +GF+ ++T +AA AI MN
Sbjct: 4 TGAGYPLASLYVGDLHPDVTEAMLYEKFSPAGPIMSIRVCRDIATRRSLGYAYINFQQPADAERAL--DTMNF------------EVIKGRPIRIMWS---QRDPGLRKSGVGNVFIKNLDESIDNKALYDTFSAFGNILSCKV---VCDEHGSRGYGFVHFETQEAANRAIQTMN 159 HSP 2 Score: 53.9138 bits (128), Expect = 5.242e-7 Identity = 42/184 (22.83%), Postives = 84/184 (45.65%), Query Frame = 1
Query: 319 LSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKA 870
+ +++ +++ + + + + FG + S + D + GY F+ FE EAA A+ T+N + + K R + + A+ ++ +Y+ N ++ + LR +F AFG S + D T G+ R FGF+ Y + A +A+ MN ++ G+++ V +A
Sbjct: 98 VGNVFIKNLDESIDNKALYDTFSAFGNILSCKVVCDEHGSR--GYGFVHFETQEAANRAI--QTMNGM-LLNDRKVFVGHFKSRRERELEYG--------AKVMEFTNVYIKNFGEDMDDKRLREIFSAFGNTLSVKVMMDDT--GRSRGFGFVNYGNHEEAQKAVSEMNGKEVNGRMIYVGRA 266
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Mouse
Match: Puf60 (poly-U binding splicing factor 60 [Source:MGI Symbol;Acc:MGI:1915209]) HSP 1 Score: 258.84 bits (660), Expect = 6.702e-79 Identity = 131/258 (50.78%), Postives = 183/258 (70.93%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK----RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
L L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA +NRIYVA+VH +LS +D++ VFEAFG I+SC L D T GKH+ +GFIEY+ Q++ +A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 59 LGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTT-GKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGL 306 HSP 2 Score: 99.3673 bits (246), Expect = 1.430e-21 Identity = 63/124 (50.81%), Postives = 80/124 (64.52%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
E +++ I G AR+ +MQKLLR VMVLRNM+D DIDD +E EV EEC KFG V V I E + + ++A VKIFV F + AI ++NGR+FGGR V+A+ YDQE FD D SA
Sbjct: 424 EQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSMASETHKAIQALNGRWFGGRKVVAEVYDQERFDNSDLSA 547
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Mouse
Match: Puf60 (poly-U binding splicing factor 60 [Source:MGI Symbol;Acc:MGI:1915209]) HSP 1 Score: 252.292 bits (643), Expect = 2.602e-76 Identity = 131/275 (47.64%), Postives = 183/275 (66.55%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK---------------------RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
L L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA +NRIYVA+VH +LS +D++ VFEAFG I+SC L D T GKH+ +GFIEY+ Q++ +A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 59 LGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTT-GKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGL 323 HSP 2 Score: 99.3673 bits (246), Expect = 1.418e-21 Identity = 63/124 (50.81%), Postives = 80/124 (64.52%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
E +++ I G AR+ +MQKLLR VMVLRNM+D DIDD +E EV EEC KFG V V I E + + ++A VKIFV F + AI ++NGR+FGGR V+A+ YDQE FD D SA
Sbjct: 441 EQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSMASETHKAIQALNGRWFGGRKVVAEVYDQERFDNSDLSA 564
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Mouse
Match: Pabpc4 (poly(A) binding protein, cytoplasmic 4 [Source:MGI Symbol;Acc:MGI:2385206]) HSP 1 Score: 65.0846 bits (157), Expect = 6.671e-12 Identity = 45/170 (26.47%), Postives = 83/170 (48.82%), Query Frame = 1
Query: 319 LSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMN 828
++ +YVG ++ DV E + + + GPV S+ + D IT++ +GYA++ F+ P A+ AL +T+N G+P + S D + +++ N+ ++ ++ L F AFG I SC + V + + F+ ++T +AA +AI MN
Sbjct: 10 MASLYVGDLHSDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERAL--DTMNF------------DVMKGKPIRIMWSQR---DPSLRKSGVGNVFIKNLDKSIDNKALYDTFSAFGNILSCKV---VCDENGSKGYAFVHFETQEAADKAIEKMN 159
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Mouse
Match: Pabpc1 (poly(A) binding protein, cytoplasmic 1 [Source:MGI Symbol;Acc:MGI:1349722]) HSP 1 Score: 65.855 bits (159), Expect = 8.477e-11 Identity = 46/170 (27.06%), Postives = 83/170 (48.82%), Query Frame = 1
Query: 319 LSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMN 828
++ +YVG ++ DV E + + + GP+ S+ + D IT++ +GYA++ F+ P A+ AL +T+N G+P + S D + I++ N+ ++ ++ L F AFG I SC + V + +GF+ ++T +AA AI MN
Sbjct: 10 MASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERAL--DTMNF------------DVIKGKPVRIMWS---QRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKV---VCDENGSKGYGFVHFETQEAAERAIEKMN 159 HSP 2 Score: 56.9954 bits (136), Expect = 5.074e-8 Identity = 46/181 (25.41%), Postives = 86/181 (47.51%), Query Frame = 1
Query: 328 IYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKA 870
I++ +++ + + + + FG + S + D K GY F+ FE EAA+ A+ + L + R+ VGR + +L A A ++ +Y+ N ++ E L+ +F FGP S + D + GK + FGF+ ++ + A +A+ MN +L G+ + V +A
Sbjct: 101 IFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSK--GYGFVHFETQEAAERAIEKMNGMLL--------NDRKVFVGRFKSRKER---EAELGARAKEFTNVYIKNFGEDMDDERLKELFGKFGPALSVKVMTDES--GKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRA 266 HSP 3 Score: 56.225 bits (134), Expect = 8.830e-8 Identity = 47/177 (26.55%), Postives = 80/177 (45.20%), Query Frame = 1
Query: 319 LSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ------NTVNLYPXXXXXXXXXXXXKVGRPTNMPHS-GPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMN 828
+ +Y+ + D+ +E +++ + FGP SV + D + K G+ F+ FE E AQ A+ + N +Y + Q KV R T + + D N +YV N+ + E LR F FG I S + + G+ + FGF+ + +P+ A +A+ MN
Sbjct: 190 FTNVYIKNFGEDMDDERLKELFGKFGPALSVKVMTDE-SGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIY-------VGRAQKKVERQTELKRKFEQMKQDRITRYQGVN-LYVKNLDDGIDDERLRKEFSPFGTITSAKV---MMEGGRSKGFGFVCFSSPEEATKAVTEMN 354
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Mouse
Match: Pabpc5 (poly(A) binding protein, cytoplasmic 5 [Source:MGI Symbol;Acc:MGI:2136401]) HSP 1 Score: 64.6994 bits (156), Expect = 1.068e-10 Identity = 48/182 (26.37%), Postives = 86/182 (47.25%), Query Frame = 1
Query: 334 VGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITP 879
VG ++ DV E+ + + + GP++ + DP+T+ +GY ++ F +P A+ AL NT+N I+ G+P + S P DD ++ N I++ N+ + + L +F AFG I SC + V + + ++ + + AA AI MN L + + V + P
Sbjct: 21 VGDLDPDVTEDMLYKKFRPAGPLRFTRICRDPVTRSPLGYGYVNFRFPADAEWAL--NTMNFDLIN------------GKPFRLMWSQP--DDRLRKSGVGN-IFIKNLDKTIDNRALFYLFSAFGNILSCKV---VCDDNGSKGYAYVHFDSLAAANRAIWHMNGVRLNNRQVYVGRFKFP 182 HSP 2 Score: 51.9878 bits (123), Expect = 1.053e-6 Identity = 48/185 (25.95%), Postives = 84/185 (45.41%), Query Frame = 1
Query: 319 LSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKA 870
+ I++ +++ + + + FG + S + D K GYA++ F+ AA A+ N V L + RQ VGR V E + ++V N ++ E L +F +GP ES + D T GK + FGF+ Y+T +AA +A+ ++ + G++L V +A
Sbjct: 104 VGNIFIKNLDKTIDNRALFYLFSAFGNILSCKVVCDDNGSK--GYAYVHFDSLAAANRAIWHMNGVRL---------NNRQVYVGRFKFPEERAAEVRT--RERATFTNVFVKNFGDDIDDEKLNKLFSEYGPTESVKVIRDAT--GKSKGFGFVRYETHEAAQKAVLELHGKSIDGKVLCVGRA 273
BLAST of Poly(U)-binding-splicing factor PUF60 vs. UniProt/SwissProt
Match: sp|Q6IQE0|PU60B_DANRE (Poly(U)-binding-splicing factor PUF60-B OS=Danio rerio OX=7955 GN=puf60b PE=2 SV=2) HSP 1 Score: 258.455 bits (659), Expect = 2.582e-78 Identity = 135/286 (47.20%), Postives = 190/286 (66.43%), Query Frame = 1
Query: 127 EGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK---------------------RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
+G GS L P L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP ++ + P+++ L EA YNRIYVA++HP+LS +D++ VFEAFG I+SC L + T GKH+ FGFIEY+ PQ++++A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 5 QGTGSKLGLPP-----LTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSSLQSVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPGSIGQAQPIIEQLAEEARAYNRIYVASIHPDLSDDDIKSVFEAFGKIKSCMLAREPTT-GKHKGFGFIEYEKPQSSLDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPTTPGGL 275 HSP 2 Score: 74.7146 bits (182), Expect = 7.183e-13 Identity = 47/97 (48.45%), Postives = 63/97 (64.95%), Query Frame = 1
Query: 1291 VMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
VMVLRNM+ DIDD +E EV EEC K+G V V I E + + D+A VKIFV F +N AI ++N R+F GR V+A+ YDQ+ F++ D +A
Sbjct: 420 VMVLRNMVGPEDIDDDLEGEVMEECGKYGAVNRVIIYQERQGEEDDAEIIVKIFVEFSDAGEMNKAIQALNNRWFAGRKVVAELYDQDRFNSSDLTA 516
BLAST of Poly(U)-binding-splicing factor PUF60 vs. UniProt/SwissProt
Match: sp|Q2HJG2|PUF60_BOVIN (Poly(U)-binding-splicing factor PUF60 OS=Bos taurus OX=9913 GN=PUF60 PE=2 SV=1) HSP 1 Score: 252.292 bits (643), Expect = 8.820e-76 Identity = 131/275 (47.64%), Postives = 183/275 (66.55%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK---------------------RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
L L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA +NRIYVA+VH +LS +D++ VFEAFG I+SC L D T GKH+ +GFIEY+ Q++ +A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 25 LGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTT-GKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGL 289 HSP 2 Score: 96.2857 bits (238), Expect = 8.361e-20 Identity = 62/124 (50.00%), Postives = 78/124 (62.90%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
E +++ I G AR+ +MQKLLR VMVLRNM+D DIDD +E EV EEC KFG V V I E + + ++A VKIFV F + AI +NGR+F GR V+A+ YDQE FD D SA
Sbjct: 407 EQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSVASETHKAIQDLNGRWFAGRKVVAEVYDQERFDNSDLSA 530
BLAST of Poly(U)-binding-splicing factor PUF60 vs. UniProt/SwissProt
Match: sp|Q8T6B9|PUF68_DROME (Poly(U)-binding-splicing factor half pint OS=Drosophila melanogaster OX=7227 GN=hfp PE=1 SV=2) HSP 1 Score: 254.603 bits (649), Expect = 1.225e-75 Identity = 127/242 (52.48%), Postives = 172/242 (71.07%), Query Frame = 1
Query: 172 RLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIKRTQ----AMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPN 885
+L+S + D ++KAKKYAMEQSI+ VL+KQT H+ QQ+ + A+ L+ R+YVGSI+++++E+TIR A+ FGP+KS+++S+DPITQKH G+AF+E+E PE AQLAL Q L GR KVGRP+NMP + ++D++ EA +NRIYVA++HP+LS ED++ VFEAFGPI C L T H+ +GFIEY QA EAI +MNLFDLGGQLLRV ++ITPPN
Sbjct: 76 KLSSEQHDLVSKAKKYAMEQSIKMVLMKQTLAHQQQQLATQRTQVQRQQALALMCRVYVGSISFELKEDTIRVAFTPFGPIKSINMSWDPITQKHKGFAFVEYEIPEGAQLALEQMNGALM--------GGRNIKVGRPSNMPQAQQVIDEVQEEAKSFNRIYVASIHPDLSEEDIKSVFEAFGPILYCKLA-QGTSLHTHKGYGFIEYANKQAMDEAIASMNLFDLGGQLLRVGRSITPPN 308 HSP 2 Score: 98.9821 bits (245), Expect = 1.614e-20 Identity = 55/127 (43.31%), Postives = 82/127 (64.57%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIV----LESRDNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
+ +N+ I+G+ AR +MQ+L+RP +V++LRNM+ D+D+ ++EE+ EEC+KFG V V I E+ D+D A VKIFV F + +++GRFFGGR V+A+ YDQ +FD GD S
Sbjct: 511 QQENMSIKGQSARQLVMQRLMRPVDSRVIILRNMVGPEDVDETLQEEIQEECSKFGTVSRVIIFNEKQTENEDDDEAEIIVKIFVEFSAGAEAMRGKEALDGRFFGGRRVVAELYDQGIFDQGDLSG 637
BLAST of Poly(U)-binding-splicing factor PUF60 vs. UniProt/SwissProt
Match: sp|Q9UHX1|PUF60_HUMAN (Poly(U)-binding-splicing factor PUF60 OS=Homo sapiens OX=9606 GN=PUF60 PE=1 SV=1) HSP 1 Score: 252.677 bits (644), Expect = 1.324e-75 Identity = 131/275 (47.64%), Postives = 183/275 (66.55%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK---------------------RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
L L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA +NRIYVA+VH +LS +D++ VFEAFG I+SC L D T GKH+ +GFIEY+ Q++ +A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 54 LGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTT-GKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGL 318 HSP 2 Score: 96.6709 bits (239), Expect = 7.445e-20 Identity = 62/124 (50.00%), Postives = 79/124 (63.71%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
E +++ I G AR+ +MQKLLR VMVLRNM+D DIDD +E EV EEC KFG V V I E + + ++A VKIFV F + AI ++NGR+F GR V+A+ YDQE FD D SA
Sbjct: 436 EQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA 559
BLAST of Poly(U)-binding-splicing factor PUF60 vs. UniProt/SwissProt
Match: sp|Q5R469|PUF60_PONAB (Poly(U)-binding-splicing factor PUF60 OS=Pongo abelii OX=9601 GN=PUF60 PE=2 SV=1) HSP 1 Score: 252.292 bits (643), Expect = 1.718e-75 Identity = 131/275 (47.64%), Postives = 183/275 (66.55%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK---------------------RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
L L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA +NRIYVA+VH +LS +D++ VFEAFG I+SC L D T GKH+ +GFIEY+ Q++ +A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 53 LGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTT-GKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGL 317 HSP 2 Score: 96.6709 bits (239), Expect = 7.420e-20 Identity = 62/124 (50.00%), Postives = 79/124 (63.71%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
E +++ I G AR+ +MQKLLR VMVLRNM+D DIDD +E EV EEC KFG V V I E + + ++A VKIFV F + AI ++NGR+F GR V+A+ YDQE FD D SA
Sbjct: 435 EQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA 558
BLAST of Poly(U)-binding-splicing factor PUF60 vs. TrEMBL
Match: A0A448WN48 (Uncharacterized protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS9267 PE=4 SV=1) HSP 1 Score: 337.806 bits (865), Expect = 2.917e-106 Identity = 216/513 (42.11%), Postives = 308/513 (60.04%), Query Frame = 1
Query: 100 VLEQYGSILEGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQM---------------INIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVLPGSMXXXXXXXXXXXXXXXXXXXXHTLEVEQVVKVEEAGTXXXXXXXXXXXXXXXXXXXXVLPPTMIIPDCSSMSS----QINEKVAMRSAAEIEAMEVEDKSSKTESLENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESRDNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
V EQ G + G GS +PF L + + +++AI++AKK+A+EQS+++ KQ +Q + Q+ IN K+ Q + LLSRIYVGSI+YD++E+ IRQA+ FGP++SVSLS+D +TQKH G+AF+E+E PE+AQLA+ Q N +L GRQ KVGRP+N+P + P+V++L E N RIYV+ VH +LS EDL LVFEAFG I SC L PD T+ KHR FG+IEY+T QAA +A+ +MNLFDLGGQLLRV KA++PP + S + A A A+ +A + + +K G A L ++ IP SS + Q+ + + S E+ + +D E E N IRGR+AR+ +MQKL+RP V++LRNM+ D DD +E EV EEC K+G+V V I E ++N + +KIFVVF ++S++ A++S++GR+F GR V A+ YD + FDA D +
Sbjct: 43 VYEQNGPVYNGPGSRVENQPFMLIKPDIQQREAIDRAKKFAIEQSVKSAWEKQARQQQQLQVQHQQQQQQQLLQAPAIN-KKQQTLALLSRIYVGSISYDLKEDAIRQAFVPFGPIRSVSLSWDQMTQKHKGFAFIEYENPESAQLAVEQMNDSSL---------GGRQLKVGRPSNLPRAEPMVEELVREHNLEKRIYVSGVHADLSDEDLGLVFEAFGKIISCKLAPDPTKAQKHRGFGYIEYETEQAANDAVASMNLFDLGGQLLRVGKAMSPPEGAISTS---NMSNVPVAAAVAAASATAKVMALGNNIKARSEGLMAGN-----------------LMASLGIPGISSNDTSSWDQVEAQEVLNSNNELASTSFDDDGGTLEHQE--NFMIRGRDARSAIMQKLIRPPTQGVLILRNMVGPEDCDDDLEGEVKEECCKYGQVDRVVIYQEL--DENGSLIIKIFVVFSESESVDRAVSSLHGRYFAGRQVSAESYDLDKFDANDLTG 521
BLAST of Poly(U)-binding-splicing factor PUF60 vs. TrEMBL
Match: A0A4S2L9T6 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008914 PE=4 SV=1) HSP 1 Score: 334.724 bits (857), Expect = 2.759e-105 Identity = 214/494 (43.32%), Postives = 306/494 (61.94%), Query Frame = 1
Query: 106 EQYGSILEGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVLPGSMXXXXXXXXXXXXXXXXXXXXHTLEVEQVVKVEEAGTXXXXXXXXXXXXXXXXXXXXVLPPTMIIPDCSSMSSQINEKVAMRSAAEIEAMEVEDKSSKTESLENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESRDNDNANGE--VKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
+Q G + G GS +PF L +L +++AI +AKKYAME+SI++ L KQT+Q ++QQ K+ Q + LLSRIYVGSI+YD++E++I+ A+ FGP+++V++S+D TQKH G+AF+EFE+PEAA LA+ Q N +L GRQ KVGRP+N+P + PL+++L E ++YVA VHP+LS +DL LVFEAFG I SC L D T+ KHR FG+IEY++ QAA +A+ +MN+FDLGGQ LRV KAI PP + + S AA A AAAS TA+V LE E K G+ + + P + S +++ + A+ +D + E + +N IRGR+ARN +MQKL+RP +++LRNM+ +D DD++E EV EEC +G V V I E + NGE +KIFVVF+ ++S++ AI S+NGR+F GR V+A+ YD E F+A D +
Sbjct: 49 DQAGPVYNGPGSLLTRQPFMLMKLELEQREAIERAKKYAMEESIKSALAKQTRQLQLQQTPATKKAQTLNLLSRIYVGSISYDLKEDSIKTAFQPFGPIRTVTMSWDAATQKHKGFAFIEFEFPEAAHLAVEQMNNASL---------GGRQLKVGRPSNLPQADPLINELVTEYKLEKKVYVAGVHPDLSEDDLALVFEAFGKITSCKLHVDPTKAQKHRGFGYIEYESEQAANDAVASMNMFDLGGQFLRVGKAIAPPEDPPTPTNSSLSSAAAAAAAAASATAKVMALEAESASKGRSPGSNTFANFPIQS------------------PAGGDLGSLSWDRIEAQEASVGAGFSEDDDGATLE--QQENFHIRGRDARNVIMQKLIRPPPRGILILRNMVGPDDCDDELENEVKEECCNYGSVDRVIIYQEL----DENGELIIKIFVVFQDSESVDRAIASLNGRYFAGRQVVAEPYDLEKFEANDLTG 509
BLAST of Poly(U)-binding-splicing factor PUF60 vs. TrEMBL
Match: A0A4W5P4V6 (Poly(U) binding splicing factor 60 OS=Hucho hucho OX=62062 GN=PUF60 (1 of many) PE=4 SV=1) HSP 1 Score: 325.094 bits (832), Expect = 2.490e-101 Identity = 212/517 (41.01%), Postives = 301/517 (58.22%), Query Frame = 1
Query: 127 EGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK---------------------RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSMXXXXXXXXXXXXXXXXXXXXHTLEVEQVVKVEEAGTXXXXXXXXXXXXXXXXXXXXVLP------PTMIIPDCSSMSSQINEKVAMRSAAEIEAMEVEDKSSKTESL-ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYS 1575
+G G+ L P L +++A+ +AKKYAMEQSI++ L+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP N+ + P++D L EA YNRIYVA+VHP+LS ED++ VFEAFG I+SC L D T G+H++FGF+EY PQ++++A+ +MNLFDLGGQ LRV KA+TPP +L PG + ++ AA A A + T ++ A + + + VLP P + P + +Q N + + E E+ + E L + +++ I G AR+ +MQKLLR + VMVLRNM+ DIDD +E EV EEC KFG V V I E + + ++A VKIFV F +N AI ++N R+FGGR VIA+ YDQE F++ D S
Sbjct: 20 QGTGAKLGLPP-----LTPEQQEALQRAKKYAMEQSIKSALVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEIPEAAQLALEQMNSVML---------GGRNIKVGRPGNIGQAQPIIDQLAEEARAYNRIYVASVHPDLSDEDIKSVFEAFGRIKSCTLARDPTT-GRHKSFGFVEYDKPQSSLDAVSSMNLFDLGGQYLRVGKAVTPPMPMLTPTQPGGLPPAAAVAAAAATAKITAQEAVT--GASILGAMAGAVMAAQAPGMITGVTPVRPNLPVLPQVGLVNPVLASPPVITNPAQPNPSLQELQKKKQEEKEMLQDGTGQEMLSDQEHMSISGSSARHMVMQKLLRKSESTVMVLRNMVGPEDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSMASEMNKAIQALNDRWFGGRKVIAEVYDQERFNSSDLS 519
BLAST of Poly(U)-binding-splicing factor PUF60 vs. TrEMBL
Match: A0A3B4CRZ1 (Poly-U binding splicing factor b OS=Pygocentrus nattereri OX=42514 PE=4 SV=1) HSP 1 Score: 320.472 bits (820), Expect = 1.915e-99 Identity = 209/503 (41.55%), Postives = 306/503 (60.83%), Query Frame = 1
Query: 127 EGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK----RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSMXXXXXXXXXXXXXXXXXXXXHTLEVEQVVKVEEAGTXXXXXXXXXXXXXXXXXXXXVLP--PTM-IIPDCSSMSSQI-NEKVAMRSAAEIEAMEVEDKSSKTESLENDNVKIRGREARNQMMQKLLRPTVHK----VMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESRDNDNANGE--VKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
+G+GS L P L +++A+ +AKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA YNRIYV++VHP+LS ED++ VFEAFG I+SC L + T GKH+ + FIEY+ PQ+AI+A+ +MNLFDLGGQ LRV KA+TPP +L PG + ++ AA A A E +V + ++ + + V P PT+ ++P ++ + + + + + E+E + E+ +++ + L + R E R Q+ + R TV + VMVLRNM+ DIDD +E EV EEC KFG V V I+ + R + + E VKIFV F T+ +N AI ++N R+F GR V+A+ YDQE F+ D SA
Sbjct: 64 QGMGSKLALPP-----LTPEQQEALQRAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAYNRIYVSSVHPDLSDEDIKSVFEAFGKIKSCMLAREPTT-GKHKGYCFIEYEKPQSAIDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPTTPGGLPPAAAVAAAAATAKITAQ-------EALVSPAQILSQQIGLPQAVLAAQAPGLITGVTPVRPTLPVVPQVGLVNPVLASPPLPVETKKEVEEKQREENATRADGLGPE----RASE-REQLGSSVGRHTVAQSGSTVMVLRNMVGPEDIDDDLEGEVTEECGKFGAVNRV-IIYQERQGEEEDAEIIVKIFVEFSDTEEMNKAIQALNNRWFAGRKVVAEVYDQERFNNSDLSA 538
BLAST of Poly(U)-binding-splicing factor PUF60 vs. TrEMBL
Match: A0A1A8EU20 (Poly-U binding splicing factor a OS=Nothobranchius korthausae OX=1143690 GN=PUF60A PE=4 SV=1) HSP 1 Score: 319.316 bits (817), Expect = 4.224e-99 Identity = 203/504 (40.28%), Postives = 296/504 (58.73%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQ--------------------QMINIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSMXXXXXXXXXXXXXXXXXXXXHTLEVEQVVKVEEAGTXXXXXXXXXXXXXXXXXXXXVLPPTMIIPDCSSMSSQINEKVAMRSAAEIEAMEVEDKSSKTESL----ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESRDNDNANGE--VKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
L L +++DA+ KAKKYAMEQSI++VL+KQT + Q Q + +R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P+++ L EA +NRIYVA+VH +LS ED++ VFEAFG I+SC L + T G+H+ +GFIEY PQ+A++A+ +MNLFDLGGQ LRV KA+TPP +L PG + ++ AA A +A + V + ++ + V V P +P ++ + R++ E E++ + E++ E ++V I G AR+ +M+KLLR VMVLRNM+ DIDD +E EV EEC KFG+V+ V I+ + R + + E VKIFV F +N AI ++N R+FGGR V+A+ Y+QE F++ D SA
Sbjct: 29 LGLPPLTPNQQDALQKAKKYAMEQSIKSVLVKQTLVQQQQLASLQMAPLAMGLGDALSPLQSVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFIPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIEQLAEEARAFNRIYVASVHADLSDEDIKSVFEAFGRIKSCMLSREPTT-GRHKGYGFIEYDKPQSALDAVASMNLFDLGGQYLRVGKAVTPPVALLTPMTPGGLPAAAAVAAAAASAKITAQEAVGASVLGALAGPALLSQQLGLPQAVMAAQAPGVITGVTPIRASLPHVGLVNPVLATPPYARTSVSSEQKNTEEEVQQDETMEPLSEQEHVSISGNSARHMVMRKLLRKQESIVMVLRNMVGPEDIDDDLEGEVMEECGKFGQVKRV-IIYQERQGEEEDAEIVVKIFVEFSEVAEMNRAIQALNNRWFGGRKVVAEVYEQERFNSNDLSA 521
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Cavefish
Match: puf60b (poly(U)-binding-splicing factor PUF60-B-like [Source:NCBI gene;Acc:103037989]) HSP 1 Score: 263.462 bits (672), Expect = 2.226e-81 Identity = 136/269 (50.56%), Postives = 189/269 (70.26%), Query Frame = 1
Query: 127 EGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK----RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
+G+GS L P L +++A+ +AKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP N+ + P++D L EA YNRIYVA+VHP+LS +D++ VFEAFG I+SC L + T GKH+ + FIEY PQ+A++A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 5 QGMGSKLGLPP-----LTPEQQEALQRAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPGNIGQAQPIIDQLAEEARAYNRIYVASVHPDLSDDDIKSVFEAFGKIKSCMLAREPTT-GKHKGYCFIEYDKPQSAVDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPTTPGGL 258 HSP 2 Score: 76.6406 bits (187), Expect = 1.808e-14 Identity = 49/97 (50.52%), Postives = 64/97 (65.98%), Query Frame = 1
Query: 1291 VMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
VMVLRNM+ DIDD +E EV EEC KFG V V I E + + D+A VKIFV F ++ +N AI ++N R+F GR V+A+ YDQ+ F+ D SA
Sbjct: 399 VMVLRNMVGPEDIDDDLEGEVTEECGKFGAVNRVIIYQERQGEEDDAEIIVKIFVEFSDSEEMNKAIQALNNRWFAGRKVVAEVYDQQRFNNSDLSA 495
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Cavefish
Match: puf60b (poly(U)-binding-splicing factor PUF60-B-like [Source:NCBI gene;Acc:103037989]) HSP 1 Score: 256.914 bits (655), Expect = 9.413e-79 Identity = 136/286 (47.55%), Postives = 189/286 (66.08%), Query Frame = 1
Query: 127 EGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK---------------------RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
+G+GS L P L +++A+ +AKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP N+ + P++D L EA YNRIYVA+VHP+LS +D++ VFEAFG I+SC L + T GKH+ + FIEY PQ+A++A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 5 QGMGSKLGLPP-----LTPEQQEALQRAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPGNIGQAQPIIDQLAEEARAYNRIYVASVHPDLSDDDIKSVFEAFGKIKSCMLAREPTT-GKHKGYCFIEYDKPQSAVDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPTTPGGL 275 HSP 2 Score: 76.6406 bits (187), Expect = 1.897e-14 Identity = 49/97 (50.52%), Postives = 64/97 (65.98%), Query Frame = 1
Query: 1291 VMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
VMVLRNM+ DIDD +E EV EEC KFG V V I E + + D+A VKIFV F ++ +N AI ++N R+F GR V+A+ YDQ+ F+ D SA
Sbjct: 416 VMVLRNMVGPEDIDDDLEGEVTEECGKFGAVNRVIIYQERQGEEDDAEIIVKIFVEFSDSEEMNKAIQALNNRWFAGRKVVAEVYDQQRFNNSDLSA 512
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Cavefish
Match: puf60a (poly(U)-binding-splicing factor PUF60 [Source:NCBI gene;Acc:103042138]) HSP 1 Score: 253.832 bits (647), Expect = 2.067e-77 Identity = 130/247 (52.63%), Postives = 176/247 (71.26%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQM-----INIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPP 882
L L +++A+ KAKKYAMEQSI++VL+KQT H+ QQ + +R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA +NRIYVA+VHP+LS ED++ VFEAFG I+SC L D T GKH+ +GFIEY Q+A +A+ +MNLFDLGGQ LRV KA+TPP
Sbjct: 28 LGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTLAHQQQQQLNNLQVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTLKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHPDLSDEDIKSVFEAFGRIKSCTLARDPTT-GKHKGYGFIEYDKAQSAQDAVSSMNLFDLGGQYLRVGKAVTPP 264 HSP 2 Score: 100.523 bits (249), Expect = 4.242e-22 Identity = 63/124 (50.81%), Postives = 80/124 (64.52%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
E +++ I G AR+ +MQKLLR VMVLRNM+ DIDD +E EV EEC KFG V V I E + + ++A VKIFV F + +N AI ++N R+FGGR VIA+ YDQE FD D SA
Sbjct: 392 EQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVGPEDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEVIVKIFVEFSAASEMNKAIQALNNRWFGGRKVIAEVYDQERFDNSDLSA 515
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Cavefish
Match: puf60a (poly(U)-binding-splicing factor PUF60 [Source:NCBI gene;Acc:103042138]) HSP 1 Score: 246.128 bits (627), Expect = 2.503e-74 Identity = 130/271 (47.97%), Postives = 176/271 (64.94%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQ-----------------------------QMINIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPP 882
L L +++A+ KAKKYAMEQSI++VL+KQT H+ Q Q + +R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA +NRIYVA+VHP+LS ED++ VFEAFG I+SC L D T GKH+ +GFIEY Q+A +A+ +MNLFDLGGQ LRV KA+TPP
Sbjct: 11 LGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTLAHQQQQQLNNLQLPNSLQMASLTMAGFGDPLSPLQSVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTLKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHPDLSDEDIKSVFEAFGRIKSCTLARDPTT-GKHKGYGFIEYDKAQSAQDAVSSMNLFDLGGQYLRVGKAVTPP 271 HSP 2 Score: 100.523 bits (249), Expect = 4.259e-22 Identity = 63/124 (50.81%), Postives = 80/124 (64.52%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
E +++ I G AR+ +MQKLLR VMVLRNM+ DIDD +E EV EEC KFG V V I E + + ++A VKIFV F + +N AI ++N R+FGGR VIA+ YDQE FD D SA
Sbjct: 418 EQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVGPEDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEVIVKIFVEFSAASEMNKAIQALNNRWFGGRKVIAEVYDQERFDNSDLSA 541
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Cavefish
Match: puf60a (poly(U)-binding-splicing factor PUF60 [Source:NCBI gene;Acc:103042138]) HSP 1 Score: 246.514 bits (628), Expect = 2.876e-74 Identity = 135/309 (43.69%), Postives = 185/309 (59.87%), Query Frame = 1
Query: 61 FVFLTKIIMHFLPVLEQYGSILEGVG-----SNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQ-----------------------------QMINIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPP 882
F +++ VL G + G L L +++A+ KAKKYAMEQSI++VL+KQT H+ Q Q + +R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA +NRIYVA+VHP+LS ED++ VFEAFG I+SC L D T GKH+ +GFIEY Q+A +A+ +MNLFDLGGQ LRV KA+TPP
Sbjct: 5 FTVFIAVVLALEGVLHGVGDFVRGEALTMENGQSTASKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTLAHQQQQQLNNLQLPNSLQMASLTMAGFGDPLSPLQSVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTLKHKGFAFVEYEVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHPDLSDEDIKSVFEAFGRIKSCTLARDPTT-GKHKGYGFIEYDKAQSAQDAVSSMNLFDLGGQYLRVGKAVTPP 303 HSP 2 Score: 100.138 bits (248), Expect = 5.572e-22 Identity = 63/124 (50.81%), Postives = 80/124 (64.52%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
E +++ I G AR+ +MQKLLR VMVLRNM+ DIDD +E EV EEC KFG V V I E + + ++A VKIFV F + +N AI ++N R+FGGR VIA+ YDQE FD D SA
Sbjct: 431 EQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVGPEDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEVIVKIFVEFSAASEMNKAIQALNNRWFGGRKVIAEVYDQERFDNSDLSA 554
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010908.1 (pep scaffold:Pmarinus_7.0:GL483688:3845:10066:1 gene:ENSPMAG00000009884.1 transcript:ENSPMAT00000010908.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 166.007 bits (419), Expect = 4.237e-49 Identity = 81/163 (49.69%), Postives = 115/163 (70.55%), Query Frame = 1
Query: 295 KRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFI 780
+R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+DP+T KH G+AF+E++ PEAAQLA+ Q N + L GR KVGRP+N+ + P++D L EA +NRIYVA+VHP+LS +D++ VFEAFG I C L D GKH+ +GFI
Sbjct: 10 QRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSFDPVTMKHKGFAFVEYDLPEAAQLAMEQMNGITL---------GGRSIKVGRPSNIGQAQPIIDQLAEEAKTHNRIYVASVHPDLSDDDIKSVFEAFGKITMCTLARDPVL-GKHKGYGFI 162 HSP 2 Score: 56.6102 bits (135), Expect = 8.404e-10 Identity = 30/77 (38.96%), Postives = 46/77 (59.74%), Query Frame = 1
Query: 640 RIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPD-VTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAK 867
R+YV +++ L + +R F FGPI+S + D VT KH+ F F+EY P+AA A+ MN LGG+ ++V +
Sbjct: 20 RVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSFDPVTM--KHKGFAFVEYDLPEAAQLAMEQMNGITLGGRSIKVGR 94
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Sea Lamprey
Match: pabpc4 (poly(A) binding protein, cytoplasmic 4 (inducible form) [Source:ZFIN;Acc:ZDB-GENE-030131-9663]) HSP 1 Score: 67.781 bits (164), Expect = 2.601e-12 Identity = 49/180 (27.22%), Postives = 87/180 (48.33%), Query Frame = 1
Query: 319 LSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLR 858
++ +YVG ++ DV E + + + GPV S+ + D IT++ +GYA++ F+ P A+ AL +T+N G+P + S D + +++ N+ ++ ++ + F AFG I SC + V R +GF+ ++T +AA AI MN L +L R
Sbjct: 10 MASLYVGDLHPDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERAL--DTMNF------------DVIKGKPIRIMWS---QRDPSLRKSGVGNVFIKNLDKSIDNKAMYDTFSAFGNILSCKV---VCDENGSRGYGFVHFETQEAADRAIEKMNGMLLNDRLAR 169 HSP 2 Score: 57.3806 bits (137), Expect = 5.023e-9 Identity = 50/185 (27.03%), Postives = 87/185 (47.03%), Query Frame = 1
Query: 319 LSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVG-RPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKA 870
+ +Y+ + D+ E+ +R+ + FG SV + D K G+ F+ FE E AQ A+ N +N ++ GR K G R + ++ + +YV N+ N+ E LR F +G I S + ++ G+ + FGF+ + +P+ A +A+ MN +G + L VA A
Sbjct: 193 FTNVYIKNFGDDMDEDKLREFFGKFGKALSVCVMSDECG-KSRGFGFVSFEQHEDAQRAV--NEMNGKELNGRTVFVGRAQKKGERQAELKRKFEMLKQERISRYQGVNLYVKNLDDNIDDEQLRKEFAPYGTITSTKV---MSEEGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALA 371
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Sea Lamprey
Match: RBM39 (RNA binding motif protein 39 [Source:HGNC Symbol;Acc:HGNC:15923]) HSP 1 Score: 58.9214 bits (141), Expect = 1.444e-9 Identity = 42/177 (23.73%), Postives = 83/177 (46.89%), Query Frame = 1
Query: 355 VREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNT-----VNLYPXXXXXXXXXXXXKVGRPTNMPHS--GPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVA 864
+R + + + G V+ V + D +++ G A++EF ++ LA+ V + + + + M S GP+ R+YV ++H N++ + L+ +FE FG IE+C L D + G+ R +GF+ + + A +A+ +N F+L G+ ++V
Sbjct: 170 IRPRDLEEFFSAVGKVRDVRMISDRNSRRSKGIAYIEFVEVDSVSLAIGLTGQRLLGVPIIVQASQAEKNRAAAAAAAAATMQKSTAGPM------------RLYVGSLHFNITEDMLKGIFEPFGKIENCQLMMD-SDTGRSRGYGFVTFADAECARKALEQLNGFELAGRPMKVG 333
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001053.1 (pep scaffold:Pmarinus_7.0:GL478206:26457:39057:1 gene:ENSPMAG00000000940.1 transcript:ENSPMAT00000001053.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 57.3806 bits (137), Expect = 4.397e-9 Identity = 48/139 (34.53%), Postives = 77/139 (55.40%), Query Frame = 1
Query: 1168 AMEVEDKSSKT-ESLENDNVKIRG--------REARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESRDNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELF 1557
A+ VE S + ++L DNV G ++ N + + L PT KV++LRNM+ ++D+ +E E EEC K+G+V +K V+ + V+IF+ F+ +S A+ +NGR+FGGRIV A Y+ + F
Sbjct: 265 ALSVEKTSKRGGKTLIGDNVPKEGLHANTSAKKQEPNPLTEILKNPT--KVVLLRNMVGPGEVDEDLESETKEECEKYGKV--IKCVIFEMPGAPEDDAVRIFLEFERMESAIKAVVDLNGRYFGGRIVKAGFYNLDKF 399
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Sea Lamprey
Match: RBM39 (RNA binding motif protein 39 [Source:HGNC Symbol;Acc:HGNC:15923]) HSP 1 Score: 53.9138 bits (128), Expect = 5.631e-8 Identity = 44/174 (25.29%), Postives = 83/174 (47.70%), Query Frame = 1
Query: 355 VREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALA---QNTVNLYPXXXXXXXXXXXXKVGRPTNMPH--SGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRV 861
+R + + + + G V+ V L D +++ G A++EF ++ LA+ Q + + I Q ++ N+ SGP+ R+YV ++H N++ L +FE FG I++ L D G+ + FGFI + + A +A+ +N F+L G+ ++V
Sbjct: 185 IRAKDLEEFFSAVGKVRDVRLISDRNSRRSKGIAYIEFVDLDSVPLAIGLTGQRLLGVPIIVQASQAEKNRAAAAAAANLQKLASGPM------------RLYVGSLHFNITEAMLMGIFEPFGKIDNIQLMMD-PETGRSKGFGFITFSSADCAKKALEQLNGFELAGRPMKV 345
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Yeast
Match: PAB1 (Poly(A) binding protein; part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G; stimulates, but is not required for the deadenylation activity of the Pan2p-Pan3p poly(A)-ribonuclease complex [Source:SGD;Acc:S000000967]) HSP 1 Score: 64.3142 bits (155), Expect = 2.516e-11 Identity = 50/184 (27.17%), Postives = 87/184 (47.28%), Query Frame = 1
Query: 328 IYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLAL-AQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANK--YNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKA 870
I++ +++ D+ + + + FG + S ++ D K G+ F+ FE AA+ A+ A N + L ++ PH D E K Y +YV N++ + E + +F FGPI S L+ D GK + FGF+ Y+ + A++A+ A+N +L G+ L V +A
Sbjct: 128 IFIKNLHPDIDNKALYDTFSVFGDILSSKIATDE-NGKSKGFGFVHFEEEGAAKEAIDALNGMLLNGQEIYVA--------------PHLSRKERDSQLEETKAHYTNLYVKNINSETTDEQFQELFAKFGPIVSASLEKDAD--GKLKGFGFVNYEKHEDAVKAVEALNDSELNGEKLYVGRA 294
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Yeast
Match: HRP1 (Subunit of cleavage factor I; cleavage factor I is a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences; required for genome stability [Source:SGD;Acc:S000005483]) HSP 1 Score: 60.4622 bits (145), Expect = 3.076e-10 Identity = 39/187 (20.86%), Postives = 81/187 (43.32%), Query Frame = 1
Query: 325 RIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAA-QLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFA----EANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKA 870
++++G +N+D E+ +R+ + +G V + + DP T + G+ FL FE P + ++ Q+ ++ G ++D A E +K +I+V + P++ ++ F +G I L D G+ R FGF+ Y + A++ + D + + + +A
Sbjct: 160 KMFIGGLNWDTTEDNLREYFGKYGTVTDLKIMKDPATGRSRGFGFLSFEKPSSVDEVVKTQHILD--------------------------GKVIDPKRAIPRDEQDKTGKIFVGGIGPDVRPKEFEEFFSQWGTIIDAQLMLDKDT-GQSRGFGFVTYDSAD-AVDRVCQNKFIDFKDRKIEIKRA 318
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Yeast
Match: PUB1 (Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; implicated in regulation of translation; carries Q/N-rich domain at C- terminus, identified as candidate prion; human homolog Tia1 is critical for normal synaptic plasticity; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000004961]) HSP 1 Score: 55.4546 bits (132), Expect = 9.817e-9 Identity = 47/181 (25.97%), Postives = 86/181 (47.51%), Query Frame = 1
Query: 328 IYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKA 870
+YVG+++ + E+ ++Q + GP+ ++ + D K++ YAF+E+ A +AL T+N G I K+ S DD F ++V +++ N+ E LR F+ F S ++ D+ + G R +GF+ + + A A+ +M DL G+ LR+ A
Sbjct: 77 LYVGNLDKAITEDILKQYFQVGGPIANIKIMIDK-NNKNVNYAFVEYHQSHDANIAL--QTLN------GKQIENNIVKINWAFQSQQSS--SDDTF-------NLFVGDLNVNVDDETLRNAFKDFPSYLSGHVMWDM-QTGSSRGYGFVSFTSQDDAQNAMDSMQGQDLNGRPLRINWA 238
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Yeast
Match: RNA15 (Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability [Source:SGD;Acc:S000003012]) HSP 1 Score: 47.7506 bits (112), Expect = 1.991e-6 Identity = 23/57 (40.35%), Postives = 34/57 (59.65%), Query Frame = 1
Query: 328 IYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLAL 498
+Y+GSI YD EE I GPV ++ + +DP T + GYAF+EF E++ A+
Sbjct: 20 VYLGSIPYDQTEEQILDLCSNVGPVINLKMMFDPQTGRSKGYAFIEFRDLESSASAV 76
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Yeast
Match: HSH49 (U2-snRNP associated splicing factor; similar to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) [Source:SGD;Acc:S000005846]) HSP 1 Score: 45.8246 bits (107), Expect = 5.067e-6 Identity = 34/132 (25.76%), Postives = 62/132 (46.97%), Query Frame = 1
Query: 328 IYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALA--QNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPI 717
+YVG+I+ + +E + + + PV + D + Q + GYAF+EF AQ A+ NTV LY + + TN+P + + D+ K +++ N+ ++ S+ L +F FG +
Sbjct: 11 VYVGNIDPRITKEQLYELFIQINPVLRIKYPKDKVLQAYQGYAFIEFYNQGDAQYAIKIMNNTVRLYDRLIKVRQVTNSTGT---TNLPSN--ISKDMILPIAK---LFIKNLADSIDSDQLVKIFNKFGKL 134
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Nematostella
Match: EDO46306 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQK4]) HSP 1 Score: 219.935 bits (559), Expect = 3.367e-65 Identity = 119/259 (45.95%), Postives = 167/259 (64.48%), Query Frame = 1
Query: 166 LKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQ---MINIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPP--------NLVLPGSM 906
L L +++ + +AK+YAMEQS++ VL KQ Q ++ +A+ L+ R+YVGSIN+++REE IR A+ FGP+ + LS+DP+ KH G+AF+E++ PEAAQLAL Q N V L GR KVGRP+N+P + PL++ EA KY RIY+A+VHP+L +D++ VFEAFG + C L + GKH+ +GFIEY+ Q+A +AI +MNLFDLGGQ LRV +AITPP N PG++
Sbjct: 9 LPSLTIIQEELLKRAKRYAMEQSVQQVLAKQQALQAQQMNALQTAAQKQRALALMCRVYVGSINFELREEHIRTAFHPFGPINKIDLSWDPLNMKHKGFAFVEYDLPEAAQLALEQMNGVLL---------GGRNIKVGRPSNVPQAAPLIEQFEQEAKKYARIYIASVHPDLLEDDIKSVFEAFGKVVHCSLSKEPMT-GKHKGYGFIEYENQQSANDAIASMNLFDLGGQFLRVGRAITPPTSSAMAAINTTPPGTL 257 HSP 2 Score: 89.3521 bits (220), Expect = 5.974e-19 Identity = 61/146 (41.78%), Postives = 92/146 (63.01%), Query Frame = 1
Query: 1156 AEIEAM---EVEDKSSKTESLEND-NVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
AEI+A E + K ++ S+ ++ N+ I G AR +MQKL R + KVMVLRNM+ D+D+ +E EV +EC+KFG V V I E + + ++A VKIFV F S D + A ++++GR+FGGR V A+ Y+++ F + D S
Sbjct: 359 AEIKAKIDEETQRKMAEGGSISHEENMSISGSNARYMVMQKLSRKSESKVMVLRNMVGVEDLDEDLEHEVTDECSKFGTVSRVVIYKEKQGEEEDAEVIVKIFVEFTSPDETDKATSNLDGRWFGGRAVKAEAYNEDKFLSADLSG 504
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Nematostella
Match: EDO39879 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8S5]) HSP 1 Score: 68.1662 bits (165), Expect = 2.448e-13 Identity = 39/163 (23.93%), Postives = 72/163 (44.17%), Query Frame = 1
Query: 325 RIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEA 813
++++G +++ E +RQ + FG +K + DP+T++ G+ FL F+ P+A + L L K+ +P P +I++ + N S ED++ F FG I L D +HR FGF+ +++ +A +A
Sbjct: 1 KMFIGGLSWQTTAEGLRQHFEKFGELKECVVMRDPVTKRSRGFGFLTFKDPKAVDVVLNSGAQEL-----------DGKKIDPKLAVPKRAPA-----KMVTTTKKIFIGGLSTNTSEEDMKKYFSQFGKITETMLMFDKATQ-RHRGFGFVTFESENSADKA 146
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Nematostella
Match: EDO33206 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSV8]) HSP 1 Score: 70.0922 bits (170), Expect = 8.600e-13 Identity = 47/166 (28.31%), Postives = 82/166 (49.40%), Query Frame = 1
Query: 319 LSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAI 816
++ +YVG + DV E + + + G V S+ + D +T++ +GYA++ F+ P A+ AL +T+N PI GRP + D + I++ N+ ++ ++ L F AFG I SC + D G + +GF+ ++T AA+EAI
Sbjct: 12 IASLYVGDLAPDVTEAMLYEKFSTAGSVLSIRVCRDLVTRRSLGYAYVNFQQPGHAEKAL--DTMNFDPIK------------GRPCRIMWQ---QRDPSLRKSGVGNIFIKNLDKSIDNKSLYDTFSAFGNILSCKIAQDEL--GNPKGYGFVHFETEDAALEAI 158 HSP 2 Score: 60.8474 bits (146), Expect = 8.476e-10 Identity = 42/184 (22.83%), Postives = 88/184 (47.83%), Query Frame = 1
Query: 319 LSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKA 870
+ I++ +++ + +++ + FG + S ++ D + GY F+ FE +AA A+A+ L + ++ VGR + ++ + + K+ +YV N ++ E ++ + G I S + D GK + FGF+ ++TP+ A EA+ +N ++GG+ L +A
Sbjct: 100 VGNIFIKNLDKSIDNKSLYDTFSAFGNILSCKIAQDELGNPK-GYGFVHFETEDAALEAIARVDGMLL--------NDKKVFVGRWMSKKER---IEKMGTQPKKFTNVYVKNFGDDMDDEQMKEICAEAGKIVSLKVMTDPE--GKSKGFGFVSFETPEEAEEAVNVLNGKEIGGRRLWAGRA 269
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Nematostella
Match: EDO42417 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1S3]) HSP 1 Score: 59.3066 bits (142), Expect = 1.422e-9 Identity = 50/173 (28.90%), Postives = 76/173 (43.93%), Query Frame = 1
Query: 322 SRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLAL-AQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTR---PGKHRAFGFIEYKTPQAAIEAIGAMN 828
+ IYVG ++ V E I + + GPV +V + D ITQ H GY F+EF E A A+ N + +Y G+P + + +L AN +++ N+ + + L F AFG I P + R G + F FI + + A+ AI AMN
Sbjct: 13 ATIYVGGLDEKVSEALIWELFLQSGPVVNVHMPKDRITQLHQGYGFVEFLGEEDADYAIKVMNMIKVY---------------GKPIRVNKASAHNKNLDVGAN----LFIGNLDTEVDEKLLYDTFSAFGVILQT---PKIMRDSDTGNSKGFAFINFASFDASDAAIEAMN 163
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Nematostella
Match: EDO33926 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQW0]) HSP 1 Score: 59.6918 bits (143), Expect = 1.704e-9 Identity = 43/185 (23.24%), Postives = 87/185 (47.03%), Query Frame = 1
Query: 328 IYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKY------NRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVA 864
++ + ++R + + + G V V + D +++ G A++EF A LA+ + L +G P + + + L AEA + R+YV ++H N++ ++ VFE FG ++S L D + + + +GF++++ +AA A+ MN F+L G+ L++
Sbjct: 121 VFCMQLARNIRPRDLEEFFSKVGQVSDVRIISDRNSRRSKGIAYIEFTDKSAVPLAIGLSGQKL---------------LGAPIMVMLTQAEKNRLAAEAERLKQPLGPTRLYVGSLHFNITEAMVKAVFEPFGTVDSVQLIYD-SETNRSKGYGFVQFREAEAAKRAMEQMNGFELAGRPLKIG 289 HSP 2 Score: 54.6842 bits (130), Expect = 5.978e-8 Identity = 24/61 (39.34%), Postives = 39/61 (63.93%), Query Frame = 1
Query: 322 SRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ 504
+R+YVGS+++++ E ++ + FG V SV L YD T + GY F++F EAA+ A+ Q
Sbjct: 215 TRLYVGSLHFNITEAMVKAVFEPFGTVDSVQLIYDSETNRSKGYGFVQFREAEAAKRAMEQ 275
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Medaka
Match: PUF60 (poly(U) binding splicing factor 60 [Source:NCBI gene;Acc:101163935]) HSP 1 Score: 271.552 bits (693), Expect = 2.423e-84 Identity = 138/269 (51.30%), Postives = 191/269 (71.00%), Query Frame = 1
Query: 127 EGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK----RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
+G GS L P L +++A+ +AKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E++ PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA YNRIYVA+VHP+LS +D++ VFEAFG I+SC L D T G+HR FGFIEY+ PQ+A++A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 18 QGTGSKLGLPP-----LTPEQQEALQRAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYDVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAYNRIYVASVHPDLSDDDIKSVFEAFGRIKSCMLARDPTS-GRHRGFGFIEYEKPQSAVDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPTTPGGL 271 HSP 2 Score: 98.2117 bits (243), Expect = 2.217e-21 Identity = 60/135 (44.44%), Postives = 88/135 (65.19%), Query Frame = 1
Query: 1177 VEDKSSKTESLENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
++D + + + +++ I G AR+ +MQKLLR + VMVLRNM+ DIDD +E EV EEC KFG V V I E + + ++A+ VKIFV F + +N AI ++N R+FGGR V+A+ YDQ+ F++ D SA
Sbjct: 375 LQDGTGQEMLSDQEHMSISGSSARHMVMQKLLRKSESTVMVLRNMVGPEDIDDDLEGEVTEECGKFGTVNRVIIYQEKQGEEEDADIIVKIFVEFSAASEMNKAIQALNDRWFGGRKVVAEVYDQDRFNSSDLSA 509
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Medaka
Match: PUF60 (poly(U) binding splicing factor 60 [Source:NCBI gene;Acc:101163935]) HSP 1 Score: 265.003 bits (676), Expect = 8.388e-82 Identity = 138/286 (48.25%), Postives = 191/286 (66.78%), Query Frame = 1
Query: 127 EGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK---------------------RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
+G GS L P L +++A+ +AKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E++ PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA YNRIYVA+VHP+LS +D++ VFEAFG I+SC L D T G+HR FGFIEY+ PQ+A++A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 5 QGTGSKLGLPP-----LTPEQQEALQRAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYDVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAYNRIYVASVHPDLSDDDIKSVFEAFGRIKSCMLARDPTS-GRHRGFGFIEYEKPQSAVDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPTTPGGL 275 HSP 2 Score: 98.2117 bits (243), Expect = 2.066e-21 Identity = 60/135 (44.44%), Postives = 88/135 (65.19%), Query Frame = 1
Query: 1177 VEDKSSKTESLENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
++D + + + +++ I G AR+ +MQKLLR + VMVLRNM+ DIDD +E EV EEC KFG V V I E + + ++A+ VKIFV F + +N AI ++N R+FGGR V+A+ YDQ+ F++ D SA
Sbjct: 379 LQDGTGQEMLSDQEHMSISGSSARHMVMQKLLRKSESTVMVLRNMVGPEDIDDDLEGEVTEECGKFGTVNRVIIYQEKQGEEEDADIIVKIFVEFSAASEMNKAIQALNDRWFGGRKVVAEVYDQDRFNSSDLSA 513
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Medaka
Match: PUF60 (poly(U) binding splicing factor 60 [Source:NCBI gene;Acc:101163935]) HSP 1 Score: 265.388 bits (677), Expect = 9.296e-82 Identity = 138/286 (48.25%), Postives = 191/286 (66.78%), Query Frame = 1
Query: 127 EGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIK---------------------RTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL----PGSM 906
+G GS L P L +++A+ +AKKYAMEQSI++VL+KQT H+ QQ+ N++ R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E++ PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA YNRIYVA+VHP+LS +D++ VFEAFG I+SC L D T G+HR FGFIEY+ PQ+A++A+ +MNLFDLGGQ LRV KA+TPP +L PG +
Sbjct: 18 QGTGSKLGLPP-----LTPEQQEALQRAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYDVPEAAQLALEQMNSVML---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAYNRIYVASVHPDLSDDDIKSVFEAFGRIKSCMLARDPTS-GRHRGFGFIEYEKPQSAVDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPTTPGGL 288 HSP 2 Score: 98.5969 bits (244), Expect = 1.988e-21 Identity = 60/135 (44.44%), Postives = 88/135 (65.19%), Query Frame = 1
Query: 1177 VEDKSSKTESLENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
++D + + + +++ I G AR+ +MQKLLR + VMVLRNM+ DIDD +E EV EEC KFG V V I E + + ++A+ VKIFV F + +N AI ++N R+FGGR V+A+ YDQ+ F++ D SA
Sbjct: 392 LQDGTGQEMLSDQEHMSISGSSARHMVMQKLLRKSESTVMVLRNMVGPEDIDDDLEGEVTEECGKFGTVNRVIIYQEKQGEEEDADIIVKIFVEFSAASEMNKAIQALNDRWFGGRKVVAEVYDQDRFNSSDLSA 526
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Medaka
Match: ENSORLT00000038253.1 (poly(U)-binding-splicing factor PUF60-like [Source:NCBI gene;Acc:101171279]) HSP 1 Score: 255.373 bits (651), Expect = 1.700e-78 Identity = 126/249 (50.60%), Postives = 179/249 (71.89%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQT---KQHEVQQMINIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL 894
L L ++++A+ +AKKYAMEQSI++VL+KQT +Q + +R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA +NRIYVA+VHP+LS ED++ VFEAFG I+SC L + T G+H+ +GFIEY PQ++++A+ +MNLFDLGGQ LRV KA+TPP +L
Sbjct: 14 LGLPPLTPTQQEALQRAKKYAMEQSIKSVLMKQTLVQQQQFTSLQVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEMPEAAQLALEQMNSVVL---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHPDLSDEDIKSVFEAFGRIKSCMLAREPTT-GRHKGYGFIEYDKPQSSLDAVASMNLFDLGGQYLRVGKAVTPPMPLL 252 HSP 2 Score: 80.1073 bits (196), Expect = 1.207e-15 Identity = 55/123 (44.72%), Postives = 75/123 (60.98%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYS 1575
E ++V I G AR+ + VMVLRNM+ DIDD +E EV EEC K+G+V+ V I E + + D+A+ VKIFV F +N AI ++N R+FGGR V+A+ YDQE F+ D S
Sbjct: 367 EQEHVSISGSSARH----------MSTVMVLRNMVGPEDIDDDLEGEVTEECGKYGQVKRVIIYQERQGEEDDADIIVKIFVEFCEAMEMNRAIQALNNRWFGGRKVVAEVYDQERFENSDLS 479
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Medaka
Match: ENSORLT00000038235.1 (poly(U)-binding-splicing factor PUF60-like [Source:NCBI gene;Acc:101171279]) HSP 1 Score: 248.825 bits (634), Expect = 1.301e-75 Identity = 127/270 (47.04%), Postives = 180/270 (66.67%), Query Frame = 1
Query: 160 FNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQ------------------------QMINIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQ-NTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVL 894
L L ++++A+ +AKKYAMEQSI++VL+KQT + Q Q + +R +A+ ++ R+YVGSI Y++ E+TIRQA+ FGP+KS+ +S+D +T KH G+AF+E+E PEAAQLAL Q N+V L GR KVGRP+N+ + P++D L EA +NRIYVA+VHP+LS ED++ VFEAFG I+SC L + T G+H+ +GFIEY PQ++++A+ +MNLFDLGGQ LRV KA+TPP +L
Sbjct: 14 LGLPPLTPTQQEALQRAKKYAMEQSIKSVLMKQTLVQQQQFTSLQVRALMASLTMGIGDALSPLQSVAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEMPEAAQLALEQMNSVVL---------GGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHPDLSDEDIKSVFEAFGRIKSCMLAREPTT-GRHKGYGFIEYDKPQSSLDAVASMNLFDLGGQYLRVGKAVTPPMPLL 273 HSP 2 Score: 95.1301 bits (235), Expect = 2.018e-20 Identity = 60/123 (48.78%), Postives = 81/123 (65.85%), Query Frame = 1
Query: 1210 ENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESR-DNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYS 1575
E ++V I G AR+ +M+KLLR VMVLRNM+ DIDD +E EV EEC K+G+V+ V I E + + D+A+ VKIFV F +N AI ++N R+FGGR V+A+ YDQE F+ D S
Sbjct: 392 EQEHVSISGSSARHMVMRKLLRKRESTVMVLRNMVGPEDIDDDLEGEVTEECGKYGQVKRVIIYQERQGEEDDADIIVKIFVEFCEAMEMNRAIQALNNRWFGGRKVVAEVYDQERFENSDLS 514
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Planmine SMEST
Match: SMESG000046155.1 (SMESG000046155.1) HSP 1 Score: 853.588 bits (2204), Expect = 0.000e+0 Identity = 497/498 (99.80%), Postives = 498/498 (100.00%), Query Frame = 1
Query: 85 MHFLPVLEQYGSILEGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVLPGSMXXXXXXXXXXXXXXXXXXXXHTLEVEQVVKVEEAGTXXXXXXXXXXXXXXXXXXXXVLPPTMIIPDCSSMSSQINEKVAMRSAAEIEAMEVEDKSSKTESLENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESRDNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
MHFLPVLEQYGSILEGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPISQGISISGRQSKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVLPGSMVTSSMPAATAVAAASITAQVHTLEVEQVVKVEEAGTKANVSKEKKKKFSDSKVADSVLPPTMIIPDCSSMSSQINEKVAMRSAAEIEAMEVEDKSSKTESLENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMIDQNDIDDQIEEEVYEECAKFGEVQEVKIV+ESRDNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA
Sbjct: 1 MHFLPVLEQYGSILEGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPISQGISISGRQSKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVLPGSMVTSSMPAATAVAAASITAQVHTLEVEQVVKVEEAGTKANVSKEKKKKFSDSKVADSVLPPTMIIPDCSSMSSQINEKVAMRSAAEIEAMEVEDKSSKTESLENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMIDQNDIDDQIEEEVYEECAKFGEVQEVKIVVESRDNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 498
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Planmine SMEST
Match: SMESG000046155.1 (SMESG000046155.1) HSP 1 Score: 837.41 bits (2162), Expect = 0.000e+0 Identity = 489/498 (98.19%), Postives = 490/498 (98.39%), Query Frame = 1
Query: 85 MHFLPVLEQYGSILEGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVLPGSMXXXXXXXXXXXXXXXXXXXXHTLEVEQVVKVEEAGTXXXXXXXXXXXXXXXXXXXXVLPPTMIIPDCSSMSSQINEKVAMRSAAEIEAMEVEDKSSKTESLENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIVLESRDNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 1578
MHFLPVLEQYGSILEGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNT GISISGRQSKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVLPGSMVTSSMPAATAVAAASITAQVHTLEVEQVVKVEEAGTKANVSKEKKKKFSDSKVADSVLPPTMIIPDCSSMSSQINEKVAMRSAAEIEAMEVEDKSSKTESLENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMIDQNDIDDQIEEEVYEECAKFGEVQEVKIV+ESRDNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA
Sbjct: 1 MHFLPVLEQYGSILEGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSIRTVLLKQTKQHEVQQMINIKRTQAMVLLSRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNT--------GISISGRQSKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAITPPNLVLPGSMVTSSMPAATAVAAASITAQVHTLEVEQVVKVEEAGTKANVSKEKKKKFSDSKVADSVLPPTMIIPDCSSMSSQINEKVAMRSAAEIEAMEVEDKSSKTESLENDNVKIRGREARNQMMQKLLRPTVHKVMVLRNMIDQNDIDDQIEEEVYEECAKFGEVQEVKIVVESRDNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELFDAGDYSA 490
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Planmine SMEST
Match: SMESG000058745.1 (SMESG000058745.1) HSP 1 Score: 63.1586 bits (152), Expect = 2.697e-10 Identity = 48/182 (26.37%), Postives = 88/182 (48.35%), Query Frame = 1
Query: 328 IYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALAQNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAI 873
+YVG ++ DV E + + + G V S+ + D +T+K +GYA++ ++ A+ A+ + +N I GRP + S D A + I++ N+ P+++ +++ F FG I SC +Q D G + F F+ + A +AI A+N + +++ VAK I
Sbjct: 11 LYVGDLHPDVTEAILYEKFQTHGNVLSIRVCRDLVTRKSLGYAYVNYQVSTEAKSAM--DNLNFEDIH------------GRPCRIMWS---QRDPAARRSGVGNIFIKNLDPSITVKEINDTFANFGEISSCKVQTDEN--GVSKGFAFVNFNHEGDAEKAIQAINGKMIREKIVYVAKFI 173
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Planmine SMEST
Match: SMESG000005564.1 (SMESG000005564.1) HSP 1 Score: 61.2326 bits (147), Expect = 4.933e-10 Identity = 55/186 (29.57%), Postives = 82/186 (44.09%), Query Frame = 1
Query: 322 SRIYVGSINYDVREETIRQAYCCFGPVKSVSLSYDPITQKHMGYAFLEFEYPEAAQLALA-QNTVNLYPXXXXXXXXXXXXKVGRPTNMPHSGPLVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMNLFDLGGQLLRVAKAIT 876
+ IYVG ++ V E + + + GPV +V + D +T H GY F+EF E A A+ N + LY G+P + + +L AN I++ N+ P + + L F AFG I T G + + FI Y + +A+ AI AMN GQ L +AIT
Sbjct: 13 ASIYVGGLDGKVTEAILWELFLQAGPVVNVHMPKDRVTGLHQGYGFVEFLGEEDADYAMKIMNMIKLY---------------GKPIRVNKASSHRKNLDIGAN----IFIGNLDPEVDEKLLYDTFSAFGVILQTPKIMRETESGNSKGYAFINYASFEASDAAIEAMN-----GQYL-CNRAIT 173
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Planmine SMEST
Match: SMESG000038359.1 (SMESG000038359.1) HSP 1 Score: 61.2326 bits (147), Expect = 8.240e-10 Identity = 42/101 (41.58%), Postives = 60/101 (59.41%), Query Frame = 1
Query: 1258 MQKLLRPTVHKVMVLRNMXXXXXXXXXXXXXVYEECAKFGEVQEVKIV-LESRDNDNANGEVKIFVVFKSTDSINIAINSMNGRFFGGRIVIADKYDQELF 1557
+Q L+ V V++LRNM+ ++DD ++ E EEC+K+G+V I L+ +D A V+IFV F +S A+ NGR+FGGRIV A YD E F
Sbjct: 285 IQSLISDRVSNVILLRNMVGPGEVDDDLQSETAEECSKYGKVVTCMIFELQDVPDDQA---VRIFVEFVDVESAYRAVVDTNGRYFGGRIVSAGYYDSEKF 382 The following BLAST results are available for this feature:
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Poly(U)-binding-splicing factor PUF60 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Poly(U)-binding-splicing factor PUF60 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Poly(U)-binding-splicing factor PUF60 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30001529 ID=SMED30001529|Name=Poly(U)-binding-splicing factor PUF60|organism=Schmidtea mediterranea sexual|type=transcript|length=1624bpback to top protein sequence of SMED30001529-orf-1 >SMED30001529-orf-1 ID=SMED30001529-orf-1|Name=SMED30001529-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=499bp MHFLPVLEQYGSILEGVGSNKLLEPFNLKRLNSSEKDAINKAKKYAMEQSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
