Vacuolar protein sorting-associated protein 13A/C

Overview
NameVacuolar protein sorting-associated protein 13A/C
Smed IDSMED30001501
Length (bp)11021
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Vacuolar protein sorting-associated protein 13A/C (SMED30001501) t-SNE clustered cells

Violin plots show distribution of expression levels for Vacuolar protein sorting-associated protein 13A/C (SMED30001501) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Vacuolar protein sorting-associated protein 13A/C (SMED30001501) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Vacuolar protein sorting-associated protein 13A/C (SMED30001501) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30001501

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30001501SMESG000037244.1 dd_Smed_v4_1648_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neuronSMED30001501SMESG000037244.1 dd_Smed_v4_1648_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30001501SMESG000037244.1 dd_Smed_v4_1648_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
pigment cellSMED30001501SMESG000037244.1 dd_Smed_v6_1648_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30001501SMESG000037244.1 dd_Smed_v6_1648_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
secretory systemSMED30001501SMESG000037244.1 dd_Smed_v6_1648_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Human
Match: VPS13A (vacuolar protein sorting 13 homolog A [Source:HGNC Symbol;Acc:HGNC:1908])

HSP 1 Score: 1227.23 bits (3174), Expect = 0.000e+0
Identity = 1026/3439 (29.83%), Postives = 1665/3439 (48.42%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKE-TETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFF-KSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSS-------SLASDGSDELQKKVRSE-----FTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGS-VPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSE-RTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQ-----TAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSN--FNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDE-----------SITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELI--RNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLE---VYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFS--PKDNEGPSRGNVSEREIN---TLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPT-----LLTSNGKIYISVKQLNTE--ISNSGALWNIG-KNAQPSDTQILLC----KSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISY-----QSQIFSHDIKPGCLELVRTACL-KVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQSLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFY---ESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQ-ELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSN-NLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKW-NQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVE-GLEVDLSKK----LMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEA-MFVKDCQMIR-----ASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA--------LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLF 10067
            MVFES+VV+++N++LGDYVV LD SQL LG+  G   L+NL IKENA    D+P KV+ GHI  L L IPWK+LY++PV A L+ +Y+LI+P+S +KYDP KE   L E KQ+EL RIEE K K  D ++   E +D+F EKL  QI+KNLQ+ IS+IHIRYED  T  ++  + GI+L NLS QT D      +   D T +   K +++D+L  YWN   + F   +       +KN I +         F+ +PIS +A + ++ + +  +F+ P+++L+++L  I   FN+ QY  I   L+S+D       +RK++P+VP+  +   WW +  + +LE  V  R  MWSWK+I+ HR + + Y +++++KL  KK       +++  E+ LD+ NIT+ RQ AE++ KK   K+ ++              D E +        S +   ++E Q  V+ E      T EEKA LY+AIGYSE   DP +   + +LK  + L+ +  ++ +N  K  +L+ +VI   S  + QR  A AI     +++  +TG   PD S  P L+S+   +  M+L  + FE NPLD++ +Q+  I AEPLEIIYD+ TVN IV+F  PP+E+ L +++   L  +EE +  TATGL ++ + +   D+K+++K SY I+P+ G ++    +LL+DLG + + S+ R+ +P          K G+ N   + + AYD F I L+++Q L    GD+WR+ R    S  HIL P  F   L K ++  D  +PK  + G LP + + ISD+ LQ ++ELI  IP P+     +AP K      S     S     I P L L S     +     D+  + L   +             L+  D +V              NM      F +    ++    + +  +       +V+++V  +G  + ++ +D+   A++    L  P+++D   K PV L  T  +T  ++LL +  +  EK  PD  + + N    ++  F S+++ LH EALL  +N++++++P      EEK+   S+         T  E + + I+ L                + L+T +  + L+                              I A L  L++ + ++K  NI +I I G+ +++      TE++  + ++ + D    A+YKK ++I   E +FS ++  +   T+   Y  D++ VD  VN+ VG I+++F+  F+  I  F+++FQ + Q +A    + +  AA  +  + +   SSR  LDIN+KAPV+ IP++  S N  + D G +T  N F +      S   V++   I LS +R+ RS  + +  +    +L P+N+ + + RNL W     +P  ++ A++  +   +SQ D       L  N  + +  +  P+V + +    S         SG A   +               T+   FK + L + L+        +     P   L + K EN+     M+ + +   S  L N  +LD +    KK    +    +  D  D + IK+ + ++      V    +Y+C  V++L  + + F++A                  E + + +  K      E     +EI+V +  PEI+ V DM   D+ A+++ T   + Y    E+  +   I D Q   CPF    RK     ++ P  +     +   +     ++++ L + ++P +I ++  I + L   +T ET       I  +      + WEK++    +  F+ +   E EKI    + V  G                                I + +  I ++LE+ +  + VPML+ +    G+ KN  + I L   + + V Y+N+   VWEPLLE   +   E  R ++  I+        + + +  E+   +      I   S+D L +T+SK  L +L+NL KAF +A +           AP+++ N+ G  +    +  F +         K Y+  +GE    D +  K +            F    S+S +   I  T V        P    G     V  RE     +++       G K++ +RS +Q+ N+    +++Y            +   LL        +N+P+ +      L    + Y   + ++ E  I N GAL     ++  PS    L+     K N   +  YS                   Y + +  P+ L N LP KI+Y     ++ +F+  +  G    + TA L K + ++K+   Y   +W     + P  +++S ++F       ++ +D+ VH+    GQ  V  +SPYWM+N        K G   Q + D                           I +K    Y    L + +                  N+  N N       K  L + DS  S++FS+DTVGS G V CK   + Y+VGV I+LSS  +T+IVTF P+YMI N+S   +   E   N  W+ +   + +PFWP     K ++   + ED       P   Y   + + IL++L+ +  GI  EV + E + +I    Y +G A   LINH  +++ + + Q +  + E  LP  + V Y W D    R   WR    +K H       D +    + E   Y VSF     RI+LFT D  V K    S   E  +QEI ++L  +G+SLV+N  +QE+AYI ITSS V W  + ++K R KP+ +  +E LE  F E+     T  +  E+ +++++  + V L+       +++P    L R + P + ++Y TS HQ    ++IY+IQI NQ+    FP VF P  PP+SV +D+  KP  ++S V+  +G +   R KYF+VLIQEM L LD GF+ ++ D ++ +  T + E  +F KD +  +     ASL++ S +          +++ FHISPIK+HLS SLS      K +        +HS  LNL L+S+G  +TD+QDV+FKL +FE        + + S +  HY  QA++QMYVL+LGLDVLGNPFG+IR  ++GVE  FYEP +GA+ GPEEFVEG+A G+K+L G AVGG AGA S+I G M KG+AA+T+D DYQ+KRRE + K+P     G+ +GGKGL+ GFV G+TG+VTKPI GA+K G  GFFKGVGKGLVG V RP  G++D ASS+F+GI+R  +T+ EV  +RPPRF  +DG+IR Y LR+  GN + +++ E         Y  HV   +K +++ +T + +  + +    G  + +WQ ++++    P I   +  +    KE+ + +F
Sbjct:    1 MVFESVVVDVLNRFLGDYVVDLDTSQLSLGIWKGAVALKNLQIKENALSQLDVPFKVKVGHIGNLKLIIPWKNLYTQPVEAVLEEIYLLIVPSSRIKYDPLKEEKQLMEAKQQELKRIEEAKQKVVDQEQHLPEKQDTFAEKLVTQIIKNLQVKISSIHIRYEDDITNRDKPLSFGISLQNLSMQTTDQYWVPCL--HDETEKLVRKLIRLDNLFAYWNVKSQMFYLSDYDNSLDDLKNGIVNENIVPEGYDFVFRPISANAKLVMNRRSDF-DFSAPKINLEIELHNIAIEFNKPQYFSIMELLESVDMMAQNLPYRKFKPDVPLHHHAREWWAYAIHGVLEVNVCPRLWMWSWKHIRKHRQKVKQYKELYKKKLTSKKPPGELLVSLEELEKTLDVFNITIARQTAEVEVKKAGYKIYKEGVK-----------DPEDNKGWFSWLWSWSEQNTNEQQPDVQPETLEEMLTPEEKALLYEAIGYSETAVDPTLLKTFEALKFFVHLKSMSIVLRENHQKP-ELVDIVIEEFSTLIVQRPGAQAIKFETKIDSFHITGL--PDNSEKPRLLSSLDDA--MSLFQITFEINPLDETVSQRCIIEAEPLEIIYDARTVNSIVEFFRPPKEVHLAQLTAATLTKLEEFRSKTATGLLYIIETQKVLDLKINLKASYIIVPQDGIFSPTSNLLLLDLGHLKVTSKSRSELPDV--------KQGEANLKEIMDRAYDSFDIQLTSVQLLYSRVGDNWREARKLSVSTQHILVPMHFNLELSKAMVFMDVRMPKFKIYGKLPLISLRISDKKLQGIMELIESIPKPEPVTEVSAPVKSFQIQTSTSLGTSQISQKIIPLLELPSVSEDDSEEEFFDAPCSPLEEPLQFPTGVKSIRTRKLQKQDCSV--------------NMTTFKIRFEVPKVLIEFYHLVGDCELS------VVEILVLGLGAEIEIRTYDLKANAFLKEFCLKCPEYLD-ENKKPVYLVTTLDNTM-EDLLTLEYVKAEKNVPDLKSTYNNVLQLIKVNFSSLDIHLHTEALLNTINYLHNILPQ----SEEKSAPVST---------TETEDKGDVIKKLA---------------LKLSTNEDIITLQ------------------------------ILAELSCLQIFIQDQK-CNISEIKIEGLDSEMIMRPSETEINAKLRNIIVLDSDITAIYKKAVYITGKE-VFSFKMVSYMDATAGSAYT-DMNVVDIQVNLIVGCIEVVFVTKFLYSILAFIDNFQAAKQALAEATVQAAGMAATGVKELAQR--SSRMALDINIKAPVVVIPQSPVSENVFVADFGLITMTNTFHMITESQSSPPPVIDLITIKLSEMRLYRSRFINDAYQEVLDLLLPLNLEVVVERNLCWEWYQEVPCFNVNAQLKPMEFILSQEDITTIFKTLHGNIWYEKDGSASPAVTKDQYSATSGVTTNASHHSGGATVVTAAVVEVHSRALLVKTTLNISFKTDDLTMVLYSPGPKQASFTDVRDPSLKLAEFKLENIISTLKMYTDGSTFSSFSLKNC-ILDDKRPHVKKATPRMIGLTVGFDKKDMMDIKYRKVRDGCVTDAV-FQEMYICASVEFLQTVANVFLEAYTTGT-----------AVETSVQTWTAKEEVPTQES--VKWEINVIIKNPEIVFVADMTKNDAPALVITTQCEICYKGNLENSTMTAAIKDLQVRACPFLPVKRKGKITTVLQPCDLFYQTTQKGTDPQVIDMSVKSLTLKVSPVIINTMITITSAL--YTTKET-------IPEETASSTAHLWEKKDTKTLKMWFLEES-NETEKIAPTTELVPKG------------------------------EMIKMNIDSIFIVLEAGIGHRTVPMLLAKSRFSGEGKNWSSLINLHCQLELEVHYYNEMFGVWEPLLEPLEIDQTEDFRPWNLGIKMKKKAKMAIVESDPEEENYKVPEYKTVISFHSKDQLNITLSKCGLVMLNNLVKAFTEAATGSSADFVKDL-APFMILNSLGLTISVSPSDSFSVLNIPMA---KSYVLKNGESLSMDYIRTKDN----------DHFNAMTSLSSKLFFILLTPVNHSTADKIPLTKVGRRLYTVRHRESGVERSIVCQIDTVEGSKKVTIRSPVQIRNHFSVPLSVY------------EGDTLLGTASPENEFNIPLGSYRSFIFLKPEDENYQMCEGIDFEEIIKNDGALLKKKCRSKNPSKESFLINIVPEKDNLTSLSVYS------------EDGWDLPYIMHLWPPILLRNLLPYKIAYYIEGIENSVFT--LSEGHSAQICTAQLGKARLHLKL-LDYLNHDWKSEYHIKPNQQDISFVSFTCVTEMEKTDLDIAVHMTYNTGQTVVAFHSPYWMVN--------KTGRMLQYKADG--------------------------IHRKHPPNYKKPVLFSFQP-----------------NHFFNNN-------KVQLMVTDSELSNQFSIDTVGSHGAVKCKGLKMDYQVGVTIDLSSFNITRIVTFTPFYMIKNKSKYHISVAE-EGNDKWLSLDLEQCIPFWPEYASSKLLIQVERSED------PPKRIYFNKQENCILLRLDNELGGIIAEVNLAEHSTVITFLDYHDGAATFLLINHTKNEL-VQYNQSSLSEIEDSLPPGKAVFYTWADPVGSRRLKWRC---RKSHGEVTQKDDMMMPIDLGEKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAEQEIAVALQDVGISLVNNYTKQEVAYIGITSSDVVWETKPKKKARWKPMSVKHTEKLEREFKEY-----TESSPSEDKVIQLDTNVPVRLTPTGHNMKILQPHVIALRRNYLPALKVEYNTSAHQSSFRIQIYRIQIQNQIHGAVFPFVFYPVKPPKSVTMDSAPKPFTDVSIVMRSAGHSQISRIKYFKVLIQEMDLRLDLGFIYALTDLMTEAEVTENTEVELFHKDIEAFKEEYKTASLVDQSQV---------SLYEYFHISPIKLHLSVSLSSGREEAKDSKQNGGLIPVHS--LNLLLKSIGATLTDVQDVVFKLAFFELNYQFHTTSDLQSEVIRHYSKQAIKQMYVLILGLDVLGNPFGLIREFSEGVEAFFYEPYQGAIQGPEEFVEGMALGLKALVGGAVGGLAGAASKITGAMAKGVAAMTMDEDYQQKRREAMNKQPAGFREGITRGGKGLVSGFVSGITGIVTKPIKGAQKGGAAGFFKGVGKGLVGAVARPTGGIIDMASSTFQGIKRATETS-EVESLRPPRFFNEDGVIRPYRLRDGTGNQM-LQVMENGR-FAKYKYFTHVMI-NKTDMLMITRRGVLFVTKG-TFGQLTCEWQYSFDEFTKEPFIVHGRR-LRIEAKERVKSVF 3140          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Human
Match: VPS13A (vacuolar protein sorting 13 homolog A [Source:HGNC Symbol;Acc:HGNC:1908])

HSP 1 Score: 1222.22 bits (3161), Expect = 0.000e+0
Identity = 1012/3358 (30.14%), Postives = 1631/3358 (48.57%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKE-TETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSS-------SLASDGSDELQKKVRSE-----FTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGS-VPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSE-RTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQ-----TAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSN--FNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDE-----------SITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELI--RNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLE---VYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFS--PKDNEGPSRGNVSEREIN---TLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPT-----LLTSNGKIYISVKQLNTE--ISNSGALWNIG-KNAQPSDTQILLC----KSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISY-----QSQIFSHDIKPGCLELVRTACL-KVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQSLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFY---ESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQ-ELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSN-NLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKW-NQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVE-GLEVDLSKK----LMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEA-MFVKDCQMIR-----ASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA--------LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKI 9827
            MVFES+VV+++N++LGDYVV LD SQL LG+  G   L+NL IKENA    D+P KV+ GHI  L L IPWK+LY++PV A L+ +Y+LI+P+S +KYDP KE   L E KQ+EL RIEE K K  D ++   E +D+F EKL  QI+KNLQ+ IS+IHIRYED  T  ++  + GI+L NLS QT D      +  +++ +   K +++D+L  YWN   + F   +       +KN I +         F+ +PIS +A + ++ + +  +F+ P+++L+++L  I   FN+ QY  I   L+S+D       +RK++P+VP+  +   WW +  + +LE  V  R  MWSWK+I+ HR + + Y +++++KL  KK       +++  E+ LD+ NIT+ RQ AE++ KK   K+ ++              D E +        S +   ++E Q  V+ E      T EEKA LY+AIGYSE   DP +   + +LK  + L+ +  ++ +N  K  +L+ +VI   S  + QR  A AI     +++  +TG   PD S  P L+S+   +  M+L  + FE NPLD++ +Q+  I AEPLEIIYD+ TVN IV+F  PP+E+ L +++   L  +EE +  TATGL ++ + +   D+K+++K SY I+P+ G ++    +LL+DLG + + S+ R+ +P          K G+ N   + + AYD F I L+++Q L    GD+WR+ R    S  HIL P  F   L K ++  D  +PK  + G LP + + ISD+ LQ ++ELI  IP P+     +AP K      S     S     I P L L S     +     D+  + L   +             L+  D +V              NM      F +    ++    + +  +       +V+++V  +G  + ++ +D+   A++    L  P+++D   K PV L  T  +T  ++LL +  +  EK  PD  + + N    ++  F S+++ LH EALL  +N++++++P      EEK+   S+         T  E + + I+ L                + L+T +  + L+                              I A L  L++ + ++K  NI +I I G+ +++      TE++  + ++ + D    A+YKK ++I   E +FS ++  +   T+   Y  D++ VD  VN+ VG I+++F+  F+  I  F+++FQ + Q +A    + +  AA  +  + +   SSR  LDIN+KAPV+ IP++  S N  + D G +T  N F +      S   V++   I LS +R+ RS  + +  +    +L P+N+ + + RNL W     +P  ++ A++  +   +SQ D       L  N  + +  +  P+V + +    S         SG A   +               T+   FK + L + L+        +     P   L + K EN+     M+ + +   S  L N  +LD +    KK    +    +  D  D + IK+ + ++      V    +Y+C  V++L  + + F++A                  E + + +  K      E     +EI+V +  PEI+ V DM   D+ A+++ T   + Y    E+  +   I D Q   CPF    RK     ++ P  +     +   +     ++++ L + ++P +I ++  I + L   +T ET       I  +      + WEK++    +  F+ +   E EKI    + V  G                                I + +  I ++LE+ +  + VPML+ +    G+ KN  + I L   + + V Y+N+   VWEPLLE   +   E  R ++  I+        + + +  E+   +      I   S+D L +T+SK  L +L+NL KAF +A +           AP+++ N+ G  +    +  F +         K Y+  +GE    D +  K +            F    S+S +   I  T V        P    G     V  RE     +++       G K++ +RS +Q+ N+    +++Y            +   LL        +N+P+ +      L    + Y   + ++ E  I N GAL     ++  PS    L+     K N   +  YS                   Y + +  P+ L N LP KI+Y     ++ +F+  +  G    + TA L K + ++K+   Y   +W     + P  +++S ++F       ++ +D+ VH+    GQ  V  +SPYWM+N        K G   Q + D                           I +K    Y    L + +                  N+  N N       K  L + DS  S++FS+DTVGS G V CK   + Y+VGV I+LSS  +T+IVTF P+YMI N+S   +   E   N  W+ +   + +PFWP     K ++   + ED       P   Y   + + IL++L+ +  GI  EV + E + +I    Y +G A   LINH  +++ + + Q +  + E  LP  + V Y W D    R   WR    +K H       D +    + E   Y VSF     RI+LFT D  V K    S   E  +QEI ++L  +G+SLV+N  +QE+AYI ITSS V W  + ++K R KP+ +  +E LE  F E+     T  +  E+ +++++  + V L+       +++P    L R + P + ++Y TS HQ    ++IY+IQI NQ+    FP VF P  PP+SV +D+  KP  ++S V+  +G +   R KYF+VLIQEM L LD GF+ ++ D ++ +  T + E  +F KD +  +     ASL++ S +          +++ FHISPIK+HLS SLS      K +        +HS  LNL L+S+G  +TD+QDV+FKL +FE        + + S +  HY  QA++QMYVL+LGLDVLGNPFG+IR  ++GVE  FYEP +GA+ GPEEFVEG+A G+K+L G AVGG AGA S+I G M KG+AA+T+D DYQ+KRRE + K+P     G+ +GGKGL+ GFV G+TG+VTKPI GA+K G  GFFKGVGKGLVG V RP  G++D ASS+F+GI+R  +T+ EV  +RPPRF  +DG+IR Y LR+  GN +  KI
Sbjct:    1 MVFESVVVDVLNRFLGDYVVDLDTSQLSLGIWKGAVALKNLQIKENALSQLDVPFKVKVGHIGNLKLIIPWKNLYTQPVEAVLEEIYLLIVPSSRIKYDPLKEEKQLMEAKQQELKRIEEAKQKVVDQEQHLPEKQDTFAEKLVTQIIKNLQVKISSIHIRYEDDITNRDKPLSFGISLQNLSMQTTDQYWVPCL-HDETEKLVRKLIRLDNLFAYWNVKSQMFYLSDYDNSLDDLKNGIVNENIVPEGYDFVFRPISANAKLVMNRRSDF-DFSAPKINLEIELHNIAIEFNKPQYFSIMELLESVDMMAQNLPYRKFKPDVPLHHHAREWWAYAIHGVLEVNVCPRLWMWSWKHIRKHRQKVKQYKELYKKKLTSKKPPGELLVSLEELEKTLDVFNITIARQTAEVEVKKAGYKIYKEGVK-----------DPEDNKGWFSWLWSWSEQNTNEQQPDVQPETLEEMLTPEEKALLYEAIGYSETAVDPTLLKTFEALKFFVHLKSMSIVLRENHQKP-ELVDIVIEEFSTLIVQRPGAQAIKFETKIDSFHITGL--PDNSEKPRLLSSLDDA--MSLFQITFEINPLDETVSQRCIIEAEPLEIIYDARTVNSIVEFFRPPKEVHLAQLTAATLTKLEEFRSKTATGLLYIIETQKVLDLKINLKASYIIVPQDGIFSPTSNLLLLDLGHLKVTSKSRSELPDV--------KQGEANLKEIMDRAYDSFDIQLTSVQLLYSRVGDNWREARKLSVSTQHILVPMHFNLELSKAMVFMDVRMPKFKIYGKLPLISLRISDKKLQGIMELIESIPKPEPVTEVSAPVKSFQIQTSTSLGTSQISQKIIPLLELPSVSEDDSEEEFFDAPCSPLEEPLQFPTGVKSIRTRKLQKQDCSV--------------NMTTFKIRFEVPKVLIEFYHLVGDCELS------VVEILVLGLGAEIEIRTYDLKANAFLKEFCLKCPEYLD-ENKKPVYLVTTLDNTM-EDLLTLEYVKAEKNVPDLKSTYNNVLQLIKVNFSSLDIHLHTEALLNTINYLHNILPQ----SEEKSAPVST---------TETEDKGDVIKKLA---------------LKLSTNEDIITLQ------------------------------ILAELSCLQIFIQDQK-CNISEIKIEGLDSEMIMRPSETEINAKLRNIIVLDSDITAIYKKAVYITGKE-VFSFKMVSYMDATAGSAYT-DMNVVDIQVNLIVGCIEVVFVTKFLYSILAFIDNFQAAKQALAEATVQAAGMAATGVKELAQR--SSRMALDINIKAPVVVIPQSPVSENVFVADFGLITMTNTFHMITESQSSPPPVIDLITIKLSEMRLYRSRFINDAYQEVLDLLLPLNLEVVVERNLCWEWYQEVPCFNVNAQLKPMEFILSQEDITTIFKTLHGNIWYEKDGSASPAVTKDQYSATSGVTTNASHHSGGATVVTAAVVEVHSRALLVKTTLNISFKTDDLTMVLYSPGPKQASFTDVRDPSLKLAEFKLENIISTLKMYTDGSTFSSFSLKNC-ILDDKRPHVKKATPRMIGLTVGFDKKDMMDIKYRKVRDGCVTDAV-FQEMYICASVEFLQTVANVFLEAYTTGT-----------AVETSVQTWTAKEEVPTQES--VKWEINVIIKNPEIVFVADMTKNDAPALVITTQCEICYKGNLENSTMTAAIKDLQVRACPFLPVKRKGKITTVLQPCDLFYQTTQKGTDPQVIDMSVKSLTLKVSPVIINTMITITSAL--YTTKET-------IPEETASSTAHLWEKKDTKTLKMWFLEES-NETEKIAPTTELVPKG------------------------------EMIKMNIDSIFIVLEAGIGHRTVPMLLAKSRFSGEGKNWSSLINLHCQLELEVHYYNEMFGVWEPLLEPLEIDQTEDFRPWNLGIKMKKKAKMAIVESDPEEENYKVPEYKTVISFHSKDQLNITLSKCGLVMLNNLVKAFTEAATGSSADFVKDL-APFMILNSLGLTISVSPSDSFSVLNIPMA---KSYVLKNGESLSMDYIRTKDN----------DHFNAMTSLSSKLFFILLTPVNHSTADKIPLTKVGRRLYTVRHRESGVERSIVCQIDTVEGSKKVTIRSPVQIRNHFSVPLSVY------------EGDTLLGTASPENEFNIPLGSYRSFIFLKPEDENYQMCEGIDFEEIIKNDGALLKKKCRSKNPSKESFLINIVPEKDNLTSLSVYS------------EDGWDLPYIMHLWPPILLRNLLPYKIAYYIEGIENSVFT--LSEGHSAQICTAQLGKARLHLKL-LDYLNHDWKSEYHIKPNQQDISFVSFTCVTEMEKTDLDIAVHMTYNTGQTVVAFHSPYWMVN--------KTGRMLQYKADG--------------------------IHRKHPPNYKKPVLFSFQP-----------------NHFFNNN-------KVQLMVTDSELSNQFSIDTVGSHGAVKCKGLKMDYQVGVTIDLSSFNITRIVTFTPFYMIKNKSKYHISVAE-EGNDKWLSLDLEQCIPFWPEYASSKLLIQVERSED------PPKRIYFNKQENCILLRLDNELGGIIAEVNLAEHSTVITFLDYHDGAATFLLINHTKNEL-VQYNQSSLSEIEDSLPPGKAVFYTWADPVGSRRLKWRC---RKSHGEVTQKDDMMMPIDLGEKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAEQEIAVALQDVGISLVNNYTKQEVAYIGITSSDVVWETKPKKKARWKPMSVKHTEKLEREFKEY-----TESSPSEDKVIQLDTNVPVRLTPTGHNMKILQPHVIALRRNYLPALKVEYNTSAHQSSFRIQIYRIQIQNQIHGAVFPFVFYPVKPPKSVTMDSAPKPFTDVSIVMRSAGHSQISRIKYFKVLIQEMDLRLDLGFIYALTDLMTEAEVTENTEVELFHKDIEAFKEEYKTASLVDQSQV---------SLYEYFHISPIKLHLSVSLSSGREEAKDSKQNGGLIPVHS--LNLLLKSIGATLTDVQDVVFKLAFFELNYQFHTTSDLQSEVIRHYSKQAIKQMYVLILGLDVLGNPFGLIREFSEGVEAFFYEPYQGAIQGPEEFVEGMALGLKALVGGAVGGLAGAASKITGAMAKGVAAMTMDEDYQQKRREAMNKQPAGFREGITRGGKGLVSGFVSGITGIVTKPIKGAQKGGAAGFFKGVGKGLVGAVARPTGGIIDMASSTFQGIKRATETS-EVESLRPPRFFNEDGVIRPYRLRDGTGNQMLQKI 3065          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Human
Match: VPS13A (vacuolar protein sorting 13 homolog A [Source:HGNC Symbol;Acc:HGNC:1908])

HSP 1 Score: 1221.07 bits (3158), Expect = 0.000e+0
Identity = 1010/3354 (30.11%), Postives = 1629/3354 (48.57%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKE-TETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSS-------SLASDGSDELQKKVRSE-----FTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGS-VPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSE-RTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQ-----TAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSN--FNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDE-----------SITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELI--RNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLE---VYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFS--PKDNEGPSRGNVSEREIN---TLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPT-----LLTSNGKIYISVKQLNTE--ISNSGALWNIG-KNAQPSDTQILLC----KSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISY-----QSQIFSHDIKPGCLELVRTACL-KVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQSLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFY---ESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQ-ELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSN-NLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKW-NQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVE-GLEVDLSKK----LMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEA-MFVKDCQMIR-----ASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA--------LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNI 9815
            MVFES+VV+++N++LGDYVV LD SQL LG+  G   L+NL IKENA    D+P KV+ GHI  L L IPWK+LY++PV A L+ +Y+LI+P+S +KYDP KE   L E KQ+EL RIEE K K  D ++   E +D+F EKL  QI+KNLQ+ IS+IHIRYED  T  ++  + GI+L NLS QT D      +  +++ +   K +++D+L  YWN   + F   +       +KN I +         F+ +PIS +A + ++ + +  +F+ P+++L+++L  I   FN+ QY  I   L+S+D       +RK++P+VP+  +   WW +  + +LE  V  R  MWSWK+I+ HR + + Y +++++KL  KK       +++  E+ LD+ NIT+ RQ AE++ KK   K+ ++              D E +        S +   ++E Q  V+ E      T EEKA LY+AIGYSE   DP +   + +LK  + L+ +  ++ +N  K  +L+ +VI   S  + QR  A AI     +++  +TG   PD S  P L+S+   +  M+L  + FE NPLD++ +Q+  I AEPLEIIYD+ TVN IV+F  PP+E+ L +++   L  +EE +  TATGL ++ + +   D+K+++K SY I+P+ G ++    +LL+DLG + + S+ R+ +P          K G+ N   + + AYD F I L+++Q L    GD+WR+ R    S  HIL P  F   L K ++  D  +PK  + G LP + + ISD+ LQ ++ELI  IP P+     +AP K      S     S     I P L L S     +     D+  + L   +             L+  D +V              NM      F +    ++    + +  +       +V+++V  +G  + ++ +D+   A++    L  P+++D   K PV L  T  +T  ++LL +  +  EK  PD  + + N    ++  F S+++ LH EALL  +N++++++P      EEK+   S+         T  E + + I+ L                + L+T +  + L+                              I A L  L++ + ++K  NI +I I G+ +++      TE++  + ++ + D    A+YKK ++I   E +FS ++  +   T+   Y  D++ VD  VN+ VG I+++F+  F+  I  F+++FQ + Q +A    + +  AA  +  + +   SSR  LDIN+KAPV+ IP++  S N  + D G +T  N F +      S   V++   I LS +R+ RS  + +  +    +L P+N+ + + RNL W     +P  ++ A++  +   +SQ D       L  N  + +  +  P+V + +    S         SG A   +               T+   FK + L + L+        +     P   L + K EN+     M+ + +   S  L N  +LD +    KK    +    +  D  D + IK+ + ++      V    +Y+C  V++L  + + F++A                  E + + +  K      E     +EI+V +  PEI+ V DM   D+ A+++ T   + Y    E+  +   I D Q   CPF    RK     ++ P  +     +   +     ++++ L + ++P +I ++  I + L   +T ET       I  +      + WEK++    +  F+ +   E EKI    + V  G                                I + +  I ++LE+ +  + VPML+ +    G+ KN  + I L   + + V Y+N+   VWEPLLE   +   E  R ++  I+        + + +  E+   +      I   S+D L +T+SK  L +L+NL KAF +A +           AP+++ N+ G  +    +  F +         K Y+  +GE    D +  K +            F    S+S +   I  T V        P    G     V  RE     +++       G K++ +RS +Q+ N+    +++Y            +   LL        +N+P+ +      L    + Y   + ++ E  I N GAL     ++  PS    L+     K N   +  YS                   Y + +  P+ L N LP KI+Y     ++ +F+  +  G    + TA L K + ++K+   Y   +W     + P  +++S ++F       ++ +D+ VH+    GQ  V  +SPYWM+N        K G   Q + D                           I +K    Y    L + +                  N+  N N       K  L + DS  S++FS+DTVGS G V CK   + Y+VGV I+LSS  +T+IVTF P+YMI N+S   +   E   N  W+ +   + +PFWP     K ++   + ED       P   Y   + + IL++L+ +  GI  EV + E + +I    Y +G A   LINH  +++ + + Q +  + E  LP  + V Y W D    R   WR    +K H       D +    + E   Y VSF     RI+LFT D  V K    S   E  +QEI ++L  +G+SLV+N  +QE+AYI ITSS V W  + ++K R KP+ +  +E LE  F E+     T  +  E+ +++++  + V L+       +++P    L R + P + ++Y TS HQ    ++IY+IQI NQ+    FP VF P  PP+SV +D+  KP  ++S V+  +G +   R KYF+VLIQEM L LD GF+ ++ D ++ +  T + E  +F KD +  +     ASL++ S +          +++ FHISPIK+HLS SLS      K +        +HS  LNL L+S+G  +TD+QDV+FKL +FE        + + S +  HY  QA++QMYVL+LGLDVLGNPFG+IR  ++GVE  FYEP +GA+ GPEEFVEG+A G+K+L G AVGG AGA S+I G M KG+AA+T+D DYQ+KRRE + K+P     G+ +GGKGL+ GFV G+TG+VTKPI GA+K G  GFFKGVGKGLVG V RP  G++D ASS+F+GI+R  +T+ EV  +RPPRF  +DG+IR Y LR+  GN +
Sbjct:    1 MVFESVVVDVLNRFLGDYVVDLDTSQLSLGIWKGAVALKNLQIKENALSQLDVPFKVKVGHIGNLKLIIPWKNLYTQPVEAVLEEIYLLIVPSSRIKYDPLKEEKQLMEAKQQELKRIEEAKQKVVDQEQHLPEKQDTFAEKLVTQIIKNLQVKISSIHIRYEDDITNRDKPLSFGISLQNLSMQTTDQYWVPCL-HDETEKLVRKLIRLDNLFAYWNVKSQMFYLSDYDNSLDDLKNGIVNENIVPEGYDFVFRPISANAKLVMNRRSDF-DFSAPKINLEIELHNIAIEFNKPQYFSIMELLESVDMMAQNLPYRKFKPDVPLHHHAREWWAYAIHGVLEVNVCPRLWMWSWKHIRKHRQKVKQYKELYKKKLTSKKPPGELLVSLEELEKTLDVFNITIARQTAEVEVKKAGYKIYKEGVK-----------DPEDNKGWFSWLWSWSEQNTNEQQPDVQPETLEEMLTPEEKALLYEAIGYSETAVDPTLLKTFEALKFFVHLKSMSIVLRENHQKP-ELVDIVIEEFSTLIVQRPGAQAIKFETKIDSFHITGL--PDNSEKPRLLSSLDDA--MSLFQITFEINPLDETVSQRCIIEAEPLEIIYDARTVNSIVEFFRPPKEVHLAQLTAATLTKLEEFRSKTATGLLYIIETQKVLDLKINLKASYIIVPQDGIFSPTSNLLLLDLGHLKVTSKSRSELPDV--------KQGEANLKEIMDRAYDSFDIQLTSVQLLYSRVGDNWREARKLSVSTQHILVPMHFNLELSKAMVFMDVRMPKFKIYGKLPLISLRISDKKLQGIMELIESIPKPEPVTEVSAPVKSFQIQTSTSLGTSQISQKIIPLLELPSVSEDDSEEEFFDAPCSPLEEPLQFPTGVKSIRTRKLQKQDCSV--------------NMTTFKIRFEVPKVLIEFYHLVGDCELS------VVEILVLGLGAEIEIRTYDLKANAFLKEFCLKCPEYLD-ENKKPVYLVTTLDNTM-EDLLTLEYVKAEKNVPDLKSTYNNVLQLIKVNFSSLDIHLHTEALLNTINYLHNILPQ----SEEKSAPVST---------TETEDKGDVIKKLA---------------LKLSTNEDIITLQ------------------------------ILAELSCLQIFIQDQK-CNISEIKIEGLDSEMIMRPSETEINAKLRNIIVLDSDITAIYKKAVYITGKE-VFSFKMVSYMDATAGSAYT-DMNVVDIQVNLIVGCIEVVFVTKFLYSILAFIDNFQAAKQALAEATVQAAGMAATGVKELAQR--SSRMALDINIKAPVVVIPQSPVSENVFVADFGLITMTNTFHMITESQSSPPPVIDLITIKLSEMRLYRSRFINDAYQEVLDLLLPLNLEVVVERNLCWEWYQEVPCFNVNAQLKPMEFILSQEDITTIFKTLHGNIWYEKDGSASPAVTKDQYSATSGVTTNASHHSGGATVVTAAVVEVHSRALLVKTTLNISFKTDDLTMVLYSPGPKQASFTDVRDPSLKLAEFKLENIISTLKMYTDGSTFSSFSLKNC-ILDDKRPHVKKATPRMIGLTVGFDKKDMMDIKYRKVRDGCVTDAV-FQEMYICASVEFLQTVANVFLEAYTTGT-----------AVETSVQTWTAKEEVPTQES--VKWEINVIIKNPEIVFVADMTKNDAPALVITTQCEICYKGNLENSTMTAAIKDLQVRACPFLPVKRKGKITTVLQPCDLFYQTTQKGTDPQVIDMSVKSLTLKVSPVIINTMITITSAL--YTTKET-------IPEETASSTAHLWEKKDTKTLKMWFLEES-NETEKIAPTTELVPKG------------------------------EMIKMNIDSIFIVLEAGIGHRTVPMLLAKSRFSGEGKNWSSLINLHCQLELEVHYYNEMFGVWEPLLEPLEIDQTEDFRPWNLGIKMKKKAKMAIVESDPEEENYKVPEYKTVISFHSKDQLNITLSKCGLVMLNNLVKAFTEAATGSSADFVKDL-APFMILNSLGLTISVSPSDSFSVLNIPMA---KSYVLKNGESLSMDYIRTKDN----------DHFNAMTSLSSKLFFILLTPVNHSTADKIPLTKVGRRLYTVRHRESGVERSIVCQIDTVEGSKKVTIRSPVQIRNHFSVPLSVY------------EGDTLLGTASPENEFNIPLGSYRSFIFLKPEDENYQMCEGIDFEEIIKNDGALLKKKCRSKNPSKESFLINIVPEKDNLTSLSVYS------------EDGWDLPYIMHLWPPILLRNLLPYKIAYYIEGIENSVFT--LSEGHSAQICTAQLGKARLHLKL-LDYLNHDWKSEYHIKPNQQDISFVSFTCVTEMEKTDLDIAVHMTYNTGQTVVAFHSPYWMVN--------KTGRMLQYKADG--------------------------IHRKHPPNYKKPVLFSFQP-----------------NHFFNNN-------KVQLMVTDSELSNQFSIDTVGSHGAVKCKGLKMDYQVGVTIDLSSFNITRIVTFTPFYMIKNKSKYHISVAE-EGNDKWLSLDLEQCIPFWPEYASSKLLIQVERSED------PPKRIYFNKQENCILLRLDNELGGIIAEVNLAEHSTVITFLDYHDGAATFLLINHTKNEL-VQYNQSSLSEIEDSLPPGKAVFYTWADPVGSRRLKWRC---RKSHGEVTQKDDMMMPIDLGEKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAEQEIAVALQDVGISLVNNYTKQEVAYIGITSSDVVWETKPKKKARWKPMSVKHTEKLEREFKEY-----TESSPSEDKVIQLDTNVPVRLTPTGHNMKILQPHVIALRRNYLPALKVEYNTSAHQSSFRIQIYRIQIQNQIHGAVFPFVFYPVKPPKSVTMDSAPKPFTDVSIVMRSAGHSQISRIKYFKVLIQEMDLRLDLGFIYALTDLMTEAEVTENTEVELFHKDIEAFKEEYKTASLVDQSQV---------SLYEYFHISPIKLHLSVSLSSGREEAKDSKQNGGLIPVHS--LNLLLKSIGATLTDVQDVVFKLAFFELNYQFHTTSDLQSEVIRHYSKQAIKQMYVLILGLDVLGNPFGLIREFSEGVEAFFYEPYQGAIQGPEEFVEGMALGLKALVGGAVGGLAGAASKITGAMAKGVAAMTMDEDYQQKRREAMNKQPAGFREGITRGGKGLVSGFVSGITGIVTKPIKGAQKGGAAGFFKGVGKGLVGAVARPTGGIIDMASSTFQGIKRATETS-EVESLRPPRFFNEDGVIRPYRLRDGTGNQM 3061          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Human
Match: VPS13A (vacuolar protein sorting 13 homolog A [Source:HGNC Symbol;Acc:HGNC:1908])

HSP 1 Score: 697.197 bits (1798), Expect = 0.000e+0
Identity = 649/2250 (28.84%), Postives = 1054/2250 (46.84%), Query Frame = 3
Query: 3555 TEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSN--FNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDE-----------SITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELI--RNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLE---VYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFS--PKDNEGPSRGNVSEREIN---TLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPT-----LLTSNGKIYISVKQLNTE--ISNSGALWNIG-KNAQPSDTQILLC----KSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISY-----QSQIFSHDIKPGCLELVRTACL-KVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQSLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFY---ESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQ-ELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSN-NLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKW-NQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVE-GLEVDLSKK----LMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEA-MFVKDCQMIR-----ASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA--------LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEI 10094
            TE++  + ++ + D    A+YKK ++I   E +FS ++  +   T+   Y  D++ VD  VN+ VG I+++F+  F+  I  F+++FQ + Q +A    + +  AA  +  + +   SSR  LDIN+KAPV+ IP++  S N  + D G +T  N F +      S   V++   I LS +R+ RS  + +  +    +L P+N+ + + RNL W     +P  ++ A++  +   +SQ D       L  N  + +  +  P+V + +    S         SG A   +               T+   FK + L + L+        +     P   L + K EN+     M+ + +   S  L N  +LD +    KK    +    +  D  D + IK+ + ++      V    +Y+C  V++L  + + F++A                  E + + +  K      E     +EI+V +  PEI+ V DM   D+ A+++ T   + Y    E+  +   I D Q   CPF    RK     ++ P  +     +   +     ++++ L + ++P +I ++  I + L   +T ET       I  +      + WEK++    +  F+ +   E EKI    + V  G                                I + +  I ++LE+ +  + VPML+ +    G+ KN  + I L   + + V Y+N+   VWEPLLE   +   E  R ++  I+        + + +  E+   +      I   S+D L +T+SK  L +L+NL KAF +A +           AP+++ N+ G  +    +  F +         K Y+  +GE    D +  K +            F    S+S +   I  T V        P    G     V  RE     +++       G K++ +RS +Q+ N+    +++Y            +   LL        +N+P+ +      L    + Y   + ++ E  I N GAL     ++  PS    L+     K N   +  YS                   Y + +  P+ L N LP KI+Y     ++ +F+  +  G    + TA L K + ++K+   Y   +W     + P  +++S ++F       ++ +D+ VH+    GQ  V  +SPYWM+N        K G   Q + D                           I +K    Y    L + +                  N+  N N       K  L + DS  S++FS+DTVGS G V CK   + Y+VGV I+LSS  +T+IVTF P+YMI N+S   +   E   N  W+ +   + +PFWP     K ++   + ED       P   Y   + + IL++L+ +  GI  EV + E + +I    Y +G A   LINH  +++ + + Q +  + E  LP  + V Y W D    R   WR    +K H       D +    + E   Y VSF     RI+LFT D  V K    S   E  +QEI ++L  +G+SLV+N  +QE+AYI ITSS V W  + ++K R KP+ +  +E LE  F E+     T  +  E+ +++++  + V L+       +++P    L R + P + ++Y TS HQ    ++IY+IQI NQ+    FP VF P  PP+SV +D+  KP  ++S V+  +G +   R KYF+VLIQEM L LD GF+ ++ D ++ +  T + E  +F KD +  +     ASL++ S +          +++ FHISPIK+HLS SLS      K +        +HS  LNL L+S+G  +TD+QDV+FKL +FE        + + S +  HY  QA++QMYVL+LGLDVLGNPFG+IR  ++GVE  FYEP +GA+ GPEEFVEG+A G+K+L G AVGG AGA S+I G M KG+AA+T+D DYQ+KRRE + K+P     G+ +GGKGL+ GFV G+TG+VTKPI GA+K G  GFFKGVGKGLVG V RP  G++D ASS+F+GI+R  +T+ EV  +RPPRF  +DG+IR Y LR+  GN + +++ E         Y  HV   +K +++ +T + +  + +    G  + +WQ ++++    P I   +  +    KE+ + +F      K I
Sbjct: 1052 TEINAKLRNIIVLDSDITAIYKKAVYITGKE-VFSFKMVSYMDATAGSAYT-DMNVVDIQVNLIVGCIEVVFVTKFLYSILAFIDNFQAAKQALAEATVQAAGMAATGVKELAQR--SSRMALDINIKAPVVVIPQSPVSENVFVADFGLITMTNTFHMITESQSSPPPVIDLITIKLSEMRLYRSRFINDAYQEVLDLLLPLNLEVVVERNLCWEWYQEVPCFNVNAQLKPMEFILSQEDITTIFKTLHGNIWYEKDGSASPAVTKDQYSATSGVTTNASHHSGGATVVTAAVVEVHSRALLVKTTLNISFKTDDLTMVLYSPGPKQASFTDVRDPSLKLAEFKLENIISTLKMYTDGSTFSSFSLKNC-ILDDKRPHVKKATPRMIGLTVGFDKKDMMDIKYRKVRDGCVTDAV-FQEMYICASVEFLQTVANVFLEAYTTGT-----------AVETSVQTWTAKEEVPTQES--VKWEINVIIKNPEIVFVADMTKNDAPALVITTQCEICYKGNLENSTMTAAIKDLQVRACPFLPVKRKGKITTVLQPCDLFYQTTQKGTDPQVIDMSVKSLTLKVSPVIINTMITITSAL--YTTKET-------IPEETASSTAHLWEKKDTKTLKMWFLEES-NETEKIAPTTELVPKG------------------------------EMIKMNIDSIFIVLEAGIGHRTVPMLLAKSRFSGEGKNWSSLINLHCQLELEVHYYNEMFGVWEPLLEPLEIDQTEDFRPWNLGIKMKKKAKMAIVESDPEEENYKVPEYKTVISFHSKDQLNITLSKCGLVMLNNLVKAFTEAATGSSADFVKDL-APFMILNSLGLTISVSPSDSFSVLNIPMA---KSYVLKNGESLSMDYIRTKDN----------DHFNAMTSLSSKLFFILLTPVNHSTADKIPLTKVGRRLYTVRHRESGVERSIVCQIDTVEGSKKVTIRSPVQIRNHFSVPLSVY------------EGDTLLGTASPENEFNIPLGSYRSFIFLKPEDENYQMCEGIDFEEIIKNDGALLKKKCRSKNPSKESFLINIVPEKDNLTSLSVYS------------EDGWDLPYIMHLWPPILLRNLLPYKIAYYIEGIENSVFT--LSEGHSAQICTAQLGKARLHLKL-LDYLNHDWKSEYHIKPNQQDISFVSFTCVTEMEKTDLDIAVHMTYNTGQTVVAFHSPYWMVN--------KTGRMLQYKADG--------------------------IHRKHPPNYKKPVLFSFQP-----------------NHFFNNN-------KVQLMVTDSELSNQFSIDTVGSHGAVKCKGLKMDYQVGVTIDLSSFNITRIVTFTPFYMIKNKSKYHISVAE-EGNDKWLSLDLEQCIPFWPEYASSKLLIQVERSED------PPKRIYFNKQENCILLRLDNELGGIIAEVNLAEHSTVITFLDYHDGAATFLLINHTKNEL-VQYNQSSLSEIEDSLPPGKAVFYTWADPVGSRRLKWRC---RKSHGEVTQKDDMMMPIDLGEKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAEQEIAVALQDVGISLVNNYTKQEVAYIGITSSDVVWETKPKKKARWKPMSVKHTEKLEREFKEY-----TESSPSEDKVIQLDTNVPVRLTPTGHNMKILQPHVIALRRNYLPALKVEYNTSAHQSSFRIQIYRIQIQNQIHGAVFPFVFYPVKPPKSVTMDSAPKPFTDVSIVMRSAGHSQISRIKYFKVLIQEMDLRLDLGFIYALTDLMTEAEVTENTEVELFHKDIEAFKEEYKTASLVDQSQV---------SLYEYFHISPIKLHLSVSLSSGREEAKDSKQNGGLIPVHS--LNLLLKSIGATLTDVQDVVFKLAFFELNYQFHTTSDLQSEVIRHYSKQAIKQMYVLILGLDVLGNPFGLIREFSEGVEAFFYEPYQGAIQGPEEFVEGMALGLKALVGGAVGGLAGAASKITGAMAKGVAAMTMDEDYQQKRREAMNKQPAGFREGITRGGKGLVSGFVSGITGIVTKPIKGAQKGGAAGFFKGVGKGLVGAVARPTGGIIDMASSTFQGIKRATETS-EVESLRPPRFFNEDGVIRPYRLRDGTGNQM-LQVMENGR-FAKYKYFTHVMI-NKTDMLMITRRGVLFVTKG-TFGQLTCEWQYSFDEFTKEPFIVHGRR-LRIEAKERVKSVFHAREFGKII 3110          

HSP 2 Score: 549.666 bits (1415), Expect = 2.978e-156
Identity = 358/1064 (33.65%), Postives = 572/1064 (53.76%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKE-TETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFF-KSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSS-------SLASDGSDELQKKVRSE-----FTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGS-VPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSE-RTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQ-----TAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIP 3152
            MVFES+VV+++N++LGDYVV LD SQL LG+  G   L+NL IKENA    D+P KV+ GHI  L L IPWK+LY++PV A L+ +Y+LI+P+S +KYDP KE   L E KQ+EL RIEE K K  D ++   E +D+F EKL  QI+KNLQ+ IS+IHIRYED  T  ++  + GI+L NLS QT D      +   D T +   K +++D+L  YWN   + F   +       +KN I +         F+ +PIS +A + ++ + +  +F+ P+++L+++L  I   FN+ QY  I   L+S+D       +RK++P+VP+  +   WW +  + +LE  V  R  MWSWK+I+ HR + + Y +++++KL  KK       +++  E+ LD+ NIT+ RQ AE++ KK   K+ ++              D E +        S +   ++E Q  V+ E      T EEKA LY+AIGYSE   DP +   + +LK  + L+ +  ++ +N  K  +L+ +VI   S  + QR  A AI     +++  +TG   PD S  P L+S+   +  M+L  + FE NPLD++ +Q+  I AEPLEIIYD+ TVN IV+F  PP+E+ L +++   L  +EE +  TATGL ++ + +   D+K+++K SY I+P+ G ++    +LL+DLG + + S+ R+ +P          K G+ N   + + AYD F I L+++Q L    GD+WR+ R    S  HIL P  F   L K ++  D  +PK  + G LP + + ISD+ LQ ++ELI  IP P+     +AP K      S     S     I P L L S     +     D+  + L   +             L+  D +V              NM      F +    ++    + +  +       +V+++V  +G  + ++ +D+   A++    L  P+++D   K PV L  T  +T  ++LL +  +  EK  PD  + + N    ++  F S+++ LH EALL  +N++++++P
Sbjct:    1 MVFESVVVDVLNRFLGDYVVDLDTSQLSLGIWKGAVALKNLQIKENALSQLDVPFKVKVGHIGNLKLIIPWKNLYTQPVEAVLEEIYLLIVPSSRIKYDPLKEEKQLMEAKQQELKRIEEAKQKVVDQEQHLPEKQDTFAEKLVTQIIKNLQVKISSIHIRYEDDITNRDKPLSFGISLQNLSMQTTDQYWVPCL--HDETEKLVRKLIRLDNLFAYWNVKSQMFYLSDYDNSLDDLKNGIVNENIVPEGYDFVFRPISANAKLVMNRRSD-FDFSAPKINLEIELHNIAIEFNKPQYFSIMELLESVDMMAQNLPYRKFKPDVPLHHHAREWWAYAIHGVLEVNVCPRLWMWSWKHIRKHRQKVKQYKELYKKKLTSKKPPGELLVSLEELEKTLDVFNITIARQTAEVEVKKAGYKIYKEG-----------VKDPEDNKGWFSWLWSWSEQNTNEQQPDVQPETLEEMLTPEEKALLYEAIGYSETAVDPTLLKTFEALKFFVHLKSMSIVLRENHQKP-ELVDIVIEEFSTLIVQRPGAQAIKFETKIDSFHITGL--PDNSEKPRLLSSLDDA--MSLFQITFEINPLDETVSQRCIIEAEPLEIIYDARTVNSIVEFFRPPKEVHLAQLTAATLTKLEEFRSKTATGLLYIIETQKVLDLKINLKASYIIVPQDGIFSPTSNLLLLDLGHLKVTSKSRSELPDV--------KQGEANLKEIMDRAYDSFDIQLTSVQLLYSRVGDNWREARKLSVSTQHILVPMHFNLELSKAMVFMDVRMPKFKIYGKLPLISLRISDKKLQGIMELIESIPKPEPVTEVSAPVKSFQIQTSTSLGTSQISQKIIPLLELPSVSEDDSEEEFFDAPCSPLEEPLQFPTGVKSIRTRKLQKQDCSV--------------NMTTFKIRFEVPKVLIEFYHLVGDCEL------SVVEILVLGLGAEIEIRTYDLKANAFLKEFCLKCPEYLD-ENKKPVYLVTTLDNTM-EDLLTLEYVKAEKNVPDLKSTYNNVLQLIKVNFSSLDIHLHTEALLNTINYLHNILP 1015          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Human
Match: VPS13C (vacuolar protein sorting 13 homolog C [Source:HGNC Symbol;Acc:HGNC:23594])

HSP 1 Score: 645.966 bits (1665), Expect = 0.000e+0
Identity = 443/1457 (30.40%), Postives = 752/1457 (51.61%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEE--NKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSE--TASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIP----VLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQV----PSVPESKE 4346
            MV ES+V +L+N++LGDYV  L+KSQLKLG+ GGN  L+NL IKENA  + D+P KV+ G I KLTLKIPWK+LY E V+A L+GLY+L++P + +KYD  KE   L + KQKEL+RIEE   K    D  KE + KD+F EKLA Q++KN+Q+ I++IHI+YED  T P R  + G+TL  LS  T +   T  I+ E + +  +K +++DSL+ YWN N       +R  +   +KN I +S     +  ++ QPIS SA ++++   E +    P++  +++++ I     + QY  +   L+S+D       +RKY+P +P+ +N   WW +  + +LE  +RR  +MWSW  I+ HR   + Y   ++ KL   K+S+  Q  I+  E+ LD+ NI L RQQA+++  +  +KL +K++  G   G +         S   D    + + +    T EEK KL+ AIGYSE T +  +P +YV+  + L+L      I +N     +++K+ I  +   V QR  A A+ V   + +  +TG +  D  VP+LV++   +   +LL + FETNP D  A+Q + ++++P+E+IYD+ TVN +V+F    + L L +I++  L  +EE+KE TATGL H+ + R   D+++++KPSY ++P+ G + +   +L++D G+  + S+   +  + ++ L          + + + AYDKF + +  +Q L     + W+K R Q  S  HIL+P      L K ++  D  + +  V G LP + V ISD+ ++ VL L+  IP PQ +  +      S +   SG   G+     L ++ +L  +    D E   A           +  E K A E+ +                  +I+L   F I    L+  +  K           I+   V ++GT  T++ FD+ V +Y+  + L   + ++ S + P+ L  +     G +LL +  +  +K  P F T    T   ++  F S+ + L  +AL+  +N++ ++IP     +++ +E +  T      E+ +K +TL K   +  D              +D ID                            ++F            A L    VIV NEK NNI +I I G+ + +        +   + ++ + D  P  V+KK + I  +E +F   + L+   T    Y  D+S+VD ++++ VG I+I++L+ F++ +  FLN+FQ + ++++    + +E AA  + ++ +   S R  ++I++KAPVI IP++S S NA++VDLG +   N F++    D     V++   + L+ L + R+++       +  +LHPIN+   + RNL+ +  H +P + I   + S++V ++Q D N+   +L  N  E    +    P V E+ E
Sbjct:    1 MVLESVVADLLNRFLGDYVENLNKSQLKLGIWGGNVALDNLQIKENALSELDVPFKVKAGQIDKLTLKIPWKNLYGEAVVATLEGLYLLVVPGASIKYDAVKEEKSLQDVKQKELSRIEEALQKAAEKDKPKEAK-KDTFVEKLATQVIKNVQVKITDIHIKYEDDVTDPKRPLSFGVTLGELSLLTANEHWTPCILNE-ADKIIYKLIRLDSLSAYWNVNCSMSYQRSREQILDQLKNEILTSGNIPPNYQYIFQPISASAKLYMNPYAE-SELKTPKLDCNIEIQNIAIELTKPQYLSMIDLLESVDYMVRNAPYRKYKPYLPLHTNGRRWWKYAIDSVLEVHIRRYTQMWSWSNIKKHRQLLKSYKIAYKNKLTQSKVSEEIQKEIQDLEKTLDVFNIILARQQAQVEVIRSGQKLRKKSADTGEKRGGWFSGLWGKKESKKKDEESLIPETIDDLMTPEEKDKLFTAIGYSESTHNLTLPKQYVAHIMTLKLVSTSVTIRENK-NIPEILKIQIIGLGTQVSQRPGAQALKVEAKLEHWYITGLRQQD-IVPSLVASIGDTT-SSLLKIKFETNPEDSPADQTLIVQSQPVEVIYDAKTVNAVVEFFQSNKGLDLEQITSATLMKLEEIKERTATGLTHIIETRKVLDLRINLKPSYLVVPQTGFHHEKSDLLILDFGTFQLNSKDQGLQKTTNSSL----------EEIMDKAYDKFDVEIKNVQLLFARAEETWKKCRFQHPSTMHILQPMDIHVELAKAMVEKDIRMARFKVSGGLPLMHVRISDQKMKDVLYLMNSIPLPQKSSAQSPERQVSSIPIISGGTKGL-----LGTSLLLDTVESESDDEYFDAEDGEPQTCKSMKGSELKKAAEVPNE----------------ELINLLLKFEIKEVILEFTKQQKE-------EDTILVFNVTQLGTEATMRTFDLTVVSYLKKISLDYHE-IEGSKRKPLHLISSSDKP-GLDLLKVEYIKADKNGPSFQTAFGKTEQTVKVAFSSLNLLLQTQALVASINYLTTIIPSDDQSISVAKEVQIST------EKQQKNSTLPKAIVSSRD--------------SDIIDF---------------------------RLF------------AKLNAFCVIVCNEK-NNIAEIKIQGLDSSLSLQSRKQSLFARLENIIVTDVDPKTVHKKAVSIMGNE-VFRFNLDLYPDATEGDLYT-DMSKVDGVLSLNVGCIQIVYLHKFLMSLLNFLNNFQTAKESLSAATAQAAERAATSVKDLAQR--SFRVSINIDLKAPVIVIPQSSISTNAVVVDLGLIRVHNQFSLVSDEDYLNPPVIDRMDVQLTKLTLYRTVIQPGIYHPDIQLLHPINLEFLVNRNLAASWYHKVPVVEIKGHLDSMNVSLNQEDLNLLFRILTENLCEGTEDLDKVKPRVQETGE 1347          

HSP 2 Score: 515.768 bits (1327), Expect = 9.921e-146
Identity = 359/831 (43.20%), Postives = 517/831 (62.21%), Query Frame = 3
Query: 7395 KAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG-----WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFY--ESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDF-YINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEG-LEVDLSKKLMIK--PEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEA------MFVKDCQMIRASLIETS-TIKTILSGGKKCVFDNFHISPIKIHLSFSL------SRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNI 9815
            K  LKI+ S WS  FSLDTVGS G V C    + Y VGV I +SS  L++IVT  P+  I N+SS+ L   E+  +       W  +   E +PFWP S  GK  +     E      ++PF FY  + +  L+ LE    GI V+V   E + +I    Y EG A   ++NH   D+ + +KQ  +P+E++L   Q  ++AW D    R+  W    +  +H  +L  DG   F Y   ++ +WVSF+    R+LLFT+D+ +   A  + ++E  D EIT+SLHS+GLSLV+N  +QE++YI ITSSGV W +++ K + KP    Q   LE ++       +  +   +   +K++   EV+  K  M    P +  + R F  G+ ++++ S HQ  +  R+Y +Q+DNQ+    FPVVF P  PP+S+ALD+  KP I++S +   +  +  +++KYF VLIQEM L +DQGF+ +I+   +   PT D EA      +  +D   + A L+ETS T  +ILS      F++FHISP+K+HLS SL      S   +    A+HS  +NL L+S+G  +TD+ D+IFKL Y+E +      +Q++ S+  HY  Q L+QMYVLVLGLDVLGNPFG+IRG+++GVE LFYEP +GAV GPEEF EG+  GV+SLFGH VGGAAG VSRI G++GKG+AA+T+D +YQ+KRRE+L+++P + G  LA+GGKG + G V GVTG++TKP++GAKK+G  GFFKG+GKGLVG V RP  G+VD ASS+F+GI+R A++  EV  +RPPR I +DGIIR Y  +E+EG+++
Sbjct: 2772 KVQLKISTSAWSSSFSLDTVGSYGCVKCPANNMEYLVGVSIKMSSFNLSRIVTLTPFCTIANKSSLELEVGEIASDGSMPTNKWNYIASSECLPFWPESLSGKLCVRVVGCEGS----SKPF-FYNRQDNGTLLSLEDLNGGILVDVNTAEHSTVITFSDYHEGSAPALIMNHTPWDI-LTYKQSGSPEEMVLLPRQARLFAWADPTGTRKLTWTYAANVGEH--DLLKDGCGQFPYDANIQIHWVSFLDGRQRVLLFTDDVALVSKALQAEEMEQADYEITLSLHSLGLSLVNNESKQEVSYIGITSSGVVW-EVKPKQKWKPFSQKQIILLEQSY-------QKHQISRDHGWIKLDNNFEVNFDKDPMEMRLPIRSPIKRDFLSGIQIEFKQSSHQRSLRARLYWLQVDNQLPGAMFPVVFHPVAPPKSIALDSEPKPFIDVSVITRFNEYSKVLQFKYFMVLIQEMALKIDQGFLGAIIALFT---PTTDPEAERRRTKLIQQDIDALNAELMETSMTDMSILS-----FFEHFHISPVKLHLSLSLGSGGEESDKEKQEMFAVHS--VNLLLKSIGATLTDVDDLIFKLAYYEIRYQFYKRDQLIWSVVRHYSEQFLKQMYVLVLGLDVLGNPFGLIRGLSEGVEALFYEPFQGAVQGPEEFAEGLVIGVRSLFGHTVGGAAGVVSRITGSVGKGLAAITMDKEYQQKRREELSRQPRDFGDSLARGGKGFLRGVVGGVTGIITKPVEGAKKEGAAGFFKGIGKGLVGAVARPTGGIVDMASSTFQGIQRAAESTEEVSSLRPPRLIHEDGIIRPYDRQESEGSDL 3576          

HSP 3 Score: 317.775 bits (813), Expect = 1.467e-85
Identity = 317/1296 (24.46%), Postives = 578/1296 (44.60%), Query Frame = 3
Query: 3441 ITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKI--------LPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELI--RNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXX--XXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDA------YSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKP-CNSDEEFKVYLKADG---EVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDN---EGPSRGNVSEREIN-----TLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKC---YNVPIPT-----LLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKS---NTGVMYYYSVALSIEEVQIVNSSETLR-TYKLDINVPLALHNQLPIKISY--QSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAV 7184
            ITA L    V V ++K  NI DI IHGM A +      T+V   + D+ + +    +++KK + I  DE +F  Q+ L+   T    Y  D+S+VD  ++ +VG I+I++++ F + +  FLN+FQ + + ++    + +E AA+ + ++ ++  S R  +DIN+KAPVI IP++S S NA++ DLG +  +N F++      S   V+++  I L+ L++SR+IL  +  +N+  IL P+N+ + + RNL+      IP + I  ++  + V +S+ D  +   +L  N  EA +Q       +E V+ RK+        L + +    ++ + ++ +++QF F  E L + L+  D N     +       L +++   ++  G M  + +M VSV+L    + D +    +  + +I  +N ++++S  I I + QDKN  S++   +  +Y+C  V++LM + DFF+KA P        +  ++P R       K  K     P      +  +   +  PE++ V  +   D+ A+       L  S  K   ++   + D + + CPF  E R KN   ++ P S+ +      +     ++ ++  II I+P ++ ++  IM  L+ ++ +     DGS   +++   ++N W               G+      NT   GV++  ++ +    +E                  E    + +  I+V LE  +  + VP+L+ E    G++KN  + +     V++ V Y+N+   VWEPL+E    +       +++     D  L   +DF     I    ++I + S + + +T+SKS L+V +NL K F +       YSL          AP+ VKN  G  +         +KP CN        L+  G   +  ++D VD  Q+L  E         G    +SR+  S +   +    ++   N     P R   + R  N     +++V   A  G K + LRS LQ+ N+      +Y            KN  LLE +   +    ++VP+ +      +   G +    K+  T IS     W    +       +L C S   +   +   +VAL  E   I    E     Y + +   L L N LP  + Y  +    +H++  G    V  + +  +    V   YQ K W G   +   + E   + F + +    + +DL VH+     +  ++++SPYW++NKT   L Y++ +D  V+H A   D +
Sbjct: 1558 ITAELNAFNVFVCDQK-CNIADIKIHGMDASISVKPKQTDVFARLKDIIVMNVDLQSIHKKAVSILGDE-VFRFQLTLYPDATEGEAYA-DMSKVDGKLSFKVGCIQIVYVHKFFMSLLNFLNNFQTAKEALSTATVQAAERAASSMKDLAQK--SFRLLMDINLKAPVIIIPQSSVSPNAVIADLGLIRVENKFSLVPMEHYSLPPVIDKMNIELTQLKLSRTILQASLPQNDIEILKPVNMLLSIQRNLAAAWYVQIPGMEIKGKLKPMQVALSEDDLTVLMKILLENLGEASSQPSPTQSVQETVRVRKVDVSSVPDHLKEQEDWTDSKLSMNQIVSLQFDFHFESLSIILYNNDINQESGVAFHNDSFQLGELRLHLMASSGKMFKDGSMNVSVKLKTCTLDDLREGIERATSRMIDRKNDQDNNSSMIDISYKQDKN-GSQIDAVLDKLYVCASVEFLMTVADFFIKAVPQSPENVAKETQILP-RQTATGKVKIEKDDSVRP------NMTLKAMITDPEVVFVASLTKADAPALTASFQCNLSLSTSKLEQMMEASVRDLKVLACPFLREKRGKNITTVLQPCSLFMEKCTWASGKQNINIMVKEFIIKISPIILNTVLTIMAALSPKTKE-----DGSKDTSKE---MENLW---------------GIKSINDYNTWFLGVDTATEITESFKGIEHSLI--------------EENCGVVVESIQVTLECGLGHRTVPLLLAESKFSGNIKNWTSLMAAVADVTLQVHYYNEIHAVWEPLIERVEGKRQWNLRLDVKKNPVQDKSLLPGDDF-----IPEPQMAIHISSGNTMNITISKSCLNVFNNLAKGFSEGTASTFDYSLKDR-------APFTVKNAVGVPI--------KVKPNCN--------LRVMGFPEKSDIFD-VDAGQNLELEYASMVPSSQGNLSILSRQESSFFTLTIVPHGYTEVANIPVARPGRRLYNVRNPNASHSDSVLVQIDATEGNKVITLRSPLQIKNHFSIAFIIYKF---------VKNVKLLERIGIARPEEEFHVPLDSYRCQLFIQPAGILEHQYKESTTYIS-----WKEELHRSREVRCMLQCPSVEVSFLPLIVNTVALPDELSYICTHGEDWDVAYIIHLYPSLTLRNLLPYSLRYLLEGTAETHELAEGSTADVLHSRISGEIMELVLVKYQGKNWNGHFRIRDTLPEFFPVCF-SSDSTEVTTVDLSVHVRRIGSRMVLSVFSPYWLINKTTRVLQYRS-EDIHVKHPADFRDII 2758          

HSP 4 Score: 57.3806 bits (137), Expect = 7.959e-7
Identity = 36/120 (30.00%), Postives = 66/120 (55.00%), Query Frame = 3
Query: 2793 EKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIP 3152
            EK G + + +L V ++G    +K +DM  +AY+  + +    F D S   P+ +  +   T  + LL + L   +   P+F T+H +T+ +L+  F S+++ LH EALL  ++F++S  P
Sbjct: 1400 EKKG-RPLHELNVLQLGMEAKVKTYDMTAKAYLKKISMQCFDFTD-SKGEPLHIINSSNVTD-EPLLKMLLTKADSDGPEFKTIHDSTKQRLKVSFASLDLVLHLEALLSFMDFLSSAAP 1516          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Celegans
Match: T08G11.1 (pep chromosome:WBcel235:I:8905644:8916805:-1 gene:WBGene00011629.1 transcript:T08G11.1a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T08G11.1)

HSP 1 Score: 676.396 bits (1744), Expect = 0.000e+0
Identity = 671/2479 (27.07%), Postives = 1181/2479 (47.64%), Query Frame = 3
Query: 2835 EIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLIT-DRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAAN----KITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAID-QSTSVVLEESKITLSNLRVSRSILLEN--TVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKIL-----PKDDKSGSARTASDESITVQ-------FGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFF--VXXXXXXXXXXXXXMVPVRGYEKNPRKYRK-----KPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDI---INVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGS-LTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQL-IVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGE-VQLY-----DAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQL-ELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNT------GVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQSLDT-----VESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKI-NDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMM-MRCKVE-DREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMK---AYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCE--LSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVP--TYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA-------LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFS 10070
            ++   L ++ FDM V A +GS+ +++P+F     K            +G  L+ +  +     SP F T +A T   ++ +F  + V LHQE +L +  F  +L   LN                E +K T  EK+ E       +K   S+M + +  R     EKR  +    + +++ D +                +  I AS   L +++  +K      ++I  + A+VK +    EV  T+  + ++D +P AVYKK++ +   E +       + Q T      +  S VD +V + + +++ +FL  ++ R+  +L  FQ      A  AQ  +   A      +  ++E+ P  R +LD+ ++AP I +P  S S + +++ LG L  KN+   D +    +++   I +++      ++ E+   V +   IL PI+  + + RNL++     +P+I + A + SI   MS  DY      L  N  E  +     P     ++S         P  +K    R A+     ++       F F ++ +   L++G+A       E         ++ +N+   G +  ++++  ++ L     +D +     KI++L+    +    F+ + F QD  +N ++ + ++  ++C+C ++L  LT FF   +++    +    A VP +    +P          K  + P +P +    ID  +   E++LVED ++P+S   ++ +  V   S   E      +NV + +       ++S  R + T  +++P  I    N         + L M  L I ++P++I+ +S +    +  S    +   GS      +L    N+++ +  D  ++ F +  + +        + VE   DV Q     ++ R D            KES   + +  I   LE+      VP++  +  ++ +  +  + + +   VSV +SY+N+ ++VWEP++E    E      +++  ++       D  D      ++  A  +L ++     L++     +V +   K      + HL        +P+VV N  G  V     K  D +     E  +      GE V ++       V++++  I +E+    +KF  A ++ RE   I R E R +   P+ ++G  +          LIV+ T     ++L  L S ++  N+    + +Y       K +T  +  L   V  G+   + +P L + +G IY+       E+S     W+   N + +  Q + C++++      G+  Y    +  E++Q     +T   Y + ++ PL  HN LP  I+ +       +  G   L+        S +K    Y  +++   + +    +++ V+    + G  E  + LG+H   + G   V +Y+P+W++N T   L +       + + A                        +++V   ++  ++Q L T     +  PA DL                      +K  KA ++I N S WS++F LDTVG++ ++ CK     +++ V I L  SGLTKIVTF P+Y++ N     +   E  +  GW+ +P    V  WP     + +M +R K E + E L+   FP  E++  L  ++ + +G+ V V   E +  I+L  +  GM  V+++N+L   V + F Q    +  + P  +   ++W    + +  +  V+       + L  +   D  I++     +Y+ +F+    R+LLFT D+ +AK A  S + + +D +  ISL   GLS+VDN + +E+ Y++I+SS + W +  +K R KPL +   + LE   +++   L T   + E     +E  EV++++ +M K +  E  L R+FE G++  Y  S  +  +H +I  IQIDNQ+  C FP V +  PPP+SV +D   KP IELS +  +   +     +Y  VLIQE  + +DQG +N++L  +SG V    Y +E MF +D ++   +L ET++  T  S   K  +++ HISPI +HLSFS  +   +G  A       + SE++N+ L+SVG+ +T++QDV+FKL YFE++       Q+ S I SHY  Q ++Q+YVLVLGLD++GNPFG++R ++ GVEDLFY+P +GA+ GPEEF  GVA GV+S+FGHAVGGAAGAV RI GT+GKG+AALT D DY +KR+E L ++P++ G G+A+G KGL +G V G+TGVVTKPI+GAK++G  GF KGVGKGL+GVVTRPVSGVVDFAS +   +R VA TN E   +RPPR + +D I++ Y   +A G  ++ K T++ +    + +V +    S+K V+ +T++R+    R +++G+W  +W   Y K+  P  I    + +   LKEK++    
Sbjct:  872 KMACKLQMRTFDMVVTAELGSIKISMPEFKSLDEKREHLYLIDNDELQGA-LMTLKFVQANPESPFFATEYALTEQSVDFRFTKLAVSLHQEGVLELKAFGEALQAQLN----------------ELQKNTPEEKKVEETARKISRKLSDSVMSIASMSRTPSGKEKRQRKKTVGSATQEMDVS-------------RNIKQRIKASFGSLALMIGTQKHLET-SLAIENINANVKITEKAMEVVATLRAISMKDRTPGAVYKKLLSVTGKEDMLRFDFVQY-QRTDEQRALMKSSDVDMVVKMRLAQMRFVFLNLWLARLMAWLAPFQNEAVLAAQAAQAAAAEKAATAAQNVKQIMEQSPP-RIQLDVILEAPFIVVPRLSTSRDVIVLHLGRLALKNEIRGDSEYPKAIIDRMDILMTDCSFGMGVMNEDVSAVSSSCLILKPISFKLALQRNLTFAVAKELPEIVVDAHIHSIEAEMSDADYKTLMQTLSGNLAEGADLAIPPPPPPSSLESSNTAAALTSPGKEKEKKDREANAGPPVIEKSHTRIVFQFVLDKISAVLYEGEAV----NGERKESDAFAALRLKNVKTSGKIGEDNSIVFAMSLDAFT-MDDERKEKTKISKLMDKKGSKDDRFLDMSFNQDAEANKQIRLKMSAFFICLCPEFLGCLTRFFNVPQSEEQLEKQAVTANVPTKAVSASPSASSTFTGGTKTSSGPPQP-VGTLAIDCDMHGVEVILVEDSMHPESTQALILSFNVTAASHPNEQTKDTKMNVAVENLTIFSSYYQSSRRNEVTYQVLTPVRIEALVNMNTERKTTDAVLKMSALDIKMSPSIIRLLSAVSAEFSKSSAPGET---GSISQKAAKLRKWPNYFDSKPIDHRKYWFFAAPVAQ--------EAVEEELDVTQ----EDKSRTDSMIG--------KESA-KVDIERISFTLEAGTGAIPVPLIFLDMLVNAEAYDWSSAMRVSSGVSVQMSYYNESVSVWEPIIEPVENEKGEFERWKLA-MTMKSRNKQDSSDSSPQTEVKIEADKMLNVTVTKSLLSLLNKLSEVFATAAKQITPTKTRHLPGI-----SPFVVLNETGISV-----KVLDTETIRVSENGQAVDATHGEFVDVFLKNRKSDVEDRRLSIEQEEITGDLKFELAGTV-RE-TKIGRAEKRVIHL-PRVSDGGHKW---------LIVAETTVENSRRLVTLNSHVKFTNHLSYAVEIY------SKRDTTLD--LFGTVEHGETIPLAVPLLFSPSGDIYLKPVDDKYEVSFESLCWH---NFEHNRRQAVRCEADSTDGSFSGI--YIDSVVHEEKIQDGIDDQTTSIYHVHLHPPLEFHNNLPFDINIELP-EQKVLGAGMSTLLNVVA---GSPVKAWLTYLGEKYVLDMSIPEIKKDVEVVALNTETGSDE--LLLGLHWTSEYGDQKVYLYAPFWLVNNTDKMLRHVESSGNMLSNTARGCVPCG----------------KKSVVTSQENDDAVQHLPTENPIILPFPAIDL----------------------SKKKKARVRIENLSEWSEEFPLDTVGNAARITCKGSEHDFDLTVDIKLCQSGLTKIVTFAPFYLVSNLGKNPMEIREEGQK-GWVDIPAETCVGIWPVERKKRKLMCVRYKEEPEAESLL---FPITENYETLCHMDGEAIGVEVSVSTGESSVAIHLSSFVPGMCPVQVMNNL--TVPVTFGQKGHIKTTVGPN-EFAHFSWSSIIEPK--VLEVDIGDWHFEDKLDQNRFGDLQIDKSVRRFSYYSNFLIGRQRVLLFTPDVDIAKAAYGSWETDVVDMQAEISLQGFGLSVVDNIVGREIIYMAISSSDILWEEEVKKGRFKPLAVKYMQVLE---EKYQAHLVTPNDDYE----AMEAFEVNVNRMIMKKKKGKEVKLRRIFEKGLWACYGKSSQRTRLHAKINHIQIDNQLDACIFPRVLSVVPPPKSVIVDNTPKPFIELSLLQRQPEFSSIAEIEYGHVLIQEFSVQVDQGLINALLLLISGEVTRKPYGKE-MFDEDIKICHVTLSETAS--TYRSQRPKSFYNDLHISPIMMHLSFS--QGGTSGDVAASGVSMPIQSEMINVLLRSVGVTLTELQDVVFKLAYFERKCVFYSPEQLNSEIISHYAKQFIKQVYVLVLGLDIIGNPFGLVRDLSAGVEDLFYQPFQGAIQGPEEFAAGVALGVQSMFGHAVGGAAGAVGRITGTVGKGVAALTFDDDYMKKRQEDLNRKPQSFGEGMARGLKGLGMGVVGGITGVVTKPIEGAKQEGGFGFVKGVGKGLIGVVTRPVSGVVDFASGTMNSVRAVAGTNREAGPLRPPRVLREDKIVKPYSSGDAYGFKVF-KDTDRGELAETDEFVTYASI-SEKMVLIITDRRLVLSKRTDMMGVWQTEWGSEYCKIKEPEFI---PNGVKILLKEKKKGFLG 3181          

HSP 2 Score: 465.692 bits (1197), Expect = 4.805e-131
Identity = 282/819 (34.43%), Postives = 460/819 (56.17%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSS-ENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPET--TNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNV-PMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKK--MRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGT-----KGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQT 2447
            MVFESLV +L+N++LGD+V  LD SQL +G+ GG+  L+NL +KE A DDFDLP+K++ G++S L LKIPWK+LY+EPVIA +DGL ++++PN  + Y+ EK    + E KQK L R+EE + +R  PK      D+F EK+  QI+KNLQIS+SNIH+R+ED  T  +R FA+GITL  L+F+T D +    I K D  +  +K V + +LA+YWNS+ +  S  +++  ++  ++  I + +      +++++PI   A + ++ KPET  +N+ +P++ L + +  +  A  + QY D+  FL++ +R     ++ KYRPN+   + +   WW F Y  ILEE+VRRRR  WSW  +Q HR   R Y   W ++  +       Q TIK  E++LD+ N+ + RQQAE++  +  + ++ ++ T    W   WFG     T       G+ ++  + +   T EEKAKL++AI Y E     + P E+V  K + +L ++  ++ D ++   +L+KLV     A V+QR SA A+ V   +  +++ G      +  D S+P           M+ L+   +TNPL  + +Q + +   P+ I Y +  +N+ +    PPE ++L +++   ++  EEVK  +ATGL +  + R    + V I+P+   + E G Y+ +   LL D+G + +V+   S   S DT       G      + E AYD+FH+ LS +     E  +       ++ S  H+L+P G +  + K  I +D  L K+ V G LP++ + ISD  L  +++L L IP P+ 
Sbjct:    1 MVFESLVADLLNRFLGDFVDNLDSSQLNIGIWGGDVKLDNLQVKETALDDFDLPIKLKYGYLSSLVLKIPWKNLYNEPVIATVDGLNLIVVPNKGVVYNEEKVAKNIQEIKQKTLARLEEARKERRKPKD--PQADTFTEKMITQIIKNLQISVSNIHVRFEDRYTNRHRPFAMGITLEKLNFKTTDENWRETIHK-DVVKIIYKLVSLQNLAIYWNSSTEFISDLDDKEAIRKKLQETIHNGKNGPEGYNYILEPIQMEAKLKLNQKPETDGSNWKIPKIDLAVDMHALSIAIGKFQYQDVLLFLEAQERFNAAGQYLKYRPNLNEFKGHYKAWWKFAYTSILEEKVRRRRNNWSWDRMQKHRQLVRKYQKAWVRRQTEASPGADVQATIKEAEKKLDVFNVNVARQQAELEIDREGLTRQEDKPTGWVAWGKSWFGGGGGPTPDK--KKGAKDIGSQFQEAMTPEEKAKLFEAIDYQENIPPTNYPKEFVENKFDFKLGQVAIVV-DGAVS-MQLLKLV-----ASVEQRPSASAMHVESSIQELRMDGCGTEIIRVRDPSIP----------WMSFLL---DTNPLKGNYDQLVKLAVAPINIKYQAPAINNAIDVFKPPESVRLNQLTALAMSRYEEVKARSATGLAYAVEHRSRLVLDVQIQPARIYVSEGGTYSSEKPTLLADMGLLSVVTVDNS---SVDT------SGMNKMAALMEKAYDRFHVKLSNVVIAFAENVETAENCVFEKESPLHVLKPTGLDIQIHKSSI-DDLKLAKMRVIGDLPNIVIGISDDRLIGLMKLGLSIPTPKA 784          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Celegans
Match: T08G11.1 (pep chromosome:WBcel235:I:8905644:8916805:-1 gene:WBGene00011629.1 transcript:T08G11.1b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T08G11.1)

HSP 1 Score: 484.952 bits (1247), Expect = 6.518e-137
Identity = 352/914 (38.51%), Postives = 546/914 (59.74%), Query Frame = 3
Query: 7380 FAKTVKAHLKI-NDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMM-MRCKVE-DREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMK---AYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCE--LSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVP--TYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA-------LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFS 10070
             +K  KA ++I N S WS++F LDTVG++ ++ CK     +++ V I L  SGLTKIVTF P+Y++ N     +   E  +  GW+ +P    V  WP     + +M +R K E + E L+   FP  E++  L  ++ + +G+ V V   E +  I+L  +  GM  V+++N+L   V + F Q    +  + P  +   ++W    + +  +  V+       + L  +   D  I++     +Y+ +F+    R+LLFT D+ +AK A  S + + +D +  ISL   GLS+VDN + +E+ Y++I+SS + W +  +K R KPL +   + LE   +++   L T   + E     +E  EV++++ +M K +  E  L R+FE G++  Y  S  +  +H +I  IQIDNQ+  C FP V +  PPP+SV +D   KP IELS +  +   +     +Y  VLIQE  + +DQG +N++L  +SG V    Y +E MF +D ++   +L ET++  T  S   K  +++ HISPI +HLSFS  +   +G  A       + SE++N+ L+SVG+ +T++QDV+FKL YFE++       Q+ S I SHY  Q ++Q+YVLVLGLD++GNPFG++R ++ GVEDLFY+P +GA+ GPEEF  GVA GV+S+FGHAVGGAAGAV RI GT+GKG+AALT D DY +KR+E L ++P++ G G+A+G KGL +G V G+TGVVTKPI+GAK++G  GF KGVGKGL+GVVTRPVSGVVDFAS +   +R VA TN E   +RPPR + +D I++ Y   +A G  ++ K T++ +    + +V +    S+K V+ +T++R+    R +++G+W  +W   Y K+  P  I    + +   LKEK++    
Sbjct: 2267 LSKKKKARVRIENLSEWSEEFPLDTVGNAARITCKGSEHDFDLTVDIKLCQSGLTKIVTFAPFYLVSNLGKNPMEIREEGQK-GWVDIPAETCVGIWPVERKKRKLMCVRYKEEPEAESLL---FPITENYETLCHMDGEAIGVEVSVSTGESSVAIHLSSFVPGMCPVQVMNNL--TVPVTFGQKGHIKTTVGPN-EFAHFSWSSIIEPK--VLEVDIGDWHFEDKLDQNRFGDLQIDKSVRRFSYYSNFLIGRQRVLLFTPDVDIAKAAYGSWETDVVDMQAEISLQGFGLSVVDNIVGREIIYMAISSSDILWEEEVKKGRFKPLAVKYMQVLE---EKYQAHLVTPNDDYE----AMEAFEVNVNRMIMKKKKGKEVKLRRIFEKGLWACYGKSSQRTRLHAKINHIQIDNQLDACIFPRVLSVVPPPKSVIVDNTPKPFIELSLLQRQPEFSSIAEIEYGHVLIQEFSVQVDQGLINALLLLISGEVTRKPYGKE-MFDEDIKICHVTLSETAS--TYRSQRPKSFYNDLHISPIMMHLSFS--QGGTSGDVAASGVSMPIQSEMINVLLRSVGVTLTELQDVVFKLAYFERKCVFYSPEQLNSEIISHYAKQFIKQVYVLVLGLDIIGNPFGLVRDLSAGVEDLFYQPFQGAIQGPEEFAAGVALGVQSMFGHAVGGAAGAVGRITGTVGKGVAALTFDDDYMKKRQEDLNRKPQSFGEGMARGLKGLGMGVVGGITGVVTKPIEGAKQEGGFGFVKGVGKGLIGVVTRPVSGVVDFASGTMNSVRAVAGTNREAGPLRPPRVLREDKIVKPYSSGDAYGFKVF-KDTDRGELAETDEFVTYASI-SEKMVLIITDRRLVLSKRTDMMGVWQTEWGSEYCKIKEPEFI---PNGVKILLKEKKKGFLG 3154          

HSP 2 Score: 466.463 bits (1199), Expect = 3.117e-131
Identity = 282/819 (34.43%), Postives = 460/819 (56.17%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSS-ENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPET--TNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNV-PMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKK--MRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGT-----KGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQT 2447
            MVFESLV +L+N++LGD+V  LD SQL +G+ GG+  L+NL +KE A DDFDLP+K++ G++S L LKIPWK+LY+EPVIA +DGL ++++PN  + Y+ EK    + E KQK L R+EE + +R  PK      D+F EK+  QI+KNLQIS+SNIH+R+ED  T  +R FA+GITL  L+F+T D +    I K D  +  +K V + +LA+YWNS+ +  S  +++  ++  ++  I + +      +++++PI   A + ++ KPET  +N+ +P++ L + +  +  A  + QY D+  FL++ +R     ++ KYRPN+   + +   WW F Y  ILEE+VRRRR  WSW  +Q HR   R Y   W ++  +       Q TIK  E++LD+ N+ + RQQAE++  +  + ++ ++ T    W   WFG     T       G+ ++  + +   T EEKAKL++AI Y E     + P E+V  K + +L ++  ++ D ++   +L+KLV     A V+QR SA A+ V   +  +++ G      +  D S+P           M+ L+   +TNPL  + +Q + +   P+ I Y +  +N+ +    PPE ++L +++   ++  EEVK  +ATGL +  + R    + V I+P+   + E G Y+ +   LL D+G + +V+   S   S DT       G      + E AYD+FH+ LS +     E  +       ++ S  H+L+P G +  + K  I +D  L K+ V G LP++ + ISD  L  +++L L IP P+ 
Sbjct:    1 MVFESLVADLLNRFLGDFVDNLDSSQLNIGIWGGDVKLDNLQVKETALDDFDLPIKLKYGYLSSLVLKIPWKNLYNEPVIATVDGLNLIVVPNKGVVYNEEKVAKNIQEIKQKTLARLEEARKERRKPKD--PQADTFTEKMITQIIKNLQISVSNIHVRFEDRYTNRHRPFAMGITLEKLNFKTTDENWRETIHK-DVVKIIYKLVSLQNLAIYWNSSTEFISDLDDKEAIRKKLQETIHNGKNGPEGYNYILEPIQMEAKLKLNQKPETDGSNWKIPKIDLAVDMHALSIAIGKFQYQDVLLFLEAQERFNAAGQYLKYRPNLNEFKGHYKAWWKFAYTSILEEKVRRRRNNWSWDRMQKHRQLVRKYQKAWVRRQTEASPGADVQATIKEAEKKLDVFNVNVARQQAELEIDREGLTRQEDKPTGWVAWGKSWFGGGGGPTPDK--KKGAKDIGSQFQEAMTPEEKAKLFEAIDYQENIPPTNYPKEFVENKFDFKLGQVAIVV-DGAVS-MQLLKLV-----ASVEQRPSASAMHVESSIQELRMDGCGTEIIRVRDPSIP----------WMSFLL---DTNPLKGNYDQLVKLAVAPINIKYQAPAINNAIDVFKPPESVRLNQLTALAMSRYEEVKARSATGLAYAVEHRSRLVLDVQIQPARIYVSEGGTYSSEKPTLLADMGLLSVVTVDNS---SVDT------SGMNKMAALMEKAYDRFHVKLSNVVIAFAENVETAENCVFEKESPLHVLKPTGLDIQIHKSSI-DDLKLAKMRVIGDLPNIVIGISDDRLIGLMKLGLSIPTPKA 784          

HSP 3 Score: 220.32 bits (560), Expect = 1.564e-56
Identity = 312/1484 (21.02%), Postives = 618/1484 (41.64%), Query Frame = 3
Query: 2835 EIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLIT-DRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAAN----KITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAID-QSTSVVLEESKITLSNLRVSRSILLEN--TVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKIL-----PKDDKSGSARTASDESITVQ-------FGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFF--VXXXXXXXXXXXXXMVPVRGYEKNPRKYR-----KKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKES---DIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGS-LTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQL-IVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGE-VQLY-----DAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQL-ELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSN------TGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQ 7151
            ++   L ++ FDM V A +GS+ +++P+F     K            +G  L+ +  +     SP F T +A T   ++ +F  + V LHQE +L +  F  +L   LN                E +K T  EK+ E       +K   S+M + +  R     EKR  +    + +++ D +                +  I AS   L +++  +K      ++I  + A+VK +    EV  T+  + ++D +P AVYKK++ +   E +       + Q T      +  S VD +V + + +++ +FL  ++ R+  +L  FQ      A  AQ  +   A      +  ++E+ P  R +LD+ ++AP I +P  S S + +++ LG L  KN+   D +    +++   I +++      ++ E+   V +   IL PI+  + + RNL++     +P+I + A + SI   MS  DY      L  N  E  +     P     ++S         P  +K    R A+     ++       F F ++ +   L++G+A       E         ++ +N+   G +  ++++  ++ L     +D +     KI++L+    +    F+ + F QD  +N ++ + ++  ++C+C ++L  LT FF   +++    +    A VP +    +P          K  + P +P +    ID  +   E++LVED ++P+S   ++ +  V   S   E      +NV + +       ++S  R + T  +++P  I    N         + L M  L I ++P++I+ +S +    +  S    +   GS      +L    N+++ +  D  ++ F +  + +        + VE   DV Q     ++ R D            KES   + +  I   LE+      VP++  +  ++ +  +  + + +   VSV +SY+N+ ++VWEP++E    E      +++  ++       D  D      ++  A  +L ++     L++     +V +   K      + HL        +P+VV N  G  V     K  D +     E  +      GE V ++       V++++  I +E+    +KF  A ++ RE   I R E R +   P+ ++G  +          LIV+ T     ++L  L S ++  N+    + +Y       K +T  +  L   V  G+   + +P L + +G IY+       E+S     W+   N + +  Q + C+++      +G+  Y    +  E++Q     +T   Y + ++ PL  HN LP  I+ +       +  G   L+        S +K    Y  +++   + +    +++ V+    + G  E  + LG+H   + G   V +Y+P+W++N T   L +   D  Q
Sbjct:  872 KMACKLQMRTFDMVVTAELGSIKISMPEFKSLDEKREHLYLIDNDELQGA-LMTLKFVQANPESPFFATEYALTEQSVDFRFTKLAVSLHQEGVLELKAFGEALQAQLN----------------ELQKNTPEEKKVEETARKISRKLSDSVMSIASMSRTPSGKEKRQRKKTVGSATQEMDVS-------------RNIKQRIKASFGSLALMIGTQKHLET-SLAIENINANVKITEKAMEVVATLRAISMKDRTPGAVYKKLLSVTGKEDMLRFDFVQY-QRTDEQRALMKSSDVDMVVKMRLAQMRFVFLNLWLARLMAWLAPFQNEAVLAAQAAQAAAAEKAATAAQNVKQIMEQSPP-RIQLDVILEAPFIVVPRLSTSRDVIVLHLGRLALKNEIRGDSEYPKAIIDRMDILMTDCSFGMGVMNEDVSAVSSSCLILKPISFKLALQRNLTFAVAKELPEIVVDAHIHSIEAEMSDADYKTLMQTLSGNLAEGADLAIPPPPPPSSLESSNTAAALTSPGKEKEKKDREANAGPPVIEKSHTRIVFQFVLDKISAVLYEGEAV----NGERKESDAFAALRLKNVKTSGKIGEDNSIVFAMSLDAFT-MDDERKEKTKISKLMDKKGSKDDRFLDMSFNQDAEANKQIRLKMSAFFICLCPEFLGCLTRFFNVPQSEEQLEKQAVTANVPTKAVSASPSASSTFTGGTKTSSGPPQP-VGTLAIDCDMHGVEVILVEDSMHPESTQALILSFNVTAASHPNEQTKDTKMNVAVENLTIFSSYYQSSRRNEVTYQVLTPVRIEALVNMNTERKTTDAVLKMSALDIKMSPSIIRLLSAVSAEFSKSSAPGET---GSISQKAAKLRKWPNYFDSKPIDHRKYWFFAAPVAQ--------EAVEEELDVTQ----EDKSRTDSMIG--------KESA-KVDIERISFTLEAGTGAIPVPLIFLDMLVNAEAYDWSSAMRVSSGVSVQMSYYNESVSVWEPIIEPVENEKGEFERWKLA-MTMKSRNKQDSSDSSPQTEVKIEADKMLNVTVTKSLLSLLNKLSEVFATAAKQITPTKTRHLPGI-----SPFVVLNETGISV-----KVLDTETIRVSENGQAVDATHGEFVDVFLKNRKSDVEDRRLSIEQEEITGDLKFELAGTV-RE-TKIGRAEKRVIHL-PRVSDGGHKW---------LIVAETTVENSRRLVTLNSHVKFTNHLSYAVEIY------SKRDTTLD--LFGTVEHGETIPLAVPLLFSPSGDIYLKPVDDKYEVSFESLCWH---NFEHNRRQAVRCEADSTDGSFSGI--YIDSVVHEEKIQDGIDDQTTSIYHVHLHPPLEFHNNLPFDINIELP-EQKVLGAGMSTLLNVVA---GSPVKAWLTYLGEKYVLDMSIPEIKKDVEVVALNTETGSDE--LLLGLHWTSEYGDQKVYLYAPFWLVNNTDKMLRHVNDDAVQ 2250          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Celegans
Match: C25H3.11 (pep chromosome:WBcel235:II:5669788:5689030:-1 gene:WBGene00016120.1 transcript:C25H3.11b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C25H3.11)

HSP 1 Score: 169.474 bits (428), Expect = 4.013e-41
Identity = 114/391 (29.16%), Postives = 199/391 (50.90%), Query Frame = 3
Query:   27 VFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIP-NSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKE------TETKDS-FGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSS-ENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFT----VPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTK----NRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLL 1148
            + E++V  ++N Y+G+Y+  L+  QL + LL G   LEN+ +K+ A    DLPV+V+ G + KLTL +P  H+ SEP   +L  + +++ P +SE +YD E       E ++KE   +EE +L+      +       E++DS +G  L + ++ N+Q+ ++N+HIRYED +T P + F  GI +  ++ QT +        +       FK +++   ++YWNS      + E+   LK I+    SS+       +++IQP  CSA   + M+  ++ F     VPR    ++ + +G    R+Q       L +++R W +     + RK+RP   +  N   WW F YN +LEE  RR     +W +     +R   Y   + ++L+
Sbjct:    1 MLENVVAWVLNNYIGEYLEDLNTDQLSVALLSGQVELENVPLKKTALRKLDLPVEVKSGLLGKLTLSVPITHIRSEPWTLKLSDVLIIVGPLSSEKRYDVEAVEQ--VEQQRKE-QMLEELELRHKTALFDLCGIPIPESQDSWWGASLISTVLNNIQLILNNVHIRYEDDTTIPGQTFNCGIRIQKMTMQTTNHHWKPGFAEPQKGTNTFKKLELGGFSIYWNSKTTMTENIEDSKQLKLILAPETSSN-------AYIIQP--CSA--ELRMEKNSSKFPLKSKVPRFKFFMRPDILGLELTRHQMTQ----LRAVNREWARFERARQHRKWRPLCTIGENAKEWWKFAYNRVLEES-RRANANRTWDFAHERASRFNAYCRAYRKRLI 372          

HSP 2 Score: 60.8474 bits (146), Expect = 2.884e-8
Identity = 40/169 (23.67%), Postives = 79/169 (46.75%), Query Frame = 3
Query: 8976 HLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAK 9482
            H+  S+  V+++G        L L  Q   + +   ++    L  F + +     + ++ S+   Y+++  +Q   +++ LD  GNP G++  +    + LF E   G V   + FV G+ YG+ +           +VS++  +M  G+ ALT D D++ KRR  + +
Sbjct: 2900 HVGLSVVTVSKSGL----PRDLRLLKQQFNVKLISFENASVALPPFRQYHYFETSSFLLESLQKFYLAELQKQTLSIIVTLDAFGNPLGLVTDLKDSFQGLFIE---GDV---QRFVAGLGYGLSN-----------SVSKVASSMASGVGALTFDQDHELKRRHNMIR 3047          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Celegans
Match: C25H3.11 (pep chromosome:WBcel235:II:5669788:5689030:-1 gene:WBGene00016120.1 transcript:C25H3.11a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C25H3.11)

HSP 1 Score: 169.474 bits (428), Expect = 4.257e-41
Identity = 114/391 (29.16%), Postives = 199/391 (50.90%), Query Frame = 3
Query:   27 VFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIP-NSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKE------TETKDS-FGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSS-ENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFT----VPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTK----NRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLL 1148
            + E++V  ++N Y+G+Y+  L+  QL + LL G   LEN+ +K+ A    DLPV+V+ G + KLTL +P  H+ SEP   +L  + +++ P +SE +YD E       E ++KE   +EE +L+      +       E++DS +G  L + ++ N+Q+ ++N+HIRYED +T P + F  GI +  ++ QT +        +       FK +++   ++YWNS      + E+   LK I+    SS+       +++IQP  CSA   + M+  ++ F     VPR    ++ + +G    R+Q       L +++R W +     + RK+RP   +  N   WW F YN +LEE  RR     +W +     +R   Y   + ++L+
Sbjct:    1 MLENVVAWVLNNYIGEYLEDLNTDQLSVALLSGQVELENVPLKKTALRKLDLPVEVKSGLLGKLTLSVPITHIRSEPWTLKLSDVLIIVGPLSSEKRYDVEAVEQ--VEQQRKE-QMLEELELRHKTALFDLCGIPIPESQDSWWGASLISTVLNNIQLILNNVHIRYEDDTTIPGQTFNCGIRIQKMTMQTTNHHWKPGFAEPQKGTNTFKKLELGGFSIYWNSKTTMTENIEDSKQLKLILAPETSSN-------AYIIQP--CSA--ELRMEKNSSKFPLKSKVPRFKFFMRPDILGLELTRHQMTQ----LRAVNREWARFERARQHRKWRPLCTIGENAKEWWKFAYNRVLEES-RRANANRTWDFAHERASRFNAYCRAYRKRLI 372          

HSP 2 Score: 60.8474 bits (146), Expect = 2.860e-8
Identity = 40/169 (23.67%), Postives = 79/169 (46.75%), Query Frame = 3
Query: 8976 HLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAK 9482
            H+  S+  V+++G        L L  Q   + +   ++    L  F + +     + ++ S+   Y+++  +Q   +++ LD  GNP G++  +    + LF E   G V   + FV G+ YG+ +           +VS++  +M  G+ ALT D D++ KRR  + +
Sbjct: 2902 HVGLSVVTVSKSGL----PRDLRLLKQQFNVKLISFENASVALPPFRQYHYFETSSFLLESLQKFYLAELQKQTLSIIVTLDAFGNPLGLVTDLKDSFQGLFIE---GDV---QRFVAGLGYGLSN-----------SVSKVASSMASGVGALTFDQDHELKRRHNMIR 3049          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Celegans
Match: C25H3.11 (pep chromosome:WBcel235:II:5669788:5689030:-1 gene:WBGene00016120.1 transcript:C25H3.11c.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C25H3.11)

HSP 1 Score: 169.474 bits (428), Expect = 4.289e-41
Identity = 116/385 (30.13%), Postives = 197/385 (51.17%), Query Frame = 3
Query:   27 VFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIP-NSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKE------TETKDS-FGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSS-ENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFT----VPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTK----NRFRKYRPNVPMRSNISIWWHFPYNVILEEQVR-RRRRMWSWKYIQAHR--NRCRLY 1121
            + E++V  ++N Y+G+Y+  L+  QL + LL G   LEN+ +K+ A    DLPV+V+ G + KLTL +P  H+ SEP   +L  + +++ P +SE +YD E       E ++KE   +EE +L+      +       E++DS +G  L + ++ N+Q+ ++N+HIRYED +T P + F  GI +  ++ QT +        +       FK +++   ++YWNS      + E+   LK I+    SS+       +++IQP  CSA   + M+  ++ F     VPR    ++ + +G    R+Q       L +++R W +     + RK+RP   +  N   WW F YN +LEE  R    R W + + +A R    CR Y
Sbjct:    1 MLENVVAWVLNNYIGEYLEDLNTDQLSVALLSGQVELENVPLKKTALRKLDLPVEVKSGLLGKLTLSVPITHIRSEPWTLKLSDVLIIVGPLSSEKRYDVEAVEQ--VEQQRKE-QMLEELELRHKTALFDLCGIPIPESQDSWWGASLISTVLNNIQLILNNVHIRYEDDTTIPGQTFNCGIRIQKMTMQTTNHHWKPGFAEPQKGTNTFKKLELGGFSIYWNSKTTMTENIEDSKQLKLILAPETSSN-------AYIIQP--CSA--ELRMEKNSSKFPLKSKVPRFKFFMRPDILGLELTRHQMTQ----LRAVNREWARFERARQHRKWRPLCTIGENAKEWWKFAYNRVLEESRRANANRTWDFAHERASRFNAYCRAY 367          

HSP 2 Score: 60.8474 bits (146), Expect = 2.907e-8
Identity = 40/169 (23.67%), Postives = 79/169 (46.75%), Query Frame = 3
Query: 8976 HLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAK 9482
            H+  S+  V+++G        L L  Q   + +   ++    L  F + +     + ++ S+   Y+++  +Q   +++ LD  GNP G++  +    + LF E   G V   + FV G+ YG+ +           +VS++  +M  G+ ALT D D++ KRR  + +
Sbjct: 2892 HVGLSVVTVSKSGL----PRDLRLLKQQFNVKLISFENASVALPPFRQYHYFETSSFLLESLQKFYLAELQKQTLSIIVTLDAFGNPLGLVTDLKDSFQGLFIE---GDV---QRFVAGLGYGLSN-----------SVSKVASSMASGVGALTFDQDHELKRRHNMIR 3039          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Fly
Match: Vps13 (gene:FBgn0033194 transcript:FBtr0310024)

HSP 1 Score: 523.857 bits (1348), Expect = 1.283e-148
Identity = 353/907 (38.92%), Postives = 534/907 (58.88%), Query Frame = 3
Query: 7368 KNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG-WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLE-KKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEM-KAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIR-RKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIR---ASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLF 10067
            +++ F    KA ++I++  WS+K  LD  GS G+V+C      Y VGV  +L+ + LTK +TF+P+Y++ N+    +   E    A  W+ + P E  P WPR++   ++++R   +     +T  F F E    L+KLE  K+ GI V+VQ TE    I    Y+   A   LINH G  +  H K   T  E IL A  T+MYAW D    +  ++   G  K+ ++ L  DGI +  + +  K  WVSF+    R+LLFT +  +A     +A L+SI Q I + +H IGLS+++N    ++ Y+ +TSSG+ W   +  KNR K L + ++  LE  + +++  +  S  +V+ + +      +D    ++ K  +  L R F P ++L  ++SP Q  +H++I +IQ+DNQ  D  FPVV AP PPP+SVA  T LKP IE S V      +   ++KY R+LIQE    +D  F+ +I +  +  V        F +D + I    ++  E  +++      +K  +DN H+ P+KIH+SFS++       T      L   +Q VG+ +TD+ DV+F+L +FE++       Q+++ ITSHY  QAL+Q+YVLVLGLDVLGNP+G++ G+ +GVEDLFYEP +GA+ GP EF EG+  GVKSLFGH VGGAAGAVS+I G MGKG+AALT D DYQ+KRR+ +  +P+N   GLA+  KGL++GFV GVTGVVTKP+ GA+ +G+EGFFKG+GKG +G+V RP +GVVDFAS SFE ++R AD + +V R+RPPRF   D ++R Y L EA GN I +K T+K      + ++       K   + +TN R+ ++ RNE+ G+W+  W   + ++    ++  +   + FT+K   +K+ 
Sbjct: 2394 RDKLFFDKKKASIRIDNGQWSEKIPLDVAGSVGEVICFANNQKYPVGVHNHLTQNSLTKQITFIPFYIVCNKCHFDIELQEQSRPADPWLHLEPNEMEPLWPRNDTKNNLVVRVDGK-----ITPAFDFTEVICTLLKLEDSKYGGINVDVQTTEGGVYITFTDYKPADAPGLLINHTGKQIVYHEK--GTKNEHILNAKSTIMYAWDDPTGPKMLVF---GTNKEETD-LKRDGIGEVIMQDGGKVLWVSFLDGLQRVLLFTENESIANRTESTASLQSITQSIDLRIHGIGLSVINNETGLDILYLGVTSSGIIWESKKVTKNRFKELTINENALLEIEYQKYL--VHKSVNDVQTYKLD-NKFPIDFDLMILKKTVERNLRRSFYPAIWLSRKSSPFQSQLHVKINRIQVDNQFLDPIFPVVLAPIPPPKSVASTTSLKPFIECSMVQRIMPNSTVRQFKYARILIQEFLFKVDLNFLTAIAEMFAKEVSDEAAAKQFRQDVESIELPLSAFFEEHSLEE-----QKSFYDNLHLGPLKIHVSFSMA----GSDTKALPGFLGSLVQGVGVTLTDVNDVVFRLAFFEREYQFFSQKQLINEITSHYTGQALKQLYVLVLGLDVLGNPYGLVVGLKKGVEDLFYEPFQGAIQGPGEFAEGLVLGVKSLFGHTVGGAAGAVSKITGAMGKGLAALTFDEDYQKKRRQGIQNKPKNFHEGLARSSKGLVMGFVDGVTGVVTKPVTGARDNGVEGFFKGLGKGAIGLVARPTAGVVDFASGSFEAVKRAADASEDVKRMRPPRFQHYDFVLRPYCLMEATGNKI-MKETDKGKFATTDNFIHCEEIIQKSEYLVVTNYRVMYVQRNEMFGVWTSLWSYLWNEIS---SVAATARGVQFTVKTDGKKVL 3273          

HSP 2 Score: 471.085 bits (1211), Expect = 1.455e-132
Identity = 276/829 (33.29%), Postives = 468/829 (56.45%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIE---ENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTN--FTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRP-NVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFII------SDNSLKEY-KLMKLVIHSMS---AHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKY-TKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRK-LRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPP 2456
            MVFE++V +++NK LGDY+  LD++QLK+G+ GG+  L+NL I+ENA D+ DLPV++  G++ KL LKIPWK+LYS+PVI  ++ LYVL+ PN+ ++Y+ EKE  Y  + K+  L+ +E   + +L+ + PK +      F EKL AQIV NLQ+ I+N+H+RYEDT+T     F+ GI+L  L   T D       + + ++ + FK   +  L+ Y N   + +++ N++ L    K NI+  E K  + ++++ PISC+A + ++M PE  +  F  P++ L L++E++       Q+ ++    D+M+R      +RKYRP N+P + +   WWHF    ILEE+VR+ R  W+W +I+ HR RC  Y   ++++ L KK S    +T ++ E ELD+ N+ L RQ+  ++  K R+ + ++ S      GW G +  +  ++     S +L +K  +  T EEK K+Y+AIGY E     D+P  Y ++++N +L  L+  +      S  + K++ +L  LV+ + S   A + QR +A+AIS+   M  ++VTG    D + P LV ++   +  NLL V FETNPLDK  +Q++ + A PL+I YD+ T+  ++     P ++ L +        I   KE +ATG++++ D++   D+ + + P+  ++P +G Y   +  +L+V +G + + S+              G+D       + E AYD+F + +  +Q L++  G+ W+  L    S++ H+LRP   +     CV+ ND  LP I V   LP + V +S+  +   +++   IP P+   P
Sbjct:    1 MVFEAVVADVLNKVLGDYIENLDRNQLKIGIWGGDVVLQNLKIRENALDELDLPVQLIYGYLGKLVLKIPWKNLYSQPVIVNIEDLYVLVSPNNNVQYNAEKEAKYEMDLKKAALDALEAARKKELEMDQPKADA----GFAEKLTAQIVNNLQVQITNVHLRYEDTTTT-GSPFSFGISLHELELYTTDCDWEKCYMAQQAS-QVFKIANLSCLSAYLNCGGQLYAN-NKSDLSQQFKTNIACKETK-PNYNYVLGPISCNAKLKLNMNPELDDPPFEKPKIDLTLEMEKLNVGLTNTQFDNLMKLGDAMNRQQLGIPYRKYRPYNIPYKGHARDWWHFAITSILEEEVRKPRESWTWGHIKTHRERCNTYAQKYKEQCLSKKPSAVLTETCRLLETELDVFNLLLIRQRVNIEIAKQREAVPEQKSGWFSGWGWGGGAKKDDQTT-----SQKLVEKFEAAMTSEEKEKMYRAIGYQENAKPTDLPESYEAIRMNFKLIALEVGLYKDERNSSAATKDFHELPSLVLLNFSMATALITQRPAAEAISIIAGMREIKVTGLTRNDYT-PLLVESKITDEF-NLLEVFFETNPLDKLCDQRVKVVARPLQITYDAPTILALINAFQTPGDVTLSKFEDAASTKISNFKERSATGMQYMIDKKAVLDVDILLMPNILVVPHKGVYDAGNVSLLVVSMGQVHLSSQPRRESNKLQHLFSAGEDKDEILKTVMENAYDRFTVAVDDVQMLVVRAGEPWQNALAEANSTEMHVLRPVSLKVTAALCVVDNDPRLPNIKVDIDLPAILVNVSEDRIFLAIKVATSIPLPEQKEP 814          

HSP 3 Score: 223.402 bits (568), Expect = 2.503e-57
Identity = 264/1102 (23.96%), Postives = 502/1102 (45.55%), Query Frame = 3
Query: 2961 TKKHTKGQNLLF-INLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMT-QWAITASLQKLRVIVANEKDNNILDISIHGMRAD--VKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKND----FAIDQSTSVVLEESKITLSNLRVSRSILLENT------VKNEKGIL------HPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESI---------TVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKI----------------NELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXM-VPVRGYEKNPRKYRKKP--LAS----PVEPRLAD-----FEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLL---EVYPEEHNRQYS-FEIEYISCDDSGLFD-EEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINK 6080
            T K     N LF ++  I +K+SP+F T + +T   +   F+ +++ LHQE L  ++  +N+    L++V               TR +  +                 SI G   D I     KRT+ +  E   E    +     KK     +  T +  + A+L ++ +++   K   I ++++    +   +K+SY  TEV+I + D+++ D +P  ++K I+ I   ++ F+ Q+ ++N+   T  Y  D    D  + +++G +KIIFL  FV  +  FLN+F  +  T++      +E+A  K  +  E   ++R KL+I +KAP+I +P  S+  NAL++DLG L   N+       ++    V++E K+ + ++++S+ +LL+        V  E G L      +P++  + +TRNLS+     +P+++++ R+ SI + +   DY +  LVL  N NE + + P   E+ +E + R   P+   S + R +    +         +++F F+ + + + L +G+               L +     LSV+G+   N  +  SV L NI + D + +S  +I                +E+I    N+ +  + +  I  K  ++   V + G  + +C+D+L+ LT F         +        PV    ++ +   +    LA+    PVE    +       + + +  P+I+LVE++ + +++ I+      L Y  + +  I+N  I   +   C F  E R+     I+ P  ISL  + P   GM  SL +  +IIN++PA I+ ++K M ++++ +  + +  + S   N       N W +  F    + F                 VE G D     L+ EQR       + DN ++ K  K  + +P I +++ES V     P++  +  +     N    +    +++++++Y+N  +  WEP++   EV      R+Y+ +E+++    + G+   + + ED    E  A+ + + S + LE+T+SK+ L +LS L +AF  A   +      I  APYV++N  G DV   + K
Sbjct:  973 TPKQENSSNYLFTVSCTIADKSSPEFSTKYNSTEQLVVANFEVLQIVLHQECLQRIMEVVNNFQRNLDLV------------LSSTRPRDRM----------------GSIGG--GDGI-----KRTLNVILEDTEEIMTTDQMKRRKKTRRTHVVETVKVRVIANLDQVGLVLTGRK-RPIAEMNVKKFVSSLIIKSSY--TEVNIGLKDIQVLDLNPYTIHKNILSIVGKDA-FNCQIVIYNK-EETQDYNSD----DMKITVDIGCMKIIFLNWFVAGVMNFLNNFTAAQATISQAGAAAAESARQKAMDAYET--ATRMKLNIRIKAPIIIVPIGSQDRNALLLDLGLLELTNNTVEVAVAEEERLAVIDEIKLQICDVKISKIVLLDGNESTVDEVDAEVGFLSKFNMMNPMSCTLSITRNLSYTWYRDVPELNLSGRLKSIELTLFADDYALVMLVLNRNLNEGLEEFPPSEEAPQEAQVR---PERRNSRAGRLSRTVQVSPIREKIHESIKFNFQFDGVVINLMEGEG------------AGLARFGIYFLSVKGTKLDNGTLSTSVVLCNIQMDDMRSNSKSQIRQYLSRKDWVQPKLDTDEIIDACYNERNFMVDVTAII-KEDDTFAEVRVRGFDLIVCIDFLLKLTTFLTLPPEENPRESVYIKPAPVSETARDTKHSIRSSAILAAQELVPVESSSHEVPNRKMNLILHIDEPDIILVENLEDLNTSCIIFNAQVHLNYRSINDKQIVNGQIDALKMYMCAFLPERREMTRHYILHPCVISLQGSTPEEEGMHISLKLSDIIINVSPATIELLNKAMLSVSSGTMTKCAIAEES--RN-----YSNLWHQHHFHSRTYWFTK---------------VEQGVDA----LEAEQRSV-----STDN-EKQKTEKCVIEIPSITLVIESGVGYYTKPLISLDTRITAVFNNWSRSLTAHGSLTLNMNYYNQALAEWEPIIELNEVIGRNGVREYTPWELKF----EMGMEKVQSELED--DAEQQAMHMNIHSAETLEITLSKTCLGLLSELAEAFSQAIDQNGLTKPDIV-APYVLENDTGFDVNLNLRK 1973          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Fly
Match: Vps13 (gene:FBgn0033194 transcript:FBtr0088910)

HSP 1 Score: 523.857 bits (1348), Expect = 1.283e-148
Identity = 353/907 (38.92%), Postives = 534/907 (58.88%), Query Frame = 3
Query: 7368 KNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG-WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLE-KKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEM-KAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIR-RKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIR---ASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLF 10067
            +++ F    KA ++I++  WS+K  LD  GS G+V+C      Y VGV  +L+ + LTK +TF+P+Y++ N+    +   E    A  W+ + P E  P WPR++   ++++R   +     +T  F F E    L+KLE  K+ GI V+VQ TE    I    Y+   A   LINH G  +  H K   T  E IL A  T+MYAW D    +  ++   G  K+ ++ L  DGI +  + +  K  WVSF+    R+LLFT +  +A     +A L+SI Q I + +H IGLS+++N    ++ Y+ +TSSG+ W   +  KNR K L + ++  LE  + +++  +  S  +V+ + +      +D    ++ K  +  L R F P ++L  ++SP Q  +H++I +IQ+DNQ  D  FPVV AP PPP+SVA  T LKP IE S V      +   ++KY R+LIQE    +D  F+ +I +  +  V        F +D + I    ++  E  +++      +K  +DN H+ P+KIH+SFS++       T      L   +Q VG+ +TD+ DV+F+L +FE++       Q+++ ITSHY  QAL+Q+YVLVLGLDVLGNP+G++ G+ +GVEDLFYEP +GA+ GP EF EG+  GVKSLFGH VGGAAGAVS+I G MGKG+AALT D DYQ+KRR+ +  +P+N   GLA+  KGL++GFV GVTGVVTKP+ GA+ +G+EGFFKG+GKG +G+V RP +GVVDFAS SFE ++R AD + +V R+RPPRF   D ++R Y L EA GN I +K T+K      + ++       K   + +TN R+ ++ RNE+ G+W+  W   + ++    ++  +   + FT+K   +K+ 
Sbjct: 2394 RDKLFFDKKKASIRIDNGQWSEKIPLDVAGSVGEVICFANNQKYPVGVHNHLTQNSLTKQITFIPFYIVCNKCHFDIELQEQSRPADPWLHLEPNEMEPLWPRNDTKNNLVVRVDGK-----ITPAFDFTEVICTLLKLEDSKYGGINVDVQTTEGGVYITFTDYKPADAPGLLINHTGKQIVYHEK--GTKNEHILNAKSTIMYAWDDPTGPKMLVF---GTNKEETD-LKRDGIGEVIMQDGGKVLWVSFLDGLQRVLLFTENESIANRTESTASLQSITQSIDLRIHGIGLSVINNETGLDILYLGVTSSGIIWESKKVTKNRFKELTINENALLEIEYQKYL--VHKSVNDVQTYKLD-NKFPIDFDLMILKKTVERNLRRSFYPAIWLSRKSSPFQSQLHVKINRIQVDNQFLDPIFPVVLAPIPPPKSVASTTSLKPFIECSMVQRIMPNSTVRQFKYARILIQEFLFKVDLNFLTAIAEMFAKEVSDEAAAKQFRQDVESIELPLSAFFEEHSLEE-----QKSFYDNLHLGPLKIHVSFSMA----GSDTKALPGFLGSLVQGVGVTLTDVNDVVFRLAFFEREYQFFSQKQLINEITSHYTGQALKQLYVLVLGLDVLGNPYGLVVGLKKGVEDLFYEPFQGAIQGPGEFAEGLVLGVKSLFGHTVGGAAGAVSKITGAMGKGLAALTFDEDYQKKRRQGIQNKPKNFHEGLARSSKGLVMGFVDGVTGVVTKPVTGARDNGVEGFFKGLGKGAIGLVARPTAGVVDFASGSFEAVKRAADASEDVKRMRPPRFQHYDFVLRPYCLMEATGNKI-MKETDKGKFATTDNFIHCEEIIQKSEYLVVTNYRVMYVQRNEMFGVWTSLWSYLWNEIS---SVAATARGVQFTVKTDGKKVL 3273          

HSP 2 Score: 471.085 bits (1211), Expect = 1.455e-132
Identity = 276/829 (33.29%), Postives = 468/829 (56.45%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIE---ENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTN--FTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRP-NVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFII------SDNSLKEY-KLMKLVIHSMS---AHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKY-TKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRK-LRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPP 2456
            MVFE++V +++NK LGDY+  LD++QLK+G+ GG+  L+NL I+ENA D+ DLPV++  G++ KL LKIPWK+LYS+PVI  ++ LYVL+ PN+ ++Y+ EKE  Y  + K+  L+ +E   + +L+ + PK +      F EKL AQIV NLQ+ I+N+H+RYEDT+T     F+ GI+L  L   T D       + + ++ + FK   +  L+ Y N   + +++ N++ L    K NI+  E K  + ++++ PISC+A + ++M PE  +  F  P++ L L++E++       Q+ ++    D+M+R      +RKYRP N+P + +   WWHF    ILEE+VR+ R  W+W +I+ HR RC  Y   ++++ L KK S    +T ++ E ELD+ N+ L RQ+  ++  K R+ + ++ S      GW G +  +  ++     S +L +K  +  T EEK K+Y+AIGY E     D+P  Y ++++N +L  L+  +      S  + K++ +L  LV+ + S   A + QR +A+AIS+   M  ++VTG    D + P LV ++   +  NLL V FETNPLDK  +Q++ + A PL+I YD+ T+  ++     P ++ L +        I   KE +ATG++++ D++   D+ + + P+  ++P +G Y   +  +L+V +G + + S+              G+D       + E AYD+F + +  +Q L++  G+ W+  L    S++ H+LRP   +     CV+ ND  LP I V   LP + V +S+  +   +++   IP P+   P
Sbjct:    1 MVFEAVVADVLNKVLGDYIENLDRNQLKIGIWGGDVVLQNLKIRENALDELDLPVQLIYGYLGKLVLKIPWKNLYSQPVIVNIEDLYVLVSPNNNVQYNAEKEAKYEMDLKKAALDALEAARKKELEMDQPKADA----GFAEKLTAQIVNNLQVQITNVHLRYEDTTTT-GSPFSFGISLHELELYTTDCDWEKCYMAQQAS-QVFKIANLSCLSAYLNCGGQLYAN-NKSDLSQQFKTNIACKETK-PNYNYVLGPISCNAKLKLNMNPELDDPPFEKPKIDLTLEMEKLNVGLTNTQFDNLMKLGDAMNRQQLGIPYRKYRPYNIPYKGHARDWWHFAITSILEEEVRKPRESWTWGHIKTHRERCNTYAQKYKEQCLSKKPSAVLTETCRLLETELDVFNLLLIRQRVNIEIAKQREAVPEQKSGWFSGWGWGGGAKKDDQTT-----SQKLVEKFEAAMTSEEKEKMYRAIGYQENAKPTDLPESYEAIRMNFKLIALEVGLYKDERNSSAATKDFHELPSLVLLNFSMATALITQRPAAEAISIIAGMREIKVTGLTRNDYT-PLLVESKITDEF-NLLEVFFETNPLDKLCDQRVKVVARPLQITYDAPTILALINAFQTPGDVTLSKFEDAASTKISNFKERSATGMQYMIDKKAVLDVDILLMPNILVVPHKGVYDAGNVSLLVVSMGQVHLSSQPRRESNKLQHLFSAGEDKDEILKTVMENAYDRFTVAVDDVQMLVVRAGEPWQNALAEANSTEMHVLRPVSLKVTAALCVVDNDPRLPNIKVDIDLPAILVNVSEDRIFLAIKVATSIPLPEQKEP 814          

HSP 3 Score: 223.402 bits (568), Expect = 2.503e-57
Identity = 264/1102 (23.96%), Postives = 502/1102 (45.55%), Query Frame = 3
Query: 2961 TKKHTKGQNLLF-INLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMT-QWAITASLQKLRVIVANEKDNNILDISIHGMRAD--VKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKND----FAIDQSTSVVLEESKITLSNLRVSRSILLENT------VKNEKGIL------HPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESI---------TVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKI----------------NELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXM-VPVRGYEKNPRKYRKKP--LAS----PVEPRLAD-----FEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLL---EVYPEEHNRQYS-FEIEYISCDDSGLFD-EEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINK 6080
            T K     N LF ++  I +K+SP+F T + +T   +   F+ +++ LHQE L  ++  +N+    L++V               TR +  +                 SI G   D I     KRT+ +  E   E    +     KK     +  T +  + A+L ++ +++   K   I ++++    +   +K+SY  TEV+I + D+++ D +P  ++K I+ I   ++ F+ Q+ ++N+   T  Y  D    D  + +++G +KIIFL  FV  +  FLN+F  +  T++      +E+A  K  +  E   ++R KL+I +KAP+I +P  S+  NAL++DLG L   N+       ++    V++E K+ + ++++S+ +LL+        V  E G L      +P++  + +TRNLS+     +P+++++ R+ SI + +   DY +  LVL  N NE + + P   E+ +E + R   P+   S + R +    +         +++F F+ + + + L +G+               L +     LSV+G+   N  +  SV L NI + D + +S  +I                +E+I    N+ +  + +  I  K  ++   V + G  + +C+D+L+ LT F         +        PV    ++ +   +    LA+    PVE    +       + + +  P+I+LVE++ + +++ I+      L Y  + +  I+N  I   +   C F  E R+     I+ P  ISL  + P   GM  SL +  +IIN++PA I+ ++K M ++++ +  + +  + S   N       N W +  F    + F                 VE G D     L+ EQR       + DN ++ K  K  + +P I +++ES V     P++  +  +     N    +    +++++++Y+N  +  WEP++   EV      R+Y+ +E+++    + G+   + + ED    E  A+ + + S + LE+T+SK+ L +LS L +AF  A   +      I  APYV++N  G DV   + K
Sbjct:  973 TPKQENSSNYLFTVSCTIADKSSPEFSTKYNSTEQLVVANFEVLQIVLHQECLQRIMEVVNNFQRNLDLV------------LSSTRPRDRM----------------GSIGG--GDGI-----KRTLNVILEDTEEIMTTDQMKRRKKTRRTHVVETVKVRVIANLDQVGLVLTGRK-RPIAEMNVKKFVSSLIIKSSY--TEVNIGLKDIQVLDLNPYTIHKNILSIVGKDA-FNCQIVIYNK-EETQDYNSD----DMKITVDIGCMKIIFLNWFVAGVMNFLNNFTAAQATISQAGAAAAESARQKAMDAYET--ATRMKLNIRIKAPIIIVPIGSQDRNALLLDLGLLELTNNTVEVAVAEEERLAVIDEIKLQICDVKISKIVLLDGNESTVDEVDAEVGFLSKFNMMNPMSCTLSITRNLSYTWYRDVPELNLSGRLKSIELTLFADDYALVMLVLNRNLNEGLEEFPPSEEAPQEAQVR---PERRNSRAGRLSRTVQVSPIREKIHESIKFNFQFDGVVINLMEGEG------------AGLARFGIYFLSVKGTKLDNGTLSTSVVLCNIQMDDMRSNSKSQIRQYLSRKDWVQPKLDTDEIIDACYNERNFMVDVTAII-KEDDTFAEVRVRGFDLIVCIDFLLKLTTFLTLPPEENPRESVYIKPAPVSETARDTKHSIRSSAILAAQELVPVESSSHEVPNRKMNLILHIDEPDIILVENLEDLNTSCIIFNAQVHLNYRSINDKQIVNGQIDALKMYMCAFLPERREMTRHYILHPCVISLQGSTPEEEGMHISLKLSDIIINVSPATIELLNKAMLSVSSGTMTKCAIAEES--RN-----YSNLWHQHHFHSRTYWFTK---------------VEQGVDA----LEAEQRSV-----STDN-EKQKTEKCVIEIPSITLVIESGVGYYTKPLISLDTRITAVFNNWSRSLTAHGSLTLNMNYYNQALAEWEPIIELNEVIGRNGVREYTPWELKF----EMGMEKVQSELED--DAEQQAMHMNIHSAETLEITLSKTCLGLLSELAEAFSQAIDQNGLTKPDIV-APYVLENDTGFDVNLNLRK 1973          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Fly
Match: Vps13D (gene:FBgn0052113 transcript:FBtr0273390)

HSP 1 Score: 130.954 bits (328), Expect = 2.278e-29
Identity = 92/347 (26.51%), Postives = 166/347 (47.84%), Query Frame = 3
Query:   27 VFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDS------FGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWN---SNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTV-----PRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHF 1025
            +   L+  ++N YLG Y+  L+ +QL + LL G   LEN+ I+++A   F+LPV+V  G I K+ L+IP +   + P    ++GL+ +I P +   +D EKE+    E K   L+  E         + E+    S      +G  +A  I+ N+++ IS++H R+ED           GI + +L+ Q+CD   T+   K ++    +K V++  L+VYW+    + K  S  N+  L+ +       S  +L   +F+I+PI  +A      K +     +     PR+S +L + ++    ++ Q   +   L  + +     ++R  RP   + SN   WW +
Sbjct:    1 MLRDLITWVLNTYLGKYLENLNSAQLSVALLSGEVELENIPIRKDALRSFNLPVEVTAGSIRKIKLQIPVRQFRTSPWCISIEGLFCIICPKNLDNWDYEKEKLQDLEYKLAVLDTAEAGWRSEKGKQMESYYFSSYNNWLKYGTNMATNIIDNIELKISDVHFRFEDIVDTGKSKICTGIKIGSLTAQSCDCDWTNGSYKMNNNEMNYKLVELKELSVYWDLLHEDIKCQSYSNQEILEKM------HSTCELRSHNFIIKPICATA----RWKRDKCQQVIRTKDKPRVSCELLVPEVVIDISKVQRLQMLDKLSEIRQVKEVRQYRLKRPTCTVESNPIAWWKY 337          

HSP 2 Score: 58.9214 bits (141), Expect = 1.324e-7
Identity = 111/436 (25.46%), Postives = 197/436 (45.18%), Query Frame = 3
Query: 8571 LRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELS--AVIIK-----SGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVS-GSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLS-FSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVG-LAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVA-DTNIEVF-RIRPPRFI--AKDGIIRSYGLREAEGNNIWIKITEK 9836
            L I  IQIDNQ+ D               +AL T     +E +  AV++K     S    A+ +KY  + ++     L++  +  +  F+  G +   +    +            E +  K  L   K+  F+N  I   ++ LS F+ S++       LH        +++G+ +   +D + +L  +  +     M+     +  HY++Q       ++  +D LGNP G    +++GV  L +E   G+V   +  V+ V +G+ +           + +++  T+   +  + LD D+  + R+++ +   N   G LA G KGL  G + GVT +V    DGA  DG+ GF  G+GKGLVG VT+P+ GV+D AS +   +R  + D++     R R PR +  A  G++  Y  R+++G      I +K
Sbjct: 3411 LNIAYIQIDNQLLDA-----------VSPIALHTQTSNDLEETQNAVVLKLKMLPSPNKNAIIFKYLTLDLKPSTASLEEKLILKVASFLGYGKINRQNLSVQYQ----------FENTDDKPFLQDMKRYYFENLSIGATQVRLSAFTSSKL----PVELHETK-----KALGLTLIKFEDALIELDRYSDKLHFETMDVYRKELKKHYINQVKWHAAAILGSVDFLGNPLGFANDLSEGVSGLIFE---GSV---KSLVKNVTHGISN-----------STAKLTETLSDSLGKVVLD-DHDNETRQRILELQSNTSGGHLAAGLKGLGFGLLGGVTSIVRHTYDGATSDGVPGFLSGLGKGLVGTVTKPIIGVLDLASETASAVRETSRDSHRNAPERKRLPRCVTGAPGGLLPLYSNRQSKGQQYLYLINQK 3798          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Fly
Match: Vps13B (gene:FBgn0039727 transcript:FBtr0085505)

HSP 1 Score: 70.8626 bits (172), Expect = 3.270e-11
Identity = 43/140 (30.71%), Postives = 72/140 (51.43%), Query Frame = 3
Query: 9111 FEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGL 9530
            +E+Q  +    +   S+  HY+S A+     +V  L++LG+P G+ R  + G+ D    PV+G   GP  F+ GV  G  SL  +   G   +V+++ G++ + +  LTLDA++      +   RP+    GL QG  GL
Sbjct: 3353 YERQQILTVPLRFGQSLGLHYLSGAIFGAGWVVGSLEILGSPSGLARSFSTGLRDFVSMPVQGLFRGPWGFLVGVTQGSASLLRNVTAGTVNSVTKLAGSVARNLDRLTLDAEHIELTEARRRARPQGFADGLTQGLTGL 3492          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Zebrafish
Match: vps13c (vacuolar protein sorting 13 homolog C [Source:ZFIN;Acc:ZDB-GENE-070426-4])

HSP 1 Score: 602.823 bits (1553), Expect = 1.781e-172
Identity = 441/1472 (29.96%), Postives = 737/1472 (50.07%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEEN-KLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDK-KLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYT------DIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETA----SLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDAL--FVINTFELQVLQDIKNTLVE--KNGAKE--IVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSI-MGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTS----VVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKIL 4370
            MVFESLV +L+N+++GDYV  LDKSQLK+G+ GG      L +  N   + D+P KV+ G I KLTLKIPWK+LY+E V+A LDGLY+L++P + +KYD  KE  YL E KQKEL RIEE  +L     K + E KD+F EKLA Q++KNLQ+ I++IHIRYED  + P +  A+G+TL+ LS QT D +  + I+ E + +  +K  +++    YWN N   F  ++   +   +K  IS+   +L    ++ +PI  SA M ++   E      P+ +L L+++ I     ++QY  +   L+S+D       +RK+RP+VP+  N   WW +  + ILE  VRR  +MW+W  I+ HR   + Y   ++ KL    K+ +     I+  E+ LD+ NI L RQQ +M+  +  +KL  K ++    SG    S          +  D+  + + S  T EEK+KLY AIGYS  + +  +P  YV++ +N +L        +      +++K+ +  +S  + QR  A AI V   + +  VTG +   G VP+L+++   S   +LL V FE NP D  A+Q + + ++P+EIIYD+ TV  I +F    + + L  I++  L+ +EE+KE T   L H   R I        D+++D+KPSY +LP+ G Y    ++++VD G + + S      G       F      + + + + AY+++ + L ++Q L    G+ W+    +  S  HIL+P  F   L KC++  DA +P+  V G LP L + ISD+ +Q VLEL+  IP P                     Q G +P  + T  +VL     +P S T     +L  T+     E E  +D  E                      +D ++L   +    E+Q   ++K  L E  +  A+E  ++   V ++G    ++ FD++V +Y+  + L   +  D   +    +  + KH  G +LL +  +  +   P F T+  NT   L+ +F S++  LH +ALL  +N++NS IP   +   ++ ET    ++    K  +   ++  + + K      S  + +  DR ++A     +R++           G D+          M Q   T    +LR IV       +LD+                            DP    ++KK + I  +E +FS ++ L+   T   GY  D S+VD  V + +G I+I++L+ F++ +  F+++FQ + + ++    + +E AA+ + +  ++  S R  +DI +KAP+I IP++S S NA +VDLG +T  N F++  +       VLE   I L+ L++SR++L  +    +  IL PIN+ + + RNL+    + IP + +   + S+++ + Q D  +   +L  N  E  ++  +V  +K   K   ++
Sbjct:    1 MVFESLVSDLLNRFIGDYVENLDKSQLKIGIWGGKRIFVKLPV--NISSELDVPFKVKAGQIGKLTLKIPWKNLYNEAVVATLDGLYLLVVPGATIKYDAVKEERYLQEAKQKELQRIEETLQLVARREKPQEEKKDTFAEKLATQVIKNLQVKITSIHIRYEDDVSDPQKPLAMGLTLSELSLQTADENWKTCILNE-AAKIIYKLGRLECFCAYWNVNGPIFYKDSWEGIVEKLKAGISTKNEELQGYQYIFKPIFASARMCINPNAEV-ELKSPKANLHLEVQNIAIEMTKSQYLSMVDLLESIDCMVKNGPYRKFRPDVPVHRNARQWWKYGISSILEVHVRRFNQMWNWTNIKKHRQTLKAYKAAYKVKLTQSAKVREDADKQIQELEKGLDVFNIVLARQQVQMEVVRSGQKLVAKKAATQKQSGGGFFSSFFGKKEGKKEEEDKEPESIDSIMTVEEKSKLYTAIGYSGSSHNLALPKHYVAVIVNFKLLSTSVTFREEP-GVPEILKVQMIDLSTSISQRPGAQAIRVEAKLEHWYVTGLQ-QQGEVPSLIASLGDSD-SSLLSVRFEINPEDSPADQLLRVHSQPVEIIYDALTVISITEFFKTGKGVDLEVITSATLSKLEEIKEKTTVLLSHHPHRHILILRKSVLDLRIDLKPSYLLLPKSGFYDGKSELMIVDFGYLQLNS--VDQGGHQQVSASFS-----SLEEIMDRAYERYSLELRSVQILYSRSGEKWKSACHEGLSHQHILQPMDFTLQLAKCMVDKDARMPRFKVSGELPLLHLKISDQKIQGVLELVDSIPLP---------------------QTGSSPPSSPTGKKVLG----MPLSNTRPKVLALDPTVLSYAVESESDEDTSE--------------------KSVDEESLRSTLEELTEVQFQFEVKAVLFELTRQAAQEDVVLAFSVSQLGAEGRIRTFDLSVTSYLKKISLDYCEEKDVQNQPLHLITSSDKH--GSDLLKVEYIKADVNGPSFCTLFNNTEQTLKVEFSSLDFLLHTKALLSTINYMNSAIP-QELTANKERETRRRADRAAAGKTVSKGSKDAGVVNFKLSAVLGSFRVAVCDDRSNIA----DIRVQ-----------GIDAS--------VMVQAKETDVFARLRDIV-------VLDV----------------------------DPK--TIHKKAVSIVGEE-VFSFKMTLYPGATEGDGYT-DTSKVDGKVIMRLGCIQIVYLHKFLMSLLMFVDNFQTAKEALSAATAQAAEKAASSVRDFAQK--SFRLAMDIRLKAPLIIIPQSSTSHNAFVVDLGLITVGNRFSLLAAEGFPLPAVLETMDIKLTQLKLSRTVLKHDADHLDIEILEPINLELLVKRNLAAAWYNKIPGVEVNGVLMSMNMSLGQEDLGVLMRILAENIGEG-SKAAAVEGTKASAKDEAVI 1345          

HSP 2 Score: 524.242 bits (1349), Expect = 1.553e-148
Identity = 351/894 (39.26%), Postives = 535/894 (59.84%), Query Frame = 3
Query: 7368 KNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG----WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSN--NLTSDGIEDF-YINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSK--KLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEA---MFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA--LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFH-VYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPP 10004
            K   F+K  K  L ++ S WS+ FSLDTVGS G V C  K   Y VGV I +SS  LT+IVT  P+Y ++N+S   L   E+  N G    W  +   E +PFWP S   K  +     E      ++ F F +  S  +    ++ G+ V+V I++ + +I+   Y +G A   ++NH      ++F+Q    +   L   +   +AW D    R+  W  +     HS   +L  D    F Y + ++ +WVSF+    R+L+FT D+ +   AR + +LE   QE+ +SL ++GLSL++N ++QE+AY+ ITSSGV W ++R KNR K         LE A+   +N+       +E       G+EV+  +   LM +P  C++ R F PG+ ++ + SPHQ  +  ++Y +Q+DNQ+    FP VF P P P+S+  D+  KP I++S +   +  +  M++KYF  L+QEM + +DQGF+ +IL   + +  + D +    +  KD   ++A L+E+S   T  SG     F++FHISPIK+HLS SL       +    +  + +NL L+S+G  +TD+ D+IFKL YFE +      ++++ ++  HY  Q L QMYVLVLGLDVLGNPFG+IRG+++GVE  FYEP++GAV GPEEF EG+  GV+SL GH VGGAAG VSRI G++GKG+AA+T+D +YQ+KRRE++ +  ++ G  LA+GGKG + G V GVTG+VTKP++GAKK+G  GFFKG+GKGLVGVV RP  G+VD ASS+F+GI+RVA++  EV ++RP R I +DGIIR Y    +EG +++ +   +   +  E++  H +Y   +K  I +TN+R+  +   +++G ++ +W+  +E    PP
Sbjct: 2752 KKNIFSKN-KLQLCVSTSSWSEGFSLDTVGSYGCVRCPDKSGDYLVGVSIQMSSFNLTRIVTMSPFYTLVNKSLFELEVGEVQNNNGSSNKWHYISSTECLPFWPESSTEKLCVRVVGCESS----SKSFFFNKQDSGTLLRMDQYGGVIVDVNISDHSTVISFTDYYDGAAPALVVNHT-PSATVNFRQRGCEECWALRPGEAQRFAWDDPAGVRKLCWSCQ----DHSGELDLVKDECGQFAYSSLVQIHWVSFLDGRQRVLIFTEDVGIVTKARQAEELEQFQQEVNVSLQNLGLSLINNDIRQEIAYVGITSSGVVW-EMRPKNRWKSFNHKNIGLLEKAYQNHINKSDPGWTMLET------GIEVNFGRVPMLMRQPFSCQIRRNFLPGIHVELKQSPHQRSLRAQLYWLQVDNQLPGAIFPTVFHPVPLPKSIVQDSEPKPFIDVSIITRFNEHSQVMQFKYFMALVQEMAVKIDQGFLGAILALFTPATDSLDSKKKNKLIQKDLDALQAQLMESSLTDT--SG--LSFFEHFHISPIKLHLSLSLGSSGDESEQGDMVAIQSVNLLLKSIGATLTDVDDLIFKLAYFEVKYQFYRRDKLMWAVIRHYTEQFLTQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPIQGAVQGPEEFAEGLVIGVRSLLGHTVGGAAGMVSRITGSVGKGLAAITMDKEYQQKRREEMNRPTKDFGESLAKGGKGFLKGVVGGVTGIVTKPVEGAKKEGAAGFFKGIGKGLVGVVARPTGGIVDMASSTFQGIQRVAESTEEVTKLRPVRLIHEDGIIRPYDHHASEGYDLFQR--SEIKQLDGEIFREHYMYPGHRKTNIMITNRRVMCVKEIDVIGHFNKEWECQFESFQRPP 3622          

HSP 3 Score: 309.301 bits (791), Expect = 3.292e-83
Identity = 346/1512 (22.88%), Postives = 670/1512 (44.31%), Query Frame = 3
Query: 2796 KNGAKE-IVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESI-----------------TVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINE--LIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXX--XXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKE--SDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDF--EDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVY-LKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVS---EREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSD--------KSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLC---KSNTGVMYYYSVALSIEEVQIVNSSETL--RTYKLDINVPLALHNQLPIKISY--QSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAV 7184
            KNG +   + L V ++G    ++++DM    YI ++ +   +F D S   P+C+  +   + G +LL +     ++  P+F +++ NT  K+   F S+++ LH EALL  ++F+++ +          T +  SP           E+E++  ED+K                       T   K+ A+S  SD +  D                +   L    V+V ++K +N+ DI I G+   ++     T +   + D  + +     ++KK I I  DE +FS  ++L  + T   GY  D S+VD  V + VG I++++L+ F + +  F N+FQE+ + ++    + +E AA+ + +  ++  S R  +DI +KAP+I IP++S S NA+  DLG +T  N F++     +    V++   I L+ L++SR  +  ++ +    +L P+N+ + + RNL+ +    +  I +   +  + V +SQ D ++   +L  N  EA            +++S   L +++    ARTA+   I                 +V+F F +E L + L+  D +      E     CL +     + + G M  N  MEVS  L+    LD +    +++    L R  +  S   I + + Q  +  S + V +  +Y+C  V++L+ + DFFV+A P     Q  K   +P++ Y   P K   +P A P +      ++   V  PE++ V +++  D+ A++   +F   +S++ E     +  ++   + + CPF      K    ++ P S+ L    P N  + GS+T+E +I+ I+P ++ ++  I   +  +  +E S +      + DQ   ++ W         + F+               GV++  +V +   DV+  +  + F               L +  ++V LES +  + VP+L+ E S     +N  + + +   +++ V+YFN+   VWEPLLE   +   R+++ +++     ++ + D+     +D I +     +I + S+D + +TVSK  L+V +NL KAF ++ +   + H     +P+ ++N  G  V   +    +L+   S    KV+ L  D  + L  +  E+ S  +  ++ +   F          LSI  +    +   P    G    NV    + E  ++++   A  G K + +RS LQ+ N+    +    L+  S+         +E  K   +  E    + + +P+  L     K+Y          S +   W    +       +L C   +SN   +   ++A+  +   I N  E      Y + ++ P+ + N LP  I Y  +     ++++ G    V  + +  +    +   YQ + W G + +   M E   I F    G + + +D+ VH++  +G+  ++++SPYW++NKT   L Y+A +D  V+H +   D +
Sbjct: 1392 KNGDESPFLVLDVSQLGIDTKVRKYDMEATTYIKTISMKCLEFPD-SNGEPLCIISSSAES-GADLLEVKYFKADRNGPNFASLYQNTEQKMNVVFSSLDLMLHTEALLSTMDFLSAAL---------STSSLPSP-----------ERESKKSEDMK-----------------------TTSAKSTALSSPSDGDIID--------------LMVNMQLGAFNVLVCDQK-SNMADIKIQGLDGSLQMKGTQTHMFTRLRDFIVINVDSKTIHKKAISIVGDE-VFSFSMSLTPKATEGAGYT-DTSKVDGKVKLNVGCIQVVYLHKFFMSLLNFSNNFQEAKEALSAATAQAAEKAASSVRDFAQK--SFRLAMDIKLKAPLIIIPQSSTSHNAVEADLGLITVGNRFSLLPVEGRPLPAVIDNMDIQLTQLKLSRISMELDSDQTSSKLLEPVNLVLSVKRNLASSWYQKMAAIEVDGDLKPMKVALSQDDLSVLLRILMENIGEA-----------SDIQSDTALRQENVLVRARTATGSEILEEKLSESKLKETELLESVRFSFNVESLGLTLYNNDPSQPCVHEE---KSCLGEFMLCKMKMSGKMLNNGNMEVSTILTTCT-LDDRRSGIQRVTSRMLGRRDEETSEPMIDVTYSQSVDERSVVAV-LQKLYLCASVEFLLAVADFFVQALPTSPPTQREKTNQLPLK-YVSEP-KIHTEPKAVPAQRT----KLRAVVVDPEVVFVANLMKADAPALV--ASFQCDFSLLSEEAGQTMKANVRSLKVLACPFIRAEGDKAVTTVLRPCSVELETKMPANAPLAGSMTVEEVIVKISPIILNTVITITAAMAPKPKEELSQQ-----KSGDQ---NSLWSLMSVANANYWFL---------------GVDTASEVTESFTDVDSSKDGETFG--------------LTVKVVQVTLESGLGHRTVPLLLAESSFSVTAQNWSSLLSVSADMTLEVNYFNETHAVWEPLLERV-DNGKRRWNLKLDM---KNNPVRDKSPVPGDDFIMLPDPRTAITICSKDTMNITVSKCCLNVFNNLAKAFSESTASTFD-HSLKEKSPFTIRNALG--VALIVQHSANLRVPGSSTLGKVHELAVDKSLDLEYSSFERSSRQSALQRQESCLFN---------LSIVPSGYSEISNIPLGKPGRRLYNVRQPMQEESVSVLLQIDASEGNKVITVRSPLQIKNHFS--VPFAILKYSSELGGMVNVGVAEPEKECHVPLEAYRSQLFLLPVGPL----EKLY--------RASTTCIAWKEKVHVSSEVHAVLQCPATESNFLPLVVNTLAMPDDLSHIANHGEVEWDPAYLIHLHPPVTVKNLLPYSIRYLLEGSADIYELQEGSAADVLNSNVSGEIMELILIKYQGRNWTGRMRISRDMPEFITICFNCDTGEALT-VDVCVHVSRVSGKFILSVHSPYWIINKTSRVLQYRA-EDTFVKHPSDFRDVI 2746          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Zebrafish
Match: vps13c (vacuolar protein sorting 13 homolog C [Source:ZFIN;Acc:ZDB-GENE-070426-4])

HSP 1 Score: 583.948 bits (1504), Expect = 9.382e-167
Identity = 444/1513 (29.35%), Postives = 740/1513 (48.91%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNS-----------EMKYDPEKERSYLFETKQKELNRIEE--------------------NKLKRNDP-----------KKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDK-KLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYT------DIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETA----SLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDAL--FVINTFELQVLQDIKNTLVE--KNGAKEIVKLI--VHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSI-MGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTS----VVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKIL 4370
            MVFESLV +L+N+++GDYV  LDKSQLK+G+ GG      L +  N   + D+P KV+ G I KLTLKIPWK+LY+E V+A LDGLY+L++P +            +KYD  KE  YL E KQKEL RIEE                    + + +N P           K + E KD+F EKLA Q++KNLQ+ I++IHIRYED  + P +  A+G+TL+ LS QT D +  + I+ E + +  +K  +++    YWN N   F  ++   +   +K  IS+   +L    ++ +PI  SA M ++   E      P+ +L L+++ I     ++QY  +   L+S+D       +RK+RP+VP+  N   WW +  + ILE  VRR  +MW+W  I+ HR   + Y   ++ KL    K+ +     I+  E+ LD+ NI L RQQ +M+  +  +KL  K ++    SG    S          +  D+  + + S  T EEK+KLY AIGYS  + +  +P  YV++ +N +L        +      +++K+ +  +S  + QR  A AI V   + +  VTG +   G VP+L+++   S   +LL V FE NP D  A+Q + + ++P+EIIYD+ TV  I +F    + + L  I++  L+ +EE+KE T   L H   R I        D+++D+KPSY +LP+ G Y    ++++VD G + + S      G       F      + + + + AY+++ + L ++Q L    G+ W+    +  S  HIL+P  F   L KC++  DA +P+  V G LP L + ISD+ +Q VLEL+  IP P                     Q G +P  + T  +VL     +P S T     +L  T+     E E  +D  E                      +D ++L   +    E+Q   ++K  L E  +  A+E V L   V ++G    ++ FD++V +Y+  + L   +  D   +    +  + KH  G +LL +  +  +   P F T+  NT   L+ +F S++  LH +ALL  +N++NS IP   +   ++ ET    ++    K  +   ++  + + K      S  + +  DR ++A     +R++           G D+          M Q   T    +LR IV       +LD+                            DP    ++KK + I  +E +FS ++ L+   T   GY  D S+VD  V + +G I+I++L+ F++ +  F+++FQ + + ++    + +E AA+ + +  ++  S R  +DI +KAP+I IP++S S NA +VDLG +T  N F++  +       VLE   I L+ L++SR++L  +    +  IL PIN+ + + RNL+    + IP + +   + S+++ + Q D  +   +L  N  E  ++  +V  +K   K   ++
Sbjct:    1 MVFESLVSDLLNRFIGDYVENLDKSQLKIGIWGGKRIFVKLPV--NISSELDVPFKVKAGQIGKLTLKIPWKNLYNEAVVATLDGLYLLVVPGAIESKLFSSSYNAIKYDAVKEERYLQEAKQKELQRIEETLQLVARRDSQAGEFVFNLESYVYKNPPRGMYRPIAIEEKPQEEKKDTFAEKLATQVIKNLQVKITSIHIRYEDDVSDPQKPLAMGLTLSELSLQTADENWKTCILNE-AAKIIYKLGRLECFCAYWNVNGPIFYKDSWEGIVEKLKAGISTKNEELQGYQYIFKPIFASARMCINPNAEV-ELKSPKANLHLEVQNIAIEMTKSQYLSMVDLLESIDCMVKNGPYRKFRPDVPVHRNARQWWKYGISSILEVHVRRFNQMWNWTNIKKHRQTLKAYKAAYKVKLTQSAKVREDADKQIQELEKGLDVFNIVLARQQVQMEVVRSGQKLVAKKAATQKQSGGGFFSSFFGKKEGKKEEEDKEPESIDSIMTVEEKSKLYTAIGYSGSSHNLALPKHYVAVIVNFKLLSTSVTFREEP-GVPEILKVQMIDLSTSISQRPGAQAIRVEAKLEHWYVTGLQ-QQGEVPSLIASLGDSD-SSLLSVRFEINPEDSPADQLLRVHSQPVEIIYDALTVISITEFFKTGKGVDLEVITSATLSKLEEIKEKTTVLLSHHPHRHILILRKSVLDLRIDLKPSYLLLPKSGFYDGKSELMIVDFGYLQLNS--VDQGGHQQVSASFS-----SLEEIMDRAYERYSLELRSVQILYSRSGEKWKSACHEGLSHQHILQPMDFTLQLAKCMVDKDARMPRFKVSGELPLLHLKISDQKIQGVLELVDSIPLP---------------------QTGSSPPSSPTGKKVLG----MPLSNTRPKVLALDPTVLSYAVESESDEDTSE--------------------KSVDEESLRSTLEELTEVQFQFEVKAVLFELTRQAAQEDVVLAFSVSQLGAEGRIRTFDLSVTSYLKKISLDYCEEKDVQNQPLHLITSSDKH--GSDLLKVEYIKADVNGPSFCTLFNNTEQTLKVEFSSLDFLLHTKALLSTINYMNSAIP-QELTANKERETRRRADRAAAGKTVSKGSKDAGVVNFKLSAVLGSFRVAVCDDRSNIA----DIRVQ-----------GIDAS--------VMVQAKETDVFARLRDIV-------VLDV----------------------------DPK--TIHKKAVSIVGEE-VFSFKMTLYPGATEGDGYT-DTSKVDGKVIMRLGCIQIVYLHKFLMSLLMFVDNFQTAKEALSAATAQAAEKAASSVRDFAQK--SFRLAMDIRLKAPLIIIPQSSTSHNAFVVDLGLITVGNRFSLLAAEGFPLPAVLETMDIKLTQLKLSRTVLKHDADHLDIEILEPINLELLVKRNLAAAWYNKIPGVEVNGVLMSMNMSLGQEDLGVLMRILAENIGEG-SKAAAVEGTKASAKDEAVI 1386          

HSP 2 Score: 504.212 bits (1297), Expect = 2.166e-142
Identity = 338/831 (40.67%), Postives = 503/831 (60.53%), Query Frame = 3
Query: 7368 KNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG----WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSN--NLTSDGIEDF-YINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSK--KLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEA---MFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA--LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIW 9818
            K   F+K  K  L ++ S WS+ FSLDTVGS G V C  K   Y VGV I +SS  LT+IVT  P+Y ++N+S   L   E+  N G    W  +   E +PFWP S   K  +     E      ++ F F +  S  +    ++ G+ V+V I++ + +I+   Y +G A   ++NH      ++F+Q    +   L   +   +AW D    R+  W  +     HS   +L  D    F Y + ++ +WVSF+    R+L+FT D+ +   AR + +LE   QE+ +SL ++GLSL++N ++QE+AY+ ITSSGV W ++R KNR K         LE A+   +N+       +E       G+EV+  +   LM +P  C++ R F PG+ ++ + SPHQ  +  ++Y +Q+DNQ+    FP VF P P P+S+  D+  KP I++S +   +  +  M++KYF  L+QEM + +DQGF+ +IL   + +  + D +    +  KD   ++A L+E+S   T  SG     F++FHISPIK+HLS SL       +    +  + +NL L+S+G  +TD+ D+IFKL YFE +      ++++ ++  HY  Q L QMYVLVLGLDVLGNPFG+IRG+++GVE  FYEP++GAV GPEEF EG+  GV+SL GH VGGAAG VSRI G++GKG+AA+T+D +YQ+KRRE++ +  ++ G  LA+GGKG + G V GVTG+VTKP++GAKK+G  GFFKG+GKGLVGVV RP  G+VD ASS+F+GI+RVA++  EV ++RP R I +DGIIR Y    +EG +++
Sbjct: 2793 KKNIFSKN-KLQLCVSTSSWSEGFSLDTVGSYGCVRCPDKSGDYLVGVSIQMSSFNLTRIVTMSPFYTLVNKSLFELEVGEVQNNNGSSNKWHYISSTECLPFWPESSTEKLCVRVVGCESS----SKSFFFNKQDSGTLLRMDQYGGVIVDVNISDHSTVISFTDYYDGAAPALVVNHT-PSATVNFRQRGCEECWALRPGEAQRFAWDDPAGVRKLCWSCQ----DHSGELDLVKDECGQFAYSSLVQIHWVSFLDGRQRVLIFTEDVGIVTKARQAEELEQFQQEVNVSLQNLGLSLINNDIRQEIAYVGITSSGVVW-EMRPKNRWKSFNHKNIGLLEKAYQNHINKSDPGWTMLET------GIEVNFGRVPMLMRQPFSCQIRRNFLPGIHVELKQSPHQRSLRAQLYWLQVDNQLPGAIFPTVFHPVPLPKSIVQDSEPKPFIDVSIITRFNEHSQVMQFKYFMALVQEMAVKIDQGFLGAILALFTPATDSLDSKKKNKLIQKDLDALQAQLMESSLTDT--SG--LSFFEHFHISPIKLHLSLSLGSSGDESEQGDMVAIQSVNLLLKSIGATLTDVDDLIFKLAYFEVKYQFYRRDKLMWAVIRHYTEQFLTQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPIQGAVQGPEEFAEGLVIGVRSLLGHTVGGAAGMVSRITGSVGKGLAAITMDKEYQQKRREEMNRPTKDFGESLAKGGKGFLKGVVGGVTGIVTKPVEGAKKEGAAGFFKGIGKGLVGVVARPTGGIVDMASSTFQGIQRVAESTEEVTKLRPVRLIHEDGIIRPYDHHASEGYDLF 3602          

HSP 3 Score: 308.916 bits (790), Expect = 4.310e-83
Identity = 346/1512 (22.88%), Postives = 670/1512 (44.31%), Query Frame = 3
Query: 2796 KNGAKE-IVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESI-----------------TVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINE--LIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXX--XXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKE--SDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDF--EDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVY-LKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVS---EREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSD--------KSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLC---KSNTGVMYYYSVALSIEEVQIVNSSETL--RTYKLDINVPLALHNQLPIKISY--QSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAV 7184
            KNG +   + L V ++G    ++++DM    YI ++ +   +F D S   P+C+  +   + G +LL +     ++  P+F +++ NT  K+   F S+++ LH EALL  ++F+++ +          T +  SP           E+E++  ED+K                       T   K+ A+S  SD +  D                +   L    V+V ++K +N+ DI I G+   ++     T +   + D  + +     ++KK I I  DE +FS  ++L  + T   GY  D S+VD  V + VG I++++L+ F + +  F N+FQE+ + ++    + +E AA+ + +  ++  S R  +DI +KAP+I IP++S S NA+  DLG +T  N F++     +    V++   I L+ L++SR  +  ++ +    +L P+N+ + + RNL+ +    +  I +   +  + V +SQ D ++   +L  N  EA            +++S   L +++    ARTA+   I                 +V+F F +E L + L+  D +      E     CL +     + + G M  N  MEVS  L+    LD +    +++    L R  +  S   I + + Q  +  S + V +  +Y+C  V++L+ + DFFV+A P     Q  K   +P++ Y   P K   +P A P +      ++   V  PE++ V +++  D+ A++   +F   +S++ E     +  ++   + + CPF      K    ++ P S+ L    P N  + GS+T+E +I+ I+P ++ ++  I   +  +  +E S +      + DQ   ++ W         + F+               GV++  +V +   DV+  +  + F               L +  ++V LES +  + VP+L+ E S     +N  + + +   +++ V+YFN+   VWEPLLE   +   R+++ +++     ++ + D+     +D I +     +I + S+D + +TVSK  L+V +NL KAF ++ +   + H     +P+ ++N  G  V   +    +L+   S    KV+ L  D  + L  +  E+ S  +  ++ +   F          LSI  +    +   P    G    NV    + E  ++++   A  G K + +RS LQ+ N+    +    L+  S+         +E  K   +  E    + + +P+  L     K+Y          S +   W    +       +L C   +SN   +   ++A+  +   I N  E      Y + ++ P+ + N LP  I Y  +     ++++ G    V  + +  +    +   YQ + W G + +   M E   I F    G + + +D+ VH++  +G+  ++++SPYW++NKT   L Y+A +D  V+H +   D +
Sbjct: 1433 KNGDESPFLVLDVSQLGIDTKVRKYDMEATTYIKTISMKCLEFPD-SNGEPLCIISSSAES-GADLLEVKYFKADRNGPNFASLYQNTEQKMNVVFSSLDLMLHTEALLSTMDFLSAAL---------STSSLPSP-----------ERESKKSEDMK-----------------------TTSAKSTALSSPSDGDIID--------------LMVNMQLGAFNVLVCDQK-SNMADIKIQGLDGSLQMKGTQTHMFTRLRDFIVINVDSKTIHKKAISIVGDE-VFSFSMSLTPKATEGAGYT-DTSKVDGKVKLNVGCIQVVYLHKFFMSLLNFSNNFQEAKEALSAATAQAAEKAASSVRDFAQK--SFRLAMDIKLKAPLIIIPQSSTSHNAVEADLGLITVGNRFSLLPVEGRPLPAVIDNMDIQLTQLKLSRISMELDSDQTSSKLLEPVNLVLSVKRNLASSWYQKMAAIEVDGDLKPMKVALSQDDLSVLLRILMENIGEA-----------SDIQSDTALRQENVLVRARTATGSEILEEKLSESKLKETELLESVRFSFNVESLGLTLYNNDPSQPCVHEE---KSCLGEFMLCKMKMSGKMLNNGNMEVSTILTTCT-LDDRRSGIQRVTSRMLGRRDEETSEPMIDVTYSQSVDERSVVAV-LQKLYLCASVEFLLAVADFFVQALPTSPPTQREKTNQLPLK-YVSEP-KIHTEPKAVPAQRT----KLRAVVVDPEVVFVANLMKADAPALV--ASFQCDFSLLSEEAGQTMKANVRSLKVLACPFIRAEGDKAVTTVLRPCSVELETKMPANAPLAGSMTVEEVIVKISPIILNTVITITAAMAPKPKEELSQQ-----KSGDQ---NSLWSLMSVANANYWFL---------------GVDTASEVTESFTDVDSSKDGETFG--------------LTVKVVQVTLESGLGHRTVPLLLAESSFSVTAQNWSSLLSVSADMTLEVNYFNETHAVWEPLLERV-DNGKRRWNLKLDM---KNNPVRDKSPVPGDDFIMLPDPRTAITICSKDTMNITVSKCCLNVFNNLAKAFSESTASTFD-HSLKEKSPFTIRNALG--VALIVQHSANLRVPGSSTLGKVHELAVDKSLDLEYSSFERSSRQSALQRQESCLFN---------LSIVPSGYSEISNIPLGKPGRRLYNVRQPMQEESVSVLLQIDASEGNKVITVRSPLQIKNHFS--VPFAILKYSSELGGMVNVGVAEPEKECHVPLEAYRSQLFLLPVGPL----EKLY--------RASTTCIAWKEKVHVSSEVHAVLQCPATESNFLPLVVNTLAMPDDLSHIANHGEVEWDPAYLIHLHPPVTVKNLLPYSIRYLLEGSADIYELQEGSAADVLNSNVSGEIMELILIKYQGRNWTGRMRISRDMPEFITICFNCDTGEALT-VDVCVHVSRVSGKFILSVHSPYWIINKTSRVLQYRA-EDTFVKHPSDFRDVI 2787          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Zebrafish
Match: vps13c (vacuolar protein sorting 13 homolog C [Source:ZFIN;Acc:ZDB-GENE-070426-4])

HSP 1 Score: 524.628 bits (1350), Expect = 1.173e-148
Identity = 351/894 (39.26%), Postives = 535/894 (59.84%), Query Frame = 3
Query: 7368 KNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG----WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSN--NLTSDGIEDF-YINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSK--KLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEA---MFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA--LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFH-VYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPP 10004
            K   F+K  K  L ++ S WS+ FSLDTVGS G V C  K   Y VGV I +SS  LT+IVT  P+Y ++N+S   L   E+  N G    W  +   E +PFWP S   K  +     E      ++ F F +  S  +    ++ G+ V+V I++ + +I+   Y +G A   ++NH      ++F+Q    +   L   +   +AW D    R+  W  +     HS   +L  D    F Y + ++ +WVSF+    R+L+FT D+ +   AR + +LE   QE+ +SL ++GLSL++N ++QE+AY+ ITSSGV W ++R KNR K         LE A+   +N+       +E       G+EV+  +   LM +P  C++ R F PG+ ++ + SPHQ  +  ++Y +Q+DNQ+    FP VF P P P+S+  D+  KP I++S +   +  +  M++KYF  L+QEM + +DQGF+ +IL   + +  + D +    +  KD   ++A L+E+S   T  SG     F++FHISPIK+HLS SL       +    +  + +NL L+S+G  +TD+ D+IFKL YFE +      ++++ ++  HY  Q L QMYVLVLGLDVLGNPFG+IRG+++GVE  FYEP++GAV GPEEF EG+  GV+SL GH VGGAAG VSRI G++GKG+AA+T+D +YQ+KRRE++ +  ++ G  LA+GGKG + G V GVTG+VTKP++GAKK+G  GFFKG+GKGLVGVV RP  G+VD ASS+F+GI+RVA++  EV ++RP R I +DGIIR Y    +EG +++ +   +   +  E++  H +Y   +K  I +TN+R+  +   +++G ++ +W+  +E    PP
Sbjct: 2748 KKNIFSKN-KLQLCVSTSSWSEGFSLDTVGSYGCVRCPDKSGDYLVGVSIQMSSFNLTRIVTMSPFYTLVNKSLFELEVGEVQNNNGSSNKWHYISSTECLPFWPESSTEKLCVRVVGCESS----SKSFFFNKQDSGTLLRMDQYGGVIVDVNISDHSTVISFTDYYDGAAPALVVNHT-PSATVNFRQRGCEECWALRPGEAQRFAWDDPAGVRKLCWSCQ----DHSGELDLVKDECGQFAYSSLVQIHWVSFLDGRQRVLIFTEDVGIVTKARQAEELEQFQQEVNVSLQNLGLSLINNDIRQEIAYVGITSSGVVW-EMRPKNRWKSFNHKNIGLLEKAYQNHINKSDPGWTMLET------GIEVNFGRVPMLMRQPFSCQIRRNFLPGIHVELKQSPHQRSLRAQLYWLQVDNQLPGAIFPTVFHPVPLPKSIVQDSEPKPFIDVSIITRFNEHSQVMQFKYFMALVQEMAVKIDQGFLGAILALFTPATDSLDSKKKNKLIQKDLDALQAQLMESSLTDT--SG--LSFFEHFHISPIKLHLSLSLGSSGDESEQGDMVAIQSVNLLLKSIGATLTDVDDLIFKLAYFEVKYQFYRRDKLMWAVIRHYTEQFLTQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPIQGAVQGPEEFAEGLVIGVRSLLGHTVGGAAGMVSRITGSVGKGLAAITMDKEYQQKRREEMNRPTKDFGESLAKGGKGFLKGVVGGVTGIVTKPVEGAKKEGAAGFFKGIGKGLVGVVARPTGGIVDMASSTFQGIQRVAESTEEVTKLRPVRLIHEDGIIRPYDHHASEGYDLFQR--SEIKQLDGEIFREHYMYPGHRKTNIMITNRRVMCVKEIDVIGHFNKEWECQFESFQRPP 3618          

HSP 2 Score: 520.005 bits (1338), Expect = 3.462e-147
Identity = 295/802 (36.78%), Postives = 460/802 (57.36%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEEN-KLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDK-KLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELI 2423
            MVFESLV +L+N+++GDYV  LDKSQLK+G+ GGN  LENL +KENA  + D+P KV+ G I KLTLKIPWK+LY+E V+A LDGLY+L++P + +KYD  KE  YL E KQKEL RIEE  +L     K + E KD+F EKLA Q++KNLQ+ I++IHIRYED  + P +  A+G+TL+ LS QT D +  + I+ E + +  +K  +++    YWN N   F  ++   +   +K  IS+   +L    ++ +PI  SA M ++   E      P+ +L L+++ I     ++QY  +   L+S+D       +RK+RP+VP+  N   WW +  + ILE  VRR  +MW+W  I+ HR   + Y   ++ KL    K+ +  +  I+  E+ LD+ NI L RQQ +M+  +  +KL  K ++    SG    S          +  D+  + + S  T EEK+KLY AIGYS  + +  +P  YV++ +N +L        +      +++K+ +  +S  + QR  A AI V   + +  VTG +   G VP+L+++   S   +LL V FE NP D  A+Q + + ++P+EIIYD+ TV  I +F    + + L  I++  L+ +EE+KE TATGL H+ + R   D+++D+KPSY +LP+ G Y    ++++VD G + + S      G       F      + + + + AY+++ + L ++Q L    G+ W+    +  S  HIL+P  F   L KC++  DA +P+  V G LP L + ISD+ +Q VLEL+
Sbjct:    1 MVFESLVSDLLNRFIGDYVENLDKSQLKIGIWGGNVVLENLKVKENALSELDVPFKVKAGQIGKLTLKIPWKNLYNEAVVATLDGLYLLVVPGATIKYDAVKEERYLQEAKQKELQRIEETLQLVARREKPQEEKKDTFAEKLATQVIKNLQVKITSIHIRYEDDVSDPQKPLAMGLTLSELSLQTADENWKTCILNE-AAKIIYKLGRLECFCAYWNVNGPIFYKDSWEGIVEKLKAGISTKNEELQGYQYIFKPIFASARMCINPNAE-VELKSPKANLHLEVQNIAIEMTKSQYLSMVDLLESIDCMVKNGPYRKFRPDVPVHRNARQWWKYGISSILEVHVRRFNQMWNWTNIKKHRQTLKSYKAAYKVKLTQSAKVREDTEKQIQELEKGLDVFNIVLARQQVQMEVVRSGQKLVAKKAATQKQSGGGFFSSFFGKKEGKKEEEDKEPESIDSIMTVEEKSKLYTAIGYSGSSHNLALPKHYVAVIVNFKLLSTSVTFREEP-GVPEILKVQMIDLSTSISQRPGAQAIRVEAKLEHWYVTGLQ-QQGEVPSLIASLGDSN-SSLLSVRFEINPEDSPADQLLRVHSQPVEIIYDALTVISITEFFKTGKGVDLEVITSATLSKLEEIKEKTATGLSHIIETRKVLDLRIDLKPSYLLLPKSGFYDGKSELMIVDFGYLQLNS--VDQGGHQQVSASFS-----SLEEIMDRAYERYSLELRSVQILYSRSGEKWKSACHEGLSHQHILQPMDFTLQLAKCMVDKDARMPRFKVSGELPLLHLKISDQKIQGVLELV 790          

HSP 3 Score: 308.916 bits (790), Expect = 3.547e-83
Identity = 346/1512 (22.88%), Postives = 669/1512 (44.25%), Query Frame = 3
Query: 2796 KNGAKE-IVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESI-----------------TVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINE--LIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXX--XXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKE--SDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDF--EDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVY-LKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVS---EREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSD--------KSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLC---KSNTGVMYYYSVALSIEEVQIVNSSETL--RTYKLDINVPLALHNQLPIKISY--QSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAV 7184
            KNG +   + L V ++G    ++++DM    YI ++ +   +F D S   P+C+  +   + G +LL +     ++  P+F +++ NT  K+   F S+++ LH EALL  ++F+++ +          T +  SP           E+E++  ED+K                       T   K+ A+S  SD +  D                +   L    V+V ++K +N+ DI I G+   ++     T +   + D  + +     ++KK I I  DE +FS  ++L  + T   GY  D S+VD  V + VG I++++L+ F + +  F N+FQE+ + ++    + +E AA+ + +  ++  S R  +DI +KAP+I IP++S S NA+  DLG +T  N F++     +    V++   I L+ L++SR  +  ++ +    +L P+N+ + + RNL+ +    +  I +   +  + V +SQ D ++   +L  N  EA            +++S   L +++    ARTA+   I                 +V+F F +E L + L+  D +      E     CL +     + + G M  N  MEVS  L+    LD +    +++    L R  +  S   I + + Q  +  S + V +  +Y+C  V++L+ + DFFV+A P     Q  K   +P++ Y   P K   +P A P +      ++   V  PE++ V +++  D+ A++   +F   +S++ E     +  ++   + + CPF      K    ++ P S+ L    P N  + GS+T+E +I+ I+P ++ ++  I   +  +  +E S +        DQ   ++ W         + F+               GV++  +V +   DV+  +  + F               L +  ++V LES +  + VP+L+ E S     +N  + + +   +++ V+YFN+   VWEPLLE   +   R+++ +++     ++ + D+     +D I +     +I + S+D + +TVSK  L+V +NL KAF ++ +   + H     +P+ ++N  G  V   +    +L+   S    KV+ L  D  + L  +  E+ S  +  ++ +   F          LSI  +    +   P    G    NV    + E  ++++   A  G K + +RS LQ+ N+    +    L+  S+         +E  K   +  E    + + +P+  L     K+Y          S +   W    +       +L C   +SN   +   ++A+  +   I N  E      Y + ++ P+ + N LP  I Y  +     ++++ G    V  + +  +    +   YQ + W G + +   M E   I F    G + + +D+ VH++  +G+  ++++SPYW++NKT   L Y+A +D  V+H +   D +
Sbjct: 1388 KNGDESPFLVLDVSQLGIDTKVRKYDMEATTYIKTISMKCLEFPD-SNGEPLCIISSSAES-GADLLEVKYFKADRNGPNFASLYQNTEQKMNVVFSSLDLMLHTEALLSTMDFLSAAL---------STSSLPSP-----------ERESKKSEDMK-----------------------TTSAKSTALSSPSDGDIID--------------LMVNMQLGAFNVLVCDQK-SNMADIKIQGLDGSLQMKGTQTHMFTRLRDFIVINVDSKTIHKKAISIVGDE-VFSFSMSLTPKATEGAGYT-DTSKVDGKVKLNVGCIQVVYLHKFFMSLLNFSNNFQEAKEALSAATAQAAEKAASSVRDFAQK--SFRLAMDIKLKAPLIIIPQSSTSHNAVEADLGLITVGNRFSLLPVEGRPLPAVIDNMDIQLTQLKLSRISMELDSDQTSSKLLEPVNLVLSVKRNLASSWYQKMAAIEVDGDLKPMKVALSQDDLSVLLRILMENIGEA-----------SDIQSDTALRQENVLVRARTATGSEILEEKLSESKLKETELLESVRFSFNVESLGLTLYNNDPSQPCVHEE---KSCLGEFMLCKMKMSGKMLNNGNMEVSTILTTCT-LDDRRSGIQRVTSRMLGRRDEETSEPMIDVTYSQSVDERSVVAV-LQKLYLCASVEFLLAVADFFVQALPTSPPTQREKTNQLPLK-YVSEP-KIHTEPKAVPAQRT----KLRAVVVDPEVVFVANLMKADAPALV--ASFQCDFSLLSEEAGQTMKANVRSLKVLACPFIRAEGDKAVTTVLRPCSVELETKMPANAPLAGSMTVEEVIVKISPIILNTVITITAAMAPKPKEELSQQKSG-----DQ---NSLWSLMSVANANYWFL---------------GVDTASEVTESFTDVDSSKDGETFG--------------LTVKVVQVTLESGLGHRTVPLLLAESSFSVTAQNWSSLLSVSADMTLEVNYFNETHAVWEPLLERV-DNGKRRWNLKLDM---KNNPVRDKSPVPGDDFIMLPDPRTAITICSKDTMNITVSKCCLNVFNNLAKAFSESTASTFD-HSLKEKSPFTIRNALG--VALIVQHSANLRVPGSSTLGKVHELAVDKSLDLEYSSFERSSRQSALQRQESCLFN---------LSIVPSGYSEISNIPLGKPGRRLYNVRQPMQEESVSVLLQIDASEGNKVITVRSPLQIKNHFS--VPFAILKYSSELGGMVNVGVAEPEKECHVPLEAYRSQLFLLPVGPL----EKLY--------RASTTCIAWKEKVHVSSEVHAVLQCPATESNFLPLVVNTLAMPDDLSHIANHGEVEWDPAYLIHLHPPVTVKNLLPYSIRYLLEGSADIYELQEGSAADVLNSNVSGEIMELILIKYQGRNWTGRMRISRDMPEFITICFNCDTGEALT-VDVCVHVSRVSGKFILSVHSPYWIINKTSRVLQYRA-EDTFVKHPSDFRDVI 2742          

HSP 4 Score: 141.739 bits (356), Expect = 1.693e-32
Identity = 137/548 (25.00%), Postives = 258/548 (47.08%), Query Frame = 3
Query: 2754 ELQVLQDIKNTLVE--KNGAKEIVKLI--VHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSI-MGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTS----VVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKIL 4370
            E+Q   ++K  L E  +  A+E V L   V ++G    ++ FD++V +Y+  + L   +  D   +    +  + KH  G +LL +  +  +   P F T+  NT   L+ +F S++  LH +ALL  +N++NS IP   +   ++ ET    ++    K  +   ++  + + K      S  + +  DR ++A     +R++           G D+          M Q   T    +LR IV       +LD+                            DP    ++KK + I  +E +FS ++ L+   T   GY  D S+VD  V + +G I+I++L+ F++ +  F+++FQ + + ++    + +E AA+ + +  ++  S R  +DI +KAP+I IP++S S NA +VDLG +T  N F++  +       VLE   I L+ L++SR++L  +    +  IL PIN+ + + RNL+    + IP + +   + S+++ + Q D  +   +L  N  E  ++  +V  +K   K   ++
Sbjct:  862 EVQFQFEVKAVLFELTRQAAQEDVVLAFSVSQLGAEGRIRTFDLSVTSYLKKISLDYCEEKDVQNQPLHLITSSDKH--GSDLLKVEYIKADVNGPSFCTLFNNTEQTLKVEFSSLDFLLHTKALLSTINYMNSAIP-QELTANKERETRRRADRAAAGKTVSKGSKDAGVVNFKLSAVLGSFRVAVCDDRSNIA----DIRVQ-----------GIDAS--------VMVQAKETDVFARLRDIV-------VLDV----------------------------DPK--TIHKKAVSIVGEE-VFSFKMTLYPGATEGDGYT-DTSKVDGKVIMRLGCIQIVYLHKFLMSLLMFVDNFQTAKEALSAATAQAAEKAASSVRDFAQK--SFRLAMDIRLKAPLIIIPQSSTSHNAFVVDLGLITVGNRFSLLAAEGFPLPAVLETMDIKLTQLKLSRTVLKHDADHLDIEILEPINLELLVKRNLAAAWYNKIPGVEVNGVLMSMNMSLGQEDLGVLMRILAENIGEG-SKAAAVEGTKASAKDEAVI 1341          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Zebrafish
Match: vps13a (vacuolar protein sorting 13 homolog A [Source:ZFIN;Acc:ZDB-GENE-030926-1])

HSP 1 Score: 494.197 bits (1271), Expect = 1.798e-139
Identity = 365/933 (39.12%), Postives = 545/933 (58.41%), Query Frame = 3
Query: 7368 KNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSS-IVLYCNELVENAGWIKVPPGEA-VPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYE-SHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYIN---EMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKW-NQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVE---EHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSG----SVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNR-TGKTALHSEV-----LNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKK----NVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEI 10094
            K + F +  K  L I++S  SD FS+DTVGS G V CK +   Y VGVKI+ SS  LT++VTFLP+YM++N++  ++  C E  EN  W +  PG+A VPFWP  +  K   +R KVE  +    + F F++  + +L+ L+K   GI V+V +TE + +I    Y +G A   LINH  D+  + + Q +  +   L   Q V Y W +    R   W++     ++   L S+  ED  ++   E K + VSF     R+ LFT++  + K    S  +E   QEI+I L ++G+SLV+N+  QE+A+I ITSS V W  + ++++R K L + ++  LE  + +++         ++   ++ V      VD+    +++P    L R F P + ++Y  S  Q    ++I +IQI NQ+    FP VF P  PP+SV++D+  KPL ++S +   +G +  +R KYF VLIQEM L LD GF+ +IL+  +     +V +  E  +F KD + ++  L   S   T        +++ FHISPIK+HLS SLS     + K    +E+     L+L L+S+G  +TD QDV+FKLG+FE +       Q+   +  HY  QA++QMYVLVLGLDVLGNPFG+IRG+++GVE  FYEP +GA+ GPEEF+EG+A GVK+L G AVGG AGA SRI G M KG+AA+T+D +YQ+KRRE + K+P  L  GL +GGKGL+ GFV G+TG+VTKPI GA+K+G  GFFKG+GKGLVG +TRP  G++D ASS+F+GI+R A+ + +V  +RPPRFI +DG+IR Y  RE  G+ +          I N  +  + Y    K    + + +T + IF + +    G  + +WQ  +++    P I E +  +    KE+ + +F      K I
Sbjct: 2290 KPRNFLQNNKIRLMISESELSDDFSIDTVGSYGDVKCKGRQKDYLVGVKIDASSFSLTRLVTFLPFYMLVNRTKHVIRVCEEGQEN--WTEARPGQAAVPFWPERDSKK---LRVKVEGSQS-SPRAFDFHQPENCLLLHLDKSLGGIIVDVNLTEHSAVILFSDYHDGAAPFLLINHTKDET-LQYHQSSQAEIDELSPGQAVYYTWAEPTGSRILSWKL----GKYKGELKSE--EDQLLDVDKEGKLFLVSFYEGLQRVALFTSEQRIFKKICDSEKIELAQQEISILLQNMGISLVNNNSSQEVAFIGITSSDVVWETKPKKRSRWKVLSVKEAAVLEQHYRDYMENGPVDSTIIDLDNDYQVLFAANGVDMR---IMQPYDAPLRRNFLPALKVEYSVSARQKTYRVQINRIQIQNQLQGAIFPFVFYPIKPPKSVSMDSEPKPLADVSIITRAAGHSDILRIKYFMVLIQEMDLRLDLGFLYAILELFTAEHTSAVTSEQEVELFDKDIEYLKTELNHVSATDT----SPISLYEYFHISPIKLHLSLSLSSGGEDSNKQERETELIPVQSLHLLLKSIGATLTDAQDVLFKLGFFELRYQFRTTQQLQWEVVRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFMEGMALGVKALVGGAVGGLAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLREGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGMGKGLVGALTRPTGGIIDMASSTFQGIKRAAELSQDVESLRPPRFIHEDGVIRPYREREGLGSQM-------LQSIENGRFAKYRYFAHAKVNESDFLMITKRGIFFVTKG-TFGQLTCEWQYLFDEFTKAPMIVEGRR-LRIEAKERVKSVFHAKEFGKII 3193          

HSP 2 Score: 259.225 bits (661), Expect = 4.467e-68
Identity = 239/936 (25.53%), Postives = 438/936 (46.79%), Query Frame = 3
Query: 3441 ITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTS-----VVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESI-----------TVQFGFKMEFLDVELFKGDANPN-EWASEMPPDKCLTKMKCENLSVEGSMHL--NSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSD--FISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADF------EIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLT--AQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQY-----SFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGE 6146
            I A L  L+V +  EK   I +ISI G+ ++V+    + E+   + ++ I D    A+YKK + I  D+ +F  ++      T    Y  D+  VD  V + VG I++IFL  F+  I  F+N+FQE+   +A    + +E AA  +  + E   S+R  LD+++KAPV+++P++S S N ++ DLG +T KN F I  S +      V++   + LS+L++ R+   ++   +E  +L P+N+ + + RNLS +  H+IP I I A +  +S+ +SQ D  +    L  N +E  +  P  P S +  K   +  K  +     T    ++           T++  FK + + + L+    +PN E       D+ L   +    ++  S+H+  +S+M+ S++L+N  +LD +    K I   +  ++ D  D   + + + Q ++  ++L   +  +Y+C  +++L+ + D F+KA                G+   P K  K+P ++  +P           E++V V  PEI+ V D+   ++ A+++ T   L          +   ISD + V CPF  E R+ N   ++ P  +     +         L++  L + ++P +I ++  I   L+  A++  E  +            I  + WE+R   + +  F+ +   E E+++T          V +++              G++L         + +  I + LE+ V  + VPML+ + S  GD+K+  T I L   +S+ V+Y+N+ + +WEPLLE   +E    +     + +++      SG+ DE D     ++      I   S+D L +T+SK  L +LSNLG AF +A     E  ++    P+VVKN  G  V    ++ F  +P   + + +V    DGE
Sbjct: 1083 IRADLNCLKVFIRGEK-ARISEISIEGLVSEVQMKKKSVEILANLKNIIILDCDKEALYKKAVSIA-DKEVFDFRMVNHVDATEGDAYT-DMHIVDTSVTLTVGCIQVIFLNKFMSSILAFINNFQEAKNALAEVTAQAAEKAATGVLELAER--STRISLDVDIKAPVVFLPQSSVSHNVIVADLGLVTVKNRFEIVSSPTHTKIPPVMDIMAVGLSDLKMYRTTFTDDKFNSEIQLLKPVNMDLSIKRNLSASWYHSIPDIQINAHLKPMSLILSQEDLTVVLKTLSENLSET-SDAPPAPSSSD--KGDTVSNKGSQGSGGNTVVTAAVVEGQKSGKLKNTLKLNFKFDSMTLVLY----SPNSEIQLSDSRDESLKLAEFTLGTISTSVHMFSDSSMKASLKLTNC-LLDDKRQGVKMITSRMMAMRQDREDSVMVDVSYRQSRD-GTRLDTLVQDLYLCASMEFLLTVADVFLKALSD-------------GF-SGPPKSNKQPASTSKDPAAPAPPVVAKTEMNVVVKNPEIVFVADLTRAEAPALVMTTQCELVMKSEPSGQCMTAAISDLKIVACPFLREKRENNVTTVLQPCEVFFQSRQSNTEPQCIELSISALTLKVSPIIINTVITIQTALSPAAETPQELESP-----------IPQDLWERRSLRDLKLWFLEE---EGEEVST---------SVTRLI------------PQGESLK--------MSIASICLTLEAGVGHRTVPMLLAKASFQGDVKDWSTLINLHCHLSMEVNYYNEMMGLWEPLLEPLDDEDKDGFRPWTLALKMKKKPVVSSGVSDEVDG----AVPDYKTVISFTSKDQLNVTLSKCGLHMLSNLGTAFAEAAKQTAECFQTD-QTPFVVKNRLGLAVSVLHSEVF--RPIGKESKDRVVELPDGE 1940          

HSP 3 Score: 58.5362 bits (140), Expect = 2.266e-7
Identity = 40/130 (30.77%), Postives = 66/130 (50.77%), Query Frame = 3
Query: 6795 LHNQLPIKISYQSQIF----SHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFK--AKEGYSESF--MDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEH 7160
            L N LP  I+++ +      S  +K G    + TA +           Y +++W     L  + +E+S I F+  A+E  +ES   +D+ +H+  + GQ  + ++SPYWM+NKT   L YKA DD   +H
Sbjct: 2149 LRNLLPYPITFKLKGIGDSPSVSLKEGHSSQIHTAIMDFSVLDLRLLGYLQQDWESQYSLSSEQDEISFIKFRSLAREEETESRPELDIAMHVKKEEGQVVLAVHSPYWMVNKTSRLLQYKA-DDIHRKH 2277          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Zebrafish
Match: vps13d (vacuolar protein sorting 13 homolog D [Source:ZFIN;Acc:ZDB-GENE-050912-1])

HSP 1 Score: 187.963 bits (476), Expect = 1.285e-46
Identity = 119/413 (28.81%), Postives = 212/413 (51.33%), Query Frame = 3
Query:   27 VFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPET-TNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNR-CRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKK 1259
            + E LV  ++N YLG YV  L+  QL + LL G   LENL ++++A   FDLP +V+ G I K+TL+IP+   +S+P +  +  L ++I P    +YD  +ER    E K K+L +  E+K K+ +    + T       +  +IV+N+++ I  +H+R+ED  + P + +A G+ + N+S Q C       +I++       K +++   +VYW++          + ++  M   + S E +     ++ +P+  S ++  +   E   +   PR+   ++LE +    ++ QY  I  FL  +DR   +  FRK+RP + +  N   WW F  N  L E +R++RR  SW +   HR R  +LYT ++ Q+L    LS  E+  ++  E+E  L  + + R+   +  +K
Sbjct:    1 MLEGLVAWVLNTYLGKYVSNLNTDQLSIALLKGAVELENLPLRKDALRGFDLPFEVKAGVIGKITLQIPFYRPHSDPWVICMSQLNLIIGPALPQEYDEVQEREAEREQK-KQLLKALEDKWKKGESYWYSVTA-----SVVTRIVENIELKIQGVHLRFEDDFSNPEKPYAFGVCIKNVSAQNCSKEPAQKLIRQ-------KELEISEFSVYWDTECTMLGDLPSSEIQERMSKCMQSREHQ-----YIFEPVCASVLVRRNASKEPLRSRNTPRIEGQVQLEPLSLRLSQVQYQQIMAFLKELDRREKEMLFRKWRPKLTISGNCRQWWMFAINANLHE-IRKQRRHGSWDFA-LHRARDAKLYTSLYVQRLKGVTLSPQEESEMERIEDEQTLEELQILRETVYVSFRK 393          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Xenopus
Match: git2 (G protein-coupled receptor kinase interacting ArfGAP 2 [Source:Xenbase;Acc:XB-GENE-949238])

HSP 1 Score: 554.288 bits (1427), Expect = 1.837e-157
Identity = 367/924 (39.72%), Postives = 555/924 (60.06%), Query Frame = 3
Query: 7305 AEDLH-DHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG-----WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPF-YESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINE-MKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILD-FVSGSVPTYDEE--AMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLS----RVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTI 10031
            AED+H  H        + +   KN  F+K  K  L I+ S WS  FSLDTVGS G V C    + Y VGV I +SS  L++IVT  P+Y ++N+S + +   E+  +       W  V   E +PFWP +  GK  +     E     +++PF F  + +  L+ LE    GI V+V I + + +I    Y +G A   +INH+    ++ + Q  T  E  L   +  ++AW+D    ++  W+     ++   +L  D    F  N+ ++ +WVSF+    R+LLFT D+ V   AR + ++E  DQE+T+SLHS+GLSLV+N  + E++YI I SSGV W +++RK++ +P    Q   LE A+++++   +     VEE+ V V   +V +  +L   P KC + R F  G++++++ SPHQ  +  ++Y +Q+DN +    FPVVF P  PP+SVALD+  KP I++S +   +  +  M++KYF VL Q M L LDQGF+ +++  F   + P  +++   +  KD   +   L+E+S             F++FHISP+K+HLS SL     + N+  K  +    +NL L+S+G  +TD+ D+IFKL  FE +      NQ+ + +  HY  Q L+Q+YVLVLGLDVLGNPFG+IRG+ +GVE  FYEP +GAV GPEEF EG+  G+KSL GH VGGAAG VSRI GT+GKG+AA+T+D +YQ+KRR+++ ++P++ G  LA+GGKG + G V GVTG++TKP++GAKK+G  GFFKG+GKGLVGVV RP  G+VD ASS+F+GI+RVA++  EV  +RPPR I +DGIIR Y   EA+G  ++  +  K   +  E+Y +    +++K  + +TN+R+  +   EILG   I W   +E+   PP ++ +  T+
Sbjct: 2677 AEDIHVKHPSDYRDVILFSFKKKN-IFSKN-KIQLCISTSSWSTGFSLDTVGSYGCVKCPAVKMEYLVGVNIKMSSFNLSRIVTLTPFYTLVNKSKLEIEVGEMATDVSPTDIKWNYVSSSECIPFWPENISGKLCVRVIGCEG----ISKPFFFNKQDNGTLLSLESMNTGIIVDVSIADHSTVITFSDYYKGAAPALIINHM-PHAKLTYNQSGTEIEKELGPGEARLFAWEDPTGTKKLNWKCASTTRE--VDLLKDESGQFAYNQNVQIHWVSFLDGRQRVLLFTEDVAVVTKARQAEEMEQSDQEVTVSLHSLGLSLVNNDSRHEISYIGIISSGVIW-EMKRKHKWQPFNQKQIFQLEGAYEKYLTNEEQGWVSVEENFV-VCFSKVPMEMRL---PIKCSIRRNFMSGIYMEFKQSPHQRSLRAQLYWLQVDNHLPGSMFPVVFHPVIPPKSVALDSEPKPFIDVSIITRFNEFSKVMQFKYFMVLTQCMALKLDQGFLAAVIGLFTPETDPNAEKQRTQLIQKDLDSLNTELMESSLTDISFPS----FFEHFHISPLKLHLSLSLGAGGEQSNKEEKEIIAIASVNLLLKSIGATLTDVDDLIFKLACFELKYQFYKQNQLRNLVVRHYTEQFLKQVYVLVLGLDVLGNPFGLIRGLTEGVEAFFYEPFQGAVQGPEEFAEGLVIGMKSLLGHTVGGAAGMVSRITGTVGKGLAAITMDKEYQQKRRQEMGRQPKDFGESLAKGGKGFLRGVVGGVTGIITKPVEGAKKEGAAGFFKGIGKGLVGVVARPTGGIVDMASSTFQGIQRVAESTEEVSPLRPPRLIHEDGIIRPYNESEAQGFKLFQNLNVK--KLDGEMYRYSCCLQNRKANLMVTNRRLICMKEVEILGHRMIDWTYTFEEFVHPPIVDGNTLTL 3580          

HSP 2 Score: 523.857 bits (1348), Expect = 3.538e-148
Identity = 405/1398 (28.97%), Postives = 687/1398 (49.14%), Query Frame = 3
Query:  189 KVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRND---PKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLAS--DGSDEL--QKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIXXXXXXXXXFKDALE---IMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQ---DIKNTLVEKNGAKE-----IVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPS--TKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEA 4310
            K+   +I KLTLKIPWK+LY + V+A L+GL++L++P + +KYD +KE   + ETKQ+EL RIEE   K  D   PK     KD+F EKL  Q++KNLQI I++IHIRYED   C +            S QTC+ S    I+ E +T+  +K V++DS + YWN   K +  + R  +   +K  I S  RK  D  ++ +P++ SA ++++   E      P++   ++++ +     + QY      L+S+D       +RKYRP + ++ +   WW +  N ILE  ++RR  MWSW  I  HR   ++Y + ++ KL   KL +     ++ CE+ LD+ NI L RQQA+ +  +  +KL  K SS G      G   +  S   A+  D  DE+   +K+    T EEKAKL+ AIGYS+ + +  +P  YV+  LN +L +    I ++ +   + +++ I  +S  V QR  A A+ +   + +  VTG       VP+LV T   ++  +LL + FETNP    A+Q + + ++P+EIIYD+ T+N + +F    + + L ++++  L  +EE+KE TATG+          ++ ++I                   +L+ + S+    E T+                L+ + + E AYDKF + +  +Q L   +G+DW+K R + SS  HIL+P   E  L+K ++  D+ +P+  + G LP L + ISD+ +Q V +L+  IP PQ       PT                      +N+ + AI +LP      L   I++    +++  D+ E    +D+N     K      +I    ++    V N  EL  L    ++K   +E    +E     I+   V ++GT   +K + +   +Y+  + L    + D     K P+ L  +      + LL +  +  +   P F T   NT   L+  F ++++ LH EALL  ++F+   IP L        E   +P ++                                       +K ++  KA  +S++ D   +    K+F            A+L    V V +EK N+I +I I GM          TEV   + D+ + D +P  ++KK + I  +E +FS ++ L+ + T    Y+ D+S+VD  +++ VG I+I+FL+ F++ +  FLN+FQ + + V+    + ++ AA  + ++ E   S R  +D+++KAP+I +P++S S NA++ DLG L   N F +    DQ     ++   I L+  ++SR++L + + + +  ILHPINI + + RNL+    H IP + +   + +++VG+SQ D ++   VL  N  EA
Sbjct:   10 KMNTFYIYKLTLKIPWKNLYGDAVVATLEGLFLLVVPGASIKYDAQKEEKSIQETKQRELLRIEEALQKAADKDIPKDAK--KDTFMEKLLTQVIKNLQIKITDIHIRYEDD--CHDICTVYSHIFHLYSTQTCNESWKPCILNE-ATKIIYKLVRLDSFSTYWNVKSKLYYRKPREDILTALKEGILSEARKPKDYQYIFKPVNASAKLYINPLAEM-ELKTPKIDWIMEVQNVAIEVTKPQYLSFMDLLESVDYMVRNAPYRKYRPQLSLQEDTKQWWLYAINSILEVHIKRRTHMWSWNNIAKHRQNLKMYKNAYKTKLTQAKLPEETVKQLQACEKLLDVFNIVLARQQAQAEIIRSGQKLMAKKSSAGGEKKGGGWFGSFWSKKEATKKDDVDEIFASEKIDDFMTPEEKAKLFTAIGYSDSSHNLALPKAYVAHILNFKLLRTSITIRED-IHTPETLQIQIIDLSTKVFQRPGAQAVKIEAKLEHWYVTGL-SQQNIVPSLV-TSIGNRESSLLRIVFETNPQHIKADQVLILESQPVEIIYDAVTINALAEFFQTQKGMDLEQLTSATLMKLEEIKEKTATGI----------NLVLEI----------------IDFILLQIISVDQAEETTN----------------LSLEDLMEKAYDKFDVRIKNVQILFGTKGEDWKKAREKGSSSLHILQPLDIELKLEKAMVERDSRMPRFKILGELPLLHIKISDKKIQGVFQLVNSIPLPQKT---STPT---------------------NANKKIPAIAILPRRFEKLLKKEIEQSLFLEDDQSDSNEEFFDVDDNFPAIVKTKQIHKQISVNAEMSNTEVTNVEELTDLNLKFEVKEVKIEFTKQQEKLQETILVFNVLQLGTESCIKTYSLTSVSYLKKISL---DYYDTKGFRKQPLHLISSSDKNASE-LLKVEYVKADTKGPQFQTTFDNTEQTLKVFFSAMDLLLHSEALLSTISFLTESIPNL-------PENVRAPKQQ---------------------------------------DKESLVKKAAKISKEKDIFAF----KLF------------ANLDAFNVNVWDEK-NHIAEIKIQGMACHFFIQSNQTEVFARLKDIIVNDVNPQTIHKKAVSI-MEEEVFSFRLMLYPEATQGDAYK-DVSKVDGKLDLRVGCIQIVFLHKFLMTLLKFLNNFQAAKERVSMATAQAAQRAATSVKDLAER--SFRLLMDVHLKAPLIVVPQSSVSCNAIVADLGLLKINNRFTLVSSEDQQLPPFVDHMTINLTEFKLSRTMLQDGSFQPDVEILHPININLSINRNLAATWYHKIPVLEVKGHLNTMNVGLSQEDVSVLLNVLVENLGEA 1261          

HSP 3 Score: 337.035 bits (863), Expect = 1.684e-91
Identity = 367/1510 (24.30%), Postives = 663/1510 (43.91%), Query Frame = 3
Query: 2829 VHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFA----IDQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNE------AINQVPSVPESKEEVKSRKILPKD----DKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQD----KNSNSKLIVDI-AGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVE---PRLADFEIDVKVARPEILLVEDMLNPDSNAIMV--QTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESK--IYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYS--FEIEYISCDDSGLFDEEDFEDLISIEP-SAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFY--INKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRG--------NVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKC---YNVPIPT-----LLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSI--EEVQIVNSS---ETLRTYKLDINVPLALHNQLPIKISY--QSQIFSHDIKPG-CLELVRTACLKVQSNIKVTY--HYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESF-MDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAV 7184
            V ++G    +    M    Y+  + +A   F+ PS   P+ L  +        LL +  +  ++  P+F T+H NT         S++  LH +ALL ++NF++S +P  NI+EE +        KE   K++ L+                SI+                     A  E  ++N  DS               ++A L  L + V +E   NI DI I GM A V      TEV   + D  I D     ++KK ++I  DE +F+ +++++ + T    Y  D+S VD  + + VG I II+L+ F++ +  FL+ FQ + + ++    + +E AA+ +    ++  S R  +DI++KAPVI IP++S S NA++ DLG +  +N F+    +D     V+++  I L+ L++SR++L+  + K E  IL P+N+ + + RNL+ +    IP + +   +  + + +SQ D ++   VL  N  E       +    S+ + K+EV+ +    KD    + S     A   S+T+ + FK E L + L++ D +             L +++   L+  G+M+ + +M+ SV L    + D +    K  + +I     D  D +  + + D    + +   LI  I   +Y+C  +++LM + DFF++A P      K     VR      R  + KP A   E   P      + V +  PEI+ V +++  D+ A+ V  Q NF L    +  S  +   + +F+ + CPF  E R  N   ++ P S+ +             L++E +II ++P ++ ++  I++ L  +  +E          N  +L+  N W+ R  D     F+                   G D+AQ + +  Q              EHKE +    L +  +++ LE  +  + VP+L+ E +    +KN  + I +    ++ V Y+N++  VWEPL+E      +R++S  FE++     D  LF  +DF  ++  EP SAI+I   S+D + +T+SK  L V  NL KAF +  +   + + S   AP  +KN  G  ++     N   ++   +S              Q +D VDE QSL  E    +++K G   ++ R+  S+    +    ++   N   ++         N  +    +++V   A  G K + +RS LQ+ N+     T+Y     +          LLE +   +    ++VP+ +      L   G +    K+     S S   W    +        L C ++        V  S   +E+  ++ +   E    Y + +   L + N +P  I +  +    +H++  G  L+++++   ++   I   +   YQ + W G + +   + E   + F A    SE   +DL VH+     +  +++YSPYW++NKT   L YKA +D  V+H +   D +
Sbjct: 1338 VSQVGAEARVGSQLMTASVYLKKISMACNDFMGPSG-GPLLLINSSDDDNAP-LLKMEYVKADQNGPNFKTIHDNTEQSFNISVSSLDFILHTQALLSLMNFLSSAVPSHNILEENRP-------KESKAKESRLK----------------SII---------------------ARPESYNFNTEDS-----------FSLKVSAKLNALNIFVCDEI-CNIADIRIQGMDASVAVQTKQTEVCARLEDFVIFDVDSKTIHKKAVFIMGDE-VFNFKMSMYPEATEGISYS-DMSAVDGKICLRVGCIHIIYLHRFLMSLLNFLDKFQAAKEALSTATSQAAEKAASSMKEFAQK--SFRLSMDIDLKAPVIIIPQSSVSANAIVADLGFIRIQNKFSLVPVVDCPLPPVVDQMNIQLTELQLSRTVLMSGSDKPELEILQPVNLLVSVQRNLASSWYSEIPALQLVGDLKHMQIALSQEDLSILMSVLFENLAEKPSLPRVVETTKSIEQVKKEVECQSYQQKDTMKTEDSVEQDVAEALSVTLNYKFKFESLSIVLYQSDCSKETALWLHNESLRLGELRLHLLTSSGNMYSDGSMDASVYLKTCTLDDLREGITKATSRMI-----DQKDGVCERNMVDITYKQGAQDCLITTILEKLYVCASMEFLMTVADFFIQAMPQSPSGEKSMQKHVR------RSTQTKPRAESGESHPPSQKKVNVKVLIMDPEIVFVANLMKADAPALSVSFQGNFTLDS--LSGSQKVAAIVKEFKVLACPFLRELRGSNITTVMQPCSLQIEGTIDAAGAKNLILSLEEVIIKVSPIILNTVQTIIDALKPKPKEEEVNE------NLTELV--NIWDVRSIDSCSNWFL-------------------GVDMAQEITETFQS------------SEHKEKQESFELEVKSVQITLECGLGHRTVPLLLMESTFSTKVKNWTSFINITADTTLEVHYYNENYAVWEPLIERV-NCGSRRWSLKFEMKNNPVMDKSLFPGDDF--ILIPEPRSAINI--YSKDTMNITISKCTLTVFGNLAKAFSEGTASTYD-YDSKDRAPLTIKNALGIPIIVQHGSNMRRNIPSPHS--------------QTHD-VDEGQSLELEFCAVELLKRGQHSALHRQDSSLLTVGIVPHGYTEMANIPVTKTARRLYNVRNPQQENFVSVLVQIDATEGNKVITIRSPLQIKNHFSIPFTVYKFVASAK---------LLEPIGVARQDEEFHVPLDSYRCQLFLRPAGALEEQFKE-----STSYITWKEMVHRSSEVRCTLQCPASEISFLPLMVNCSAVPDEINFISRNGEKEWDPAYIIHLYPTLTVRNLVPYSIRFLLEGAAETHELAEGSSLDVLQS---RISGEIMELFLIKYQGRNWNGHIRVHENLSEFFPVLFTADS--SELLTVDLSVHVRHIGNRMVLSVYSPYWIINKTSRILQYKA-EDIHVKHPSDYRDVI 2692          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Xenopus
Match: git2 (G protein-coupled receptor kinase interacting ArfGAP 2 [Source:Xenbase;Acc:XB-GENE-949238])

HSP 1 Score: 548.51 bits (1412), Expect = 6.979e-156
Identity = 365/930 (39.25%), Postives = 551/930 (59.25%), Query Frame = 3
Query: 7305 AEDLH-DHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG-----WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPF-YESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINE-MKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILD-FVSGSVPTYDEE--AMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLS----RVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCK------SKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTI 10031
            AED+H  H        + +   KN  F+K  K  L I+ S WS  FSLDTVGS G V C    + Y VGV I +SS  L++IVT  P+Y ++N+S + +   E+  +       W  V   E +PFWP +  GK  +     E     +++PF F  + +  L+ LE    GI V+V I + + +I    Y +G A   +INH+    ++ + Q  T  E  L   +  ++AW+D    ++  W+     ++   +L  D    F  N+ ++ +WVSF+    R+LLFT D+ V   AR + ++E  DQE+T+SLHS+GLSLV+N  + E++YI I SSGV W +++RK++ +P    Q   LE A+++++   +     VEE+ V    + ++     M  P KC + R F  G++++++ SPHQ  +  ++Y +Q+DN +    FPVVF P  PP+SVALD+  KP I++S +   +  +  M++KYF VL Q M L LDQGF+ +++  F   + P  +++   +  KD   +   L+E+S             F++FHISP+K+HLS SL     + N+  K  +    +NL L+S+G  +TD+ D+IFKL  FE +      NQ+ + +  HY  Q L+Q+YVLVLGLDVLGNPFG+IRG+ +GVE  FYEP +GAV GPEEF EG+  G+KSL GH VGGAAG VSRI GT+GKG+AA+T+D +YQ+KRR+++ ++P++ G  LA+GGKG + G V GVTG++TKP++GAKK+G  GFFKG+GKGLVGVV RP  G+VD ASS+F+GI+RVA++  EV  +RPPR I +DGIIR Y   EA+G  ++            ++ V   YC+      ++K  + +TN+R+  +   EILG   I W   +E+   PP ++ +  T+
Sbjct: 2290 AEDIHVKHPSDYRDVILFSFKKKN-IFSKN-KIQLCISTSSWSTGFSLDTVGSYGCVKCPAVKMEYLVGVNIKMSSFNLSRIVTLTPFYTLVNKSKLEIEVGEMATDVSPTDIKWNYVSSSECIPFWPENISGKLCVRVIGCEG----ISKPFFFNKQDNGTLLSLESMNTGIIVDVSIADHSTVITFSDYYKGAAPALIINHM-PHAKLTYNQSGTEIEKELGPGEARLFAWEDPTGTKKLNWKCASTTRE--VDLLKDESGQFAYNQNVQIHWVSFLDGRQRVLLFTEDVAVVTKARQAEEMEQSDQEVTVSLHSLGLSLVNNDSRHEISYIGIISSGVIW-EMKRKHKWQPFNQKQIFQLEGAYEKYLTNEEQGWVSVEENFVCFSKVPME-----MRLPIKCSIRRNFMSGIYMEFKQSPHQRSLRAQLYWLQVDNHLPGSMFPVVFHPVIPPKSVALDSEPKPFIDVSIITRFNEFSKVMQFKYFMVLTQCMALKLDQGFLAAVIGLFTPETDPNAEKQRTQLIQKDLDSLNTELMESSLTDISFPS----FFEHFHISPLKLHLSLSLGAGGEQSNKEEKEIIAIASVNLLLKSIGATLTDVDDLIFKLACFELKYQFYKQNQLRNLVVRHYTEQFLKQVYVLVLGLDVLGNPFGLIRGLTEGVEAFFYEPFQGAVQGPEEFAEGLVIGMKSLLGHTVGGAAGMVSRITGTVGKGLAAITMDKEYQQKRRQEMGRQPKDFGESLAKGGKGFLRGVVGGVTGIITKPVEGAKKEGAAGFFKGIGKGLVGVVARPTGGIVDMASSTFQGIQRVAESTEEVSPLRPPRLIHEDGIIRPYNESEAQGFKLF------------QVSVHRTYCRLYCCLQNRKANLMVTNRRLICMKEVEILGHRMIDWTYTFEEFVHPPIVDGNTLTL 3188          

HSP 2 Score: 358.607 bits (919), Expect = 4.812e-98
Identity = 262/819 (31.99%), Postives = 423/819 (51.65%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRND---PKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLAS--DGSDEL--QKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQ-TAPP 2456
            ++ ES+V +L+N++LGDYV  LD+SQLKLG+ GG   +   D+      + ++P KV+ G I KLTLKIPWK+LY + V+A L+GL++L++P + +KYD +KE   + ETKQ+EL RIEE   K  D   PK     KD+F EKL  Q++KNLQI I++IHIRYED   C +            S QTC+ S    I+ E +T+  +K V + +L+V                   ++ N I              +P++ SA ++++   E      P++   ++++ +     + QY      L+S+D       +RKYRP + ++ +   WW +  N ILE  ++RR  MWSW  I  HR   ++Y + ++ KL   KL +     ++ CE+ LD+ NI L RQQA+ +  +  +KL  K SS G      G   +  S   A+  D  DE+   +K+    T EEKAKL+ AIGYS+ + +  +P  YV+  LN +L +    I ++ +   + +++ I  +S  V QR  A A+ +   + +  VTG       VP+LV T   ++  +LL + FETNP    A+Q + + ++P+EIIYD+     + +F    + + L ++++  L  +EE+KE TATG+ +L     + D         FIL          +++ VD       +E T+                L+ + + E AYDKF + +  +Q L +         + + SS  HIL+P   E  L+K ++  D+ +P+  + G LP L + ISD+ +Q V +L+  IP PQ T+ P
Sbjct:    1 IMLESVVADLLNRFLGDYVENLDRSQLKLGIWGGK-IISLFDVL--TLFELNVPFKVKAGQIDKLTLKIPWKNLYGDAVVATLEGLFLLVVPGASIKYDAQKEEKSIQETKQRELLRIEEALQKAADKDIPKD--AKKDTFMEKLLTQVIKNLQIKITDIHIRYED--DCHDICTVYSHIFHLYSTQTCNESWKPCILNE-ATKIIYK-VSIYNLSVI-----------------NMLHNCI-------------FKPVNASAKLYINPLAE-MELKTPKIDWIMEVQNVAIEVTKPQYLSFMDLLESVDYMVRNAPYRKYRPQLSLQEDTKQWWLYAINSILEVHIKRRTHMWSWNNIAKHRQNLKMYKNAYKTKLTQAKLPEETVKQLQACEKLLDVFNIVLARQQAQAEIIRSGQKLMAKKSSAGGEKKGGGWFGSFWSKKEATKKDDVDEIFASEKIDDFMTPEEKAKLFTAIGYSDSSHNLALPKAYVAHILNFKLLRTSITIRED-IHTPETLQIQIIDLSTKVFQRPGAQAVKIEAKLEHWYVTGL-SQQNIVPSLV-TSIGNRESSLLRIVFETNPQHIKADQVLILESQPVEIIYDA-----VTEFFQTQKGMDLEQLTSATLMKLEEIKEKTATGI-NLVLEMPFID---------FIL---------LQIISVDQ------AEETT---------------NLSLEDLMEKAYDKFDVRIKNVQILSV--------CKEKGSSSLHILQPLDIELKLEKAMVERDSRMPRFKILGELPLLHIKISDKKIQGVFQLVNSIPLPQKTSTP 723          

HSP 3 Score: 268.085 bits (684), Expect = 1.163e-70
Identity = 300/1258 (23.85%), Postives = 548/1258 (43.56%), Query Frame = 3
Query: 3522 MRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFA----IDQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDI-AGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMV--QTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESK--IYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLE-VYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEP-SAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFY--INKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRG--------NVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPT-----LLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSI--EEVQIVNSS---ETLRTYKLDINVPLALHNQLPIKISY--QSQIFSHDIKPG-CLELVRTACLKVQSNIKVTY--HYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESF-MDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAV 7184
            M A V      TEV   + D  I D     ++KK ++I  DE +F+ +++++ + T    Y  D+S VD  + + VG I II+L+ F++ +  FL+ FQ + + ++    + +E AA+ +    ++  S R  +DI++KAPVI IP++S S NA++ DLG +  +N F+    +D     V+++  I L+ L++SR++L+  + K E  IL P+N+ + + RNL+ +    IP + +   +  + + +SQ D ++   VL  N  E     PS+P   E  KS        +  +A    +ES+                                  L +++   L+  G+M+ + +M+ SV L    + D +    + I +      N   +   +     + +   LI  I   +Y+C  +++LM + DFF+           +AM      EKN      K  + PVE ++    + V +  PEI+ V +++  D+ A+ V  Q NF L    +  S  +   + +F+ + CPF  E R  N   ++ P S+ +             L++E +II ++P ++ ++  I++ L  +  +E          N  +L+  N W+ R  D     F+                   G D+AQ + +  Q              EHKE +    L +  +++ LE  +  + VP+L+ E +    +KN  + I +    ++ V Y+N++  VWEPL+E V          FE++     D  LF  +DF  ++  EP SAI+I   S+D + +T+SK  L V  NL KAF +  +   + + S   AP  +KN  G  ++     N   ++   +S              Q +D VDE QSL  E    +++K G   ++ R+  S+    +    ++   N   ++         N  +    +++V   A  G K + +RS LQ+ N+     T+Y     +   E        EE      ++VP+ +      L   G +    K+     S S   W    +        L C ++        V  S   +E+  ++ +   E    Y + +   L + N +P  I +  +    +H++  G  L+++++   ++   I   +   YQ + W G + +   + E   + F A    SE   +DL VH+     +  +++YSPYW++NKT   L YKA +D  V+H +   D +
Sbjct: 1192 MDASVAVQTKQTEVCARLEDFVIFDVDSKTIHKKAVFIMGDE-VFNFKMSMYPEATEGISYS-DMSAVDGKICLRVGCIHIIYLHRFLMSLLNFLDKFQAAKEALSTATSQAAEKAASSMKEFAQK--SFRLSMDIDLKAPVIIIPQSSVSANAIVADLGFIRIQNKFSLVPVVDCPLPPVVDQMNIQLTELQLSRTVLMSGSDKPELEILQPVNLLVSVQRNLASSWYSEIPALQLVGDLKHMQIALSQEDLSILMSVLFENLAEK----PSLPRVVETTKSI-------EQETALWLHNESLR---------------------------------LGELRLHLLTSSGNMYSDGSMDASVYLKTCTLDDLR----EGITKATSRYANGVCERNMVDITYKQGAQDCLITTILEKLYVCASMEFLMTVADFFI-----------QAMPQSPSGEKNGLFIWYKYQSRPVEEKV---NVKVLIMDPEIVFVANLMKADAPALSVSFQGNFTLDS--LSGSQKVAAIVKEFKVLACPFLRELRGSNITTVMQPCSLQIEGTIDAAGAKNLILSLEEVIIKVSPIILNTVQTIIDALKPKPKEEEVNE------NLTELV--NIWDVRSIDSCSNWFL-------------------GVDMAQEITETFQS------------SEHKEKQESFELEVKSVQITLECGLGHRTVPLLLMESTFSTKVKNWTSFINITADTTLEVHYYNENYAVWEPLIERVNCGSRRWSLKFEMKNNPVMDKSLFPGDDF--ILIPEPRSAINI--YSKDTMNITISKCTLTVFGNLAKAFSEGTASTYD-YDSKDRAPLTIKNALGIPIIVQHGSNMRRNIPSPHS--------------QTHD-VDEGQSLELEFCAVELLKRGQHSALHRQDSSLLTVGIVPHGYTEMANIPVTKTARRLYNVRNPQQENFVSVLVQIDATEGNKVITIRSPLQIKNHFSIPFTVYKFVASAKLLEPIGVARQDEE------FHVPLDSYRCQLFLRPAGALEEQFKE-----STSYITWKEMVHRSSEVRCTLQCPASEISFLPLMVNCSAVPDEINFISRNGEKEWDPAYIIHLYPTLTVRNLVPYSIRFLLEGAAETHELAEGSSLDVLQS---RISGEIMELFLIKYQGRNWNGHIRVHENLSEFFPVLFTADS--SELLTVDLSVHVRHIGNRMVLSVYSPYWIINKTSRILQYKA-EDIHVKHPSDYRDVI 2305          

HSP 4 Score: 135.576 bits (340), Expect = 1.436e-30
Identity = 83/274 (30.29%), Postives = 154/274 (56.20%), Query Frame = 3
Query: 3519 GMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPS 4328
            GM          TEV   + D+ + D +P  ++KK + I  +E +FS ++ L+ + T    Y+ D+S+VD  +++ VG I+I+FL+ F++ +  FLN+FQ + + V+    + ++ AA  + ++ E   S R  +D+++KAP+I +P++S S NA++ DLG L   N F +    DQ     ++   I L+  ++SR++L + + + +  ILHPINI + + RNL+    H IP + +   + ++++ +S  D+ +    L S  N   + VPS
Sbjct:  870 GMACHFFIQSNQTEVFARLKDIIVNDVNPQTIHKKAVSI-MEEEVFSFRLMLYPEATQGDAYK-DVSKVDGKLDLRVGCIQIVFLHKFLMTLLKFLNNFQAAKERVSMATAQAAQRAATSVKDLAER--SFRLLMDVHLKAPLIVVPQSSVSCNAIVADLGLLKINNRFTLVSSEDQQLPPFVDHMTINLTEFKLSRTMLQDGSFQPDVEILHPININLSINRNLAATWYHKIPVLEVKGHLNTMNISVSSLDFILHTQALLSLMNFLSSAVPS 1139          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Xenopus
Match: VPS13A (vacuolar protein sorting 13 homolog A [Source:NCBI gene;Acc:100488998])

HSP 1 Score: 545.814 bits (1405), Expect = 2.995e-155
Identity = 376/926 (40.60%), Postives = 534/926 (57.67%), Query Frame = 3
Query: 7368 KNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEG-----LEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVS--GSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTG------KTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITE----KCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEI 10094
            K + F +  K  L I DS  SD FSLDTVGS G V CK K + Y+VGV I  SS  LT+IVTF P++M+ N+S+  L   E   +   I V   + VPFWP +   K ++   +VE  +    + +   + +S L+ L  +F GI V+V + + + +I    Y  G A   +IN +  +    F+     ++  LP  + V+YAW+D   +R+  W     K Q   + T D +E   + +   Y VSF+    RILLFT +  V      +   E  +QEI ISL +IG+SLV+N  +QE++YI IT+S V W + ++K+R KPL + Q+E LE AF E+     T  +  +  IV ++      L  D S    I+P    + R F P + L+Y  S HQ  + L++Y+IQI NQ     FP VF P  PP+SV LD+  KPL ++S V+  +G +   R KYF+VLIQEM L +D GF+ ++ D  S  G VP      +F KD +  +  L   S+  T        V++ FHISPIK+HLSFSLS     G      K  +  + LNL L+S+G  +TD+QDV+FKL YFE QN      ++   +  HY  QA++QMYVLVLGLDVLGNPFG  R +++GVE  FYEP +GA+ GPEEFVEG+A GVK+L G AVGG AGA SRI G M KG+AA+T+D +YQ+KRRE + K+P  +  G+ +GGKGL+ GFV G+TG+VTKPI GA+K+G  GFFKGVGKGLVG VTRP  G++D ASS+F+GI++  D++ +V  +RPPRFI +DG+IR Y +RE  G+ +  KI      KC       Y  H    S  +++ +    +  + R    G  + +WQ ++++    P I   +  +    KE+ + +F      K I
Sbjct: 2027 KPKNFLQNNKVQLMITDSELSDPFSLDTVGSHGAVKCKSKKLDYQVGVTIYASSFNLTRIVTFTPFFMVANRSNYTLSVAE-EGSEKCITVSTEQCVPFWPENSNNKLIV---QVEGSKAAPKKIYFNKQDNSTLLYLGDQFGGILVDVTLRDHSTVITFSDYHAGAAPFLIINDMKSETVTFFQSSVNEEKDCLPPGKAVLYAWKDPTSERKLTWICR--KSQGEVSQTEDRVEYLRLEDTSVYLVSFLEGLQRILLFTENESVFNVTFDNQKAELAEQEIIISLSNIGISLVNNYTKQEVSYIGITNSDVVW-ETKKKSRWKPLSIKQTEKLEQAFKEY-----TESSPTDTKIVALDTNCQVCLTPDFSGMKFIQPTTDPIRRSFLPALKLEYSASAHQTSIRLQVYRIQIQNQNPAAIFPFVFYPVKPPKSVTLDSEPKPLTDVSIVMRTAGHSEISRIKYFKVLIQEMDLRIDLGFLYALADLFSQSGDVPKDQAIELFKKDVESFQTELSAVSSTDT----SSISVYEYFHISPIKLHLSFSLSTGGDDGNKEQRDKELIPVQSLNLLLKSIGATLTDVQDVVFKLAYFELQNQFYTTQELQWEVIRHYSKQAIKQMYVLVLGLDVLGNPFGFFRDLSEGVEAFFYEPYQGAIQGPEEFVEGMALGVKALVGGAVGGLAGAASRITGAMAKGVAAITMDEEYQQKRREAMNKQPSGIREGITRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVTRPTGGIIDMASSTFQGIKKATDSSDDVESLRPPRFIHEDGVIRPYRMREGAGSQMLQKIENGRFAKCK------YFAHAMVNS-SDLLMVMKSGVLFVTRG-TFGQLTCEWQYSFDEFTKEPFIVSGRR-LRIEAKERVKSVFHAKEFGKII 2927          

HSP 2 Score: 457.603 bits (1176), Expect = 3.024e-128
Identity = 278/779 (35.69%), Postives = 442/779 (56.74%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPET-TNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQK-KVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQ-SKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSE-RTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKI 2348
            MVFESLVV+++N++LGDYVV LD SQLKLG+ GG                             KL LKIPWK+LY++PV A LDG+Y+LI+P + +KYD EKE     E KQ+EL RIEE K K  D   + E +D+F EKL  Q++KNLQ+ ISNIHIRYED  T P    + G++L NLS QT D +     + ++S + F+K V++D+L  YWN     F   +  T   I+K  I++         F+ +PIS  A +   M P +  +F+ P++ LD+ L+ I   FN+ QY  +   L+S+D       +RK+RP+V + +N  IWW +    ILE  ++ R  MWSWK I+ HR + + Y +++  K+  KK S+      +  E+ LD+ NITL RQQAEM+A K   K+ +  S     +           S   ++  ++ +   +    + EEK +LY AIGYSE   DP +P  Y +++L ++L  +   + ++  K  +L+   I  +S  ++QR  A AI     +   +VTGT   + SVP ++S++      ++LL + FETNPLD+ A+QK+ + ++PLEIIYD+ T+N++V F  PP+++ L ++++  +  +EE ++ T+TGL ++ + +   D+K+++  SY I+PE G Y +   +LL+DLGS+ +VS+ R+ +P          K G+ + + +   AYD F + LS +Q L   + ++W+K R+ + S  HIL P   +    + ++  DA +P +
Sbjct:    1 MVFESLVVDVLNRFLGDYVVNLDSSQLKLGIWGG-----------------------------KLDLKIPWKNLYTQPVEAVLDGVYLLIVPTASIKYDAEKEEKQSLEAKQRELQRIEEAKQKVAD---QEEKQDTFVEKLITQVIKNLQVKISNIHIRYEDDITNPKCPLSFGVSLQNLSLQTSDKNWNP-CLHDESAKLFYKLVRLDNLFAYWNVKSDMFYHRSYDTALEILKYGIAARNTIPEGYDFVFRPISGRAKL--RMNPRSDVDFSSPKIDLDVNLQDIAIDFNKPQYHSVMELLESIDLMSRNLPYRKFRPDVCVATNAPIWWKYAITSILEVNIKPRLHMWSWKRIRKHRLQVKKYKELYRMKITSKKPSEEVLSKTEEYEKALDIFNITLARQQAEMEACKAGLKIYRPGSKDEDCNSKGWFGWMWGWSGETAEKKEDFKSGSLEGLMSPEEKVRLYAAIGYSETAVDPTLPKTYEAMRLCVKLLSMSIQLREDKGKT-ELINFAITDLSTVLKQRPGAQAIKFEAQIGTFEVTGTP-QEKSVPCVLSSRNLIENNISLLSLMFETNPLDERADQKLQVESQPLEIIYDAKTINNLVDFFRPPQDVHLEQLASATMTKLEEFRDRTSTGLLYVIETQKVLDLKINLMASYIIVPENGFYEQSSNLLLLDLGSLKMVSKSRSHLPQL--------KIGQSSIEDIMSRAYDSFDVQLSKMQLLY--RKNNWKKARNLKRSSLHILEPMDLKVEFSRAMVVTDARMPNL 732          

HSP 3 Score: 308.145 bits (788), Expect = 8.784e-83
Identity = 352/1509 (23.33%), Postives = 652/1509 (43.21%), Query Frame = 3
Query: 2763 VLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI-----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGD--ANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIK--NDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRK-KPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQG-SLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYIS-----------CDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYL--------GMKQLELRSVLQVFNNTDDVITLY----YLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQ--IVNSSETLRTYKLDINVPL----ALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVT-YHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDA 7166
            V+ D +   + K+  K ++ L +  +GT L ++ FDM+  A++  + L  PQ+   + K+ VC      HT G       L   +   P+F T + N    ++  F S+++ LH EALL  ++F+ SL+P     + E+ E       +  +     ++E +    +K+ K D        D ++L              +E S    +  G+K  I EI++                              G+ + V  +   TEV   + ++ I D  P A +KK + I+  E  FS ++  +   T    Y  D+S VD  + + VG I++IF+  FV  I  F+N+FQ + Q +A      +E AA+ +  + +   S R  LDI+VKAPV+ +P++SKS   L+ DLG +T  N F+I       +   V++   I LS L++  ++    ++++E  +L P+N+ + + RNL+ +  H +P I I   +  ++  ++         VL S F        +V E+   VK +                    T++  F+ + L + L+ G    + +E  S +     L + K   +S+   M  +  +  +V+L+N  +LD +  S +K    +  +K   + +  + + + Q +   + L V +  +Y+C  +++L+ + D F                 ++  +++ +  +  K  ++PV+  ++  E++V V  PEI+ V D+   D+ A++V T   L      E   +   + D     CPF S+ R      I+ P  +  + +   N+G Q   L+++ L++ ++P +I ++  I + +++         +   ++     +  + W+K+   + +  F                               E + ++ K +   N+    E+ + + +  I V LE+ V  + +PML+ + S  G++ N  T I L   + + V  FN+   VWEPL+E  P E ++  SF    +            C+  G  +EE+++    I     +I + S+D L +T+SK  +  L NL  AF DA S   +  K I  AP+VVKN  G  ++  ++     KP NS    K     DGE    D V  K         ++  +F    S+S ++  I   ++  L  S  D    ++   S   +  L+     Y+        G K + +RS LQ+ N+    I +Y     L   S + E                +N+P+ +  + +   +  V++   E+S   +  ++ KN    D  +L  K  +      S+ L+I  V+  +++S  T   + L   V L     L N LP +I +  Q+   ++  GC   +  A L   + +K+   +Y  + W     +  K +++  INF+      +S +D+ VH+    GQ  + ++SPYWM+NKTG  L YKA DD   +H A
Sbjct:  723 VVTDARMPNLSKDVEKTVLHLEIMGLGTELNVRTFDMSANAFLREISLKCPQY---TGKNSVCTLSV--HTDG-------LYTADINGPEFKTTYKNVEQLIKVNFSSLDIHLHTEALLSTMSFLTSLVP-----QSERKEM-----DQPVQDSEEEKREEQKKTTIKKSKYD--------DVVNL-----------HVCAELSCLRVFIRGQKCRISEISI-----------------------------EGLDSQVVITKKATEVLAKLKNIAILDCDPEANFKKAVCIKGKE-FFSFKMVSYVGATEGDAYR-DMSVVDSHITLNVGCIQVIFVNKFVSAILAFINNFQATKQALAEATVHAAEKAASGVKELAQH--SFRLALDIHVKAPVVIVPQSSKSSQVLVADLGLITVNNKFSIVIPKSHSNLPPVIDSMMINLSELKM--TVYENGSLQSELQLLQPVNLDITIERNLAADWYHDVPDIKIIGHLKPMNKKINS--------VLLSFFKGTTVVTAAVVETYSPVKIK-------------------TTLKLDFQFDSLTLNLYSGKTFGSVHERDSSLK----LAEFKLGLISLAVKMSSDGTVNAAVKLTNC-LLDDKRQSVQKATPRMLEMKVGTEKNVMVDVSYKQGRE-GTILDVTVRDVYLCASMEFLLTVADIF-----------------LKASQQSSKAEKSIKQTSAPVKESVSKMEMNVVVKNPEIVFVADLSRADAPALVVTTQCELCIKTSPEMQNMTAAVKDLHMKACPFLSDQRAGKITTILQPCDL-FFQSIQSNSGPQMIELSIKSLMLKVSPVIINTVITITSAISS---------NKEAVSEVTSPVSPDLWKKKNLKDLKMWFFE-----------------------------ESKESENKPEESSNMVLTGET-LKMSIESIIVTLEAGVGHRTLPMLLAKSSFSGNVTNWSTLINLYCQLELEVHCFNEMFGVWEPLVE--PLEVDKTDSFRPWNLGVKMKKKAREQICEPDG--EEENYK----IPEYITAISIFSKDHLNITLSKCGMATLGNLANAFTDAASKKCDACK-IDQAPFVVKNYLG--LLVSVSPSDSFKPLNSAAGSKKIDLHDGECLDMDYVQSK---------SQTDQFSAMTSLSSKHFYI---QLTPLDHSHTDKIPLTKVGRSLYCVRHLVSGIERYIVCQVDSVEGTKCITIRSPLQIRNHFSIPIGIYEEGVCLGTASPEDE----------------FNIPLAS-YSRSVLCFKPVQEKKYEMSEGVSFEDVIKN----DGLLLNMKCQSATSKKDSLKLNIVPVKDIVMSSDHTNEGWHLPYVVHLWPTVVLRNLLPYQIKFSLQV---NLDDGCSTQIHNAALD-NTKLKLQLLNYTDQGWTAEYHIQSKQQDIHFINFRCVAELDKSELDIAVHVTYTTGQTIIALHSPYWMVNKTGRMLQYKA-DDIHRKHPA 2016          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Xenopus
Match: VPS13A (vacuolar protein sorting 13 homolog A [Source:NCBI gene;Acc:100488998])

HSP 1 Score: 546.199 bits (1406), Expect = 3.167e-155
Identity = 376/926 (40.60%), Postives = 534/926 (57.67%), Query Frame = 3
Query: 7368 KNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEG-----LEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVS--GSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTG------KTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITE----KCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEI 10094
            K + F +  K  L I DS  SD FSLDTVGS G V CK K + Y+VGV I  SS  LT+IVTF P++M+ N+S+  L   E   +   I V   + VPFWP +   K ++   +VE  +    + +   + +S L+ L  +F GI V+V + + + +I    Y  G A   +IN +  +    F+     ++  LP  + V+YAW+D   +R+  W     K Q   + T D +E   + +   Y VSF+    RILLFT +  V      +   E  +QEI ISL +IG+SLV+N  +QE++YI IT+S V W + ++K+R KPL + Q+E LE AF E+     T  +  +  IV ++      L  D S    I+P    + R F P + L+Y  S HQ  + L++Y+IQI NQ     FP VF P  PP+SV LD+  KPL ++S V+  +G +   R KYF+VLIQEM L +D GF+ ++ D  S  G VP      +F KD +  +  L   S+  T        V++ FHISPIK+HLSFSLS     G      K  +  + LNL L+S+G  +TD+QDV+FKL YFE QN      ++   +  HY  QA++QMYVLVLGLDVLGNPFG  R +++GVE  FYEP +GA+ GPEEFVEG+A GVK+L G AVGG AGA SRI G M KG+AA+T+D +YQ+KRRE + K+P  +  G+ +GGKGL+ GFV G+TG+VTKPI GA+K+G  GFFKGVGKGLVG VTRP  G++D ASS+F+GI++  D++ +V  +RPPRFI +DG+IR Y +RE  G+ +  KI      KC       Y  H    S  +++ +    +  + R    G  + +WQ ++++    P I   +  +    KE+ + +F      K I
Sbjct: 2202 KPKNFLQNNKVQLMITDSELSDPFSLDTVGSHGAVKCKSKKLDYQVGVTIYASSFNLTRIVTFTPFFMVANRSNYTLSVAE-EGSEKCITVSTEQCVPFWPENSNNKLIV---QVEGSKAAPKKIYFNKQDNSTLLYLGDQFGGILVDVTLRDHSTVITFSDYHAGAAPFLIINDMKSETVTFFQSSVNEEKDCLPPGKAVLYAWKDPTSERKLTWICR--KSQGEVSQTEDRVEYLRLEDTSVYLVSFLEGLQRILLFTENESVFNVTFDNQKAELAEQEIIISLSNIGISLVNNYTKQEVSYIGITNSDVVW-ETKKKSRWKPLSIKQTEKLEQAFKEY-----TESSPTDTKIVALDTNCQVCLTPDFSGMKFIQPTTDPIRRSFLPALKLEYSASAHQTSIRLQVYRIQIQNQNPAAIFPFVFYPVKPPKSVTLDSEPKPLTDVSIVMRTAGHSEISRIKYFKVLIQEMDLRIDLGFLYALADLFSQSGDVPKDQAIELFKKDVESFQTELSAVSSTDT----SSISVYEYFHISPIKLHLSFSLSTGGDDGNKEQRDKELIPVQSLNLLLKSIGATLTDVQDVVFKLAYFELQNQFYTTQELQWEVIRHYSKQAIKQMYVLVLGLDVLGNPFGFFRDLSEGVEAFFYEPYQGAIQGPEEFVEGMALGVKALVGGAVGGLAGAASRITGAMAKGVAAITMDEEYQQKRREAMNKQPSGIREGITRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVTRPTGGIIDMASSTFQGIKKATDSSDDVESLRPPRFIHEDGVIRPYRMREGAGSQMLQKIENGRFAKCK------YFAHAMVNS-SDLLMVMKSGVLFVTRG-TFGQLTCEWQYSFDEFTKEPFIVSGRR-LRIEAKERVKSVFHAKEFGKII 3102          

HSP 2 Score: 478.019 bits (1229), Expect = 1.907e-134
Identity = 293/805 (36.40%), Postives = 459/805 (57.02%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETK-DSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPET-TNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQK-KVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQ-SKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSE-RTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELI 2423
            MVFESLVV+++N++LGDYVV LD SQLKLG+ GG                F  P          L LKIPWK+LY++PV A LDG+Y+LI+P + +KYD EKE     E KQ+EL RIEE K K  D +K  E K D+F EKL  Q++KNLQ+ ISNIHIRYED  T P    + G++L NLS QT D +    +  ++S + F+K V++D+L  YWN     F   +  T    +          ++ + +L +PIS  A +   M P +  +F+ P++ LD+ L+ I   FN+ QY  +   L+S+D       +RK+RP+V + +N  IWW +    ILE  ++ R  MWSWK I+ HR + + Y +++  K+  KK S+      +  E+ LD+ NITL RQQAEM+A K   K+ +  S     +           S   ++  ++ +   +    + EEK +LY AIGYSE   DP +P  Y +++L ++L  +   + ++  K  +L+   I  +S  ++QR  A AI     +   +VTGT   + SVP ++S++      ++LL + FETNPLD+ A+QK+ + ++PLEIIYD+ T+N++V F  PP+++ L ++++  +  +EE ++ T+TGL ++ + +   D+K+++  SY I+PE G Y +   +LL+DLGS+ +VS+ R+ +P          K G+ + + +   AYD F + LS +Q L  +  +DW+K R+ + S  HIL P   +    + ++  DA +PK  + G LP L + ISD+ L+ VLELI
Sbjct:    1 MVFESLVVDVLNRFLGDYVVNLDSSQLKLGIWGGE------------LSTFSSP----------LDLKIPWKNLYTQPVEAVLDGVYLLIVPTASIKYDAEKEEKQSLEAKQRELQRIEEAKQKVADQEKPKEEKQDTFVEKLITQVIKNLQVKISNIHIRYEDDITNPKCPLSFGVSLQNLSLQTSDKNWNPCL-HDESAKLFYKLVRLDNLFAYWNVKSDMFYHRSYDTALVSL----------VSLICYLFRPISGRAKLR--MNPRSDVDFSSPKIDLDVNLQDIAIDFNKPQYHSVMELLESIDLMSRNLPYRKFRPDVCVATNAPIWWKYAITSILEVNIKPRLHMWSWKRIRKHRLQVKKYKELYRMKITSKKPSEEVLSKTEEYEKALDIFNITLARQQAEMEACKAGLKIYRPGSKDEDCNSKGWFGWMWGWSGETAEKKEDFKSGSLEGLMSPEEKVRLYAAIGYSETAVDPTLPKTYEAMRLCVKLLSMSIQLREDKGKT-ELINFAITDLSTVLKQRPGAQAIKFEAQIGTFEVTGTPQ-EKSVPCVLSSRNLIENNISLLSLMFETNPLDERADQKLQVESQPLEIIYDAKTINNLVDFFRPPQDVHLEQLASATMTKLEEFRDRTSTGLLYVIETQKVLDLKINLMASYIIVPENGFYEQSSNLLLLDLGSLKMVSKSRSHLPQL--------KIGQSSIEDIMSRAYDSFDVQLSKMQLLYRKNNEDWKKARNLKRSSLHILEPMDLKVEFSRAMVVTDARMPKCKLSGELPILSIQISDQKLKGVLELI 760          

HSP 3 Score: 317.005 bits (811), Expect = 1.997e-85
Identity = 366/1526 (23.98%), Postives = 664/1526 (43.51%), Query Frame = 3
Query: 2781 NTLVEKNGAKEIVKLIVH----EIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITM----TQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI-----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGS------------ARTASDESI--TVQFGFKMEFLDVELFKG-----DANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIK--NDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQG-SLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYIS-----------CDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYL--------GMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQ--IVNSSETLRTYKLDINVPL----ALHNQLPIKISYQSQIFSHD---IKPGCLELVRTACLKVQSNIKVT-YHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDA 7166
            ++L +KN +K++ K ++H     +GT L ++ FDM+  A++  + L  PQ+ D   K PV +  T  +T   +LL +  +  +   P+F T + N    ++  F S+++ LH EALL  ++F+ SL+P     + E+ E       +  +     ++E +    +K+ K D        D ++L              +E S    +  G+K  I EI++    +Q  IT   +K   ++A  K+  ILD                                P A +KK + I+  E  FS ++  +   T    Y  D+S VD  + + VG I++IF+  FV  I  F+N+FQ + Q +A      +E AA+ +  + +   S R  LDI+VKAPV+ +P++SKS   L+ DLG +T  N F+I       +   V++   I LS L++ R++    ++++E  +L P+N+ + + RNL+ +  H +P I I   +  +++ + Q D  +    L  N  E     P+  ES +E      L   D S S              T S   I  T++  F+ + L + L+ G       + +E  S +     L + K   +S+   M  +  +  +V+L+N  +LD +  S +K    +  +K   + +  + + + Q +   + L V +  +Y+C  +++L+ + D F+KA     +            EK+ ++       S    +++  E++V V  PEI+ V D+   D+ A++V T   L      E   +   + D     CPF S+ R      I+ P  +  + +   N+G Q   L+++ L++ ++P +I ++  I + +++         +   ++     +  + W+K+   + +  F                               E + ++ K +   N+    E+ + + +  I V LE+ V  + +PML+ + S  G++ N  T I L   + + V  FN+   VWEPL+E  P E ++  SF    +            C+  G  +EE+++    I     +I + S+D L +T+SK  +  L NL  AF DA S   +  K I  AP+VVKN  G  ++  ++     KP NS    K     DGE    D V  K         ++  +F    S+S ++  I   ++  L  S  D    ++   S   +  L+     Y+        G K + +RS LQ+ N+    I +Y            +  + L        +N+P+ +  + +   +  V++   E+S   +  ++ KN    D  +L  K  +      S+ L+I  V+  +++S  T   + L   V L     L N LP +I +  Q    D   +  GC   +  A L   + +K+   +Y  + W     +  K +++  INF+      +S +D+ VH+    GQ  + ++SPYWM+NKTG  L YKA DD   +H A
Sbjct:  840 DSLRKKNLSKDVEKTVLHLEIMGLGTELNVRTFDMSANAFLREISLKCPQYTD-DEKRPVYIITTLDNTT-DDLLTLEYIKADINGPEFKTTYKNVEQLIKVNFSSLDIHLHTEALLSTMSFLTSLVP-----QSERKEM-----DQPVQDSEEEKREEQKKTTIKKSKYD--------DVVNL-----------HVCAELSCLRVFIRGQKCRISEISIEGLDSQVVIT---KKATEVLAKLKNIAILDC------------------------------DPEANFKKAVCIKGKE-FFSFKMVSYVGATEGDAYR-DMSVVDSHITLNVGCIQVIFVNKFVSAILAFINNFQATKQALAEATVHAAEKAASGVKELAQH--SFRLALDIHVKAPVVIVPQSSKSSQVLVADLGLITVNNKFSIVIPKSHSNLPPVIDSMMINLSELKMYRTVYENGSLQSELQLLQPVNLDITIERNLAADWYHDVPDIKIIGHLKPMNLILGQEDLTLILQTLNENLGETSELFPA--ESDDET-----LVSGDNSSSHSAGTTVVTAAVVETYSPVKIKTTLKLDFQFDSLTLNLYSGKTFVLQGSVHERDSSLK----LAEFKLGLISLAVKMSSDGTVNAAVKLTNC-LLDDKRQSVQKATPRMLEMKVGTEKNVMVDVSYKQGRE-GTILDVTVRDVYLCASMEFLLTVADIFLKASQQSSKA-----------EKSIKQTSAPVKESAPTQQVSKMEMNVVVKNPEIVFVADLSRADAPALVVTTQCELCIKTSPEMQNMTAAVKDLHMKACPFLSDQRAGKITTILQPCDL-FFQSIQSNSGPQMIELSIKSLMLKVSPVIINTVITITSAISS---------NKEAVSEVTSPVSPDLWKKKNLKDLKMWFFE-----------------------------ESKESENKPEESSNMVLTGET-LKMSIESIIVTLEAGVGHRTLPMLLAKSSFSGNVTNWSTLINLYCQLELEVHCFNEMFGVWEPLVE--PLEVDKTDSFRPWNLGVKMKKKAREQICEPDG--EEENYK----IPEYITAISIFSKDHLNITLSKCGMATLGNLANAFTDAASKKCDACK-IDQAPFVVKNYLG--LLVSVSPSDSFKPLNSAAGSKKIDLHDGECLDMDYVQSK---------SQTDQFSAMTSLSSKHFYI---QLTPLDHSHTDKIPLTKVGRSLYCVRHLVSGIERYIVCQVDSVEGTKCITIRSPLQIRNHFSIPIGIY------------EEGVCLGTASPEDEFNIPLAS-YSRSVLCFKPVQEKKYEMSEGVSFEDVIKN----DGLLLNMKCQSATSKKDSLKLNIVPVKDIVMSSDHTNEGWHLPYVVHLWPTVVLRNLLPYQIKFSLQGVDVDATLVDDGCSTQIHNAALD-NTKLKLQLLNYTDQGWTAEYHIQSKQQDIHFINFRCVAELDKSELDIAVHVTYTTGQTIIALHSPYWMVNKTGRMLQYKA-DDIHRKHPA 2191          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Xenopus
Match: git2 (G protein-coupled receptor kinase interacting ArfGAP 2 [Source:Xenbase;Acc:XB-GENE-949238])

HSP 1 Score: 542.347 bits (1396), Expect = 6.583e-154
Identity = 368/931 (39.53%), Postives = 555/931 (59.61%), Query Frame = 3
Query: 7305 AEDLH-DHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG-----WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPF-YESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINE-MKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILD-FVSGSVPTYDEE--AMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLS----RVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEG------NNIWI-KITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTI 10031
            AED+H  H        + +   KN  F+K  K  L I+ S WS  FSLDTVGS G V C    + Y VGV I +SS  L++IVT  P+Y ++N+S + +   E+  +       W  V   E +PFWP +  GK  +     E     +++PF F  + +  L+ LE    GI V+V I + + +I    Y +G A   +INH+    ++ + Q  T  E  L   +  ++AW+D    ++  W+     ++   +L  D    F  N+ ++ +WVSF+    R+LLFT D+ V   AR + ++E  DQE+T+SLHS+GLSLV+N  + E++YI I SSGV W +++RK++ +P    Q   LE A+++++   +     VEE+ V V   +V +  +L   P KC + R F  G++++++ SPHQ  +  ++Y +Q+DN +    FPVVF P  PP+SVALD+  KP I++S +   +  +  M++KYF VL Q M L LDQGF+ +++  F   + P  +++   +  KD   +   L+E+S             F++FHISP+K+HLS SL     + N+  K  +    +NL L+S+G  +TD+ D+IFKL  FE +      NQ+ + +  HY  Q L+Q+YVLVLGLDVLGNPFG+IRG+ +GVE  FYEP +GAV GPEEF EG+  G+KSL GH VGGAAG VSRI GT+GKG+AA+T+D +YQ+KRR+++ ++P++ G  LA+GGKG + G V GVTG++TKP++GAKK+G  GFFKG+GKGLVGVV RP  G+VD ASS+F+GI+RVA++  EV  +RPPR I +DGIIR Y   EA+G      N+ W+ +  E+      + Y++ +  K     +F+T +R+  +   EILG   I W   +E+   PP ++ +  T+
Sbjct: 2494 AEDIHVKHPSDYRDVILFSFKKKN-IFSKN-KIQLCISTSSWSTGFSLDTVGSYGCVKCPAVKMEYLVGVNIKMSSFNLSRIVTLTPFYTLVNKSKLEIEVGEMATDVSPTDIKWNYVSSSECIPFWPENISGKLCVRVIGCEG----ISKPFFFNKQDNGTLLSLESMNTGIIVDVSIADHSTVITFSDYYKGAAPALIINHM-PHAKLTYNQSGTEIEKELGPGEARLFAWEDPTGTKKLNWKCASTTRE--VDLLKDESGQFAYNQNVQIHWVSFLDGRQRVLLFTEDVAVVTKARQAEEMEQSDQEVTVSLHSLGLSLVNNDSRHEISYIGIISSGVIW-EMKRKHKWQPFNQKQIFQLEGAYEKYLTNEEQGWVSVEENFV-VCFSKVPMEMRL---PIKCSIRRNFMSGIYMEFKQSPHQRSLRAQLYWLQVDNHLPGSMFPVVFHPVIPPKSVALDSEPKPFIDVSIITRFNEFSKVMQFKYFMVLTQCMALKLDQGFLAAVIGLFTPETDPNAEKQRTQLIQKDLDSLNTELMESSLTDISFPS----FFEHFHISPLKLHLSLSLGAGGEQSNKEEKEIIAIASVNLLLKSIGATLTDVDDLIFKLACFELKYQFYKQNQLRNLVVRHYTEQFLKQVYVLVLGLDVLGNPFGLIRGLTEGVEAFFYEPFQGAVQGPEEFAEGLVIGMKSLLGHTVGGAAGMVSRITGTVGKGLAAITMDKEYQQKRRQEMGRQPKDFGESLAKGGKGFLRGVVGGVTGIITKPVEGAKKEGAAGFFKGIGKGLVGVVARPTGGIVDMASSTFQGIQRVAESTEEVSPLRPPRLIHEDGIIRPYNESEAQGFKLFQLNDQWLPRFKER------QRYLYKILTKVS---LFIT-RRLICMKEVEILGHRMIDWTYTFEEFVHPPIVDGNTLTL 3396          

HSP 2 Score: 358.607 bits (919), Expect = 4.091e-98
Identity = 271/822 (32.97%), Postives = 423/822 (51.46%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRND---PKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAI-MKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFN-RNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNI-SIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLAS--DGSDEL--QKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQ-TAPP 2456
            ++ ES+V +L+N++LGDYV  LD+SQLKLG+ GGN  LENL IKENA   + + V        KLTLKIPWK+LY + V+A L+GL++L++P + +KYD +KE   + ETKQ+EL RIEE   K  D   PK     KD+F EKL  Q++KNLQI I++IHIRYED   C +            S QTC+ S    I+ E +T+  +K V + +L+V    +      + R  L    M  N               +P++ SA ++++          P   ++LK  +I +    +N   ++T                  +P V    N  +  W +  N ILE  ++RR  MWSW  I  HR   ++Y + ++ KL   KL +     ++ CE+ LD+ NI L RQQA+ +  +  +KL  K SS G      G   +  S   A+  D  DE+   +K+    T EEKAKL+ AIGYS+ + +  +P  YV+  LN +L +    I ++ +   + +++ I  +S  V QR  A A+ +   + +  VTG       VP+LV T   ++  +LL + FETNP    A+Q + + ++P+EIIYD+ T+N + +F    + + L ++++  L  +EE+KE TATG+             +D     FIL          +++ VD       +E T+                L+ + + E AYDKF + +  +Q L   +G+DW+K R + SS  HIL+P   E  L+K ++  D+ +P+  + G LP L + ISD+ +Q V +L+  IP PQ T+ P
Sbjct:    1 IMLESVVADLLNRFLGDYVENLDRSQLKLGIWGGNVALENLRIKENALVKYIVFVLS-----DKLTLKIPWKNLYGDAVVATLEGLFLLVVPGASIKYDAQKEEKSIQETKQRELLRIEEALQKAADKDIPKD--AKKDTFMEKLLTQVIKNLQIKITDIHIRYED--DCHDICTVYSHIFHLYSTQTCNESWKPCILNE-ATKIIYK-VSIYNLSVINMLHNCSCQCKTRMQLYIFDMPQNRE-----------FFKPVNASAKLYIN----------PLAEMELKTPKIDWIMEVQNVAIEVT------------------KPQVSYILNCNTTQWLYAINSILEVHIKRRTHMWSWNNIAKHRQNLKMYKNAYKTKLTQAKLPEETVKQLQACEKLLDVFNIVLARQQAQAEIIRSGQKLMAKKSSAGGEKKGGGWFGSFWSKKEATKKDDVDEIFASEKIDDFMTPEEKAKLFTAIGYSDSSHNLALPKAYVAHILNFKLLRTSITIRED-IHTPETLQIQIIDLSTKVFQRPGAQAVKIEAKLEHWYVTGL-SQQNIVPSLV-TSIGNRESSLLRIVFETNPQHIKADQVLILESQPVEIIYDAVTINALAEFFQTQKGMDLEQLTSATLMKLEEIKEKTATGI-------------ID-----FIL---------LQIISVDQ------AEETT---------------NLSLEDLMEKAYDKFDVRIKNVQILFGTKGEDWKKAREKGSSSLHILQPLDIELKLEKAMVERDSRMPRFKILGELPLLHIKISDKKIQGVFQLVNSIPLPQKTSTP 721          

HSP 3 Score: 307.76 bits (787), Expect = 1.115e-82
Identity = 352/1484 (23.72%), Postives = 636/1484 (42.86%), Query Frame = 3
Query: 2844 TTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFA----IDQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDI-AGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMV--QTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESK--IYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLE-VYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEP-SAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFY--INKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRG--------NVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPT-----LLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSI--EEVQIVNSS---ETLRTYKLDINVPLALHNQLPIKISY--QSQIFSHDIKPG-CLELVRTACLKVQSNIKVTY--HYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESF-MDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAV 7184
             ++ LK+  MA   ++G  I AV                   HT+    L++     ++  P+F T+H NT         S++  LH +ALL ++NF++S +P  NI+EE +        KE   K++ L+                SI+                     A  E  ++N  DS               ++A L  L + V +E   NI DI I GM A V      TEV   + D  I D     ++KK ++I  DE +F+ +++++ + T    Y  D+S VD  + + VG I II+L+ F++ +  FL+ FQ + + ++    + +E AA+ +    ++  S R  +DI++KAPVI IP++S S NA++ DLG +  +N F+    +D     V+++  I L+ L++SR++L+  + K E  IL P+N+ + + RNL+ +    IP + +   +  + + +SQ D ++   VL  N  E     PS+P   E  KS        +  +A    +ES+                                  L +++   L+  G+M+ + +M+ SV L    + D +    K  + +I   K+   +   +     + +   LI  I   +Y+C  +++LM + DFF+           +AM      EK+ +K+ ++  + P  P      + V +  PEI+ V +++  D+ A+ V  Q NF L    +  S  +   + +F+ + CPF  E R  N   ++ P S+ +             L++E +II ++P ++ ++  I++ L  +  +E          N  +L+  N W+ R  D     F+                   G D+AQ + +  Q              EHKE +    L +  +++ LE  +  + VP+L+ E +    +KN  + I +    ++ V Y+N++  VWEPL+E V          FE++     D  LF  +DF  ++  EP SAI+I   S+D + +T+SK  L V  NL KAF +  +   + + S   AP  +KN  G  ++     N   ++   +S              Q +D VDE QSL  E    +++K G   ++ R+  S+    +    ++   N   ++         N  +    +++V   A  G K + +RS LQ+ N+     T+Y     +   E        EE      ++VP+ +      L   G +    K+     S S   W    +        L C ++        V  S   +E+  ++ +   E    Y + +   L + N +P  I +  +    +H++  G  L+++++   ++   I   +   YQ + W G + +   + E   + F A    SE   +DL VH+     +  +++YSPYW++NKT   L YKA +D  V+H +   D +
Sbjct: 1245 ASVYLKKISMACNDFMGEEIPAVI------------------HTRKCFFLYLQ---ADQNGPNFKTIHDNTEQSFNISVSSLDFILHTQALLSLMNFLSSAVPSHNILEENRP-------KESKAKESRLK----------------SII---------------------ARPESYNFNTEDS-----------FSLKVSAKLNALNIFVCDEI-CNIADIRIQGMDASVAVQTKQTEVCARLEDFVIFDVDSKTIHKKAVFIMGDE-VFNFKMSMYPEATEGISYS-DMSAVDGKICLRVGCIHIIYLHRFLMSLLNFLDKFQAAKEALSTATSQAAEKAASSMKEFAQK--SFRLSMDIDLKAPVIIIPQSSVSANAIVADLGFIRIQNKFSLVPVVDCPLPPVVDQMNIQLTELQLSRTVLMSGSDKPELEILQPVNLLVSVQRNLASSWYSEIPALQLVGDLKHMQIALSQEDLSILMSVLFENLAEK----PSLPRVVETTKSI-------EQETALWLHNESLR---------------------------------LGELRLHLLTSSGNMYSDGSMDASVYLKTCTLDDLREGITKATSRMIDQ-KDGVCERNMVDITYKQGAQDCLITTILEKLYVCASMEFLMTVADFFI-----------QAMPQSPSGEKSMQKHVRQ--SHP--PSQKKVNVKVLIMDPEIVFVANLMKADAPALSVSFQGNFTLDS--LSGSQKVAAIVKEFKVLACPFLRELRGSNITTVMQPCSLQIEGTIDAAGAKNLILSLEEVIIKVSPIILNTVQTIIDALKPKPKEEEVNE------NLTELV--NIWDVRSIDSCSNWFL-------------------GVDMAQEITETFQS------------SEHKEKQESFELEVKSVQITLECGLGHRTVPLLLMESTFSTKVKNWTSFINITADTTLEVHYYNENYAVWEPLIERVNCGSRRWSLKFEMKNNPVMDKSLFPGDDF--ILIPEPRSAINI--YSKDTMNITISKCTLTVFGNLAKAFSEGTASTYD-YDSKDRAPLTIKNALGIPIIVQHGSNMRRNIPSPHS--------------QTHD-VDEGQSLELEFCAVELLKRGQHSALHRQDSSLLTVGIVPHGYTEMANIPVTKTARRLYNVRNPQQENFVSVLVQIDATEGNKVITIRSPLQIKNHFSIPFTVYKFVASAKLLEPIGVARQDEE------FHVPLDSYRCQLFLRPAGALEEQFKE-----STSYITWKEMVHRSSEVRCTLQCPASEISFLPLMVNCSAVPDEINFISRNGEKEWDPAYIIHLYPTLTVRNLVPYSIRFLLEGAAETHELAEGSSLDVLQS---RISGEIMELFLIKYQGRNWNGHIRVHENLSEFFPVLFTADS--SELLTVDLSVHVRHIGNRMVLSVYSPYWIINKTSRILQYKA-EDIHVKHPSDYRDVI 2509          

HSP 4 Score: 153.295 bits (386), Expect = 7.073e-36
Identity = 95/292 (32.53%), Postives = 168/292 (57.53%), Query Frame = 3
Query: 3447 ASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEA 4310
            A+L    V V +EK N+I +I I GM          TEV   + D+ + D +P  ++KK + I  +E +FS ++ L+ + T    Y+ D+S+VD  +++ VG I+I+FL+ F++ +  FLN+FQ + + V+    + ++ AA  + ++ E   S R  +D+++KAP+I +P++S S NA++ DLG L   N F +    DQ     ++   I L+  ++SR++L + + + +  ILHPINI + + RNL+    H IP + +   + +++VG+SQ D ++   VL  N  EA
Sbjct:  900 ANLDAFNVNVWDEK-NHIAEIKIQGMACHFFIQSNQTEVFARLKDIIVNDVNPQTIHKKAVSI-MEEEVFSFRLMLYPEATQGDAYK-DVSKVDGKLDLRVGCIQIVFLHKFLMTLLKFLNNFQAAKERVSMATAQAAQRAATSVKDLAER--SFRLLMDVHLKAPLIVVPQSSVSCNAIVADLGLLKINNRFTLVSSEDQQLPPFVDHMTINLTEFKLSRTMLQDGSFQPDVEILHPININLSINRNLAATWYHKIPVLEVKGHLNTMNVGLSQEDVSVLLNVLVENLGEA 1186          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Mouse
Match: Vps13a (vacuolar protein sorting 13A [Source:MGI Symbol;Acc:MGI:2444304])

HSP 1 Score: 720.309 bits (1858), Expect = 0.000e+0
Identity = 578/2046 (28.25%), Postives = 999/2046 (48.83%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETK-DSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFF-KSVKMDSLAVYWNSNPKPFS-SENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQV--LRAINVLPDSETASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIG--TTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTSV----VLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSN--FNEAINQVPSVPESKEEVKSRKILPKDDKSGSART---------ASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIR-NIKNDSSDFISIKFIQDKNSNSKLIVD--IAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTL--TAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPL---LEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIF---GAPYVVKNTCGKDV 6062
            MVFES+VVE++N++LGDYVV LD+SQL LG+  G   L+NL IKENA  + D+P KV+ GHI  L LKIPWK+LY++PV A L+ +++LI+P+S ++YDP KE   L ETKQ+EL RIE+ K K  D +K  E K D+F EKL  QI++NLQ+ IS+IHIRYED  T  ++  + GI+L N+S QT D      +   D+T +   K +++D+L  YWN N + F  ++   +LKA +KN I +         F+ +PIS SA + ++ + +  +F+ P+++L + L  I   FN+ QY  +   L+S+D       +RK++P+VP+  +   WW +  + ILE  V    RMWSW++I+ HR + + Y + +++KL  KK S     +++  E+ LD+ NIT+ RQQAE++AKK   K+ ++       +  +       S S A+   D     +    T EEK+ LY+AIGYSE   DP +P  + +LK  + L+ +  ++ +N  K  +L+ +V+  +S  V QR  A AI     +++  +TG    D   P L+S+   +   +LL + FE NPL+++  Q+  I AEPLEIIYD+ TVN IV+F  PP+++ L ++++  L  +EE +  TATGL ++ + +   D+++++K SY I+P+ G ++    +LL+DLG + + S+R S+       L   +  + + + +   AYD F I L++IQ L    GD+W++ R    S  HIL P      L K ++  D  +PK  + G LP + + ISD+ LQ ++EL+  IP      P+PV    +  R +   Q    P  +++   +  L       DSE     +            ++  ++   +    +KK  K   + N+I L   F +    +Q    +        G  E+  L +  +G  T    + FD+   A++  L L  P+++D   K PV L  T  +T  ++LL +  + VEK +P+  + + N    ++  F S+++ LH EALL  +N++N+++P L     EK+ + S+   E+                    K D  I+  +  ++    +  T++L AE            S  +IFI                       ++  NI +I I G+ +++      TE++  + ++ + D   +A+YKK ++I   E +FS ++  +   T+   Y  D+S VD  V++ VG I+++F+  F+  I  F+++FQ     +A    + +E AA+ +  +  +  SSR  LD+N+KAPV+ IP++  S N  + D G +T KN F     T      V++   I LS +R+ RS    +T +    +L P+N+ + + RNLSW     +P  +I A++  +   +SQ D       L  N  + + ++   S  +  E + S    P D    +  T         AS     +   F+ ++L + L+    +   +     P   L + K EN+     ++ + +   S  + N  + D +    K    +I   +  D  D + IK+ + K   + ++ D  +  +Y+C  V++LM +   F  A                      R  R+ P        L  +E+++ +  PEI+ V DM   D+ A+++ T   +       S+ +   I D Q   CPF    RK     ++ P  +     +   +     ++++ L + ++P +I +I  I + L  T ++  E +T +           + + W+K++    +  F+ +   E EK+   N+ +  G                                + LR+  I ++LE+ +  + VPML+ +    G+ KN L+ I L   + + V Y+N+   VWEPL   LE+   +  R ++  I+        + + +   +   +     +I   SRD L +T+SK  L +L+NL +AF +A +       S+F    AP+++ N+ G  V
Sbjct:    1 MVFESVVVEVLNRFLGDYVVNLDESQLSLGIWKGAVALKNLVIKENALHELDVPFKVKVGHIGSLKLKIPWKNLYTQPVEAVLEEIFLLIVPSSRIQYDPIKEEKQLMETKQQELKRIEKAKQKVFDKEKPREEKQDTFTEKLVTQIIQNLQVQISSIHIRYEDDITNGDKPLSFGISLQNISLQTTDQYWIPCL--HDNTEKLVRKLIRLDNLFAYWNVNSEMFYLNDYDESLKA-LKNGIVNENIVPEGYDFVFRPISASAKLQMNRRSD-FDFSDPKINLAVDLHTIAIEFNKPQYFSLMELLESIDMMTQNQPYRKFKPSVPLHLHAKEWWAYAIHSILEVNVCPSLRMWSWEHIRNHRYKMKRYREFYKKKLTSKKPSPEILMSLEELEKTLDVFNITIARQQAEVEAKKAGYKIYKEGVKDPEDNAGWFGWLWTWSESNANQQQDVKPGILEEMLTPEEKSLLYEAIGYSETAVDPTLPKTFEALKFFVHLKSMSIVLRENHQKP-ELLNVVVEGLSTSVVQRPGAQAIKFETKIDSFHITGLPD-DFKKPHLLSSLDDT---SLLQITFEINPLNETVAQRCTIEAEPLEIIYDARTVNSIVEFFRPPKDVHLAQLTSVTLTKLEEFRAKTATGLLYVIETQKVLDLRINVKASYVIVPQYGNFSPTSNLLLLDLGHLKVSSKRRSL-------LPDVRPSEASLEDIMHRAYDSFDIQLTSIQLLYSRVGDNWKEARKLNVSTQHILIPMHVNVELSKAMVFMDIKMPKFKISGKLPLVSLRISDKKLQGIMELLGSIP-----KPEPVTDVSAPARSFQ-IQASALPVSHISQKLIPLLEQPVTEDDSEEEFFDAPC-------SPLEECPQVSCRDKCTRQKKLQKKDCVMNLIQLRMRFEVAEVSIQFYHLV--------GDCELPVLEMGALGLGTEAEFRTFDLKGSAFLKELWLKCPEYLD-ENKKPVYLITTLDNTM-EDLLTLEFMKVEKNAPNLNSTYNNVLQLIKVNFSSLDIHLHTEALLNTMNYLNNILPEL----REKSASVSAAEPED--------------------KGD--IIKKLALKLPTNEDIITLQLLAEL-----------SCLQIFI----------------------QDQKQNISEIKIEGLDSEMIMKPLVTEINAKLRNIIVLDSDKMAIYKKALYITGKE-VFSFKMISYMDATAGYAYT-DMSVVDIRVHLTVGCIEVVFITKFLYSILAFIDNFQAVKDALAEATVQAAEMAADGVKELARK--SSRFALDVNIKAPVVLIPQSPVSQNVFVADFGLITMKNIFVTVTETQSNIPPVIDLITIKLSKMRLYRSQFRNDTYQEVLDLLLPLNLEVIVERNLSWEWYKEVPCFNIKAQLKPMEFILSQEDLTTVFQTLHGNIWYGQDLSAPSSANKDPETMTSGVTSPPDHSPATVVTAAVVEVHPQASQAHTMLNVSFQTDYLTMALYSPGPDEASFTDVRDPSLELAEFKLENIISSLKIYTDDSTVFSFSVKNCILDDKRSHVMKATPRMIGLTVGFDKKDMVDIKYRKIK---TFVVTDAVVQEMYVCASVEFLMTVAHIFFDAYMTSTALETSVQT---------RTTREAPAQ-----ELGKWEMNILIKNPEIVFVADMTRNDAPALVITTQCEICCKGEPTSNTVTAAIKDLQVRACPFLPVKRKGKVTTVLQPCDLFYQATQLGRDPQMIDISVKSLTLKVSPVIINTIITITSALYTTKETVPEENTSN-----------IAHLWDKKDTKNLKMWFLEES-NESEKVVPTNEVMPGG------------------------------ETLNLRIDSIFIVLEAGIGHRTVPMLLAKACFSGESKNWLSLINLHCHLELEVHYYNEMFGVWEPLLEPLEIDQTDDFRPWNLGIKMKKKAKEAIVESDSEAENYKVPEYKTAISFYSRDQLNITLSKCGLVMLNNLVEAFTEAAT----GSSSVFLRDLAPFMIFNSLGLTV 1881          

HSP 2 Score: 538.11 bits (1385), Expect = 6.834e-153
Identity = 404/1141 (35.41%), Postives = 599/1141 (52.50%), Query Frame = 3
Query: 6765 YKLDINVPLALHNQLPIKISY----------------QSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQSLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG--WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQ-ELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWN-QIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKK----LMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVS-GSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA--------LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITK 10088
            Y L +  P+ + N LP K++Y                 SQI++ ++    L L                 Y   +W     +    ++++ INF       +S +D+ +H+    GQ  V  +SPYWM+NKT   L YKA D    +H    T  VL                           +S Q                        N+  N N       K  L + DS  SD+FS+DTVGS G + CK   + Y+VGV INLSS  +T+IVTF+P+YMI N+S    Y   + E     W+ +   +++PFWP +    S ++  +VE  E    + +   + + IL++L  +  GI  EV + E + +I    Y +G A   LINH   D  + + Q +  + E  LP  + V Y W D    R+  W       + ++    D +    + +   Y VSF     RI+LFT D  V K    S   E  + E+ ++L  +G+SLV+N  +QE+AYI ITSS V W  + ++K R KP+ +  +E LE  F E+     T  + +E+ +V+++ + V L+       +++P    + R + P + ++Y TS HQ    ++IY+IQI NQ+    FP VF P  PPRSV +D+  KP  ++S V+  +G +   R KYF+VLIQEM L LD GFV ++ D V+   V    E   F KD +         S++       +  +F+ FHISPIK+HLS SLS      K +        +HS  LNL L+S+G  +TD+QDV+FKL +FE        +++ S +  HY  QA++QMYVL+LGLDVLGNPFG+IR  ++GVE  FYEP +GA+ GPEEFVEG+A G+K+L G AVGG AGA S+I   M KG+AA+T+D DYQ+KRRE + K+P  L  G+ +GGKGL+ GFV G+TG+VTKPI GA+K+G  GFFKGVGKGLVG VTRP  G++D ASS+F+GI+R  +T+ EV  +RPPRF  +DG+IR Y LR+  GN + +++ E         Y  HV   +K ++  +T + +  + +    G  + +WQ  +++    P I   +  +    KE+ + +F      K
Sbjct: 2096 YVLHLWPPILIRNLLPYKVAYYIEGIENTVVTLSEGHSSQIYNVEMDQAKLHLK-------------LLDYLNHDWKSEFYIRSSQQDINFINFTCLTEMEKSDLDIAIHMTYNTGQTVVAFHSPYWMVNKTNRMLQYKA-DGIHRKHPPNYTKPVL---------------------------FSFQP-----------------------NHFFNNN-------KVQLMVTDSELSDQFSIDTVGSHGAIRCKGLKMEYQVGVTINLSSFNITRIVTFIPFYMIKNKSK---YHISVAEEGSDKWLSLDLEQSIPFWPEN---ASNILLIQVERSEDPPKRIYFNKQDNCILLRLNNELGGIIAEVNLAEHSTVITFSDYHDGAATFLLINHTKSD-PVQYNQSSLGEIEDSLPPGKAVYYTWADPVGSRKLKWSCGQSYGEVTHK--DDMMTPISVGKKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAELEVVLALQDVGISLVNNYTKQEVAYIGITSSDVVWEAKPKKKARWKPMSVKHTEKLEKEFREY-----TEASPLEDKVVELDNIPVRLTPSGNDMKILQPHVIPVRRNYLPALKVEYNTSAHQSSFRIQIYRIQIQNQIHGAIFPFVFYPIKPPRSVTMDSAPKPFTDVSIVMRSAGHSQISRIKYFKVLIQEMDLSLDLGFVYALADLVTKAEVTEKTEVEHFHKDVEAFEQEYEVVSSVDQ----SQVNLFEYFHISPIKLHLSVSLSSGRDEAKDSEQHGGLIPVHS--LNLLLKSIGATLTDVQDVVFKLAFFELNYQFHTTSELQSEVIRHYSKQAIKQMYVLILGLDVLGNPFGLIREFSEGVEAFFYEPYQGAIQGPEEFVEGMALGLKALVGGAVGGLAGAASKITSAMAKGVAAMTMDEDYQQKRREAMNKQPAGLREGITRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVTRPTGGIIDMASSTFQGIKRATETS-EVESLRPPRFFNEDGVIRPYRLRDGSGNQM-LQVMENGR-FAKYKYFTHVMI-NKTDMFMITRRGVLFVTKG-TFGQLTCEWQYTFDEFTKEPFIVHGRR-LRIEAKERVKSVFHAKEFGK 3139          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Mouse
Match: Vps13a (vacuolar protein sorting 13A [Source:MGI Symbol;Acc:MGI:2444304])

HSP 1 Score: 717.227 bits (1850), Expect = 0.000e+0
Identity = 577/2046 (28.20%), Postives = 998/2046 (48.78%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETK-DSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFF-KSVKMDSLAVYWNSNPKPFS-SENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQV--LRAINVLPDSETASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIG--TTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTSV----VLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSN--FNEAINQVPSVPESKEEVKSRKILPKDDKSGSART---------ASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIR-NIKNDSSDFISIKFIQDKNSNSKLIVD--IAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTL--TAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPL---LEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIF---GAPYVVKNTCGKDV 6062
            MVFES+VVE++N++LGDYVV LD+SQL LG+  G   L+NL IKENA  + D+P KV+ GHI  L LKIPWK+LY++PV A L+ +++LI+P+S ++YDP KE   L ETKQ+EL RIE+ K K  D +K  E K D+F EKL  QI++NLQ+ IS+IHIRYED  T  ++  + GI+L N+S QT D      +   D+T +   K +++D+L  YWN N + F  ++   +LKA +KN I +         F+ +PIS SA + ++ + +  +F+ P+++L + L  I   FN+ QY  +   L+S+D       +RK++P+VP+  +   WW +  + ILE  V    RMWSW++I+ HR + + Y + +++KL  KK S     +++  E+ LD+ NIT+ RQQAE++AKK   K+ ++       +  +       S S A+   D     +    T EEK+ LY+AIGYSE   DP +P  + +LK  + L+ +  ++ +N  K  +L+ +V+  +S  V QR  A AI     +++  +TG    D   P L+S+   + L+    + FE NPL+++  Q+  I AEPLEIIYD+ TVN IV+F  PP+++ L ++++  L  +EE +  TATGL ++ + +   D+++++K SY I+P+ G ++    +LL+DLG + + S+R S+       L   +  + + + +   AYD F I L++IQ L    GD+W++ R    S  HIL P      L K ++  D  +PK  + G LP + + ISD+ LQ ++EL+  IP      P+PV    +  R +   Q    P  +++   +  L       DSE     +            ++  ++   +    +KK  K   + N+I L   F +    +Q    +        G  E+  L +  +G  T    + FD+   A++  L L  P+++D   K PV L  T  +T  ++LL +  + VEK +P+  + + N    ++  F S+++ LH EALL  +N++N+++P L     EK+ + S+   E+                    K D  I+  +  ++    +  T++L AE            S  +IFI                       ++  NI +I I G+ +++      TE++  + ++ + D   +A+YKK ++I   E +FS ++  +   T+   Y  D+S VD  V++ VG I+++F+  F+  I  F+++FQ     +A    + +E AA+ +  +  +  SSR  LD+N+KAPV+ IP++  S N  + D G +T KN F     T      V++   I LS +R+ RS    +T +    +L P+N+ + + RNLSW     +P  +I A++  +   +SQ D       L  N  + + ++   S  +  E + S    P D    +  T         AS     +   F+ ++L + L+    +   +     P   L + K EN+     ++ + +   S  + N  + D +    K    +I   +  D  D + IK+ + K   + ++ D  +  +Y+C  V++LM +   F  A                      R  R+ P        L  +E+++ +  PEI+ V DM   D+ A+++ T   +       S+ +   I D Q   CPF    RK     ++ P  +     +   +     ++++ L + ++P +I +I  I + L  T ++  E +T +           + + W+K++    +  F+ +   E EK+   N+ +  G                                + LR+  I ++LE+ +  + VPML+ +    G+ KN L+ I L   + + V Y+N+   VWEPL   LE+   +  R ++  I+        + + +   +   +     +I   SRD L +T+SK  L +L+NL +AF +A +       S+F    AP+++ N+ G  V
Sbjct:    1 MVFESVVVEVLNRFLGDYVVNLDESQLSLGIWKGAVALKNLVIKENALHELDVPFKVKVGHIGSLKLKIPWKNLYTQPVEAVLEEIFLLIVPSSRIQYDPIKEEKQLMETKQQELKRIEKAKQKVFDKEKPREEKQDTFTEKLVTQIIQNLQVQISSIHIRYEDDITNGDKPLSFGISLQNISLQTTDQYWIPCL--HDNTEKLVRKLIRLDNLFAYWNVNSEMFYLNDYDESLKA-LKNGIVNENIVPEGYDFVFRPISASAKLQMNRRSD-FDFSDPKINLAVDLHTIAIEFNKPQYFSLMELLESIDMMTQNQPYRKFKPSVPLHLHAKEWWAYAIHSILEVNVCPSLRMWSWEHIRNHRYKMKRYREFYKKKLTSKKPSPEILMSLEELEKTLDVFNITIARQQAEVEAKKAGYKIYKEGVKDPEDNAGWFGWLWTWSESNANQQQDVKPGILEEMLTPEEKSLLYEAIGYSETAVDPTLPKTFEALKFFVHLKSMSIVLRENHQKP-ELLNVVVEGLSTSVVQRPGAQAIKFETKIDSFHITGLPD-DFKKPHLLSSLDDTSLLQ---ITFEINPLNETVAQRCTIEAEPLEIIYDARTVNSIVEFFRPPKDVHLAQLTSVTLTKLEEFRAKTATGLLYVIETQKVLDLRINVKASYVIVPQYGNFSPTSNLLLLDLGHLKVSSKRRSL-------LPDVRPSEASLEDIMHRAYDSFDIQLTSIQLLYSRVGDNWKEARKLNVSTQHILIPMHVNVELSKAMVFMDIKMPKFKISGKLPLVSLRISDKKLQGIMELLGSIP-----KPEPVTDVSAPARSFQ-IQASALPVSHISQKLIPLLEQPVTEDDSEEEFFDAPC-------SPLEECPQVSCRDKCTRQKKLQKKDCVMNLIQLRMRFEVAEVSIQFYHLV--------GDCELPVLEMGALGLGTEAEFRTFDLKGSAFLKELWLKCPEYLD-ENKKPVYLITTLDNTM-EDLLTLEFMKVEKNAPNLNSTYNNVLQLIKVNFSSLDIHLHTEALLNTMNYLNNILPEL----REKSASVSAAEPED--------------------KGD--IIKKLALKLPTNEDIITLQLLAEL-----------SCLQIFI----------------------QDQKQNISEIKIEGLDSEMIMKPLVTEINAKLRNIIVLDSDKMAIYKKALYITGKE-VFSFKMISYMDATAGYAYT-DMSVVDIRVHLTVGCIEVVFITKFLYSILAFIDNFQAVKDALAEATVQAAEMAADGVKELARK--SSRFALDVNIKAPVVLIPQSPVSQNVFVADFGLITMKNIFVTVTETQSNIPPVIDLITIKLSKMRLYRSQFRNDTYQEVLDLLLPLNLEVIVERNLSWEWYKEVPCFNIKAQLKPMEFILSQEDLTTVFQTLHGNIWYGQDLSAPSSANKDPETMTSGVTSPPDHSPATVVTAAVVEVHPQASQAHTMLNVSFQTDYLTMALYSPGPDEASFTDVRDPSLELAEFKLENIISSLKIYTDDSTVFSFSVKNCILDDKRSHVMKATPRMIGLTVGFDKKDMVDIKYRKIK---TFVVTDAVVQEMYVCASVEFLMTVAHIFFDAYMTSTALETSVQT---------RTTREAPAQ-----ELGKWEMNILIKNPEIVFVADMTRNDAPALVITTQCEICCKGEPTSNTVTAAIKDLQVRACPFLPVKRKGKVTTVLQPCDLFYQATQLGRDPQMIDISVKSLTLKVSPVIINTIITITSALYTTKETVPEENTSN-----------IAHLWDKKDTKNLKMWFLEES-NESEKVVPTNEVMPGG------------------------------ETLNLRIDSIFIVLEAGIGHRTVPMLLAKACFSGESKNWLSLINLHCHLELEVHYYNEMFGVWEPLLEPLEIDQTDDFRPWNLGIKMKKKAKEAIVESDSEAENYKVPEYKTAISFYSRDQLNITLSKCGLVMLNNLVEAFTEAAT----GSSSVFLRDLAPFMIFNSLGLTV 1881          

HSP 2 Score: 103.605 bits (257), Expect = 5.952e-21
Identity = 82/290 (28.28%), Postives = 118/290 (40.69%), Query Frame = 3
Query: 6765 YKLDINVPLALHNQLPIKISY----------------QSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQSLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQS 7586
            Y L +  P+ + N LP K++Y                 SQI++ ++    L L                 Y   +W     +    ++++ INF       +S +D+ +H+    GQ  V  +SPYWM+NKT   L YKA D    +H    T  VL                           +S Q                        N+  N N       K  L + DS  SD+FS+DTVGS G + CK   + Y+VGV INLSS  +T+IVTF+P+YMI N+S
Sbjct: 2096 YVLHLWPPILIRNLLPYKVAYYIEGIENTVVTLSEGHSSQIYNVEMDQAKLHLK-------------LLDYLNHDWKSEFYIRSSQQDINFINFTCLTEMEKSDLDIAIHMTYNTGQTVVAFHSPYWMVNKTNRMLQYKA-DGIHRKHPPNYTKPVL---------------------------FSFQP-----------------------NHFFNNN-------KVQLMVTDSELSDQFSIDTVGSHGAIRCKGLKMEYQVGVTINLSSFNITRIVTFIPFYMIKNKS 2314          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Mouse
Match: Vps13c (vacuolar protein sorting 13C [Source:MGI Symbol;Acc:MGI:2444207])

HSP 1 Score: 640.188 bits (1650), Expect = 0.000e+0
Identity = 483/1672 (28.89%), Postives = 819/1672 (48.98%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEE--------------------------------------------NKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQ-TAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSE--TASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQV----PSVPESKEEVKSRKI-LPKDDKSGSARTASD-------ESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIK-NDSSDFISIKFIQDK-------------NSNSKLIVDIAGIYMCICVDYLMMLTDFF 4808
            MV ES+V +L+N++LGDYV  L+KSQLKLG+ GGN  L+NL IKENA  + D+P KV+ G I KLTLKIPWK+LY E V+A L+GLY+L++P + +KYD EKE   L + KQKEL RIEE                                              L R+  K +   KD+F EKLA Q++KN+Q+ I++IHI+YED  T P R  + G+TL   S  T +   T  I+ E + +  +K VK+DSL+ YWN         +R  +   +K  I +S     D  ++ QPIS SA ++++   E+     P++  +++++ I     + QY  +  FL+S+D       +RKY+P +P+ +N   WW +  + +LE  +RR  + WSW  I+ HR   + Y   ++ KL   K+S+  Q  I+  E+ LD+ NI L RQQA+++     +KL +K++  G   GWF     +  S    D    + + +    T EEK KL+ AIGYSE   +  +P +YV+  L L+L     II +N     +++++ I  +   V QR  A A+ +   + +  VTG +  D  VP+LV++   +   +LL ++FETNP +  A+Q + ++++P+E+IYD+ T+N +V+F    + L L +I++  L  +EE+KE TATGL H+ + R   D+++++KPSY I+P+ G + +   +L++D G+  + S+      + +  L          + + + AYDKF + + ++Q L  +  ++W+K R Q  S  HIL+P      L K ++  D  + K  V G LP + V ISD+ ++  L LI  IP PQ ++ P P     SI        L    K  L ++ +L  +    D E   A    +      +  E K A E+ +                  ++ L   F I    L++ +  K         + I+   V ++GT  T++ FD+   +Y+  + L     +  S K P+ L  +     G +LL +  + V++  P F T    T   ++  F S+ + L  +ALL  LN++ ++IP                      + T + KE +A+ + ++      +M                       S  SD  G+    ++F            A L    V V +EK +NI +I I G+ + +        +   + ++ + D  P  ++KK + I  +E +F   + L+   T    Y  D+S VD +V + VG I+I++L+ F++ + +FLN+FQ + + ++    + +E AA  + ++ +   S R  +DI++KAPVI IP++S S NA++VDLG +   N F++    D +   V+++ ++ L+ L++SR+ +   T   +  +LHPIN+   ++RNL+ N  H +P + I  R+ S++V ++Q D N+   +L  N  EA   +    P + E  E    R++  P+D  +     A+        + I V   F+++ + V L K          E         +K  +L +E  +  +  M  +  L NI++       +K   E +R +  +D SD I +K +  K             N+  KL V  + + + + ++ L+ L DF 
Sbjct:    1 MVLESVVADLLNRFLGDYVENLNKSQLKLGIWGGNVALDNLQIKENALSELDVPFKVKAGQIDKLTLKIPWKNLYGEAVVATLEGLYLLVVPGASIKYDAEKEEKSLQDIKQKELCRIEEALQKAAEKGAHSGEFMYGLENLLYKDVKPGRKRKKHKKHFKKRFKGLDRSKDKPKEAKKDTFLEKLATQVIKNVQVKITDIHIKYEDDITDPERPLSFGVTLREFSLLTTNEHWTPCILNE-AEKIIYKLVKLDSLSAYWNVGCCMSYRGSREHILEQLKREILTSTNIPPDHQYIFQPISASAKLYMNPGAES-ELKTPKLDGNVEVQNIAIELTKPQYLSMIDFLESLDYMVRNAPYRKYKPCLPLHTNCRQWWKYAIDSVLEVHIRRYTQPWSWSNIKNHRQLLKSYKMAYKTKLTQAKVSEEIQKQIQDLEKSLDVFNIILVRQQAQVEVIHSGQKLRKKSAEAGEKRGWFSGFWGKKESK-KRDEESSVPETIDDLMTPEEKDKLFTAIGYSENAYNLALPKQYVAHILTLKLVSTSIIIRENR-NVPEILRVQIIGLGTQVSQRPGAQALKIEAKLEHWYVTGLRQQD-IVPSLVASIGDTT-SSLLKIEFETNPENSPADQTLIVQSQPVEVIYDAKTINAVVEFFQSNKGLDLEQITSATLMKLEEIKERTATGLTHIIETRKVLDLRINLKPSYLIIPQTGFHHEKSNLLILDFGTFQLNSKDQGAQKTANASL----------EEIIDKAYDKFDVEIRSVQLLFAKAEENWKKCRFQHPSTMHILQPMDIHVELAKAMVEKDVRMAKFKVSGGLPLMHVRISDQKIKDALCLINSIPLPQKSSTPSPERQVASI------PVLSGGTKALLGTSLLLDGVESESDEEFFDAEDGDSQAARTVKASELKKAAEVPNE----------------ELVSLLLKFEIKEVVLELTKQQKE-------EETILVFNVTQLGTEATMRTFDLTAVSYLRKISLDY-HDIKGSRKKPIHLISSSDRP-GLDLLKVEYIKVDRNGPSFQTTFEKTEQTVKVAFSSLNLLLQTQALLSSLNYLTTVIP-------------------SDSQNTGVAKEVQAMPEKQKNSPLQKVM---------------------VPSRDSDVIGF----RLF------------AKLNAFCVTVCDEK-SNIAEIKIQGLDSSLSLQSKKQSLFARLENIIVTDVDPKTIHKKAVSIVGNE-VFRFNLDLYPDATEGDSYT-DMSTVDGVVALHVGCIQIVYLHKFLMSLLSFLNNFQVAKEALSAATAQAAEKAATSVKDLAQR--SFRVSVDIDLKAPVIVIPQSSLSTNAVVVDLGLIRVHNRFSLVSGEDTANPPVIDKMEVQLTKLKLSRTAIQPGTSHPDIQLLHPINLEFFVSRNLAANWYHKVPVVEIKGRLDSMNVSLNQEDLNLLFRILAENLGEATEDLDKGKPRIQERGETKACREVSTPQDVHTTQGVPAARVEETRPVDIINVLLNFEIKEVVVTLMKKAERKGSPFHE---------LKILHLGMEAKVKAHD-MTAAAYLRNISMRCFHFPDSK--GEPLRIVNTSDVSDGILLKLLFIKADSDGPDFKTIHDNTKQKLKVSFSSLDLVLHLEALLSLMDFL 1552          

HSP 2 Score: 557.755 bits (1436), Expect = 1.296e-158
Identity = 390/950 (41.05%), Postives = 573/950 (60.32%), Query Frame = 3
Query: 7368 KNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG-----WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFY--ESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDF-YINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEG-LEVDLSKKLMIK--PEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEA------MFVKDCQMIRASLIETS-TIKTILSGGKKCVFDNFHISPIKIHLSFSL------SRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKS-KKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINES------KHTIVFTLKEKQ-----RKLFSKDNITKEIEADLI 10109
            K   F+K  K  LKI+ S WS+ FSLDTVGS G V C    + Y VGV I +SS  L+++VT  P+  + N+SS+ L   E+  +       W  V   E +PFWP +  GK  +     E      ++PF FY  + +  L+ LE    GI V++   E + +I    Y EG A   ++NH   DV + +KQ  + +EL+L   +T ++AW D    R+  W    +  +H  +L  D    F Y   ++ +WVSF+    R+LLFT+D+ +   A  + ++E  D E+ +SLHS+GLSLV+N  +QE++Y+ ITSSGV W +++ K + KP    Q  +LE A+ +   RL    A  +   VK++   EV+  K  M    P +C + R F  G+ ++++ SPHQ  +  R+Y +Q+DNQ+    FPVVF P  PP+S+ALD+  KP I++S +   +  +  +++KYF VLIQEM L +DQGF+ +++   +   PT D EA      +  +D   +   L+E+S T  +ILS      F++FHISP+K+HLS SL      S   +    A+HS  +NL L+S+G  +TD+ D+IFKL Y+E +      +Q++ S+  HY  Q L+QMYVLVLGLDVLGNPFG+IRG+++GVE LFYEP +GAV GPEEF EG+  GV+SL GH VGGAAG VSRI G++GKG+AA+T+D +YQ+KRRE++ ++P++ G  LA+GGKG + G V GVTG++TKP++GAKK+G  GFFKG+GKGLVG V RP  G++D ASS+F+GI+RVA++  EV  +RPPR I +DGIIR Y  +E+EG+++     +K +    E Y FH      K+ V+ +TN+R   +   EILG  S+ WQ  +E    PP ++E+      K   +F  K+       RK++ KD IT +   D I
Sbjct: 2802 KKNIFSKN-KVQLKISTSAWSNGFSLDTVGSYGCVKCPATNMEYLVGVSIKMSSFNLSRVVTLTPFCTVANKSSLDLEVGEIASDGSIPTNKWHYVASSECIPFWPENLSGKLCVRVVGYEGS----SKPF-FYNRQDNGTLLSLEDLNGGILVDINTAEHSTVITFSDYHEGSAPALIMNHTQWDV-LTYKQSGSQEELVLLPGETRLFAWADPTGIRKLTWNYAANFGEH--DLLKDECGQFPYDANIQIHWVSFLDGRQRVLLFTDDVALVSKALQAEEMEQADHEVALSLHSLGLSLVNNENKQEVSYVGITSSGVVW-EMKPKQKWKPFSQKQIMSLEQAYSK---RL----ASQDRGWVKLDSNFEVNFDKVPMEMRLPIRCPIKRDFLSGIQVEFKQSPHQRSLRARLYWLQVDNQLPGTMFPVVFHPVAPPKSIALDSEPKPFIDVSVITRFNEYSKVLQFKYFMVLIQEMALKVDQGFLGAVISLFT---PTTDPEAERKRTKLIQQDIDALNTELMESSMTDMSILS-----FFEHFHISPVKLHLSLSLGSGGEESDKEKQEMIAIHS--VNLLLKSIGATLTDVDDLIFKLAYYEIRYQFYKRDQLMWSVVRHYSEQFLKQMYVLVLGLDVLGNPFGLIRGLSEGVEALFYEPFQGAVQGPEEFAEGLVIGVRSLVGHTVGGAAGVVSRITGSVGKGLAAITMDKEYQQKRREEMGRQPKDFGDSLARGGKGFLRGVVGGVTGIITKPVEGAKKEGAAGFFKGIGKGLVGAVARPTGGIIDMASSTFQGIQRVAESTEEVSSLRPPRLIHEDGIIRPYDRQESEGSDLLENHIKKLE---GEAYQFHCAVPGNKRAVLMITNRRALFIKEVEILGHMSVDWQCLFEDFVCPPEVSENLLKISVKEQGLFHKKDSANIGHLRKIYLKDPITAKRAFDAI 3721          

HSP 3 Score: 341.273 bits (874), Expect = 7.369e-93
Identity = 352/1494 (23.56%), Postives = 663/1494 (44.38%), Query Frame = 3
Query: 2826 IVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTS--PDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTSVVL----EESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSG----------SARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELI--RNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXM-VPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFE--IEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDA------YSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADS--ISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKC---YNVPIPTLLTSNGKIYIS-VKQLNTEISNSGAL--WNIGKNAQPSDTQILLCKS---NTGVMYYYSVALSIEEVQIVNSSETLR-TYKLDINVPLALHNQLPIKISY--QSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAV 7184
            I+H +G    +K  DM   AY+ ++ +    F D S   P+ +  T   + G   + + LL ++  S  PDF T+H NT+ KL+  F S+++ LH EALL +++F++S                       +   ++ EKE E    LK                 L  E R++ ++A   S + D   +D                ITA L    + + ++K +NI +I IHGM A +      T+V   + ++ + +   L+++KK + I  DE +F  Q++L+   T    Y  D+S+VD  ++++VG I+I++++ F + + +FLN+FQ + + ++    + +E AA+ + ++ ++  S R  +DI++KAPVI IP++S S N ++ DLG +  +N F++     + L    +E  I L+ L+++R++L  ++ +++  IL P+N+ + + RNLS      IP + I   +  + V +SQ D  +   +L  N  EA +Q PS  +  +E    K   +D +SG            +   D+++T+QF F  + L + L+  D++     S       L ++    ++  G M  + +M VS++L    + D +    +  + +I  +N ++++S  I I + QDKN  S++   +  +Y+C  V++LM + DFF+KA P   +   K + +P R       K  K     P      +  +   +  PE++ V  +   D+ A+       L  S  K   ++   + D + + CPF  E R K+   ++ P S+ +      +     ++ ++  +I I+P ++ ++  IM  ++ +      T++  + +   +   DN W  +   +    F+               GV+   +V +   D E                  E    + +  ++V LE  +  + VP+L+ E    G++KN  + +     +++ V Y+N+   VWEPL+E    E N+ +S +  ++     D  L   +DF     I     ++ + S   + +T+SKS L+V SNL K F +       YSL          AP+ VKN  G  V   +    +LK   S E+  +Y    G+    D    + S     +Q K+      +S   +  ++    TEV ++  +       +  N S    ++++V   A  G K + LRS LQ+ N+      +Y            KN  LLE +   +    ++VP+ +      ++Y+     L  + ++S     W    +       +L C +   +   +   +VAL  E   I    E     Y + +  PL L N LP  + Y  +    +H++  G    V  + +  +    V   Y  K W G   +   + E  ++ F +      + +DL VH+     +  ++++SPYW++NKT   L Y++ ++  V+H A   D +
Sbjct: 1452 ILH-LGMEAKVKAHDMTAAAYLRNISMRCFHFPD-SKGEPLRIVNTSDVSDG---ILLKLLFIKADSDGPDFKTIHDNTKQKLKVSFSSLDLVLHLEALLSLMDFLSS--------------------AIPSSDSSSSEKEPE----LK----------------PLVGESRSLAIRAVPSSYEGD--AFD--------------LKITAELNAFNIFICDQK-SNIAEIKIHGMDASISVKPKQTDVFARLKNIIVMNVDSLSIHKKAVSILGDE-VFRFQMSLYPDATEGENYG-DMSKVDGRLSLKVGCIQIVYVHKFFMSLLSFLNNFQAAKEALSTATVQAAERAASSVKDLAQK--SFRLLMDIDLKAPVITIPQSSVSPNVVIADLGLIRVENKFSLVSVEQLALPPVADEMSIQLTQLKLARTVLQADSPQHDVEILKPVNMLLCIQRNLSAAWYTQIPGMEIKGELKPMQVALSQDDLTVLMKILLENLGEASSQ-PSPTQYAQEAARVK---RDTRSGPDYLKEQELADPKPPGDQTVTLQFDFHFDSLSIILYNSDSSQEPRLSFHNDSFRLGELTLHLMASAGKMFKDGSMNVSLKLKTCTLDDLREGIERATSRMIDKKNDQDNNSSMIDISYSQDKN-GSQVDAVLDKLYVCASVEFLMTVADFFIKAMPQSPENIAKEIQIPSRQTAAGRVKMEKDDSVRP------NMTLKAMITDPEVVFVASLTKADAPALTASFQCNLSLSTSKLEQMMEASVRDLKVLACPFLRERRGKSITTVLQPCSLFMEKCTWASGKQNINIVVKEFVIKISPIILNTVMTIMAAMSPK------TKEDEWKDTPKE--TDNLWAVKSITDYNSWFL---------------GVDMATEVTENFRDSEHPSI--------------EENCVVAVESVQVTLECGLGHRTVPLLLAESKFSGNIKNWTSLMAAAADMTLEVHYYNETHAVWEPLIERV--EGNKPWSLKLNVKKNPIQDKSLMPGDDF-----IPEPQTAVHISSGATMNITISKSCLNVFSNLAKGFSEGAASTFDYSLKDR-------APFTVKNALG--VPMKVQPNRNLKVMGSPEKSDIYDVGAGQHLELDYASLEPS-----RQGKLSILSRQESSLFTLTFVPYGYTEVASVPVARPGRRLYNVRNPSASHSDSVLVQIDATEGNKVVTLRSPLQIKNHFSIAFIIYKF---------VKNVKLLERIGIARPEEEFHVPLDSY---RCQLYVQPAGGLEQQYTHSSTYISWKEELHRSREVRCMLQCPAVEVSFLPLIVNTVALPDELSYIGAHGEDWDPAYVIHLYPPLTLRNLLPYSLRYLLEGTAETHELAEGSSADVLHSRISGEIIELVLVKYLGKNWNGHFRICDTLPEFFLVCFSSDTAEVMT-VDLSVHVRRIGCRMELSVFSPYWLINKTSRVLQYRS-EEIHVKHPADFRDII 2796          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Mouse
Match: Vps13a (vacuolar protein sorting 13A [Source:MGI Symbol;Acc:MGI:2444304])

HSP 1 Score: 504.597 bits (1298), Expect = 2.902e-154
Identity = 364/925 (39.35%), Postives = 518/925 (56.00%), Query Frame = 3
Query: 7086 SPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQSLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQ-ELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWN-QIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKK----LMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVS-GSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA--------LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNI 9815
            SPYWM+NKT   L YKA D    +H    T  VL                           +S Q                        N+  N N       K  L + DS  SD+FS+DTVGS G + CK   + Y+VGV INLSS  +T+IVTF+P+YMI N+S   +   E   +  W+ +   +++PFWP +    S ++  +VE  E    + +   + + IL++L  +  GI  EV + E + +I    Y +G A   LINH   D  + + Q +  + E  LP  + V Y W D    R+  W       + ++    D +    + +   Y VSF     RI+LFT D  V K    S   E  + E+ ++L  +G+SLV+N  +QE+AYI ITSS V W  + ++K R KP+ +  +E LE  F E+     T  + +E+ +V+++ + V L+       +++P    + R + P + ++Y TS HQ    ++IY+IQI NQ+    FP VF P  PPRSV +D+  KP  ++S V+  +G +   R KYF+VLIQEM L LD GFV ++ D V+   V    E   F KD +         S++       +  +F+ FHISPIK+HLS SLS      K +        +HS  LNL L+S+G  +TD+QDV+FKL +FE        +++ S +  HY  QA++QMYVL+LGLDVLGNPFG+IR  ++GVE  FYEP +GA+ GPEEFVEG+A G+K+L G AVGG AGA S+I   M KG+AA+T+D DYQ+KRRE + K+P  L  G+ +GGKGL+ GFV G+TG+VTKPI GA+K+G  GFFKGVGKGLVG VTRP  G++D ASS+F+GI+R  +T+ EV  +RPPRF  +DG+IR Y LR+  GN +
Sbjct:    2 SPYWMVNKTNRMLQYKA-DGIHRKHPPNYTKPVL---------------------------FSFQP-----------------------NHFFNNN-------KVQLMVTDSELSDQFSIDTVGSHGAIRCKGLKMEYQVGVTINLSSFNITRIVTFIPFYMIKNKSKYHISVAEEGSDK-WLSLDLEQSIPFWPEN---ASNILLIQVERSEDPPKRIYFNKQDNCILLRLNNELGGIIAEVNLAEHSTVITFSDYHDGAATFLLINHTKSD-PVQYNQSSLGEIEDSLPPGKAVYYTWADPVGSRKLKWSCGQSYGEVTHK--DDMMTPISVGKKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAELEVVLALQDVGISLVNNYTKQEVAYIGITSSDVVWEAKPKKKARWKPMSVKHTEKLEKEFREY-----TEASPLEDKVVELDNIPVRLTPSGNDMKILQPHVIPVRRNYLPALKVEYNTSAHQSSFRIQIYRIQIQNQIHGAIFPFVFYPIKPPRSVTMDSAPKPFTDVSIVMRSAGHSQISRIKYFKVLIQEMDLSLDLGFVYALADLVTKAEVTEKTEVEHFHKDVEAFEQEYEVVSSVDQ----SQVNLFEYFHISPIKLHLSVSLSSGRDEAKDSEQHGGLIPVHS--LNLLLKSIGATLTDVQDVVFKLAFFELNYQFHTTSELQSEVIRHYSKQAIKQMYVLILGLDVLGNPFGLIREFSEGVEAFFYEPYQGAIQGPEEFVEGMALGLKALVGGAVGGLAGAASKITSAMAKGVAAMTMDEDYQQKRREAMNKQPAGLREGITRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVTRPTGGIIDMASSTFQGIKRATETS-EVESLRPPRFFNEDGVIRPYRLRDGSGNQM 849          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Mouse
Match: Vps13c (vacuolar protein sorting 13C [Source:MGI Symbol;Acc:MGI:2444207])

HSP 1 Score: 461.455 bits (1186), Expect = 1.221e-140
Identity = 326/772 (42.23%), Postives = 483/772 (62.56%), Query Frame = 3
Query: 7572 ILNQSSIVLYCNELVENAG-----WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFY--ESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDF-YINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEG-LEVDLSKKLMIK--PEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEA------MFVKDCQMIRASLIETS-TIKTILSGGKKCVFDNFHISPIKIHLSFSL------SRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNI 9815
            + N+SS+ L   E+  +       W  V   E +PFWP +  GK  +     E      ++PF FY  + +  L+ LE    GI V++   E + +I    Y EG A   ++NH   DV + +KQ  + +EL+L   +T ++AW D    R+  W    +  +H  +L  D    F Y   ++ +WVSF+    R+LLFT+D+ +   A  + ++E  D E+ +SLHS+GLSLV+N  +QE++Y+ ITSSGV W +++ K + KP    Q  +LE A+ +   RL    A  +   VK++   EV+  K  M    P +C + R F  G+ ++++ SPHQ  +  R+Y +Q+DNQ+    FPVVF P  PP+S+ALD+  KP I++S +   +  +  +++KYF VLIQEM L +DQGF+ +++   +   PT D EA      +  +D   +   L+E+S T  +ILS      F++FHISP+K+HLS SL      S   +    A+HS  +NL L+S+G  +TD+ D+IFKL Y+E +      +Q++ S+  HY  Q L+QMYVLVLGLDVLGNPFG+IRG+++GVE LFYEP +GAV GPEEF EG+  GV+SL GH VGGAAG VSRI G++GKG+AA+T+D +YQ+KRRE++ ++P++ G  LA+GGKG + G V GVTG++TKP++GAKK+G  GFFKG+GKGLVG V RP  G++D ASS+F+GI+RVA++  EV  +RPPR I +DGIIR Y  +E+EG+++
Sbjct:    3 VANKSSLDLEVGEIASDGSIPTNKWHYVASSECIPFWPENLSGKLCVRVVGYEGS----SKPF-FYNRQDNGTLLSLEDLNGGILVDINTAEHSTVITFSDYHEGSAPALIMNHTQWDV-LTYKQSGSQEELVLLPGETRLFAWADPTGIRKLTWNYAANFGEH--DLLKDECGQFPYDANIQIHWVSFLDGRQRVLLFTDDVALVSKALQAEEMEQADHEVALSLHSLGLSLVNNENKQEVSYVGITSSGVVW-EMKPKQKWKPFSQKQIMSLEQAYSK---RL----ASQDRGWVKLDSNFEVNFDKVPMEMRLPIRCPIKRDFLSGIQVEFKQSPHQRSLRARLYWLQVDNQLPGTMFPVVFHPVAPPKSIALDSEPKPFIDVSVITRFNEYSKVLQFKYFMVLIQEMALKVDQGFLGAVISLFT---PTTDPEAERKRTKLIQQDIDALNTELMESSMTDMSILS-----FFEHFHISPVKLHLSLSLGSGGEESDKEKQEMIAIHS--VNLLLKSIGATLTDVDDLIFKLAYYEIRYQFYKRDQLMWSVVRHYSEQFLKQMYVLVLGLDVLGNPFGLIRGLSEGVEALFYEPFQGAVQGPEEFAEGLVIGVRSLVGHTVGGAAGVVSRITGSVGKGLAAITMDKEYQQKRREEMGRQPKDFGDSLARGGKGFLRGVVGGVTGIITKPVEGAKKEGAAGFFKGIGKGLVGAVARPTGGIIDMASSTFQGIQRVAESTEEVSSLRPPRLIHEDGIIRPYDRQESEGSDL 748          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. UniProt/SwissProt
Match: sp|Q96RL7|VP13A_HUMAN (Vacuolar protein sorting-associated protein 13A OS=Homo sapiens OX=9606 GN=VPS13A PE=1 SV=2)

HSP 1 Score: 1227.23 bits (3174), Expect = 0.000e+0
Identity = 1026/3439 (29.83%), Postives = 1665/3439 (48.42%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKE-TETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFF-KSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSS-------SLASDGSDELQKKVRSE-----FTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGS-VPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSE-RTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQ-----TAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSN--FNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDE-----------SITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELI--RNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLE---VYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFS--PKDNEGPSRGNVSEREIN---TLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPT-----LLTSNGKIYISVKQLNTE--ISNSGALWNIG-KNAQPSDTQILLC----KSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISY-----QSQIFSHDIKPGCLELVRTACL-KVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQSLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFY---ESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQ-ELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSN-NLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKW-NQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVE-GLEVDLSKK----LMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEA-MFVKDCQMIR-----ASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA--------LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLF 10067
            MVFES+VV+++N++LGDYVV LD SQL LG+  G   L+NL IKENA    D+P KV+ GHI  L L IPWK+LY++PV A L+ +Y+LI+P+S +KYDP KE   L E KQ+EL RIEE K K  D ++   E +D+F EKL  QI+KNLQ+ IS+IHIRYED  T  ++  + GI+L NLS QT D      +   D T +   K +++D+L  YWN   + F   +       +KN I +         F+ +PIS +A + ++ + +  +F+ P+++L+++L  I   FN+ QY  I   L+S+D       +RK++P+VP+  +   WW +  + +LE  V  R  MWSWK+I+ HR + + Y +++++KL  KK       +++  E+ LD+ NIT+ RQ AE++ KK   K+ ++              D E +        S +   ++E Q  V+ E      T EEKA LY+AIGYSE   DP +   + +LK  + L+ +  ++ +N  K  +L+ +VI   S  + QR  A AI     +++  +TG   PD S  P L+S+   +  M+L  + FE NPLD++ +Q+  I AEPLEIIYD+ TVN IV+F  PP+E+ L +++   L  +EE +  TATGL ++ + +   D+K+++K SY I+P+ G ++    +LL+DLG + + S+ R+ +P          K G+ N   + + AYD F I L+++Q L    GD+WR+ R    S  HIL P  F   L K ++  D  +PK  + G LP + + ISD+ LQ ++ELI  IP P+     +AP K      S     S     I P L L S     +     D+  + L   +             L+  D +V              NM      F +    ++    + +  +       +V+++V  +G  + ++ +D+   A++    L  P+++D   K PV L  T  +T  ++LL +  +  EK  PD  + + N    ++  F S+++ LH EALL  +N++++++P      EEK+   S+         T  E + + I+ L                + L+T +  + L+                              I A L  L++ + ++K  NI +I I G+ +++      TE++  + ++ + D    A+YKK ++I   E +FS ++  +   T+   Y  D++ VD  VN+ VG I+++F+  F+  I  F+++FQ + Q +A    + +  AA  +  + +   SSR  LDIN+KAPV+ IP++  S N  + D G +T  N F +      S   V++   I LS +R+ RS  + +  +    +L P+N+ + + RNL W     +P  ++ A++  +   +SQ D       L  N  + +  +  P+V + +    S         SG A   +               T+   FK + L + L+        +     P   L + K EN+     M+ + +   S  L N  +LD +    KK    +    +  D  D + IK+ + ++      V    +Y+C  V++L  + + F++A                  E + + +  K      E     +EI+V +  PEI+ V DM   D+ A+++ T   + Y    E+  +   I D Q   CPF    RK     ++ P  +     +   +     ++++ L + ++P +I ++  I + L   +T ET       I  +      + WEK++    +  F+ +   E EKI    + V  G                                I + +  I ++LE+ +  + VPML+ +    G+ KN  + I L   + + V Y+N+   VWEPLLE   +   E  R ++  I+        + + +  E+   +      I   S+D L +T+SK  L +L+NL KAF +A +           AP+++ N+ G  +    +  F +         K Y+  +GE    D +  K +            F    S+S +   I  T V        P    G     V  RE     +++       G K++ +RS +Q+ N+    +++Y            +   LL        +N+P+ +      L    + Y   + ++ E  I N GAL     ++  PS    L+     K N   +  YS                   Y + +  P+ L N LP KI+Y     ++ +F+  +  G    + TA L K + ++K+   Y   +W     + P  +++S ++F       ++ +D+ VH+    GQ  V  +SPYWM+N        K G   Q + D                           I +K    Y    L + +                  N+  N N       K  L + DS  S++FS+DTVGS G V CK   + Y+VGV I+LSS  +T+IVTF P+YMI N+S   +   E   N  W+ +   + +PFWP     K ++   + ED       P   Y   + + IL++L+ +  GI  EV + E + +I    Y +G A   LINH  +++ + + Q +  + E  LP  + V Y W D    R   WR    +K H       D +    + E   Y VSF     RI+LFT D  V K    S   E  +QEI ++L  +G+SLV+N  +QE+AYI ITSS V W  + ++K R KP+ +  +E LE  F E+     T  +  E+ +++++  + V L+       +++P    L R + P + ++Y TS HQ    ++IY+IQI NQ+    FP VF P  PP+SV +D+  KP  ++S V+  +G +   R KYF+VLIQEM L LD GF+ ++ D ++ +  T + E  +F KD +  +     ASL++ S +          +++ FHISPIK+HLS SLS      K +        +HS  LNL L+S+G  +TD+QDV+FKL +FE        + + S +  HY  QA++QMYVL+LGLDVLGNPFG+IR  ++GVE  FYEP +GA+ GPEEFVEG+A G+K+L G AVGG AGA S+I G M KG+AA+T+D DYQ+KRRE + K+P     G+ +GGKGL+ GFV G+TG+VTKPI GA+K G  GFFKGVGKGLVG V RP  G++D ASS+F+GI+R  +T+ EV  +RPPRF  +DG+IR Y LR+  GN + +++ E         Y  HV   +K +++ +T + +  + +    G  + +WQ ++++    P I   +  +    KE+ + +F
Sbjct:    1 MVFESVVVDVLNRFLGDYVVDLDTSQLSLGIWKGAVALKNLQIKENALSQLDVPFKVKVGHIGNLKLIIPWKNLYTQPVEAVLEEIYLLIVPSSRIKYDPLKEEKQLMEAKQQELKRIEEAKQKVVDQEQHLPEKQDTFAEKLVTQIIKNLQVKISSIHIRYEDDITNRDKPLSFGISLQNLSMQTTDQYWVPCL--HDETEKLVRKLIRLDNLFAYWNVKSQMFYLSDYDNSLDDLKNGIVNENIVPEGYDFVFRPISANAKLVMNRRSDF-DFSAPKINLEIELHNIAIEFNKPQYFSIMELLESVDMMAQNLPYRKFKPDVPLHHHAREWWAYAIHGVLEVNVCPRLWMWSWKHIRKHRQKVKQYKELYKKKLTSKKPPGELLVSLEELEKTLDVFNITIARQTAEVEVKKAGYKIYKEGVK-----------DPEDNKGWFSWLWSWSEQNTNEQQPDVQPETLEEMLTPEEKALLYEAIGYSETAVDPTLLKTFEALKFFVHLKSMSIVLRENHQKP-ELVDIVIEEFSTLIVQRPGAQAIKFETKIDSFHITGL--PDNSEKPRLLSSLDDA--MSLFQITFEINPLDETVSQRCIIEAEPLEIIYDARTVNSIVEFFRPPKEVHLAQLTAATLTKLEEFRSKTATGLLYIIETQKVLDLKINLKASYIIVPQDGIFSPTSNLLLLDLGHLKVTSKSRSELPDV--------KQGEANLKEIMDRAYDSFDIQLTSVQLLYSRVGDNWREARKLSVSTQHILVPMHFNLELSKAMVFMDVRMPKFKIYGKLPLISLRISDKKLQGIMELIESIPKPEPVTEVSAPVKSFQIQTSTSLGTSQISQKIIPLLELPSVSEDDSEEEFFDAPCSPLEEPLQFPTGVKSIRTRKLQKQDCSV--------------NMTTFKIRFEVPKVLIEFYHLVGDCELS------VVEILVLGLGAEIEIRTYDLKANAFLKEFCLKCPEYLD-ENKKPVYLVTTLDNTM-EDLLTLEYVKAEKNVPDLKSTYNNVLQLIKVNFSSLDIHLHTEALLNTINYLHNILPQ----SEEKSAPVST---------TETEDKGDVIKKLA---------------LKLSTNEDIITLQ------------------------------ILAELSCLQIFIQDQK-CNISEIKIEGLDSEMIMRPSETEINAKLRNIIVLDSDITAIYKKAVYITGKE-VFSFKMVSYMDATAGSAYT-DMNVVDIQVNLIVGCIEVVFVTKFLYSILAFIDNFQAAKQALAEATVQAAGMAATGVKELAQR--SSRMALDINIKAPVVVIPQSPVSENVFVADFGLITMTNTFHMITESQSSPPPVIDLITIKLSEMRLYRSRFINDAYQEVLDLLLPLNLEVVVERNLCWEWYQEVPCFNVNAQLKPMEFILSQEDITTIFKTLHGNIWYEKDGSASPAVTKDQYSATSGVTTNASHHSGGATVVTAAVVEVHSRALLVKTTLNISFKTDDLTMVLYSPGPKQASFTDVRDPSLKLAEFKLENIISTLKMYTDGSTFSSFSLKNC-ILDDKRPHVKKATPRMIGLTVGFDKKDMMDIKYRKVRDGCVTDAV-FQEMYICASVEFLQTVANVFLEAYTTGT-----------AVETSVQTWTAKEEVPTQES--VKWEINVIIKNPEIVFVADMTKNDAPALVITTQCEICYKGNLENSTMTAAIKDLQVRACPFLPVKRKGKITTVLQPCDLFYQTTQKGTDPQVIDMSVKSLTLKVSPVIINTMITITSAL--YTTKET-------IPEETASSTAHLWEKKDTKTLKMWFLEES-NETEKIAPTTELVPKG------------------------------EMIKMNIDSIFIVLEAGIGHRTVPMLLAKSRFSGEGKNWSSLINLHCQLELEVHYYNEMFGVWEPLLEPLEIDQTEDFRPWNLGIKMKKKAKMAIVESDPEEENYKVPEYKTVISFHSKDQLNITLSKCGLVMLNNLVKAFTEAATGSSADFVKDL-APFMILNSLGLTISVSPSDSFSVLNIPMA---KSYVLKNGESLSMDYIRTKDN----------DHFNAMTSLSSKLFFILLTPVNHSTADKIPLTKVGRRLYTVRHRESGVERSIVCQIDTVEGSKKVTIRSPVQIRNHFSVPLSVY------------EGDTLLGTASPENEFNIPLGSYRSFIFLKPEDENYQMCEGIDFEEIIKNDGALLKKKCRSKNPSKESFLINIVPEKDNLTSLSVYS------------EDGWDLPYIMHLWPPILLRNLLPYKIAYYIEGIENSVFT--LSEGHSAQICTAQLGKARLHLKL-LDYLNHDWKSEYHIKPNQQDISFVSFTCVTEMEKTDLDIAVHMTYNTGQTVVAFHSPYWMVN--------KTGRMLQYKADG--------------------------IHRKHPPNYKKPVLFSFQP-----------------NHFFNNN-------KVQLMVTDSELSNQFSIDTVGSHGAVKCKGLKMDYQVGVTIDLSSFNITRIVTFTPFYMIKNKSKYHISVAE-EGNDKWLSLDLEQCIPFWPEYASSKLLIQVERSED------PPKRIYFNKQENCILLRLDNELGGIIAEVNLAEHSTVITFLDYHDGAATFLLINHTKNEL-VQYNQSSLSEIEDSLPPGKAVFYTWADPVGSRRLKWRC---RKSHGEVTQKDDMMMPIDLGEKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAEQEIAVALQDVGISLVNNYTKQEVAYIGITSSDVVWETKPKKKARWKPMSVKHTEKLEREFKEY-----TESSPSEDKVIQLDTNVPVRLTPTGHNMKILQPHVIALRRNYLPALKVEYNTSAHQSSFRIQIYRIQIQNQIHGAVFPFVFYPVKPPKSVTMDSAPKPFTDVSIVMRSAGHSQISRIKYFKVLIQEMDLRLDLGFIYALTDLMTEAEVTENTEVELFHKDIEAFKEEYKTASLVDQSQV---------SLYEYFHISPIKLHLSVSLSSGREEAKDSKQNGGLIPVHS--LNLLLKSIGATLTDVQDVVFKLAFFELNYQFHTTSDLQSEVIRHYSKQAIKQMYVLILGLDVLGNPFGLIREFSEGVEAFFYEPYQGAIQGPEEFVEGMALGLKALVGGAVGGLAGAASKITGAMAKGVAAMTMDEDYQQKRREAMNKQPAGFREGITRGGKGLVSGFVSGITGIVTKPIKGAQKGGAAGFFKGVGKGLVGAVARPTGGIIDMASSTFQGIKRATETS-EVESLRPPRFFNEDGVIRPYRLRDGTGNQM-LQVMENGR-FAKYKYFTHVMI-NKTDMLMITRRGVLFVTKG-TFGQLTCEWQYSFDEFTKEPFIVHGRR-LRIEAKERVKSVF 3140          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. UniProt/SwissProt
Match: sp|Q5H8C4|VP13A_MOUSE (Vacuolar protein sorting-associated protein 13A OS=Mus musculus OX=10090 GN=Vps13a PE=1 SV=1)

HSP 1 Score: 720.309 bits (1858), Expect = 0.000e+0
Identity = 578/2046 (28.25%), Postives = 999/2046 (48.83%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETK-DSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFF-KSVKMDSLAVYWNSNPKPFS-SENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQV--LRAINVLPDSETASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIG--TTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTSV----VLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSN--FNEAINQVPSVPESKEEVKSRKILPKDDKSGSART---------ASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIR-NIKNDSSDFISIKFIQDKNSNSKLIVD--IAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTL--TAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPL---LEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIF---GAPYVVKNTCGKDV 6062
            MVFES+VVE++N++LGDYVV LD+SQL LG+  G   L+NL IKENA  + D+P KV+ GHI  L LKIPWK+LY++PV A L+ +++LI+P+S ++YDP KE   L ETKQ+EL RIE+ K K  D +K  E K D+F EKL  QI++NLQ+ IS+IHIRYED  T  ++  + GI+L N+S QT D      +   D+T +   K +++D+L  YWN N + F  ++   +LKA +KN I +         F+ +PIS SA + ++ + +  +F+ P+++L + L  I   FN+ QY  +   L+S+D       +RK++P+VP+  +   WW +  + ILE  V    RMWSW++I+ HR + + Y + +++KL  KK S     +++  E+ LD+ NIT+ RQQAE++AKK   K+ ++       +  +       S S A+   D     +    T EEK+ LY+AIGYSE   DP +P  + +LK  + L+ +  ++ +N  K  +L+ +V+  +S  V QR  A AI     +++  +TG    D   P L+S+   +   +LL + FE NPL+++  Q+  I AEPLEIIYD+ TVN IV+F  PP+++ L ++++  L  +EE +  TATGL ++ + +   D+++++K SY I+P+ G ++    +LL+DLG + + S+R S+       L   +  + + + +   AYD F I L++IQ L    GD+W++ R    S  HIL P      L K ++  D  +PK  + G LP + + ISD+ LQ ++EL+  IP      P+PV    +  R +   Q    P  +++   +  L       DSE     +            ++  ++   +    +KK  K   + N+I L   F +    +Q    +        G  E+  L +  +G  T    + FD+   A++  L L  P+++D   K PV L  T  +T  ++LL +  + VEK +P+  + + N    ++  F S+++ LH EALL  +N++N+++P L     EK+ + S+   E+                    K D  I+  +  ++    +  T++L AE            S  +IFI                       ++  NI +I I G+ +++      TE++  + ++ + D   +A+YKK ++I   E +FS ++  +   T+   Y  D+S VD  V++ VG I+++F+  F+  I  F+++FQ     +A    + +E AA+ +  +  +  SSR  LD+N+KAPV+ IP++  S N  + D G +T KN F     T      V++   I LS +R+ RS    +T +    +L P+N+ + + RNLSW     +P  +I A++  +   +SQ D       L  N  + + ++   S  +  E + S    P D    +  T         AS     +   F+ ++L + L+    +   +     P   L + K EN+     ++ + +   S  + N  + D +    K    +I   +  D  D + IK+ + K   + ++ D  +  +Y+C  V++LM +   F  A                      R  R+ P        L  +E+++ +  PEI+ V DM   D+ A+++ T   +       S+ +   I D Q   CPF    RK     ++ P  +     +   +     ++++ L + ++P +I +I  I + L  T ++  E +T +           + + W+K++    +  F+ +   E EK+   N+ +  G                                + LR+  I ++LE+ +  + VPML+ +    G+ KN L+ I L   + + V Y+N+   VWEPL   LE+   +  R ++  I+        + + +   +   +     +I   SRD L +T+SK  L +L+NL +AF +A +       S+F    AP+++ N+ G  V
Sbjct:    1 MVFESVVVEVLNRFLGDYVVNLDESQLSLGIWKGAVALKNLVIKENALHELDVPFKVKVGHIGSLKLKIPWKNLYTQPVEAVLEEIFLLIVPSSRIQYDPIKEEKQLMETKQQELKRIEKAKQKVFDKEKPREEKQDTFTEKLVTQIIQNLQVQISSIHIRYEDDITNGDKPLSFGISLQNISLQTTDQYWIPCL--HDNTEKLVRKLIRLDNLFAYWNVNSEMFYLNDYDESLKA-LKNGIVNENIVPEGYDFVFRPISASAKLQMNRRSD-FDFSDPKINLAVDLHTIAIEFNKPQYFSLMELLESIDMMTQNQPYRKFKPSVPLHLHAKEWWAYAIHSILEVNVCPSLRMWSWEHIRNHRYKMKRYREFYKKKLTSKKPSPEILMSLEELEKTLDVFNITIARQQAEVEAKKAGYKIYKEGVKDPEDNAGWFGWLWTWSESNANQQQDVKPGILEEMLTPEEKSLLYEAIGYSETAVDPTLPKTFEALKFFVHLKSMSIVLRENHQKP-ELLNVVVEGLSTSVVQRPGAQAIKFETKIDSFHITGLPD-DFKKPHLLSSLDDT---SLLQITFEINPLNETVAQRCTIEAEPLEIIYDARTVNSIVEFFRPPKDVHLAQLTSVTLTKLEEFRAKTATGLLYVIETQKVLDLRINVKASYVIVPQYGNFSPTSNLLLLDLGHLKVSSKRRSL-------LPDVRPSEASLEDIMHRAYDSFDIQLTSIQLLYSRVGDNWKEARKLNVSTQHILIPMHVNVELSKAMVFMDIKMPKFKISGKLPLVSLRISDKKLQGIMELLGSIP-----KPEPVTDVSAPARSFQ-IQASALPVSHISQKLIPLLEQPVTEDDSEEEFFDAPC-------SPLEECPQVSCRDKCTRQKKLQKKDCVMNLIQLRMRFEVAEVSIQFYHLV--------GDCELPVLEMGALGLGTEAEFRTFDLKGSAFLKELWLKCPEYLD-ENKKPVYLITTLDNTM-EDLLTLEFMKVEKNAPNLNSTYNNVLQLIKVNFSSLDIHLHTEALLNTMNYLNNILPEL----REKSASVSAAEPED--------------------KGD--IIKKLALKLPTNEDIITLQLLAEL-----------SCLQIFI----------------------QDQKQNISEIKIEGLDSEMIMKPLVTEINAKLRNIIVLDSDKMAIYKKALYITGKE-VFSFKMISYMDATAGYAYT-DMSVVDIRVHLTVGCIEVVFITKFLYSILAFIDNFQAVKDALAEATVQAAEMAADGVKELARK--SSRFALDVNIKAPVVLIPQSPVSQNVFVADFGLITMKNIFVTVTETQSNIPPVIDLITIKLSKMRLYRSQFRNDTYQEVLDLLLPLNLEVIVERNLSWEWYKEVPCFNIKAQLKPMEFILSQEDLTTVFQTLHGNIWYGQDLSAPSSANKDPETMTSGVTSPPDHSPATVVTAAVVEVHPQASQAHTMLNVSFQTDYLTMALYSPGPDEASFTDVRDPSLELAEFKLENIISSLKIYTDDSTVFSFSVKNCILDDKRSHVMKATPRMIGLTVGFDKKDMVDIKYRKIK---TFVVTDAVVQEMYVCASVEFLMTVAHIFFDAYMTSTALETSVQT---------RTTREAPAQ-----ELGKWEMNILIKNPEIVFVADMTRNDAPALVITTQCEICCKGEPTSNTVTAAIKDLQVRACPFLPVKRKGKVTTVLQPCDLFYQATQLGRDPQMIDISVKSLTLKVSPVIINTIITITSALYTTKETVPEENTSN-----------IAHLWDKKDTKNLKMWFLEES-NESEKVVPTNEVMPGG------------------------------ETLNLRIDSIFIVLEAGIGHRTVPMLLAKACFSGESKNWLSLINLHCHLELEVHYYNEMFGVWEPLLEPLEIDQTDDFRPWNLGIKMKKKAKEAIVESDSEAENYKVPEYKTAISFYSRDQLNITLSKCGLVMLNNLVEAFTEAAT----GSSSVFLRDLAPFMIFNSLGLTV 1881          

HSP 2 Score: 538.11 bits (1385), Expect = 4.780e-152
Identity = 404/1141 (35.41%), Postives = 599/1141 (52.50%), Query Frame = 3
Query: 6765 YKLDINVPLALHNQLPIKISY----------------QSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQSLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG--WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQ-ELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWN-QIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKK----LMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVS-GSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA--------LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITK 10088
            Y L +  P+ + N LP K++Y                 SQI++ ++    L L                 Y   +W     +    ++++ INF       +S +D+ +H+    GQ  V  +SPYWM+NKT   L YKA D    +H    T  VL                           +S Q                        N+  N N       K  L + DS  SD+FS+DTVGS G + CK   + Y+VGV INLSS  +T+IVTF+P+YMI N+S    Y   + E     W+ +   +++PFWP +    S ++  +VE  E    + +   + + IL++L  +  GI  EV + E + +I    Y +G A   LINH   D  + + Q +  + E  LP  + V Y W D    R+  W       + ++    D +    + +   Y VSF     RI+LFT D  V K    S   E  + E+ ++L  +G+SLV+N  +QE+AYI ITSS V W  + ++K R KP+ +  +E LE  F E+     T  + +E+ +V+++ + V L+       +++P    + R + P + ++Y TS HQ    ++IY+IQI NQ+    FP VF P  PPRSV +D+  KP  ++S V+  +G +   R KYF+VLIQEM L LD GFV ++ D V+   V    E   F KD +         S++       +  +F+ FHISPIK+HLS SLS      K +        +HS  LNL L+S+G  +TD+QDV+FKL +FE        +++ S +  HY  QA++QMYVL+LGLDVLGNPFG+IR  ++GVE  FYEP +GA+ GPEEFVEG+A G+K+L G AVGG AGA S+I   M KG+AA+T+D DYQ+KRRE + K+P  L  G+ +GGKGL+ GFV G+TG+VTKPI GA+K+G  GFFKGVGKGLVG VTRP  G++D ASS+F+GI+R  +T+ EV  +RPPRF  +DG+IR Y LR+  GN + +++ E         Y  HV   +K ++  +T + +  + +    G  + +WQ  +++    P I   +  +    KE+ + +F      K
Sbjct: 2096 YVLHLWPPILIRNLLPYKVAYYIEGIENTVVTLSEGHSSQIYNVEMDQAKLHLK-------------LLDYLNHDWKSEFYIRSSQQDINFINFTCLTEMEKSDLDIAIHMTYNTGQTVVAFHSPYWMVNKTNRMLQYKA-DGIHRKHPPNYTKPVL---------------------------FSFQP-----------------------NHFFNNN-------KVQLMVTDSELSDQFSIDTVGSHGAIRCKGLKMEYQVGVTINLSSFNITRIVTFIPFYMIKNKSK---YHISVAEEGSDKWLSLDLEQSIPFWPEN---ASNILLIQVERSEDPPKRIYFNKQDNCILLRLNNELGGIIAEVNLAEHSTVITFSDYHDGAATFLLINHTKSD-PVQYNQSSLGEIEDSLPPGKAVYYTWADPVGSRKLKWSCGQSYGEVTHK--DDMMTPISVGKKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAELEVVLALQDVGISLVNNYTKQEVAYIGITSSDVVWEAKPKKKARWKPMSVKHTEKLEKEFREY-----TEASPLEDKVVELDNIPVRLTPSGNDMKILQPHVIPVRRNYLPALKVEYNTSAHQSSFRIQIYRIQIQNQIHGAIFPFVFYPIKPPRSVTMDSAPKPFTDVSIVMRSAGHSQISRIKYFKVLIQEMDLSLDLGFVYALADLVTKAEVTEKTEVEHFHKDVEAFEQEYEVVSSVDQ----SQVNLFEYFHISPIKLHLSVSLSSGRDEAKDSEQHGGLIPVHS--LNLLLKSIGATLTDVQDVVFKLAFFELNYQFHTTSELQSEVIRHYSKQAIKQMYVLILGLDVLGNPFGLIREFSEGVEAFFYEPYQGAIQGPEEFVEGMALGLKALVGGAVGGLAGAASKITSAMAKGVAAMTMDEDYQQKRREAMNKQPAGLREGITRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVTRPTGGIIDMASSTFQGIKRATETS-EVESLRPPRFFNEDGVIRPYRLRDGSGNQM-LQVMENGR-FAKYKYFTHVMI-NKTDMFMITRRGVLFVTKG-TFGQLTCEWQYTFDEFTKEPFIVHGRR-LRIEAKERVKSVFHAKEFGK 3139          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. UniProt/SwissProt
Match: sp|Q8BX70|VP13C_MOUSE (Vacuolar protein sorting-associated protein 13C OS=Mus musculus OX=10090 GN=Vps13c PE=1 SV=2)

HSP 1 Score: 640.188 bits (1650), Expect = 0.000e+0
Identity = 483/1672 (28.89%), Postives = 819/1672 (48.98%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEE--------------------------------------------NKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQ-TAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSE--TASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQV----PSVPESKEEVKSRKI-LPKDDKSGSARTASD-------ESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIK-NDSSDFISIKFIQDK-------------NSNSKLIVDIAGIYMCICVDYLMMLTDFF 4808
            MV ES+V +L+N++LGDYV  L+KSQLKLG+ GGN  L+NL IKENA  + D+P KV+ G I KLTLKIPWK+LY E V+A L+GLY+L++P + +KYD EKE   L + KQKEL RIEE                                              L R+  K +   KD+F EKLA Q++KN+Q+ I++IHI+YED  T P R  + G+TL   S  T +   T  I+ E + +  +K VK+DSL+ YWN         +R  +   +K  I +S     D  ++ QPIS SA ++++   E+     P++  +++++ I     + QY  +  FL+S+D       +RKY+P +P+ +N   WW +  + +LE  +RR  + WSW  I+ HR   + Y   ++ KL   K+S+  Q  I+  E+ LD+ NI L RQQA+++     +KL +K++  G   GWF     +  S    D    + + +    T EEK KL+ AIGYSE   +  +P +YV+  L L+L     II +N     +++++ I  +   V QR  A A+ +   + +  VTG +  D  VP+LV++   +   +LL ++FETNP +  A+Q + ++++P+E+IYD+ T+N +V+F    + L L +I++  L  +EE+KE TATGL H+ + R   D+++++KPSY I+P+ G + +   +L++D G+  + S+      + +  L          + + + AYDKF + + ++Q L  +  ++W+K R Q  S  HIL+P      L K ++  D  + K  V G LP + V ISD+ ++  L LI  IP PQ ++ P P     SI        L    K  L ++ +L  +    D E   A    +      +  E K A E+ +                  ++ L   F I    L++ +  K         + I+   V ++GT  T++ FD+   +Y+  + L     +  S K P+ L  +     G +LL +  + V++  P F T    T   ++  F S+ + L  +ALL  LN++ ++IP                      + T + KE +A+ + ++      +M                       S  SD  G+    ++F            A L    V V +EK +NI +I I G+ + +        +   + ++ + D  P  ++KK + I  +E +F   + L+   T    Y  D+S VD +V + VG I+I++L+ F++ + +FLN+FQ + + ++    + +E AA  + ++ +   S R  +DI++KAPVI IP++S S NA++VDLG +   N F++    D +   V+++ ++ L+ L++SR+ +   T   +  +LHPIN+   ++RNL+ N  H +P + I  R+ S++V ++Q D N+   +L  N  EA   +    P + E  E    R++  P+D  +     A+        + I V   F+++ + V L K          E         +K  +L +E  +  +  M  +  L NI++       +K   E +R +  +D SD I +K +  K             N+  KL V  + + + + ++ L+ L DF 
Sbjct:    1 MVLESVVADLLNRFLGDYVENLNKSQLKLGIWGGNVALDNLQIKENALSELDVPFKVKAGQIDKLTLKIPWKNLYGEAVVATLEGLYLLVVPGASIKYDAEKEEKSLQDIKQKELCRIEEALQKAAEKGAHSGEFMYGLENLLYKDVKPGRKRKKHKKHFKKRFKGLDRSKDKPKEAKKDTFLEKLATQVIKNVQVKITDIHIKYEDDITDPERPLSFGVTLREFSLLTTNEHWTPCILNE-AEKIIYKLVKLDSLSAYWNVGCCMSYRGSREHILEQLKREILTSTNIPPDHQYIFQPISASAKLYMNPGAES-ELKTPKLDGNVEVQNIAIELTKPQYLSMIDFLESLDYMVRNAPYRKYKPCLPLHTNCRQWWKYAIDSVLEVHIRRYTQPWSWSNIKNHRQLLKSYKMAYKTKLTQAKVSEEIQKQIQDLEKSLDVFNIILVRQQAQVEVIHSGQKLRKKSAEAGEKRGWFSGFWGKKESK-KRDEESSVPETIDDLMTPEEKDKLFTAIGYSENAYNLALPKQYVAHILTLKLVSTSIIIRENR-NVPEILRVQIIGLGTQVSQRPGAQALKIEAKLEHWYVTGLRQQD-IVPSLVASIGDTT-SSLLKIEFETNPENSPADQTLIVQSQPVEVIYDAKTINAVVEFFQSNKGLDLEQITSATLMKLEEIKERTATGLTHIIETRKVLDLRINLKPSYLIIPQTGFHHEKSNLLILDFGTFQLNSKDQGAQKTANASL----------EEIIDKAYDKFDVEIRSVQLLFAKAEENWKKCRFQHPSTMHILQPMDIHVELAKAMVEKDVRMAKFKVSGGLPLMHVRISDQKIKDALCLINSIPLPQKSSTPSPERQVASI------PVLSGGTKALLGTSLLLDGVESESDEEFFDAEDGDSQAARTVKASELKKAAEVPNE----------------ELVSLLLKFEIKEVVLELTKQQKE-------EETILVFNVTQLGTEATMRTFDLTAVSYLRKISLDY-HDIKGSRKKPIHLISSSDRP-GLDLLKVEYIKVDRNGPSFQTTFEKTEQTVKVAFSSLNLLLQTQALLSSLNYLTTVIP-------------------SDSQNTGVAKEVQAMPEKQKNSPLQKVM---------------------VPSRDSDVIGF----RLF------------AKLNAFCVTVCDEK-SNIAEIKIQGLDSSLSLQSKKQSLFARLENIIVTDVDPKTIHKKAVSIVGNE-VFRFNLDLYPDATEGDSYT-DMSTVDGVVALHVGCIQIVYLHKFLMSLLSFLNNFQVAKEALSAATAQAAEKAATSVKDLAQR--SFRVSVDIDLKAPVIVIPQSSLSTNAVVVDLGLIRVHNRFSLVSGEDTANPPVIDKMEVQLTKLKLSRTAIQPGTSHPDIQLLHPINLEFFVSRNLAANWYHKVPVVEIKGRLDSMNVSLNQEDLNLLFRILAENLGEATEDLDKGKPRIQERGETKACREVSTPQDVHTTQGVPAARVEETRPVDIINVLLNFEIKEVVVTLMKKAERKGSPFHE---------LKILHLGMEAKVKAHD-MTAAAYLRNISMRCFHFPDSK--GEPLRIVNTSDVSDGILLKLLFIKADSDGPDFKTIHDNTKQKLKVSFSSLDLVLHLEALLSLMDFL 1552          

HSP 2 Score: 557.755 bits (1436), Expect = 9.066e-158
Identity = 390/950 (41.05%), Postives = 573/950 (60.32%), Query Frame = 3
Query: 7368 KNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG-----WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFY--ESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDF-YINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEG-LEVDLSKKLMIK--PEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEA------MFVKDCQMIRASLIETS-TIKTILSGGKKCVFDNFHISPIKIHLSFSL------SRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKS-KKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINES------KHTIVFTLKEKQ-----RKLFSKDNITKEIEADLI 10109
            K   F+K  K  LKI+ S WS+ FSLDTVGS G V C    + Y VGV I +SS  L+++VT  P+  + N+SS+ L   E+  +       W  V   E +PFWP +  GK  +     E      ++PF FY  + +  L+ LE    GI V++   E + +I    Y EG A   ++NH   DV + +KQ  + +EL+L   +T ++AW D    R+  W    +  +H  +L  D    F Y   ++ +WVSF+    R+LLFT+D+ +   A  + ++E  D E+ +SLHS+GLSLV+N  +QE++Y+ ITSSGV W +++ K + KP    Q  +LE A+ +   RL    A  +   VK++   EV+  K  M    P +C + R F  G+ ++++ SPHQ  +  R+Y +Q+DNQ+    FPVVF P  PP+S+ALD+  KP I++S +   +  +  +++KYF VLIQEM L +DQGF+ +++   +   PT D EA      +  +D   +   L+E+S T  +ILS      F++FHISP+K+HLS SL      S   +    A+HS  +NL L+S+G  +TD+ D+IFKL Y+E +      +Q++ S+  HY  Q L+QMYVLVLGLDVLGNPFG+IRG+++GVE LFYEP +GAV GPEEF EG+  GV+SL GH VGGAAG VSRI G++GKG+AA+T+D +YQ+KRRE++ ++P++ G  LA+GGKG + G V GVTG++TKP++GAKK+G  GFFKG+GKGLVG V RP  G++D ASS+F+GI+RVA++  EV  +RPPR I +DGIIR Y  +E+EG+++     +K +    E Y FH      K+ V+ +TN+R   +   EILG  S+ WQ  +E    PP ++E+      K   +F  K+       RK++ KD IT +   D I
Sbjct: 2802 KKNIFSKN-KVQLKISTSAWSNGFSLDTVGSYGCVKCPATNMEYLVGVSIKMSSFNLSRVVTLTPFCTVANKSSLDLEVGEIASDGSIPTNKWHYVASSECIPFWPENLSGKLCVRVVGYEGS----SKPF-FYNRQDNGTLLSLEDLNGGILVDINTAEHSTVITFSDYHEGSAPALIMNHTQWDV-LTYKQSGSQEELVLLPGETRLFAWADPTGIRKLTWNYAANFGEH--DLLKDECGQFPYDANIQIHWVSFLDGRQRVLLFTDDVALVSKALQAEEMEQADHEVALSLHSLGLSLVNNENKQEVSYVGITSSGVVW-EMKPKQKWKPFSQKQIMSLEQAYSK---RL----ASQDRGWVKLDSNFEVNFDKVPMEMRLPIRCPIKRDFLSGIQVEFKQSPHQRSLRARLYWLQVDNQLPGTMFPVVFHPVAPPKSIALDSEPKPFIDVSVITRFNEYSKVLQFKYFMVLIQEMALKVDQGFLGAVISLFT---PTTDPEAERKRTKLIQQDIDALNTELMESSMTDMSILS-----FFEHFHISPVKLHLSLSLGSGGEESDKEKQEMIAIHS--VNLLLKSIGATLTDVDDLIFKLAYYEIRYQFYKRDQLMWSVVRHYSEQFLKQMYVLVLGLDVLGNPFGLIRGLSEGVEALFYEPFQGAVQGPEEFAEGLVIGVRSLVGHTVGGAAGVVSRITGSVGKGLAAITMDKEYQQKRREEMGRQPKDFGDSLARGGKGFLRGVVGGVTGIITKPVEGAKKEGAAGFFKGIGKGLVGAVARPTGGIIDMASSTFQGIQRVAESTEEVSSLRPPRLIHEDGIIRPYDRQESEGSDLLENHIKKLE---GEAYQFHCAVPGNKRAVLMITNRRALFIKEVEILGHMSVDWQCLFEDFVCPPEVSENLLKISVKEQGLFHKKDSANIGHLRKIYLKDPITAKRAFDAI 3721          

HSP 3 Score: 341.273 bits (874), Expect = 5.155e-92
Identity = 352/1494 (23.56%), Postives = 663/1494 (44.38%), Query Frame = 3
Query: 2826 IVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTS--PDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTSVVL----EESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSG----------SARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELI--RNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXM-VPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFE--IEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDA------YSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADS--ISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKC---YNVPIPTLLTSNGKIYIS-VKQLNTEISNSGAL--WNIGKNAQPSDTQILLCKS---NTGVMYYYSVALSIEEVQIVNSSETLR-TYKLDINVPLALHNQLPIKISY--QSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAV 7184
            I+H +G    +K  DM   AY+ ++ +    F D S   P+ +  T   + G   + + LL ++  S  PDF T+H NT+ KL+  F S+++ LH EALL +++F++S                       +   ++ EKE E    LK                 L  E R++ ++A   S + D   +D                ITA L    + + ++K +NI +I IHGM A +      T+V   + ++ + +   L+++KK + I  DE +F  Q++L+   T    Y  D+S+VD  ++++VG I+I++++ F + + +FLN+FQ + + ++    + +E AA+ + ++ ++  S R  +DI++KAPVI IP++S S N ++ DLG +  +N F++     + L    +E  I L+ L+++R++L  ++ +++  IL P+N+ + + RNLS      IP + I   +  + V +SQ D  +   +L  N  EA +Q PS  +  +E    K   +D +SG            +   D+++T+QF F  + L + L+  D++     S       L ++    ++  G M  + +M VS++L    + D +    +  + +I  +N ++++S  I I + QDKN  S++   +  +Y+C  V++LM + DFF+KA P   +   K + +P R       K  K     P      +  +   +  PE++ V  +   D+ A+       L  S  K   ++   + D + + CPF  E R K+   ++ P S+ +      +     ++ ++  +I I+P ++ ++  IM  ++ +      T++  + +   +   DN W  +   +    F+               GV+   +V +   D E                  E    + +  ++V LE  +  + VP+L+ E    G++KN  + +     +++ V Y+N+   VWEPL+E    E N+ +S +  ++     D  L   +DF     I     ++ + S   + +T+SKS L+V SNL K F +       YSL          AP+ VKN  G  V   +    +LK   S E+  +Y    G+    D    + S     +Q K+      +S   +  ++    TEV ++  +       +  N S    ++++V   A  G K + LRS LQ+ N+      +Y            KN  LLE +   +    ++VP+ +      ++Y+     L  + ++S     W    +       +L C +   +   +   +VAL  E   I    E     Y + +  PL L N LP  + Y  +    +H++  G    V  + +  +    V   Y  K W G   +   + E  ++ F +      + +DL VH+     +  ++++SPYW++NKT   L Y++ ++  V+H A   D +
Sbjct: 1452 ILH-LGMEAKVKAHDMTAAAYLRNISMRCFHFPD-SKGEPLRIVNTSDVSDG---ILLKLLFIKADSDGPDFKTIHDNTKQKLKVSFSSLDLVLHLEALLSLMDFLSS--------------------AIPSSDSSSSEKEPE----LK----------------PLVGESRSLAIRAVPSSYEGD--AFD--------------LKITAELNAFNIFICDQK-SNIAEIKIHGMDASISVKPKQTDVFARLKNIIVMNVDSLSIHKKAVSILGDE-VFRFQMSLYPDATEGENYG-DMSKVDGRLSLKVGCIQIVYVHKFFMSLLSFLNNFQAAKEALSTATVQAAERAASSVKDLAQK--SFRLLMDIDLKAPVITIPQSSVSPNVVIADLGLIRVENKFSLVSVEQLALPPVADEMSIQLTQLKLARTVLQADSPQHDVEILKPVNMLLCIQRNLSAAWYTQIPGMEIKGELKPMQVALSQDDLTVLMKILLENLGEASSQ-PSPTQYAQEAARVK---RDTRSGPDYLKEQELADPKPPGDQTVTLQFDFHFDSLSIILYNSDSSQEPRLSFHNDSFRLGELTLHLMASAGKMFKDGSMNVSLKLKTCTLDDLREGIERATSRMIDKKNDQDNNSSMIDISYSQDKN-GSQVDAVLDKLYVCASVEFLMTVADFFIKAMPQSPENIAKEIQIPSRQTAAGRVKMEKDDSVRP------NMTLKAMITDPEVVFVASLTKADAPALTASFQCNLSLSTSKLEQMMEASVRDLKVLACPFLRERRGKSITTVLQPCSLFMEKCTWASGKQNINIVVKEFVIKISPIILNTVMTIMAAMSPK------TKEDEWKDTPKE--TDNLWAVKSITDYNSWFL---------------GVDMATEVTENFRDSEHPSI--------------EENCVVAVESVQVTLECGLGHRTVPLLLAESKFSGNIKNWTSLMAAAADMTLEVHYYNETHAVWEPLIERV--EGNKPWSLKLNVKKNPIQDKSLMPGDDF-----IPEPQTAVHISSGATMNITISKSCLNVFSNLAKGFSEGAASTFDYSLKDR-------APFTVKNALG--VPMKVQPNRNLKVMGSPEKSDIYDVGAGQHLELDYASLEPS-----RQGKLSILSRQESSLFTLTFVPYGYTEVASVPVARPGRRLYNVRNPSASHSDSVLVQIDATEGNKVVTLRSPLQIKNHFSIAFIIYKF---------VKNVKLLERIGIARPEEEFHVPLDSY---RCQLYVQPAGGLEQQYTHSSTYISWKEELHRSREVRCMLQCPAVEVSFLPLIVNTVALPDELSYIGAHGEDWDPAYVIHLYPPLTLRNLLPYSLRYLLEGTAETHELAEGSSADVLHSRISGEIIELVLVKYLGKNWNGHFRICDTLPEFFLVCFSSDTAEVMT-VDLSVHVRRIGCRMELSVFSPYWLINKTSRVLQYRS-EEIHVKHPADFRDII 2796          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. UniProt/SwissProt
Match: sp|Q709C8|VP13C_HUMAN (Vacuolar protein sorting-associated protein 13C OS=Homo sapiens OX=9606 GN=VPS13C PE=1 SV=1)

HSP 1 Score: 630.172 bits (1624), Expect = 5.817e-180
Identity = 442/1499 (29.49%), Postives = 752/1499 (50.17%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEE--------------------------------------------NKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSE--TASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIP----VLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQV----PSVPESKE 4346
            MV ES+V +L+N++LGDYV  L+KSQLKLG+ GGN  L+NL IKENA  + D+P KV+ G I KLTLKIPWK+LY E V+A L+GLY+L++P + +KYD  KE   L + KQKEL+RIEE                                              L R+  K +   KD+F EKLA Q++KN+Q+ I++IHI+YED  T P R  + G+TL  LS  T +   T  I+ E + +  +K +++DSL+ YWN N       +R  +   +KN I +S     +  ++ QPIS SA ++++   E+     P++  +++++ I     + QY  +   L+S+D       +RKY+P +P+ +N   WW +  + +LE  +RR  +MWSW  I+ HR   + Y   ++ KL   K+S+  Q  I+  E+ LD+ NI L RQQA+++  +  +KL +K++  G   G +         S   D    + + +    T EEK KL+ AIGYSE T +  +P +YV+  + L+L      I +N     +++K+ I  +   V QR  A A+ V   + +  +TG +  D  VP+LV++   +   +LL + FETNP D  A+Q + ++++P+E+IYD+ TVN +V+F    + L L +I++  L  +EE+KE TATGL H+ + R   D+++++KPSY ++P+ G + +   +L++D G+  + S+   +  + ++ L          + + + AYDKF + +  +Q L     + W+K R Q  S  HIL+P      L K ++  D  + +  V G LP + V ISD+ ++ VL L+  IP PQ +  +      S +   SG   G+     L ++ +L  +    D E   A           +  E K A E+ +                  +I+L   F I    L+  +  K           I+   V ++GT  T++ FD+ V +Y+  + L   + ++ S + P+ L  +     G +LL +  +  +K  P F T    T   ++  F S+ + L  +AL+  +N++ ++IP     +++ +E +  T      E+ +K +TL K   +  D              +D ID                            ++F            A L    VIV NEK NNI +I I G+ + +        +   + ++ + D  P  V+KK + I  +E +F   + L+   T    Y  D+S+VD ++++ VG I+I++L+ F++ +  FLN+FQ + ++++    + +E AA  + ++ +   S R  ++I++KAPVI IP++S S NA++VDLG +   N F++    D     V++   + L+ L + R+++       +  +LHPIN+   + RNL+ +  H +P + I   + S++V ++Q D N+   +L  N  E    +    P V E+ E
Sbjct:    1 MVLESVVADLLNRFLGDYVENLNKSQLKLGIWGGNVALDNLQIKENALSELDVPFKVKAGQIDKLTLKIPWKNLYGEAVVATLEGLYLLVVPGASIKYDAVKEEKSLQDVKQKELSRIEEALQKAAEKGTHSGEFIYGLENFVYKDIKPGRKRKKHKKHFKKPFKGLDRSKDKPKEAKKDTFVEKLATQVIKNVQVKITDIHIKYEDDVTDPKRPLSFGVTLGELSLLTANEHWTPCILNE-ADKIIYKLIRLDSLSAYWNVNCSMSYQRSREQILDQLKNEILTSGNIPPNYQYIFQPISASAKLYMNPYAES-ELKTPKLDCNIEIQNIAIELTKPQYLSMIDLLESVDYMVRNAPYRKYKPYLPLHTNGRRWWKYAIDSVLEVHIRRYTQMWSWSNIKKHRQLLKSYKIAYKNKLTQSKVSEEIQKEIQDLEKTLDVFNIILARQQAQVEVIRSGQKLRKKSADTGEKRGGWFSGLWGKKESKKKDEESLIPETIDDLMTPEEKDKLFTAIGYSESTHNLTLPKQYVAHIMTLKLVSTSVTIRENK-NIPEILKIQIIGLGTQVSQRPGAQALKVEAKLEHWYITGLRQQD-IVPSLVASIGDTT-SSLLKIKFETNPEDSPADQTLIVQSQPVEVIYDAKTVNAVVEFFQSNKGLDLEQITSATLMKLEEIKERTATGLTHIIETRKVLDLRINLKPSYLVVPQTGFHHEKSDLLILDFGTFQLNSKDQGLQKTTNSSL----------EEIMDKAYDKFDVEIKNVQLLFARAEETWKKCRFQHPSTMHILQPMDIHVELAKAMVEKDIRMARFKVSGGLPLMHVRISDQKMKDVLYLMNSIPLPQKSSAQSPERQVSSIPIISGGTKGL-----LGTSLLLDTVESESDDEYFDAEDGEPQTCKSMKGSELKKAAEVPNE----------------ELINLLLKFEIKEVILEFTKQQKE-------EDTILVFNVTQLGTEATMRTFDLTVVSYLKKISLDYHE-IEGSKRKPLHLISSSDKP-GLDLLKVEYIKADKNGPSFQTAFGKTEQTVKVAFSSLNLLLQTQALVASINYLTTIIPSDDQSISVAKEVQIST------EKQQKNSTLPKAIVSSRD--------------SDIIDF---------------------------RLF------------AKLNAFCVIVCNEK-NNIAEIKIQGLDSSLSLQSRKQSLFARLENIIVTDVDPKTVHKKAVSIMGNE-VFRFNLDLYPDATEGDLYT-DMSKVDGVLSLNVGCIQIVYLHKFLMSLLNFLNNFQTAKESLSAATAQAAERAATSVKDLAQR--SFRVSINIDLKAPVIVIPQSSISTNAVVVDLGLIRVHNQFSLVSDEDYLNPPVIDRMDVQLTKLTLYRTVIQPGIYHPDIQLLHPINLEFLVNRNLAASWYHKVPVVEIKGHLDSMNVSLNQEDLNLLFRILTENLCEGTEDLDKVKPRVQETGE 1390          

HSP 2 Score: 550.821 bits (1418), Expect = 1.060e-155
Identity = 383/920 (41.63%), Postives = 561/920 (60.98%), Query Frame = 3
Query: 7395 KAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG-----WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFY--ESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDF-YINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEG-LEVDLSKKLMIK--PEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEA------MFVKDCQMIRASLIETS-TIKTILSGGKKCVFDNFHISPIKIHLSFSL------SRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCK-SKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDN 10079
            K  LKI+ S WS  FSLDTVGS G V C    + Y VGV I +SS  L++IVT  P+  I N+SS+ L   E+  +       W  +   E +PFWP S  GK  +     E      ++PF FY  + +  L+ LE    GI V+V   E + +I    Y EG A   ++NH   D+ + +KQ  +P+E++L   Q  ++AW D    R+  W    +  +H  +L  DG   F Y   ++ +WVSF+    R+LLFT+D+ +   A  + ++E  D EIT+SLHS+GLSLV+N  +QE++YI ITSSGV W +++ K + KP    Q   LE ++       +  +   +   +K++   EV+  K  M    P +  + R F  G+ ++++ S HQ  +  R+Y +Q+DNQ+    FPVVF P  PP+S+ALD+  KP I++S +   +  +  +++KYF VLIQEM L +DQGF+ +I+   +   PT D EA      +  +D   + A L+ETS T  +ILS      F++FHISP+K+HLS SL      S   +    A+HS  +NL L+S+G  +TD+ D+IFKL Y+E +      +Q++ S+  HY  Q L+QMYVLVLGLDVLGNPFG+IRG+++GVE LFYEP +GAV GPEEF EG+  GV+SLFGH VGGAAG VSRI G++GKG+AA+T+D +YQ+KRRE+L+++P + G  LA+GGKG + G V GVTG++TKP++GAKK+G  GFFKG+GKGLVG V RP  G+VD ASS+F+GI+R A++  EV  +RPPR I +DGIIR Y  +E+EG+++   +      +  E Y +H     SKK ++ +TN+R+  +   EILG+  + WQ  +E    PP+++E+    V  +  K++ LF K +
Sbjct: 2815 KVQLKISTSAWSSSFSLDTVGSYGCVKCPANNMEYLVGVSIKMSSFNLSRIVTLTPFCTIANKSSLELEVGEIASDGSMPTNKWNYIASSECLPFWPESLSGKLCVRVVGCEGS----SKPF-FYNRQDNGTLLSLEDLNGGILVDVNTAEHSTVITFSDYHEGSAPALIMNHTPWDI-LTYKQSGSPEEMVLLPRQARLFAWADPTGTRKLTWTYAANVGEH--DLLKDGCGQFPYDANIQIHWVSFLDGRQRVLLFTDDVALVSKALQAEEMEQADYEITLSLHSLGLSLVNNESKQEVSYIGITSSGVVW-EVKPKQKWKPFSQKQIILLEQSY-------QKHQISRDHGWIKLDNNFEVNFDKDPMEMRLPIRSPIKRDFLSGIQIEFKQSSHQRSLRARLYWLQVDNQLPGAMFPVVFHPVAPPKSIALDSEPKPFIDVSVITRFNEYSKVLQFKYFMVLIQEMALKIDQGFLGAIIALFT---PTTDPEAERRRTKLIQQDIDALNAELMETSMTDMSILS-----FFEHFHISPVKLHLSLSLGSGGEESDKEKQEMFAVHS--VNLLLKSIGATLTDVDDLIFKLAYYEIRYQFYKRDQLIWSVVRHYSEQFLKQMYVLVLGLDVLGNPFGLIRGLSEGVEALFYEPFQGAVQGPEEFAEGLVIGVRSLFGHTVGGAAGVVSRITGSVGKGLAAITMDKEYQQKRREELSRQPRDFGDSLARGGKGFLRGVVGGVTGIITKPVEGAKKEGAAGFFKGIGKGLVGAVARPTGGIVDMASSTFQGIQRAAESTEEVSSLRPPRLIHEDGIIRPYDRQESEGSDL---LENHIKKLEGETYRYHCAIPGSKKTILMVTNRRVLCIKEVEILGLMCVDWQCPFEDFVFPPSVSEN----VLKISVKEQGLFHKKD 3701          

HSP 3 Score: 317.39 bits (812), Expect = 7.976e-85
Identity = 317/1296 (24.46%), Postives = 578/1296 (44.60%), Query Frame = 3
Query: 3441 ITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKI--------LPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELI--RNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXX--XXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDA------YSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKP-CNSDEEFKVYLKADG---EVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDN---EGPSRGNVSEREIN-----TLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKC---YNVPIPT-----LLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKS---NTGVMYYYSVALSIEEVQIVNSSETLR-TYKLDINVPLALHNQLPIKISY--QSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAV 7184
            ITA L    V V ++K  NI DI IHGM A +      T+V   + D+ + +    +++KK + I  DE +F  Q+ L+   T    Y  D+S+VD  ++ +VG I+I++++ F + +  FLN+FQ + + ++    + +E AA+ + ++ ++  S R  +DIN+KAPVI IP++S S NA++ DLG +  +N F++      S   V+++  I L+ L++SR+IL  +  +N+  IL P+N+ + + RNL+      IP + I  ++  + V +S+ D  +   +L  N  EA +Q       +E V+ RK+        L + +    ++ + ++ +++QF F  E L + L+  D N     +       L +++   ++  G M  + +M VSV+L    + D +    +  + +I  +N ++++S  I I + QDKN  S++   +  +Y+C  V++LM + DFF+KA P        +  ++P R       K  K     P      +  +   +  PE++ V  +   D+ A+       L  S  K   ++   + D + + CPF  E R KN   ++ P S+ +      +     ++ ++  II I+P ++ ++  IM  L+ ++ +     DGS   +++   ++N W               G+      NT   GV++  ++ +    +E                  E    + +  I+V LE  +  + VP+L+ E    G++KN  + +     V++ V Y+N+   VWEPL+E    +       +++     D  L   +DF     I    ++I + S + + +T+SKS L+V +NL K F +       YSL          AP+ VKN  G  +         +KP CN        L+  G   +  ++D VD  Q+L  E         G    +SR+  S +   +    ++   N     P R   + R  N     +++V   A  G K + LRS LQ+ N+      +Y            KN  LLE +   +    ++VP+ +      +   G +    K+  T IS     W    +       +L C S   +   +   +VAL  E   I    E     Y + +   L L N LP  + Y  +    +H++  G    V  + +  +    V   YQ K W G   +   + E   + F + +    + +DL VH+     +  ++++SPYW++NKT   L Y++ +D  V+H A   D +
Sbjct: 1601 ITAELNAFNVFVCDQK-CNIADIKIHGMDASISVKPKQTDVFARLKDIIVMNVDLQSIHKKAVSILGDE-VFRFQLTLYPDATEGEAYA-DMSKVDGKLSFKVGCIQIVYVHKFFMSLLNFLNNFQTAKEALSTATVQAAERAASSMKDLAQK--SFRLLMDINLKAPVIIIPQSSVSPNAVIADLGLIRVENKFSLVPMEHYSLPPVIDKMNIELTQLKLSRTILQASLPQNDIEILKPVNMLLSIQRNLAAAWYVQIPGMEIKGKLKPMQVALSEDDLTVLMKILLENLGEASSQPSPTQSVQETVRVRKVDVSSVPDHLKEQEDWTDSKLSMNQIVSLQFDFHFESLSIILYNNDINQESGVAFHNDSFQLGELRLHLMASSGKMFKDGSMNVSVKLKTCTLDDLREGIERATSRMIDRKNDQDNNSSMIDISYKQDKN-GSQIDAVLDKLYVCASVEFLMTVADFFIKAVPQSPENVAKETQILP-RQTATGKVKIEKDDSVRP------NMTLKAMITDPEVVFVASLTKADAPALTASFQCNLSLSTSKLEQMMEASVRDLKVLACPFLREKRGKNITTVLQPCSLFMEKCTWASGKQNINIMVKEFIIKISPIILNTVLTIMAALSPKTKE-----DGSKDTSKE---MENLW---------------GIKSINDYNTWFLGVDTATEITESFKGIEHSLI--------------EENCGVVVESIQVTLECGLGHRTVPLLLAESKFSGNIKNWTSLMAAVADVTLQVHYYNEIHAVWEPLIERVEGKRQWNLRLDVKKNPVQDKSLLPGDDF-----IPEPQMAIHISSGNTMNITISKSCLNVFNNLAKGFSEGTASTFDYSLKDR-------APFTVKNAVGVPI--------KVKPNCN--------LRVMGFPEKSDIFD-VDAGQNLELEYASMVPSSQGNLSILSRQESSFFTLTIVPHGYTEVANIPVARPGRRLYNVRNPNASHSDSVLVQIDATEGNKVITLRSPLQIKNHFSIAFIIYKF---------VKNVKLLERIGIARPEEEFHVPLDSYRCQLFIQPAGILEHQYKESTTYIS-----WKEELHRSREVRCMLQCPSVEVSFLPLIVNTVALPDELSYICTHGEDWDVAYIIHLYPSLTLRNLLPYSLRYLLEGTAETHELAEGSTADVLHSRISGEIMELVLVKYQGKNWNGHFRIRDTLPEFFPVCF-SSDSTEVTTVDLSVHVRRIGSRMVLSVFSPYWLINKTTRVLQYRS-EDIHVKHPADFRDII 2801          

HSP 4 Score: 56.9954 bits (136), Expect = 4.458e-6
Identity = 36/120 (30.00%), Postives = 66/120 (55.00%), Query Frame = 3
Query: 2793 EKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIP 3152
            EK G + + +L V ++G    +K +DM  +AY+  + +    F D S   P+ +  +   T  + LL + L   +   P+F T+H +T+ +L+  F S+++ LH EALL  ++F++S  P
Sbjct: 1443 EKKG-RPLHELNVLQLGMEAKVKTYDMTAKAYLKKISMQCFDFTD-SKGEPLHIINSSNVTD-EPLLKMLLTKADSDGPEFKTIHDSTKQRLKVSFASLDLVLHLEALLSFMDFLSSAAP 1559          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. UniProt/SwissProt
Match: sp|A1Z713|VPS13_DROME (Vacuolar protein sorting-associated protein 13 OS=Drosophila melanogaster OX=7227 GN=Vps13 PE=1 SV=2)

HSP 1 Score: 523.857 bits (1348), Expect = 1.286e-147
Identity = 353/907 (38.92%), Postives = 534/907 (58.88%), Query Frame = 3
Query: 7368 KNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG-WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLE-KKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEM-KAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIR-RKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIR---ASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLF 10067
            +++ F    KA ++I++  WS+K  LD  GS G+V+C      Y VGV  +L+ + LTK +TF+P+Y++ N+    +   E    A  W+ + P E  P WPR++   ++++R   +     +T  F F E    L+KLE  K+ GI V+VQ TE    I    Y+   A   LINH G  +  H K   T  E IL A  T+MYAW D    +  ++   G  K+ ++ L  DGI +  + +  K  WVSF+    R+LLFT +  +A     +A L+SI Q I + +H IGLS+++N    ++ Y+ +TSSG+ W   +  KNR K L + ++  LE  + +++  +  S  +V+ + +      +D    ++ K  +  L R F P ++L  ++SP Q  +H++I +IQ+DNQ  D  FPVV AP PPP+SVA  T LKP IE S V      +   ++KY R+LIQE    +D  F+ +I +  +  V        F +D + I    ++  E  +++      +K  +DN H+ P+KIH+SFS++       T      L   +Q VG+ +TD+ DV+F+L +FE++       Q+++ ITSHY  QAL+Q+YVLVLGLDVLGNP+G++ G+ +GVEDLFYEP +GA+ GP EF EG+  GVKSLFGH VGGAAGAVS+I G MGKG+AALT D DYQ+KRR+ +  +P+N   GLA+  KGL++GFV GVTGVVTKP+ GA+ +G+EGFFKG+GKG +G+V RP +GVVDFAS SFE ++R AD + +V R+RPPRF   D ++R Y L EA GN I +K T+K      + ++       K   + +TN R+ ++ RNE+ G+W+  W   + ++    ++  +   + FT+K   +K+ 
Sbjct: 2394 RDKLFFDKKKASIRIDNGQWSEKIPLDVAGSVGEVICFANNQKYPVGVHNHLTQNSLTKQITFIPFYIVCNKCHFDIELQEQSRPADPWLHLEPNEMEPLWPRNDTKNNLVVRVDGK-----ITPAFDFTEVICTLLKLEDSKYGGINVDVQTTEGGVYITFTDYKPADAPGLLINHTGKQIVYHEK--GTKNEHILNAKSTIMYAWDDPTGPKMLVF---GTNKEETD-LKRDGIGEVIMQDGGKVLWVSFLDGLQRVLLFTENESIANRTESTASLQSITQSIDLRIHGIGLSVINNETGLDILYLGVTSSGIIWESKKVTKNRFKELTINENALLEIEYQKYL--VHKSVNDVQTYKLD-NKFPIDFDLMILKKTVERNLRRSFYPAIWLSRKSSPFQSQLHVKINRIQVDNQFLDPIFPVVLAPIPPPKSVASTTSLKPFIECSMVQRIMPNSTVRQFKYARILIQEFLFKVDLNFLTAIAEMFAKEVSDEAAAKQFRQDVESIELPLSAFFEEHSLEE-----QKSFYDNLHLGPLKIHVSFSMA----GSDTKALPGFLGSLVQGVGVTLTDVNDVVFRLAFFEREYQFFSQKQLINEITSHYTGQALKQLYVLVLGLDVLGNPYGLVVGLKKGVEDLFYEPFQGAIQGPGEFAEGLVLGVKSLFGHTVGGAAGAVSKITGAMGKGLAALTFDEDYQKKRRQGIQNKPKNFHEGLARSSKGLVMGFVDGVTGVVTKPVTGARDNGVEGFFKGLGKGAIGLVARPTAGVVDFASGSFEAVKRAADASEDVKRMRPPRFQHYDFVLRPYCLMEATGNKI-MKETDKGKFATTDNFIHCEEIIQKSEYLVVTNYRVMYVQRNEMFGVWTSLWSYLWNEIS---SVAATARGVQFTVKTDGKKVL 3273          

HSP 2 Score: 471.085 bits (1211), Expect = 1.459e-131
Identity = 276/829 (33.29%), Postives = 468/829 (56.45%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIE---ENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTN--FTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRP-NVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFII------SDNSLKEY-KLMKLVIHSMS---AHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKY-TKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRK-LRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPP 2456
            MVFE++V +++NK LGDY+  LD++QLK+G+ GG+  L+NL I+ENA D+ DLPV++  G++ KL LKIPWK+LYS+PVI  ++ LYVL+ PN+ ++Y+ EKE  Y  + K+  L+ +E   + +L+ + PK +      F EKL AQIV NLQ+ I+N+H+RYEDT+T     F+ GI+L  L   T D       + + ++ + FK   +  L+ Y N   + +++ N++ L    K NI+  E K  + ++++ PISC+A + ++M PE  +  F  P++ L L++E++       Q+ ++    D+M+R      +RKYRP N+P + +   WWHF    ILEE+VR+ R  W+W +I+ HR RC  Y   ++++ L KK S    +T ++ E ELD+ N+ L RQ+  ++  K R+ + ++ S      GW G +  +  ++     S +L +K  +  T EEK K+Y+AIGY E     D+P  Y ++++N +L  L+  +      S  + K++ +L  LV+ + S   A + QR +A+AIS+   M  ++VTG    D + P LV ++   +  NLL V FETNPLDK  +Q++ + A PL+I YD+ T+  ++     P ++ L +        I   KE +ATG++++ D++   D+ + + P+  ++P +G Y   +  +L+V +G + + S+              G+D       + E AYD+F + +  +Q L++  G+ W+  L    S++ H+LRP   +     CV+ ND  LP I V   LP + V +S+  +   +++   IP P+   P
Sbjct:    1 MVFEAVVADVLNKVLGDYIENLDRNQLKIGIWGGDVVLQNLKIRENALDELDLPVQLIYGYLGKLVLKIPWKNLYSQPVIVNIEDLYVLVSPNNNVQYNAEKEAKYEMDLKKAALDALEAARKKELEMDQPKADA----GFAEKLTAQIVNNLQVQITNVHLRYEDTTTT-GSPFSFGISLHELELYTTDCDWEKCYMAQQAS-QVFKIANLSCLSAYLNCGGQLYAN-NKSDLSQQFKTNIACKETK-PNYNYVLGPISCNAKLKLNMNPELDDPPFEKPKIDLTLEMEKLNVGLTNTQFDNLMKLGDAMNRQQLGIPYRKYRPYNIPYKGHARDWWHFAITSILEEEVRKPRESWTWGHIKTHRERCNTYAQKYKEQCLSKKPSAVLTETCRLLETELDVFNLLLIRQRVNIEIAKQREAVPEQKSGWFSGWGWGGGAKKDDQTT-----SQKLVEKFEAAMTSEEKEKMYRAIGYQENAKPTDLPESYEAIRMNFKLIALEVGLYKDERNSSAATKDFHELPSLVLLNFSMATALITQRPAAEAISIIAGMREIKVTGLTRNDYT-PLLVESKITDEF-NLLEVFFETNPLDKLCDQRVKVVARPLQITYDAPTILALINAFQTPGDVTLSKFEDAASTKISNFKERSATGMQYMIDKKAVLDVDILLMPNILVVPHKGVYDAGNVSLLVVSMGQVHLSSQPRRESNKLQHLFSAGEDKDEILKTVMENAYDRFTVAVDDVQMLVVRAGEPWQNALAEANSTEMHVLRPVSLKVTAALCVVDNDPRLPNIKVDIDLPAILVNVSEDRIFLAIKVATSIPLPEQKEP 814          

HSP 3 Score: 223.402 bits (568), Expect = 2.509e-56
Identity = 264/1102 (23.96%), Postives = 502/1102 (45.55%), Query Frame = 3
Query: 2961 TKKHTKGQNLLF-INLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMT-QWAITASLQKLRVIVANEKDNNILDISIHGMRAD--VKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKND----FAIDQSTSVVLEESKITLSNLRVSRSILLENT------VKNEKGIL------HPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESI---------TVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKI----------------NELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXM-VPVRGYEKNPRKYRKKP--LAS----PVEPRLAD-----FEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLL---EVYPEEHNRQYS-FEIEYISCDDSGLFD-EEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINK 6080
            T K     N LF ++  I +K+SP+F T + +T   +   F+ +++ LHQE L  ++  +N+    L++V               TR +  +                 SI G   D I     KRT+ +  E   E    +     KK     +  T +  + A+L ++ +++   K   I ++++    +   +K+SY  TEV+I + D+++ D +P  ++K I+ I   ++ F+ Q+ ++N+   T  Y  D    D  + +++G +KIIFL  FV  +  FLN+F  +  T++      +E+A  K  +  E   ++R KL+I +KAP+I +P  S+  NAL++DLG L   N+       ++    V++E K+ + ++++S+ +LL+        V  E G L      +P++  + +TRNLS+     +P+++++ R+ SI + +   DY +  LVL  N NE + + P   E+ +E + R   P+   S + R +    +         +++F F+ + + + L +G+               L +     LSV+G+   N  +  SV L NI + D + +S  +I                +E+I    N+ +  + +  I  K  ++   V + G  + +C+D+L+ LT F         +        PV    ++ +   +    LA+    PVE    +       + + +  P+I+LVE++ + +++ I+      L Y  + +  I+N  I   +   C F  E R+     I+ P  ISL  + P   GM  SL +  +IIN++PA I+ ++K M ++++ +  + +  + S   N       N W +  F    + F                 VE G D     L+ EQR       + DN ++ K  K  + +P I +++ES V     P++  +  +     N    +    +++++++Y+N  +  WEP++   EV      R+Y+ +E+++    + G+   + + ED    E  A+ + + S + LE+T+SK+ L +LS L +AF  A   +      I  APYV++N  G DV   + K
Sbjct:  973 TPKQENSSNYLFTVSCTIADKSSPEFSTKYNSTEQLVVANFEVLQIVLHQECLQRIMEVVNNFQRNLDLV------------LSSTRPRDRM----------------GSIGG--GDGI-----KRTLNVILEDTEEIMTTDQMKRRKKTRRTHVVETVKVRVIANLDQVGLVLTGRK-RPIAEMNVKKFVSSLIIKSSY--TEVNIGLKDIQVLDLNPYTIHKNILSIVGKDA-FNCQIVIYNK-EETQDYNSD----DMKITVDIGCMKIIFLNWFVAGVMNFLNNFTAAQATISQAGAAAAESARQKAMDAYET--ATRMKLNIRIKAPIIIVPIGSQDRNALLLDLGLLELTNNTVEVAVAEEERLAVIDEIKLQICDVKISKIVLLDGNESTVDEVDAEVGFLSKFNMMNPMSCTLSITRNLSYTWYRDVPELNLSGRLKSIELTLFADDYALVMLVLNRNLNEGLEEFPPSEEAPQEAQVR---PERRNSRAGRLSRTVQVSPIREKIHESIKFNFQFDGVVINLMEGEG------------AGLARFGIYFLSVKGTKLDNGTLSTSVVLCNIQMDDMRSNSKSQIRQYLSRKDWVQPKLDTDEIIDACYNERNFMVDVTAII-KEDDTFAEVRVRGFDLIVCIDFLLKLTTFLTLPPEENPRESVYIKPAPVSETARDTKHSIRSSAILAAQELVPVESSSHEVPNRKMNLILHIDEPDIILVENLEDLNTSCIIFNAQVHLNYRSINDKQIVNGQIDALKMYMCAFLPERREMTRHYILHPCVISLQGSTPEEEGMHISLKLSDIIINVSPATIELLNKAMLSVSSGTMTKCAIAEES--RN-----YSNLWHQHHFHSRTYWFTK---------------VEQGVDA----LEAEQRSV-----STDN-EKQKTEKCVIEIPSITLVIESGVGYYTKPLISLDTRITAVFNNWSRSLTAHGSLTLNMNYYNQALAEWEPIIELNEVIGRNGVREYTPWELKF----EMGMEKVQSELED--DAEQQAMHMNIHSAETLEITLSKTCLGLLSELAEAFSQAIDQNGLTKPDIV-APYVLENDTGFDVNLNLRK 1973          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. TrEMBL
Match: A0A5K4EAP5 (VPS13C protein, putative OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 2264.57 bits (5867), Expect = 0.000e+0
Identity = 1422/3696 (38.47%), Postives = 2136/3696 (57.79%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISK----------LTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETK-DSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSS--LASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDP------DIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTK--------GPDGSVPTLVSTQAQSKLMN-------------LLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMP-------------------GSGDTDL----------IFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQ-----TAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVL----PDSETASLTSTIXXXXXXXXXFKDAL--------------------EIMDNN---VXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTK-GQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEE-----KTETWSSPNKEETRKQTTLEK--ENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTSVV-------------LEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNE-------AINQVP-----SVPESKEEVKSRKILPKDDKSGSARTASDE--SITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKI--NELIRNIKNDSS------DFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPR------------KYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQ-STD------ETSTRDGSF----INNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDD--SGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLH-----LEQHKSIFGAPYVVKNTCGKDVVFYINKE------FD----------LKPCNSDEEFKVYLKADGEVQLYD-----------------AVDEKQSLITEEKQAKIVKF-----------GFADSISREYLSIYRTEVRALLFSPKD--NEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYL--LLEEVLGGKCYNVPIPTLLT-SNGKIYISVKQLNTEISNSGALW---NIG-KNAQPSDT---------------------------------QILLCKSNTGVM------YYYSVALSIEEVQ------------------------IVNSSETLRT-----------------YKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQSLDTVES--PAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKP-VTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVE---------NAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELI--LPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKV----EGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEIEADLIVLKAVVSKI 10136
            MVFESLVVELIN+YLG Y+  L+ SQLK+GLLGGNA LENLDIK NAFDD DLPVKV QGHISK          LTLKIP+K+L++EP IAEL GLYVLI+PN  ++YD  KE+ Y  E KQKEL  +E  +LK   PK   ++K DSFGEKLAAQ++KNLQISI +IHIRYED+ + PNR F++G+TL+ L+FQTCDS+   I IK++S R+F K VK+ SL VYWN     F+ + +  L++ M+++I+S +   T   +L++PIS +AM+H+H +PE TN++VP+M L +   +I   F+ +QY DIT FL++ DR  T+ ++RKYRP  P+  N  +WW F YN +LEE VRRR  MWSW++I  HR   R Y  IW +KL   KLSQ++   I+  EE LD+ NI LCRQQAE++    ++K +QK +S      +   S    + S  + S    +L K++ +E T EEKA L+  I YSE           + PI YVS  + + L++L  ++ D+ L +  ++K  ++ ++A V+QR+   A++V+L M++++  G +              P L+++     L N             LL +DFE NPLD+ A+Q+I+I+A+ L+I+YD++T+N IV F TPP+++ L E+S  VL+S EEVKE+T +GLRHLA  R YTD+ +DIKPSYFILP+ G Y  DC++LLVDLGS  + S RTS+P                    +G TD           +  K GKL +D +K+ AYDKF+I LS++Q +M+ +GDDWR LR    S NHIL+P      LK+CV+ ND NLP++ V GCLP   V  +D VL+S+ ELI+++PFP+     T P  P    + +L++       I P   L +  +L+    L     DS  +S  S   +DD  +E   D +                    +++D N    I  + + +   K   +IDL   FVI   E+QVL+   N+  +      I+   +HE+GT+L  +R+   +QA+IG+L +A+PQF    T  PV LA+T++H + G +LL + L+I EK +PDF T + NTRH+L+C FK ++V +HQEA L+++N+ N+L   L+   +E     + E  +S    E  KQ  L +  ENE  + L  +      +G     I   TE+R +R++A ++++++       GK   IPE+ +TQW + A+L K+ V + + K+ ++   +++G+R DV+T+Y T E+S  +++  + D      Y+KI+W++  E++ S+Q+  F   T +    +D  +VD  +++++GK+  IFLY+F+ R+  F+ ++QE++  ++NK Q  S+AA  ++   ++    +R  L+I  +APVIY+P++S S  +LM+D G +T  N F     + V+             LE   + L NLR+SR +L E++V+ EK +L PIN+ +   RNL+ N    IP + +   +  I++ +SQGDY + Q VL  NFN+       +I   P     S   + ++ K+++I+ + + S  +    +    IT+ F   M+ + + L+  D    EW ++ P DK L     +  +V G M  +S+ +   +L++IN+ D++  S K+I   +++R + + ++      + I ++F QD   N K+ + +  I++C  +DYLM L DF +K+ P         +  V    K  R            K    P A P      + E+ V++  PE++LVED+ N DSNAI + T     Y++ ++  +IN  I+      CP+        + +I+SP+ +S Y ++PIN  + G+L M+ L INI P  I+ +S I +++ +  +TD      ET + D S     +++ D+  +  FW+  +  E    ++      K+KI    + VE   D+A+++ + E RR D                I +R+  I++ILES++  K +PML+ + ++D  L  P+T + L   + +S+SY+ND IN WEPL+E  PEE +R +S ++E +S ++  +    ++D+E++  ++ S  +IL+LSRD LE+T+SK+AL++L+NLG++FE AY L       +   +   APY + N  G  ++   N +      FD          L   +S+ +  +    + ++  Y                    D+KQS+++  K ++   +           G + S S     +YR +   L  S KD  NE P+  +  +  I  ++    A LG+K +  RS +QV NNT + I +Y    KS    +  N+L  LL  V  G+ Y +P+  + +     I+I  +     IS + A W     G +   PS+T                                 Q++ C +N+G M      Y+Y+VA+     Q                        +   ++ LR                  +++ I   + L+NQLP  I++     +  I  G    ++TA     + I++++ Y    + G L+L P+MEE SVI F++++ Y    + LG+H     GQ  +T+Y+PYWM+N                                                 K++   + +S+D VE+  PA         T T ++   +     F K  KA+L++ +S WSDKFSLDTVGSSG+V C  K  ++YE+GVKI+LSSSGLTKIVTF+PY++I+N+++I + C E+ E         N GWIKVP GEAVPFWP +   K+M+++C+V   E +VT  FPFYESHSILMKL+ K+ GIFVEV+ TEE  +I LQ+YQEGMALV+L+N+L     + + Q    Q ++  L A  ++MYAW   + KRE I+       +HSN LT D IE+F +N  KAY VSF+W+  R+LLFT + +VAK+ARLS+DLE IDQEI ISL SIG+SLVDN  + ELAYISITSSGV+W+Q++R NR KPL+L  SE LE A+  +VN    ++ +V   IVK+    + +EVD ++ +++KP++  ++R F+PGVFLQY++S  Q+ +H +++++Q+D+Q  D  FPVV + FP P+SVALD+G KPL E SA+I ++   G+ R+KYF VLIQEMQL LDQGF+  I +F S SV    +E  F  D  +I + LI+   ++  +  G + +FD+FHISPIK+H+SFSL+       +A  SEVLNLFLQS+G+A+T++QDV+FKL YFE+Q  IM + QM + IT HYV Q +RQMYVLVLGLDVLGNPFGV+RG+AQGVEDLFYEPV+GA+LGPEEF EGV  GV+SL GHAVGGAAGAVSRI GT+GKGIAALTLD +Y+RKRREQLA+RPE  G GLAQGG+GL++G   GVTGVVTKP +GAK++G+EGFFKG GKGLVG VTRPVSGVVDFASSSFEGIRR+ADT  EV R+RPPRFI  DGIIR Y  REA+G  I  ++ +  D I  + Y+FH++     + + LT   +  ++  ++LG +SI W+   E L   P IN+S   I+  LKEKQ+KLF+  +  K+ + DL + K ++S+I
Sbjct:    1 MVFESLVVELINRYLGSYIEALNASQLKIGLLGGNAQLENLDIKANAFDDLDLPVKVLQGHISKQKLLHLLLGSLTLKIPFKNLFTEPTIAELTGLYVLIVPNIAVEYDEAKEKRYAKELKQKELVSVETARLK---PKHSDDSKADSFGEKLAAQVIKNLQISIKDIHIRYEDSFSIPNRTFSLGLTLSALTFQTCDSNQKPIFIKDESIRQFIKFVKLTSLGVYWNPMSTKFAEKAKVPLRSAMRSSIASPDNLNTGFHYLVKPISSTAMLHIHTRPEETNYSVPQMDLVVNFSEIELCFSLSQYHDITCFLEAQDRIVTQGKYRKYRPLTPISQNYKVWWRFAYNAVLEESVRRRHLMWSWEHISKHRKISREYIAIWRKKLSGDKLSQAQLANIEAYEEFLDVFNIVLCRQQAELQFNASKQKHSQKANSKSGGGWFGWFSRGSAAGSDDMESSSEGDLMKRLETEMTSEEKAHLFAVIDYSESAASGVSGSAINFPISYVSSSIAVTLRQLSLVLLDDKLSDPNVLKFSVNKLTADVKQRAGDQALAVSLRMDSLEALGAQPTLKESYLAKSSIKPVLITSHVNQSLSNGNNQAGNTSKRSHLLCIDFEKNPLDRKADQRISIQADALQIVYDAETINKIVHFFTPPKDVYLQELSNTVLSSFEEVKEVTTSGLRHLATNRSYTDVHIDIKPSYFILPDSGIYRSDCRLLLVDLGSFTVNSSRTSVPPVCKTVQDQASSNDENKNDCTGKTDAKEPISNISGQVKHKSGKLTFDELKDEAYDKFNINLSSVQVIMVNEGDDWRALRKMNKSSNHILKPLALSCTLKRCVLRNDVNLPRVIVDGCLPVFSVDFTDAVLKSLYELIVNVPFPEADKSLTTPANP----DELLKDVVVLPNSIIPGSVLATRDLLKVTRQLNRNDSDSTLSSWVSDTVDDDLHEESEHDRISQQSKLENDTTNVNDDLHGSQLIDKNKSKAIRQRDQANLRRKRAKLIDLKLDFVIRKVEIQVLERQLNSQNKNIEDHLILSFNIHEVGTSLIKRRWTQEIQAHIGTLSVAIPQFTHRETYEPVYLARTQRHEETGHHLLAVQLIIAEKNAPDFDTYYQNTRHRLQCDFKVLDVYVHQEATLLLMNYFNNLRISLSKQSKELEHSSEIEWATSIGDIEGLKQAKLRETLENEMKQQLPGRLTAADAVG---SHIARKTERRNIRMEAASLAQKT-------GKVAVIPELNITQWMVNATLDKVNVTLGS-KETDLFSTTVNGLRIDVRTTYDTVEISAILSEFIVIDHIVNTSYRKILWVDPTENVVSLQITHFQDNTQSIENSIDPDKVDLAISLQIGKLHSIFLYHFITRVLNFMEAYQEAVGIMSNKFQALSDAAVQQVHEAVDNSSQTRLSLNIVAQAPVIYMPQHSLSNVSLMIDFGRITLSNHFEFINPSKVLPDTTIPVPEPGIMLEHMVVRLENLRLSRVVLKESSVQAEKHVLLPINVELKTRRNLNPNIYSAIPILLVNGGLNEINISLSQGDYRVLQEVLLDNFNDLPSSTKTSIKTTPVTTDSSAKPTNKKNKTQRIVEEKNISTQSDNHDNNPVKITISFDLGMKSVILNLYTCDQPVAEWLNDFPEDKRLATFNLKQFNVSGQMASDSSSQFIAKLNDINLQDSRPGSDKQITRGQILRVLGHSATKDNRHDELIYVEFNQDALLNKKIQISLRSIHLCASMDYLMSLLDFQMKSTPTSKNESNN-LTSVTDKSKQKRLNNSSITKPDVKKTESLPTAYPF-----NLELKVQIDNPELVLVEDIYNVDSNAIKLTTELSFGYNMKQDIVVINALINSLSIAACPYNETVGGPFSKEILSPSDLSFYASQPINGSLHGTLHMDTLTININPNTIRLLSAISSSVQSSLTTDKNPDICETVSNDESMKSVNVDHSDEKSLTEFWKPIQLKELNMPYLGLTSDSKDKI----ECVEKACDIAKVVEETENRRID---------------IINIRLKTIRIILESQLDTKFMPMLVLDSTLDAVLTKPITHVELKFLIELSLSYYNDSINQWEPLIETLPEEGDRMWSLQLELVSINNNKAECLTDDDWENVGCLQASTNTILILSRDNLEITISKTALEMLNNLGRSFEAAYKLKNIKSKYDDSTNRIIAPYCIMNKTGCQLMLRYNSQALSLIHFDSDNTMTKSESLHQTSSEPKKAIVSNTEHDIVTYQNSVLLDSQQEIGFTELAKTDKKQSIVSSYKDSRHHIWISLSDTSHQPSGSSISNSEVVFQLYRGDSELLCLS-KDPSNEKPTNSD-RKSTIYPIVADVEACLGVKSITFRSTVQVLNNTSERICIYSHLIKS----SVANHLPGLLTIVDAGQSYALPVEIVNSRQQTGIFIGPEYEKPVISVTPAYWPEFACGVEKYTPSNTNTHLPFLAPYAQSVSSTNTEYKISNSSQFKWPNQLVECHTNSGSMNNNGIIYFYNVAVVNHNYQDNRLDNTNNDNNNTGNTDQQRKTSLTAVADELRATQKKMIQGDKLSITHCDFQMIIQTAVVLYNQLPFSINFILTDITDTIAAGSHYALKTASPN-NAYIEISFEYTGSMYRGKLELHPQMEEYSVIEFESRKEYEVFDLPLGLHRTSNLGQICLTLYAPYWMIN-------------------------------------------------KTEKDLTYKSVDDVETVHPA---------TFTGALLYSSLSKSFFGKR-KANLRVCESNWSDKFSLDTVGSSGRVHCTTKSRMSYEIGVKIDLSSSGLTKIVTFMPYFVIINKANINIDCCEVDETETKSTQSTNTGWIKVPAGEAVPFWPHATNSKNMLLKCRVS--ENIVTAAFPFYESHSILMKLDGKYPGIFVEVETTEETTVITLQMYQEGMALVRLVNNLDGSQPVFYHQSGI-QHIVHRLDAGMSIMYAWDCPQAKRELIFYCNETDNRHSNKLTYDSIEEFKVNNTKAYLVSFMWNMQRVLLFTQNANVAKNARLSSDLEPIDQEIVISLQSIGISLVDNHSRAELAYISITSSGVRWSQVKRGNRLKPLELTLSENLERAYTNYVNASNDTD-KVAFSIVKLTDTRQPIEVDFNRMIILKPDQYGITRAFDPGVFLQYKSSISQMQLHAKLFRLQVDSQRLDSTFPVVLSAFPQPKSVALDSGFKPLFEWSAIIGRTANPGSFRFKYFHVLIQEMQLKLDQGFLYDITNFFSSSVTRTSKEEAFKHDYLLITSKLIDLPVVQAHMQDGNRSIFDSFHISPIKVHVSFSLTGSTEGKSSAFPSEVLNLFLQSLGVALTEVQDVVFKLDYFERQACIMTLGQMSTEITHHYVGQGVRQMYVLVLGLDVLGNPFGVLRGLAQGVEDLFYEPVKGAILGPEEFAEGVTLGVRSLLGHAVGGAAGAVSRITGTLGKGIAALTLDEEYKRKRREQLARRPETFGAGLAQGGRGLVMGVFHGVTGVVTKPFEGAKREGVEGFFKGFGKGLVGAVTRPVSGVVDFASSSFEGIRRLADTTQEVSRVRPPRFIRSDGIIRPYDKREADGYLILKQVDK--DGIFGQ-YLFHIHSVRGNSALILTTNNLLIIESMDLLGTFSIDWKTPLENLSEAPVINKSG--ILIKLKEKQKKLFTSGHQIKQFDCDLNLAKLMISEI 3578          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. TrEMBL
Match: W6V289 (Vacuolar protein sorting-associated protein OS=Echinococcus granulosus OX=6210 GN=EGR_00351 PE=4 SV=1)

HSP 1 Score: 1934.46 bits (5010), Expect = 0.000e+0
Identity = 1249/3618 (34.52%), Postives = 2011/3618 (55.58%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMR--KKLNQKT---------XXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDG----------------------------------------------------SVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSI----------LIVSERTS------------MPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESIL----REYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIXXXXX--XXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQ------NLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLI------PVLNIVEEEKTETWSSPNK------EETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI---------DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKE-------------EVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDS---SDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDN----FWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKA----------FEDAYS-----LHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKV--------------------YLKADGEVQLYDAVDEKQSLITEEKQA-----KIVKFGFA----DSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVST-TAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGK---IYISVKQLNTEISNSGALW------NIGKNAQPSDTQILL------------------C----KSNTGV---MYYYSVALS----------IEEVQIVNSSETLR-TYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLK-VQSN---IKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVK-KSDSGYSLQSLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFA---------KTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKK-PVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG------WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLE----VDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSL-SRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLK-----EKQRKLFSKDNITKEIEA 10100
            MVFE+LV +L+N+YLG+Y+  L+ SQL+LGLL GN +LEN+D++  AFDDF++P +V QGHI    L     H Y           YV         Y+  KE+ Y  E K+K L  IEE K +R   K ++   DSF EKLAAQI+KN+Q+ I NIHIRYED +T P   F+ G+TL  L+FQTCDSSG  +++  D ++EFFK V++DSLAVYWN + K ++      L+ +M ++++SSER + ++ ++++PIS +A++HVHM+PE   FT+P++ +DLK E+I   F  NQY  +   LDS+DR   +N+F KY+  +  ++     W F Y  +LEE VRRR  MWSWK+IQ HR   R YT +W ++LL + L+  ++  I+  E+EL+++N+ L RQ AE++A K R  K+ +++                +      DT  ++ +  DG   +Q+ V+ E T EEK KLY A+ YSE  +    P EYVS  +   L  L  ++++N LK   ++K+ ++++++  +QR   +A+  + + +     G  GP G                                                    + P L+S+    +  +LL VDF  NP+D++A++ + I A PL++IYD+DTVN IV F   P  L+L EIS+ V+ S++E+K MT TGLRHL  RR   D++VDI+PS  +LPERG Y + C++++VD GS+          L  SE TS            + G   +D  + +  + NY+ +   AYD+F + LS  Q +++++G++WR+LRS   +  H+L+P G +  L++C    +  LP+  V G LP LG+  +D+VL+S+ ++I H+P P+T P + +PT  + L    + +  K   + P         L+A++VL  +ETA    + + D    E + F D+ E  +  +    + + +     N++ + A F++    + + +   +  +    A  ++K +++EIG   TL+++D  + A++  L L  P+F +  + + +CL  T   +  Q      +L   +LLI +K+ PDF T +ANTRH L C+F+ +++ LHQEAL+ +L F   L       P L  +E++  +     N+       E  + +++   +EA     +     ++ GL+  +     E+R +RL  +AVSE +       G+ + IPE+  TQW I  +L K++V   +  +  ++  S+ GMR D++ +Y TTE+++ ++D+ I D +  ++Y++I+WIE   ++F MQ  L+N+GT     + D  +VD  V ++ GK  +I LY F+ R+                            I  V+ +P   R  L +NV+APVIY+P+NS S  AL+ DLG ++ KN F +         D    ++++   + L + ++S +IL++  V+ ++ ++ PIN+ ++M R +  ++   +PQ+ ++  + SI + M+ GDY +   V   NF EA + + S+P S               E+ ++K     D S S    S    +  + F +  L++ELF+G  +P  W  E+   + L    C  L +EG    +++  +  RL +I+++DT+  S + I  ++    +     S+FI + +  D  SN  L V++  I +C  +DY M+L+ FF +      +P  K        ++      ++P  + V        I   +A PEI+L EDM NP+ NAI+V T+    Y++++E  ++N  I+ F+ + CPF    R KN+M+++ PTS+S Y  +P+   + GS+ ME L+IN+   +I+SISKIMN LT  + D +        +  ++ + D+    FW  +    ++ + I  G ++ +  ++ ++G E G DVA IL+ V+  R +            K S I +R+  + + +ES++  K VPML+ E  +DG++K+P T + + +   ++VSY+NDH+N WE LLE  P E NR +S ++ Y S +    + ++++++L +++ S   +  +S+D LE+T+SK+ALDVLS LGKA          FE+AY      + L +   I  APYV++N  G  ++  ++ +   +   +DE                         L+A   +  YD    K   +   + A     + +  G A    D I+     ++ +   AL      N   S  +++++E +  IV+T TA+LG K L L+S LQV N T + I ++ L   +  S+      LL  + GG  Y+  +P  + +N +   I +         S     W         K+    DT I L                  C    K+  G    +Y Y++ +            + +Q  +S+ T    Y + I  P +LHN LP+ I++        ++PG      TA L  V+S+   + V + Y KK++ G++++  + EE+S++  + ++GY  S ++LG+  + + G  ++T+Y PYW++NKTG +LTYK  ++ +  H A  +D  LFS            T   A+++ K  S   LQ + + +      H++    + AS ++ +N ++ F          + VK  ++  DSLWS KFSLDTVGS  +V C+ K   +YE+GVKINLS+SGLTKI+TF+PY+M++N++S+ + C + V + G      WI +  G+  PFWP +   + M+M C+V+ +  ++T  FP YES+++L+KL   ++G++ +VQ T+EA +INLQLY+EGMALV +IN +     +HF Q  T     L +   + Y W D  + R+F   V+  +K     +T+D  E F       + VSF+    R LLFTND  VA     S +LES+   I++ L S+G+SLV N L++E+ YISI SS + WN++RR NR KPLK   SEALE  ++ F+NR+  +E +  + +   + +     VDL++ +M++PE C L R FEPG+ LQYR SP+Q+ +H +I+++Q+D+Q  D +FPVVFAPFP P SV +D+G K L+E+  VI K+ +    R+K    LIQEMQ  LDQGF+N ++DF +GSV   DE   F+ D ++   +L++   +K +L  G++ + D  HISPIK+H+SFSL      +  +ALHS+VL LFLQS+G+A+TD+QDV+FKLGYFE++  IM  +++++ +  +Y  QA++Q YVL+LGLDV+GNPFGVIRGMAQGVEDLFYEPV+GAV+G EEF EGVA GV+SLFGH VGGAAGA+SRI GT+GKG+AALTLD +Y+R+RRE++ + P+N G G+A+GGKGL++G V GVTG++T+PI GA+K+G+EGFFKG+GKGL+G VTRPVSG+VDFASSSFEGIRR   T+ +V  +RPPRFI  DG+IR Y   EAEG+ I  ++ ++    P+E YVFH+  +  K +  LT +RI  +  NEILG  +I WQ    +L      ++S+H   + L+       + KLF   +  K+IE 
Sbjct:    1 MVFETLVADLLNRYLGNYLETLNASQLRLGLLSGNVSLENVDVRATAFDDFEIPARVVQGHIRTFFL-----HTYC----------YV--------AYNEAKEKQYRREAKRKTLEAIEELKQERQSEKGDS--PDSFFEKLAAQIIKNVQVKIENIHIRYEDQTTIPGVRFSTGLTLNRLAFQTCDSSGEPVVLSSDLSKEFFKLVELDSLAVYWNHDTKMYAGLPTGLLRNVMSSSVASSERTVVEIDYILRPISFNALLHVHMQPEKVQFTIPQVGVDLKFEEIEVEFQHNQYVGLVLLLDSVDRLMLRNKFWKYKSLIESKNYPRARWLFAYTAVLEEIVRRRSNMWSWKHIQEHRQMVREYTALWRKRLLKESLTPEQEGKIEYFEDELNVMNLALARQHAEVQAAKSRAAKESSRRGGGGWFSWFLGGSSSAASATATGDT-LATCVRGDGPQVIQR-VQQEMTPEEKEKLYAALNYSEGMSRCTYPPEYVSTAVKFSLGGLAIMLANNDLKNPGILKVQLNTVTSDFKQRVGDNAMEEDSV-DKCCSFGRPGPGGPNLHDKDAVSLKSLPRGFFGRYCCQFVLLCPCSFSVRLAGFEVVGPRNSSTDTAPILISSAVTGE--SLLSVDFAKNPIDRTADRCLFIAAAPLDVIYDADTVNKIVDFFKLPPGLRLDEISSSVMPSLDEMKAMTTTGLRHLLSRRTCLDLRVDIQPSCLLLPERGVYIEGCRLIMVDFGSVSLRSMLAHQDLARSETTSDQVSVASRASSALVGRAQSDERYKQLLQANYEQLLFEAYDQFEVTLSATQIILVQKGENWRELRSLADTPGHVLKPLGIKLVLRQCTAPLEFGLPQFQVDGRLPLLGIDFTDKVLESLCDIIAHVPLPETDPSQRIPTDPNALLKDVKFFDTKTEDLLPGQ-------LKAVHVLRMAETALAPPSPESDQADDEADVFFDSYETPEACISSAVQSSVRKPLA-NLVMICASFLVEEIRICMFEQTTSGDISDPPAP-MLKFVINEIGADFTLRKWDQEMNAHVNRLSLKAPRFKEYGSGNELCLIDTFSRSDDQTTPSSEHLFTAHLLIADKSGPDFATKYANTRHCLVCEFRVLKIHLHQEALVSLLGFFTDLAKVLAKDPELGAIEDKNQKALEWANRLGDTEASEHLQTSSVMTPHEARLSAAKTTPVDAVSGLLARK----NERRDIRL--DAVSEAAKM-----GRYLRIPEVDATQWKIQLTLDKVQVFFCS-SEMQLMAFSMRGMRVDLRMTYLTTEIAMALSDICISDLNETSLYRQILWIEPGANVFLMQTVLYNRGTKALDDQYDPEKVDVAVCLQFGKAHVIMLYWFLRRV---------------------------VIQEVVNQPLPPRLALSVNVEAPVIYMPQNSTSRTALVFDLGRISIKNHFEVIPKPVLSKNDPINMLMMDCMSVRLEDFKMSAAILVDGGVEIDRNVIQPINLSLNMNRKVFSSKGDGVPQLDVSGALESIRLSMTYGDYKLINEVFLDNFEEADSLMGSLPISNPTNVATSEDPVIVTELTTQK---AADVSTSDSLDSASEKSASWTFVLRRLEIELFRGTDDPRNWNDEIHASRRLGVFTCTELFLEGDSFDDTSSNMMARLKDISLIDTRPHSDEHITNILSQAGDSQGQMSNFIEVLYHSDNQSNQTLNVNVCSIMVCASLDYFMILSKFFTEGAHHRNRPAAKPSP--PPSQRTSVMKHRQPQRTTVSQNPGTLRIKTNIADPEIVLPEDMSNPNCNAILVTTSLNFDYAMLREETVMNASITKFRILACPFDRSQRDKNSMEVVVPTSVSFYARQPVAAALHGSINMESLLINVNAPIIRSISKIMNQLTESAMDVSLVPQTVGGSQHEKTLGDDGTEDFWSIKPVKASD-IPIPMGQIDYD--DSASEGTE-GCDVADILMKVDDERVE------------KASSILIRIGYVCLSMESQIGNKQVPMLLMEAHVDGEMKSPSTVLEVSLNFELTVSYYNDHVNEWEQLLETLPTEDNRLWSLQVGYHSAEQEFFYTKKEWQELSTLQNSGRLLTFISQDNLEITISKTALDVLSKLGKASFPRPIPGASFEEAYVSPRPVIPLRESIEI-KAPYVLRNQTGLRLMVVVDGQ---RLRTTDESISAKSASSLSLGAAQQKIGLVGHVLEAGQNLGFYDVHMNKIPTVQYLRSAPLAPPRFINIGLAPKSVDLIND--WGVFASRTAALKIHRTANYIVSAVSINDKEKSQPIVATITAFLGQKTLFLQSTLQVVNETPEAIRIFSLLSPT-FSDAVAQIQLLAIIAGGGSYS--LPVEIVTNKEITGIRMCPDLEGAAPSTDVIYWPPKVCPQTAKSENRGDTCIYLLPRLDSGRESEWPVQEVGCSVPLKAEAGAGKALYNYTITMERRFPEGQDGHPDFLQPPSSTTTEPVAYNMVIRAPASLHNHLPVPITFYLCDAISTVEPG-----HTAVLSSVKSSGFSVSVNFTYNKKDYLGAINISNRTEEMSLLPCETQDGYDISLLNLGLRCSRRLGHIDLTVYCPYWLVNKTGLSLTYKDQNELEFHHPAGFSDVFLFSV-----------TSKTALLQHKPSSKLHLQPVSSTD------HENAASDAVASTSSEHNCSKPFHSMHRGIRLIQKVKIAMRTEDSLWSSKFSLDTVGSWDRVHCEGKLGRSYEIGVKINLSASGLTKIITFMPYFMVVNKTSVAVECAQ-VRDIGKNILDTWIPIKAGDVKPFWPATGDTQKMLMTCRVDQK--VITDYFPLYESNTVLLKLPNPYIGLYTDVQTTDEATVINLQLYKEGMALVHIINDMDGGRNVHFYQKGTGVTHTLKSGYQMFYTWDDASKPRQFFTFVDNSQKTRQVEITTDSYETFVSGNANIHVVSFLSKLQRTLLFTNDPTVANLTAKSGELESVTDRISLVLQSVGISLVTNQLRKEVCYISIRSSDIIWNKMRRGNRLKPLKPDVSEALEDVYNTFLNRVAQNE-DFPKRVTLPDTIRPTTTVDLNQMVMLEPENCTLQRTFEPGMQLQYRASPNQIQIHAKIFRVQVDSQRLDAHFPVVFAPFPQPTSVVVDSGSKALVEVFTVICKTTDEEVYRFKRLECLIQEMQFQLDQGFLNDMIDFFAGSVIIQDELVGFLADQKLTSGALVDAPVVKDVLQTGRESIIDYIHISPIKMHVSFSLLGSSQESQPSALHSDVLKLFLQSLGVALTDVQDVVFKLGYFERRGRIMSFSKILTEMGRYYAGQAIKQTYVLILGLDVIGNPFGVIRGMAQGVEDLFYEPVKGAVVGTEEFAEGVALGVRSLFGHTVGGAAGAMSRITGTLGKGVAALTLDDEYKRRRREEMTRTPDNFGAGMARGGKGLVMGVVGGVTGIITQPILGARKEGVEGFFKGLGKGLIGTVTRPVSGMVDFASSSFEGIRRATSTSKDVLPVRPPRFIHLDGVIRPYDRNEAEGSIILSRLRDRS---PSEDYVFHIPLRRNKGICMLTTERILLVTMNEILGSCTIDWQAKLSQL------SDSQHKYEYGLELQLGNASKTKLFGGASNVKQIEC 3475          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. TrEMBL
Match: A0A068Y6L9 (Vacuolar protein sorting associated protein 13A OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000774600 PE=4 SV=1)

HSP 1 Score: 1719.13 bits (4451), Expect = 0.000e+0
Identity = 1120/3290 (34.04%), Postives = 1833/3290 (55.71%), Query Frame = 3
Query:  819 MKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRP-----NVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMR------KKLNQKTXXXXXXXXXXXXXDTETSSSLAS----DGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPD-GSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSIL---------------------IVSERTSMP-GSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESIL----REYSGKQLGIAPKLNLTSNQVLRAINVLPDSETA--SLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIV-------KLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQ------NLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLI------PVLNIVEEEKTETWSSPNK------EETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI---------DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKE-------------EVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDS---SDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVD----NFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAY-----SLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKV------------------YLKADGEVQLYDAVDEKQSLITEEKQA-----KIVKFGFA----DSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVST-TAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALW-------NIGKNAQPSDTQI-LLCKSNTG------------------------VMYYYSVALS----------IEEVQIVNSSETLR-TYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACL-KVQS---NIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQ-SLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKK-PVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG------WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLE----VDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFS-LSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLK-----EKQRKLFSKDNITKEIEA 10100
            M+P    FT+P++ +DLK ++I   F  NQY  +   LDS+DR   +N+F KY+      N P R++ S W  F Y  +LEE VRRR  MWSWK+IQ HR   R YT +W ++LL + L+  ++  ++  E+EL+++N+ L RQ AE++A K R      ++      S          S T T  +LA+    DG   +Q+ V+ E T EEK KLY A+ YSE  +    P EYVS  +   L  L  ++++N LK   ++K+ ++++++  +QR   +A+  ++ +   +V G +     + P L+S+    +  +LL VDF  NP+D++A++ + I A PL++IYD+DTVN IV F   P  L+L EIS+ V+ S++E+K MT  GLRHL  RR   D+++DI+PS F+LPERG Y + C++++VD GS+                      + S  +S P G   +D  + +  + NY+ +   AYD+F + LST Q +++++G++WR+LRS   +  H+L+P G +  L++C+   +  LP+  V G LP LG+  +D+VL+S+ ++I H+P P+T P + +PT  + L    + +  K   + P         L+A++VL  +ETA    +   D+ D+E + F D+ E  +  +    + + +     N++ + A F++        ++I+  + E+  + +IV       K +++ IG   TL+++D  + A++  L L  P+F +  + + +CL  T   +  Q      +L   +LLI +K+ PDF T +ANTRH L C+F+ +++ LHQEAL+ +L F   L       P L  +E++  +     N+       E  + +++   +EA   + +     ++ GL+  +     E+R +RL  +AVSE +       G+ + IPE+  TQW I  +L K++V   +  +  ++  S+ GMR D++ +Y TTE+++ ++D+ I D +  ++Y++I+WIE   ++F MQ  L+N+GT     + D  +VD  V+++ GK  +I LY F+ R+  F++SF+++ +    KA E + AA  +I  V+ +P   R  L +NV+APVIY+P+NS S  AL+ DLG ++ KN F +         D    ++++   + L + ++S +IL++  V+ ++ ++ PIN+ ++M R +  ++   +PQ+ ++  + SI + M+ GDY +   V   NF EA + + S+P S               E+ ++K     D S S    S    +  + F +  L++ELF+G  +P  W  E+   + L    C  L +EG    +++  +  RL +I+++DT+  S + I  ++    +     S+FI + +  D  SN  L V++  I +C  +DY M+L+ FF +      +P  K        ++      ++P  + V    +   I   +A PEI+L EDM NP+ NAI+V T+    Y++++E  ++N  I+ F+ + CPF    R KN+M+++ PTS+S Y  +P+   + GS+ ME L+IN+   +I+SIS+IMN LT  + D +        +  ++ + D    +FW  +    ++ + I  G ++ +  ++ ++G E G DVA IL+ V+  R +               KI +R+  + + +ES++  K VPML+ E  +DG++K+P T + + +   ++VSY+NDH+N WE LLE  P E NR +S ++EY S +    + ++++++L +++ S   +  +S+D LE+T+SK+ALDVLS LGK+FE+AY      + L +   I  APYV++N  G  ++  ++ +  L+  +     K                    L+A   +  YD    K   +   + A     + +  G A    D I+     I+ +    L      N   S  +++  E +  IV+T TA+LG K L L+S LQV N T   I ++ L   +  S+   +  LL  + GG  Y++P+  +             L     ++  ++          K+    DT I LL + ++G                        V+Y Y++ +            + +Q  +S+ T    Y + I  P +LHN LP+ I++        ++PG      TA L  V+S   ++ V + Y KK++ G++++  + EE+S++  + ++GY  S ++LG+  + + G  ++T+Y PYW++NKTG +LTYK  ++ +  H A  +D  LFS  SK+   +      + +++  DS +S + SLDTV S                                         W            G+V C+ K   +YE+GVKINLS+SGLTKI+TF+PY+MI+N++ + + C + V + G      WI +  G+  PFWP +   + M+M C+V+ +   +T  FP YES+++L+KL   ++G++ +VQ T+EA +INLQLY+EGMALV +IN +     +HF Q  T     L +   + Y W D  + R+F   V+  +K     +T+D  E F       + VSF+    R LLFTND  VA     S +LES+   I++ L S+G+SLV N L++E+ YISI SS + WN++RR NR KPLK   SEALE  ++ F+NR+  +E +  + +   + +     VDL++ +M++PE C L R FEPG+ +QYR SP+Q+ +H +I+++Q+D+Q  D +FPVVFAPFP P SV +D+G K L+E+  VI K+ +    R+K    LIQEMQ  LDQGF+N ++DF +GSV   DE   F+ D ++   +L++   +K +L  G++ + D  HISPIK+H+SFS L     +  +ALHS+VL LFLQS+G+A+TD+QDV+FKLGYFE++  IM  +++++ +  +Y  QA++Q YVL+LGLDV+GNPFGVIRGMAQGVEDLFYEPV+GAV+G EEF EGVA GVKSLFGH VGGAAGA+SRI GT+GKG+AALTLD +Y+R+RRE++ + P+N G G+A+GGKGL++G V GVTG++T+PI GA+K+G+EGFFKG+GKGL+G VTRPVSG+VDFASSSFEGIRR   T+ +V  +RPPRFI  DG+IR Y   EAEG+ I  ++ ++    P+E YVFH+  +  K +  LT +RI  +  NEILG  +I WQ    +L      ++S+H     L+       + KLF   +  K+IE 
Sbjct:    1 MQPGKVQFTIPQVGVDLKFDEIEVEFQHNQYVGLVLLLDSVDRLVLRNKFWKYKSLIESKNYP-RASTSRWL-FAYTAVLEEIVRRRSNMWSWKHIQEHRQMVREYTALWRKRLLKESLTPEQEAKLEYFEDELNVMNLALARQHAEVQAAKSRAAKESSRRGGGGWFSWFLGGSSSAASATTTGDTLATCVRVDGPQMIQR-VQQEMTPEEKEKLYAALNYSEGMSRCTYPPEYVSTAVKFSLGGLAIMLANNDLKNPGILKVQLNTVTSDFKQRVGDNAMDFSVRLAGFEVVGPRNSSTDTAPILISSAVTGE--SLLSVDFAKNPIDRTADRCLFIAAAPLDVIYDADTVNKIVDFFKLPPGLRLDEISSSVMPSLDEMKAMTTAGLRHLLSRRTCLDLRIDIQPSCFLLPERGVYIEGCRLIMVDFGSVSLRSMLAHQDLARSETTSDQVSVASRASSAPVGRAQSDERYMQLLQANYEQLLLEAYDQFEVTLSTTQIILVQKGENWRELRSLADTPGHVLKPLGIKLVLRQCIAPLEFGLPQFQVDGRLPLLGIDFTDKVLESLCDIIAHVPLPETDPSQRIPTDPNALLKDVKFFDVKTEDLLPGQ-------LKAVHVLRMAETALAPPSPEFDQADDEADVFFDSYETPEACISSAVQSSVRKPLA-NLVMICASFLV--------EEIRICMFEQATSGDIVDPPAPMLKFVINGIGADFTLRKWDQEMNAHVNRLSLKAPRFKEYGSGNELCLIDTFSRSHDQTTPSSEHLFTAHLLIADKSGPDFATKYANTRHCLVCEFRVLKIHLHQEALVSLLGFFTDLAKVLAKDPELGAIEDKNQKALEWANRLGDTEASEHLQTSSVMTPHEARLSVAKTTPVDAVSGLLARK----NERRDIRL--DAVSEAAKM-----GRYLRIPEVDATQWKIQLTLDKVQVFFCS-SEMQLMAFSMRGMRVDLRMTYLTTEIAMALSDICISDLNETSLYRQILWIEPGANVFLMQTVLYNRGTKALDDQYDPEKVDVAVSLQFGKAHVIMLYWFLRRVVCFMDSFKDATEYAIEKASEVTGAAKTQIQEVVNQPLPPRLALSVNVEAPVIYMPQNSTSRTALVFDLGRISIKNHFEVIPKPVLSKNDPINMLMMDCMSVRLEDFKMSAAILVDGGVEIDRNVIQPINLSLNMNRKVFSSKGDGVPQLDVSGALESIRLSMTYGDYKLINEVFLDNFEEADSLMGSLPISNPTNVATSEDPVVVTELTTQKTA---DVSTSDSLGSASEKSAFWTFVLRRLEIELFRGTDDPRNWNDEIHASRRLGVFTCTELFLEGDSFDDTSSNMMARLKDISLIDTRPHSDEHITNILSQAGDSQGQMSNFIEVLYHSDNRSNQTLNVNVCSIMVCASLDYFMILSKFFTEGAHHRNRPAAKPS--PPPSQRTSTMKHRQPQRTTVSQNPSTLRIKTNIADPEIVLPEDMSNPNCNAILVTTSLNFDYAMLREETVMNASITKFRILACPFDRSQRGKNSMEVVVPTSVSFYARQPVAAALHGSINMESLLINVNAPIIRSISRIMNQLTESAMDVSLVPQTVGGSQHEKTLGDDGTEDFWSIKPVKASD-IPIPMGQVDYD--DSASEGTE-GCDVAGILMKVDDERVE---------------KILIRINYVCLSMESQIGNKQVPMLLMEAHIDGEMKSPSTALGVSLYFELAVSYYNDHVNEWEQLLETLPTEGNRLWSLQVEYHSAEQESSYTKKEWQELSTLQNSGRLLTFISQDNLEITISKTALDVLSKLGKSFEEAYVSPRPVIPLRESVEI-KAPYVLRNQTGLRLMVVVDGQ-RLRTTDKSISAKSASSLSLGAAQQKIGLVGHVLEAGQNLGFYDVHMNKIPTVQYLRSAPLAPPRFINIGLAPKSVDLIND--WGIFASRTAGLKIHRTANYIVSAVSINAEEKSQPIVATITAFLGQKTLFLQSTLQVVNETPKAIRIFSLLPLT-LSDAVAHTQLLAIIAGGGSYSLPVEIVTNKEITGIRMCPDLEGAAPSTDVIYWPPKVCPQTAKSENRGDTCIYLLPRLDSGRESEWPVQEVGCSVPLKAEAGAGKVLYNYTITMERRFPKGQDGHPDFLQPPSSTTTEPVAYNMVIRAPASLHNHLPVPITFYLCDAISTVEPG-----HTAVLSSVKSSGFSVSVNFTYNKKDYLGAINISNRTEEMSLLPCETQDGYDISLLNLGLRCSRRLGHIDLTVYCPYWLVNKTGLSLTYKDQNELEFHHPAGFSDVFLFSVTSKTALLQ-----HKIVMRTEDSLWSSKFSLDTVGS-----------------------------------------W------------GRVHCEGKLGRSYEIGVKINLSASGLTKIITFMPYFMIVNKTPVAVECAQ-VRDIGKNILDTWIPIKAGDVKPFWPATGDTQKMLMTCRVDQKA--ITDYFPLYESNTVLLKLPNPYIGLYTDVQTTDEATVINLQLYKEGMALVHIINDMDGGRNVHFYQKGTGVTHTLKSGYQMFYTWDDASKPRQFFTFVDSSQKTRQVEITTDSYETFVSGNANIHVVSFLSKLQRTLLFTNDPIVANLTAKSGELESVTDRISLVLQSVGISLVTNQLRKEVCYISIRSSDIIWNKMRRGNRLKPLKPDVSEALEDVYNTFLNRVAQNE-DFPKRVTLPDTIRPTTTVDLNQMVMLEPENCTLQRTFEPGMQIQYRASPNQIQIHAKIFRVQVDSQRLDAHFPVVFAPFPQPASVVVDSGSKALVEVFTVICKTTDEEVYRFKRLECLIQEMQFQLDQGFLNDMIDFFAGSVIIQDELVGFLADQKLTSGALVDAPVVKDVLQTGRESIIDYIHISPIKMHVSFSLLGSSQESQPSALHSDVLKLFLQSLGVALTDVQDVVFKLGYFERRGRIMSFSKILTEMGRYYAGQAIKQTYVLILGLDVIGNPFGVIRGMAQGVEDLFYEPVKGAVVGTEEFAEGVALGVKSLFGHTVGGAAGAMSRITGTLGKGVAALTLDDEYKRRRREEMIRTPDNFGAGMARGGKGLVMGVVGGVTGIITQPILGARKEGVEGFFKGLGKGLIGTVTRPVSGMVDFASSSFEGIRRATSTSKDVLPVRPPRFIHLDGVIRPYDRNEAEGSIILSRLRDRS---PSEDYVFHIPLRRNKGICMLTTERILLVTMNEILGSCTIDWQAKLSQL------SDSQHKYECGLELQLGNASKTKLFGGTSNVKQIEC 3152          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. TrEMBL
Match: A0A068WCF9 (Vacuolar protein sorting associated protein 13A OS=Echinococcus granulosus OX=6210 GN=EgrG_000774600 PE=4 SV=1)

HSP 1 Score: 1707.96 bits (4422), Expect = 0.000e+0
Identity = 1122/3287 (34.13%), Postives = 1829/3287 (55.64%), Query Frame = 3
Query:  819 MKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRP-----NVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMR--KKLNQKT---------XXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPD-GSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSI----------LIVSERTS------------MPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESIL----REYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIXXXXX--XXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQ------NLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLI------PVLNIVEEEKTETWSSPNK------EETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI---------DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKE-------------EVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDS---SDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDN----FWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYS-----LHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKV--------------------YLKADGEVQLYDAVDEKQSLITEEKQA-----KIVKFGFA----DSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVST-TAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGK---IYISVKQLNTEISNSGALW------NIGKNAQPSDTQILL------------------C----KSNTGV---MYYYSVALS----------IEEVQIVNSSETLR-TYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLK-VQSN---IKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQ-SLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG------WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLE----VDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFS-LSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLK-----EKQRKLFSKDNITKEIEA 10100
            M+PE   FT+P++ +DLK E+I   F  NQY  +   LDS+DR   +N+F KY+      N P R++ S W  F Y  +LEE VRRR  MWSWK+IQ HR   R YT +W ++LL + L+  ++  I+  E+EL+++N+ L RQ AE++A K R  K+ +++                +      DT  ++ +  DG   +Q+ V+ E T EEK KLY A+ YSE  +    P EYVS  +   L  L  ++++N LK   ++K+ ++++++  +QR   +A+  ++ +   +V G +     + P L+S+    +  +LL VDF  NP+D++A++ + I A PL++IYD+DTVN IV F   P  L+L EIS+ V+ S++E+K MT TGLRHL  RR   D++VDI+PS  +LPERG Y + C++++VD GS+          L  SE TS            + G   +D  + +  + NY+ +   AYD+F + LS  Q +++++G++WR+LRS   +  H+L+P G +  L++C    +  LP+  V G LP LG+  +D+VL+S+ ++I H+P P+T P + +PT  + L    + +  K   + P         L+A++VL  +ETA    + + D    E + F D+ E  +  +    + + +     N++ + A F++    + + +   +  +    A  ++K +++EIG   TL+++D  + A++  L L  P+F +  + + +CL  T   +  Q      +L   +LLI +K+ PDF T +ANTRH L C+F+ +++ LHQEAL+ +L F   L       P L  +E++  +     N+       E  + +++   +EA     +     ++ GL+  +     E+R +RL  +AVSE +       G+ + IPE+  TQW I  +L K++V   +  +  ++  S+ GMR D++ +Y TTE+++ ++D+ I D +  ++Y++I+WIE   ++F MQ  L+N+GT     + D  +VD  V ++ GK  +I LY F+ R+ +F++SF+ + +    KA E + AA  +I  V+ +P   R  L +NV+APVIY+P+NS S  AL+ DLG ++ KN F +         D    ++++   + L + ++S +IL++  V+ ++ ++ PIN+ ++M R +  ++   +PQ+ ++  + SI + M+ GDY +   V   NF EA + + S+P S               E+ ++K     D S S    S    +  + F +  L++ELF+G  +P  W  E+   + L    C  L +EG    +++  +  RL +I+++DT+  S + I  ++    +     S+FI + +  D  SN  L V++  I +C  +DY M+L+ FF +      +P  K        ++      ++P  + V        I   +A PEI+L EDM NP+ NAI+V T+    Y++++E  ++N  I+ F+ + CPF    R KN+M+++ PTS+S Y  +P+   + GS+ ME L+IN+   +I+SISKIMN LT  + D +        +  ++ + D+    FW  +    ++ + I  G ++ +  ++ ++G E G DVA IL+ V+  R +               KI +R+  + + +ES++  K VPML+ E  +DG++K+P T + + +   ++VSY+NDH+N WE LLE  P E NR +S ++ Y S +    + ++++++L +++ S   +  +S+D LE+T+SK+ALDVLS LGK+FE+AY      + L +   I  APYV++N  G  ++  ++ +   +   +DE                         L+A   +  YD    K   +   + A     + +  G A    D I+     ++ +   AL      N   S  +++++E +  IV+T TA+LG K L L+S LQV N T + I ++ L   +     A+  LL   + GG  Y+  +P  + +N +   I +         S     W         K+    DT I L                  C    K+  G    +Y Y++ +            + +Q  +S+ T    Y + I  P +LHN LP+ I++        ++PG      TA L  V+S+   + V + Y KK++ G++++  + EE+S++  + ++GY  S ++LG+  + + G  ++T+Y PYW++NKTG +LTYK  ++ +  H A  +D  LFS  SK+   +      +  ++  DS +S + SLDTV S                                         W      D V   GK+       +YE+GVKINLS+SGLTKI+TF+PY+M++N++S+ + C + V + G      WI +  G+  PFWP +   + M+M C+V+ +  ++T  FP YES+++L+KL   ++G++ +VQ T+EA +INLQLY+EGMALV +IN +     +HF Q  T     L +   + Y W D  + R+F   V+  +K     +T+D  E F       + VSF+    R LLFTND  VA     S +LES+   I++ L S+G+SLV N L++E+ YISI SS + WN++RR NR KPLK   SEALE  ++ F+NR+  +E +  + +   + +     VDL++ +M++PE C L R FEPG+ LQYR SP+Q+ +H +I+++Q+D+Q  D +FPVVFAPFP P SV +D+G K L+E+  VI K+ +    R+K    LIQEMQ  LDQGF+N ++DF +GSV   DE   F+ D ++   +L++   +K +L  G++ + D  HISPIK+H+SFS L     +  +ALHS+VL LFLQS+G+A+TD+QDV+FKLGYFE++  IM  +++++ +  +Y  QA++Q YVL+LGLDV+GNPFGVIRGMAQGVEDLFYEPV+GAV+G EEF EGVA GV+SLFGH VGGAAGA+SRI GT+GKG+AALTLD +Y+R+RRE++ + P+N G G+A+GGKGL++G V GVTG++T+PI GA+K+G+EGFFKG+GKGL+G VTRPVSG+VDFASSSFEGIRR   T+ +V  +RPPRFI  DG+IR Y   EAEG+ I  ++ ++    P+E YVFH+  +  K +  LT +RI  +  NEILG  +I WQ    +L      ++S+H   + L+       + KLF   +  K+IE 
Sbjct:    1 MQPEKVQFTIPQVGVDLKFEEIEVEFQHNQYVGLVLLLDSVDRLMLRNKFWKYKSLIESKNYP-RASTSRWL-FAYTAVLEEIVRRRSNMWSWKHIQEHRQMVREYTALWRKRLLKESLTPEQEGKIEYFEDELNVMNLALARQHAEVQAAKSRAAKESSRRGGGGWFSWFLGGSSSAASATATGDT-LATCVRGDGPQVIQR-VQQEMTPEEKEKLYAALNYSEGMSRCTYPPEYVSTAVKFSLGGLAIMLANNDLKNPGILKVQLNTVTSDFKQRVGDNAMDFSVRLAGFEVVGPRNSSTDTAPILISSAVTGE--SLLSVDFAKNPIDRTADRCLFIAAAPLDVIYDADTVNKIVDFFKLPPGLRLDEISSSVMPSLDEMKAMTTTGLRHLLSRRTCLDLRVDIQPSCLLLPERGVYIEGCRLIMVDFGSVSLRSMLAHQDLARSETTSDQVSVASRASSALVGRAQSDERYKQLLQANYEQLLFEAYDQFEVTLSATQIILVQKGENWRELRSLADTPGHVLKPLGIKLVLRQCTAPLEFGLPQFQVDGRLPLLGIDFTDKVLESLCDIIAHVPLPETDPSQRIPTDPNALLKDVKFFDTKTEDLLPGQ-------LKAVHVLRMAETALAPPSPESDQADDEADVFFDSYETPEACISSAVQSSVRKPLA-NLVMICASFLVEEIRICMFEQTTSGDISDPPAP-MLKFVINEIGADFTLRKWDQEMNAHVNRLSLKAPRFKEYGSGNELCLIDTFSRSDDQTTPSSEHLFTAHLLIADKSGPDFATKYANTRHCLVCEFRVLKIHLHQEALVSLLGFFTDLAKVLAKDPELGAIEDKNQKALEWANRLGDTEASEHLQTSSVMTPHEARLSAAKTTPVDAVSGLLARK----NERRDIRL--DAVSEAAKM-----GRYLRIPEVDATQWKIQLTLDKVQVFFCS-SEMQLMAFSMRGMRVDLRMTYLTTEIAMALSDICISDLNETSLYRQILWIEPGANVFLMQTVLYNRGTKALDDQYDPEKVDVAVCLQFGKAHVIMLYWFLRRVVSFMDSFKNATEYAIEKASEVTGAAKTQIQEVVNQPLPPRLALSVNVEAPVIYMPQNSTSRTALVFDLGRISIKNHFEVIPKPVLSKNDPINMLMMDCMSVRLEDFKMSAAILVDGGVEIDRNVIQPINLSLNMNRKVFSSKGDGVPQLDVSGALESIRLSMTYGDYKLINEVFLDNFEEADSLMGSLPISNPTNVATSEDPVIVTELTTQK---AADVSTSDSLDSASEKSASWTFVLRRLEIELFRGTDDPRNWNDEIHASRRLGVFTCTELFLEGDSFDDTSSNMMARLKDISLIDTRPHSDEHITNILSQAGDSQGQMSNFIEVLYHSDNQSNQTLNVNVCSIMVCASLDYFMILSKFFTEGAHHRNRPAAKPSP--PPSQRTSVMKHRQPQRTTVSQNPGTLRIKTNIADPEIVLPEDMSNPNCNAILVTTSLNFDYAMLREETVMNASITKFRILACPFDRSQRDKNSMEVVVPTSVSFYARQPVAAALHGSINMESLLINVNAPIIRSISKIMNQLTESAMDVSLVPQTVGGSQHEKTLGDDGTEDFWSIKPVKASD-IPIPMGQIDYD--DSASEGTE-GCDVADILMKVDDERVE---------------KILIRIGYVCLSMESQIGNKQVPMLLMEAHVDGEMKSPSTVLEVSLNFELTVSYYNDHVNEWEQLLETLPTEDNRLWSLQVGYHSAEQEFFYTKKEWQELSTLQNSGRLLTFISQDNLEITISKTALDVLSKLGKSFEEAYVSPRPVIPLRESIEI-KAPYVLRNQTGLRLMVVVDGQ---RLRTTDESISAKSASSLSLGAAQQKIGLVGHVLEAGQNLGFYDVHMNKIPTVQYLRSAPLAPPRFINIGLAPKSVDLIND--WGVFASRTAALKIHRTANYIVSAVSINDKEKSQPIVATITAFLGQKTLFLQSTLQVVNETPEAIRIFSLLSPTFSDAVAQTQLL-AIIAGGGSYS--LPVEIVTNKEITGIRMCPDLEGAAPSTDVIYWPPKVCPQTAKSENRGDTCIYLLPRLDSGRESEWPVQEVGCSVPLKAEAGAGKALYNYTITMERRFPEGQDGHPDFLQPPSSTTTEPVAYNMVIRAPASLHNHLPVPITFYLCDAISTVEPG-----HTAVLSSVKSSGFSVSVNFTYNKKDYLGAINISNRTEEMSLLPCETQDGYDISLLNLGLRCSRRLGHIDLTVYCPYWLVNKTGLSLTYKDQNELEFHHPAGFSDVFLFSVTSKTALLQ-----HKIAMRTEDSLWSSKFSLDTVGS-----------------------------------------W------DRVHCEGKL-----GRSYEIGVKINLSASGLTKIITFMPYFMVVNKTSVAVECAQ-VRDIGKNILDTWIPIKAGDVKPFWPATGDTQKMLMTCRVDQK--VITDYFPLYESNTVLLKLPNPYIGLYTDVQTTDEATVINLQLYKEGMALVHIINDMDGGRNVHFYQKGTGVTHTLKSGYQMFYTWDDASKPRQFFTFVDNSQKTRQVEITTDSYETFVSGNANIHVVSFLSKLQRTLLFTNDPTVANLTAKSGELESVTDRISLVLQSVGISLVTNQLRKEVCYISIRSSDIIWNKMRRGNRLKPLKPDVSEALEDVYNTFLNRVAQNE-DFPKRVTLPDTIRPTTTVDLNQMVMLEPENCTLQRTFEPGMQLQYRASPNQIQIHAKIFRVQVDSQRLDAHFPVVFAPFPQPTSVVVDSGSKALVEVFTVICKTTDEEVYRFKRLECLIQEMQFQLDQGFLNDMIDFFAGSVIIQDELVGFLADQKLTSGALVDAPVVKDVLQTGRESIIDYIHISPIKMHVSFSLLGSSQESQPSALHSDVLKLFLQSLGVALTDVQDVVFKLGYFERRGRIMSFSKILTEMGRYYAGQAIKQTYVLILGLDVIGNPFGVIRGMAQGVEDLFYEPVKGAVVGTEEFAEGVALGVRSLFGHTVGGAAGAMSRITGTLGKGVAALTLDDEYKRRRREEMTRTPDNFGAGMARGGKGLVMGVVGGVTGIITQPILGARKEGVEGFFKGLGKGLIGTVTRPVSGMVDFASSSFEGIRRATSTSKDVLPVRPPRFIHLDGVIRPYDRNEAEGSIILSRLRDRS---PSEDYVFHIPLRRNKGICMLTTERILLVTMNEILGSCTIDWQAKLSQL------SDSQHKYEYGLELQLGNASKTKLFGGASNVKQIEC 3152          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. TrEMBL
Match: V4C0Y2 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_160876 PE=4 SV=1)

HSP 1 Score: 1682.15 bits (4355), Expect = 0.000e+0
Identity = 1183/3463 (34.16%), Postives = 1869/3463 (53.97%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIK----------------------------------------ENAFD--------------DFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEE-NKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMN--LLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTK-GQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQ---ESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTS-----VVLEESKITLSNLRVSRSILLEN-TVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEA--INQVPSVPE-SKEEVKSRKILPKDDKSGSARTASDES--------ITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIR------NIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLE-VYPEEHNRQYSFEIEYISCDDSGL-FDEEDFE-DLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSL-HLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYD------AVDEKQSLITEEKQA--KIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEI--SNSGALWNIGKNAQPSDTQILLCKSNTG-VMYYYSVALSIEEVQIVNSSE-TLRTYKLDINVPLALHNQLPIKISYQSQIFSHD--IKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGD-DCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQSLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKI-NDSLWSDKFSLDTVGSSGKVMCKKKPVT--YEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVE----NAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYIN-EMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEG-LEVDLSKK---LMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSL---SRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFL-TNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQ 10055
            MVFESLVV+LIN++LGDYV  LD SQLKLG+ GG+A L+NLD+K                                        E+ +D              D DLPVK++ GHI KLTLKIPWK+LY+ PV+AE+DG+Y L +PN+ ++Y+ EKE+    + KQK+L +IEE  KL+ +  K +   +DSF EKLA Q++KN+QI +SNIH+RYED  T PN  FA+G TL +L FQT D + T  +IK D+  + +K +++D L+VYWNS+ + F    +  +   +K++++ +E K+  + ++++PIS  A + ++ KPE +NF++P++ L L  + +  A N+ QY D+   L+S++R      +RKY+P+VP + ++  WWH+ Y  ILEE VRRRR+MWSWK I  HR   + Y + + +KL  KK+S S+Q  +   E+ LD+ +IT+ RQQAE++A K+  K  ++   G +   + G    ++    A+D   E++ K    +T EEKAKLY AIGYSE   DP  P E+V+++L  +L  L   ++D + ++  +M+L +  +   V QR +A+A+ +   ++NM VTGT   +   P LV +Q Q K  N  LL V+FETNPLD   + +I + + PLEI YD+ T+N +  F  PPE + L ++ST  +A  +E+KE +  GL+H  D+R YT+I V++  SY ++P  G      K LL+DLG++ + +++         D         N + + + AYDKF+I L  IQ L IE  D+W+K R    S  H+L P      L KC+   D N+PK+ + G LP+L + ISD  LQ ++ L+  IPFP++ PP+P                          + + +  N LP       T+ I              E  DN V   +K TS+     N  DL+  F I   E++V + ++N  +       ++KLIV  +GT ++++ FDM V AY+G + +   +F    +   + L  T    K   +LL +  L  +K  P+F + + +T   +   F ++EV LHQ A+L ++ F   L P              S NK          K   AIED                         + + +     ++ D N     KK   P++   Q  + A L    V +    D  + D+ I G+ A V      T V   + D+ I DP P  +Y KI+ IE  E +  ++V ++N GT    Y  D+S VD  +++ +G IK++FL  +V  +  FL++FQ   E +Q       EYS+  A    ++ E  P  R  ++IN+KAP+I +P+ S S N LM+DLG L   N F +    S      VL++ KI L  L++SR+ +L N  +K E  IL P++I +D+ RNLS    H  P++ I+  + +I V +SQGD+ M   ++  N  E   I++  ++P  SK   K+ +I P+     SA    +ES          ++F F+++ L    + GD++ +    +   D  L K   + +++ GSM  +++M   ++L++  + D ++   K +  +I       N K    + I + F Q+   +  + VD+  + +C+CV++LM L DFF+K    P  P +         EK   K +++ + +PVE    +  I   + RPEI+L+ED LNPD+ ++++ T    K     +   +   I       C +    R+   + I+ P  I+L  + P        + +  +I+NI+PA I++++ + +  + Q ++   +       NQ ++I  + W  ++  E+   F    L +K +        ES  D +   L  E  R +Q               + + +  + + LE  V K+ VP+LI E S  G++KN  +Q+ +  ++S+ V+Y+N+ + VWEPLLE V  +    ++   +  ++ DDS +  D+E+ + D I + P  ++I + S D L +T++K+ L+VL  LGK+F DAY+L      K    +PYV KN  G D++  ++  F   P NS    +V L +   +  Y+      ++  K S+I   +Q   K + F      +   L I R E R    + + + G         ++ +++  T   +G K + LRS++Q+ NN    + + Y  K   K ET            G+ ++VP+  + + +G++Y   K L+ E   S +G  W   KNA   D + + CK+  G   +Y++V  ++E++    S++ + + Y L+++  + LHN LP+ + YQ +  + +  ++ G    +  A +   +      +Y+ ++W G+  L   + ELSV   +A EG +   MDLG+H  I NG  +VTI+SPYWM+NKTG TL Y+  D D  +EH       +LF                          Y  +SL + +   E   D               K ++  ++ KA +KI ND+ WSDKFSLDTVGSSG V CK K     YEVGV I+++SSGL+KIVTF P+Y++LN +  V+ C E+ E    +  W  +PP  ++PFWP  +V K + ++ +V +  V+ T PF   ++H+ LMKLE +F GI VE  ++E A +   + Y+ G A V ++N     V +H+ Q        L + ++ +Y W+D   K E IW   G +K   N LT DGI +F+++ + K YWVSF+    R+LLFT D+ +A  A+ + +LE IDQEI +S+  +G SLV+N  Q E+A++ ITSSG+ W +  +K R+K L L  +  LE A+ ++++ L    +      +++E  +EVD S K   +M+KP K E+ R F  G++LQY+ S HQV  H ++ ++Q DNQ++   FP V +P PPP+SVA ++  KP IE+S +  K   T   + KY +VLIQEM + +DQGF+N++L+  +   P   E+  ++  C +   S+  +              F++      KIHLSFSL   S  +    T + S+V+N+FLQSVG+ +TD+QDV+FKLGYFE+ +     +Q+ + +T HY  QA++QMYVLVLGLDVLGNPFG++RG+++GV DLFYEP +G + GPEEF EG+  GV+SLFGHA+GGAAGAVSRI GT+GKG+AALTLD DYQ+KRR+QL K+P +   G A+GGKGL++GFV G  G+V KP++GA+K+G+ GFFKGVGKGLVGVVTRP SGV+DFASSS EGI+R+ D + EV R+RPPR    D IIR Y  +EAEG  I ++ TEK     ++ Y  HV     K ++FL T+ R+  + + E+ G+W  +W   +++L+G P   ++   +   LKEK+
Sbjct:    1 MVFESLVVDLINRFLGDYVENLDSSQLKLGIWGGDAVLQNLDVKESALSQIRSISFPSVFECPVEEFAATPDGILHPTQLQFAQESLYDVRDVSEYTELKLVNDLDLPVKIKAGHIGKLTLKIPWKNLYTAPVVAEIDGIYALAVPNASIRYNEEKEQKAKQDAKQKKLKQIEEAKKLEADKDKAKDPKQDSFAEKLATQVIKNIQIKVSNIHVRYEDNLTNPNHPFAIGATLKDLLFQTTDENWTPTVIK-DAVTQIYKLLRLDMLSVYWNSSDRMFQDLPKEAILPALKSSLNPAENKIP-LQYILKPISSVAHLKLNTKPELSNFSIPKIFLTLVFDNVAIALNKLQYNDVLETLESLERMALLGIYRKYKPDVPFKGHVKKWWHYAYESILEETVRRRRKMWSWKNIIKHRKLMKSYREAYVRKLDTKKVSSSDQKILDEGEKLLDVFSITIMRQQAEVEAAKLGAKRKEEKGGGWFGGWFGGGKKGKSEVETAAD---EIKDKFYEMYTPEEKAKLYGAIGYSENEADPTFPKEFVAVQLVTKLNNLSVSLNDTTKEKPLIMRLSLKDVFTSVGQRPAANAVKLEAKIDNMTVTGTMQ-NNIRPKLVLSQTQDKDQNYSLLTVNFETNPLDGGCDTRIRVNSRPLEIAYDAITINQLADFFKPPESVYLKQLSTAAMAKFDEIKEQSTAGLQHAIDQRKYTEINVNLMSSYVMIPHGGFMKSGVKCLLLDLGNLKVSTQKNQSVDLKKAD---------NIENLMKKAYDKFNITLERIQLLYIEPDDNWQKARDAGISPLHLLDPMSIIIELHKCMFDKDPNMPKMKIFGELPNLSLNISDYRLQQLIALVDSIPFPES-PPQPA-----------------------IEDDLFQGPNTLPMVTLEPSTTVI----------NKVTEQSDNKVKKLQKGTSQEYT--NYTDLELKFEIKEIEVKVHEKLENKDIP------LLKLIVKSVGTVVSMRSFDMVVDAYLGDISVQHLKFQALKSGPVINLVNTNVDGKQSTHLLKVQYLKADKKGPEFTSTYKSTEQAISVNFMALEVLLHQAAILNLMEFAQKLQPP------------PSDNK----------KPALAIED-------------------------SKKEEKNKEKKKVDENKPKRQKKKVNPDLIDIQ--VNAKLDLFSVAICTN-DKVLTDVKIKGIDASVTIQKAKTSVGAVLQDITIFDPMPKTLYPKIMSIEGGE-VLKLEVVIYNDGTEGKHYG-DMSCVDTQLDVNIGCIKLVFLNRYVADLLRFLDNFQIAKERVQEAGEVMAEYSKEVA---ASLQERAP--RVGMNINMKAPLIIVPQKSTSNNLLMMDLGNLAINNKFHLAGQVSTNGVPAVLDKMKIVLKELKLSRAQVLNNGQIKAECLILDPVSITIDICRNLSVAWYHGHPEVDISGILETIDVKLSQGDFAMIMKLVDENLGEGTDISEQQALPAPSKPTPKTNEIQPQTSTLVSAEPKKEESQKPGDKAYCKMKFDFQLKNLSTTFYNGDSDMSHGIVKRKSDDLLGKFALQGMALNGSMMSDTSMVTKIKLTDTILDDLRIAKKKGLTRMITRSTRGGNDKKPCINMIDVDFTQNSTQDKNITVDVCSLKICVCVEFLMTLGDFFMKGMVKPPPPSRDLPP-PDVKEKQLVK-KQEAIDAPVE---GEMNIVFTMERPEIILIEDQLNPDTTSLILDTEIFFKMRQTPDVQTMQAAIKGLHIKSCSY--SKRELPGIKILPPCDINLVSSAPHGKDHHMDVNITDIILNISPATIRTMAAVASGFSVQPSENEKS-------NQ-EVIPADLWATKKVTEDSHWFTK--LPQKPE--------ESEDDRSGTPLKGEGDRCEQ---------------MIISLSSLVIKLEGGVGKRTVPLLIVESSFQGEVKNWSSQLYVDTSLSLEVAYYNELLAVWEPLLEPVVKDSKVHRWDLNLNVVAADDSIIPVDQEETDVDNIVLPPPKMTINVKSVDNLNITMTKTCLEVLGKLGKSFNDAYNLVKPGAQKDEITSPYVFKNQTGLDLLLKLDTTFQ-SPDNS-LSGRVKLASGETLPTYEKPGSVMSMSPKASVIKATQQGVEKKIIFQVEQFGATRELVIKRAEKRLFQINQRSHSG---------DMWSIVCCTDTNVGQKIVSLRSIVQICNNLTMPVEILY--KGDTKLETCG------VAYEGEIFSVPLAAVYSLHGELYF--KPLDGEYMDSENGYKW---KNA--GDMKQICCKNAPGQPSFYFNVNPTVEDIYFEASNDKSSKCYMLNLHPTVILHNLLPMSVKYQLEGSADETTLEDGVNVPLHHASVNDSTLALTLPNYRGRDWTGNRVLKLDVPELSVWTIEAYEGSTRITMDLGLHCKINNGSFDVTIFSPYWMINKTGMTLYYQGSDSDESIEHPPIFDGCILF-------------------------AYKPKSLFSKKKKTEKSDDK------------KTKVKEMKQSGKAQMKIGNDTEWSDKFSLDTVGSSGSVQCKNKSTGRLYEVGVNISITSSGLSKIVTFSPFYLLLNTADYVIQCAEVQEKSTQDVTWSDIPPQGSIPFWPEQDV-KEVKLKARVSNT-VINTTPFLVNKAHTTLMKLENEFGGIQVECNVSESAMVTKFKSYKSGFATVNIVNFTSKGV-VHYNQSGHSLIHTLNSEESCLYTWEDALGKLELIWSC-GKEKDMKNELTQDGIGEFFLDSDTKIYWVSFLDGMQRVLLFTEDLVLATLAQQAGELERIDQEINLSIVGMGFSLVNNYTQTEVAFMGITSSGIIWEE--KKKRYKGLNLKTTTILETAWQKYISELAVGHSPPSR--LQLENKIEVDFSNKDKLMMLKPNKREIKRSFIDGIWLQYKVSSHQVQFHAKLNRLQFDNQLNQAIFPTVLSPLPPPKSVAAESVPKPFIEVSMMQRKHEYTTVSQIKYAKVLIQEMAVHVDQGFLNAVLELFAADTPVSREQEFYIF-CLVCFLSVNHS--------------FEDPRAFLRKIHLSFSLQSGSSGDDGKPTEIKSDVVNVFLQSVGVVLTDVQDVVFKLGYFERNHKFYNQSQLTNEMTRHYAGQAIKQMYVLVLGLDVLGNPFGLLRGLSEGVGDLFYEPYQGLIQGPEEFAEGMVLGVRSLFGHAIGGAAGAVSRITGTVGKGLAALTLDDDYQKKRRQQLNKKPASAKEGFARGGKGLVMGFVDGAAGIVKKPLEGAQKEGVGGFFKGVGKGLVGVVTRPTSGVIDFASSSLEGIKRITDMSDEVRRLRPPRRFHNDQIIRPYNHQEAEGYAI-LQETEKGRFASSDEYSAHVVVSKDKKIVFLVTDLRLLLVKKGELFGVWDCEWSFEWKELNGTP--KKTSKGLEIILKEKE 3220          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Cavefish
Match: vps13a (vacuolar protein sorting 13 homolog A [Source:ZFIN;Acc:ZDB-GENE-030926-1])

HSP 1 Score: 745.732 bits (1924), Expect = 0.000e+0
Identity = 681/2302 (29.58%), Postives = 1099/2302 (47.74%), Query Frame = 3
Query: 3441 ITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTS-----VVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPES-KEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKN--DSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLA----SPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLT--AQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSF-------EIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFG---APYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSIS-REYLSIYRTEVRALLFSPKDNEGPS--------RGNVSEREINT-----LIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPT------LLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQI-LLCKSNTGVMYYYSVALSI--EEVQIVNSSETL-RTYKLDINVPLALHNQLPIKISYQSQIFSH----DIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAK------EG-YSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQSLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSS-IVLYCNELVENAGWIKVPPGEA-VPFWPRSEVGKSMMMR---CKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDD-VEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWN-QIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVE-GLEVDLSKK----LMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFV----SGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTG-KTALHSEV-----LNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKN---VIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEI 10094
            I A L  L+V +  EK   I +ISI G        +Y   VSI                        D+ +F  ++  +   T    Y +D+  VD  V + VG I++IFL  FV  +  F+N+FQE+ + +A    + +E AA  +  + E+  S+R  LD+++KAPV+++P++S S N ++ DLG +T KN F I  S S      +L+   + LS+L++ R+   +   + E  +L P+N+ + + RNLS +  H++P I I A +  +S+ +SQ D  +    L  N +E  + VP+   S K E  S K     +  GS  T    +  V+ G K                             K+K EN         N +M  SV+L+   +LD +    K I   +  ++   + +  + + + Q +   +   V +  +Y+C  +++L+ + D F+KA               +G+   P+  ++   A    +P +P  +  E+++ V  PEI+ V D+   D+ A+++ T   L       S  +   I+D + V CPF  + +  N   ++ P  +     +         L++  L + ++P +I ++  I + LT  A++ +E  +            +  + WE+R++ + +  F+ +            +G E    V  ++              G++L         LR+  I + LE+ V  + VPML+ + S  GD+KN  T I L   +++ V+Y+N+ + VWEPLLE  P E + +  F       +++    + SG+ DE D+  ++    + ISI   S+D L +T++K  L++LSNLG AF +A     +Q    F    AP+VVKN  G  V    ++ F  KP     + +     DGE     +++   +L T    A   +F    S+S + Y+         + FSP  +   S        RG  S   +++      I   ++  G K +++RS  Q  N+      ++                      G  C  V +P       L +   ++ +       E       ++  + +Q  D  +   C+ ++   + + V +    + V  +N+ E   + Y L +   + L N LP  I+Y            +K G    + TA +   S       Y +++W     L    +E++ I F++       +G  S + +D+ VH+  + GQA V ++SP        Y +  K G   Q + D                           I +K    Y +  L                         + K + F +  K  L I+DS  SD FS+DTVGS G V CK +   Y VGVKI+ SS  LT++VT LP+YM++N++  ++  C E  +   W +  PGEA VPFWP  E  K M ++   C+     +   QP      + +L+ L+    GI V+V +TE + +I    Y +G A   LINH  D  +E +   +   +E + P  + V Y W +    R   W       +  N    D ++D   +E K + +SF     R++LFT +  + K    S  +E  +QEI+++L  +G+SLV+NS  QE+AYI ITSS V W  + ++K+R K L    +E LE  +  +V       + V+  ++ +E G +V L+       M++P    L R F P + ++Y  S  Q    ++I ++QI NQ+    FP VF P  PP+SV +D+  KP  ++S +   +G +  +R KYF+VLIQEM L +D GF+ +IL+      +G+V +  E  +F KD + ++  L   S   T        +++ FHISPIK+HLSFSLS     G KT   +E+     LNL L+S+G  +TD+QDV+FKL +FE         Q+   +  HY  QA++QMYVLVLGLDVLGNPFG+IRG+++GVE  FYEP +GA+ GPEEFVEG+A GVK+L G AVGG AGA SRI G M KG+AA+T+D +YQ+KRRE + K+P  L  GL +GGKGL+ GFV G+TG+VTKPI GA+K+G  GFFKGVGKGLVG VTRP  G++D ASS+F+GI+R A+T+ +V  +RPPRFI +DG+IR Y  RE  G+ +  KI             +  +  +K N    + +T + IF + +    G  + +WQ  +++    P I E +  +    KE+ + +F      K I
Sbjct: 1077 IRADLSCLKVFIRGEK-ARISEISIEG------KLHYIIAVSIA-----------------------DKEVFDFRMVNYVDATEGDAY-MDMQIVDTSVTLTVGCIQVIFLNKFVSSVLAFINNFQEAKEALAEVTVQAAEKAATGVLELAEK--STRISLDVDIKAPVVFLPQSSISTNVIVADLGLVTVKNHFEIVPSPSHTKIPPILDVMSVALSDLKMYRTTFKDEKFQTEIQLLKPVNMDLSIKRNLSASWYHSLPDIQINAHLKPMSLILSQEDMIVVLRTLSENLSETSDAVPAPKGSDKAETSSTK-----ESQGSGGTTVVTAAVVE-GQKA---------------------------AKLKIENE--------NDSMTASVKLAEC-LLDDKRKIVKMITSRMMAMRKGAEQNTMVDVNYRQGREGTTLETV-VQDLYLCASMEFLLTVADIFMKAM-------------AQGFSSPPKSNKQTSTATKDAAPAQPAASKMEMNIVVKNPEIVFVADLTRADAPALVMTTQCELLMKSDPNSQQMTAAITDLKVVACPFVHKQQDSNITTVLQPCQVFFTSKQSPTQPQAIELSINSLTLKVSPIIINTVITIQSALTPAAETPEELESP-----------VPPDLWERRDWKDLKLWFLEE------------EGEEDTQSVTPLI------------PQGESLQ--------LRISSICLTLEAGVGHRTVPMLLAKSSFHGDVKNWSTLINLHCHLNMEVNYYNEIMGVWEPLLE--PLEDDTKDGFRPWTLDLKMKKKQANRSGISDEVDY--IVPDYKTVISIT--SKDQLNITLTKCGLNMLSNLGTAFAEA----AKQTAECFQKDQAPFVVKNRLGLPVSVLYSEVF--KPIGVQSKGRTVELQDGE-----SLNMDYTLTT----ADADQFSAMTSLSSKNYIQ------PTVYFSPVGHTTASVIPLIKVGRGMYSVMHLDSGVTRFFICQISSNEGSKFVKIRSPFQFINHFSVPFRVFE---------------------GSTCLGVSLPNEEFCVPLGSYKAQLSLQPAAEGDEQYECSESFSYDEVSQKPDECVEKTCRHHSSDAHVFKVNMVAVRDTVTSLNAQEEFDKPYVLHLWPTVLLRNLLPYPITYSLLAIGSPTPTQLKEGHSSQLHTAVMDHSSLELKLLGYLQQDWVAHYHLRNDHDEINFITFRSALQDDDVDGESSRAELDIAVHVKKEAGQAVVAVHSP--------YWMVNKTGRLLQYKADD--------------------------IHRKHPKDYDMPLL------------------------FSFKPRNFLQNNKVRLMISDSELSDDFSIDTVGSYGDVKCKGRAKDYLVGVKIDASSFSLTRLVTVLPFYMLVNRTKHVIRVCEEGQDE--WTEAQPGEAAVPFWPERE-SKKMKVKVDGCQTAAHTINFHQP-----ENCLLLHLDNTLGGIIVDVNLTEHSAVIRFSDYHDGAAPFLLINHTKDKTLEFYQSSHQQEKEELAPG-RAVYYTWAEPTGSRVLRWSWGPYSAELKNE--EDLLKDVN-DEGKLFVISFYEGLQRVVLFTTERRIYKLLCESERVELAEQEISLALQDMGISLVNNSTSQEIAYIGITSSDVVWEIKPKKKSRWKVLNGKDAEMLEQHYKSYVE-----SSPVDHSLLDLENGYQVCLTPNSVDMRMLQPYDAPLRRNFLPALKVEYSVSARQKAYRVQINRVQIQNQLPGAIFPFVFYPIKPPKSVRMDSEPKPFTDVSIITRAAGHSDILRIKYFKVLIQEMDLRMDLGFLYAILELFTAENAGTVTSEQEVELFEKDIEYLKTGLNPVSATDT----SPISLYEYFHISPIKLHLSFSLSSGGEDGNKTKRETEMIPVQSLNLLLKSIGATLTDVQDVVFKLAFFELTFQFRTTQQLQWEVIRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFVEGMALGVKALVGGAVGGLAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLKEGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVTRPTGGIIDMASSTFQGIKRAAETSQDVESLRPPRFIHEDGVIRPYKEREGFGSQMLQKIEN------GRFAKYRYFAHAKVNDSDFLMITKRGIFFVTKG-TFGQLTCEWQYLFDEFTKDPMIVEDRR-LRIEAKERVKSVFHAKEFGKII 3111          

HSP 2 Score: 582.793 bits (1501), Expect = 1.096e-166
Identity = 364/1056 (34.47%), Postives = 589/1056 (55.78%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLK---RNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPET-TNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKM-------RKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGS-DELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSE-RTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTE---SILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINS 3143
            MVFESLVV+++N++LGDYVV LD SQL LG+ GG+A L NL+IKENA    D+P KVR GHI +L LKIPWK+LY++ V A LD +Y+LI+P + +KYD EKE   L E +Q+EL RIEE K K   + +PK  TE +D+F EKL  Q++KNLQ+ ISNIH+RYED  T PN   + G++L NLS QT D++    ++ E + + FFK V++D+L  YWN N + +S+         ++ N++       D +F+ +PIS +A +   M P +  +F+ P++ L + L ++    +R QY  I   L S+D       +RKYRP VP+ +N   WW +  + +LE  V+ R  MWSW++I+ HR   + Y ++++ K+  KK ++     ++  E+ LD+ NITL RQQAEM+A K          K+ ++ S G +   W    D  T +  A  G  D L        T  EKAKLY AIGYSE   +P++P  +  +K+  +L  +   I + S ++ ++++L +  +SA + QR SA AI +   ++  +VTG    + +   L S +   +   LL + FETNPLD  A+Q++++ ++PLEIIYD+ TVN +  F  PP +LQL E++      +E+ ++ T+TGL ++ + +   D+ +++  SY I+P+ G Y     + ++DLG   + S+ + ++P      L+ G       D+M   AYD F I LS++Q L  +   DW+K R +  S  HIL P   +    + ++  D+ +PK  + G LP L + ISD  ++ VLELI  IP P++ PP     T+    +L++    QL   P+    ++  LR+ +++ DSE             +   F+ +   ++ +  + ++   K    KNM +    F I+   +Q+L+      +       ++ L +  +GT L L+ FDM+   ++  + L   ++ D   +  V L  T  +T+  +LL +  +  +K +PDF + + NT   ++  F S+++ LH EALL  +NF+NS
Sbjct:    1 MVFESLVVDVLNRFLGDYVVNLDSSQLNLGIWGGDAILRNLEIKENALSQLDIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDEVYLLIVPTASIKYDAEKEEKQLQEARQRELQRIEETKQKAAEQENPK--TEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPNCPLSFGVSLQNLSLQTADANWKPCLLDEKA-KLFFKLVRLDNLFAYWNVNSELYSNHTPPEALRRLQQNVAVQNSIPHDYNFIFRPISANAKL--RMNPRSDVDFSSPKVDLVVNLPEVAVELSRPQYVSILELLGSVDMMSRNLPYRKYRPCVPVHNNAYNWWRYVISSVLEVNVKPRLHMWSWQHIRRHRQLAKQYKELYKTKITSKKPTEVVLKKLEDTEKTLDIFNITLARQQAEMEASKAGLRIYRPGVKMEEEQSQGWFGWMWNWSGDGSTPAKEAKPGGFDTL-------MTPAEKAKLYTAIGYSETAVNPNLPKSFEDMKVRFQLVSMSVSIKE-SREKPEIIRLSVIGLSATLTQRPSAQAIKIIAQLSTFEVTGLPRSNVAPMLLSSRRVVEENKPLLSLMFETNPLDAKADQRLHVESQPLEIIYDATTVNSLSAFFMPPNDLQLDELTNATRMKLEQFRDRTSTGLMYIIETQKVLDLNINLMASYVIVPQMGFYDPSHNLAILDLGHFQMSSQSKANLP-----QLLAG--SSTIEDIMSR-AYDSFDIQLSSLQFLYTKPDGDWKKARKERQSALHILEPVDLKVVFSRAMVVTDSRMPKFKMSGELPLLSLRISDDKVRGVLELIDSIPLPESRPPPRAAATQVTPKVLKQRDTPQL--TPRRPAMTD--LRS-SIIFDSEEDLFFDAPSSPIEDLPFFEASPGALERSASVKRRSMVKQDPKKNMTEFLMNFEISKLSIQLLR------LSAGQEVPVLHLEIEGLGTELKLRTFDMSSYTFLREICLECSEYQDGDGRK-VHLITTLDNTE-DDLLTLEYVKADKNAPDFKSAYKNTEQLIKVNFSSLDIHLHTEALLNSMNFLNS 1022          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Cavefish
Match: vps13a (vacuolar protein sorting 13 homolog A [Source:ZFIN;Acc:ZDB-GENE-030926-1])

HSP 1 Score: 582.408 bits (1500), Expect = 1.225e-166
Identity = 364/1056 (34.47%), Postives = 589/1056 (55.78%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLK---RNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPET-TNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKM-------RKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGS-DELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSE-RTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTE---SILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINS 3143
            MVFESLVV+++N++LGDYVV LD SQL LG+ GG+A L NL+IKENA    D+P KVR GHI +L LKIPWK+LY++ V A LD +Y+LI+P + +KYD EKE   L E +Q+EL RIEE K K   + +PK  TE +D+F EKL  Q++KNLQ+ ISNIH+RYED  T PN   + G++L NLS QT D++    ++ E + + FFK V++D+L  YWN N + +S+         ++ N++       D +F+ +PIS +A +   M P +  +F+ P++ L + L ++    +R QY  I   L S+D       +RKYRP VP+ +N   WW +  + +LE  V+ R  MWSW++I+ HR   + Y ++++ K+  KK ++     ++  E+ LD+ NITL RQQAEM+A K          K+ ++ S G +   W    D  T +  A  G  D L        T  EKAKLY AIGYSE   +P++P  +  +K+  +L  +   I + S ++ ++++L +  +SA + QR SA AI +   ++  +VTG    + +   L S +   +   LL + FETNPLD  A+Q++++ ++PLEIIYD+ TVN +  F  PP +LQL E++      +E+ ++ T+TGL ++ + +   D+ +++  SY I+P+ G Y     + ++DLG   + S+ + ++P      L+ G       D+M   AYD F I LS++Q L  +   DW+K R +  S  HIL P   +    + ++  D+ +PK  + G LP L + ISD  ++ VLELI  IP P++ PP     T+    +L++    QL   P+    ++  LR+ +++ DSE             +   F+ +   ++ +  + ++   K    KNM +    F I+   +Q+L+      +       ++ L +  +GT L L+ FDM+   ++  + L   ++ D   +  V L  T  +T+  +LL +  +  +K +PDF + + NT   ++  F S+++ LH EALL  +NF+NS
Sbjct:    1 MVFESLVVDVLNRFLGDYVVNLDSSQLNLGIWGGDAILRNLEIKENALSQLDIPFKVRAGHIGRLELKIPWKNLYTQSVEATLDEVYLLIVPTASIKYDAEKEEKQLQEARQRELQRIEETKQKAAEQENPK--TEKQDTFVEKLVTQVIKNLQVKISNIHVRYEDDVTNPNCPLSFGVSLQNLSLQTADANWKPCLLDEKA-KLFFKLVRLDNLFAYWNVNSELYSNHTPPEALRRLQQNVAVQNSIPHDYNFIFRPISANAKL--RMNPRSDVDFSSPKVDLVVNLPEVAVELSRPQYVSILELLGSVDMMSRNLPYRKYRPCVPVHNNAYNWWRYVISSVLEVNVKPRLHMWSWQHIRRHRQLAKQYKELYKTKITSKKPTEVVLKKLEDTEKTLDIFNITLARQQAEMEASKAGLRIYRPGVKMEEEQSQGWFGWMWNWSGDGSTPAKEAKPGGFDTL-------MTPAEKAKLYTAIGYSETAVNPNLPKSFEDMKVRFQLVSMSVSIKE-SREKPEIIRLSVIGLSATLTQRPSAQAIKIIAQLSTFEVTGLPRSNVAPMLLSSRRVVEENKPLLSLMFETNPLDAKADQRLHVESQPLEIIYDATTVNSLSAFFMPPNDLQLDELTNATRMKLEQFRDRTSTGLMYIIETQKVLDLNINLMASYVIVPQMGFYDPSHNLAILDLGHFQMSSQSKANLP-----QLLAG--SSTIEDIMSR-AYDSFDIQLSSLQFLYTKPDGDWKKARKERQSALHILEPVDLKVVFSRAMVVTDSRMPKFKMSGELPLLSLRISDDKVRGVLELIDSIPLPESRPPPRAAATQVTPKVLKQRDTPQL--TPRRPAMTD--LRS-SIIFDSEEDLFFDAPSSPIEDLPFFEASPGALERSASVKRRSMVKQDPKKNMTEFLMNFEISKLSIQLLR------LSAGQEVPVLHLEIEGLGTELKLRTFDMSSYTFLREICLECSEYQDGDGRK-VHLITTLDNTE-DDLLTLEYVKADKNAPDFKSAYKNTEQLIKVNFSSLDIHLHTEALLNSMNFLNS 1022          

HSP 2 Score: 513.842 bits (1322), Expect = 1.356e-145
Identity = 419/1210 (34.63%), Postives = 622/1210 (51.40%), Query Frame = 3
Query: 6672 CKSNTGVMYYYSVALSI--EEVQIVNSSETL-RTYKLDINVPLALHNQLPIKISYQ----SQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAK------EG-YSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQSLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSS-IVLYCNELVENAGWIKVPPGEA-VPFWPRSEVGKSMMMR---CKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINH-------------------------------LGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWN-QIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVE-GLEVDLSKK----LMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFV----SGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTG-KTALHSEV-----LNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKN---VIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEI 10094
            C+ ++   + + V +    + V  +N+ E   + Y L +   + L N LP  I+Y            +K G    + TA +   S       Y +++W     L    +E++ I F++       +G  S + +D+ VH+  + GQA V ++SP        Y +  K G   Q + D                           I +K    Y +  L                         + K + F +  K  L I+DS  SD FS+DTVGS G V CK +   Y VGVKI+ SS  LT++VT LP+YM++N++  ++  C E  +   W +  PGEA VPFWP  E  K M ++   C+     +   QP      + +L+ L+    GI V+V +TE + +I    Y +G A   LINH                               LG+D E         +EL  P  + V Y W +    R   W       +  N    D ++D   +E K + +SF     R++LFT +  + K    S  +E  +QEI+++L  +G+SLV+NS  QE+AYI ITSS V W  + ++K+R K L    +E LE  +  +V       + V+  ++ +E G +V L+       M++P    L R F P + ++Y  S  Q    ++I ++QI NQ+    FP VF P  PP+SV +D+  KP  ++S +   +G +  +R KYF+VLIQEM L +D GF+ +IL+      +G+V +  E  +F KD + ++  L   S   T        +++ FHISPIK+HLSFSLS     G KT   +E+     LNL L+S+G  +TD+QDV+FKL +FE         Q+   +  HY  QA++QMYVLVLGLDVLGNPFG+IRG+++GVE  FYEP +GA+ GPEEFVEG+A GVK+L G AVGG AGA SRI G M KG+AA+T+D +YQ+KRRE + K+P  L  GL +GGKGL+ GFV G+TG+VTKPI GA+K+G  GFFKGVGKGLVG VTRP  G++D ASS+F+GI+R A+T+ +V  +RPPRFI +DG+IR Y  RE  G+ +  KI             +  +  +K N    + +T + IF + +    G  + +WQ  +++    P I E +  +    KE+ + +F      K I
Sbjct: 2015 CRHHSSDAHVFKVNMVAVRDTVTSLNAQEEFDKPYVLHLWPTVLLRNLLPYPITYSLLAIGSPTPTQLKEGHSSQLHTAVMDHSSLELKLLGYLQQDWVAHYHLRNDHDEINFITFRSALQDDDVDGESSRAELDIAVHVKKEAGQAVVAVHSP--------YWMVNKTGRLLQYKADD--------------------------IHRKHPKDYDMPLL------------------------FSFKPRNFLQNNKVRLMISDSELSDDFSIDTVGSYGDVKCKGRAKDYLVGVKIDASSFSLTRLVTVLPFYMLVNRTKHVIRVCEEGQDE--WTEAQPGEAAVPFWPERE-SKKMKVKVDGCQTAAHTINFHQP-----ENCLLLHLDNTLGGIIVDVNLTEHSAVIRFSDYHDGAAPFLLINHTKDKTLEFYQSCKERRSSTWLPEDLLDLSLSLGEDRETRGSHQQEKEELA-PG-RAVYYTWAEPTGSRVLRWSWGPYSAELKNE--EDLLKDVN-DEGKLFVISFYEGLQRVVLFTTERRIYKLLCESERVELAEQEISLALQDMGISLVNNSTSQEIAYIGITSSDVVWEIKPKKKSRWKVLNGKDAEMLEQHYKSYVE-----SSPVDHSLLDLENGYQVCLTPNSVDMRMLQPYDAPLRRNFLPALKVEYSVSARQKAYRVQINRVQIQNQLPGAIFPFVFYPIKPPKSVRMDSEPKPFTDVSIITRAAGHSDILRIKYFKVLIQEMDLRMDLGFLYAILELFTAENAGTVTSEQEVELFEKDIEYLKTGLNPVSATDT----SPISLYEYFHISPIKLHLSFSLSSGGEDGNKTKRETEMIPVQSLNLLLKSIGATLTDVQDVVFKLAFFELTFQFRTTQQLQWEVIRHYSKQAIKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPYQGAIQGPEEFVEGMALGVKALVGGAVGGLAGAASRITGAMAKGVAAMTMDEEYQQKRREAMNKQPSGLKEGLTRGGKGLVSGFVSGITGIVTKPIKGAQKEGAAGFFKGVGKGLVGAVTRPTGGIIDMASSTFQGIKRAAETSQDVESLRPPRFIHEDGVIRPYKEREGFGSQMLQKIEN------GRFAKYRYFAHAKVNDSDFLMITKRGIFFVTKG-TFGQLTCEWQYLFDEFTKDPMIVEDRR-LRIEAKERVKSVFHAKEFGKII 3136          

HSP 3 Score: 248.054 bits (632), Expect = 8.273e-65
Identity = 236/926 (25.49%), Postives = 417/926 (45.03%), Query Frame = 3
Query: 3441 ITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTS-----VVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPES-KEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKN--DSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLA----SPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLT--AQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSF-------EIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIF---GAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGE 6146
            I A L  L+V +  EK   I +ISI G        +Y   VSI                        D+ +F  ++  +   T    Y +D+  VD  V + VG I++IFL  FV  +  F+N+FQE+ + +A    + +E AA  +  + E+  S+R  LD+++KAPV+++P++S S N ++ DLG +T KN F I  S S      +L+   + LS+L++ R+   +   + E  +L P+N+ + + RNLS +  H++P I I A +  +S+ +SQ D  +    L  N +E  + VP+   S K E  S K     +  GS  T    +  V+ G K                             K+K EN         N +M  SV+L+   +LD +    K I   +  ++   + +  + + + Q +   +   V +  +Y+C  +++L+ + D F+KA               +G+   P+  ++   A    +P +P  +  E+++ V  PEI+ V D+   D+ A+++ T   L       S  +   I+D + V CPF  + +  N   ++ P  +     +         L++  L + ++P +I ++  I + LT  A++ +E  +            +  + WE+R++ + +  F+ +            +G E    V  ++              G++L         LR+  I + LE+ V  + VPML+ + S  GD+KN  T I L   +++ V+Y+N+ + VWEPLLE  P E + +  F       +++    + SG+ DE D+  ++    + ISI   S+D L +T++K  L++LSNLG AF +A     +Q    F    AP+VVKN  G  V    ++ F  KP     + +     DGE
Sbjct: 1077 IRADLSCLKVFIRGEK-ARISEISIEG------KLHYIIAVSIA-----------------------DKEVFDFRMVNYVDATEGDAY-MDMQIVDTSVTLTVGCIQVIFLNKFVSSVLAFINNFQEAKEALAEVTVQAAEKAATGVLELAEK--STRISLDVDIKAPVVFLPQSSISTNVIVADLGLVTVKNHFEIVPSPSHTKIPPILDVMSVALSDLKMYRTTFKDEKFQTEIQLLKPVNMDLSIKRNLSASWYHSLPDIQINAHLKPMSLILSQEDMIVVLRTLSENLSETSDAVPAPKGSDKAETSSTK-----ESQGSGGTTVVTAAVVE-GQKA---------------------------AKLKIENE--------NDSMTASVKLAEC-LLDDKRKIVKMITSRMMAMRKGAEQNTMVDVNYRQGREGTTLETV-VQDLYLCASMEFLLTVADIFMKAM-------------AQGFSSPPKSNKQTSTATKDAAPAQPAASKMEMNIVVKNPEIVFVADLTRADAPALVMTTQCELLMKSDPNSQQMTAAITDLKVVACPFVHKQQDSNITTVLQPCQVFFTSKQSPTQPQAIELSINSLTLKVSPIIINTVITIQSALTPAAETPEELESP-----------VPPDLWERRDWKDLKLWFLEE------------EGEEDTQSVTPLI------------PQGESLQ--------LRISSICLTLEAGVGHRTVPMLLAKSSFHGDVKNWSTLINLHCHLNMEVNYYNEIMGVWEPLLE--PLEDDTKDGFRPWTLDLKMKKKQANRSGISDEVDY--IVPDYKTVISI--TSKDQLNITLTKCGLNMLSNLGTAFAEA----AKQTAECFQKDQAPFVVKNRLGLPVSVLYSEVF--KPIGVQSKGRTVELQDGE 1858          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Cavefish
Match: vps13c (vacuolar protein sorting 13 homolog C [Source:ZFIN;Acc:ZDB-GENE-070426-4])

HSP 1 Score: 518.85 bits (1335), Expect = 5.113e-147
Identity = 364/899 (40.49%), Postives = 537/899 (59.73%), Query Frame = 3
Query: 7368 KNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG-------WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSN--NLTSDGIEDFYINEM-KAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSK--KLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILD-FVSGSVPTYDEEAMFV--KDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA--LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHV-YCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTI 10010
            K   F+K  K  L ++ S WS+ FSLDTVGS G V C  K + Y VGV I +SS  LT+IVT  P+Y ++N+SS  L   E+  + G       W  +   E +P WP S  GK   +  +V   E      F   + +  L+ +E+ F GI V+V I++ + +I+   Y +G A   L+NH    V I F+Q        L   +   +AW D    R+  W  +G    HS   +L  D    F  + + + +WVSF+    R+LLFT D+ V   AR + +LE I QEI ISL ++GLSL++N  + E+AYI ITSSGV W ++R KNR K         LE ++    N L    A     I    GLEV+ ++    M  P    + R F  G+ ++ + SPHQ  +  +++ +Q+DNQ++   FP+VF P  PP+S+ALD+  KP I++S +   +  +  M++KYF VL+QEM + +DQGF+ +I   F S +    D++ M +  +D + ++A L+E+S    I        F++FHISPIK+HLS SL       +    +  + LNL L+S+G  +TD+ D+IFKL +FE +       +++ ++  HY  Q L+QMYVLVLGLDVLGNPFG+IRG+++GVE  FYEP +GAV GPEEF EG   GV+SL GH VGGAAG VSRI G++GKG+AA+T+D +YQ+KRRE++ + P + G  LA+GGKGL+ G V GVTG+VTKP++GAKK+G  GFFKG+GKGLVGVV RP  G+VD ASS+F+GI+RVA++  EV ++RP R I +DGIIR Y  +E+EG +++ +   +   +  E +  H  Y   +K  I +TN+R+  +   +++G ++ +W+  +E     P++
Sbjct: 2545 KKNIFSKN-KLQLCVSTSSWSEGFSLDTVGSYGCVRCPDKTMDYLVGVSIQMSSFNLTRIVTMSPFYTLVNKSSFELEVGEVQNDMGNGNVSGKWHYISSKECLPLWPESGTGK---LCVRVVGSESASKSFFFNKQDNGTLLSMEQ-FGGIIVDVNISDHSTVISFTDYYDGAAPALLVNHT-PWVTISFRQSGCEASRELNPGEAQRFAWDDPAGVRKLCWTCQG----HSGELDLIKDECGQFACDSLAQVHWVSFLDGRQRVLLFTEDVAVVTKARQAEELEQIQQEIIISLQNLGLSLINNHNRHEIAYIGITSSGVVW-EMRPKNRWKSFNRKNISLLEKSYQ---NHLSGKTAP--GWITLETGLEVNFARVPMQMRLPYSSPIRRNFLSGIHMELKQSPHQRSLRAQLHWLQVDNQLTGAIFPIVFHPVHPPKSIALDSEPKPFIDVSIITRFNEHSQVMQFKYFMVLVQEMAVKIDQGFLGAIFSLFTSPAQSQPDKQKMKLIDRDLEALQAQLMESS----ITDASGLSFFEHFHISPIKLHLSLSLGSSGDESEDGDMVAIQSLNLLLKSIGATLTDVDDLIFKLAFFEVKYQFYRREKLMWAVIGHYTEQFLKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPFQGAVQGPEEFAEGFVIGVRSLLGHTVGGAAGMVSRITGSVGKGLAAITMDKEYQQKRREEMNRPPRDFGESLAKGGKGLLKGVVGGVTGIVTKPVEGAKKEGAAGFFKGIGKGLVGVVARPTGGIVDMASSTFQGIQRVAESTEEVTKLRPVRLIREDGIIRPYDQQESEGYDLFQR--SEIKQLDGEYFREHYEYPGHRKTNIIVTNRRVMCVKEIDLIGHFNKEWECQFENFLKSPSV 3421          

HSP 2 Score: 460.299 bits (1183), Expect = 3.354e-129
Identity = 269/774 (34.75%), Postives = 438/774 (56.59%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEEN-KLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKA-----KKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILI--VSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVI 2321
            MV ESLV +L+N+++GDYV  LDKSQLK+G+ GGN  LENL +KENA   +    +     + KLTLKIP K+LY+E V+A LDGLY+L++P + +KYD  KE  Y  E KQKEL RIEE  ++     K + E KD+F EKLA Q++KNLQ+ +++IHIRYED  + P+R  ++G+TL+ LS QT D S  + I+ E + +  +K  +++ L  YWN N   F   +   +   +K  IS+ +  L    ++ +PI  SA M ++   E      P+++L L+++ I     R QY  +   L+S+D       +RK+RP+VP+  +   WW +  N +LE  +RR  RMWSW  I  HR   + Y   ++ KL   K+ +     I+  E+ LD+ NITL RQQA+M+      K + KK+  +   GG    +      +       +      + + +  T EEK+KLY AIGYSE + +  +P  YV++K+N +L +    + + +    +++K+ +  +S  + QR  A AI V   + +  VTG +   G+VP+L+++   S   +LL V FE NP + SA+Q + + ++P+EIIYD+ TVN +V+F    + + L  I++  L+ +EE+KE TA+GL H+ + R   D+++D+KPSY ++P+ G Y  +  ++++D GS+ +  V +     GS  + L          + + + AY++F + L ++Q L  + G     ++S  S +N I      E +  +C++
Sbjct:    1 MVLESLVSDLLNRFIGDYVENLDKSQLKIGIWGGNVVLENLRVKENALRVY----RCFLWFLGKLTLKIPLKNLYTEAVVATLDGLYLLVVPGATIKYDAVKEERYQQEAKQKELQRIEEALQIAARKEKAQEEKKDTFAEKLATQVIKNLQVKVTSIHIRYEDDVSDPSRPVSMGVTLSELSLQTTDESWRACILNE-AAKIIYKLGRLECLCAYWNVNSPIFYRGSWEEIVDKLKAGISTRDEDLKGYQYIFRPIFASAKMCINPNSEI-ELKSPKINLQLEVQNIAIEMTRPQYLSMVDLLESVDSMVKNAPYRKFRPDVPVHKHAKQWWLYGINSVLEVHIRRFNRMWSWSNIWKHRQTLKAYKAAYKSKLTQGKVREDTDKQIQELEKSLDVFNITLARQQAQMEVVRSGQKVVGKKVAAQKQGGGGGGFFSSFFGRKEGKKKEQEEEHRESESIDAIMTTEEKSKLYTAIGYSESSHNLALPKSYVAVKINFKLLRTSVTVREEA-NVPEILKIQMIDLSTTISQRPGAQAIKVEASLEHWYVTGLQ-QQGAVPSLIASVGASD-SSLLSVLFEINPEESSADQLLRVHSQPVEIIYDALTVNSLVEFFKAGKGVDLEVITSATLSKLEEIKEKTASGLSHIIETRKVLDLRIDLKPSYLLIPKSGFYHSNSDLMIIDFGSLHLSSVDQAAHQLGSSFSSL----------EEIMDRAYERFSLELRSVQVLYSKSGAHQITVQSLRSVENRIKTKVLPELSTDRCLV 755          

HSP 3 Score: 198.749 bits (504), Expect = 8.553e-50
Identity = 226/1064 (21.24%), Postives = 453/1064 (42.58%), Query Frame = 3
Query: 2835 EIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSD-FISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXX----------XMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDF--EDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAF 5975
            ++G    + ++DM    YI ++ +   +F D +   P+C+  +     G  LL +  +   K  P +VT+  +    L  QF S+++ LH EALL  ++F+++ +   +    E+          ETR+                                 + + RT   ++  VS  SD +              +T   I   L    V+V +++  NI DI I G+   +      T +   + D  I +  P  ++KK I I  DE +FS  ++L  + T   GY  D S+VD  V + VG I++++L+ FV+ +  F N FQ + + ++    + +  A + + +  ++  S R  L+I +KAP+I IP++S S NA+  DLG +T  N F++    +     V++   + L+ L++SR    +++++        I I +++ + +                    +V +SQ D  +   +L  N  EA +  P  P S +    +  +P               I  + G   E +                      CL + +   +   G M  N  +EVS  LS   + D +    +  + ++     DS+D  I + + Q     S + V +  +Y+C  V++LM + +FF++A P    P             +++P +   +  +K       +P +  +    + + V  PE++ V +++  D+ A++       ++   +E+ ++  ++ DF+ + CPF    R K+   ++ P S+ L      ++ + GS+T+E +II ++P ++ ++  I   +T ++ +E  T             + + W  R      + F+               GVE+  +V +   +V+  +  + F          E K+      ++V LES    + +P+L+ E S+    +N  + + L   +++ V+YFN+   VWEPL+E   E   R+++  ++     ++ + ++     +D + +      I + S+D + +T+SK  L+VL NL +  
Sbjct: 1288 QLGVHTKVHKYDMQATTYIKTISMKCLEFTD-ACGDPLCILSSSAEA-GAELLKVEYV---KVCPPYVTLSPSNNQSL-VQFSSLDLMLHTEALLSTMDFLSAALSSSSSPSPER----------ETRR---------------------------------SDDSRTSSARSTVVSSPSDGD--------------VTDLKIDMQLGAFNVLVCDQR-CNIADIRIQGLHGSLLMQGTQTCILARLRDFIIINVDPKTIHKKAISIVGDE-VFSFSMSLTPKATEGVGYT-DTSKVDGRVKLNVGCIQVVYLHKFVMSLLNFSNHFQTAKEALSAATAQAAVKAVSSVRDFAQK--SFRLSLNIRLKAPLIIIPQSSTSNNAVEADLGLITVSNSFSLLPVDNCPLPAVIDNMDVQLTQLKLSR---YQSSLE--------IPILLELKKKMD----------------CYCNVALSQDDLTVLLKILMENIGEAGSVQP--PSSSDSAYRQTAVP---------------IRTRLGVHSESV----------------------CLGEFRLCEMRAAGKMFSNGNLEVSTVLSACTLDDRRSGIQRVTSRMLGKRDEDSADAMIDVTYSQSGGERSVIAV-LQKLYLCGSVEFLMAVGEFFLQALPKTQPPAPALPPAPAPDKPSLLPFKHVSE--QKINSGAKTAPGQKMV----LRMVVVDPEVVFVANLMKADAPALVASFQCDFRFDSQEETQLMKANVKDFKVLACPF---IRTKDNQPVLRPCSVVLETRMSDSSPLTGSVTVEEVIIKVSPIILNTVLTITAAMTPRAPEERGTAAAP-------ETLSSLWSVRNIYTCNYWFL---------------GVETAAEVTESFCEVDGGKEGESF--------RAEVKV------VQVTLESGQGHRTIPLLLAESSITACARNWSSTLSLAADITLEVNYFNEAHAVWEPLIERV-ESGKRRWNLTVQM---KNNPVREKSPVPGDDFVMLPDPRTGINICSKDTMNITLSKCCLNVLHNLAQVL 2167          

HSP 4 Score: 127.487 bits (319), Expect = 2.950e-28
Identity = 87/299 (29.10%), Postives = 165/299 (55.18%), Query Frame = 3
Query: 3426 MTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTS----VVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEA 4310
            +  + + A L    VIV +++ ++I +I I G+ A V      TEV   + D+ + D  P  ++KK++ I  +E +FS +++L+   T   GY  D +RVD  V + +G I+I +L+ F+  + TF+++FQ + + ++    + +E AA+ + +  ++  S R  L+I +KAP+I IP++S S NA +VDLG +T  N F++  +       V+E   I L++L++ R        ++E  +L PIN+ + + RNL+ +   +IP + +   + S+++ + Q D  +   +L  N  E 
Sbjct:  951 VVSFKLYAMLGSFGVIVCDDR-SSIANIQIKGVDASVVVQTKETEVFARLRDIVVMDVDPKTIHKKVVSIVGEE-VFSFKMSLYPGATEGDGY-CDTARVDSRVTLRLGCIQITYLHKFLTSLMTFVDNFQTAKEALSAATAQAAEKAASSVRDFAQK--SFRLSLNIRLKAPLIIIPQSSTSHNAFVVDLGLITVSNSFSLLPAEGFPLPAVMETMDINLTHLKLCR--YCHQRPQSEVELLQPINLELLVKRNLAASWFTSIPGVEVTGVLKSMNISLGQEDLVVLMSILAENIGEG 1242          

HSP 5 Score: 55.4546 bits (132), Expect = 1.610e-6
Identity = 34/143 (23.78%), Postives = 70/143 (48.95%), Query Frame = 3
Query: 6762 TYKLDINVPLALHNQLPIKISY--QSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAV 7184
             Y + ++ P+ + N LP  I Y  +      +++ G    V  A +  +    V   YQ + W   + + P + E   + F + +   +  +D+ +H++   G+  ++++SPYW++NKT   L Y+A +D  V+H +   D +
Sbjct: 2399 AYVIHLHPPVTVRNLLPYSIRYLLEGSADFQELQEGGTADVLNASISGEIMELVLVKYQGRNWNSHVKISPDVPEFFPVCFTS-DSSEQLTVDVCLHVSRAAGRMVLSVFSPYWIINKTSRVLQYRA-EDTYVKHPSDFRDVI 2539          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Cavefish
Match: vps13c (vacuolar protein sorting 13 homolog C [Source:ZFIN;Acc:ZDB-GENE-070426-4])

HSP 1 Score: 518.85 bits (1335), Expect = 5.460e-147
Identity = 363/899 (40.38%), Postives = 539/899 (59.96%), Query Frame = 3
Query: 7368 KNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG-------WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSN--NLTSDGIEDFYINEM-KAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSK--KLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILD-FVSGSVPTYDEEAMFV--KDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA--LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHV-YCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTI 10010
            K   F+K  K  L ++ S WS+ FSLDTVGS G V C  K + Y VGV I +SS  LT+IVT  P+Y ++N+SS  L   E+  + G       W  +   E +P WP S  GK   +  +V   E      F   + +  L+ +E+ F GI V+V I++ + +I+   Y +G A   L+NH    V I F+Q        L   +   +AW D    R+  W  +G    HS   +L  D    F  + + + +WVSF+    R+LLFT D+ V   AR + +LE I QEI ISL ++GLSL++N  + E+AYI ITSSGV W ++R KNR K         LE ++   ++  KT+       I    GLEV+ ++    M  P    + R F  G+ ++ + SPHQ  +  +++ +Q+DNQ++   FP+VF P  PP+S+ALD+  KP I++S +   +  +  M++KYF VL+QEM + +DQGF+ +I   F S +    D++ M +  +D + ++A L+E+S    I        F++FHISPIK+HLS SL       +    +  + LNL L+S+G  +TD+ D+IFKL +FE +       +++ ++  HY  Q L+QMYVLVLGLDVLGNPFG+IRG+++GVE  FYEP +GAV GPEEF EG   GV+SL GH VGGAAG VSRI G++GKG+AA+T+D +YQ+KRRE++ + P + G  LA+GGKGL+ G V GVTG+VTKP++GAKK+G  GFFKG+GKGLVGVV RP  G+VD ASS+F+GI+RVA++  EV ++RP R I +DGIIR Y  +E+EG +++ +   +   +  E +  H  Y   +K  I +TN+R+  +   +++G ++ +W+  +E     P++
Sbjct: 2429 KKNIFSKN-KLQLCVSTSSWSEGFSLDTVGSYGCVRCPDKTMDYLVGVSIQMSSFNLTRIVTMSPFYTLVNKSSFELEVGEVQNDMGNGNVSGKWHYISSKECLPLWPESGTGK---LCVRVVGSESASKSFFFNKQDNGTLLSMEQ-FGGIIVDVNISDHSTVISFTDYYDGAAPALLVNHT-PWVTISFRQSGCEASRELNPGEAQRFAWDDPAGVRKLCWTCQG----HSGELDLIKDECGQFACDSLAQVHWVSFLDGRQRVLLFTEDVAVVTKARQAEELEQIQQEIIISLQNLGLSLINNHNRHEIAYIGITSSGVVW-EMRPKNRWKSFNRKNISLLEKSYQNHLSG-KTAPG----WITLETGLEVNFARVPMQMRLPYSSPIRRNFLSGIHMELKQSPHQRSLRAQLHWLQVDNQLTGAIFPIVFHPVHPPKSIALDSEPKPFIDVSIITRFNEHSQVMQFKYFMVLVQEMAVKIDQGFLGAIFSLFTSPAQSQPDKQKMKLIDRDLEALQAQLMESS----ITDASGLSFFEHFHISPIKLHLSLSLGSSGDESEDGDMVAIQSLNLLLKSIGATLTDVDDLIFKLAFFEVKYQFYRREKLMWAVIGHYTEQFLKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPFQGAVQGPEEFAEGFVIGVRSLLGHTVGGAAGMVSRITGSVGKGLAAITMDKEYQQKRREEMNRPPRDFGESLAKGGKGLLKGVVGGVTGIVTKPVEGAKKEGAAGFFKGIGKGLVGVVARPTGGIVDMASSTFQGIQRVAESTEEVTKLRPVRLIREDGIIRPYDQQESEGYDLFQR--SEIKQLDGEYFREHYEYPGHRKTNIIVTNRRVMCVKEIDLIGHFNKEWECQFENFLKSPSV 3305          

HSP 2 Score: 439.113 bits (1128), Expect = 1.015e-122
Identity = 262/759 (34.52%), Postives = 427/759 (56.26%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEEN-KLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKA-----KKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILI--VSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHI 2276
            MV ESLV +L+N+++GDYV  LDKSQLK+G+ GG +      I+  +  +  L    R G   KLTLKIP K+LY+E V+A LDGLY+L++P + +KYD  KE  Y  E KQKEL RIEE  ++     K + E KD+F EKLA Q++KNLQ+ +++IHIRYED  + P+R  ++G+TL+ LS QT D S  + I+ E + +  +K  +++ L  YWN N   F   +   +   +K  IS+ +  L    ++ +PI  SA M ++   E      P+++L L+++ I     R QY  +   L+S+D       +RK+RP+VP+  +   WW +  N +LE  +RR  RMWSW  I  HR   + Y   ++ KL   K+ +     I+  E+ LD+ NITL RQQA+M+      K + KK+  +   GG    +      +       +      + + +  T EEK+KLY AIGYSE + +  +P  YV++K+N +L +    + + +    +++K+ +  +S  + QR  A AI V   + +  VTG +   G+VP+L+++   S   +LL V FE NP + SA+Q + + ++P+EIIYD+ TVN +V+F    + + L  I++  L+ +EE+KE TA+GL H+ + R   D+++D+KPSY ++P+ G Y  +  ++++D GS+ +  V +     GS  + L          + + + AY++F + L ++Q L  + G     ++S  S +N I
Sbjct:    1 MVLESLVSDLLNRFIGDYVENLDKSQLKIGIWGGRSRALLHFIRPESGSETGL----RSGQW-KLTLKIPLKNLYTEAVVATLDGLYLLVVPGATIKYDAVKEERYQQEAKQKELQRIEEALQIAARKEKAQEEKKDTFAEKLATQVIKNLQVKVTSIHIRYEDDVSDPSRPVSMGVTLSELSLQTTDESWRACILNE-AAKIIYKLGRLECLCAYWNVNSPIFYRGSWEEIVDKLKAGISTRDEDLKGYQYIFRPIFASAKMCINPNSEI-ELKSPKINLQLEVQNIAIEMTRPQYLSMVDLLESVDSMVKNAPYRKFRPDVPVHKHAKQWWLYGINSVLEVHIRRFNRMWSWSNIWKHRQTLKAYKAAYKSKLTQGKVREDTDKQIQELEKSLDVFNITLARQQAQMEVVRSGQKVVGKKVAAQKQGGGGGGFFSSFFGRKEGKKKEQEEEHRESESIDAIMTTEEKSKLYTAIGYSESSHNLALPKSYVAVKINFKLLRTSVTVREEA-NVPEILKIQMIDLSTTISQRPGAQAIKVEASLEHWYVTGLQ-QQGAVPSLIASVGASD-SSLLSVLFEINPEESSADQLLRVHSQPVEIIYDALTVNSLVEFFKAGKGVDLEVITSATLSKLEEIKEKTASGLSHIIETRKVLDLRIDLKPSYLLIPKSGFYHSNSDLMIIDFGSLHLSSVDQAAHQLGSSFSSL----------EEIMDRAYERFSLELRSVQVLYSKSGAHQITVQSLRSVENRI 739          

HSP 3 Score: 182.956 bits (463), Expect = 4.407e-45
Identity = 192/866 (22.17%), Postives = 392/866 (45.27%), Query Frame = 3
Query: 3426 MTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPP-------DKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSD-FISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKN--PRKY-RKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPF-RSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAF 5975
            +T   I   L    V+V +++  NI DI I G+   +      T +   + D  I +  P  ++KK I I  DE +FS  ++L  + T   GY  D S+VD  V + VG I++++L+ FV+ +  F N FQ + + ++    + +  A + + +  ++  S R  L+I +KAP+I IP++S S NA+  DLG +T  N F++    +     V++   + L+ L++SR    +++++        I I +++ + +                    +V +SQ D  +   +L  N  EA +  P  P S +    +  +P   +    R     + T      +++L ++  + D     + S++ P         CL + +   +   G M  N  +EVS  LS   + D +    +  + ++     DS+D  I + + Q     S + V +  +Y+C  V++LM + +FF++A P    P           + +  P K+  ++ + +P +  +    + + V  PE++ V +++  D+ A++       ++   +E+ ++  ++ DF+ + CPF R++  +     ++ P S+ L      ++ + GS+T+E +II ++P ++ ++  I   +T ++ +E  T             + + W  R      + F+               GVE+  +V +   +V+  +  + F          E K+      ++V LES    + +P+L+ E S+    +N  + + L   +++ V+YFN+   VWEPL+E   E   R+++  ++ +S +          +D + +      I + S+D + +T+SK  L+VL NL +  
Sbjct: 1290 VTDLKIDMQLGAFNVLVCDQR-CNIADIRIQGLHGSLLMQGTQTCILARLRDFIIINVDPKTIHKKAISIVGDE-VFSFSMSLTPKATEGVGYT-DTSKVDGRVKLNVGCIQVVYLHKFVMSLLNFSNHFQTAKEALSAATAQAAVKAVSSVRDFAQK--SFRLSLNIRLKAPLIIIPQSSTSNNAVEADLGLITVSNSFSLLPVDNCPLPAVIDNMDVQLTQLKLSR---YQSSLE--------IPILLELKKKMD----------------CYCNVALSQDDLTVLLKILMENIGEAGSVQP--PSSSDSAYRQTAVPIRTR----RCIKQINAT-----SLQYLTLKTLQTD-----FTSKISPCLGVHSESVCLGEFRLCEMRAAGKMFSNGNLEVSTVLSACTLDDRRSGIQRVTSRMLGKRDEDSADAMIDVTYSQSGGERSVIAV-LQKLYLCGSVEFLMAVGEFFLQALPKTQPPAPALPPAPAPDKPSLLPFKHVSEQKINTPGQKMV----LRMVVVDPEVVFVANLMKADAPALVASFQCDFRFDSQEETQLMKANVKDFKVLACPFIRTKDNQPAPPYVLRPCSVVLETRMSDSSPLTGSVTVEEVIIKVSPIILNTVLTITAAMTPRAPEERGTAAAP-------ETLSSLWSVRNIYTCNYWFL---------------GVETAAEVTESFCEVDGGKEGESF--------RAEVKV------VQVTLESGQGHRTIPLLLAESSITACARNWSSTLSLAADITLEVNYFNEAHAVWEPLIERV-ESGKRRWNLTVQ-VSNNPVREKSPVPGDDFVMLPDPRTGINICSKDTMNITLSKCCLNVLHNLAQVL 2064          

HSP 4 Score: 126.331 bits (316), Expect = 5.399e-28
Identity = 100/362 (27.62%), Postives = 192/362 (53.04%), Query Frame = 3
Query: 3426 MTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTS----VVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQV------PSVPESKEEVKSR-KILPKDDKSGSA----RTASDESITVQFGFKMEFLDVEL 4466
            +  + + A L    VIV +++ ++I +I I G+ A V      TEV   + D+ + D  P  ++KK++ I  +E +FS +++L+   T   GY  D +RVD  V + +G I+I +L+ F+  + TF+++FQ + + ++    + +E AA+ + +  ++  S R  L+I +KAP+I IP++S S NA +VDLG +T  N F++  +       V+E   I L++L++ R        ++E  +L PIN+ + + RNL+ +   +IP + +   + S++V +   D  +   +L  N  E    +      P++ +  E+ +   ++LP +  S S     R+ S+  ++V   F  E ++V L
Sbjct:  848 VVSFKLYAMLGSFGVIVCDDR-SSIANIQIKGVDASVVVQTKETEVFARLRDIVVMDVDPKTIHKKVVSIVGEE-VFSFKMSLYPGATEGDGY-CDTARVDSRVTLRLGCIQITYLHKFLTSLMTFVDNFQTAKEALSAATAQAAEKAASSVRDFAQK--SFRLSLNIRLKAPLIIIPQSSTSHNAFVVDLGLITVSNSFSLLPAEGFPLPAVMETMDINLTHLKLCR--YCHQRPQSEVELLQPINLELLVKRNLAASWFTSIPGVEVTGVLKSMNVSLLCEDLVVLMSILAENIGEGSRTMSPDLKKPAIEDKSEQSEEHSEVLPLETGSQSTITNERSFSENIVSVLLNF--ELMEVTL 1200          

HSP 5 Score: 55.4546 bits (132), Expect = 1.501e-6
Identity = 34/143 (23.78%), Postives = 70/143 (48.95%), Query Frame = 3
Query: 6762 TYKLDINVPLALHNQLPIKISY--QSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAV 7184
             Y + ++ P+ + N LP  I Y  +      +++ G    V  A +  +    V   YQ + W   + + P + E   + F + +   +  +D+ +H++   G+  ++++SPYW++NKT   L Y+A +D  V+H +   D +
Sbjct: 2283 AYVIHLHPPVTVRNLLPYSIRYLLEGSADFQELQEGGTADVLNASISGEIMELVLVKYQGRNWNSHVKISPDVPEFFPVCFTS-DSSEQLTVDVCLHVSRAAGRMVLSVFSPYWIINKTSRVLQYRA-EDTYVKHPSDFRDVI 2423          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Cavefish
Match: vps13c (vacuolar protein sorting 13 homolog C [Source:ZFIN;Acc:ZDB-GENE-070426-4])

HSP 1 Score: 504.212 bits (1297), Expect = 1.398e-142
Identity = 352/834 (42.21%), Postives = 506/834 (60.67%), Query Frame = 3
Query: 7368 KNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG-------WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSN--NLTSDGIEDFYINEM-KAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSK--KLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILD-FVSGSVPTYDEEAMFV--KDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA--LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIW 9818
            K   F+K  K  L ++ S WS+ FSLDTVGS G V C  K + Y VGV I +SS  LT+IVT  P+Y ++N+SS  L   E+  + G       W  +   E +P WP S  GK   +  +V   E      F   + +  L+ +E+ F GI V+V I++ + +I+   Y +G A   L+NH    V I F+Q        L   +   +AW D    R+  W  +G    HS   +L  D    F  + + + +WVSF+    R+LLFT D+ V   AR + +LE I QEI ISL ++GLSL++N  + E+AYI ITSSGV W ++R KNR K         LE ++    N L    A     I    GLEV+ ++    M  P    + R F  G+ ++ + SPHQ  +  +++ +Q+DNQ++   FP+VF P  PP+S+ALD+  KP I++S +   +  +  M++KYF VL+QEM + +DQGF+ +I   F S +    D++ M +  +D + ++A L+E+S    I        F++FHISPIK+HLS SL       +    +  + LNL L+S+G  +TD+ D+IFKL +FE +       +++ ++  HY  Q L+QMYVLVLGLDVLGNPFG+IRG+++GVE  FYEP +GAV GPEEF EG   GV+SL GH VGGAAG VSRI G++GKG+AA+T+D +YQ+KRRE++ + P + G  LA+GGKGL+ G V GVTG+VTKP++GAKK+G  GFFKG+GKGLVGVV RP  G+VD ASS+F+GI+RVA++  EV ++RP R I +DGIIR Y  +E+EG +++
Sbjct: 2578 KKNIFSKN-KLQLCVSTSSWSEGFSLDTVGSYGCVRCPDKTMDYLVGVSIQMSSFNLTRIVTMSPFYTLVNKSSFELEVGEVQNDMGNGNVSGKWHYISSKECLPLWPESGTGK---LCVRVVGSESASKSFFFNKQDNGTLLSMEQ-FGGIIVDVNISDHSTVISFTDYYDGAAPALLVNHT-PWVTISFRQSGCEASRELNPGEAQRFAWDDPAGVRKLCWTCQG----HSGELDLIKDECGQFACDSLAQVHWVSFLDGRQRVLLFTEDVAVVTKARQAEELEQIQQEIIISLQNLGLSLINNHNRHEIAYIGITSSGVVW-EMRPKNRWKSFNRKNISLLEKSYQ---NHLSGKTAP--GWITLETGLEVNFARVPMQMRLPYSSPIRRNFLSGIHMELKQSPHQRSLRAQLHWLQVDNQLTGAIFPIVFHPVHPPKSIALDSEPKPFIDVSIITRFNEHSQVMQFKYFMVLVQEMAVKIDQGFLGAIFSLFTSPAQSQPDKQKMKLIDRDLEALQAQLMESS----ITDASGLSFFEHFHISPIKLHLSLSLGSSGDESEDGDMVAIQSLNLLLKSIGATLTDVDDLIFKLAFFEVKYQFYRREKLMWAVIGHYTEQFLKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPFQGAVQGPEEFAEGFVIGVRSLLGHTVGGAAGMVSRITGSVGKGLAAITMDKEYQQKRREEMNRPPRDFGESLAKGGKGLLKGVVGGVTGIVTKPVEGAKKEGAAGFFKGIGKGLVGVVARPTGGIVDMASSTFQGIQRVAESTEEVTKLRPVRLIREDGIIRPYDQQESEGYDLF 3391          

HSP 2 Score: 446.047 bits (1146), Expect = 8.042e-125
Identity = 272/807 (33.71%), Postives = 441/807 (54.65%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIP----------NSEMKYDPEKERSYLFETKQKELNRIEE---------------------NKLKRNDPKKET---ETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKA-----KKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILI--VSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVI 2321
            MV ESLV +L+N+++GDYV  LDKSQLK+G+ GGN  LENL +KENA   +    +     + KLTLKIP K+LY+E V+A LDGLY+L++P          N  +KYD  KE  Y  E KQKEL RIEE                      + K+++ K E    E KD+F EKLA Q++KNLQ+ +++IHIRYED  + P+R  ++G+TL+ LS QT D S  + I+ E + +  +K  +++ L  YWN N   F   +   +   +K  IS+ +  L    ++ +PI  SA M ++   E      P+++L L+++ I     R QY  +   L+S+D       +RK+RP+VP+  +   WW +  N +LE  +RR  RMWSW  I  HR   + Y   ++ KL   K+ +     I+  E+ LD+ NITL RQQA+M+      K + KK+  +   GG    +      +       +      + + +  T EEK+KLY AIGYSE + +  +P  YV++K+N +L +    + + +    +++K+ +  +S  + QR  A AI V   + +  VTG +   G+VP+L+++   S   +LL V FE NP + SA+Q + + ++P+EIIYD+ TVN +V+F    + + L  I++  L+ +EE+KE TA+GL H+ + R   D+++D+KPSY ++P+ G Y  +  ++++D GS+ +  V +     GS  + L          + + + AY++F + L ++Q L  + G     ++S  S +N I      E +  +C++
Sbjct:    1 MVLESLVSDLLNRFIGDYVENLDKSQLKIGIWGGNVVLENLRVKENALRVY----RCFLWFLGKLTLKIPLKNLYTEAVVATLDGLYLLVVPGASEYCFSVFNLSIKYDAVKEERYQQEAKQKELQRIEEALQIAARKDKGRKHKKHKKTFRRCKKHEQKSEKAQEEKKDTFAEKLATQVIKNLQVKVTSIHIRYEDDVSDPSRPVSMGVTLSELSLQTTDESWRACILNE-AAKIIYKLGRLECLCAYWNVNSPIFYRGSWEEIVDKLKAGISTRDEDLKGYQYIFRPIFASAKMCINPNSEI-ELKSPKINLQLEVQNIAIEMTRPQYLSMVDLLESVDSMVKNAPYRKFRPDVPVHKHAKQWWLYGINSVLEVHIRRFNRMWSWSNIWKHRQTLKAYKAAYKSKLTQGKVREDTDKQIQELEKSLDVFNITLARQQAQMEVVRSGQKVVGKKVAAQKQGGGGGGFFSSFFGRKEGKKKEQEEEHRESESIDAIMTTEEKSKLYTAIGYSESSHNLALPKSYVAVKINFKLLRTSVTVREEA-NVPEILKIQMIDLSTTISQRPGAQAIKVEASLEHWYVTGLQ-QQGAVPSLIASVGASD-SSLLSVLFEINPEESSADQLLRVHSQPVEIIYDALTVNSLVEFFKAGKGVDLEVITSATLSKLEEIKEKTASGLSHIIETRKVLDLRIDLKPSYLLIPKSGFYHSNSDLMIIDFGSLHLSSVDQAAHQLGSSFSSL----------EEIMDRAYERFSLELRSVQVLYSKSGAHQITVQSLRSVENRIKTKVLPELSTDRCLV 788          

HSP 3 Score: 198.364 bits (503), Expect = 9.685e-50
Identity = 226/1064 (21.24%), Postives = 453/1064 (42.58%), Query Frame = 3
Query: 2835 EIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI----DQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSD-FISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXX----------XMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDF--EDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAF 5975
            ++G    + ++DM    YI ++ +   +F D +   P+C+  +     G  LL +  +   K  P +VT+  +    L  QF S+++ LH EALL  ++F+++ +   +    E+          ETR+                                 + + RT   ++  VS  SD +              +T   I   L    V+V +++  NI DI I G+   +      T +   + D  I +  P  ++KK I I  DE +FS  ++L  + T   GY  D S+VD  V + VG I++++L+ FV+ +  F N FQ + + ++    + +  A + + +  ++  S R  L+I +KAP+I IP++S S NA+  DLG +T  N F++    +     V++   + L+ L++SR    +++++        I I +++ + +                    +V +SQ D  +   +L  N  EA +  P  P S +    +  +P               I  + G   E +                      CL + +   +   G M  N  +EVS  LS   + D +    +  + ++     DS+D  I + + Q     S + V +  +Y+C  V++LM + +FF++A P    P             +++P +   +  +K       +P +  +    + + V  PE++ V +++  D+ A++       ++   +E+ ++  ++ DF+ + CPF    R K+   ++ P S+ L      ++ + GS+T+E +II ++P ++ ++  I   +T ++ +E  T             + + W  R      + F+               GVE+  +V +   +V+  +  + F          E K+      ++V LES    + +P+L+ E S+    +N  + + L   +++ V+YFN+   VWEPL+E   E   R+++  ++     ++ + ++     +D + +      I + S+D + +T+SK  L+VL NL +  
Sbjct: 1321 QLGVHTKVHKYDMQATTYIKTISMKCLEFTD-ACGDPLCILSSSAEA-GAELLKVEYV---KVCPPYVTLSPSNNQSL-VQFSSLDLMLHTEALLSTMDFLSAALSSSSSPSPER----------ETRR---------------------------------SDDSRTSSARSTVVSSPSDGD--------------VTDLKIDMQLGAFNVLVCDQR-CNIADIRIQGLHGSLLMQGTQTCILARLRDFIIINVDPKTIHKKAISIVGDE-VFSFSMSLTPKATEGVGYT-DTSKVDGRVKLNVGCIQVVYLHKFVMSLLNFSNHFQTAKEALSAATAQAAVKAVSSVRDFAQK--SFRLSLNIRLKAPLIIIPQSSTSNNAVEADLGLITVSNSFSLLPVDNCPLPAVIDNMDVQLTQLKLSR---YQSSLE--------IPILLELKKKMD----------------CYCNVALSQDDLTVLLKILMENIGEAGSVQP--PSSSDSAYRQTAVP---------------IRTRLGVHSESV----------------------CLGEFRLCEMRAAGKMFSNGNLEVSTVLSACTLDDRRSGIQRVTSRMLGKRDEDSADAMIDVTYSQSGGERSVIAV-LQKLYLCGSVEFLMAVGEFFLQALPKTQPPAPALPPAPAPDKPSLLPFKHVSE--QKINSGAKTAPGQKMV----LRMVVVDPEVVFVANLMKADAPALVASFQCDFRFDSQEETQLMKANVKDFKVLACPF---IRTKDNQPVLRPCSVVLETRMSDSSPLTGSVTVEEVIIKVSPIILNTVLTITAAMTPRAPEERGTAAAP-------ETLSSLWSVRNIYTCNYWFL---------------GVETAAEVTESFCEVDGGKEGESF--------RAEVKV------VQVTLESGQGHRTIPLLLAESSITACARNWSSTLSLAADITLEVNYFNEAHAVWEPLIERV-ESGKRRWNLTVQM---KNNPVREKSPVPGDDFVMLPDPRTGINICSKDTMNITLSKCCLNVLHNLAQVL 2200          

HSP 4 Score: 127.487 bits (319), Expect = 2.766e-28
Identity = 87/299 (29.10%), Postives = 165/299 (55.18%), Query Frame = 3
Query: 3426 MTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTS----VVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEA 4310
            +  + + A L    VIV +++ ++I +I I G+ A V      TEV   + D+ + D  P  ++KK++ I  +E +FS +++L+   T   GY  D +RVD  V + +G I+I +L+ F+  + TF+++FQ + + ++    + +E AA+ + +  ++  S R  L+I +KAP+I IP++S S NA +VDLG +T  N F++  +       V+E   I L++L++ R        ++E  +L PIN+ + + RNL+ +   +IP + +   + S+++ + Q D  +   +L  N  E 
Sbjct:  984 VVSFKLYAMLGSFGVIVCDDR-SSIANIQIKGVDASVVVQTKETEVFARLRDIVVMDVDPKTIHKKVVSIVGEE-VFSFKMSLYPGATEGDGY-CDTARVDSRVTLRLGCIQITYLHKFLTSLMTFVDNFQTAKEALSAATAQAAEKAASSVRDFAQK--SFRLSLNIRLKAPLIIIPQSSTSHNAFVVDLGLITVSNSFSLLPAEGFPLPAVMETMDINLTHLKLCR--YCHQRPQSEVELLQPINLELLVKRNLAASWFTSIPGVEVTGVLKSMNISLGQEDLVVLMSILAENIGEG 1275          

HSP 5 Score: 55.4546 bits (132), Expect = 1.593e-6
Identity = 34/143 (23.78%), Postives = 70/143 (48.95%), Query Frame = 3
Query: 6762 TYKLDINVPLALHNQLPIKISY--QSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAV 7184
             Y + ++ P+ + N LP  I Y  +      +++ G    V  A +  +    V   YQ + W   + + P + E   + F + +   +  +D+ +H++   G+  ++++SPYW++NKT   L Y+A +D  V+H +   D +
Sbjct: 2432 AYVIHLHPPVTVRNLLPYSIRYLLEGSADFQELQEGGTADVLNASISGEIMELVLVKYQGRNWNSHVKISPDVPEFFPVCFTS-DSSEQLTVDVCLHVSRAAGRMVLSVFSPYWIINKTSRVLQYRA-EDTYVKHPSDFRDVI 2572          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003418.1 (pep scaffold:Pmarinus_7.0:GL489749:646:4444:-1 gene:ENSPMAG00000003128.1 transcript:ENSPMAT00000003418.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 113.62 bits (283), Expect = 1.257e-29
Identity = 53/91 (58.24%), Postives = 69/91 (75.82%), Query Frame = 3
Query:  123 GNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLK 395
            G+A LENL +KENA  + D+P KV+ GHI KLTLKIPWK+LY E VIA L+G+Y+L++P++ +KYD EKE     E KQ+EL RIEE  +K
Sbjct:    1 GDAVLENLVLKENALSELDVPFKVKVGHIGKLTLKIPWKNLYKEAVIASLEGVYLLVVPSASIKYDAEKEEKQQLELKQRELRRIEEAMMK 91          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003332.1 (pep scaffold:Pmarinus_7.0:GL487813:5059:8070:-1 gene:ENSPMAG00000003052.1 transcript:ENSPMAT00000003332.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 111.309 bits (277), Expect = 4.859e-28
Identity = 83/142 (58.45%), Postives = 109/142 (76.76%), Query Frame = 3
Query: 9393 SRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRR-VADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNI 9815
            SRI GTMGKG+A +T+D +YQRKRRE++ ++P +L  GLA+GGKGL+ G + GVTG++TKPI+GA+  G  GF KGVGKGLVGVV RP  GV+D ASS+ EGI+R  A+ +  V ++RPPR I +DGIIR Y  RE EG+ +
Sbjct:    8 SRITGTMGKGLATITMDEEYQRKRREEMNQQPTDLKEGLARGGKGLLKGVIGGVTGIITKPIEGARSGGATGFLKGVGKGLVGVVARPAGGVIDMASSTMEGIKRWQAEASETVEKMRPPRVIQEDGIIRPYSRREGEGSQL 149          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Yeast
Match: VPS13 (Protein involved in prospore membrane morphogenesis; peripheral membrane protein that localizes to the prospore membrane and at numerous membrane contact sites; involved in sporulation, vacuolar protein sorting, prospore membrane formation during sporulation, and protein-Golgi retention; required for mitochondrial integrity; contains a PH-like domain; homologous to human CHAC and COH1 which are involved in Chorea-acanthocytosis and Cohen syndrome, respectively [Source:SGD;Acc:S000003963])

HSP 1 Score: 187.578 bits (475), Expect = 2.334e-47
Identity = 196/847 (23.14%), Postives = 365/847 (43.09%), Query Frame = 3
Query:   69 GDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKR---------NDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDT----STCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREF-FKSVKMDSLAVYWNSNPKPFSS---ENRATLKAI--MKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCE----------EELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEF--TQEEKAKLYQAIGYSELTTDP---DIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLV--------STQAQSKLMN------------LLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQ--LYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKD-GKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWR----KLRSQESSKN-HILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELI 2423
            G YV   D +QL +G+  G+  L+NL ++++  D  +LP+ V+ G +  L L +PW  L ++PV   ++  Y+L  P SE   + E+     F  K ++++  E     R                +    F + L  +I+ NLQ++I NIH+RYED     +T P+   +VG+TL  LS  + DS+     I  D T+    K + ++SL +YWN++  P  S   ++R+    +   K+ I+S         ++++P+S    + ++    T     P + L +  ++ G   +  +Y DI   L S+       +F+K RP+  +  N + W+ +    ++ E +  + +MW+W+ ++    + RLYT +W +KL  K L    +D I+  +          +E+ L      RQ A+ K            SS          +     S L+S  + +  ++V  +   T+E++ +LY AI + E         +P E V L++   L+K  F I     +   L  ++  +      QR  +        +++ Q+      DGS   L         S++ QS + N            LL   FE NPLD  A+  +NI+   + + Y    +  + KF     +    +  I     A++E     T  G+  L +     ++ +D++    ILP    +  D    ++D G + I+S+        +   +  ++  K++ + +    +D+F I+    Q  +   G D +    K+ +  S+ +  IL     E  +   ++     LP I V G LP L + I+D   ++++ LI
Sbjct:   15 GSYVENFDPNQLNVGIWSGDVKLKNLKLRKDCLDSLNLPIDVKSGILGDLVLTVPWSSLKNKPVKIIIEDCYLLCSPRSEDHENDEEMIKRAFRLKMRKVSEWELTNQARILSTQSENKTSSSSSEKNNAGFMQSLTTKIIDNLQVTIKNIHLRYEDMDGIFTTGPS---SVGLTLNELSAVSTDSNWAPSFI--DITQNITHKLLTLNSLCLYWNTDSPPLISDDDQDRSLENFVRGFKDMIASKNSTAPKHQYILKPVSGLGKLSINKLGSTEE--QPHIDLQMFYDEFGLELDDTEYNDILHVLSSIQLRQITKKFKKARPSFAVSENPTEWFKYIAACVINE-IHEKNKMWTWESMKEKCEQRRLYTKLWVEKLKLKNLEAPLRDPIQEAQLSELHKDLTYDEIILFRSVAKRQYAQYKLGMTEDSPTPTASSNIEPQTSNKSATKNNGSWLSSWWNGKPTEEVDEDLIMTEEQRQELYDAIEFDENEDKGPVLQVPRERVELRVTSLLKKGSFTIRKKK-QNLNLGSIIFENCKVDFAQRPDS-------FLSSFQLNKFSLEDGSPNALYKHIISVRNSSKDQSSIDNHATGEEEEEDEPLLRASFELNPLDGLADSNLNIKLLGMTVFYHVHFITEVHKFFKASNQHMETIGNIVNAAEATVEGWTTQTRMGIESLLEDHKTVNVSLDLQAPLIILP-LDPHDWDTPCAIIDAGHMSILSDLVPKEKIKEIKELSPEEYDKIDGNEINRLMFDRFQILSQDTQIFV---GPDIQSTIGKINTASSTNDFRILDKMKLELTVDLSILPKAYKLPTIRVFGHLPRLSLSINDIQYKTIMNLI 841          

HSP 2 Score: 116.316 bits (290), Expect = 9.249e-26
Identity = 134/500 (26.80%), Postives = 234/500 (46.80%), Query Frame = 3
Query: 8523 GVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSV---GIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLG--PEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEV---FRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDG 9998
            G+ L+Y  S        ++  +QIDNQ+   N+  +  P   P +   +    P+I  S   +         +K+  +LIQE  + LD+  + +++DF+      +  ++   K  + I+  L + S +KT         F+ FHI P  +HLSF  S     G      E  +  L  V    + + ++ +   K+      N  +P+  ++  I  HY +Q + Q++ ++   D  GNP G+   ++ GV DLFYEP +G ++   P+E    +A G  S     V G + ++S+  G+M KG+ ++T D ++QR RR Q      N    LA   +        G++G+   P    +K+G  GF KG+GKG+VG+ T+   G +D  S+  +G++    T +++    R+R PR++  D II+ Y LREA+G   W+K T       N+ Y+ HV    K+  + ++ Q I  +     +    + W   Y  + G
Sbjct: 2611 GIELRYNESKAYQTFSWKMKWMQIDNQLFSGNYSNILYPTEIPYTEK-EIENHPVISGSISKVNDSLQAVPYFKHVTLLIQEFSIQLDEDMLYAMMDFIKFPGSPWIMDSRDYKYDEEIQ--LPDVSELKT----AGDIYFEIFHIQPTVLHLSFIRSDEISPGLAEETEESFSSSLYYVHMFAMTLGNINEAPVKVNSLFMDNVRVPLPILMDHIERHYTTQFVYQIHKILGSADCFGNPVGLFNTISSGVWDLFYEPYQGYMMNDRPQEIGIHLAKGGLSFAKKTVFGLSDSMSKFTGSMAKGL-SVTQDLEFQRVRRLQQRINKNNRN-ALANSAQSFASTLGSGLSGIALDPYKAMQKEGAAGFLKGLGKGIVGLPTKTAIGFLDLTSNLSQGVKSTT-TVLDMQKGCRVRLPRYVDHDQIIKPYDLREAQG-QYWLK-TVNGGVFMNDEYLSHVILPGKELAVIVSMQHIAEVQ----MATQELMWSTGYPSIQG 3094          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Nematostella
Match: EDO34505 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SP53])

HSP 1 Score: 533.487 bits (1373), Expect = 7.856e-152
Identity = 355/935 (37.97%), Postives = 532/935 (56.90%), Query Frame = 3
Query: 7395 KAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG--WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQ--YNTPQ--------------ELILPAYQTVMYAWQDGRQKREFIWRVEGD--KKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAM---------------FVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSL----SRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNI 10082
            K  ++++ S WS KFSLDTVGS G +  KK    +E+GV+I LS   LTK+V F P+++I N +   +   E  +N+   W  V  GE +PFWP     K +++R     RE   +  F F    SIL+++E K   + VE+   + A ++    Y  G A +++ N    D+EI +KQ  Y                  E +L     ++Y W     K+ F+W  +G+  K +   +L   G   F + +   +W +F+    R+LL  +D  +A  A++    E I QEI+++L +IGLSLV+N    E+AYI IT S + W + ++K R K LK+  SE LE A+     +  TS         K+  +EVD     +I P +  + R F PGV ++   S H++ +  ++  +QID+Q+    F  V  P PPPRSVA+D   KP  E+S +  +   +     KYF+VL+QEM+ M+D GF+ ++++  S + P  D+  +               +++D + +   L   + ++  LS  +   FD FH+SP+KIH+SFS     +  +++G   + S  +NL +QSVG+A+T++QDV FKL YFE  N +    Q+  +I  HY  QA++QMYVLVLGLDVLGNPFG+I G+  G +DLFYEP +G + GP EF EG+A GV+SL GH VGGAAGAVSRI GT+GKG AAL++D  YQ++RR+ + K+P N+G GLA+GGKGL+ G  +GVTG+VTKP+ GAK+ G  GFFKG+GKG+VGVV RP  G+VDFASS+FEGI+  ADT  EV ++RPPR    D +++ Y   EA GN I   + +    +  + Y  H     KK ++ +TN+ I    + E+ G W   W   Y  L   P  +++K  +V  + +K R +F +  +
Sbjct: 2644 KTRVRMSGSEWSGKFSLDTVGSGGSIKIKKDDKDFEIGVQITLSYFSLTKVVEFTPFHLIDNHTEFPVSLCEAPDNSNLIWTTVKSGECIPFWPSRVPPKDLVVRLGDSTRE---SSRFNFEPDVSILLRMEGKVGALSVEMISKDSAAIMTFCPYYAGAAPIRIENCT--DLEICYKQDRYGVTNKVRGIPCHVSEGSTEHVLRPGNCILYTWGRPMDKKVFMWYAKGEDQKNRRITDLIGVGHGAFEVKDKSYFWATFLDGLQRVLLIIDDFTLAYRAQVEVP-ERISQEISLNLQAIGLSLVNNEKSIEVAYIGITPSQIIWEETKKKGRWKALKMRISEQLEVAYTRV--QQVTSVGGEPNFRQKLSDIEVDFELMEIIHPRRRAIRRTFTPGVAVKLIMSAHELELFTKVNSVQIDSQIPGSTFQTVLHPVPPPRSVAMDNAPKPFFEMSVLTRQVEHSHVNEIKYFKVLVQEMETMIDMGFIMALIELFSMT-PLIDDSGIHSSSTLGIVSDVLTQYIEDKKRVLEGLGAANAVQAALSDTRN-YFDYFHLSPLKIHVSFSQLGGPASDDKSGGIEIGSSFVNLLMQSVGVAVTEVQDVEFKLAYFEILNQVYNQQQLTGAIIKHYTGQAIKQMYVLVLGLDVLGNPFGLITGLKDGAKDLFYEPYQGLIQGPGEFAEGLALGVRSLVGHTVGGAAGAVSRITGTIGKGFAALSMDEKYQQERRQAMGKKPVNVGEGLARGGKGLLEGVFEGVTGIVTKPVQGAKEQGAAGFFKGLGKGVVGVVARPAGGIVDFASSTFEGIKGSADTATEVKKLRPPRVFYADKVMKPYNSYEAAGNAILSGVKKDKSALEEDKYYAHSILNIKKALV-VTNKHIIVAGKAEVFGNWECDWSCDYNDLTAEPEADKNK--LVLRVPDKGRVIFKRSEV 3565          

HSP 2 Score: 204.912 bits (520), Expect = 4.156e-52
Identity = 119/368 (32.34%), Postives = 201/368 (54.62%), Query Frame = 3
Query: 1377 KKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDN--SLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSK--LMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVP 2468
            K +  E  +EE AKLY AIGYSE       P EYV  K+ + L+++   + D+  S K  +++K  I  + A + QR SA AI V   ++ M++ G+ G  G +P +V++Q + +   M L    FETNPLD+  +Q++ + + PL+I+YD++T++ ++KF  PP+++ L E+S   ++ +E +K  +   L +  +    TDI +D+   Y ++PE+G       +L++DLG + I S+        D + +      L+   ++   YDKF I L  +Q L+ ++GDDW   R Q  SK HI+ P   +  L+K +  +D  L +I + G L  + V I+D+ L   ++L L IP P    P+  P
Sbjct:  498 KILTEEQKKEELAKLYAAIGYSENEVITSFPKEYVENKVIVHLKQIGVQLRDDCGSGKPQEIVKFSIKDLYADLAQRPSAQAIRVTAKVDKMKMYGSPGEGGHLPLMVTSQQEKEKGHMALFHAAFETNPLDEKCDQRVEVTSRPLKIVYDANTIDEVMKFFQPPQDVHLQELSAAAMSGLEALKTASKASLAYAVEHHKVTDINLDVMSPYILIPEKGCMQGSKSVLVLDLGHLKIKSDP-------DQERVITTK-NLSMAELESKCYDKFDIRLEQLQVLLAKEGDDWNAARQQTQSKLHIVNPISIDLKLQKALNPDDVRLAQIKLAGSLHSIDVHITDKKLDLAVQLALSIPVPGGDTPQDKP 857          

HSP 3 Score: 135.191 bits (339), Expect = 4.214e-31
Identity = 139/679 (20.47%), Postives = 310/679 (45.66%), Query Frame = 3
Query: 3441 ITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAI-------DQSTSVVLEESKITLSNLRVSRSILLE-NTVKNEKGILHPINIFMDMTRNLS-WNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEA---INQVPSVPESKEEVKSRKILPK-DDKS----GSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDK-CLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPV-RGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDN--FWEKREFDENEFLFIS 5414
            + A L  + ++V + K  +++ + + G+ + +      T +   +TD+ + D  P  +Y  I+ I   E +FS+Q+ ++N   +  G   ++  VD    + +G I++IFL   V+ +  FL++FQ++ + +    +  +E AA KI ++  +  ++R KL ++++AP I IP NS S +AL+ DLG L   N F +       D S +V+++   + LS++++SR+++ E + +     IL P+N+ + + R+LS W   H IP I +   + ++ +   + D +    V+  N  E    I   PSV  S E      I+P+  D S     S+  A++   TV+F FK+E +   L+        W       + CLT       +V G       ++ ++R++    +   +     ++  I   +  +++ + +KF Q  + + ++ +D+  +++   +++L++L + F  A       P    V   +   K            P +    +  +   V  PEI+L+ D  +  ++A+ ++        + +    +   I +       F  ++R      ++  + ++L+ + P    +   +      ++++PA I++++  + +L                ++++ +I D+   W ++E D+    +++
Sbjct: 1045 LKAKLGGISLVVTSVK-GDLVRVLVGGLSSHISVMEDRTSIDAKMTDLSVVDAKPDVLYPNIVSIVNQE-VFSVQIVVYNDAMAG-GNFTNMDAVDTSFEMSIGCIRVIFLNKIVMELLAFLDNFQKAKEAMEYARKSATEKAAEKIQDIQSQ--AARIKLTVSIQAPFIIIPVNSHSRDALVADLGQLVVSNSFKLHSPATKPDGSDAVIVDNMVVELSSVKLSRALIGEGDQIVASSVILEPLNLTIYLARSLSAWY--HKIPGIDVTGELCAVKMCTGELDLSTILAVIFQNLGEGQSDIQDEPSVESSGEGA----IVPEVGDGSVAVVPSSTPATEVYDTVKFDFKIESVSAVLY--------WKESTELLRICLTN------TVLGQAK-GVILQCNLRMAQ-KTIKGHVGYFIMMDRFI-GAEGKAANMVDVKFDQSASLSKQITIDVNSVHVVANLEFLLVLANVFTTALTEAPPKPPSVSVETSQALTKVKPSALAPAPPPPPDKEPPEMRLAFSVKHPEIVLLADAKDKATHALFLKNTLEFNLIMAEGQQKMFGYIKNLNITSAAFDLKNRAATKSQVLYLSQLALHGSAPEGECLHIDVRTSIAQVHVSPATIKTMTACLMSLAPAQGQ----------SDKNNVIDDDVTLWSEKEIDKKSKWYLT 1685          

HSP 4 Score: 119.013 bits (297), Expect = 3.921e-26
Identity = 148/620 (23.87%), Postives = 259/620 (41.77%), Query Frame = 3
Query: 5511 RADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLE-VYPEEHNRQYSFEIEY-----------ISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFG-APYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYL--SIYRTEVRALLFS--PKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYY---------YSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHD-----------IKPGCLELVR---------------------------------TACL-------KVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFK--AKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYK 7133
            ++ Q      ++DE +   +   M    + +E     + VP+L  +  +  ++K+  +++     +S+  +Y+N++++ WEPL+E V  +E NR +    E+                +G  D       ++ EP A+S++L S D L++T++KS LDV + LG AF +A SL      +I   +P+VV+N  G  +  ++     LKP    EE  + +     + LY A  EK++     K  +      A  +   +   +I   + R   +S  PK      RG        + +V   +  G + + +RS LQ+ N+    + L         SE +K   +         Y+VP+  +L+    +++         + S  LWN     + + T   L ++N    +Y         Y+V++ +E    V    T   Y      P+ +HN LP  I + SQ+  +D           ++PG L L +                                  A L       KVQ +IK+   Y   +W G ++L   M+EL+       AK+ +    ++LGV  +  +G  NVT+YSPYWM+NKTG  L YK
Sbjct: 2065 QSSQHHIEAGSIDEERGEMLLFSMEGFSIKMEGAYGGQTVPLLSMDGHIQAEVKDWSSKLTASAGLSLIANYYNENVSEWEPLIEPVECQEKNRPWELSAEFKMGDEVGGGENGEEPSAGGLD-------VNSEP-AMSLVLESHDDLQITITKSGLDVFNKLGAAFSEAVSLRGGDMVAIEDMSPFVVRNEIGLGIKVHLGS--GLKPPPDHEEKALPVPVGKSLSLYLADPEKRASFKGVKGDEASDISIAVEVDGYHPIKNIPLKQARVAFYSIVPKQL---LRGTTM-----SAVVQIESGEGQRVITVRSPLQIHNHFPVPMDLCC----KQGSEVSKVTCIAPH----SVYDVPL--ILSYRAGLFLKPSGFGYNETTSPLLWNDLSQTKKA-TFTCLSQTNGEPPFYIEVHCEKAVYTVSVGMEANSPVF---TFHAYP-----PVVVHNYLPYSIHFSSQVSRNDGARQGDGGAVLVEPGKLALYKQFSRYSSKLTIKLLTPSVVHLLGTPFVNLEGGHNAPLYSVDMGKKVQLHIKLN-AYMGMDWKGDIELSRDMDELTTFTLSPTAKDTHLRP-VELGV-FSQNDGTMNVTLYSPYWMVNKTGMFLEYK 2644          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Nematostella
Match: EDO31907 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWL5])

HSP 1 Score: 268.855 bits (686), Expect = 1.487e-75
Identity = 168/516 (32.56%), Postives = 275/516 (53.29%), Query Frame = 3
Query:   27 VFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKR----NDPKKETETKDSFGEKLAAQIVKNLQI--------------------SISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFS-SENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHM-KPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDT----IKVCEEELDLLNITLCRQQAEMKA-KKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDE--LQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIE 1475
            +FE LVV ++N  LG Y+  LD S L+L +L G A L +L+++++A D FDLPV+V  G + +++L IPW +LYS P    ++ +Y++  P  E  YD EK R+     ++K L +IEE+  K+     D     E  DSF EKL AQ++KNL+I                    S+ NIH+RYED  T     FAVG TL NLS +T D +  + ++   + +  FK V +D+LAVYWN +   +    N    K  M++ IS+  ++  D  +++QP+  SA  HV M +       VP++ +++  +++  A ++ Q+ ++   +DS +      +++KY+P V +  N S WW + Y  I EE +R     WSW+ I+AHR R R Y  ++++KL  K    SE+++    ++  E++L + ++ + +QQAE++   +MR+ L+                 T + S  +  G +E  L K       ++EK KLY+AI YSE       P+E
Sbjct:    1 MFEQLVVSVLNSVLGRYIRDLDSSNLELAVLQGQAVLRDLELRDDALDGFDLPVQVTHGFVGEISLTIPWTNLYSSPCDICIEDVYLIASPVKEQPYDAEKARASELAVQRKRLRQIEESLAKKATESTDGIAHAENGDSFVEKLMAQVIKNLKISPVQFIEERTENTLAALTTVSVKNIHMRYEDQVTSCESPFAVGFTLQNLSAETTDDNWKTSVVNA-AAKTVFKLVSLDNLAVYWNPDQHAYDLYGNHWNWKGKMEDGISTKAKQPKDYEYILQPL--SAECHVIMDQTSKVEMDVPKLLVEVIQQELSLAMSQKQFHNLMDVIDSFELMSVNQQYKKYQPTVKVHGNASKWWKYAYTAICEEGIRS----WSWERIKAHRARYRAYKSLYKRKL--KGFHDSERESSLRHLQALEDDLGIPSVLIAKQQAELEVPPEMREVLSPAK--------------TSSGSWWSWLGGEEDLLSKLTVEALPKDEKEKLYKAIDYSEGARPIKYPLE 493          

HSP 2 Score: 62.7734 bits (151), Expect = 2.725e-9
Identity = 50/163 (30.67%), Postives = 76/163 (46.63%), Query Frame = 3
Query: 1359 GSDELQKKVRSE-FTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEY--KLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDG--SVPTLVSTQAQ--SKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDT 1826
            G ++L  K+  E   ++EK KLY+AI YSE       PIE  +             +     +E+  K+ ++     +A ++QR   DA+     +N  QV GT  P    S P L+S++    S    LL    ETNPL   A   + ++ EP+EI YD+ T
Sbjct:  518 GEEDLLSKLTVEALPKDEKEKLYKAIDYSEGARPIKYPIEVGTTGR----------VQGRDYREFARKIAEVATEGFAAAIEQRPGGDAMRFQAEVNGFQVLGTPNPSDCQSRPCLMSSKTSLGSDGKALLSFTAETNPLHVCAEYAVALKVEPVEIRYDNVT 670          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Nematostella
Match: EDO31906 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWL4])

HSP 1 Score: 180.644 bits (457), Expect = 3.484e-48
Identity = 108/309 (34.95%), Postives = 163/309 (52.75%), Query Frame = 3
Query:   27 VFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKR----NDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSS-GTSII--------------------------------------IKEDSTREFFKSVKMDSLAVYWNSNPKPFS-SENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHM 821
            +FE LVV ++N  LG Y+  LD S L+L +L G A L +L+++++A D FDLPV+V  G + +++L IPW +LYS P    ++ +Y++  P  E  YD EK R+     ++K L +IEE+  K+     D     E  DSF EKL AQ++KNL++S+ NIH+RYED  T     FAVG TL NLS +T D +  TS++                                      +   + +  FK V +D+LAVYWN +   +    N    K  M++ IS+  ++  D  +++QP+  SA  HV M
Sbjct:    1 MFEQLVVSVLNSVLGRYIRDLDSSNLELAVLQGQAVLRDLELRDDALDGFDLPVQVTHGFVGEISLTIPWTNLYSSPCDICIEDVYLIASPVKEQPYDAEKARASELAVQRKRLRQIEESLAKKATESTDGIAHAENGDSFVEKLMAQVIKNLKVSVKNIHMRYEDQVTSCESPFAVGFTLQNLSAETTDDNWKTSVVAAAAKTVFKVTSCESPFAVGFTLQNLSAETTDDNWKTSVVAAAAKTVFKLVSLDNLAVYWNPDQHAYDLYGNHWNWKGKMEDGISTKAKQPKDYEYILQPL--SAECHVIM 307          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Nematostella
Match: EDO42077 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2Q1])

HSP 1 Score: 166.777 bits (421), Expect = 4.604e-46
Identity = 94/187 (50.27%), Postives = 130/187 (69.52%), Query Frame = 3
Query: 9039 LNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDG 9599
            ++ F+ S+G  + DM D   KL +FE+    +    +   IT HY  Q +RQ+Y LVLGLDVLGNP+ +I G+ +GV DLFYEP +G + GP+EF EG+A GVKSL GH VGG AGA SRI G++G+ +A LT D+DY+ KRR+++   P+ +G GL  GGK L++G V G+ GVV KPI+G K++G
Sbjct:    1 MDFFMDSIGATLIDMNDAQLKLAFFERVGVFITQENLQGQITRHYTMQGVRQVYALVLGLDVLGNPYALISGIGEGVRDLFYEPYQGIIYGPQEFAEGLARGVKSLLGHVVGGTAGAFSRITGSIGQAVATLTFDSDYRMKRRQRMHVEPKGVGQGLIMGGKSLVMGLVFGIGGVVMKPIEGRKEEG 187          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Nematostella
Match: EDO41450 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4C6])

HSP 1 Score: 174.866 bits (442), Expect = 4.920e-43
Identity = 121/422 (28.67%), Postives = 212/422 (50.24%), Query Frame = 3
Query:   27 VFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIP---NSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETK------DSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQ-TCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEE-QVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKK 1259
            + E L   ++  Y+G+YV  L+  QL +G+  G   L NL +K+ A    DLP++V+ G I  L L IP +   SEP I  +D LY++  P   N+E     E ER ++ + +Q +L    E++ + +  K +T          S     +  IV+NLQ+ IS++HIRYED S   +  FA GIT+  L+ Q T D   T  +I+ D + + +K + + + A+YW+S+           L+  ++  I  S   +   S++++ IS SA +  +          PR  +D+ +E+I  +    QY      +   +R + + R+ K+RP VP+++N  +WW F    IL   Q R R    S+   +AH     +Y  ++ + L+  +L  ++++ ++  E+EL    + + R+ A  KA+K
Sbjct:    1 MLEGLAAWILRTYVGEYVENLNTEQLSIGV--GTVDLHNLPLKKTALQGLDLPLEVKSGFIGHLQLSIPLRRPKSEPWIINIDKLYLVAGPPLHNNEYNEQQEAEREWIRKKRQLDL---LESQWQESQQKGQTGGFWSGSWWPSLYSSFSTTIVENLQLVISDVHIRYEDASIDQHTPFACGITIDRLAAQSTNDKWETEEVIQNDHSIK-YKLIDLQNFAIYWDSDAPLVGDLPSTKLEVALQRTIDKSSGSIGKHSYILESISASAKLQRNATALPLRDKNPRFVVDVHVERIPLSLAEVQYRMGMKLMGEFERFFRQARYHKWRPKVPVKNNAKLWWQFASTCILHFIQERNRHHTLSFATKRAH--DLVVYVKLYTEHLMSNQLESAKKEELERIEQELSFEELAVLRRLAMRKAEK 414          

HSP 2 Score: 79.337 bits (194), Expect = 3.774e-14
Identity = 115/447 (25.73%), Postives = 203/447 (45.41%), Query Frame = 3
Query: 8532 LQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLK-PLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVS--------GSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFR---IRPPR-FIAKDGIIRSYGLREAEGNNIWIKITE 9833
            + Y  +P    +   I +IQ+DNQ+ +   PV+  P    +    +   + P + +SA    +       YK   V ++ M L +++  +  +L F           +    D+E ++  D Q  RA++  +          ++  F+   I+P+++ +S     V   G+    SE L     S+G+ + +++D    L  F + +    +N  + S++ HY  +   Q   +V  +D LGNP G++  ++ G+E L     RG V G       VA+GV             + ++I G++  G+   ++D  ++  R    A+R  +       G +GL +G V G+T +VT+PI+GA   G+ GF  G+ KG+VG V +P +GV+DFAS +   +R  A  +   F     RP R     +G I  Y    AEG  I +K+ E
Sbjct: 4368 VDYVRTPKIETLEANIGRIQVDNQLFNSTLPVLLYPTAAEKGSREEHATQQPTLHISASKEPTRIQNVEIYKGVEVTLRRMTLHVEERLLLKLLQFFGYVQKDDDLATTDEEDDECLY--DNQ--RAAVTSSG------PASRRYYFERLQINPMQLKVS-----VASAGRL---SEDLASLKSSLGLILFNLEDATLDLESFIRDHPFESLNSHLDSMSKHYTEELRSQTAAIVGSIDFLGNPMGLLNDVSSGLEGLVK---RGNVGG---LFLNVAHGVSD-----------SAAKITGSLSDGLWNASMDTKFKESRETMRAERYGSSRDHFMAGMRGLGMGLVGGLTSIVTQPIEGAHNAGLSGFMTGLAKGIVGTVAKPTAGVLDFASGTAAAVRGQATRSSRYFPPKCTRPRRNCFGPNGSIPRYSRTHAEGQEIMLKLNE 4779          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Medaka
Match: vps13a (vacuolar protein sorting 13 homolog A [Source:NCBI gene;Acc:110014158])

HSP 1 Score: 567.385 bits (1461), Expect = 5.978e-162
Identity = 361/1055 (34.22%), Postives = 589/1055 (55.83%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLK---RNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPET-TNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQE-EKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQ--AQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSE-RTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQV-LRAINVLPDSETASLTSTIXXXXXXXXXFKD---ALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIP 3152
            MVFESLVV+++N++LGDYVV LD SQLKLG+ GG+A L++L+IKENA  + D+P KV+ GHI +L LKIPWK+LY++ V A LDG+Y+LI+P + +KYD EKE   L E +Q+EL RIE+ K K   ++DP++E +  DSF EKL  Q++KNLQ+ I+NIH+RYED                N+S QT D +    ++ E++ R FFK VK+++L  YWN N   FS+++       ++N+++  +    +  F+I+PIS  A +   M P +  +F+ P++ L + L ++    N+ QY  I   LDS+D       +RKY+P V +  N   WW +    ILE  V+    MWSW++I+ HR   + Y + ++ K+  KK S +    ++  E+ LD+ NITL R+QAE++A K   ++ +               +++   S   +   E QK+ ++ F     KAKLY AIGYSE   +P++P ++  +K+N  +++L   I D   K  ++M+L +  + + + QR  A AI +   ++  +VTG    +   PTL+S++  A      LLI+ FETNPLD+SA+Q+ ++ ++PLEIIYD+ TVN + +F   P+++QL E +   L  +E+ K+ TATGL ++ + +   D+KV++  SY I+P+ G Y     +LL+DLG   + S+ + ++P               N + +   AYD F + LS++Q L  + G DW+K R Q+ S  HIL P     +  + ++  D+ +PK  V G LP L + ISD  L++VLEL+  IP      P+  P+  +     + K   + P L+     +     ++  D    + +S + +D      F D    L  +D+    +++   K    KNM +    F+IN F +Q+ +      + +     ++ L +  +GT L L+ FDM    Y+  + L  P++VD   K  V L  T  +++  +LL +  +  +K  P+F ++H NT   ++  F S++V LH EALL  +NF+++L+P
Sbjct:    1 MVFESLVVDVLNRFLGDYVVNLDSSQLKLGIWGGDAVLKSLEIKENALSELDIPFKVKAGHIGRLELKIPWKNLYTQSVEATLDGVYLLIVPTASIKYDAEKEEKQLQEARQRELQRIEDTKRKAAEQDDPQQEKQ--DSFMEKLVTQVIKNLQVKIANIHVRYED----------------NVSIQTSDKNWKPSLLDENA-RLFFKLVKLENLFAYWNVNSVLFSTQSAEEALRSLENSMTIQKSPAANHDFIIRPISADAKLK--MNPRSDVDFSSPKVDLMVNLSEVAIELNKPQYISILELLDSVDMMSRNLPYRKYKPVVQVHQNAGTWWKYCITSILEVNVKPHLHMWSWQHIRRHRATVQCYKEFYKTKITCKKPSDNLLKDLENTEKALDIFNITLARRQAEVEASKAGLRIYRPGVKMEEE-------ESQGWFSWVWNWGGE-QKETKNNFGNIISKAKLYTAIGYSETAANPNLPKKFEDMKVNFHMERLCVSIKDEKDKN-EVMRLTVGELKSTLTQRPGAQAIKITAELSLFEVTGL-ASNRPAPTLLSSRRAATKTGTPLLIILFETNPLDESADQRFHVSSQPLEIIYDALTVNSVSEFFRLPDDVQLNEFTKATLMKLEQFKDRTATGLLYVIETQKVLDLKVNLMASYVIVPQSGFYDGTQNILLLDLGHFKMASQSKENLPQLTPC--------PSNIEAIMSRAYDTFDVQLSSLQFLYSKPGGDWKKARKQKQSPLHILEPVDVNVDFSRAMVVKDSRMPKYKVLGELPLLSLRISDIKLRNVLELVDSIPL-----PESKPSAHNTAPSSTQK---VRPALSQNRKSLKFSEFDLTEDLFYDAPSSPVGDDSF----FPDNPLPLRYVDSR---NRRSLIKEDVQKNMTEFLMTFLINEFSVQLCR------MSEGQEVTVLHLDIEGLGTELQLRTFDMTSNTYLREICLTCPEYVDMEQKQ-VQLITTLDNSE-DDLLTLEYIKADKNGPEFKSLHNNTEQMIKVTFSSLDVHLHTEALLNTINFLSNLLP 993          

HSP 2 Score: 522.316 bits (1344), Expect = 3.555e-148
Identity = 369/930 (39.68%), Postives = 534/930 (57.42%), Query Frame = 3
Query: 7368 KNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLY-CNELVENAGWIKVPPGE-AVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPF---YESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELI----LPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNL-TSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKW-NQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVE---EHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSG-SVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTG-KTALHSEV-----LNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEI 10094
            K + F +  K  L I++S  SD+FSLDTVGS GKV CK     Y VGVKIN SS  LT+IVTF+P+Y ++N++   ++ C +  E   W ++ P + A PFWP +   K   ++ KV+     +T P       + +S+L+ L     GI V+V ++E    I    Y++G A   +INH  D   ++F Q  + Q+ I    L A + V Y W D    RE  W        +S  L T + + D   +E K + +SF     R+++FT D  +      S  +++ +QEI + L ++G+SLV+N+  QE+++I IT S V W +Q R+K R K L + + + LE  F E++N     +A VE    + V+     VD+    M++P    L R F PG+ ++Y  SP Q    ++I++IQI NQM    FP VF P   P+S+ +D+  KPLI++  V   +G +   R KYF+VLIQEM L LD GF+ SI D V+  +  +     +F KD + I+  L + S   T        +++ FHISPIK+H SFSLS     G K    +EV     LNL L+S+G  +TD+QDV+FKL  FE +       Q+   +  HY  QA++Q YVLVLGLDVLGNPF +IRG+++GVE  FYEP +GA+ GPEEFVEG+A G K     AVGG AGA  ++ G M KGIAA+T+D DYQ+KRRE + ++P  L  GLA+GGKGL+ GFV G+TG+VTKPI GA+K+G  GFFKG G+GLVG + RP  G+VD ASS+F+GI+R A+ +  V  +RPPRFI +DG+IR Y  REA G+ +  KI  +        Y  H    ++ + + +T + IF + R    G  + +WQ  +E+    P I+  +  +    KE+ + +F      K I
Sbjct: 2228 KPRHFLRNNKVRLMISESELSDEFSLDTVGSHGKVKCKGIHKDYLVGVKINASSFSLTRIVTFVPFYTLVNKTKYKIFICED--EGDTWTEINPEQPATPFWPENGTKK---LKIKVDG---CLTPPLKVDFTRQENSLLLHLNDSLGGIIVDVNLSEHLVTIQFSEYRDGSAPFLIINHTNDQT-LYFNQ--SSQKEIEKEELEAGKAVYYTWTDPTGSRELCWTC----GPYSGTLKTEEDLHDDMKDERKLFVISFFEGLQRVVVFTEDQDIYNLLGESEKIQAAEQEIILLLQNVGVSLVNNTTSQEVSFIGITCSDVVWESQPRKKKRWKILNIKEGDMLEKHFKEYINSSPIEDAIVELENNYKVRFTSNGVDMR---MLQPFDAPLRRHFLPGIKVEYSISPRQSTYRVKIHRIQIQNQMQSAIFPYVFYPVKLPKSITMDSEPKPLIDICIVTRAAGHSEIPRIKYFKVLIQEMDLKLDLGFLYSIFDLVNPENTTSMSPVELFEKDIEYIKTDLNDVSAADT----SPISLYEFFHISPIKLHFSFSLSSGGEDGSKEKRATEVIPVQSLNLLLKSIGATLTDVQDVVFKLPAFELKFQFCTTQQLQWKVIRHYSKQAIKQAYVLVLGLDVLGNPFALIRGLSEGVEAFFYEPYQGAIRGPEEFVEGMALGAKVFVDKAVGGLAGAAFKMTGAMAKGIAAITMDEDYQQKRREAINRQPSGLKEGLARGGKGLVSGFVSGITGIVTKPIKGAQKEGAVGFFKGCGQGLVGAIARPTGGIVDMASSTFQGIKRAAEASQNVESLRPPRFIHEDGVIRPYKAREANGSQMLQKI--ENGRFAKYRYFAHANI-NESDFLMITKRGIFFVTRG-TFGQLTCEWQYLFEEFTMDPRIDGGRR-LRIEAKERVKSVFHAKEFGKII 3130          

HSP 3 Score: 258.84 bits (660), Expect = 4.988e-68
Identity = 228/902 (25.28%), Postives = 421/902 (46.67%), Query Frame = 3
Query: 3441 ITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFA-----IDQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNE---AINQVPSVPESKEEVKSRKILPKDDKSGSARTASDE-----SITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIK--NDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHN---RQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDL 6092
            I A L+ L+V +  +K   I +I + G+ ++V       EVS  + ++ + D +  A YKK + I  D+ +F+ ++  +   T   GY L++S VD  V + VG I++IFL  F+  I  F+N+FQ + + +A    + +E AA+ +  + E   S+R  L+++  APVI++P++S S N ++ DLG L+ KN F       D +   V+++  + LS+L++ R+  +    K E  +L P+++ +++ RNLS N  H IP I I+A +  +S+ + Q D  +    L  N +E   A++ +P +P +KE   S       +   +A     +       T++  FK + + V L   + N  E          L +     +S    M  +S+M+  V+L+    LD +  S K +   +  ++   + +  + + + Q  +  +   V +  +Y+C  +++L+ + D F+KA               +G+ + P+  +     SPV    +  E++V V  PEI+ V D+   D+ A+++ T   L      +  ++   I D Q + CPF +  R  N   ++ P  +    ++  +      +++  L + ++P +I ++  I + LT  +      R          L+    WEK+ + E +  F+                               +  +D+   +   L    ES + + +  I + LE+ V    VPML+ + S DG++KN  T I L     + V Y+N+ + VWEPLLE   +E     R ++ E++ ++  D  + DE DF    ++      I+  S+D L +T++   L +LSNLGKAF +A     +  +    AP+VVKN  G  V   ++K F L
Sbjct: 1028 IRADLRCLKVFLRGQK-ARISEIRVEGLVSEVLQRKKMMEVSANLKNIVVLDCNKEASYKKAVSIA-DKEVFAFRMLNYTDATEGDGY-LNMSNVDTSVTLTVGCIQVIFLNKFLDSILAFINNFQMAKEAIAEVTVQAAEKAASGVKELSER--STRISLEVHFNAPVIFLPQSSSSSNVIVADLGKLSVKNRFVKQPFKSDSTIPPVVDKMTVKLSDLKMYRTTYISGIFKGESQLLKPVSLDLEIQRNLSSNWYHNIPDIEISAHLKPMSLILCQDDMIVVLRTLGENLSEKSDAVSPLPDLPSAKESQNSGASGSDSNIVVTAAVVETQKYVKLKTTLKLEFKFDSMTVVLHSPNKNEMEMLDLHHEQLKLAEFTLGTISTSVQMFSDSSMKAFVQLTACQ-LDDKRPSIKMVTSRMVAMRPGAEQNTMMEVNYRQGHDGTTIDTV-VQDLYLCASMEFLLTVADVFLKATQ-------------QGFAQGPQA-KSTAGTSPV----SKTEMNVVVRNPEIVFVADLSRADAPALVMTTQCELDMKNGADGQMMTAIIKDLQVMACPFLTSKRGNNMTTVLQPCQVFFQSSQHPDAPQVMEVSINALSLKVSPIIINTVITIQSALTPTTETHEEER---------SLVPVKLWEKQSWKELKMWFL-------------------------------EENSDEDTKSLVPLIPKGES-LQVTINSICLTLEAGVGHHTVPMLLAKSSFDGNVKNWTTLINLKSKFDLEVHYYNEMMGVWEPLLEPLEDETGEDFRPWTLELKPMNLSD--IADEVDF----NLPDYKTVIVFNSKDQLNITLTNCGLSMLSNLGKAFSEAAKQTADSFQKD-EAPFVVKNMLGLPVSLQLSKRFHL 1856          

HSP 4 Score: 65.0846 bits (157), Expect = 1.834e-9
Identity = 45/143 (31.47%), Postives = 74/143 (51.75%), Query Frame = 3
Query: 6795 LHNQLPIKISY---QSQIFSHD--IKPGCLELVRTACLKVQSNIKVTY-HYQKKEWFGSLDLGPKMEELSVINFKA----KEGYSESF------MDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANT 7175
            LHNQLP +I+Y   ++ + + +  +KPG    + TA +  QS +++    Y  ++W     +    EE+S I F++    ++ Y E        +D+ VH+   +G   + I+SPYWM+NKTG  L Y A D   + H  A T
Sbjct: 2078 LHNQLPYQITYRLKETGVSAPEAILKPGHSTQLHTAVIN-QSQLELRLIDYLSQDWSSEYSISSNQEEISFIVFQSLKEEEDEYVEGTERKKAELDIAVHVKDDSGHTAIAIHSPYWMMNKTGRLLQYNADD---IHHKHAFT 2216          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Medaka
Match: ENSORLT00000000640.3 (pep primary_assembly:ASM223467v1:3:5428377:5447491:1 gene:ENSORLG00000000514.3 transcript:ENSORLT00000000640.3 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 412.149 bits (1058), Expect = 1.396e-122
Identity = 324/834 (38.85%), Postives = 468/834 (56.12%), Query Frame = 3
Query: 7368 KNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAG--WIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFY-ESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSN--NLTSDGIEDF-YINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKK--LMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFR------VLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRT---GKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIW 9818
            + +K     +  L I+ S WSD FSLDTVGS G   C +     +VGV I +SS  LT+IVT  PYY ++N+SS  L   E+V  +   W  +   E +P WP +  GK  +     E      ++ F F+ E    L+KL+    GI + V  +  + +I+   Y EG A   L+NH      I + Q  +     L   +   + W D  + R   W       +HS   NL  D +  F Y N+ + +W+SF+    R+LLFT D  V   AR + +LE   QE+ +SL S+GLSLV+NS + E+AYI I +SGV W +++ KNR KP      E LE A+     +L +++       V     +V+LS+   +M KP  C + R F+PG++++ + S HQ  +  +++ +Q+DNQ+                                        GAM    F        +++    ++    V ++  F SG +           D Q ++A L+E S  +T  SG     F+ FHISPIK+HLS SL   + +      AL S  LNL L+S+G  +TD+ DVIFKL +FE +       +++     HY  Q L+QMYVLVLGLDVLGNPFG+IRG+++GVE  FYEP +GAV GPEEF EG A GV+SL GH VGGAAG VS+I G+MGKG+AA+T+D ++Q+KRRE++ ++P++    LA+GGKGL+ G V GVTG+VTKP++GAKK+G  GFFKG+G+GLVG+V RP  G+VD ASS+F+GI+R A+   EV ++RPPR I +DGIIR Y L E++G +++
Sbjct:  107 RRKKLFTENQVKLCISTSSWSDGFSLDTVGSYG---CSRSGSILQVGVSIQVSSFNLTRIVTMSPYYTLVNKSSYELEVGEVVNASSTKWHYISSTECLPLWPENAQGKLCVRVVGSESS----SECFFFHREDRGTLLKLDCG--GIILNVNGSNHSTVISFSDYYEGAAPALLVNHTAW-ATIAYSQSGSAVVHELKPSEARKFVWDDPTRPRMLRW----SYMEHSGELNLLKDEMGQFSYDNKSQIHWISFLDGRQRVLLFTEDTGVVTKARQAEELEQSQQEVRLSLQSLGLSLVNNSSRHEVAYIGINNSGVVW-EMKPKNRWKPFSQKNIELLEKAY-----QLNSTDLTCCLWFV----YQVNLSRAGMMMRKPYSCPIRRTFQPGIYMELKQSLHQRSLRAQLHWLQVDNQLP---------------------------------------GAMFPIVFHPVPPPNTMLKRHPFLVTNSEVTTMFVFQSGLIEA---------DLQGLQAELMEASLSET--SG--LSFFEYFHISPIKLHLSLSLDSSSNSMDQETAALQS--LNLLLKSIGATLTDVDDVIFKLAFFEVKYQFYRREELMKEAARHYGEQFLKQMYVLVLGLDVLGNPFGLIRGLSEGVEAFFYEPFQGAVQGPEEFAEGFAIGVRSLLGHTVGGAAGMVSKITGSMGKGLAAITMDKEFQQKRREEMNRQPKDFSDSLAKGGKGLLKGVVGGVTGIVTKPVEGAKKEGAAGFFKGIGRGLVGIVARPTGGLVDMASSTFQGIQRAAEATEEVTKLRPPRLIKEDGIIRPYDLTESQGFDLF 862          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Medaka
Match: ENSORLT00000031398.1 (pep primary_assembly:ASM223467v1:3:5374961:5398684:1 gene:ENSORLG00000029522.1 transcript:ENSORLT00000031398.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 377.867 bits (969), Expect = 5.395e-113
Identity = 238/715 (33.29%), Postives = 391/715 (54.69%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFD------DFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKET--------------ETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDT---IKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDN-SLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSM 2096
            MVFE+LV + +N+++GDYV  LDKSQLK+G+ GG+            FD      +FD+P KV+ G I++LTLKIPWK+LY+E V+A ++GLY+L++P + +KYD  KE  Y  E KQKEL RIEE  L+    + E+              E KD+F EKLA Q++KNLQ+ IS+IH+RYED  +          +  N     C +      I  ++ +  +K   ++ L  YWN N   F  E+   +  ++   + S +       F  +P+   A + ++   E    + P+++L L+++ I F   + QY ++   L+S DR      +R +RP+VP+ +N  IWW +  N I+E  +R    MW+W  I +HR   + Y  +++ K+L +  S++  DT   I+  E+ LD+ NITL RQQA+M+  +  +KL  K ++G    G F        +        E Q+   +  T EEK KLY AIGYS  + +  +P +YV++ +  +L +    + +   + E  ++K+ + ++S  V QR  A A  V + + +  VTG +   G VP+L+++   S    L  V FE NP + + +Q + + ++P+EIIYD+ TVN +V F    + + L  +++  L+ +EE+KE TA GL H+ + R   D+ +D+KPSY +LP+ G Y+   ++++VD GS  + S  + +
Sbjct:    1 MVFEALVSDTLNRFIGDYVENLDKSQLKIGIWGGDG-----------FDVFVFQSEFDIPFKVKAGQIARLTLKIPWKNLYNEAVVASMEGLYLLVVPGATVKYDAAKEERYQQEAKQKELRRIEE-VLQVATSRSESLLLKFLVVSDTPKAEKKDTFVEKLATQVIKNLQVKISSIHLRYEDDVSTDQG------SDCNTCLMICFNENWKSCILNEAAKIIYKLGSLECLCAYWNVNSVMFYKESWENI--VVSLIVESPQWPFLFTVF--RPVFAFAKICINPNAEL-ELSSPKVNLHLEVQNIAFEMTKLQYLNMVELLESFDRMVKNAPYRMFRPDVPVLNNARIWWKYSINSIMEVHIRHLSHMWAWSNILSHRRNLKAYKTVYKNKILAQ--SKTGPDTERQIQDLEKVLDVFNITLARQQAQMEVIRSGQKLAAKKAAGQKQGGGFFSGWFGKKTK------KEDQEFEENLMTPEEKEKLYTAIGYSGSSHNLTLPKQYVAVVVTFQLLRTSLTLREQPDIPE--IIKVQMINLSTKVSQRPGAQAFRVEMALEHWFVTGLQ-QQGDVPSLIASVGDSSSSLLS-VVFELNPEESTGDQLLRVHSQPVEIIYDALTVNSMVDFFKTEKFVNLEVLTSATLSKLEEIKEKTAAGLSHIIETRKVLDLMIDLKPSYLLLPKSGFYSATSELVVVDFGSFQVSSRASCL 680          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Medaka
Match: vps13d (vacuolar protein sorting 13 homolog D [Source:NCBI gene;Acc:101163959])

HSP 1 Score: 161.384 bits (407), Expect = 1.563e-38
Identity = 115/393 (29.26%), Postives = 198/393 (50.38%), Query Frame = 3
Query:   27 VFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETT-NFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEE 1202
            + E LV  ++N YLG YV  L+  QL + LL G   LENL ++++A  +FDLP +V+ G I K+TL+IP+   +S+P +  +  L ++I P    +YD EKER    E K K L +  E+K K    +K      S    L  +IV+N+++ I ++H+R+ED S+     F+ G+ + N+S Q         ++++       K +++   +VYW++  +         ++  M   + + E +     ++ +P+  SA++  +   E   + + PR+   ++LE +    ++ QY  I  FL  +DR      +RK+RP VP+ +N   WW F     L   +R +RR  SW +        R YT ++  +L    LS  E+  +K  EEE
Sbjct:    1 MLEGLVAWVLNTYLGKYVSNLNTDQLSIALLKGAVELENLPLRKDALREFDLPYEVKAGFIGKITLQIPFYRPHSDPWVISMSQLNLIIGPAPPQEYDEEKEREDENERK-KHLLKALEDKFKCECEQKGESYWYSVTASLVTRIVENIELKIQDVHLRFEDDSSNLENPFSFGVCINNVSAQNPAKEMVQKLLRQ-------KQLEIQDFSVYWDTECEMLGKLPANQIQDAMTQCMLNREHQ-----YIFEPVCASALVRRNASKEPLRSRSTPRIEGLVQLEPMSLRLSQVQYLQIMTFLKELDRRERGMFYRKWRPKVPIHTNCRQWWMFAIEANL-NVIREQRRRCSWIFSLQRARDARTYTSLYFTRLRGIPLSPLEESELKRVEEE 379          

HSP 2 Score: 57.7658 bits (138), Expect = 3.468e-7
Identity = 134/526 (25.48%), Postives = 244/526 (46.39%), Query Frame = 3
Query: 8313 QIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQY-RTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLF-YEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKD-GIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTN---IEVFRIRPPRFI--AKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYV 9866
            QI   N+ + ++ + S   E + ++  +R    E EV  ++   EGL + L  K    PE+   + +F  GV + + RT  ++V + L +  IQ+DNQ+     PV+    P      L+    P +++++V + S       +K+  V  Q   +++++  +  +L F       Y E      + +M+  SL E  + +      K+  F+N  IS  +I LS   S+       AL          ++G  +   +D +  +  F + +       + + I  H+  + + Q   ++  +D LGNP G++  + +GV +L  Y  V G        +  V +GV +           + ++  GT+  G+   T+D  +Q + RE +       G  L  G  GL  G + G+T ++T  ++G K + G+ GFF G+GKGLVG VT+PV+G +DFAS + + +R +A  +   + V R+R PR     +D ++R Y   +AEG     ++T   D I +E ++
Sbjct: 3671 QISDFNQRRIIRSSASNEHEESAEDTKSRPHRQELEVTVNLE--EGLGISLVNK---SPEELVFATLF--GVDIHFTRTVTNEV-LELSVQNIQVDNQLLGSTQPVMLCVTPNSSDCNLNES-GPALQVNSVKVPSTLMLTELFKHLMVTAQRFTIIIEEKLLLKLLSFFG-----YGEAE---AELEMLDESLPERPSEEA--GPPKRYYFENLKISLPQIKLSVFTSQKLPPDLKALKG--------TLGFPLIRFEDAVINMYPFTRVHPYETQEIIFNDILKHFREELMSQAAQILGSVDFLGNPMGLLNDVTEGVSELIKYGNVGG-------LIRNVTHGVSN-----------SAAKFAGTLSDGLGK-TMDNRHQSE-RECIRYHGATSGEHLVAGIHGLAHGIIGGMTSIITSTVEGVKMEGGVSGFFSGLGKGLVGTVTKPVAGALDFASETAQTVRDMASLSSHRLGVQRVRKPRCCKGPQDLLLR-YSAVQAEGQEQLFRLT---DNIHSEFFI 4145          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Medaka
Match: vps13d (vacuolar protein sorting 13 homolog D [Source:NCBI gene;Acc:101163959])

HSP 1 Score: 160.999 bits (406), Expect = 1.833e-38
Identity = 115/393 (29.26%), Postives = 198/393 (50.38%), Query Frame = 3
Query:   27 VFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETT-NFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEE 1202
            + E LV  ++N YLG YV  L+  QL + LL G   LENL ++++A  +FDLP +V+ G I K+TL+IP+   +S+P +  +  L ++I P    +YD EKER    E K K L +  E+K K    +K      S    L  +IV+N+++ I ++H+R+ED S+     F+ G+ + N+S Q         ++++       K +++   +VYW++  +         ++  M   + + E +     ++ +P+  SA++  +   E   + + PR+   ++LE +    ++ QY  I  FL  +DR      +RK+RP VP+ +N   WW F     L   +R +RR  SW +        R YT ++  +L    LS  E+  +K  EEE
Sbjct:    1 MLEGLVAWVLNTYLGKYVSNLNTDQLSIALLKGAVELENLPLRKDALREFDLPYEVKAGFIGKITLQIPFYRPHSDPWVISMSQLNLIIGPAPPQEYDEEKEREDENERK-KHLLKALEDKFKCECEQKGESYWYSVTASLVTRIVENIELKIQDVHLRFEDDSSNLENPFSFGVCINNVSAQNPAKEMVQKLLRQ-------KQLEIQDFSVYWDTECEMLGKLPANQIQDAMTQCMLNREHQ-----YIFEPVCASALVRRNASKEPLRSRSTPRIEGLVQLEPMSLRLSQVQYLQIMTFLKELDRRERGMFYRKWRPKVPIHTNCRQWWMFAIEANL-NVIREQRRRCSWIFSLQRARDARTYTSLYFTRLRGIPLSPLEESELKRVEEE 379          

HSP 2 Score: 57.7658 bits (138), Expect = 3.270e-7
Identity = 134/525 (25.52%), Postives = 244/525 (46.48%), Query Frame = 3
Query: 8313 QIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQY-RTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKD-GIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTN---IEVFRIRPPRFI--AKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYV 9866
            QI   N+ + ++ + S   E + ++  +R    E EV  ++   EGL + L  K    PE+   + +F  GV + + RT  ++V + L +  IQ+DNQ+     PV+    P      L+    P +++++V + S       +K+  V  Q   +++++  +  +L F       Y E      + +M+  SL E  + +      K+  F+N  IS  +I LS   S+       AL          ++G  +   +D +  +  F + +       + + I  H+  + + Q   ++  +D LGNP G++  + +GV +L      G V G    +  V +GV +           + ++  GT+  G+   T+D  +Q + RE +       G  L  G  GL  G + G+T ++T  ++G K + G+ GFF G+GKGLVG VT+PV+G +DFAS + + +R +A  +   + V R+R PR     +D ++R Y   +AEG     ++T   D I +E ++
Sbjct: 3665 QISDFNQRRIIRSSASNEHEESAEDTKSRPHRQELEVTVNLE--EGLGISLVNK---SPEELVFATLF--GVDIHFTRTVTNEV-LELSVQNIQVDNQLLGSTQPVMLCVTPNSSDCNLNES-GPALQVNSVKVPSTLMLTELFKHLMVTAQRFTIIIEEKLLLKLLSFFG-----YGEAE---AELEMLDESLPERPSEEA--GPPKRYYFENLKISLPQIKLSVFTSQKLPPDLKALKG--------TLGFPLIRFEDAVINMYPFTRVHPYETQEIIFNDILKHFREELMSQAAQILGSVDFLGNPMGLLNDVTEGVSELIK---YGNVGG---LIRNVTHGVSN-----------SAAKFAGTLSDGLGK-TMDNRHQSE-RECIRYHGATSGEHLVAGIHGLAHGIIGGMTSIITSTVEGVKMEGGVSGFFSGLGKGLVGTVTKPVAGALDFASETAQTVRDMASLSSHRLGVQRVRKPRCCKGPQDLLLR-YSAVQAEGQEQLFRLT---DNIHSEFFI 4139          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Planmine SMEST
Match: SMESG000037244.1 (SMESG000037244.1)

HSP 1 Score: 6630.81 bits (17202), Expect = 0.000e+0
Identity = 3369/3397 (99.18%), Postives = 3372/3397 (99.26%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMK---------AKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKKSDSGYSLQSLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEIEADLIVLKAVVSKIIKIQKYFEDKKQYSNIS 10187
            MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMK         AKKMRKKLNQKTSSGGWFSGWFGKSDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIDEDDNEDEEFKDALEIMDNNVIIDKKKTSKLSKIKNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQ+TSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGD+NPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVKAQPPPIQPPKKAMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQ               IYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDD+GLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVLFSFLSKSIFSKKKETISQAIVKKSDSGYSLQSLDTVESPAEDLHD LDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNFPVVFAPFPPPRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFGHAVGGAAGAVSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIVGFVQGVTGVVTKPIDGAKKDGIEGFFKGVGKGLVGVVTRPVSGVVDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEIEADLIVLKAVVSKIIKIQKYFEDKKQYSNIS
Sbjct:    1 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKVSKLKANKKAKKMRKKLNQKTSSGGWFSGWFGKSDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIDEDDNEDEEFKDALEIMDNNVIIDKKKTSKLSKIKNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQNTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDSNPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVKAQPPPIQPPKKAMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQ---------------IYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDNGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVLFSFLSKSIFSKKKETISQAIVKKSDSGYSLQSLDTVESPAEDLHDQLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNFPVVFAPFPPPRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFGHAVGGAAGAVSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIVGFVQGVTGVVTKPIDGAKKDGIEGFFKGVGKGLVGVVTRPVSGVVDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEIEADLIVLKAVVSKIIKIQKYFEDKKQYSNIS 3382          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Planmine SMEST
Match: SMESG000037244.1 (SMESG000037244.1)

HSP 1 Score: 6518.72 bits (16911), Expect = 0.000e+0
Identity = 3325/3393 (98.00%), Postives = 3331/3393 (98.17%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKK----SDSGYSLQ-SLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEIEADLIVLKAVVSKIIKIQKYFEDKKQYSNIS 10187
            MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTSSGGWFSGWFGKSDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIDEDDNEDEEFKDALEIMDNNVIIDKKKTSKLSKIKNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQ+TSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGD+NPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVKAQPPPIQPPKKAMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDD+GLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVLFSFLSKSIFSKKKETISQAIVK     +DS +S + SLDTV S                                                     SGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNFPVVFAPFPPPRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFGHAVGGAAGAVSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIVGFVQGVTGVVTKPIDGAKKDGIEGFFKGVGKGLVGVVTRPVSGVVDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEIEADLIVLKAVVSKIIKIQKYFEDKKQYSNIS
Sbjct:    1 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTSSGGWFSGWFGKSDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIDEDDNEDEEFKDALEIMDNNVIIDKKKTSKLSKIKNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQNTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDSNPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVKAQPPPIQPPKKAMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDNGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVLFSFLSKSIFSKKKETISQAIVKAHLKINDSLWSDKFSLDTVGS-----------------------------------------------------SGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNFPVVFAPFPPPRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFGHAVGGAAGAVSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIVGFVQGVTGVVTKPIDGAKKDGIEGFFKGVGKGLVGVVTRPVSGVVDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEIEADLIVLKAVVSKIIKIQKYFEDKKQYSNIS 3340          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Planmine SMEST
Match: SMESG000037244.1 (SMESG000037244.1)

HSP 1 Score: 6508.32 bits (16884), Expect = 0.000e+0
Identity = 3325/3402 (97.74%), Postives = 3331/3402 (97.91%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMK---------AKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKK----SDSGYSLQ-SLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEIEADLIVLKAVVSKIIKIQKYFEDKKQYSNIS 10187
            MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMK         AKKMRKKLNQKTSSGGWFSGWFGKSDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIDEDDNEDEEFKDALEIMDNNVIIDKKKTSKLSKIKNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQ+TSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGD+NPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVKAQPPPIQPPKKAMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDD+GLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVLFSFLSKSIFSKKKETISQAIVK     +DS +S + SLDTV S                                                     SGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNFPVVFAPFPPPRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFGHAVGGAAGAVSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIVGFVQGVTGVVTKPIDGAKKDGIEGFFKGVGKGLVGVVTRPVSGVVDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEIEADLIVLKAVVSKIIKIQKYFEDKKQYSNIS
Sbjct:    1 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKVSKLKANKKAKKMRKKLNQKTSSGGWFSGWFGKSDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIDEDDNEDEEFKDALEIMDNNVIIDKKKTSKLSKIKNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQNTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDSNPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVKAQPPPIQPPKKAMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDNGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVLFSFLSKSIFSKKKETISQAIVKAHLKINDSLWSDKFSLDTVGS-----------------------------------------------------SGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNFPVVFAPFPPPRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFGHAVGGAAGAVSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIVGFVQGVTGVVTKPIDGAKKDGIEGFFKGVGKGLVGVVTRPVSGVVDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEIEADLIVLKAVVSKIIKIQKYFEDKKQYSNIS 3349          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Planmine SMEST
Match: SMESG000037244.1 (SMESG000037244.1)

HSP 1 Score: 6488.29 bits (16832), Expect = 0.000e+0
Identity = 3318/3402 (97.53%), Postives = 3324/3402 (97.71%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMK---------AKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKK----SDSGYSLQ-SLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEIEADLIVLKAVVSKIIKIQKYFEDKKQYSNIS 10187
            MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMK         AKKMRKKLNQKTSSGGWFSGWFGKSDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIDEDDNEDEEFKDALEIMDNNVIIDKKKTSKLSKIKNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQS       GKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQ+TSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGD+NPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVKAQPPPIQPPKKAMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDD+GLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVLFSFLSKSIFSKKKETISQAIVK     +DS +S + SLDTV S                                                     SGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNFPVVFAPFPPPRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFGHAVGGAAGAVSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIVGFVQGVTGVVTKPIDGAKKDGIEGFFKGVGKGLVGVVTRPVSGVVDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEIEADLIVLKAVVSKIIKIQKYFEDKKQYSNIS
Sbjct:    1 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKVSKLKANKKAKKMRKKLNQKTSSGGWFSGWFGKSDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIDEDDNEDEEFKDALEIMDNNVIIDKKKTSKLSKIKNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQS-------GKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQNTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDSNPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVKAQPPPIQPPKKAMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDNGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVLFSFLSKSIFSKKKETISQAIVKAHLKINDSLWSDKFSLDTVGS-----------------------------------------------------SGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNFPVVFAPFPPPRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFGHAVGGAAGAVSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIVGFVQGVTGVVTKPIDGAKKDGIEGFFKGVGKGLVGVVTRPVSGVVDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEIEADLIVLKAVVSKIIKIQKYFEDKKQYSNIS 3342          
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Planmine SMEST
Match: SMESG000037244.1 (SMESG000037244.1)

HSP 1 Score: 6480.97 bits (16813), Expect = 0.000e+0
Identity = 3310/3393 (97.55%), Postives = 3316/3393 (97.73%), Query Frame = 3
Query:   24 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTXXXXXXXXXXXXXDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIXXXXXXXXXFKDALEIMDNNVXXXXXXXXXXXXXXNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVXXXXXXXXXXXXXMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVXXXXXXXXXXXXXXETISQAIVKK----SDSGYSLQ-SLDTVESPAEDLHDHLDQTSTASINNVNNKNQKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNXXXXXXXXXXXRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFXXXXXXXXXXXSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIXXXXXXXXXXXXKPIDGAKKDGIEXXXXXXXXXXXXXXXXXXXXXXDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEIEADLIVLKAVVSKIIKIQKYFEDKKQYSNIS 10187
            MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTSSGGWFSGWFGKSDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIDEDDNEDEEFKDALEIMDNNVIIDKKKTSKLSKIKNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQ+TSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGD+NPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVKAQPPPIQPPKKAMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQ               IYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDD+GLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVLFSFLSKSIFSKKKETISQAIVK     +DS +S + SLDTV S                                                     SGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNFPVVFAPFPPPRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFGHAVGGAAGAVSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIVGFVQGVTGVVTKPIDGAKKDGIEGFFKGVGKGLVGVVTRPVSGVVDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEIEADLIVLKAVVSKIIKIQKYFEDKKQYSNIS
Sbjct:    1 MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDDFDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDPEKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNLQISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDSTREFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVSFLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDITGFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRMWSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNITLCRQQAEMKAKKMRKKLNQKTSSGGWFSGWFGKSDTETSSSLASDGSDELQKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFIISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPDGSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSDTVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTDIKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLIFGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNHILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELILHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLPDSETASLTSTIDEDDNEDEEFKDALEIMDNNVIIDKKKTSKLSKIKNMIDLDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDMAVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKTSPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEEEKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLATEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVIVANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKKIIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFLYNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKLDINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQNTSVVLEESKITLSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIAARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILPKDDKSGSARTASDESITVQFGFKMEFLDVELFKGDSNPNEWASEMPPDKCLTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNIKNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVKAQPPPIQPPKKAMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEILLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSESRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISKIMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGLMEKEKINTRNDGVESGFDVAQILLDVEQRRADQ---------------IYLRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVSYFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDNGLFDEEDFEDLISIEPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFGAPYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDEKQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRGNVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDKSETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGALWNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQIVNSSETLRTYKLDINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTYHYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQANVTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVLFSFLSKSIFSKKKETISQAIVKAHLKINDSLWSDKFSLDTVGS-----------------------------------------------------SGKVMCKKKPVTYEVGVKINLSSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPRSEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQITEEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVMYAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWDSNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQELAYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEVEEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHLRIYKIQIDNQMSDCNFPVVFAPFPPPRSVALDTGLKPLIELSAVIIKSGETGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQMIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTALHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHYVSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEFVEGVAYGVKSLFGHAVGGAAGAVSRIVGTMGKGIAALTLDADYQRKRREQLAKRPENLGVGLAQGGKGLIVGFVQGVTGVVTKPIDGAKKDGIEGFFKGVGKGLVGVVTRPVSGVVDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGIIRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRIFHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSKDNITKEIEADLIVLKAVVSKIIKIQKYFEDKKQYSNIS 3325          
The following BLAST results are available for this feature:
BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
VPS13A0.000e+029.83vacuolar protein sorting 13 homolog A [Source:HGNC... [more]
VPS13A0.000e+030.14vacuolar protein sorting 13 homolog A [Source:HGNC... [more]
VPS13A0.000e+030.11vacuolar protein sorting 13 homolog A [Source:HGNC... [more]
VPS13A2.978e-15628.84vacuolar protein sorting 13 homolog A [Source:HGNC... [more]
VPS13C9.921e-14630.40vacuolar protein sorting 13 homolog C [Source:HGNC... [more]
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BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
T08G11.14.805e-13127.07pep chromosome:WBcel235:I:8905644:8916805:-1 gene:... [more]
T08G11.16.518e-13738.51pep chromosome:WBcel235:I:8905644:8916805:-1 gene:... [more]
C25H3.114.013e-4129.16pep chromosome:WBcel235:II:5669788:5689030:-1 gene... [more]
C25H3.114.257e-4129.16pep chromosome:WBcel235:II:5669788:5689030:-1 gene... [more]
C25H3.114.289e-4130.13pep chromosome:WBcel235:II:5669788:5689030:-1 gene... [more]
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BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Vps131.283e-14838.92gene:FBgn0033194 transcript:FBtr0310024[more]
Vps131.283e-14838.92gene:FBgn0033194 transcript:FBtr0088910[more]
Vps13D2.278e-2926.51gene:FBgn0052113 transcript:FBtr0273390[more]
Vps13B3.270e-1130.71gene:FBgn0039727 transcript:FBtr0085505[more]
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BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
vps13c1.781e-17229.96vacuolar protein sorting 13 homolog C [Source:ZFIN... [more]
vps13c9.382e-16729.35vacuolar protein sorting 13 homolog C [Source:ZFIN... [more]
vps13c1.173e-14839.26vacuolar protein sorting 13 homolog C [Source:ZFIN... [more]
vps13a1.798e-13939.12vacuolar protein sorting 13 homolog A [Source:ZFIN... [more]
vps13d1.285e-4628.81vacuolar protein sorting 13 homolog D [Source:ZFIN... [more]
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BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
git21.837e-15739.72G protein-coupled receptor kinase interacting ArfG... [more]
git26.979e-15639.25G protein-coupled receptor kinase interacting ArfG... [more]
VPS13A2.995e-15540.60vacuolar protein sorting 13 homolog A [Source:NCBI... [more]
VPS13A3.167e-15540.60vacuolar protein sorting 13 homolog A [Source:NCBI... [more]
git26.583e-15439.53G protein-coupled receptor kinase interacting ArfG... [more]
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BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Vps13a6.834e-15328.25vacuolar protein sorting 13A [Source:MGI Symbol;Ac... [more]
Vps13a5.952e-2128.20vacuolar protein sorting 13A [Source:MGI Symbol;Ac... [more]
Vps13c1.296e-15828.89vacuolar protein sorting 13C [Source:MGI Symbol;Ac... [more]
Vps13a2.902e-15439.35vacuolar protein sorting 13A [Source:MGI Symbol;Ac... [more]
Vps13c1.221e-14042.23vacuolar protein sorting 13C [Source:MGI Symbol;Ac... [more]
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BLAST of Vacuolar protein sorting-associated protein 13A/C vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q96RL7|VP13A_HUMAN0.000e+029.83Vacuolar protein sorting-associated protein 13A OS... [more]
sp|Q5H8C4|VP13A_MOUSE4.780e-15228.25Vacuolar protein sorting-associated protein 13A OS... [more]
sp|Q8BX70|VP13C_MOUSE9.066e-15828.89Vacuolar protein sorting-associated protein 13C OS... [more]
sp|Q709C8|VP13C_HUMAN5.817e-18029.49Vacuolar protein sorting-associated protein 13C OS... [more]
sp|A1Z713|VPS13_DROME1.286e-14738.92Vacuolar protein sorting-associated protein 13 OS=... [more]
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BLAST of Vacuolar protein sorting-associated protein 13A/C vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5K4EAP50.000e+038.47VPS13C protein, putative OS=Schistosoma mansoni OX... [more]
W6V2890.000e+034.52Vacuolar protein sorting-associated protein OS=Ech... [more]
A0A068Y6L90.000e+034.04Vacuolar protein sorting associated protein 13A OS... [more]
A0A068WCF90.000e+034.13Vacuolar protein sorting associated protein 13A OS... [more]
V4C0Y20.000e+034.16Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
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BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
vps13a1.096e-16629.58vacuolar protein sorting 13 homolog A [Source:ZFIN... [more]
vps13a1.225e-16634.47vacuolar protein sorting 13 homolog A [Source:ZFIN... [more]
vps13c5.113e-14740.49vacuolar protein sorting 13 homolog C [Source:ZFIN... [more]
vps13c5.460e-14740.38vacuolar protein sorting 13 homolog C [Source:ZFIN... [more]
vps13c1.398e-14242.21vacuolar protein sorting 13 homolog C [Source:ZFIN... [more]
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BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000003418.11.257e-2958.24pep scaffold:Pmarinus_7.0:GL489749:646:4444:-1 gen... [more]
ENSPMAT00000003332.14.859e-2858.45pep scaffold:Pmarinus_7.0:GL487813:5059:8070:-1 ge... [more]
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BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
VPS132.334e-4723.14Protein involved in prospore membrane morphogenesi... [more]
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BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO345057.856e-15237.97Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO319071.487e-7532.56Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO319063.484e-4834.95Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO420774.604e-4650.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO414504.920e-4328.67Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
vps13a5.978e-16234.22vacuolar protein sorting 13 homolog A [Source:NCBI... [more]
ENSORLT00000000640.31.396e-12238.85pep primary_assembly:ASM223467v1:3:5428377:5447491... [more]
ENSORLT00000031398.15.395e-11333.29pep primary_assembly:ASM223467v1:3:5374961:5398684... [more]
vps13d1.563e-3829.26vacuolar protein sorting 13 homolog D [Source:NCBI... [more]
vps13d1.833e-3829.26vacuolar protein sorting 13 homolog D [Source:NCBI... [more]
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BLAST of Vacuolar protein sorting-associated protein 13A/C vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000037244.10.000e+099.18SMESG000037244.1[more]
SMESG000037244.10.000e+098.00SMESG000037244.1[more]
SMESG000037244.10.000e+097.74SMESG000037244.1[more]
SMESG000037244.10.000e+097.53SMESG000037244.1[more]
SMESG000037244.10.000e+097.55SMESG000037244.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001501 ID=SMED30001501|Name=Vacuolar protein sorting-associated protein 13A/C|organism=Schmidtea mediterranea sexual|type=transcript|length=11021bp
TAATTAGATGAAATAACTATCAAATGGTTTTTGAATCGTTAGTCGTAGAG
TTAATAAACAAATATCTAGGAGATTATGTTGTAAAACTTGATAAATCTCA
ATTGAAATTGGGTCTTTTAGGAGGTAATGCAACGCTGGAAAACCTTGATA
TAAAAGAAAATGCTTTTGACGATTTTGATCTTCCTGTTAAGGTTCGACAA
GGACATATAAGTAAACTCACGTTAAAGATTCCCTGGAAGCATCTGTATTC
AGAGCCGGTTATTGCAGAACTGGACGGATTATATGTTCTAATAATACCAA
ATTCTGAAATGAAATATGATCCTGAAAAAGAACGATCTTATCTTTTTGAA
ACCAAACAGAAGGAATTAAATCGCATTGAAGAAAATAAACTGAAAAGAAA
TGACCCAAAGAAAGAAACAGAAACCAAAGACTCGTTTGGTGAAAAATTAG
CGGCACAAATAGTTAAAAATCTACAAATCAGCATTAGTAATATACACATC
AGATATGAGGACACGTCCACCTGTCCAAACAGACATTTTGCCGTTGGAAT
CACTTTGGCAAATCTATCGTTCCAGACATGTGACTCAAGTGGAACTAGTA
TAATTATCAAAGAAGATTCAACTAGAGAGTTTTTCAAATCTGTCAAAATG
GATTCATTAGCTGTTTATTGGAACAGTAATCCGAAACCTTTTTCCAGTGA
AAACAGAGCAACTTTAAAAGCAATTATGAAAAATAATATAAGTTCGTCGG
AACGCAAATTAACAGATGTCAGTTTTTTAATTCAACCTATCTCCTGTTCC
GCGATGATGCACGTTCACATGAAACCAGAAACGACAAATTTCACAGTGCC
CAGAATGTCATTAGATTTGAAATTAGAACAAATCGGATTCGCGTTTAATC
GCAATCAGTATTGCGATATTACGGGATTCCTTGATTCGATGGATCGGGCA
TGGACGAAAAATCGGTTTAGAAAATATCGACCAAACGTTCCCATGAGATC
AAACATCAGCATTTGGTGGCATTTTCCATATAACGTGATTCTCGAAGAGC
AAGTACGAAGAAGAAGACGTATGTGGTCATGGAAATACATTCAAGCCCAT
CGCAATCGCTGTCGTCTTTACACTGATATCTGGGAACAGAAACTATTGGA
TAAGAAATTGTCTCAATCTGAACAAGATACTATTAAAGTTTGTGAAGAGG
AATTGGATTTACTTAACATAACATTATGTCGTCAGCAAGCAGAGATGAAG
GCGAAAAAGATGAGGAAAAAGTTAAATCAGAAAACTTCAAGTGGAGGTTG
GTTTTCCGGATGGTTTGGCAAATCCGATACAGAGACATCATCATCCCTGG
CATCAGATGGCAGTGATGAATTGCAAAAGAAAGTGCGTAGTGAATTCACA
CAAGAAGAAAAAGCTAAACTCTATCAAGCCATTGGTTATTCGGAACTGAC
TACAGATCCTGATATTCCGATAGAATACGTTTCCTTGAAATTGAATTTAG
AATTGCAAAAGTTGAAGTTCATAATCTCGGACAATTCCTTGAAAGAATAT
AAATTAATGAAATTGGTCATACATTCCATGTCTGCCCATGTACAACAACG
CAGCTCGGCTGATGCAATCAGTGTGAATCTAATTATGAATAATATGCAAG
TCACCGGCACCAAGGGTCCCGATGGCTCCGTGCCCACCCTCGTCAGCACT
CAAGCTCAATCAAAGCTGATGAATTTACTAATTGTTGATTTTGAAACGAA
TCCTCTCGATAAATCTGCAAATCAAAAGATTAATATTCGAGCTGAACCAC
TAGAAATTATTTATGATTCTGATACAGTTAATCACATTGTCAAATTCTTG
ACTCCACCAGAAGAACTGCAACTATACGAGATATCTACCCAAGTATTAGC
ATCTATTGAGGAGGTGAAGGAAATGACTGCCACTGGGCTACGACATTTGG
CTGATCGGAGAATTTATACTGATATTAAAGTCGACATAAAACCAAGTTAT
TTCATTTTACCCGAGCGTGGGAAATATACAAAAGATTGTAAAATGCTTCT
TGTTGATTTGGGCAGTATTTTGATTGTTTCTGAAAGAACAAGCATGCCAG
GTTCCGGTGATACTGATCTTATTTTTGGAAAAGATGGCAAACTCAATTAT
GACGTGATGAAAGAAACTGCCTATGATAAATTTCACATTATTTTATCAAC
CATACAAGCTCTCATGATCGAACAAGGGGACGATTGGCGCAAGTTGCGAT
CCCAGGAGTCATCCAAAAATCATATTTTAAGACCTTTTGGTTTTGAGTTT
AATTTAAAAAAATGTGTCATTACCAACGATGCAAATCTTCCAAAGATATT
TGTCAAAGGTTGTCTTCCGCATTTGGGAGTTATTATATCAGATAGAGTTT
TACAATCGGTTCTTGAGCTAATTCTGCATATCCCTTTTCCTCAAACCGCT
CCTCCGAAACCAGTTCCAACTACAGAAAGTATTTTAAGGGAATACAGTGG
GAAACAGCTTGGCATTGCACCTAAATTAAATTTAACAAGTAACCAAGTGT
TGAGGGCTATTAACGTACTTCCTGATTCGGAAACCGCCAGTTTAACTAGC
ACCATTGACGAGGACGATAATGAAGATGAAGAGTTCAAGGATGCTCTGGA
AATCATGGATAACAATGTAATCATAGACAAAAAGAAAACCAGCAAACTAT
CCAAAATAAAAAATATGATTGATTTGGACGCACTATTTGTGATAAATACG
TTTGAGCTGCAAGTTCTTCAAGATATTAAAAATACACTTGTGGAAAAGAA
TGGTGCAAAAGAAATCGTGAAATTAATTGTCCATGAAATTGGAACAACAT
TGACATTGAAGCGATTTGACATGGCGGTCCAAGCCTACATCGGGTCACTA
ATTTTGGCTGTTCCACAATTTGTTGATCCAAGTACAAAATCACCGGTTTG
TTTGGCACAAACGAAAAAACATACCAAAGGACAAAATCTGTTGTTTATTA
ATTTGTTAATTGTTGAGAAAACCTCCCCCGATTTTGTAACAGTTCATGCC
AATACCCGACACAAATTAGAATGCCAGTTCAAATCCATTGAGGTTTGTCT
TCACCAAGAAGCTCTTTTAGTTGTGCTAAATTTCATAAACTCATTGATAC
CAGTTTTGAACATCGTTGAAGAAGAAAAAACTGAAACCTGGTCTTCGCCA
AATAAAGAGGAGACACGCAAACAGACAACTCTTGAAAAAGAAAATGAGGC
CATTGAAGACCTAAAAAGGCAGAAGGCTGATACATCTATAATGGGTTTGA
TTACTGATCGAATAGATTTAGCGACGGAAAAACGAACCGTTCGATTGAAA
GCCGAGGCCGTTTCAGAGCAGTCAGATTACAACGGCTACGATTCAGGGAA
AAAGATTTTCATCCCAGAGATTACTATGACCCAATGGGCAATAACTGCCA
GCCTTCAAAAGTTGCGAGTCATTGTTGCTAATGAAAAAGACAATAATATT
CTTGACATTTCAATTCATGGTATGAGAGCAGATGTGAAAACGAGTTATTA
CACAACTGAAGTCAGCATAACTATCACAGATATGAGAATTCAAGATCCAA
GTCCTTTGGCTGTCTATAAAAAAATAATCTGGATTGAGCGCGATGAAAGC
ATATTTTCCATGCAAGTAGCCTTATTTAATCAAGGCACATCAACTCCGGG
CTATGAATTAGATTTGTCAAGGGTCGATCATATGGTCAACATAGAAGTCG
GGAAAATTAAAATCATTTTTCTATACAATTTCGTTTTGAGAATTCAAACT
TTTCTTAACAGTTTCCAAGAGTCCTTACAAACGGTTGCTAACAAAGCTCA
AGAGTATTCTGAAGCTGCAGCCAATAAAATTACCAATGTTCTAGAGGAAC
CTCCTTCTAGTCGAACGAAACTTGACATTAATGTTAAGGCACCTGTGATT
TATATTCCAGAAAATTCCAAGTCTGTAAATGCTTTGATGGTCGATCTAGG
AGCCTTAACTTTCAAAAATGATTTTGCCATTGATCAGAGCACATCTGTCG
TACTTGAAGAATCCAAAATTACACTTAGTAACCTACGAGTATCTCGATCC
ATTTTATTGGAAAATACAGTAAAAAACGAAAAGGGTATTTTGCATCCAAT
TAATATATTCATGGATATGACTCGCAATCTGTCATGGAATCAAGATCATA
CAATACCGCAGATCAGCATTGCTGCCAGAATGACATCTATCAGTGTTGGA
ATGTCGCAAGGAGATTATAATATGTTTCAACTAGTTCTTCAATCCAACTT
CAATGAGGCCATCAACCAAGTTCCTAGTGTTCCTGAATCAAAAGAAGAAG
TCAAATCAAGGAAAATTTTACCAAAAGATGATAAATCTGGATCGGCTCGC
ACTGCTAGTGATGAATCGATAACGGTTCAGTTTGGATTCAAAATGGAATT
CTTAGATGTTGAACTGTTTAAAGGAGATGCGAATCCAAATGAGTGGGCCA
GTGAAATGCCTCCCGATAAATGTCTGACGAAGATGAAATGTGAAAACTTG
TCAGTTGAAGGTTCAATGCACCTCAATTCAGCAATGGAAGTTAGTGTCCG
GTTAAGTAATATTAACGTTTTGGATACGCAACTCGATTCGGCTAAAAAAA
TTAATGAATTAATTAGAAATATAAAAAATGACTCCAGTGATTTTATTTCA
ATCAAATTTATTCAAGATAAGAATTCTAATAGTAAACTGATTGTCGACAT
TGCGGGAATATATATGTGCATCTGCGTCGATTATTTGATGATGTTGACAG
ATTTCTTTGTGAAAGCTCAACCTCCGCCAATACAGCCCCCGAAGAAAGCC
ATGGTTCCTGTTAGAGGATATGAGAAAAATCCTCGTAAATATAGAAAAAA
ACCACTTGCGAGTCCAGTTGAACCTCGGCTGGCTGATTTTGAAATAGATG
TCAAAGTTGCACGACCTGAAATCTTACTCGTAGAAGATATGCTCAATCCT
GATTCAAATGCAATAATGGTTCAGACAAATTTTGTTCTTAAATATTCAAT
AATGAAAGAAAGCGACATTATTAATGTTGATATCAGTGATTTCCAGGCCG
TTTTCTGTCCGTTTCGATCAGAAAGTCGTAAGAAAAATACTATGGATATA
ATTTCTCCTACAAGCATTTCTCTATATTGCAACAAACCGATAAATAACGG
AATGCAGGGCAGCCTCACCATGGAACACCTAATCATTAATATTACTCCGG
CTGTTATCCAAAGCATCTCGAAGATCATGAACACCCTTACTGCTCAATCT
ACCGATGAGACATCTACTAGAGATGGATCATTTATAAATAATCAAGATCA
ATTGATTGTCGACAATTTCTGGGAGAAACGAGAATTCGATGAAAATGAAT
TTTTATTTATTTCTAAAGGATTGATGGAGAAAGAGAAAATTAATACCCGT
AATGATGGAGTGGAAAGTGGATTTGATGTGGCACAAATTTTGTTGGATGT
TGAGCAACGAAGAGCCGATCAAAAATTCGACAATGGTGATAATTTAGATG
AACATAAAGAGTCTAAAATTTATCTTCGAATGCCTGATATCAAAGTAATT
TTGGAATCTGAAGTTGCAAAGAAATGTGTACCGATGCTGATTTTTGAATG
TTCAATGGATGGAGATTTAAAAAATCCTTTAACTCAAATTTGTCTCGGTG
TTACTGTTTCGGTTTCAGTTAGCTATTTTAATGACCACATTAACGTTTGG
GAACCCTTATTAGAGGTGTATCCAGAAGAGCATAATCGGCAATATTCATT
TGAAATCGAATACATTTCTTGTGATGATAGTGGATTATTTGATGAAGAAG
ACTTCGAAGATCTTATAAGTATTGAACCGTCTGCTATCTCAATTTTACTT
TTATCCCGTGACGCCCTTGAATTAACAGTCAGTAAAAGCGCATTAGATGT
ATTAAGCAATTTAGGAAAGGCTTTTGAAGATGCTTATTCACTTCATTTGG
AACAGCATAAATCTATTTTCGGAGCCCCGTATGTTGTAAAAAATACTTGT
GGAAAAGATGTTGTATTTTATATTAACAAAGAATTTGATTTAAAGCCTTG
CAACTCTGATGAAGAATTTAAAGTTTACTTAAAAGCTGATGGCGAAGTTC
AATTATACGATGCGGTCGATGAAAAGCAATCCTTAATTACCGAAGAAAAA
CAAGCTAAAATTGTTAAATTTGGTTTTGCCGATTCCATTAGTCGAGAATA
TTTGTCAATTTATAGAACTGAAGTGCGAGCTTTGTTATTCAGCCCCAAGG
ATAATGAAGGTCCCAGTCGCGGAAATGTCAGTGAAAGAGAGATTAATACG
TTAATTGTATCGACTACCGCATATCTGGGTATGAAGCAACTTGAACTTCG
ATCAGTTTTACAAGTCTTCAATAATACTGATGATGTTATAACTTTATATT
ATCTTCGGAAGAAGAGTGACAAAAGTGAAACTGCTAAAAATTATCTCCTA
CTCGAAGAAGTATTAGGAGGGAAATGTTACAATGTTCCTATACCAACACT
TTTGACCAGTAATGGTAAAATTTACATTAGTGTGAAACAATTGAATACTG
AAATATCTAACTCTGGAGCGTTGTGGAATATAGGAAAAAATGCCCAGCCG
TCCGACACTCAAATTTTGTTATGTAAAAGCAATACGGGTGTTATGTATTA
CTATTCAGTGGCTTTGAGCATTGAAGAAGTTCAAATTGTCAATTCCTCTG
AAACCTTACGCACTTATAAACTTGATATTAACGTTCCATTGGCATTACAC
AATCAGCTGCCGATTAAAATCAGTTATCAATCACAGATTTTCAGTCATGA
CATTAAACCCGGATGCCTTGAGCTTGTTAGAACGGCTTGTCTAAAAGTAC
AGTCAAATATCAAAGTCACATACCACTATCAAAAGAAAGAATGGTTTGGT
TCTTTGGATCTAGGGCCCAAAATGGAAGAACTCAGTGTTATAAATTTTAA
AGCTAAAGAAGGCTACTCTGAGTCATTTATGGATCTCGGTGTACATTTGG
CCATCAAAAATGGTCAGGCTAATGTGACAATCTACTCACCTTATTGGATG
TTAAATAAAACCGGTTATACATTGACGTATAAGGCAGGAGATGATTGCCA
GGTTGAACACGATGCCGCTAATACCGATGCGGTTCTCTTTTCGTTTCTTT
CCAAGTCGATTTTCTCAAAAAAGAAAGAAACCATCTCACAAGCCATCGTA
AAGAAATCAGATTCAGGGTACAGTTTACAGTCTCTTGATACCGTTGAGAG
TCCTGCTGAGGATTTGCATGATCATTTAGATCAAACGTCAACTGCCTCAA
TAAATAATGTTAATAATAAAAATCAGAAATTTGCAAAAACTGTGAAGGCT
CATCTTAAAATTAATGATTCGTTATGGTCAGACAAATTCTCTCTTGATAC
TGTTGGTAGTTCGGGCAAAGTCATGTGTAAAAAGAAACCAGTAACCTATG
AAGTTGGGGTAAAGATTAATTTATCTAGCTCGGGTTTAACGAAAATTGTG
ACATTTTTACCTTATTATATGATTTTAAATCAGTCTTCGATTGTCCTTTA
TTGCAATGAATTGGTGGAAAATGCGGGCTGGATAAAGGTGCCTCCAGGAG
AAGCAGTTCCATTTTGGCCACGATCTGAAGTCGGGAAGTCAATGATGATG
CGTTGCAAAGTTGAAGACCGAGAAGTCTTAGTAACTCAGCCGTTTCCATT
TTATGAATCACATTCAATTTTAATGAAATTGGAGAAAAAGTTTGTCGGAA
TATTTGTTGAAGTTCAAATTACTGAAGAAGCAAATTTAATAAATCTTCAA
CTTTATCAAGAAGGAATGGCATTAGTTAAGCTGATCAATCATTTAGGGGA
TGACGTTGAAATACACTTCAAACAATATAATACACCTCAGGAATTAATTT
TGCCAGCATATCAAACGGTAATGTATGCTTGGCAAGATGGACGCCAGAAG
CGAGAATTTATTTGGCGTGTTGAAGGTGACAAGAAGCAACATTCCAATAA
TCTGACTTCCGACGGGATAGAAGATTTTTACATTAATGAAATGAAAGCTT
ATTGGGTTTCATTTGTCTGGGATTCCAATCGAATATTACTGTTTACTAAC
GATATTCACGTGGCTAAGCATGCAAGATTGAGTGCTGATTTGGAGAGCAT
CGACCAGGAGATTACTATTTCTCTACACTCAATAGGTTTGAGCCTTGTCG
ACAATTCGTTGCAGCAGGAACTGGCATATATTTCTATCACCAGCTCGGGA
GTTAAATGGAATCAAATTCGCCGTAAAAACCGACACAAACCGCTTAAATT
GGCTCAAAGTGAAGCTTTGGAAGCAGCTTTTGATGAATTTGTGAACAGGC
TTAAAACGAGTGAAGCAGAAGTTGAAGAACATATTGTCAAAGTAGAAGGT
TTGGAAGTCGATTTGAGTAAGAAACTCATGATAAAACCGGAGAAGTGTGA
ATTGAGTCGAGTTTTCGAACCGGGTGTATTTTTACAATATCGCACGTCTC
CACATCAAGTTCATGTTCATTTAAGAATTTACAAAATTCAGATTGATAAT
CAAATGAGTGATTGCAACTTCCCTGTGGTGTTTGCTCCTTTCCCCCCACC
GAGATCTGTTGCTCTTGATACTGGCTTGAAGCCTCTTATTGAGTTGTCTG
CTGTGATAATTAAATCGGGAGAAACTGGTGCTATGAGATACAAATACTTC
CGAGTTCTTATTCAAGAGATGCAATTAATGTTGGATCAAGGTTTCGTAAA
TTCAATTCTCGACTTTGTTTCTGGAAGTGTCCCGACATATGACGAGGAAG
CAATGTTTGTCAAAGATTGTCAGATGATCCGCGCTAGTCTCATAGAGACA
TCAACAATCAAAACTATTCTTTCCGGTGGTAAAAAATGTGTTTTCGACAA
CTTTCATATTTCTCCAATTAAGATCCATTTGAGCTTTTCTTTATCGCGTG
TTAATCGGACAGGAAAAACTGCTTTACACAGTGAAGTTCTCAATTTGTTT
CTGCAATCAGTTGGAATAGCAATCACTGATATGCAAGATGTTATATTTAA
ATTGGGATATTTTGAAAAGCAAAATAGTATTATGCCTATGAATCAAATGG
TTTCATCTATAACTAGTCATTATGTTAGTCAAGCGCTTCGTCAAATGTAT
GTCTTAGTTTTGGGTTTGGATGTACTTGGCAACCCATTCGGAGTCATTAG
AGGCATGGCTCAGGGTGTTGAAGATTTATTCTATGAGCCTGTCAGGGGAG
CAGTTTTGGGCCCAGAGGAATTTGTTGAGGGAGTAGCATACGGTGTTAAA
AGTTTGTTTGGGCATGCAGTTGGTGGAGCAGCTGGTGCTGTGTCGCGGAT
TGTTGGAACAATGGGTAAAGGTATAGCTGCATTGACATTGGATGCCGACT
ACCAACGTAAACGTCGAGAACAACTTGCTAAACGACCGGAAAATCTTGGC
GTTGGATTAGCACAAGGTGGCAAAGGTCTCATTGTTGGATTCGTTCAAGG
CGTAACTGGAGTTGTGACTAAACCGATTGACGGGGCGAAAAAAGATGGAA
TCGAAGGATTCTTTAAAGGTGTAGGTAAAGGCTTGGTTGGGGTAGTTACT
CGCCCGGTTTCGGGAGTAGTAGATTTTGCCAGTAGTTCTTTTGAAGGAAT
CAGAAGGGTTGCCGACACAAATATTGAGGTATTCCGAATCAGACCTCCTC
GATTTATTGCAAAAGATGGTATTATTCGTTCGTATGGACTCCGAGAAGCC
GAAGGAAACAATATTTGGATTAAAATAACAGAGAAATGTGATCCTATACC
AAACGAATTGTATGTTTTCCATGTTTACTGCAAATCCAAGAAAAATGTTA
TTTTCTTAACTAACCAGCGGATTTTCCACTTAGATCGCAATGAAATCCTC
GGTATTTGGTCTATTCAGTGGCAAATAGCCTATGAGAAGTTGGATGGACC
GCCCACTATCAATGAGTCTAAACATACTATTGTTTTTACTTTGAAAGAAA
AACAGAGAAAATTATTTTCTAAAGACAATATTACCAAAGAAATCGAAGCT
GATTTAATAGTTTTGAAGGCCGTTGTGAGCAAAATAATTAAAATTCAAAA
GTATTTTGAAGACAAAAAGCAATATTCGAACATATCTTGATCTTGCAAGT
ATTATTTGTCATTTATACTGTTGATTGTCGAATTTTGCCTTATGATGTGA
ACTGTTATTATATCTGTCATTATAAAAATGACTATGTTTATCTTTTTATT
TGTGATTTTCGGATAGATGGCATCCATTTCAATAACAAATTTTTATATAG
AATATTCTGTGAATGGATTGAAATAAAATAAATAAATAATGATAGGGCTT
TGTTAGCTTGGGATTTTTACTCATTTTTGTCGCGCTGTGTTGGATAGGAT
TTTTATCGCATTGGCTTACAAATTAATATATTTTATTTCTGAAAATGTGA
TTTTTTAAAATTAAAAAAGATTTTCTGAAATATGAATATTACTAAATCTG
TGCGACAAACGTCAAAGGGTTCATTTGGGTCACTAGAGGCGATTTATCAA
TAAATTTCTCCTTTTAAACTTATTGATTTAAAAAACGAAATATTAAAAAA
AAAATTTGGTAAAACGTTTGATTTGGTTCAAAATTATTCGAAAGTAATGT
TTTTTCTCCTTATGATAATTAATTAATAGATTTGTTAATATTCCTAACTA
ATTTTGACAAAAGGTAGAACAGAACACTTTTTAATGAAATCTCCAACACT
GTTCACTGCTGGTTGAGTGAGAGTTATTTCTATTTGTCCAGGTGACAATT
CATAAATAAATTTCTGTTTTGGAATTTATTGGTTCCCATATATAAAGACA
TTTTTAAATTTTCGTTGAATTCTTTAATTTGATTAACTATTGAATGAGAA
TAATTTTTTTATGTAACAACTTATCAATAAATTTGTGTAGATCTTTTATT
TTTTAAATATTATTACTGTAG
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protein sequence of SMED30001501-orf-1

>SMED30001501-orf-1 ID=SMED30001501-orf-1|Name=SMED30001501-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=3389bp
MVFESLVVELINKYLGDYVVKLDKSQLKLGLLGGNATLENLDIKENAFDD
FDLPVKVRQGHISKLTLKIPWKHLYSEPVIAELDGLYVLIIPNSEMKYDP
EKERSYLFETKQKELNRIEENKLKRNDPKKETETKDSFGEKLAAQIVKNL
QISISNIHIRYEDTSTCPNRHFAVGITLANLSFQTCDSSGTSIIIKEDST
REFFKSVKMDSLAVYWNSNPKPFSSENRATLKAIMKNNISSSERKLTDVS
FLIQPISCSAMMHVHMKPETTNFTVPRMSLDLKLEQIGFAFNRNQYCDIT
GFLDSMDRAWTKNRFRKYRPNVPMRSNISIWWHFPYNVILEEQVRRRRRM
WSWKYIQAHRNRCRLYTDIWEQKLLDKKLSQSEQDTIKVCEEELDLLNIT
LCRQQAEMKAKKMRKKLNQKTSSGGWFSGWFGKSDTETSSSLASDGSDEL
QKKVRSEFTQEEKAKLYQAIGYSELTTDPDIPIEYVSLKLNLELQKLKFI
ISDNSLKEYKLMKLVIHSMSAHVQQRSSADAISVNLIMNNMQVTGTKGPD
GSVPTLVSTQAQSKLMNLLIVDFETNPLDKSANQKINIRAEPLEIIYDSD
TVNHIVKFLTPPEELQLYEISTQVLASIEEVKEMTATGLRHLADRRIYTD
IKVDIKPSYFILPERGKYTKDCKMLLVDLGSILIVSERTSMPGSGDTDLI
FGKDGKLNYDVMKETAYDKFHIILSTIQALMIEQGDDWRKLRSQESSKNH
ILRPFGFEFNLKKCVITNDANLPKIFVKGCLPHLGVIISDRVLQSVLELI
LHIPFPQTAPPKPVPTTESILREYSGKQLGIAPKLNLTSNQVLRAINVLP
DSETASLTSTIDEDDNEDEEFKDALEIMDNNVIIDKKKTSKLSKIKNMID
LDALFVINTFELQVLQDIKNTLVEKNGAKEIVKLIVHEIGTTLTLKRFDM
AVQAYIGSLILAVPQFVDPSTKSPVCLAQTKKHTKGQNLLFINLLIVEKT
SPDFVTVHANTRHKLECQFKSIEVCLHQEALLVVLNFINSLIPVLNIVEE
EKTETWSSPNKEETRKQTTLEKENEAIEDLKRQKADTSIMGLITDRIDLA
TEKRTVRLKAEAVSEQSDYNGYDSGKKIFIPEITMTQWAITASLQKLRVI
VANEKDNNILDISIHGMRADVKTSYYTTEVSITITDMRIQDPSPLAVYKK
IIWIERDESIFSMQVALFNQGTSTPGYELDLSRVDHMVNIEVGKIKIIFL
YNFVLRIQTFLNSFQESLQTVANKAQEYSEAAANKITNVLEEPPSSRTKL
DINVKAPVIYIPENSKSVNALMVDLGALTFKNDFAIDQSTSVVLEESKIT
LSNLRVSRSILLENTVKNEKGILHPINIFMDMTRNLSWNQDHTIPQISIA
ARMTSISVGMSQGDYNMFQLVLQSNFNEAINQVPSVPESKEEVKSRKILP
KDDKSGSARTASDESITVQFGFKMEFLDVELFKGDANPNEWASEMPPDKC
LTKMKCENLSVEGSMHLNSAMEVSVRLSNINVLDTQLDSAKKINELIRNI
KNDSSDFISIKFIQDKNSNSKLIVDIAGIYMCICVDYLMMLTDFFVKAQP
PPIQPPKKAMVPVRGYEKNPRKYRKKPLASPVEPRLADFEIDVKVARPEI
LLVEDMLNPDSNAIMVQTNFVLKYSIMKESDIINVDISDFQAVFCPFRSE
SRKKNTMDIISPTSISLYCNKPINNGMQGSLTMEHLIINITPAVIQSISK
IMNTLTAQSTDETSTRDGSFINNQDQLIVDNFWEKREFDENEFLFISKGL
MEKEKINTRNDGVESGFDVAQILLDVEQRRADQKFDNGDNLDEHKESKIY
LRMPDIKVILESEVAKKCVPMLIFECSMDGDLKNPLTQICLGVTVSVSVS
YFNDHINVWEPLLEVYPEEHNRQYSFEIEYISCDDSGLFDEEDFEDLISI
EPSAISILLLSRDALELTVSKSALDVLSNLGKAFEDAYSLHLEQHKSIFG
APYVVKNTCGKDVVFYINKEFDLKPCNSDEEFKVYLKADGEVQLYDAVDE
KQSLITEEKQAKIVKFGFADSISREYLSIYRTEVRALLFSPKDNEGPSRG
NVSEREINTLIVSTTAYLGMKQLELRSVLQVFNNTDDVITLYYLRKKSDK
SETAKNYLLLEEVLGGKCYNVPIPTLLTSNGKIYISVKQLNTEISNSGAL
WNIGKNAQPSDTQILLCKSNTGVMYYYSVALSIEEVQIVNSSETLRTYKL
DINVPLALHNQLPIKISYQSQIFSHDIKPGCLELVRTACLKVQSNIKVTY
HYQKKEWFGSLDLGPKMEELSVINFKAKEGYSESFMDLGVHLAIKNGQAN
VTIYSPYWMLNKTGYTLTYKAGDDCQVEHDAANTDAVLFSFLSKSIFSKK
KETISQAIVKKSDSGYSLQSLDTVESPAEDLHDHLDQTSTASINNVNNKN
QKFAKTVKAHLKINDSLWSDKFSLDTVGSSGKVMCKKKPVTYEVGVKINL
SSSGLTKIVTFLPYYMILNQSSIVLYCNELVENAGWIKVPPGEAVPFWPR
SEVGKSMMMRCKVEDREVLVTQPFPFYESHSILMKLEKKFVGIFVEVQIT
EEANLINLQLYQEGMALVKLINHLGDDVEIHFKQYNTPQELILPAYQTVM
YAWQDGRQKREFIWRVEGDKKQHSNNLTSDGIEDFYINEMKAYWVSFVWD
SNRILLFTNDIHVAKHARLSADLESIDQEITISLHSIGLSLVDNSLQQEL
AYISITSSGVKWNQIRRKNRHKPLKLAQSEALEAAFDEFVNRLKTSEAEV
EEHIVKVEGLEVDLSKKLMIKPEKCELSRVFEPGVFLQYRTSPHQVHVHL
RIYKIQIDNQMSDCNFPVVFAPFPPPRSVALDTGLKPLIELSAVIIKSGE
TGAMRYKYFRVLIQEMQLMLDQGFVNSILDFVSGSVPTYDEEAMFVKDCQ
MIRASLIETSTIKTILSGGKKCVFDNFHISPIKIHLSFSLSRVNRTGKTA
LHSEVLNLFLQSVGIAITDMQDVIFKLGYFEKQNSIMPMNQMVSSITSHY
VSQALRQMYVLVLGLDVLGNPFGVIRGMAQGVEDLFYEPVRGAVLGPEEF
VEGVAYGVKSLFGHAVGGAAGAVSRIVGTMGKGIAALTLDADYQRKRREQ
LAKRPENLGVGLAQGGKGLIVGFVQGVTGVVTKPIDGAKKDGIEGFFKGV
GKGLVGVVTRPVSGVVDFASSSFEGIRRVADTNIEVFRIRPPRFIAKDGI
IRSYGLREAEGNNIWIKITEKCDPIPNELYVFHVYCKSKKNVIFLTNQRI
FHLDRNEILGIWSIQWQIAYEKLDGPPTINESKHTIVFTLKEKQRKLFSK
DNITKEIEADLIVLKAVVSKIIKIQKYFEDKKQYSNIS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
GO:0005622intracellular
GO:0005829cytosol
GO:0031045dense core granule
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000099neuron
PLANA:0000484secretory system
PLANA:0002075pigment cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR026847VPS13
IPR031645VPS13_C
IPR026854VPS13_N
IPR031642VPS13_mid_rpt
IPR015412Autophagy-rel_C
IPR009543SHR-BD
IPR031646VPS13_N2
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
Vocabulary: biological process
TermDefinition
GO:0006810transport
GO:0006895Golgi to endosome transport
GO:0006914autophagy
GO:0007399nervous system development
GO:0007626locomotory behavior
GO:0008104protein localization
GO:0015031protein transport
GO:0035176social behavior
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 403..423
NoneNo IPR availableCOILSCoilCoilcoord: 2793..2813
NoneNo IPR availableCOILSCoilCoilcoord: 1060..1090
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1439..1459
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1434..1462
NoneNo IPR availablePANTHERPTHR16166:SF69VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 13Ccoord: 2451..3357
NoneNo IPR availablePANTHERPTHR16166:SF69VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 13Ccoord: 1139..2426
coord: 1..112
coord: 854..1069
NoneNo IPR availablePANTHERPTHR16166:SF69VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 13Ccoord: 121..822
IPR026854Vacuolar protein sorting-associated protein 13, N-terminal domainPFAMPF12624Chorein_Ncoord: 3..116
e-value: 6.1E-35
score: 119.8
IPR015412Autophagy-related, C-terminalPFAMPF09333ATG_Ccoord: 3150..3231
e-value: 4.8E-8
score: 33.2
IPR031645Vacuolar protein sorting-associated protein 13, C-terminalPFAMPF16909VPS13_Ccoord: 2974..3145
e-value: 3.0E-57
score: 193.3
IPR009543Vacuolar protein sorting-associated protein 13, SHR-binding domainPFAMPF06650SHR-BDcoord: 2350..2655
e-value: 1.7E-23
score: 83.4
IPR031646Vacuolar protein sorting-associated protein 13, second N-terminal domainPFAMPF16908VPS13coord: 138..371
e-value: 4.5E-64
score: 216.5
IPR031642VPS13, repeated coiled regionPFAMPF16910VPS13_mid_rptcoord: 1233..1345
e-value: 4.5E-8
score: 32.8
coord: 573..804
e-value: 3.0E-61
score: 206.9
IPR026847Vacuolar protein sorting-associated protein 13PANTHERPTHR16166VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13coord: 121..822
IPR026847Vacuolar protein sorting-associated protein 13PANTHERPTHR16166VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13coord: 2451..3357
coord: 1..112
IPR026847Vacuolar protein sorting-associated protein 13PANTHERPTHR16166VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13coord: 1139..2426
coord: 854..1069