Ragulator complex protein LAMTOR2

Overview
NameRagulator complex protein LAMTOR2
Smed IDSMED30001499
Length (bp)565
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Ragulator complex protein LAMTOR2 (SMED30001499) t-SNE clustered cells

Violin plots show distribution of expression levels for Ragulator complex protein LAMTOR2 (SMED30001499) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ragulator complex protein LAMTOR2 (SMED30001499) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ragulator complex protein LAMTOR2 (SMED30001499) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30001499

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30001499h1SMcG0009678 PL030014A20A02smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30001499h1SMcG0009678 Contig623GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30001499h1SMcG0009678 dd_Smed_v4_2137_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30001499h1SMcG0009678 dd_Smed_v4_2137_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Human
Match: LAMTOR2 (late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 [Source:HGNC Symbol;Acc:HGNC:29796])

HSP 1 Score: 88.5817 bits (218), Expect = 2.361e-22
Identity = 45/120 (37.50%), Postives = 69/120 (57.50%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKL 453
            RPKAL   L+     GV S +L  +EG LLA S       +  AAI +NIW  Y R  +    +D ++ +L++   G++AI++V N+LLC+   E   FGM++AK   L+  L EPL+++
Sbjct:    3 RPKALTQVLSQANTGGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDRNGNQAFNEDNLKFILMDCMEGRVAITRVANLLLCMYAKETVGFGMLKAKAQALVQYLEEPLTQV 122
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Human
Match: LAMTOR2 (late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 [Source:HGNC Symbol;Acc:HGNC:29796])

HSP 1 Score: 80.1073 bits (196), Expect = 9.033e-19
Identity = 47/123 (38.21%), Postives = 67/123 (54.47%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKLTGF 462
            RPKAL   L+     GV S +L  +EG LLA S       +  AAI +NIW  Y R  +    +D ++ +L++   G++AI++V N+LLC+   E   FGM++AK V   D L  P S   GF
Sbjct:    3 RPKALTQVLSQANTGGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDRNGNQAFNEDNLKFILMDCMEGRVAITRVANLLLCMYAKETVGFGMLKAKAVCGTD-LCSP-SAGPGF 123
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Human
Match: LAMTOR2 (late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 [Source:HGNC Symbol;Acc:HGNC:29796])

HSP 1 Score: 49.2914 bits (116), Expect = 9.712e-8
Identity = 26/73 (35.62%), Postives = 38/73 (52.05%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIE 312
            RPKAL   L+     GV S +L  +EG LLA S       +  AAI +NIW  Y R  +    +D ++ +L++
Sbjct:    3 RPKALTQVLSQANTGGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDRNGNQAFNEDNLKFILMD 75
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Celegans
Match: lmtr-2 (Ragulator complex protein LAMTOR2 homolog [Source:UniProtKB/Swiss-Prot;Acc:Q9N2U6])

HSP 1 Score: 53.9138 bits (128), Expect = 1.570e-9
Identity = 38/123 (30.89%), Postives = 65/123 (52.85%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLA-----QSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVG-NVLLCVCGSENSEFGMIRAKMVTLIDRLTEPL 444
            + KAL   L     +GVD   LF  EG+LLA     Q  V SN     +A++A++W   +R+ ++      ++E ++  E G +  + V   +LL V   ++++ GM+RAK+ TL   L +P+
Sbjct:    3 KQKALVDVLGQVNTSGVDGSWLFNKEGLLLAYVGSEQKAVASN---VSSALIASVWAALERRAND------LKETILVLENGVIGCTLVARTMLLAVKADKSADLGMVRAKLHTLAAYLEQPI 116
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Celegans
Match: lmtr-2 (Ragulator complex protein LAMTOR2 homolog [Source:UniProtKB/Swiss-Prot;Acc:Q9N2U6])

HSP 1 Score: 53.9138 bits (128), Expect = 1.570e-9
Identity = 38/123 (30.89%), Postives = 65/123 (52.85%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLA-----QSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVG-NVLLCVCGSENSEFGMIRAKMVTLIDRLTEPL 444
            + KAL   L     +GVD   LF  EG+LLA     Q  V SN     +A++A++W   +R+ ++      ++E ++  E G +  + V   +LL V   ++++ GM+RAK+ TL   L +P+
Sbjct:    3 KQKALVDVLGQVNTSGVDGSWLFNKEGLLLAYVGSEQKAVASN---VSSALIASVWAALERRAND------LKETILVLENGVIGCTLVARTMLLAVKADKSADLGMVRAKLHTLAAYLEQPI 116
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Fly
Match: CG5189 (gene:FBgn0034350 transcript:FBtr0299805)

HSP 1 Score: 77.411 bits (189), Expect = 2.007e-18
Identity = 39/121 (32.23%), Postives = 68/121 (56.20%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKLT 456
            +PKAL   L+     GV++ +L + EG LLA S       +  AAI +NIW  Y++   N   + ++  +LI+ E G +AI++V +VLLC+   +    G+++ K ++L   L  PL +++
Sbjct:    3 KPKALTQVLSQANTGGVENTLLLSQEGALLAYSGYGDKDARITAAIASNIWAAYEKHGRNAFREGRLTFVLIDCENGHVAITQVASVLLCLYAKQTVGLGLLKQKAMSLASYLERPLKQIS 123
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Fly
Match: CG5189 (gene:FBgn0034350 transcript:FBtr0086694)

HSP 1 Score: 77.411 bits (189), Expect = 2.007e-18
Identity = 39/121 (32.23%), Postives = 68/121 (56.20%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKLT 456
            +PKAL   L+     GV++ +L + EG LLA S       +  AAI +NIW  Y++   N   + ++  +LI+ E G +AI++V +VLLC+   +    G+++ K ++L   L  PL +++
Sbjct:    3 KPKALTQVLSQANTGGVENTLLLSQEGALLAYSGYGDKDARITAAIASNIWAAYEKHGRNAFREGRLTFVLIDCENGHVAITQVASVLLCLYAKQTVGLGLLKQKAMSLASYLERPLKQIS 123
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Zebrafish
Match: lamtor2 (late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 [Source:NCBI gene;Acc:445155])

HSP 1 Score: 87.4261 bits (215), Expect = 4.121e-22
Identity = 44/122 (36.07%), Postives = 70/122 (57.38%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKLTG 459
            RPKAL   L+    +GV S +L  +EG LLA S       +  AAI +NIW  Y +       +D+++ +L++   G++AI++V N+LLC+   E   FGM++AK   L+  L EPL+++  
Sbjct:    3 RPKALTQVLSQANTSGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDKNGHQAFNEDKLKFILMDCMEGRVAITRVANLLLCMYAKETVGFGMLKAKAEALVQYLEEPLTQVAA 124
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Xenopus
Match: lamtor2 (late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 [Source:NCBI gene;Acc:448570])

HSP 1 Score: 85.5001 bits (210), Expect = 3.798e-21
Identity = 44/121 (36.36%), Postives = 69/121 (57.02%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKLT 456
            RPKAL   L+     GV S +L  +EG LLA S       +  AAI +NIW  Y +       +D ++ +L++   G++AI++V N+LLC+   E   FGM++AK   L+  L EPL++++
Sbjct:    3 RPKALTQVLSQANTGGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDKNGHQAFNEDNLKFILMDCMEGRVAITRVSNLLLCMYAKETVGFGMLKAKAQALVYYLEEPLNQVS 123
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Xenopus
Match: lamtor2 (late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 [Source:NCBI gene;Acc:448570])

HSP 1 Score: 47.3654 bits (111), Expect = 5.004e-7
Identity = 25/73 (34.25%), Postives = 37/73 (50.68%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIE 312
            RPKAL   L+     GV S +L  +EG LLA S       +  AAI +NIW  Y +       +D ++ +L++
Sbjct:    3 RPKALTQVLSQANTGGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDKNGHQAFNEDNLKFILMD 75
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Mouse
Match: Lamtor2 (late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 [Source:MGI Symbol;Acc:MGI:1932697])

HSP 1 Score: 88.5817 bits (218), Expect = 1.425e-22
Identity = 45/120 (37.50%), Postives = 69/120 (57.50%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKL 453
            RPKAL   L+     GV S +L  +EG LLA S       +  AAI +NIW  Y R  +    +D ++ +L++   G++AI++V N+LLC+   E   FGM++AK   L+  L EPL+++
Sbjct:    3 RPKALTQVLSQANTGGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDRNGNQAFNEDSLKFILMDCMEGRVAITRVANLLLCMYAKETVGFGMLKAKAQALVQYLEEPLTQV 122
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Mouse
Match: Lamtor2 (late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 [Source:MGI Symbol;Acc:MGI:1932697])

HSP 1 Score: 44.2838 bits (103), Expect = 1.938e-6
Identity = 19/46 (41.30%), Postives = 31/46 (67.39%), Query Frame = 1 
Query:  322 GKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKLTG 459
            G++AI++V N+LLC+   E   FGM++AK   L+  L EPL+++  
Sbjct:    6 GRVAITRVANLLLCMYAKETVGFGMLKAKAQALVQYLEEPLTQVAA 51
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. UniProt/SwissProt
Match: sp|Q9JHS3|LTOR2_MOUSE (Ragulator complex protein LAMTOR2 OS=Mus musculus OX=10090 GN=Lamtor2 PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 9.967e-22
Identity = 45/120 (37.50%), Postives = 69/120 (57.50%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKL 453
            RPKAL   L+     GV S +L  +EG LLA S       +  AAI +NIW  Y R  +    +D ++ +L++   G++AI++V N+LLC+   E   FGM++AK   L+  L EPL+++
Sbjct:    3 RPKALTQVLSQANTGGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDRNGNQAFNEDSLKFILMDCMEGRVAITRVANLLLCMYAKETVGFGMLKAKAQALVQYLEEPLTQV 122
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. UniProt/SwissProt
Match: sp|Q9Y2Q5|LTOR2_HUMAN (Ragulator complex protein LAMTOR2 OS=Homo sapiens OX=9606 GN=LAMTOR2 PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.134e-21
Identity = 45/120 (37.50%), Postives = 69/120 (57.50%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKL 453
            RPKAL   L+     GV S +L  +EG LLA S       +  AAI +NIW  Y R  +    +D ++ +L++   G++AI++V N+LLC+   E   FGM++AK   L+  L EPL+++
Sbjct:    3 RPKALTQVLSQANTGGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDRNGNQAFNEDNLKFILMDCMEGRVAITRVANLLLCMYAKETVGFGMLKAKAQALVQYLEEPLTQV 122
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. UniProt/SwissProt
Match: sp|Q3T132|LTOR2_BOVIN (Ragulator complex protein LAMTOR2 OS=Bos taurus OX=9913 GN=LAMTOR2 PE=2 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.134e-21
Identity = 45/120 (37.50%), Postives = 69/120 (57.50%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKL 453
            RPKAL   L+     GV S +L  +EG LLA S       +  AAI +NIW  Y R  +    +D ++ +L++   G++AI++V N+LLC+   E   FGM++AK   L+  L EPL+++
Sbjct:    3 RPKALTQVLSQANTGGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDRNGNQAFNEDNLKFILMDCMEGRVAITRVANLLLCMYAKETVGFGMLKAKAQALVQYLEEPLTQV 122
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. UniProt/SwissProt
Match: sp|B5X7X4|LTOR2_SALSA (Ragulator complex protein LAMTOR2 OS=Salmo salar OX=8030 GN=lamtor2 PE=2 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 5.495e-21
Identity = 44/122 (36.07%), Postives = 71/122 (58.20%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKLTG 459
            RPKAL   L+    +GV S +L  +EG LLA S       +  AAI +NIW+ Y +       +D+++ +L++   G++AI++V N+LLC+   E   FGM++AK   L+  L EPL+++  
Sbjct:    3 RPKALTQVLSQANTSGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWSAYDKNGHQAFNEDKLKFILMDCMEGRVAITRVANLLLCMYAKETVGFGMLKAKAEALVLYLEEPLTQVAA 124
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. UniProt/SwissProt
Match: sp|Q7ZXB7|LTR2B_XENLA (Ragulator complex protein LAMTOR2-B OS=Xenopus laevis OX=8355 GN=lamtor2-b PE=2 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 8.905e-21
Identity = 44/121 (36.36%), Postives = 69/121 (57.02%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKLT 456
            RPKAL   L+     GV S +L  +EG LLA S       +  AAI +NIW  Y +       +D ++ +L++   G++AI++V N+LLC+   E   FGM++AK   L+  L EPL++++
Sbjct:    3 RPKALTQVLSQANTGGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDKNGHQAFNEDNLKFILMDCMEGRVAITRVSNLLLCMYAKETVGFGMLKAKAQALVHYLEEPLNQVS 123
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. TrEMBL
Match: A0A5K4FCG2 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 2.516e-29
Identity = 54/118 (45.76%), Postives = 82/118 (69.49%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLS 447
            RP+AL S L      G+ S++LF  EG+LLA +++  + E++KAAI AN+WNIYQRQ ++ +  D +QE++++   GKL I++V +VLLC+  S++   GM+RAKM  L+  L EPLS
Sbjct:    3 RPRALTSALRKMNTGGIQSVMLFNPEGVLLAYTSLAGDSERSKAAIAANVWNIYQRQLES-SESDTVQEIILKLSEGKLIITRVASVLLCLHASKDVGLGMLRAKMNALVQNLQEPLS 119
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. TrEMBL
Match: A0A3P8J2D1 (Robl_LC7 domain-containing protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS3575 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 4.910e-29
Identity = 55/118 (46.61%), Postives = 82/118 (69.49%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLS 447
            RP+AL S L      G+ S++LF  EG+LLA +++  + E++KAAI ANIWNIYQRQ ++ +  D +QE+++E   G+L I++V +VLLC+  S++   GM+RAKM  L+  L EPLS
Sbjct:   30 RPRALTSALRKMNTGGMQSVMLFNPEGVLLAYTSLAGDSERSKAAIAANIWNIYQRQLES-SESDTVQEIILELSEGRLIITRVASVLLCLHASKDVGLGMLRAKMNALVQNLQEPLS 146
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. TrEMBL
Match: A0A183VSW9 (Robl_LC7 domain-containing protein OS=Trichobilharzia regenti OX=157069 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 5.240e-29
Identity = 55/118 (46.61%), Postives = 82/118 (69.49%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLS 447
            RP+AL S L      G+ S++LF  EG+LLA +++  + E++KAAI ANIWNIYQRQ ++ +  D +QE+++E   G+L I++V +VLLC+  S++   GM+RAKM  L+  L EPLS
Sbjct:   30 RPRALTSALRKMNTGGMQSVMLFNPEGVLLAYTSLAGDSERSKAAIAANIWNIYQRQLES-SESDTVQEIILELSEGRLIITRVASVLLCLHASKDVGLGMLRAKMNALVQNLQEPLS 146
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. TrEMBL
Match: A0A0X3PXK3 (Ragulator complex protein LAMTOR2 OS=Schistocephalus solidus OX=70667 GN=LTOR2 PE=4 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 7.309e-29
Identity = 57/121 (47.11%), Postives = 85/121 (70.25%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKLT 456
            RP+AL S L      GV SI+LF++EG+LL  + +    EKTKAAIVANIWNIYQ+Q + ++  + +QE++IE   G+L  +KV +VL+C+ GS++   G++RAK+ +L++ L  PLS LT
Sbjct:    3 RPRALASALAKVNTGGVQSIMLFSTEGVLLTHTGIIDENEKTKAAIVANIWNIYQKQLE-LSECETVQEIIIELTHGRLVFTKVASVLICIHGSKDIGLGLLRAKINSLVENLQGPLSFLT 122
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. TrEMBL
Match: A0A3P7NMI1 (Robl_LC7 domain-containing protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS6350 PE=4 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 3.025e-28
Identity = 56/121 (46.28%), Postives = 84/121 (69.42%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKLT 456
            RP+AL S L      GV SI+LF++EG+LL  + V    EKTKAAIVANIWNIYQ+Q + ++  + +QE++IE   G+L  +KV ++L+C+ GS++   G++R K+ +L++ L  PLS LT
Sbjct:    3 RPRALASALAKVNTGGVQSIMLFSTEGVLLTHTGVIDENEKTKAAIVANIWNIYQKQLE-LSECETVQEIIIELTRGRLVFTKVASILICIHGSKDIGLGLLRTKINSLVENLQGPLSFLT 122
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BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Cavefish
Match: lamtor2 (late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 [Source:NCBI gene;Acc:103042042])

HSP 1 Score: 87.0409 bits (214), Expect = 4.667e-22
Identity = 44/122 (36.07%), Postives = 70/122 (57.38%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKLTG 459
            RPKAL   L+     GV S +L  +EG LLA S       +  AAI +NIW  Y +       +D+++ +L++   G++AI++V N+LLC+   E   FGM++AK   L++ L EPL+++  
Sbjct:    3 RPKALTQVLSQANTNGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDKNGHQAFNEDKLKFILMDCMEGRVAITRVANLLLCMYAKETVGFGMLKAKAEALVEYLQEPLTQVAA 124
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Cavefish
Match: lamtor2 (late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 [Source:NCBI gene;Acc:103042042])

HSP 1 Score: 46.9802 bits (110), Expect = 3.966e-7
Identity = 25/73 (34.25%), Postives = 38/73 (52.05%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIE 312
            RPKAL   L+     GV S +L  +EG LLA S       +  AAI +NIW  Y +       +D+++ +L++
Sbjct:    3 RPKALTQVLSQANTNGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDKNGHQAFNEDKLKFILMD 75
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BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Nematostella
Match: EDO38060 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDX4])

HSP 1 Score: 74.7146 bits (182), Expect = 9.058e-18
Identity = 42/121 (34.71%), Postives = 72/121 (59.50%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTK-AAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKL 453
            +PK L   L+     GV S +L  +EG +L+ S  N + E  K AAI ++IW+ Y++       +++I+ L ++ E GK+ I++V N+L+C+   E+  FGM++AK   L+  L EPL ++
Sbjct:    3 KPKVLTEVLSQANTGGVISTLLLNNEGSILSYSG-NGDKEANKTAAIASSIWSAYEKNGRIAFHNEEIKMLFMDCEEGKVVIARVANLLVCLYAKESVGFGMLKAKAEALVSYLEEPLRQV 122
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Medaka
Match: lamtor2 (late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 [Source:NCBI gene;Acc:101171523])

HSP 1 Score: 85.1149 bits (209), Expect = 2.499e-21
Identity = 44/122 (36.07%), Postives = 68/122 (55.74%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKLTG 459
            RPKAL   L+     GV S +L  +EG LLA S       +  AAI +NIW  Y +       +D+++ +L++   G++AI+ V N+LLC+   E   FGM++AK   L+  L EPL+++  
Sbjct:    3 RPKALTQVLSQANTNGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDKNGHQAFNEDKLKFILMDCMEGRVAITHVANLLLCMYAKETVGFGMLKAKAEALVQYLEEPLTQVAA 124
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Medaka
Match: lamtor2 (late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 [Source:NCBI gene;Acc:101171523])

HSP 1 Score: 76.6406 bits (187), Expect = 2.274e-17
Identity = 40/113 (35.40%), Postives = 63/113 (55.75%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRL 432
            RPKAL   L+     GV S +L  +EG LLA S       +  AAI +NIW  Y +       +D+++ +L++   G++AI+ V N+LLC+   E   FGM++AK+  L+  +
Sbjct:    3 RPKALTQVLSQANTNGVQSTLLLNNEGSLLAYSGYGDTDARVTAAIASNIWAAYDKNGHQAFNEDKLKFILMDCMEGRVAITHVANLLLCMYAKETVGFGMLKAKVRALLPHM 115
[Close]
BLAST of Ragulator complex protein LAMTOR2 vs. Planmine SMEST
Match: SMESG000051838.1 (SMESG000051838.1)

HSP 1 Score: 251.906 bits (642), Expect = 4.393e-87
Identity = 123/123 (100.00%), Postives = 123/123 (100.00%), Query Frame = 1 
Query:   94 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKLTGF 462
            RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKLTGF
Sbjct:    3 RPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVANIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSEFGMIRAKMVTLIDRLTEPLSKLTGF 125
The following BLAST results are available for this feature:
BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
LAMTOR22.361e-2237.50late endosomal/lysosomal adaptor, MAPK and MTOR ac... [more]
LAMTOR29.033e-1938.21late endosomal/lysosomal adaptor, MAPK and MTOR ac... [more]
LAMTOR29.712e-835.62late endosomal/lysosomal adaptor, MAPK and MTOR ac... [more]
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BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
lmtr-21.570e-930.89Ragulator complex protein LAMTOR2 homolog [Source... [more]
lmtr-21.570e-930.89Ragulator complex protein LAMTOR2 homolog [Source... [more]
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BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG51892.007e-1832.23gene:FBgn0034350 transcript:FBtr0299805[more]
CG51892.007e-1832.23gene:FBgn0034350 transcript:FBtr0086694[more]
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BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
lamtor24.121e-2236.07late endosomal/lysosomal adaptor, MAPK and MTOR ac... [more]
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BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
lamtor23.798e-2136.36late endosomal/lysosomal adaptor, MAPK and MTOR ac... [more]
lamtor25.004e-734.25late endosomal/lysosomal adaptor, MAPK and MTOR ac... [more]
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BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Lamtor21.425e-2237.50late endosomal/lysosomal adaptor, MAPK and MTOR ac... [more]
Lamtor21.938e-641.30late endosomal/lysosomal adaptor, MAPK and MTOR ac... [more]
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BLAST of Ragulator complex protein LAMTOR2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9JHS3|LTOR2_MOUSE9.967e-2237.50Ragulator complex protein LAMTOR2 OS=Mus musculus ... [more]
sp|Q9Y2Q5|LTOR2_HUMAN1.134e-2137.50Ragulator complex protein LAMTOR2 OS=Homo sapiens ... [more]
sp|Q3T132|LTOR2_BOVIN1.134e-2137.50Ragulator complex protein LAMTOR2 OS=Bos taurus OX... [more]
sp|B5X7X4|LTOR2_SALSA5.495e-2136.07Ragulator complex protein LAMTOR2 OS=Salmo salar O... [more]
sp|Q7ZXB7|LTR2B_XENLA8.905e-2136.36Ragulator complex protein LAMTOR2-B OS=Xenopus lae... [more]
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BLAST of Ragulator complex protein LAMTOR2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5K4FCG22.516e-2945.76Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A3P8J2D14.910e-2946.61Robl_LC7 domain-containing protein OS=Trichobilhar... [more]
A0A183VSW95.240e-2946.61Robl_LC7 domain-containing protein OS=Trichobilhar... [more]
A0A0X3PXK37.309e-2947.11Ragulator complex protein LAMTOR2 OS=Schistocephal... [more]
A0A3P7NMI13.025e-2846.28Robl_LC7 domain-containing protein OS=Dibothriocep... [more]
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BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
lamtor24.667e-2236.07late endosomal/lysosomal adaptor, MAPK and MTOR ac... [more]
lamtor23.966e-734.25late endosomal/lysosomal adaptor, MAPK and MTOR ac... [more]
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BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO380609.058e-1834.71Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Ragulator complex protein LAMTOR2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
lamtor22.499e-2136.07late endosomal/lysosomal adaptor, MAPK and MTOR ac... [more]
lamtor22.274e-1735.40late endosomal/lysosomal adaptor, MAPK and MTOR ac... [more]
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BLAST of Ragulator complex protein LAMTOR2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000051838.14.393e-87100.00SMESG000051838.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001499 ID=SMED30001499|Name=Ragulator complex protein LAMTOR2|organism=Schmidtea mediterranea sexual|type=transcript|length=565bp
GTTTAAATAATCCATGTCATCATAATTTAATTTCTTTGTATTTCCTTAAT
TAAAATTTCTATTTTTTTGATTTTACTCTTGTAATTTATGCTTCGACCTA
AAGCACTTTGTAGTACTCTGAATCATTATGTTGGAGCAGGGGTAGATTCG
ATAATATTATTTACTTCAGAGGGTATATTATTAGCACAATCTAATGTTAA
TTCAAATGGCGAGAAAACCAAAGCCGCAATTGTTGCTAATATTTGGAATA
TATATCAAAGACAACAAGATAATGTGACAATTGATGATCAAATCCAAGAA
CTCTTAATAGAGTTTGAACTTGGTAAATTAGCTATTAGTAAAGTTGGTAA
TGTTTTACTATGTGTTTGTGGGTCCGAGAATTCAGAATTTGGAATGATAA
GAGCAAAAATGGTCACCTTAATAGACAGATTAACTGAACCTCTTAGTAAA
TTAACAGGATTTTAATTAACTTTATTTTCCTTGATTTACATTGTAATCTT
TCATAAAATTTTGATTTGTGTTTAAATTTCAAAGATTTATGATTAAATAA
ATTGCTAAATTGGCA
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protein sequence of SMED30001499-orf-1

>SMED30001499-orf-1 ID=SMED30001499-orf-1|Name=SMED30001499-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=126bp
MLRPKALCSTLNHYVGAGVDSIILFTSEGILLAQSNVNSNGEKTKAAIVA
NIWNIYQRQQDNVTIDDQIQELLIEFELGKLAISKVGNVLLCVCGSENSE
FGMIRAKMVTLIDRLTEPLSKLTGF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0060090binding, bridging
GO:0005085guanyl-nucleotide exchange factor activity
GO:0005515protein binding
GO:0032947protein complex scaffold
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR004942Roadblock/LAMTOR2_dom
Vocabulary: biological process
TermDefinition
GO:0000186activation of MAPKK activity
GO:0016049cell growth
GO:0032008positive regulation of TOR signaling
GO:0034613cellular protein localization
GO:0043547positive regulation of GTPase activity
GO:0071230cellular response to amino acid stimulus
Vocabulary: cellular component
TermDefinition
GO:0005764lysosome
GO:0005765lysosomal membrane
GO:0005768endosome
GO:0005770late endosome
GO:0016020membrane
GO:0031902late endosome membrane
GO:0070062extracellular exosome
GO:0071986Ragulator complex