tRNA pseudouridine synthase

Overview
NametRNA pseudouridine synthase
Smed IDSMED30001491
Length (bp)3878
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of tRNA pseudouridine synthase (SMED30001491) t-SNE clustered cells

Violin plots show distribution of expression levels for tRNA pseudouridine synthase (SMED30001491) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of tRNA pseudouridine synthase (SMED30001491) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for tRNA pseudouridine synthase (SMED30001491) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30001491

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30001491SMESG000041371.1 SmedASXL_075271SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30001491SMESG000041371.1 SmedASXL_016557SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30001491 SmedASXL_075272SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30001491SMESG000041371.1 dd_Smed_v4_16208_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30001491SMESG000041371.1 dd_Smed_v4_16208_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Human
Match: PUS3 (pseudouridine synthase 3 [Source:HGNC Symbol;Acc:HGNC:25461])

HSP 1 Score: 163.696 bits (413), Expect = 3.801e-42
Identity = 91/272 (33.46%), Postives = 138/272 (50.74%), Query Frame = 2 
Query:  731 IDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPE----HETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRH-----SKKIDSALLLNYIN-----------KDVRFENWKPLLERLKESTLEARI 1486
            I Y  ILN+ LP DI+ILAW+PV+  F A F+CL R+Y+YFFPR +L I  M  A  K +G+HDFRN C M V  G+  F R I S +++ + +   E       ++    ++G +F+YHQ+RC++ +L +IG G EK E       ++      KP Y MA E PLV ++  + ++ W   ++A      +L  LW     K  +L   L  L+          K+D       +            + V+   +KPL++R K   LE+RI
Sbjct:  158 IRYTHILNRVLPPDIRILAWAPVEPSFSARFSCLERTYRYFFPRADLDIVTMDYAAQKYVGTHDFRNLCKMDVANGVINFQRTILSAQVQLVGQSPGEGRWQEPFQLCQFEVTGQAFLYHQVRCMMAILFLIGQGMEKPE--IIDELLNIEKNPQKPQYSMAVEFPLVLYDCKFENVKWIYDQEAQEFNITHLQQLWANHAVKTHMLYSMLQGLDTVPVPCGIGPKMDGMTEWGNVKPSVIKQTSAFVEGVKMRTYKPLMDRPKCQGLESRI 427

HSP 2 Score: 86.6557 bits (213), Expect = 8.216e-17
Identity = 42/83 (50.60%), Postives = 55/83 (66.27%), Query Frame = 1 
Query:  160 KYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDV-NISFCGRTDKGVSAFQQVISLVVRS 405
            K + DFS +  RH+AL+  Y+GW Y G   Q  ++N ++ KL E+L K++L E R   N   CGRTDKGVSAF QVISL +RS
Sbjct:   52 KRAFDFSAHGRRHVALRIAYMGWGYQGFASQENTNNTIEEKLFEALTKTRLVESRQTSNYHRCGRTDKGVSAFGQVISLDLRS 134
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Human
Match: PUS3 (pseudouridine synthase 3 [Source:HGNC Symbol;Acc:HGNC:25461])

HSP 1 Score: 163.696 bits (413), Expect = 3.801e-42
Identity = 91/272 (33.46%), Postives = 138/272 (50.74%), Query Frame = 2 
Query:  731 IDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPE----HETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRH-----SKKIDSALLLNYIN-----------KDVRFENWKPLLERLKESTLEARI 1486
            I Y  ILN+ LP DI+ILAW+PV+  F A F+CL R+Y+YFFPR +L I  M  A  K +G+HDFRN C M V  G+  F R I S +++ + +   E       ++    ++G +F+YHQ+RC++ +L +IG G EK E       ++      KP Y MA E PLV ++  + ++ W   ++A      +L  LW     K  +L   L  L+          K+D       +            + V+   +KPL++R K   LE+RI
Sbjct:  158 IRYTHILNRVLPPDIRILAWAPVEPSFSARFSCLERTYRYFFPRADLDIVTMDYAAQKYVGTHDFRNLCKMDVANGVINFQRTILSAQVQLVGQSPGEGRWQEPFQLCQFEVTGQAFLYHQVRCMMAILFLIGQGMEKPE--IIDELLNIEKNPQKPQYSMAVEFPLVLYDCKFENVKWIYDQEAQEFNITHLQQLWANHAVKTHMLYSMLQGLDTVPVPCGIGPKMDGMTEWGNVKPSVIKQTSAFVEGVKMRTYKPLMDRPKCQGLESRI 427

HSP 2 Score: 86.6557 bits (213), Expect = 8.216e-17
Identity = 42/83 (50.60%), Postives = 55/83 (66.27%), Query Frame = 1 
Query:  160 KYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDV-NISFCGRTDKGVSAFQQVISLVVRS 405
            K + DFS +  RH+AL+  Y+GW Y G   Q  ++N ++ KL E+L K++L E R   N   CGRTDKGVSAF QVISL +RS
Sbjct:   52 KRAFDFSAHGRRHVALRIAYMGWGYQGFASQENTNNTIEEKLFEALTKTRLVESRQTSNYHRCGRTDKGVSAFGQVISLDLRS 134
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Human
Match: PUS3 (pseudouridine synthase 3 [Source:HGNC Symbol;Acc:HGNC:25461])

HSP 1 Score: 98.5969 bits (244), Expect = 7.706e-22
Identity = 62/221 (28.05%), Postives = 101/221 (45.70%), Query Frame = 2 
Query:  884 MQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPE----HETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRH-----SKKIDSALLLNYIN-----------KDVRFENWKPLLERLKESTLEARI 1486
            M  A  K +G+HDFRN C M V  G+  F R I S +++ + +   E       ++    ++G +F+YHQ+RC++ +L +IG G EK E       ++      KP Y MA E PLV ++  + ++ W   ++A      +L  LW     K  +L   L  L+          K+D       +            + V+   +KPL++R K   LE+RI
Sbjct:    1 MDYAAQKYVGTHDFRNLCKMDVANGVINFQRTILSAQVQLVGQSPGEGRWQEPFQLCQFEVTGQAFLYHQVRCMMAILFLIGQGMEKPE--IIDELLNIEKNPQKPQYSMAVEFPLVLYDCKFENVKWIYDQEAQEFNITHLQQLWANHAVKTHMLYSMLQGLDTVPVPCGIGPKMDGMTEWGNVKPSVIKQTSAFVEGVKMRTYKPLMDRPKCQGLESRI 219
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Human
Match: PUS3 (pseudouridine synthase 3 [Source:HGNC Symbol;Acc:HGNC:25461])

HSP 1 Score: 87.8113 bits (216), Expect = 3.819e-19
Identity = 42/83 (50.60%), Postives = 55/83 (66.27%), Query Frame = 1 
Query:  160 KYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDV-NISFCGRTDKGVSAFQQVISLVVRS 405
            K + DFS +  RH+AL+  Y+GW Y G   Q  ++N ++ KL E+L K++L E R   N   CGRTDKGVSAF QVISL +RS
Sbjct:   52 KRAFDFSAHGRRHVALRIAYMGWGYQGFASQENTNNTIEEKLFEALTKTRLVESRQTSNYHRCGRTDKGVSAFGQVISLDLRS 134
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Human
Match: PUS3 (pseudouridine synthase 3 [Source:HGNC Symbol;Acc:HGNC:25461])

HSP 1 Score: 54.6842 bits (130), Expect = 2.295e-8
Identity = 22/53 (41.51%), Postives = 34/53 (64.15%), Query Frame = 1 
Query:  160 KYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTE 318
            K + DFS +  RH+AL+  Y+GW Y G   Q  ++N ++ KL E+L K++L E
Sbjct:   52 KRAFDFSAHGRRHVALRIAYMGWGYQGFASQENTNNTIEEKLFEALTKTRLVE 104
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Celegans
Match: tag-124 (Probable tRNA pseudouridine synthase tag-124 [Source:UniProtKB/Swiss-Prot;Acc:Q09524])

HSP 1 Score: 123.635 bits (309), Expect = 9.740e-30
Identity = 69/217 (31.80%), Postives = 116/217 (53.46%), Query Frame = 2 
Query:  725 KEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCT--MLVKQGITQFTRNIYSVKIEPLDEIEPEHET-KMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYN--DMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSEL 1360
            +E+ YV +LN  LP+ I++ AW+PV + F+A F+C  R+YKY F + +L++E M++    L+G HDF NFC   M  K+ +  + R +Y VK+E +    PE++   M  + +SGS F++H IR IV +L  I  G+E  +       +D      +P Y +A + PL   +  Y   D++W+  +         L   W  +  +  +++  L EL
Sbjct:  128 EELPYVKMLNGVLPKTIRVFAWAPVAQTFNARFDCNRRTYKYSFAKADLNLEKMRQGAELLVGEHDFSNFCQIDMNEKRLLQSYVRKVYEVKVEQV-STHPENDMYSMVELTVSGSGFLWHMIRYIVTILQEI--GRENEQPSLISQLLDLKKYPSRPQYTLASDTPLCLFDCGYKSEDVEWKVHDYTLKSTVTGLQKTWATYQARSRMMENMLGEL 341

HSP 2 Score: 80.8777 bits (198), Expect = 1.570e-15
Identity = 37/81 (45.68%), Postives = 55/81 (67.90%), Query Frame = 1 
Query:  166 SLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTED-RDVNISFCGRTDKGVSAFQQVISLVVRS 405
            +LDF  +  R IA++F YLGW + G++ Q  + N V++ + ++L K+ L ED    + S CGRTDKGVSAF+Q  ++VVRS
Sbjct:   21 TLDFLAHPRRKIAIQFFYLGWEHDGLVQQPHTQNTVENHIMQALIKTHLIEDWTKCDFSRCGRTDKGVSAFKQTAAMVVRS 101
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Celegans
Match: pus-1 (tRNA pseudouridine synthase [Source:UniProtKB/TrEMBL;Acc:B1V8I7])

HSP 1 Score: 50.8322 bits (120), Expect = 5.498e-6
Identity = 41/145 (28.28%), Postives = 62/145 (42.76%), Query Frame = 2 
Query:  746 ILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFP-------------RGNLSIELMQEAGTKL---IGSHDFRNFCTMLVKQGITQFTRNIYSVKI---EPL---DEIEPEHETKMYGIYISGSSFIYHQIRCIVGVLLMI 1114
            ILN  LP DI++         FH    C  R+Y Y  P                LS E + E  + L   +G+H+F N+     K+     + N Y +     EP    D+   E + +   I I G SF+ HQIR +VG+++ +
Sbjct:  120 ILNNILPADIRVFGMRRTTNFFHPQKQCDHRTYSYTCPSFVFAKPTELTNSSFRLSPETLAEVNSILSIYLGTHNFFNYT---AKRAYDDMSSNRYIISFECKEPFLFRDDFRKE-DVEFVQIVIKGQSFVLHQIRKMVGMVITV 260
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Fly
Match: CG3045 (gene:FBgn0034703 transcript:FBtr0071787)

HSP 1 Score: 138.658 bits (348), Expect = 5.115e-34
Identity = 98/320 (30.63%), Postives = 155/320 (48.44%), Query Frame = 2 
Query:  728 EIDYVFILNKFLPEDIKILAWSPVDKD-FHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHETKMYGIY---ISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYND------------------MDWQTSEQASNIINL----------------NLHSL-------WLKFYTKQTILKCFLSEL------NQRHSKKIDSALLLNYINKDVRFENWKPLLERLKESTLEARID----KHNILMKVEID 1522
            EIDY  +LN+ LP++I+ +AW P+    + A F+C+ R+Y+Y+FP+G+L I  M++A   L+   DFRNFC M V  G+T + RN+ S ++E  D+    H    Y +Y   I  ++F++HQIRCI+ VLL++G  KE          +D      KP Y  A  LPL      + D                  MD  T+   SN +N                 NL  L       W +F  K T+++  L +L      N +  +K+ + ++L  +   V    ++PLLER +  +LE RI+    K  +++K E +
Sbjct:  186 EIDYCGLLNRVLPKNIQCVAWMPLRSPVYSARFDCVSRTYRYYFPKGDLDIAAMRKACDLLVRHADFRNFCKMDVHNGVTNYMRNLQSARVEACDQT--NHTNSGYDMYYLEIQANAFLWHQIRCIMAVLLLVGQKKEN--PGVISDLLDVESNPCKPQYTPAIGLPLNLFRCDFRDHTTRSVNHPSSGDADEEAMD--TAADESNDLNAPEHLERDLTAWIYNEENLQKLIENTQCEWTQFSVKSTMIRNVLQQLENLFEENFKPKEKVLAQVIL--LQDSVNPRQYQPLLERKRCESLENRIEHFVKKQRLIVKNETE 497

HSP 2 Score: 69.3218 bits (168), Expect = 1.330e-11
Identity = 40/89 (44.94%), Postives = 54/89 (60.67%), Query Frame = 1 
Query:  142 KNRKLRKYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDV-NISFCGRTDKGVSAFQQVISLVVRS 405
            K +K+RK+  D+S    RH+ LK  Y GW Y G   Q  S++ ++S L  +L ++ L E R   N   CGRTDK VSAF QVIS+ +RS
Sbjct:   84 KVQKIRKF--DWSSAHKRHVLLKITYFGWDYQGFACQEDSNDTIESNLFRALARTCLIESRATSNYHRCGRTDKEVSAFCQVISIDLRS 170
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Fly
Match: CG34140 (gene:FBgn0083976 transcript:FBtr0110975)

HSP 1 Score: 51.9878 bits (123), Expect = 2.074e-6
Identity = 44/175 (25.14%), Postives = 71/175 (40.57%), Query Frame = 2 
Query:  746 ILNKFLPED---IKILAWSPVDKDFHATFNCLGRSY---------------------------------KYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQ----FT-RNIYSVKIEP-------LDEIEPEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGK 1126
            +LN+ L +    I++L+   V   FH  ++ +GR+Y                                  YF    +  I+ +Q A    IG HDFR F + + +Q + +    FT R I  + I P        + I+       + I I   SF+Y Q+R IVG L+ +G G+
Sbjct:   91 VLNRTLNKQRLPIRVLSSKLVANSFHCRYDAIGRTYLYRFAVAKVPTLGDCSLRNRSFETFIPVEEIDRCYFLQSSSFDIKRVQAAARMFIGVHDFRTFMS-VSRQKVCRDHPMFTVRKIDEINIRPGETRALSSNAIQAAETYNYWDIEIRAKSFLYKQVRRIVGALIALGNGR 264
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Zebrafish
Match: pus3 (pseudouridylate synthase 3 [Source:ZFIN;Acc:ZDB-GENE-030131-9433])

HSP 1 Score: 174.096 bits (440), Expect = 4.090e-46
Identity = 95/261 (36.40%), Postives = 150/261 (57.47%), Query Frame = 2 
Query:  716 TNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHETKMYGIY---ISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQT-SEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRHSKKIDSALLLNYINKDVRFENWKPLLERLKESTLEARI 1486
            T+  E+ YV ILN+ LP+DI+IL W+PV+  F A F+C  R+Y+Y+FPRG+L +ELM EA  +  G+HDFRN C M V  G+ QF R I S  ++P     P      + ++   + G +F+YHQ+RC++ +LL+IG   E  E       +D      KP Y MA + PLV ++  +  ++W+  +E+  +I+N   H  W++   K  IL   +  L Q  S ++ S  L  ++ +  R + ++PLL R +  +LE+RI
Sbjct:  150 TSADELPYVKILNRVLPQDIRILQWAPVESGFSARFDCQSRTYRYYFPRGDLDLELMAEAAKRYEGTHDFRNLCKMDVGNGVLQFQRTILSASVQPAQLPHPSVHNDPHQLFVFQVKGLAFLYHQVRCMMALLLLIGQKLEAPE--VIDQLLDVEKNPRKPQYSMAVDYPLVLYDCHFEGVNWRNETEEEIHILN-TFHQHWVQNAVKTQILLGMIQGL-QNTSTEMGS--LQCWLMEGSRQKKYQPLLSRPRCESLESRI 404

HSP 2 Score: 91.6633 bits (226), Expect = 1.123e-18
Identity = 45/92 (48.91%), Postives = 62/92 (67.39%), Query Frame = 1 
Query:  136 RMKNRKLRKYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDVNI-SFCGRTDKGVSAFQQVISLVVRSN 408
            R K +++ K   DFS +  RH+AL+  YLGW+Y G   Q  +DN V+++L E+L K+KL +DR  +    CGRTDKGVSAF QVIS+ +RS 
Sbjct:   40 RQKKQRVEK-PFDFSAHPRRHVALRLAYLGWQYQGFAVQENTDNTVEARLFEALLKTKLIQDRQTSCYHRCGRTDKGVSAFSQVISIDLRST 130
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Zebrafish
Match: pus3 (pseudouridylate synthase 3 [Source:ZFIN;Acc:ZDB-GENE-030131-9433])

HSP 1 Score: 91.6633 bits (226), Expect = 1.939e-20
Identity = 47/103 (45.63%), Postives = 65/103 (63.11%), Query Frame = 1 
Query:  106 TEENLKLESERMKNRKLR-KYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDVNI-SFCGRTDKGVSAFQQVISLVVRSN 408
            TEE  +      + +K R +   DFS +  RH+AL+  YLGW+Y G   Q  +DN V+++L E+L K+KL +DR  +    CGRTDKGVSAF QVIS+ +RS 
Sbjct:   28 TEEKSQTPESGSRQKKQRVEKPFDFSAHPRRHVALRLAYLGWQYQGFAVQENTDNTVEARLFEALLKTKLIQDRQTSCYHRCGRTDKGVSAFSQVISIDLRST 130

HSP 2 Score: 53.5286 bits (127), Expect = 2.036e-7
Identity = 21/42 (50.00%), Postives = 30/42 (71.43%), Query Frame = 2 
Query:  716 TNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRS 841
            T+  E+ YV ILN+ LP+DI+IL W+PV+  F A F+C  R+
Sbjct:  150 TSADELPYVKILNRVLPQDIRILQWAPVESGFSARFDCQSRT 191
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Zebrafish
Match: pusl1 (pseudouridine synthase like 1 [Source:ZFIN;Acc:ZDB-GENE-101110-1])

HSP 1 Score: 58.9214 bits (141), Expect = 4.723e-9
Identity = 38/147 (25.85%), Postives = 61/147 (41.50%), Query Frame = 2 
Query:  767 EDIKILAWSPVDKDFHATFNCLGRSYKYFFPRG---------------------NLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEH---ETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKV 1135
            E I+I     V  DFHA +    R+Y Y    G                      L+I+ M+EA   L+G+HDF  F  +          + +   ++EP +     H   + + + +     SF+Y Q+R + GVL+ IG G+  V
Sbjct:   22 EPIRITRAYRVHSDFHARYQAQSRTYVYRLALGCQHHTLMPLTEKDLCWALRDTRLNIDAMREASALLLGTHDFSTFRALSSDAPFKCPVKTLDLAQLEPGESFSQRHFQRDIQFWELTFKSRSFLYRQVRRMTGVLVAIGQGRLSV 168
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Zebrafish
Match: pusl1 (pseudouridine synthase like 1 [Source:ZFIN;Acc:ZDB-GENE-101110-1])

HSP 1 Score: 58.151 bits (139), Expect = 2.455e-8
Identity = 38/147 (25.85%), Postives = 61/147 (41.50%), Query Frame = 2 
Query:  767 EDIKILAWSPVDKDFHATFNCLGRSYKYFFPRG---------------------NLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEH---ETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKV 1135
            E I+I     V  DFHA +    R+Y Y    G                      L+I+ M+EA   L+G+HDF  F  +          + +   ++EP +     H   + + + +     SF+Y Q+R + GVL+ IG G+  V
Sbjct:   99 EPIRITRAYRVHSDFHARYQAQSRTYVYRLALGCQHHTLMPLTEKDLCWALRDTRLNIDAMREASALLLGTHDFSTFRALSSDAPFKCPVKTLDLAQLEPGESFSQRHFQRDIQFWELTFKSRSFLYRQVRRMTGVLVAIGQGRLSV 245
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Zebrafish
Match: pusl1 (pseudouridine synthase like 1 [Source:ZFIN;Acc:ZDB-GENE-101110-1])

HSP 1 Score: 57.7658 bits (138), Expect = 2.669e-8
Identity = 38/147 (25.85%), Postives = 61/147 (41.50%), Query Frame = 2 
Query:  767 EDIKILAWSPVDKDFHATFNCLGRSYKYFFPRG---------------------NLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEH---ETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKV 1135
            E I+I     V  DFHA +    R+Y Y    G                      L+I+ M+EA   L+G+HDF  F  +          + +   ++EP +     H   + + + +     SF+Y Q+R + GVL+ IG G+  V
Sbjct:   99 EPIRITRAYRVHSDFHARYQAQSRTYVYRLALGCQHHTLMPLTEKDLCWALRDTRLNIDAMREASALLLGTHDFSTFRALSSDAPFKCPVKTLDLAQLEPGESFSQRHFQRDIQFWELTFKSRSFLYRQVRRMTGVLVAIGQGRLSV 245
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Xenopus
Match: prkd3 (protein kinase D3 [Source:Xenbase;Acc:XB-GENE-6036037])

HSP 1 Score: 175.252 bits (443), Expect = 5.795e-46
Identity = 84/214 (39.25%), Postives = 126/214 (58.88%), Query Frame = 2 
Query:  701 LFLSDTNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHET-KMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTIL 1339
            L  S   + EI YV ILN+ LP DI++L+W+ V   F A F+C  R+Y+Y FP   L +E M +A   L G+HDFRN C M V  G+  F R + S ++EP+ EI  +    ++Y + I+G +F+YHQ+RCI+GVL ++G GKEK E       +D      KP Y MA E PLV ++  ++D+ W + ++A ++   +L  LW +   K  +L
Sbjct:  131 LRCSGDGKPEIRYVHILNRVLPPDIRVLSWAAVPPSFSARFSCRSRTYRYLFPCAQLDVEAMDKAARLLEGTHDFRNLCKMDVANGVMNFIRTVLSARVEPVPEIPGDMGPFQLYRLQITGLAFLYHQVRCIMGVLFLVGQGKEKPE--VITELLDVAKNPRKPQYNMAVEFPLVLYDCHFDDVQWVSEKEAQSLTVSHLQRLWTQEAVKTQML 342

HSP 2 Score: 76.2554 bits (186), Expect = 1.734e-13
Identity = 37/88 (42.05%), Postives = 53/88 (60.23%), Query Frame = 1 
Query:  142 KNRKLRKYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDV-NISFCGRTDKGVSAFQQVISLVVR 402
            K ++  + + DFS +  +H+AL+  YLGW Y G   Q  + N V+  +  +L K++L E R   N   CGRTD+GVSA  QVISL +R
Sbjct:   45 KQKQRPQRAFDFSAHPKQHVALRLAYLGWGYQGFASQENTKNTVEETVFNALIKTRLVESRQTSNYHRCGRTDRGVSALGQVISLDLR 132
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Mouse
Match: Pus3 (pseudouridine synthase 3 [Source:MGI Symbol;Acc:MGI:1914299])

HSP 1 Score: 167.162 bits (422), Expect = 1.811e-43
Identity = 93/279 (33.33%), Postives = 138/279 (49.46%), Query Frame = 2 
Query:  710 SDTNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHET----KMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQ-----RHSKKIDSALLLNYIN-----------KDVRFENWKPLLERLKESTLEARI 1486
            +D   KEI Y  ILN+ LP DI++LAW+PV+  F A F+CL R+Y+YFFPR +L I  M  A  K +G+HDFRN C M V  G+  F R I   +++ + +   E       ++    + G +F+YHQ+RC++ +L +IG G EK E       ++      KP Y MA E PLV ++  + +  W    +       +L  LW     K  +L   L  L+          K+D A     I            + V+   +KPL++R K   LE+RI
Sbjct:  152 ADDLAKEIRYTHILNRVLPADIRVLAWAPVEPSFSARFSCLERTYRYFFPRADLDIATMNYAAQKYVGTHDFRNLCKMDVANGVINFQRTILCAQVQLVAQSPGEERRQEPFQLCQFEVIGQAFLYHQVRCMMAILFLIGQGMEKPE--IIDELLNIQKNPQKPQYSMAVEFPLVLYDCKFENTKWIYDHEVQEFNVTHLQQLWANHAVKTHMLYSMLQGLDSVMVTCAAGTKMDEATEWRNIQPPVIKHTSAFVEGVKMRTYKPLMDRPKCQGLESRI 428

HSP 2 Score: 87.8113 bits (216), Expect = 2.684e-17
Identity = 44/83 (53.01%), Postives = 54/83 (65.06%), Query Frame = 1 
Query:  160 KYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDV-NISFCGRTDKGVSAFQQVISLVVRS 405
            K + DFS +  RH+ALK  YLGW Y G   Q  + N ++ KL E+L K++L E R   N   CGRTDKGVSAF QVISL +RS
Sbjct:   53 KRAFDFSAHGRRHVALKIAYLGWGYQGFASQENTSNTIEEKLFEALTKTRLVESRQTSNYHRCGRTDKGVSAFGQVISLDLRS 135
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Mouse
Match: Pus3 (pseudouridine synthase 3 [Source:MGI Symbol;Acc:MGI:1914299])

HSP 1 Score: 140.584 bits (353), Expect = 5.256e-36
Identity = 62/147 (42.18%), Postives = 90/147 (61.22%), Query Frame = 2 
Query:  710 SDTNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHET----KMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVE 1138
            +D   KEI Y  ILN+ LP DI++LAW+PV+  F A F+CL R+Y+YFFPR +L I  M  A  K +G+HDFRN C M V  G+  F R I   +++ + +   E       ++    + G +F+YHQ+RC++ +L +IG G EK E
Sbjct:  152 ADDLAKEIRYTHILNRVLPADIRVLAWAPVEPSFSARFSCLERTYRYFFPRADLDIATMNYAAQKYVGTHDFRNLCKMDVANGVINFQRTILCAQVQLVAQSPGEERRQEPFQLCQFEVIGQAFLYHQVRCMMAILFLIGQGMEKPE 298

HSP 2 Score: 87.8113 bits (216), Expect = 4.486e-18
Identity = 44/83 (53.01%), Postives = 54/83 (65.06%), Query Frame = 1 
Query:  160 KYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDV-NISFCGRTDKGVSAFQQVISLVVRS 405
            K + DFS +  RH+ALK  YLGW Y G   Q  + N ++ KL E+L K++L E R   N   CGRTDKGVSAF QVISL +RS
Sbjct:   53 KRAFDFSAHGRRHVALKIAYLGWGYQGFASQENTSNTIEEKLFEALTKTRLVESRQTSNYHRCGRTDKGVSAFGQVISLDLRS 135
[Close]
BLAST of tRNA pseudouridine synthase vs. UniProt/SwissProt
Match: sp|Q9JI38|PUS3_MOUSE (tRNA pseudouridine(38/39) synthase OS=Mus musculus OX=10090 GN=Pus3 PE=1 SV=2)

HSP 1 Score: 167.162 bits (422), Expect = 1.267e-42
Identity = 93/279 (33.33%), Postives = 138/279 (49.46%), Query Frame = 2 
Query:  710 SDTNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHET----KMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQ-----RHSKKIDSALLLNYIN-----------KDVRFENWKPLLERLKESTLEARI 1486
            +D   KEI Y  ILN+ LP DI++LAW+PV+  F A F+CL R+Y+YFFPR +L I  M  A  K +G+HDFRN C M V  G+  F R I   +++ + +   E       ++    + G +F+YHQ+RC++ +L +IG G EK E       ++      KP Y MA E PLV ++  + +  W    +       +L  LW     K  +L   L  L+          K+D A     I            + V+   +KPL++R K   LE+RI
Sbjct:  152 ADDLAKEIRYTHILNRVLPADIRVLAWAPVEPSFSARFSCLERTYRYFFPRADLDIATMNYAAQKYVGTHDFRNLCKMDVANGVINFQRTILCAQVQLVAQSPGEERRQEPFQLCQFEVIGQAFLYHQVRCMMAILFLIGQGMEKPE--IIDELLNIQKNPQKPQYSMAVEFPLVLYDCKFENTKWIYDHEVQEFNVTHLQQLWANHAVKTHMLYSMLQGLDSVMVTCAAGTKMDEATEWRNIQPPVIKHTSAFVEGVKMRTYKPLMDRPKCQGLESRI 428

HSP 2 Score: 87.8113 bits (216), Expect = 1.877e-16
Identity = 44/83 (53.01%), Postives = 54/83 (65.06%), Query Frame = 1 
Query:  160 KYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDV-NISFCGRTDKGVSAFQQVISLVVRS 405
            K + DFS +  RH+ALK  YLGW Y G   Q  + N ++ KL E+L K++L E R   N   CGRTDKGVSAF QVISL +RS
Sbjct:   53 KRAFDFSAHGRRHVALKIAYLGWGYQGFASQENTSNTIEEKLFEALTKTRLVESRQTSNYHRCGRTDKGVSAFGQVISLDLRS 135
[Close]
BLAST of tRNA pseudouridine synthase vs. UniProt/SwissProt
Match: sp|Q3SX07|PUS3_BOVIN (tRNA pseudouridine(38/39) synthase OS=Bos taurus OX=9913 GN=PUS3 PE=2 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 1.895e-42
Identity = 93/273 (34.07%), Postives = 138/273 (50.55%), Query Frame = 2 
Query:  728 EIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEP----LDEIEPEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQ-----RHSKKIDSALLLNYIN-----------KDVRFENWKPLLERLKESTLEARI 1486
            EI Y  ILN+ LP DI++LAW+PV+  F A F+CL R+Y+YFFPR NL I  M  A  K +G+HDFRN C M V  G+  F R I S +++     L E   +   ++    ++G +F+YHQ+RC++ VL +IG G EK E       ++      KP Y MA E PLV ++  + ++ W    +       +L  LW     K  +L   L  L+          K+D  +    +            + V+   +KPL++R K   LE+RI
Sbjct:  157 EIRYTHILNRVLPPDIRVLAWAPVETSFSARFSCLERTYRYFFPRANLDIVTMNYAAQKYVGTHDFRNLCKMDVANGVINFQRTILSAQVQRVGQNLGEEGWQEPFQLCQFEVTGQAFLYHQVRCMMAVLFLIGQGMEKPE--VIDELLNIEKNPQKPQYSMAVEFPLVLYDCKFENIKWIYDREVQEFNVTHLQQLWANHAVKTQMLYSMLQGLDSVALPCGTGPKMDGMIEWRNVKPSVTKQTSAFVEGVKMRTYKPLMDRPKCQGLESRI 427

HSP 2 Score: 89.7373 bits (221), Expect = 4.513e-17
Identity = 44/85 (51.76%), Postives = 57/85 (67.06%), Query Frame = 1 
Query:  160 KYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDV-NISFCGRTDKGVSAFQQVISLVVRSNL 411
            K + DFS +  RH+ALK  YLGW Y G   Q  + N ++ KL E+L K++L E+R   N   CGRTDKGVSAF QVISL +RS++
Sbjct:   52 KRAFDFSAHGQRHVALKIAYLGWGYQGFASQENTSNTIEEKLFEALTKTRLVENRQTSNYHRCGRTDKGVSAFGQVISLDLRSHI 136
[Close]
BLAST of tRNA pseudouridine synthase vs. UniProt/SwissProt
Match: sp|Q9BZE2|PUS3_HUMAN (tRNA pseudouridine(38/39) synthase OS=Homo sapiens OX=9606 GN=PUS3 PE=1 SV=3)

HSP 1 Score: 163.696 bits (413), Expect = 1.825e-41
Identity = 91/272 (33.46%), Postives = 138/272 (50.74%), Query Frame = 2 
Query:  731 IDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPE----HETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRH-----SKKIDSALLLNYIN-----------KDVRFENWKPLLERLKESTLEARI 1486
            I Y  ILN+ LP DI+ILAW+PV+  F A F+CL R+Y+YFFPR +L I  M  A  K +G+HDFRN C M V  G+  F R I S +++ + +   E       ++    ++G +F+YHQ+RC++ +L +IG G EK E       ++      KP Y MA E PLV ++  + ++ W   ++A      +L  LW     K  +L   L  L+          K+D       +            + V+   +KPL++R K   LE+RI
Sbjct:  158 IRYTHILNRVLPPDIRILAWAPVEPSFSARFSCLERTYRYFFPRADLDIVTMDYAAQKYVGTHDFRNLCKMDVANGVINFQRTILSAQVQLVGQSPGEGRWQEPFQLCQFEVTGQAFLYHQVRCMMAILFLIGQGMEKPE--IIDELLNIEKNPQKPQYSMAVEFPLVLYDCKFENVKWIYDQEAQEFNITHLQQLWANHAVKTHMLYSMLQGLDTVPVPCGIGPKMDGMTEWGNVKPSVIKQTSAFVEGVKMRTYKPLMDRPKCQGLESRI 427

HSP 2 Score: 86.6557 bits (213), Expect = 3.946e-16
Identity = 42/83 (50.60%), Postives = 55/83 (66.27%), Query Frame = 1 
Query:  160 KYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDV-NISFCGRTDKGVSAFQQVISLVVRS 405
            K + DFS +  RH+AL+  Y+GW Y G   Q  ++N ++ KL E+L K++L E R   N   CGRTDKGVSAF QVISL +RS
Sbjct:   52 KRAFDFSAHGRRHVALRIAYMGWGYQGFASQENTNNTIEEKLFEALTKTRLVESRQTSNYHRCGRTDKGVSAFGQVISLDLRS 134
[Close]
BLAST of tRNA pseudouridine synthase vs. UniProt/SwissProt
Match: sp|P31115|PUS3_YEAST (tRNA pseudouridine(38/39) synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DEG1 PE=1 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 1.562e-29
Identity = 69/181 (38.12%), Postives = 103/181 (56.91%), Query Frame = 2 
Query:  719 NEKEIDYVFILNKFLPEDIKILAWS-PVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQ 1258
            + +EI YV +LN+ LP+DI+I A       +F A F+C+ R YKY F   NL+IE M +A +  +G  DFRNFC +   + IT F R I S KI PL E         Y   + GS+F++HQ+RC++ +L ++G   E  E    L   D      +P Y+MA ++PL+ ++  + +MDWQ
Sbjct:  180 DSREIPYVHVLNQLLPDDIRISAVCLRPPPNFDARFSCVHRHYKYIFNGKNLNIEKMSKAASYFVGERDFRNFCKLDGSKQITNFKRTIISSKILPLSET-------FYCFDLVGSAFLWHQVRCMMAILFLVGQSLEVPE--IVLRLTDIEKTPQRPVYEMANDIPLLLYDCKFPEMDWQ 351

HSP 2 Score: 79.337 bits (194), Expect = 7.823e-14
Identity = 48/101 (47.52%), Postives = 59/101 (58.42%), Query Frame = 1 
Query:  127 ESERMKNRKLRKYSLDFSEYKLRHIALKFCYLGWRYSGVIGQ--YQSDNVVKSKLQESLFKSKLTED---RDVNISFCGRTDKGVSAFQQVISLVVRSNLV 414
            E  R K +K  K   DFS++  R IAL+F YLGW Y+G+  Q  Y     V+  + E++ K KL      +D   S CGRTDKGVSA  QVISL VRSNL 
Sbjct:   71 EVTRSKAKKAPK-KFDFSKHNTRFIALRFAYLGWNYNGLAVQKEYTPLPTVEGTILEAMNKCKLVPSMVLQDYKFSRCGRTDKGVSAMNQVISLEVRSNLT 170
[Close]
BLAST of tRNA pseudouridine synthase vs. UniProt/SwissProt
Match: sp|Q09524|TG124_CAEEL (Probable tRNA pseudouridine synthase tag-124 OS=Caenorhabditis elegans OX=6239 GN=tag-124 PE=3 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 1.239e-28
Identity = 69/217 (31.80%), Postives = 116/217 (53.46%), Query Frame = 2 
Query:  725 KEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCT--MLVKQGITQFTRNIYSVKIEPLDEIEPEHET-KMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYN--DMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSEL 1360
            +E+ YV +LN  LP+ I++ AW+PV + F+A F+C  R+YKY F + +L++E M++    L+G HDF NFC   M  K+ +  + R +Y VK+E +    PE++   M  + +SGS F++H IR IV +L  I  G+E  +       +D      +P Y +A + PL   +  Y   D++W+  +         L   W  +  +  +++  L EL
Sbjct:  128 EELPYVKMLNGVLPKTIRVFAWAPVAQTFNARFDCNRRTYKYSFAKADLNLEKMRQGAELLVGEHDFSNFCQIDMNEKRLLQSYVRKVYEVKVEQV-STHPENDMYSMVELTVSGSGFLWHMIRYIVTILQEI--GRENEQPSLISQLLDLKKYPSRPQYTLASDTPLCLFDCGYKSEDVEWKVHDYTLKSTVTGLQKTWATYQARSRMMENMLGEL 341

HSP 2 Score: 80.8777 bits (198), Expect = 1.997e-14
Identity = 37/81 (45.68%), Postives = 55/81 (67.90%), Query Frame = 1 
Query:  166 SLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTED-RDVNISFCGRTDKGVSAFQQVISLVVRS 405
            +LDF  +  R IA++F YLGW + G++ Q  + N V++ + ++L K+ L ED    + S CGRTDKGVSAF+Q  ++VVRS
Sbjct:   21 TLDFLAHPRRKIAIQFFYLGWEHDGLVQQPHTQNTVENHIMQALIKTHLIEDWTKCDFSRCGRTDKGVSAFKQTAAMVVRS 101
[Close]
BLAST of tRNA pseudouridine synthase vs. TrEMBL
Match: A0A4Y2QSA2 (tRNA pseudouridine(38/39) synthase OS=Araneus ventricosus OX=182803 GN=PUS3 PE=3 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 5.393e-50
Identity = 107/263 (40.68%), Postives = 154/263 (58.56%), Query Frame = 2 
Query:  716 TNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRHSKKIDSALL--LNYINKDVRFENWKPLLERLKESTLEARIDKHN 1498
            +++KEIDY  ILN+ LP+DIKI+AWSPV+  F A F+C  R+YKY+FP GNL I+ MQE G KL+G HD+RN C M V  G+  + R I+SV I+ L         ++  + ++G +F++HQIRCIV +L +IG GKE  E       +D      KP Y +A E+PLV  +  Y D+DW  +E++   +   L ++W     K TI++  LSEL    S   D  +L     +   VR   +K LLER    +LE RI+ ++
Sbjct:  148 SSDKEIDYPSILNRVLPDDIKIIAWSPVETTFSARFDCKRRTYKYWFPIGNLDIQRMQEGGLKLVGEHDYRNICKMDVGNGVVNYMRKIFSVDIKEL-TTSDSRSYQLAELTVNGQAFLWHQIRCIVSLLFLIGQGKE--ECSVIDQLLDIENCPRKPQYDIASEIPLVLFDCSYEDVDWIYNEESLKFVIKQLQNMWTHHGIKTTIIRSMLSELESLGSS--DDVILNQAECLIPGVRPRAYKRLLERPCCESLEERIEHYS 405

HSP 2 Score: 91.2781 bits (225), Expect = 2.233e-15
Identity = 46/82 (56.10%), Postives = 57/82 (69.51%), Query Frame = 1 
Query:  172 DFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDV-NISFCGRTDKGVSAFQQVISLVVRSNLV 414
            DFS YK RH+ALKF YLGW Y G   Q  ++  ++ +L  +L K+KL E R+  N   CGRTDKGVSAF QVISL +RSNL+
Sbjct:   49 DFSRYKKRHVALKFLYLGWDYCGFAVQTHTEKTIEEQLFNALMKTKLLESRETSNYHRCGRTDKGVSAFSQVISLDLRSNLL 130
[Close]
BLAST of tRNA pseudouridine synthase vs. TrEMBL
Match: D2A375 (tRNA pseudouridine(38/39) synthase-like Protein OS=Tribolium castaneum OX=7070 GN=TcasGA2_TC030541 PE=3 SV=2)

HSP 1 Score: 190.274 bits (482), Expect = 5.547e-49
Identity = 100/259 (38.61%), Postives = 157/259 (60.62%), Query Frame = 2 
Query:  710 SDTNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRHSKKIDSALLLNYINKDVRFENWKPLLERLKESTLEARI 1486
             D  E EI+Y  ILN+ LPE+I+ +AW PV+  F A F+C  R+YKYFFP+ NL I+ M+ A   L+G+HDFRNFC M V  G+ +F RNI SV I    E E      +  + I G++F++HQIRCIVG+L+++G GKEK E    L  +D      KP Y MA E+PL  ++  Y++++W   ++A NI+   L+  W+    K  ++K  L +L +  S+    +  ++++ + V+ +N+ P+++R    +LE +I
Sbjct:  129 GDDFESEINYCKILNRVLPENIQFVAWCPVESAFSARFDCKNRTYKYFFPKSNLDIDAMKIAANYLVGTHDFRNFCKMDVNNGVVEFKRNILSVDISLFQEDE---RYSIQVLTIKGNAFLWHQIRCIVGILILVGQGKEKPE--VVLDLLDITRNPRKPDYHMASEIPLNLYDCHYDNVNWIYDKEALNIVKAKLYKFWMFSSIKTAMMKSMLVDLEKEASELDHDS--IDFLIQGVKRKNYVPVMKRQLCESLEDKI 380

HSP 2 Score: 80.8777 bits (198), Expect = 4.470e-12
Identity = 43/98 (43.88%), Postives = 58/98 (59.18%), Query Frame = 1 
Query:  118 LKLESERMKNRKLRKYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDR-DVNISFCGRTDKGVSAFQQVISLVVRSN 408
            LK  + +  N+   +   DF   K RH+ LKF YLGW Y G + Q  + N ++  +  +L K+ L E+R   N   CGRTDKGVS+F QVISL +RSN
Sbjct:   31 LKGTATKPANQPKCQKPFDFQNCKFRHVLLKFLYLGWDYQGYVTQEDTVNTIEHHVFNALIKTCLIENRSSANYHRCGRTDKGVSSFSQVISLDLRSN 128
[Close]
BLAST of tRNA pseudouridine synthase vs. TrEMBL
Match: A0A0R3UJN5 (tRNA pseudouridine synthase OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS7736 PE=3 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 4.552e-48
Identity = 105/255 (41.18%), Postives = 141/255 (55.29%), Query Frame = 2 
Query:  728 EIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRHSKKIDSALLLNYINKDVRFENWKPLLERLKESTLEARIDK 1492
            E DYVFI N+ LP +I+ILAW PV  +F    +C  RSYKYFFP  +L+I++M++A  KL G HDFRNFCT  V  G+    R IYSV I   DE + +   +   + I GSSF+YHQIRCIV +L+ IG G E  E       +D      KP YQMA   PL+F E  + +++WQTS  A   I   L +LW K   +  + +  L  + Q        A  + +    +  E  KP+L+R  E TLE R+ K
Sbjct:   86 EYDYVFIGNRNLPPEIRILAWCPVPLEFSPRHDCTSRSYKYFFPAADLNIDIMRKAAAKLEGQHDFRNFCTSQVANGVVNHERVIYSVTISDKDEDKTDPR-RFCCLDIRGSSFLYHQIRCIVSLLVTIGRGLEPSE--LIDCLLDVQQTPAKPQYQMAEPEPLLFFEPEFQNLEWQTSPAAQEDILRTLQTLWTKLSVRAKVAETMLKTVEQCSPNNYTWA--ITHEAPSIVREKRKPILKRSTEETLEERVKK 335
[Close]
BLAST of tRNA pseudouridine synthase vs. TrEMBL
Match: A0A087U2S7 (tRNA pseudouridine(38/39) synthase (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_08477 PE=3 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 1.533e-47
Identity = 101/275 (36.73%), Postives = 158/275 (57.45%), Query Frame = 2 
Query:  710 SDTNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHETKMYGI---YISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRHSKKIDSALL--LNYINKDVRFENWKPLLERLKESTLEARIDKHNILMKVEI 1519
            S  ++KEIDY  +LN+ LP +I+++ W+PVD  F A F+C  R+YKY+ P G+L I +M+EA  KL+G HDFRNFC M V  G+  + R I+SV I+P        E K Y I    + G +F++HQIRCI+ VL +IG GKE  +       +D      KP Y MA E+PLV  +  Y++++W  ++++  ++  +L S+W     K  I +  L EL +     +D+ +L  +  +   V+   +K LL+R    +LE RI+ +N  +K  +
Sbjct:  148 SKVSDKEIDYPSVLNRVLPPEIRVIGWAPVDTTFSARFDCKQRTYKYWLPLGDLDISIMREAAQKLVGEHDFRNFCKMDVGNGVINYKRRIFSVDIQPFSSC----EVKAYDIIELTVVGQAFLWHQIRCIISVLFLIGQGKEDCQ--IVDQLLDIESCPRKPQYNMASEIPLVLFDCAYDNIEWIYNKESLKLVIKHLQSIWTYNAVKTVITRKMLQELEKMCP--VDNPVLNQVECLLPGVKPREYKCLLDRQCCESLEERIEHYNKKIKTSL 414

HSP 2 Score: 94.3597 bits (233), Expect = 2.359e-16
Identity = 57/118 (48.31%), Postives = 76/118 (64.41%), Query Frame = 1 
Query:   91 ISYLSTEENLKL-------ESERMKNRKLRKYS---LDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDV-NISFCGRTDKGVSAFQQVISLVVRSNL 411
            +  LS EE +K+       ES + KN+ +RK S    DF +YK RH+ALKF YLGW YSG   Q  ++  ++++L  +L K+KL E R+  N   CGRTDKGVSAF QVISL +R+NL
Sbjct:   15 LEALSKEELIKIIKHLQDKESPK-KNKDIRKKSSTPFDFKKYKKRHVALKFLYLGWEYSGFTVQIHTEKTIEAQLFNALEKTKLLESRETSNYHRCGRTDKGVSAFCQVISLDLRTNL 131
[Close]
BLAST of tRNA pseudouridine synthase vs. TrEMBL
Match: A0A5K3F2C8 (tRNA pseudouridine synthase OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 2.313e-47
Identity = 105/255 (41.18%), Postives = 141/255 (55.29%), Query Frame = 2 
Query:  728 EIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRHSKKIDSALLLNYINKDVRFENWKPLLERLKESTLEARIDK 1492
            E DYVFI N+ LP +I+ILAW PV  +F    +C  RSYKYFFP  +L+I++M++A  KL G HDFRNFCT  V  G+    R IYSV I   DE + +   +   + I GSSF+YHQIRCIV +L+ IG G E  E       +D      KP YQMA   PL+F E  + +++WQTS  A   I   L +LW K   +  + +  L  + Q        A  + +    +  E  KP+L+R  E TLE R+ K
Sbjct:  161 EYDYVFIGNRNLPPEIRILAWCPVPLEFSPRHDCTSRSYKYFFPAADLNIDIMRKAAAKLEGQHDFRNFCTSQVANGVVNHERVIYSVTISDKDEDKTDPR-RFCCLDIRGSSFLYHQIRCIVSLLVTIGRGLEPSE--LIDCLLDVQQTPAKPQYQMAEPEPLLFFEPEFQNLEWQTSPAAQEDILRTLQTLWTKLSVRAKVAETMLKTVEQCSPNNYTWA--ITHEAPSIVREKRKPILKRSTEETLEERVKK 410

HSP 2 Score: 71.2478 bits (173), Expect = 7.161e-9
Identity = 33/81 (40.74%), Postives = 51/81 (62.96%), Query Frame = 1 
Query:  172 DFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRD-VNISFCGRTDKGVSAFQQVISLVVRSNL 411
            DFS++  R IALK  Y+GW YSG+  Q  + + +  KL ++  + ++ E+      + CGRTDKGVS   QV++L +RSN+
Sbjct:   60 DFSKFAKRRIALKILYMGWDYSGLARQEVNKSTIAEKLLDAFTRCRMLENSSTAGFAVCGRTDKGVSGLSQVVTLTLRSNV 140
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Cavefish
Match: pus3 (pseudouridine synthase 3 [Source:NCBI gene;Acc:103046008])

HSP 1 Score: 184.496 bits (467), Expect = 1.710e-49
Identity = 94/262 (35.88%), Postives = 149/262 (56.87%), Query Frame = 2 
Query:  710 SDTNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIE--PEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDW-QTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRHSKKIDSALLLNYINKDVRFENWKPLLERLKESTLEARI 1486
            S+  ++E+ YV ILN+ LP DI+ L WSP +  F A F+C+ R+Y+Y+FPRG L +ELM  A  +  G+HD+RN C M V  G+ QF R I S  ++P    E  P +  ++Y   + G +F+YHQ+RC++ VLL+IG   E  E       +D      KP Y MA + PLV HE  +  + W +  E+ S++++  L+  W +   K  +L   + +L    +    S  +  ++ +  R +N++PLLER +  +LE+RI
Sbjct:  165 SNAPKEELPYVKILNRVLPPDIRALCWSPAETGFSARFDCVYRTYRYYFPRGELDVELMATAARRYEGTHDYRNLCKMDVGNGVLQFQRTIQSASVQPAQPCEPCPVNPLQLYVFEVKGLAFLYHQVRCMMAVLLLIGQKLEAPE--IIDKLLDVENNPRKPQYSMAVDYPLVLHECHFEGLKWNKEPEEESHVLSC-LYQQWTQTAVKAQVLNSMMQDLQNTATDPQASPSVDCWLMEGSRQKNYRPLLERPRCESLESRI 423

HSP 2 Score: 86.2705 bits (212), Expect = 6.620e-17
Identity = 43/91 (47.25%), Postives = 60/91 (65.93%), Query Frame = 1 
Query:  136 RMKNRKLRKYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRD-VNISFCGRTDKGVSAFQQVISLVVRS 405
            R K +K  +   DFS +  RH+A++  YLGW Y G   Q  +DN V+++L E+L K++L +DR   N   CGRTDKGVSA+ QVIS+ +RS
Sbjct:   58 RRKGKKAERV-FDFSVHPRRHVAIRLAYLGWNYQGFAVQENTDNTVEARLFEALLKTRLIQDRQSSNYHRCGRTDKGVSAYSQVISIDLRS 147
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Cavefish
Match: pus3 (pseudouridine synthase 3 [Source:NCBI gene;Acc:103046008])

HSP 1 Score: 184.111 bits (466), Expect = 6.141e-49
Identity = 94/262 (35.88%), Postives = 149/262 (56.87%), Query Frame = 2 
Query:  710 SDTNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIE--PEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDW-QTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRHSKKIDSALLLNYINKDVRFENWKPLLERLKESTLEARI 1486
            S+  ++E+ YV ILN+ LP DI+ L WSP +  F A F+C+ R+Y+Y+FPRG L +ELM  A  +  G+HD+RN C M V  G+ QF R I S  ++P    E  P +  ++Y   + G +F+YHQ+RC++ VLL+IG   E  E       +D      KP Y MA + PLV HE  +  + W +  E+ S++++  L+  W +   K  +L   + +L    +    S  +  ++ +  R +N++PLLER +  +LE+RI
Sbjct:  220 SNAPKEELPYVKILNRVLPPDIRALCWSPAETGFSARFDCVYRTYRYYFPRGELDVELMATAARRYEGTHDYRNLCKMDVGNGVLQFQRTIQSASVQPAQPCEPCPVNPLQLYVFEVKGLAFLYHQVRCMMAVLLLIGQKLEAPE--IIDKLLDVENNPRKPQYSMAVDYPLVLHECHFEGLKWNKEPEEESHVLSC-LYQQWTQTAVKAQVLNSMMQDLQNTATDPQASPSVDCWLMEGSRQKNYRPLLERPRCESLESRI 478

HSP 2 Score: 85.8853 bits (211), Expect = 1.014e-16
Identity = 43/91 (47.25%), Postives = 60/91 (65.93%), Query Frame = 1 
Query:  136 RMKNRKLRKYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRD-VNISFCGRTDKGVSAFQQVISLVVRS 405
            R K +K  +   DFS +  RH+A++  YLGW Y G   Q  +DN V+++L E+L K++L +DR   N   CGRTDKGVSA+ QVIS+ +RS
Sbjct:  113 RRKGKKAERV-FDFSVHPRRHVAIRLAYLGWNYQGFAVQENTDNTVEARLFEALLKTRLIQDRQSSNYHRCGRTDKGVSAYSQVISIDLRS 202
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Yeast
Match: DEG1 (tRNA:pseudouridine synthase; introduces pseudouridines at position 38 or 39 in tRNA; also responsible for pseudouracil modification of some mRNAs; important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability [Source:SGD;Acc:S000001895])

HSP 1 Score: 127.102 bits (318), Expect = 2.250e-31
Identity = 69/181 (38.12%), Postives = 103/181 (56.91%), Query Frame = 2 
Query:  719 NEKEIDYVFILNKFLPEDIKILAWS-PVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQ 1258
            + +EI YV +LN+ LP+DI+I A       +F A F+C+ R YKY F   NL+IE M +A +  +G  DFRNFC +   + IT F R I S KI PL E         Y   + GS+F++HQ+RC++ +L ++G   E  E    L   D      +P Y+MA ++PL+ ++  + +MDWQ
Sbjct:  180 DSREIPYVHVLNQLLPDDIRISAVCLRPPPNFDARFSCVHRHYKYIFNGKNLNIEKMSKAASYFVGERDFRNFCKLDGSKQITNFKRTIISSKILPLSET-------FYCFDLVGSAFLWHQVRCMMAILFLVGQSLEVPE--IVLRLTDIEKTPQRPVYEMANDIPLLLYDCKFPEMDWQ 351

HSP 2 Score: 79.337 bits (194), Expect = 1.127e-15
Identity = 48/101 (47.52%), Postives = 59/101 (58.42%), Query Frame = 1 
Query:  127 ESERMKNRKLRKYSLDFSEYKLRHIALKFCYLGWRYSGVIGQ--YQSDNVVKSKLQESLFKSKLTED---RDVNISFCGRTDKGVSAFQQVISLVVRSNLV 414
            E  R K +K  K   DFS++  R IAL+F YLGW Y+G+  Q  Y     V+  + E++ K KL      +D   S CGRTDKGVSA  QVISL VRSNL 
Sbjct:   71 EVTRSKAKKAPK-KFDFSKHNTRFIALRFAYLGWNYNGLAVQKEYTPLPTVEGTILEAMNKCKLVPSMVLQDYKFSRCGRTDKGVSAMNQVISLEVRSNLT 170
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Nematostella
Match: EDO36025 (tRNA pseudouridine synthase [Source:UniProtKB/TrEMBL;Acc:A7SJV8])

HSP 1 Score: 179.104 bits (453), Expect = 2.705e-48
Identity = 102/251 (40.64%), Postives = 142/251 (56.57%), Query Frame = 2 
Query:  725 KEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLD----EIEPEHETKMYG-----IYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRHSKKIDSALLLNYINKDVRFENWKPLL 1450
            KEI YV ILNK LP++I++LAW+PV+ DF A F+C  R YKY FP  NL I+LM  A  KL G HDFRNFC M V  G+ QFTRNI S K++ +         E++ K  G       I GS+F++HQ+RC++ +LLM+G G E+ E       +D      +P Y MA ELPLV ++ +Y  + W    Q    +  NL  LW  + TK T+ +  L+ L+   S +I    L N+        ++KP+L
Sbjct:  177 KEILYVDILNKVLPKEIRVLAWAPVELDFDARFSCSYRKYKYLFPSANLDIDLMNLASKKLEGEHDFRNFCKMDVANGVIQFTRNITSFKVQKVSNATYSAPGENKAKQRGYEMCEAVICGSAFLWHQVRCMMSILLMVGQGLEQPE--VIDWLLDIEKCPQRPQYNMASELPLVLYDCVYEGITWIYDPQVLESVTKNLQQLWNTYATKATLAQELLASLD---SAEIPGNPLTNW-------SSFKPML 415

HSP 2 Score: 82.4185 bits (202), Expect = 3.836e-16
Identity = 45/94 (47.87%), Postives = 62/94 (65.96%), Query Frame = 1 
Query:  133 ERMKNRKLRKYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRD-VNISFCGRTDKGVSAFQQVISLVVRSNL 411
            +R K++  +++  DFS Y  R IAL+  YLGW   G   Q + DN V++ L ++L K+ L + R+  + S CGRTDKGVSAF QVISL VR+NL
Sbjct:   62 KRAKSKPRKEF--DFSRYNTRRIALRLSYLGWDMHGFASQEKIDNTVEAHLFDALIKACLVKSRESCSYSRCGRTDKGVSAFGQVISLNVRTNL 153
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Medaka
Match: pus3 (pseudouridine synthase 3 [Source:NCBI gene;Acc:101173982])

HSP 1 Score: 170.629 bits (431), Expect = 6.291e-45
Identity = 89/262 (33.97%), Postives = 142/262 (54.20%), Query Frame = 2 
Query:  710 SDTNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPL--DEIEPEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRHSKKIDSALLLNYINKDVRFENWKPLLERLKESTLEARID 1489
            S     E+ YV +LN+ LP+DI+IL  +PV + F A F+C  R+Y+Y+FPRG+L + LM++A  +  G+HDFRN C M V  G+ QF R I S  I P+   +     +  ++   + G +F+YHQ+RC++ VLL+IG   E  +       +D      KP Y MA + PLV H   +  + WQ   +  N +  +L   W++   K  +L+  + +L  R     +   L+    +  R + ++PLLER    +LE+RI+
Sbjct:  167 SKAASSELPYVKMLNRVLPQDIRILDCAPVAEGFSARFDCQSRTYRYYFPRGSLDVALMEDAAKRYEGTHDFRNLCKMDVGNGVLQFERTILSATIRPMWSQQASSMDQYSLFVFEVKGLAFLYHQVRCMMAVLLLIGQKLE--DPQIISQLLDVENNPRKPQYSMAVDYPLVLHHCHFEGLSWQQDTEELNYVMSSLQQHWIQSAVKAQVLQEMIRDLKDRGGVSSNHCWLV----EGSRQKKYRPLLERPCCESLESRIN 422

HSP 2 Score: 89.3521 bits (220), Expect = 5.546e-18
Identity = 45/93 (48.39%), Postives = 62/93 (66.67%), Query Frame = 1 
Query:  133 ERMKNRKLRKYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRD-VNISFCGRTDKGVSAFQQVISLVVRSN 408
            ER K+ K R +  DF+ +  RH+AL+  YLGW Y G   Q  +DN V+++L E+L K++L +DR   N   CGRTDKGVSAF QVI++ +RS 
Sbjct:   57 ERKKSGKERPF--DFAAHPRRHVALRLAYLGWAYQGFAVQENTDNTVEARLFEALLKTRLIQDRQSSNYHRCGRTDKGVSAFSQVITIDLRST 147
[Close]
BLAST of tRNA pseudouridine synthase vs. Ensembl Medaka
Match: pus3 (pseudouridine synthase 3 [Source:NCBI gene;Acc:101173982])

HSP 1 Score: 170.629 bits (431), Expect = 6.291e-45
Identity = 89/262 (33.97%), Postives = 142/262 (54.20%), Query Frame = 2 
Query:  710 SDTNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPL--DEIEPEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRHSKKIDSALLLNYINKDVRFENWKPLLERLKESTLEARID 1489
            S     E+ YV +LN+ LP+DI+IL  +PV + F A F+C  R+Y+Y+FPRG+L + LM++A  +  G+HDFRN C M V  G+ QF R I S  I P+   +     +  ++   + G +F+YHQ+RC++ VLL+IG   E  +       +D      KP Y MA + PLV H   +  + WQ   +  N +  +L   W++   K  +L+  + +L  R     +   L+    +  R + ++PLLER    +LE+RI+
Sbjct:  167 SKAASSELPYVKMLNRVLPQDIRILDCAPVAEGFSARFDCQSRTYRYYFPRGSLDVALMEDAAKRYEGTHDFRNLCKMDVGNGVLQFERTILSATIRPMWSQQASSMDQYSLFVFEVKGLAFLYHQVRCMMAVLLLIGQKLE--DPQIISQLLDVENNPRKPQYSMAVDYPLVLHHCHFEGLSWQQDTEELNYVMSSLQQHWIQSAVKAQVLQEMIRDLKDRGGVSSNHCWLV----EGSRQKKYRPLLERPCCESLESRIN 422

HSP 2 Score: 89.3521 bits (220), Expect = 5.546e-18
Identity = 45/93 (48.39%), Postives = 62/93 (66.67%), Query Frame = 1 
Query:  133 ERMKNRKLRKYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRD-VNISFCGRTDKGVSAFQQVISLVVRSN 408
            ER K+ K R +  DF+ +  RH+AL+  YLGW Y G   Q  +DN V+++L E+L K++L +DR   N   CGRTDKGVSAF QVI++ +RS 
Sbjct:   57 ERKKSGKERPF--DFAAHPRRHVALRLAYLGWAYQGFAVQENTDNTVEARLFEALLKTRLIQDRQSSNYHRCGRTDKGVSAFSQVITIDLRST 147
[Close]
BLAST of tRNA pseudouridine synthase vs. Planmine SMEST
Match: SMESG000041371.1 (SMESG000041371.1)

HSP 1 Score: 522.316 bits (1344), Expect = 4.183e-174
Identity = 268/270 (99.26%), Postives = 270/270 (100.00%), Query Frame = 2 
Query:  713 DTNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRHSKKIDSALLLNYINKDVRFENWKPLLERLKESTLEARIDKHNILMKVEID 1522
            +TNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEIIDQLLDIDGIGGLGKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQ+HSKKIDSALLLNYINKDVRFENWKPLLERLKESTLEARIDKHNILMKVEID
Sbjct:  147 NTNEKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEIIDQLLDIDGIGGLGKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQKHSKKIDSALLLNYINKDVRFENWKPLLERLKESTLEARIDKHNILMKVEID 416

HSP 2 Score: 261.536 bits (667), Expect = 6.199e-77
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 1 
Query:   28 MKNFISSLYIFSKLSFKRCGPISYLSTEENLKLESERMKNRKLRKYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDVNISFCGRTDKGVSAFQQVISLVVRSNLV 414
            MKNFISSLYIFSKLSFKRCGPISYLSTEENLKLESERMKNRKLRKYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDVNISFCGRTDKGVSAFQQVISLVVRSNLV
Sbjct:    1 MKNFISSLYIFSKLSFKRCGPISYLSTEENLKLESERMKNRKLRKYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDVNISFCGRTDKGVSAFQQVISLVVRSNLV 129
[Close]
BLAST of tRNA pseudouridine synthase vs. Planmine SMEST
Match: SMESG000063186.1 (SMESG000063186.1)

HSP 1 Score: 307.375 bits (786), Expect = 1.616e-93
Identity = 161/264 (60.98%), Postives = 206/264 (78.03%), Query Frame = 2 
Query:  722 EKEIDYVFILNKFLPEDIKILAWSPVDKDFHATFNCLGRSYKYFFPRGNLSIELMQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHETKMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEXXXXXXXXXXXXXXXKPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQTILKCFLSELNQRHSKKIDSALLLNYINKDVRFENWKPLLERLKESTLEARI-DKHNILMK 1510
            ++EIDYVFILNK LP+DI+ILAWSPVD DF A FN  GRSYKYFFP+ +LSI+LMQEAG +LIGSHDFRNFC+M + QGITQF RNIY V IEP D+ E + E +MY I IS SSFIYHQIRC V +LLMIG  KE  ++ID LLD++GIGGLGKP YQMA +LPLVF+EA Y  ++WQTSE+A N   +++ +LW+ + TKQ++LK FL E+N +HS KI+ + L++ I  D +F+ WKPLL+R KE +L+ ++ + HN  MK
Sbjct:  139 DEEIDYVFILNKVLPKDIRILAWSPVDVDFSARFNSSGRSYKYFFPKSDLSIDLMQEAGKRLIGSHDFRNFCSMQISQGITQFVRNIYEVTIEPFDKSEADSEYRMYVINISASSFIYHQIRCTVAILLMIGQRKESEDVIDHLLDVEGIGGLGKPFYQMASDLPLVFYEASYEGINWQTSEEAVNDYLVDIQNLWVSYQTKQSMLKLFLDEINLQHSLKIEKSSLIDAICNDAKFDKWKPLLKRPKELSLKEKLFNHHNKKMK 402

HSP 2 Score: 108.227 bits (269), Expect = 1.364e-24
Identity = 55/96 (57.29%), Postives = 66/96 (68.75%), Query Frame = 1 
Query:  127 ESERMKNRKLRKYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLFKSKLTEDRDVNISFCGRTDKGVSAFQQVISLVVRSNLV 414
            +S++ K+RKL     +FS YK+RH+ LKFCYLGW Y G+  Q   DN V  K+  +L   KL EDR+ NI  CGRTDKGVSAFQQVISL VRS L 
Sbjct:   28 QSKKNKSRKL-----EFSHYKMRHVVLKFCYLGWSYCGLAQQATVDNTVIEKIINALVLCKLIEDRETNIVICGRTDKGVSAFQQVISLDVRSALA 118
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BLAST of tRNA pseudouridine synthase vs. Planmine SMEST
Match: SMESG000071031.1 (SMESG000071031.1)

HSP 1 Score: 112.079 bits (279), Expect = 9.002e-29
Identity = 66/108 (61.11%), Postives = 73/108 (67.59%), Query Frame = -3 
Query: 3334 VLLDLRNTHLLVHIEKVLWRYQDVDYAGKTYCLTRL--GLYCAPRIMSKILGKVIFSNVAVSDVTSSYIDDIAVDTSKVDLVQVIQLLESFRLATKDPILIVNAKALG 3651
            V+LDLRN +L +HIEK  W Y  V Y+ KTYCLTRL  GL CA R M KI+GKV+ SN+AV   TSSYIDDIAVDTSKVD            LATKDPI  V  KALG
Sbjct:    5 VVLDLRNAYLRIHIEKYFWCYPVVQYSEKTYCLTRLEFGLNCALRKMLKIMGKVLSSNIAVRYATSSYIDDIAVDTSKVD------------LATKDPIPNVILKALG 100
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BLAST of tRNA pseudouridine synthase vs. Planmine SMEST
Match: SMESG000002973.1 (SMESG000002973.1)

HSP 1 Score: 58.151 bits (139), Expect = 4.563e-16
Identity = 28/37 (75.68%), Postives = 30/37 (81.08%), Query Frame = -2 
Query: 2936 SDLDDKNVHSKLHSGRKWRIPKFLLGDTIHDRCKDRK 3046
            + LDD+ V SKLHSG KWRIPKFLLGDTI  RCK RK
Sbjct:  684 AGLDDETVPSKLHSGWKWRIPKFLLGDTILYRCKGRK 720

HSP 2 Score: 46.9802 bits (110), Expect = 4.563e-16
Identity = 23/33 (69.70%), Postives = 24/33 (72.73%), Query Frame = -1 
Query: 2847 GSQAGLYEVGRHVFVK*PSAKCNVPCEA*TVTG 2945
            G +AGLYEVG  VFVK P AK N P EA TVTG
Sbjct:  718 GRKAGLYEVGSQVFVKPPRAKSNAPWEAGTVTG 750
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BLAST of tRNA pseudouridine synthase vs. Planmine SMEST
Match: SMESG000054470.1 (SMESG000054470.1)

HSP 1 Score: 79.337 bits (194), Expect = 2.503e-15
Identity = 49/114 (42.98%), Postives = 65/114 (57.02%), Query Frame = -3 
Query: 3334 NRWEEVVLLDLRNTHLLVHIEKVLWRYQDVDYAGKTYCLTRL--GLYCAPRIMSKILGKVIFSNVAVSDVTSSYIDDIAVDTSKVDLVQVIQLLESFRLATKDPILIVNAKALG 3669
            N  ++VV+LDL+N  + +H+ +   +YQ   Y  K Y LTRL   L CA ++MS +L KV+  N  + D T  YID IAVDTSKV + +V   L    L TK    I N K LG
Sbjct:  101 NGGDQVVMLDLKNAFVQLHVRENHLKYQKSRYKDKFYYLTRLRFELNCASKVMSLVLRKVLNLNPRIKDSTDHYIDGIAVDTSKVQVTEVGNRLAQIGLTTKPTDSIENTKILG 214
The following BLAST results are available for this feature:
BLAST of tRNA pseudouridine synthase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PUS33.801e-4233.46pseudouridine synthase 3 [Source:HGNC Symbol;Acc:H... [more]
PUS33.801e-4233.46pseudouridine synthase 3 [Source:HGNC Symbol;Acc:H... [more]
PUS37.706e-2228.05pseudouridine synthase 3 [Source:HGNC Symbol;Acc:H... [more]
PUS33.819e-1950.60pseudouridine synthase 3 [Source:HGNC Symbol;Acc:H... [more]
PUS32.295e-841.51pseudouridine synthase 3 [Source:HGNC Symbol;Acc:H... [more]
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BLAST of tRNA pseudouridine synthase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
tag-1249.740e-3031.80Probable tRNA pseudouridine synthase tag-124 [Sou... [more]
pus-15.498e-628.28tRNA pseudouridine synthase [Source:UniProtKB/TrE... [more]
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BLAST of tRNA pseudouridine synthase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG30455.115e-3430.63gene:FBgn0034703 transcript:FBtr0071787[more]
CG341402.074e-625.14gene:FBgn0083976 transcript:FBtr0110975[more]
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BLAST of tRNA pseudouridine synthase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pus34.090e-4636.40pseudouridylate synthase 3 [Source:ZFIN;Acc:ZDB-GE... [more]
pus31.939e-2045.63pseudouridylate synthase 3 [Source:ZFIN;Acc:ZDB-GE... [more]
pusl14.723e-925.85pseudouridine synthase like 1 [Source:ZFIN;Acc:ZDB... [more]
pusl12.455e-825.85pseudouridine synthase like 1 [Source:ZFIN;Acc:ZDB... [more]
pusl12.669e-825.85pseudouridine synthase like 1 [Source:ZFIN;Acc:ZDB... [more]
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BLAST of tRNA pseudouridine synthase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
prkd35.795e-4639.25protein kinase D3 [Source:Xenbase;Acc:XB-GENE-6036... [more]
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BLAST of tRNA pseudouridine synthase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Pus31.811e-4333.33pseudouridine synthase 3 [Source:MGI Symbol;Acc:MG... [more]
Pus35.256e-3642.18pseudouridine synthase 3 [Source:MGI Symbol;Acc:MG... [more]
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BLAST of tRNA pseudouridine synthase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9JI38|PUS3_MOUSE1.267e-4233.33tRNA pseudouridine(38/39) synthase OS=Mus musculus... [more]
sp|Q3SX07|PUS3_BOVIN1.895e-4234.07tRNA pseudouridine(38/39) synthase OS=Bos taurus O... [more]
sp|Q9BZE2|PUS3_HUMAN1.825e-4133.46tRNA pseudouridine(38/39) synthase OS=Homo sapiens... [more]
sp|P31115|PUS3_YEAST1.562e-2938.12tRNA pseudouridine(38/39) synthase OS=Saccharomyce... [more]
sp|Q09524|TG124_CAEEL1.239e-2831.80Probable tRNA pseudouridine synthase tag-124 OS=Ca... [more]
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BLAST of tRNA pseudouridine synthase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4Y2QSA25.393e-5040.68tRNA pseudouridine(38/39) synthase OS=Araneus vent... [more]
D2A3755.547e-4938.61tRNA pseudouridine(38/39) synthase-like Protein OS... [more]
A0A0R3UJN54.552e-4841.18tRNA pseudouridine synthase OS=Mesocestoides corti... [more]
A0A087U2S71.533e-4736.73tRNA pseudouridine(38/39) synthase (Fragment) OS=S... [more]
A0A5K3F2C82.313e-4741.18tRNA pseudouridine synthase OS=Mesocestoides corti... [more]
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BLAST of tRNA pseudouridine synthase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
pus31.710e-4935.88pseudouridine synthase 3 [Source:NCBI gene;Acc:103... [more]
pus36.141e-4935.88pseudouridine synthase 3 [Source:NCBI gene;Acc:103... [more]
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BLAST of tRNA pseudouridine synthase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of tRNA pseudouridine synthase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
DEG12.250e-3138.12tRNA:pseudouridine synthase; introduces pseudourid... [more]
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BLAST of tRNA pseudouridine synthase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO360252.705e-4840.64tRNA pseudouridine synthase [Source:UniProtKB/TrE... [more]
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BLAST of tRNA pseudouridine synthase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
pus36.291e-4533.97pseudouridine synthase 3 [Source:NCBI gene;Acc:101... [more]
pus36.291e-4533.97pseudouridine synthase 3 [Source:NCBI gene;Acc:101... [more]
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BLAST of tRNA pseudouridine synthase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000041371.14.183e-17499.26SMESG000041371.1[more]
SMESG000063186.11.616e-9360.98SMESG000063186.1[more]
SMESG000071031.19.002e-2961.11SMESG000071031.1[more]
SMESG000002973.14.563e-1675.68SMESG000002973.1[more]
SMESG000054470.12.503e-1542.98SMESG000054470.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001491 ID=SMED30001491|Name=tRNA pseudouridine synthase|organism=Schmidtea mediterranea sexual|type=transcript|length=3878bp
TTTAAATATTATATAAATATATTAGTTATGAAAAATTTTATCTCATCGCT
GTATATATTTTCGAAACTTAGCTTTAAAAGATGTGGTCCAATTAGTTATT
TGTCAACTGAAGAAAATTTAAAATTAGAAAGTGAGAGAATGAAAAACCGA
AAACTTAGAAAATATTCATTAGATTTTTCAGAGTACAAATTGAGACACAT
TGCTTTAAAGTTCTGTTATTTAGGATGGCGATACAGCGGAGTTATTGGAC
AATACCAGTCCGATAATGTAGTAAAATCAAAATTACAAGAAAGTTTGTTT
AAATCCAAATTAACTGAAGATAGAGATGTTAATATTTCATTCTGTGGAAG
AACTGATAAAGGTGTTAGTGCATTTCAACAAGTAATATCATTAGTTGTCC
GATCGAATTTGGTTTCTGGACGAGGAATTATTGAAAATTCGGGAATTAAT
ATAAATAATAGGCTGAGTATGTTCTATAAGTTTAAATTATAATTTCGCGT
TATTGGCATTTAAAACAGTGTAAAACTTTGACATTGCATTTATATAAAAC
TCCCAATAATAAGCTTAACTTTTTTCACAATTAAAGATTTAATATAAAGA
TAATTCAAGATAAAATTACAGCATTTCATGAATTTAAACTCGAAGGGTGT
CGGGGCATCGAATATTTTCAATCCATATCAATTTCAAGTAAAGCTAGTAA
TTATTTCTATCAGATACCAATGAAAAAGAAATTGATTACGTATTCATTTT
GAATAAGTTCCTACCAGAAGATATCAAGATATTGGCATGGAGTCCTGTCG
ATAAAGATTTTCATGCAACATTCAATTGTTTAGGACGCTCTTATAAATAT
TTCTTCCCCAGAGGAAATTTGTCAATCGAGTTGATGCAAGAAGCCGGTAC
AAAGCTCATAGGATCTCACGACTTTAGAAATTTCTGTACTATGTTAGTTA
AACAAGGAATCACACAATTTACGAGAAATATTTATTCCGTTAAAATCGAA
CCGCTTGACGAAATTGAACCGGAACACGAAACGAAAATGTATGGCATATA
TATTAGTGGCAGCTCGTTTATTTATCATCAAATACGATGCATCGTTGGAG
TTTTATTGATGATCGGAATGGGTAAGGAAAAGGTAGAAATTATTGATCAG
CTTCTGGATATCGATGGAATCGGAGGTTTGGGGAAACCTTCTTACCAGAT
GGCTTTTGAGTTGCCCTTGGTTTTCCACGAGGCCTTGTATAATGACATGG
ATTGGCAGACTAGTGAACAGGCTTCAAATATTATTAATTTAAATCTCCAT
AGCTTATGGCTCAAATTTTATACTAAACAGACTATCTTGAAATGTTTTCT
CAGCGAATTAAACCAAAGGCATTCCAAGAAAATCGATAGTGCACTGCTAT
TGAATTATATTAATAAGGATGTGCGGTTTGAAAATTGGAAACCTCTCCTA
GAACGACTCAAAGAAAGTACTCTTGAAGCAAGAATTGACAAGCATAATAT
TTTGATGAAAGTTGAGATTGATTAATAAATTTAGTCCCCATTTCTGTGTT
CTCCTTTCTATCTAGATATAGGGGAACAAACTTTTGTTTAGGTATCATAT
TTATCAATGAGAGTTGAAAGACTTGAAGTTACATCAATAATCAACACAGC
AAAAGGTGTTAGTGTACTGGTGAGCTTTTGAATGGGCATCTATGTTTCTA
TGAGTACATGGTCCATTTCTTCCCTCCTCTGCCAGTTTACTAGGTTCATT
TCCTCACTTTTATACACCCATTAAGTTCACTTTACCGCTATGTCCCCATG
GGGACCCAACTTGGTACTTTGCGTAACAGCTGAAAACTACCCAAGCATGT
GAGAACTGTTTCCAATACTACTCCTTGAATAACTTTTGTCTTCGGATTCT
TGACTGCCGTGGAAGGACTTATCTCTTAGGGCACCTAAACTTTGATATTT
TGACCGTTTTGCCTATGCCTATACGCGTCCGTGAAATTCGGACGATCCTT
TAGTTTTATCATAATTTACCATTTTACTTGAATTATTGATTTTATGCCCC
ATTCGGAGTTATTTATTTTGAATCTTAATTGGTTTAATTCTAAGCGTTCT
CATTTGATTTTAATGGGTAATTAATAGTACAAGATATTTTATAAATTATT
TATTTTACGAATTGGCTCGACAAAATTAAGACGGGAAAGCAAGTTGCATT
GGAGATCTTCATTTTTCAACAAACGCTCTTTCCTAATTATCATTTTATAT
TTCAGTTTTCAGGAAAACGATCATTCGGCATTTCTCCGGGAAACGTTATT
GGTAACTTTAGCTATCACACAACAATCATAGTTACTTTATTCAAAATTTA
TATTATCACTGAAACATAAGAAACTTGTGGAGCTCTTTTGTACGCTCAAG
CGGCGACATGGTCCTCTTAATACTTTTGTATTTGGTCCGCAAGAGGCGAT
ATTCATCTTCCAATGGAATTGTGGTTGAAGATGTCTCGATATCAGGTGAT
CACTTTCCGCTGATGATGGGAATGCCAGGAAGTTTCATTAGTTGAACCGG
CAAAATGAGTTAAAATATAGTTTTTATTTTAGCCAAACGCTTACAAGATT
TTCGACAACAAAATCAGTAATATTATGATGGCAATAAGAAAAAAATAATG
ACTAAATGGTGACCTCCATTGCATTAAATAAAATATATCTCGCCTTGTAT
CCTATCTATACACATTCTTTACTGACACACTCACGCTACTTTTCATACAA
AGTCGGCATCCATCACTCCGTTATCATTTCTAGTCACTATATTGGCCAGT
AGATTTCCCATAGTGTGTGTATGCTATAGATCGGTGTATTGTGGTTGCCA
GTGACTGTTCAAGCTTCACATGGTACGTTGCATTTTGCGCTCGGTTACTT
CACAAAAACGTGGCGACCAACCTCGTATAGACCAGCTTGCGATCCTTACA
ACGATCGTGAATCGTATCACCTAAGAGAAACTTTGGAATTCTCCATTTTC
GGCCAGAATGTAATTTTGAATGCACATTTTTATCGTCTAAATCTGAATGT
GGAGATTTCTTCCACACGACCTTTCTCTAAGAATTCTACATTTTGGGCGC
TACATACTGATGTTGAATCGTCTAGGTCAAGATTTTTCCACAAGCAGTAT
CTACTTTGTCCACAGTCTACTATAGATAGATAAATATCCTCATTCATGTG
AGTGACGTCGATGGCGATACGAGACCACTCTTCCTCTATTTCCAATGCAC
CCTTTCGACGAGAGCACTGATTATCAACATTGTACTGTATAAGTTGATTT
CCTCTTGACCATCTTAGCTCTTTGCTTTTCGGGCCCCAATGCTTTCGCGT
TCACAATAAGTATTGGGTCTTTGGTTGCCAAACGAAACGACTCGAGTAAT
TGTATTACTTGTACCAGATCAACTTTTGATGTATCTACCGCGATATCATC
GATGTAACTTGAGGTGACGTCACTAACGGCAACGTTAGAAAAGATCACTT
TTCCCAAAATCTTTGACATTATTCTAGGCGCACAGTATAATCCAAGCCGA
GTTAAACAATAGGTTTTTCCGGCATAGTCTACATCCTGATAACGCCATAA
GACTTTTTCAATGTGAACCAGCAAATGAGTGTTACGTAAATCGAGAAGAA
CTACCTCTTCCCATCTGTTGCCACTGCCGGATTGTCTCCGAACAAACATT
ACTGTCCCCTGTGTGGTATTCGACAGATTCATTTAACTTCCGAAAATCCA
TGACTGGTCGGACGTTTCCTTTATTCACTTGTTCCACCGCTAGTAAATGT
ATCACTCCACCGTCGTATTCTCGACCGTATTAGTCAACCGTTTGAAATCC
ACATTTGAATTTCTTTATCAAAGTTCAT
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protein sequence of SMED30001491-orf-1

>SMED30001491-orf-1 ID=SMED30001491-orf-1|Name=SMED30001491-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=214bp
MQEAGTKLIGSHDFRNFCTMLVKQGITQFTRNIYSVKIEPLDEIEPEHET
KMYGIYISGSSFIYHQIRCIVGVLLMIGMGKEKVEIIDQLLDIDGIGGLG
KPSYQMAFELPLVFHEALYNDMDWQTSEQASNIINLNLHSLWLKFYTKQT
ILKCFLSELNQRHSKKIDSALLLNYINKDVRFENWKPLLERLKESTLEAR
IDKHNILMKVEID*
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protein sequence of SMED30001491-orf-2

>SMED30001491-orf-2 ID=SMED30001491-orf-2|Name=SMED30001491-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=164bp
FKYYINILVMKNFISSLYIFSKLSFKRCGPISYLSTEENLKLESERMKNR
KLRKYSLDFSEYKLRHIALKFCYLGWRYSGVIGQYQSDNVVKSKLQESLF
KSKLTEDRDVNISFCGRTDKGVSAFQQVISLVVRSNLVSGRGIIENSGIN
INNRLSMFYKFKL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR001406PsdUridine_synth_TruA
IPR020094PsdUridine_synth_TruA_N
IPR020103PsdUridine_synth_cat_dom_sf
IPR020097PsdUridine_synth_TruA_a/b_dom
IPR020095PsdUridine_synth_TruA_C
Vocabulary: molecular function
TermDefinition
GO:0003723RNA binding
GO:0009982pseudouridine synthase activity
GO:0016853isomerase activity
Vocabulary: biological process
TermDefinition
GO:0009451RNA modification
GO:0001522pseudouridine synthesis
GO:0008033tRNA processing
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020097Pseudouridine synthase I, TruA, alpha/beta domainPFAMPF01416PseudoU_synth_1coord: 7..120
e-value: 1.1E-20
score: 74.1
IPR020095Pseudouridine synthase I, TruA, C-terminalGENE3DG3DSA:3.30.70.660coord: 1..119
e-value: 1.7E-16
score: 62.1
IPR001406Pseudouridine synthase I, TruAPANTHERPTHR11142PSEUDOURIDYLATE SYNTHASEcoord: 1..204
NoneNo IPR availablePANTHERPTHR11142:SF5TRNA PSEUDOURIDINE(38/39) SYNTHASEcoord: 1..204
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILYSSF55120Pseudouridine synthasecoord: 1..127