SMED30001461

Overview


Name	SMED30001461
Smed ID	SMED30001461
Length (bp)	315

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30001461

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
photoreceptor neuron	SMED30001461	SMESG000006530.1	Smed-meis	smed_ncbi_20200123	PMID:22884275 Lapan et al., 2012
pharynx	SMED30001461	SMESG000006530.1	meis	smed_ncbi_20200123	PMID:25254346 Scimone et al., 2014
central nervous system	SMED30001461	SMESG000006530.1	meis	smed_ncbi_20200123	PMID:25254346 Scimone et al., 2014

Note: Hover over icons to view figure legend

Alignments

SMED30001461 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.014489:3203..4992 -	1544

Homology

[Close]

BLAST of SMED30001461 vs. Ensembl Human
Match: MEIS3 (Meis homeobox 3 [Source:HGNC Symbol;Acc:HGNC:29537])

HSP 1 Score: 94.7449 bits (234), Expect = 6.252e-25
Identity = 45/79 (56.96%), Postives = 57/79 (72.15%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKD------STNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            +  K +K EIY HPLFPLLAL+FEKCELAT SP+D       T    DVCSS+SFN+DI AF K++  SE+ LFS++P+
Sbjct:   51 DGLKREKDEIYGHPLFPLLALVFEKCELATCSPRDGAGAGLGTPPGGDVCSSDSFNEDIAAFAKQV-RSERPLFSSNPE 128

[Close]

BLAST of SMED30001461 vs. Ensembl Human
Match: MEIS2 (Meis homeobox 2 [Source:HGNC Symbol;Acc:HGNC:7001])

HSP 1 Score: 92.0485 bits (227), Expect = 2.539e-23
Identity = 41/73 (56.16%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLAL+FEKCELAT +P++   +  DVCSS+SFN+DI  F K++  +EK LFS++P+
Sbjct:   71 DALKRDKDAIYGHPLFPLLALVFEKCELATCTPREPGVAGGDVCSSDSFNEDIAVFAKQV-RAEKPLFSSNPE 142

[Close]

BLAST of SMED30001461 vs. Ensembl Human
Match: MEIS1 (Meis homeobox 1 [Source:HGNC Symbol;Acc:HGNC:7000])

HSP 1 Score: 92.0485 bits (227), Expect = 6.909e-23
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   69 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 140

[Close]

BLAST of SMED30001461 vs. Ensembl Human
Match: MEIS1 (Meis homeobox 1 [Source:HGNC Symbol;Acc:HGNC:7000])

HSP 1 Score: 92.0485 bits (227), Expect = 6.958e-23
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   69 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 140

[Close]

BLAST of SMED30001461 vs. Ensembl Human
Match: MEIS1 (Meis homeobox 1 [Source:HGNC Symbol;Acc:HGNC:7000])

HSP 1 Score: 91.6633 bits (226), Expect = 1.494e-22
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   67 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 138

[Close]

BLAST of SMED30001461 vs. Ensembl Celegans
Match: unc-62 (Homeobox protein unc-62 [Source:UniProtKB/Swiss-Prot;Acc:Q9N5D6])

HSP 1 Score: 72.7886 bits (177), Expect = 2.614e-16
Identity = 35/75 (46.67%), Postives = 54/75 (72.00%), Query Frame = -1

Query:    4 SFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDST---NSANDVCSSESFNDDITAFVKEIM-TSEKDLFSADP 216
            + K DK+ IY HPL+PLL L+FEKCELAT++P+D++   ++++DVCSS SF DD+  FV+     ++K  +  +P
Sbjct:   18 AMKRDKESIYAHPLYPLLVLLFEKCELATSTPRDTSRDGSTSSDVCSSASFKDDLNEFVRHTQENADKQYYVPNP 92

[Close]

BLAST of SMED30001461 vs. Ensembl Celegans
Match: unc-62 (Homeobox protein unc-62 [Source:UniProtKB/Swiss-Prot;Acc:Q9N5D6])

HSP 1 Score: 72.4034 bits (176), Expect = 4.108e-16
Identity = 35/75 (46.67%), Postives = 54/75 (72.00%), Query Frame = -1

Query:    4 SFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDST---NSANDVCSSESFNDDITAFVKEIM-TSEKDLFSADP 216
            + K DK+ IY HPL+PLL L+FEKCELAT++P+D++   ++++DVCSS SF DD+  FV+     ++K  +  +P
Sbjct:   55 AMKRDKESIYAHPLYPLLVLLFEKCELATSTPRDTSRDGSTSSDVCSSASFKDDLNEFVRHTQENADKQYYVPNP 129

[Close]

BLAST of SMED30001461 vs. Ensembl Celegans
Match: unc-62 (Homeobox protein unc-62 [Source:UniProtKB/Swiss-Prot;Acc:Q9N5D6])

HSP 1 Score: 72.4034 bits (176), Expect = 4.350e-16
Identity = 35/75 (46.67%), Postives = 54/75 (72.00%), Query Frame = -1

Query:    4 SFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDST---NSANDVCSSESFNDDITAFVKEIM-TSEKDLFSADP 216
            + K DK+ IY HPL+PLL L+FEKCELAT++P+D++   ++++DVCSS SF DD+  FV+     ++K  +  +P
Sbjct:   92 AMKRDKESIYAHPLYPLLVLLFEKCELATSTPRDTSRDGSTSSDVCSSASFKDDLNEFVRHTQENADKQYYVPNP 166

[Close]

BLAST of SMED30001461 vs. Ensembl Celegans
Match: unc-62 (Homeobox protein unc-62 [Source:UniProtKB/Swiss-Prot;Acc:Q9N5D6])

HSP 1 Score: 72.0182 bits (175), Expect = 4.934e-16
Identity = 35/75 (46.67%), Postives = 54/75 (72.00%), Query Frame = -1

Query:    4 SFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDST---NSANDVCSSESFNDDITAFVKEIM-TSEKDLFSADP 216
            + K DK+ IY HPL+PLL L+FEKCELAT++P+D++   ++++DVCSS SF DD+  FV+     ++K  +  +P
Sbjct:   18 AMKRDKESIYAHPLYPLLVLLFEKCELATSTPRDTSRDGSTSSDVCSSASFKDDLNEFVRHTQENADKQYYVPNP 92

[Close]

BLAST of SMED30001461 vs. Ensembl Celegans
Match: unc-62 (Homeobox protein unc-62 [Source:UniProtKB/Swiss-Prot;Acc:Q9N5D6])

HSP 1 Score: 71.633 bits (174), Expect = 7.506e-16
Identity = 35/75 (46.67%), Postives = 54/75 (72.00%), Query Frame = -1

Query:    4 SFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDST---NSANDVCSSESFNDDITAFVKEIM-TSEKDLFSADP 216
            + K DK+ IY HPL+PLL L+FEKCELAT++P+D++   ++++DVCSS SF DD+  FV+     ++K  +  +P
Sbjct:   92 AMKRDKESIYAHPLYPLLVLLFEKCELATSTPRDTSRDGSTSSDVCSSASFKDDLNEFVRHTQENADKQYYVPNP 166

[Close]

BLAST of SMED30001461 vs. Ensembl Fly
Match: hth (gene:FBgn0001235 transcript:FBtr0344145)

HSP 1 Score: 90.8929 bits (224), Expect = 1.865e-23
Identity = 43/71 (60.56%), Postives = 52/71 (73.24%), Query Frame = -1

Query:    1 FKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 213
             K DK  IY HPLFPLLALIFEKCELAT +P++      DVCSSESFN+DI  F K+I  S+K  ++ADP+
Sbjct:   90 HKRDKDAIYEHPLFPLLALIFEKCELATCTPREPGVQGGDVCSSESFNEDIAMFSKQI-RSQKPYYTADPE 159

[Close]

BLAST of SMED30001461 vs. Ensembl Fly
Match: hth (gene:FBgn0001235 transcript:FBtr0100455)

HSP 1 Score: 90.8929 bits (224), Expect = 1.865e-23
Identity = 43/71 (60.56%), Postives = 52/71 (73.24%), Query Frame = -1

Query:    1 FKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 213
             K DK  IY HPLFPLLALIFEKCELAT +P++      DVCSSESFN+DI  F K+I  S+K  ++ADP+
Sbjct:   90 HKRDKDAIYEHPLFPLLALIFEKCELATCTPREPGVQGGDVCSSESFNEDIAMFSKQI-RSQKPYYTADPE 159

[Close]

BLAST of SMED30001461 vs. Ensembl Fly
Match: hth (gene:FBgn0001235 transcript:FBtr0100454)

HSP 1 Score: 90.5077 bits (223), Expect = 2.728e-23
Identity = 43/71 (60.56%), Postives = 52/71 (73.24%), Query Frame = -1

Query:    1 FKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 213
             K DK  IY HPLFPLLALIFEKCELAT +P++      DVCSSESFN+DI  F K+I  S+K  ++ADP+
Sbjct:   90 HKRDKDAIYEHPLFPLLALIFEKCELATCTPREPGVQGGDVCSSESFNEDIAMFSKQI-RSQKPYYTADPE 159

[Close]

BLAST of SMED30001461 vs. Ensembl Fly
Match: hth (gene:FBgn0001235 transcript:FBtr0082256)

HSP 1 Score: 87.8113 bits (216), Expect = 1.621e-21
Identity = 43/70 (61.43%), Postives = 52/70 (74.29%), Query Frame = -1

Query:    1 KLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 210
            K DK  IY HPLFPLLALIFEKCELAT +P++      DVCSSESFN+DI  F K+I  S+K  ++ADP+
Sbjct:   91 KRDKDAIYEHPLFPLLALIFEKCELATCTPREPGVQGGDVCSSESFNEDIAMFSKQI-RSQKPYYTADPE 159

[Close]

BLAST of SMED30001461 vs. Ensembl Fly
Match: hth (gene:FBgn0001235 transcript:FBtr0082255)

HSP 1 Score: 87.4261 bits (215), Expect = 2.537e-21
Identity = 43/70 (61.43%), Postives = 52/70 (74.29%), Query Frame = -1

Query:    1 KLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 210
            K DK  IY HPLFPLLALIFEKCELAT +P++      DVCSSESFN+DI  F K+I  S+K  ++ADP+
Sbjct:   91 KRDKDAIYEHPLFPLLALIFEKCELATCTPREPGVQGGDVCSSESFNEDIAMFSKQI-RSQKPYYTADPE 159

[Close]

BLAST of SMED30001461 vs. Ensembl Zebrafish
Match: meis2a (Meis homeobox 2a [Source:ZFIN;Acc:ZDB-GENE-020122-2])

HSP 1 Score: 92.8189 bits (229), Expect = 4.547e-25
Identity = 41/71 (57.75%), Postives = 55/71 (77.46%), Query Frame = -1

Query:    1 FKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 213
             K DK +IY HPLFPLLAL+FEKCELAT +P++   +  DVCSS+SFN+DI  F K++  +EK LFS++P+
Sbjct:   68 LKRDKDQIYGHPLFPLLALVFEKCELATCTPREPGVAGGDVCSSDSFNEDIAVFAKQV-RAEKPLFSSNPE 137

[Close]

BLAST of SMED30001461 vs. Ensembl Zebrafish
Match: meis3 (myeloid ecotropic viral integration site 3 [Source:ZFIN;Acc:ZDB-GENE-010406-2])

HSP 1 Score: 93.2041 bits (230), Expect = 1.410e-24
Identity = 48/84 (57.14%), Postives = 61/84 (72.62%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSAN-----------DVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            +S K  + +IY HPLFPLLAL+FEKCELAT SP+DST+ +N           DVCSS+SFNDDI AF K+I  SEK +FS++P+
Sbjct:   40 DSLKHHRDQIYGHPLFPLLALVFEKCELATCSPRDSTSMSNPAHLPGMTNHSDVCSSDSFNDDIAAFAKQI-RSEKPIFSSNPE 122

[Close]

BLAST of SMED30001461 vs. Ensembl Zebrafish
Match: meis1a (Meis homeobox 1 a [Source:ZFIN;Acc:ZDB-GENE-020122-3])

HSP 1 Score: 89.3521 bits (220), Expect = 4.460e-24
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLAL+FEKCELAT +P++S     DVCSSESF++DIT F K+I  +EK  FS++PD
Sbjct:   49 DALKRDKDAIYGHPLFPLLALVFEKCELATCTPRES-GVTGDVCSSESFDEDITVFSKQI-RAEKPFFSSNPD 119

[Close]

BLAST of SMED30001461 vs. Ensembl Zebrafish
Match: meis3 (myeloid ecotropic viral integration site 3 [Source:ZFIN;Acc:ZDB-GENE-010406-2])

HSP 1 Score: 93.5893 bits (231), Expect = 5.616e-24
Identity = 48/84 (57.14%), Postives = 61/84 (72.62%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSAN-----------DVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            +S K  + +IY HPLFPLLAL+FEKCELAT SP+DST+ +N           DVCSS+SFNDDI AF K+I  SEK +FS++P+
Sbjct:   40 DSLKHHRDQIYGHPLFPLLALVFEKCELATCSPRDSTSMSNPAHLPGMTNHSDVCSSDSFNDDIAAFAKQI-RSEKPIFSSNPE 122

[Close]

BLAST of SMED30001461 vs. Ensembl Zebrafish
Match: meis2a (Meis homeobox 2a [Source:ZFIN;Acc:ZDB-GENE-020122-2])

HSP 1 Score: 92.4337 bits (228), Expect = 6.110e-24
Identity = 41/71 (57.75%), Postives = 55/71 (77.46%), Query Frame = -1

Query:    1 FKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 213
             K DK +IY HPLFPLLAL+FEKCELAT +P++   +  DVCSS+SFN+DI  F K++  +EK LFS++P+
Sbjct:   69 LKRDKDQIYGHPLFPLLALVFEKCELATCTPREPGVAGGDVCSSDSFNEDIAVFAKQV-RAEKPLFSSNPE 138

[Close]

BLAST of SMED30001461 vs. Ensembl Xenopus
Match: meis3 (Meis homeobox 3 [Source:NCBI gene;Acc:448474])

HSP 1 Score: 93.9745 bits (232), Expect = 1.202e-23
Identity = 45/75 (60.00%), Postives = 58/75 (77.33%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNS--ANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            +  K +K EIY HPLFPLLAL+FEKCELAT SP+D++ S    DVCSS+SFN+DI  F K++ T EK LFS++P+
Sbjct:   63 DGLKREKDEIYGHPLFPLLALVFEKCELATCSPRDNSGSFPGGDVCSSDSFNEDIAVFAKQVRT-EKPLFSSNPE 136

[Close]

BLAST of SMED30001461 vs. Ensembl Xenopus
Match: MEIS1 (Meis homeobox 1 [Source:NCBI gene;Acc:100488182])

HSP 1 Score: 92.4337 bits (228), Expect = 5.372e-23
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   69 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 140

[Close]

BLAST of SMED30001461 vs. Ensembl Xenopus
Match: MEIS1 (Meis homeobox 1 [Source:NCBI gene;Acc:100488182])

HSP 1 Score: 92.0485 bits (227), Expect = 5.663e-23
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   71 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 142

[Close]

BLAST of SMED30001461 vs. Ensembl Xenopus
Match: MEIS1 (Meis homeobox 1 [Source:NCBI gene;Acc:100488182])

HSP 1 Score: 92.4337 bits (228), Expect = 5.769e-23
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   69 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 140

[Close]

BLAST of SMED30001461 vs. Ensembl Xenopus
Match: MEIS1 (Meis homeobox 1 [Source:NCBI gene;Acc:100488182])

HSP 1 Score: 92.4337 bits (228), Expect = 8.896e-23
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   69 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 140

[Close]

BLAST of SMED30001461 vs. Ensembl Mouse
Match: Meis3 (Meis homeobox 3 [Source:MGI Symbol;Acc:MGI:108519])

HSP 1 Score: 92.4337 bits (228), Expect = 7.069e-25
Identity = 45/79 (56.96%), Postives = 59/79 (74.68%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNS------ANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            +S K +K +IY HPLFPLLAL+FEKCELAT SP+D  ++        DVCSS+SFN+DI AF K+I  SE+ LFS++P+
Sbjct:   54 DSLKREKDDIYGHPLFPLLALVFEKCELATCSPRDGASAGLGSPPGGDVCSSDSFNEDIAAFAKQI-RSERPLFSSNPE 131

[Close]

BLAST of SMED30001461 vs. Ensembl Mouse
Match: Meis1 (Meis homeobox 1 [Source:MGI Symbol;Acc:MGI:104717])

HSP 1 Score: 93.2041 bits (230), Expect = 1.725e-24
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   42 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 113

[Close]

BLAST of SMED30001461 vs. Ensembl Mouse
Match: Meis3 (Meis homeobox 3 [Source:MGI Symbol;Acc:MGI:108519])

HSP 1 Score: 92.4337 bits (228), Expect = 1.829e-23
Identity = 45/79 (56.96%), Postives = 59/79 (74.68%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNS------ANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            +S K +K +IY HPLFPLLAL+FEKCELAT SP+D  ++        DVCSS+SFN+DI AF K+I  SE+ LFS++P+
Sbjct:   54 DSLKREKDDIYGHPLFPLLALVFEKCELATCSPRDGASAGLGSPPGGDVCSSDSFNEDIAAFAKQIR-SERPLFSSNPE 131

[Close]

BLAST of SMED30001461 vs. Ensembl Mouse
Match: Meis1 (Meis homeobox 1 [Source:MGI Symbol;Acc:MGI:104717])

HSP 1 Score: 92.0485 bits (227), Expect = 4.729e-23
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   69 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 140

[Close]

BLAST of SMED30001461 vs. Ensembl Mouse
Match: Meis1 (Meis homeobox 1 [Source:MGI Symbol;Acc:MGI:104717])

HSP 1 Score: 92.0485 bits (227), Expect = 5.030e-23
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   69 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 140

[Close]

BLAST of SMED30001461 vs. UniProt/SwissProt
Match: sp|Q60954|MEIS1_MOUSE (Homeobox protein Meis1 OS=Mus musculus OX=10090 GN=Meis1 PE=1 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 3.308e-22
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   69 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 140

[Close]

BLAST of SMED30001461 vs. UniProt/SwissProt
Match: sp|O00470|MEIS1_HUMAN (Homeobox protein Meis1 OS=Homo sapiens OX=9606 GN=MEIS1 PE=1 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 3.342e-22
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   69 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 140

[Close]

BLAST of SMED30001461 vs. UniProt/SwissProt
Match: sp|Q6DIF3|MEIS3_XENTR (Homeobox protein meis3 OS=Xenopus tropicalis OX=8364 GN=meis3 PE=2 SV=2)

HSP 1 Score: 91.6633 bits (226), Expect = 6.497e-22
Identity = 45/75 (60.00%), Postives = 58/75 (77.33%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNS--ANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            +  K +K EIY HPLFPLLAL+FEKCELAT SP+D++ S    DVCSS+SFN+DI  F K++ T EK LFS++P+
Sbjct:   61 DGLKREKDEIYGHPLFPLLALVFEKCELATCSPRDNSGSFPGGDVCSSDSFNEDIAVFAKQVRT-EKPLFSSNPE 134

[Close]

BLAST of SMED30001461 vs. UniProt/SwissProt
Match: sp|Q5U4X3|MEI3A_XENLA (Homeobox protein meis3-A OS=Xenopus laevis OX=8355 GN=meis3-a PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 6.695e-22
Identity = 45/75 (60.00%), Postives = 58/75 (77.33%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNS--ANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            +  K +K EIY HPLFPLLAL+FEKCELAT SP+D++ S    DVCSS+SFN+DI  F K++ T EK LFS++P+
Sbjct:   61 DGLKREKDEIYGHPLFPLLALVFEKCELATCSPRDNSGSFPGGDVCSSDSFNEDIAVFAKQVRT-EKPLFSSNPE 134

[Close]

BLAST of SMED30001461 vs. UniProt/SwissProt
Match: sp|Q7ZY13|MEI3B_XENLA (Homeobox protein meis3-B OS=Xenopus laevis OX=8355 GN=meis3-b PE=2 SV=2)

HSP 1 Score: 91.6633 bits (226), Expect = 7.483e-22
Identity = 45/75 (60.00%), Postives = 58/75 (77.33%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNS--ANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            +  K +K EIY HPLFPLLAL+FEKCELAT SP+D++ S    DVCSS+SFN+DI  F K++ T EK LFS++P+
Sbjct:   61 DGLKREKDEIYGHPLFPLLALVFEKCELATCSPRDNSGSFPGGDVCSSDSFNEDIAVFAKQVRT-EKPLFSSNPE 134

[Close]

BLAST of SMED30001461 vs. TrEMBL
Match: J7FUV3 (Meis OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 5.884e-47
Identity = 80/80 (100.00%), Postives = 80/80 (100.00%), Query Frame = -1

Query:    1 YIPQEPFNSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 240
            YIPQEPFNSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD
Sbjct:  292 YIPQEPFNSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 371

[Close]

BLAST of SMED30001461 vs. TrEMBL
Match: A0A1S3KHW9 (homeobox protein Meis1-like OS=Salmo salar OX=8030 GN=LOC106560216 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 8.722e-23
Identity = 44/73 (60.27%), Postives = 57/73 (78.08%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK +IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   67 DALKRDKDQIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 138

[Close]

BLAST of SMED30001461 vs. TrEMBL
Match: A0A226N868 (Meis_PKNOX_N domain-containing protein (Fragment) OS=Callipepla squamata OX=9009 GN=ASZ78_003124 PE=4 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 8.848e-23
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   65 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 136

[Close]

BLAST of SMED30001461 vs. TrEMBL
Match: A0A401RJD9 (Meis_PKNOX_N domain-containing protein OS=Chiloscyllium punctatum OX=137246 GN=chiPu_0020755 PE=4 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.610e-22
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            +S K +K  IY HPLFPLLAL+FEKCELAT +P+D   +  DVCSS+SFN+DI  F K+I T EK LFS++P+
Sbjct:   66 DSLKREKDTIYGHPLFPLLALVFEKCELATCTPRDCGMAGGDVCSSDSFNEDIAVFAKQIRT-EKPLFSSNPE 137

[Close]

BLAST of SMED30001461 vs. TrEMBL
Match: A0A4Z2EZ94 (Homeobox protein Meis1 OS=Liparis tanakae OX=230148 GN=meis1 PE=4 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 3.927e-22
Identity = 44/73 (60.27%), Postives = 57/73 (78.08%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++S  +  DVCSS+SFN+DI  F K+I  SE+ LFS++P+
Sbjct:   78 DAVKRDKDAIYGHPLFPLLALIFEKCELATCTPRESGVAGGDVCSSDSFNEDIAVFSKQI-RSERPLFSSNPE 149

[Close]

BLAST of SMED30001461 vs. Ensembl Cavefish
Match: meis1b (Meis homeobox 1 [Source:NCBI gene;Acc:103042376])

HSP 1 Score: 93.2041 bits (230), Expect = 1.774e-23
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   67 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 138

[Close]

BLAST of SMED30001461 vs. Ensembl Cavefish
Match: meis1b (Meis homeobox 1 [Source:NCBI gene;Acc:103042376])

HSP 1 Score: 93.2041 bits (230), Expect = 1.968e-23
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   86 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 157

[Close]

BLAST of SMED30001461 vs. Ensembl Cavefish
Match: meis1b (Meis homeobox 1 [Source:NCBI gene;Acc:103042376])

HSP 1 Score: 93.2041 bits (230), Expect = 2.691e-23
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   86 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 157

[Close]

BLAST of SMED30001461 vs. Ensembl Cavefish
Match: meis2a (homeobox protein Meis1-like [Source:NCBI gene;Acc:103032373])

HSP 1 Score: 90.5077 bits (223), Expect = 1.408e-22
Identity = 43/74 (58.11%), Postives = 55/74 (74.32%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKD-STNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            +S K DK  IY HPLFPLLAL+FEKCELAT +P+D    +  DVCSS SFN+DI  F K+I  +EK LF+++P+
Sbjct:   62 DSLKRDKDHIYGHPLFPLLALVFEKCELATCTPRDPGAGAGGDVCSSHSFNEDIAVFAKQI-RAEKPLFTSNPE 134

[Close]

BLAST of SMED30001461 vs. Ensembl Cavefish
Match: meis2a (Meis homeobox 2 [Source:NCBI gene;Acc:103045180])

HSP 1 Score: 90.5077 bits (223), Expect = 1.551e-22
Identity = 41/71 (57.75%), Postives = 55/71 (77.46%), Query Frame = -1

Query:    1 FKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 213
             K DK +IY HPLFPLLAL+FEKCELAT +P++   +  DVCSS+SFN+DI  F K++  +EK LFS++P+
Sbjct:   73 LKRDKDQIYGHPLFPLLALVFEKCELATCTPREPGVAGGDVCSSDSFNEDIAVFAKQV-RAEKPLFSSNPE 142

[Close]

BLAST of SMED30001461 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001860.1 (pep scaffold:Pmarinus_7.0:GL485367:2529:11155:-1 gene:ENSPMAG00000001690.1 transcript:ENSPMAT00000001860.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 79.7221 bits (195), Expect = 6.558e-21
Identity = 36/61 (59.02%), Postives = 49/61 (80.33%), Query Frame = -1

Query:    1 HPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 183
            HPLFPLLAL+FEKCELAT +P++   +  DVCSS+SFN+DI  F K++  +EK LFS++P+
Sbjct:    1 HPLFPLLALVFEKCELATCTPREPGVAGGDVCSSDSFNEDIAVFAKQV-RAEKPLFSSNPE 60

[Close]

BLAST of SMED30001461 vs. Ensembl Sea Lamprey
Match: pknox2 (pbx/knotted 1 homeobox 2 [Source:ZFIN;Acc:ZDB-GENE-031118-112])

HSP 1 Score: 48.521 bits (114), Expect = 3.017e-8
Identity = 24/78 (30.77%), Postives = 41/78 (52.56%), Query Frame = -1

Query:    1 PQEPFNSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 234
            P +P      DK  IY+HPLFPLL L+  +CE AT   +  T++             ++A ++ ++ ++ +LFS + D
Sbjct:   31 PLDPKAQMDADKNSIYSHPLFPLLRLMMLRCEEATQGAEGITSAG------------LSAEIQAMLGTQSELFSGNGD 96

[Close]

BLAST of SMED30001461 vs. Ensembl Nematostella
Match: EDO36439 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIN3])

HSP 1 Score: 83.9593 bits (206), Expect = 6.615e-21
Identity = 40/68 (58.82%), Postives = 51/68 (75.00%), Query Frame = -1

Query:    7 KLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSAD 210
            K DK+ +Y HPLFPLLALIFEKCELAT +P++      DVCSS SFN+DI AF K+   S+K +FS++
Sbjct:   31 KRDKEVLYGHPLFPLLALIFEKCELATCTPREPGAVGGDVCSSASFNEDIQAFAKQF-KSDKAIFSSN 97

[Close]

BLAST of SMED30001461 vs. Ensembl Nematostella
Match: EDO39375 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAC9])

HSP 1 Score: 51.2174 bits (121), Expect = 4.926e-9
Identity = 27/66 (40.91%), Postives = 39/66 (59.09%), Query Frame = -1

Query:    7 DKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSAD 204
            +K  IY HPLFPLLA++ EKCE AT +        +D  +S++F++DI  F+ +     K  FS D
Sbjct:    4 EKVAIYRHPLFPLLAMLLEKCEHATQT--------SDCPTSDAFDNDIKNFMMQHNREGKPCFSGD 61

[Close]

BLAST of SMED30001461 vs. Ensembl Medaka
Match: meis1b (Meis homeobox 1 [Source:NCBI gene;Acc:101167316])

HSP 1 Score: 92.8189 bits (229), Expect = 1.992e-23
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = -1

Query:    1 NSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 219
            ++ K DK  IY HPLFPLLALIFEKCELAT +P++   +  DVCSSESFN+DI  F K+I  +EK LFS++P+
Sbjct:   68 DALKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQI-RAEKPLFSSNPE 139

[Close]

BLAST of SMED30001461 vs. Ensembl Medaka
Match: meis3 (homeobox protein meis3-B [Source:NCBI gene;Acc:101154966])

HSP 1 Score: 91.6633 bits (226), Expect = 8.324e-23
Identity = 52/92 (56.52%), Postives = 63/92 (68.48%), Query Frame = -1

Query:    1 YIPQEPFNSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSA------------NDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 240
            Y P  P +S K  K  IY HPLFPLLAL+FEKCELAT SP+DS++S             +DVCSSESFNDDI AF K+I  SEK +FS++P+
Sbjct:   63 YGPTLP-DSLKHHKDLIYGHPLFPLLALVFEKCELATCSPRDSSSSLTATSHLPGMTNHSDVCSSESFNDDIAAFAKQI-RSEKPIFSSNPE 152

[Close]

BLAST of SMED30001461 vs. Ensembl Medaka
Match: meis2a (Meis homeobox 2 [Source:NCBI gene;Acc:101174873])

HSP 1 Score: 89.7373 bits (221), Expect = 3.311e-22
Identity = 41/71 (57.75%), Postives = 55/71 (77.46%), Query Frame = -1

Query:    1 FKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 213
             K DK +IY HPLFPLLAL+FEKCELAT +P++   +  DVCSS+SFN+DI  F K++  +EK LFS++P+
Sbjct:   71 LKRDKDQIYGHPLFPLLALVFEKCELATCTPREPGVAGGDVCSSDSFNEDIAVFAKQV-RAEKPLFSSNPE 140

[Close]

BLAST of SMED30001461 vs. Ensembl Medaka
Match: meis2a (Meis homeobox 2 [Source:NCBI gene;Acc:101174873])

HSP 1 Score: 89.3521 bits (220), Expect = 4.026e-22
Identity = 41/71 (57.75%), Postives = 55/71 (77.46%), Query Frame = -1

Query:    1 FKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 213
             K DK +IY HPLFPLLAL+FEKCELAT +P++   +  DVCSS+SFN+DI  F K++  +EK LFS++P+
Sbjct:   70 LKRDKDQIYGHPLFPLLALVFEKCELATCTPREPGVAGGDVCSSDSFNEDIAVFAKQV-RAEKPLFSSNPE 139

[Close]

BLAST of SMED30001461 vs. Ensembl Medaka
Match: meis2a (Meis homeobox 2 [Source:NCBI gene;Acc:101174873])

HSP 1 Score: 89.3521 bits (220), Expect = 5.462e-22
Identity = 41/71 (57.75%), Postives = 55/71 (77.46%), Query Frame = -1

Query:    1 FKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 213
             K DK +IY HPLFPLLAL+FEKCELAT +P++   +  DVCSS+SFN+DI  F K++  +EK LFS++P+
Sbjct:   71 LKRDKDQIYGHPLFPLLALVFEKCELATCTPREPGVAGGDVCSSDSFNEDIAVFAKQV-RAEKPLFSSNPE 140

[Close]

BLAST of SMED30001461 vs. Planmine SMEST
Match: SMESG000006530.1 (SMESG000006530.1)

HSP 1 Score: 182.57 bits (462), Expect = 2.675e-55
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = -1

Query:    1 PYLESISNSGIXXXXXXXXXXXXSYIPQEPFNSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 312
            PYLESISNSGINQYSNNSLSNNSSYIPQEPFNSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD
Sbjct:   90 PYLESISNSGINQYSNNSLSNNSSYIPQEPFNSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 193

[Close]

BLAST of SMED30001461 vs. Planmine SMEST
Match: SMESG000006530.1 (SMESG000006530.1)

HSP 1 Score: 182.57 bits (462), Expect = 2.724e-55
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = -1

Query:    1 PYLESISNSGIXXXXXXXXXXXXSYIPQEPFNSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 312
            PYLESISNSGINQYSNNSLSNNSSYIPQEPFNSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD
Sbjct:   90 PYLESISNSGINQYSNNSLSNNSSYIPQEPFNSFKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 193

[Close]

BLAST of SMED30001461 vs. Planmine SMEST
Match: SMESG000078684.1 (SMESG000078684.1)

HSP 1 Score: 72.4034 bits (176), Expect = 4.263e-16
Identity = 39/69 (56.52%), Postives = 55/69 (79.71%), Query Frame = -1

Query:    7 FKLDKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSAD 213
            FK +K+ IY+H LFPLLA+IFEKCELAT +PK S++S +   SSESFN+D+  F KE++TS+K ++S +
Sbjct:   93 FKGEKESIYSHSLFPLLAVIFEKCELATYTPKYSSSSMDIC-SSESFNEDVMVFAKELVTSDKKIYSQN 160

[Close]

BLAST of SMED30001461 vs. Planmine SMEST
Match: SMESG000013307.1 (SMESG000013307.1)

HSP 1 Score: 49.6766 bits (117), Expect = 4.134e-8
Identity = 25/68 (36.76%), Postives = 40/68 (58.82%), Query Frame = -1

Query:    1 DKKEIYNHPLFPLLALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLFSADPD 204
            +KK IY HPL+P+L+L+ E+CE AT SP        D  S ++F  D+ +++     +EK  F+ + D
Sbjct:   25 EKKSIYGHPLYPILSLLLEQCEQATASP--------DSQSPDTFEADLQSYILRNDNNEKYFFTDNKD 84

The following BLAST results are available for this feature:

BLAST of SMED30001461 vs. Ensembl Human
BLAST of SMED30001461 vs. Ensembl Celegans
BLAST of SMED30001461 vs. Ensembl Fly
BLAST of SMED30001461 vs. Ensembl Zebrafish
BLAST of SMED30001461 vs. Ensembl Xenopus
BLAST of SMED30001461 vs. Ensembl Mouse
BLAST of SMED30001461 vs. UniProt/SwissProt
BLAST of SMED30001461 vs. TrEMBL
BLAST of SMED30001461 vs. Ensembl Cavefish
BLAST of SMED30001461 vs. Ensembl Sea Lamprey
BLAST of SMED30001461 vs. Ensembl Yeast
BLAST of SMED30001461 vs. Ensembl Nematostella
BLAST of SMED30001461 vs. Ensembl Medaka
BLAST of SMED30001461 vs. Planmine SMEST

BLAST of SMED30001461 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
MEIS3	6.252e-25	56.96	Meis homeobox 3 [Source:HGNC Symbol;Acc:HGNC:29537...	[more]
MEIS2	2.539e-23	56.16	Meis homeobox 2 [Source:HGNC Symbol;Acc:HGNC:7001]	[more]
MEIS1	6.909e-23	60.27	Meis homeobox 1 [Source:HGNC Symbol;Acc:HGNC:7000]	[more]
MEIS1	6.958e-23	60.27	Meis homeobox 1 [Source:HGNC Symbol;Acc:HGNC:7000]	[more]
MEIS1	1.494e-22	60.27	Meis homeobox 1 [Source:HGNC Symbol;Acc:HGNC:7000]	[more]

BLAST of SMED30001461 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
unc-62	2.614e-16	46.67	Homeobox protein unc-62 [Source:UniProtKB/Swiss-P...	[more]
unc-62	4.108e-16	46.67	Homeobox protein unc-62 [Source:UniProtKB/Swiss-P...	[more]
unc-62	4.350e-16	46.67	Homeobox protein unc-62 [Source:UniProtKB/Swiss-P...	[more]
unc-62	4.934e-16	46.67	Homeobox protein unc-62 [Source:UniProtKB/Swiss-P...	[more]
unc-62	7.506e-16	46.67	Homeobox protein unc-62 [Source:UniProtKB/Swiss-P...	[more]

BLAST of SMED30001461 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
hth	1.865e-23	60.56	gene:FBgn0001235 transcript:FBtr0344145	[more]
hth	1.865e-23	60.56	gene:FBgn0001235 transcript:FBtr0100455	[more]
hth	2.728e-23	60.56	gene:FBgn0001235 transcript:FBtr0100454	[more]
hth	1.621e-21	61.43	gene:FBgn0001235 transcript:FBtr0082256	[more]
hth	2.537e-21	61.43	gene:FBgn0001235 transcript:FBtr0082255	[more]

BLAST of SMED30001461 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
meis2a	4.547e-25	57.75	Meis homeobox 2a [Source:ZFIN;Acc:ZDB-GENE-020122-...	[more]
meis3	1.410e-24	57.14	myeloid ecotropic viral integration site 3 [Source...	[more]
meis1a	4.460e-24	60.27	Meis homeobox 1 a [Source:ZFIN;Acc:ZDB-GENE-020122...	[more]
meis3	5.616e-24	57.14	myeloid ecotropic viral integration site 3 [Source...	[more]
meis2a	6.110e-24	57.75	Meis homeobox 2a [Source:ZFIN;Acc:ZDB-GENE-020122-...	[more]

BLAST of SMED30001461 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
meis3	1.202e-23	60.00	Meis homeobox 3 [Source:NCBI gene;Acc:448474]	[more]
MEIS1	5.372e-23	60.27	Meis homeobox 1 [Source:NCBI gene;Acc:100488182]	[more]
MEIS1	5.663e-23	60.27	Meis homeobox 1 [Source:NCBI gene;Acc:100488182]	[more]
MEIS1	5.769e-23	60.27	Meis homeobox 1 [Source:NCBI gene;Acc:100488182]	[more]
MEIS1	8.896e-23	60.27	Meis homeobox 1 [Source:NCBI gene;Acc:100488182]	[more]

BLAST of SMED30001461 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Meis3	7.069e-25	56.96	Meis homeobox 3 [Source:MGI Symbol;Acc:MGI:108519]	[more]
Meis1	1.725e-24	60.27	Meis homeobox 1 [Source:MGI Symbol;Acc:MGI:104717]	[more]
Meis3	1.829e-23	56.96	Meis homeobox 3 [Source:MGI Symbol;Acc:MGI:108519]	[more]
Meis1	4.729e-23	60.27	Meis homeobox 1 [Source:MGI Symbol;Acc:MGI:104717]	[more]
Meis1	5.030e-23	60.27	Meis homeobox 1 [Source:MGI Symbol;Acc:MGI:104717]	[more]

BLAST of SMED30001461 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q60954\|MEIS1_MOUSE	3.308e-22	60.27	Homeobox protein Meis1 OS=Mus musculus OX=10090 GN...	[more]
sp\|O00470\|MEIS1_HUMAN	3.342e-22	60.27	Homeobox protein Meis1 OS=Homo sapiens OX=9606 GN=...	[more]
sp\|Q6DIF3\|MEIS3_XENTR	6.497e-22	60.00	Homeobox protein meis3 OS=Xenopus tropicalis OX=83...	[more]
sp\|Q5U4X3\|MEI3A_XENLA	6.695e-22	60.00	Homeobox protein meis3-A OS=Xenopus laevis OX=8355...	[more]
sp\|Q7ZY13\|MEI3B_XENLA	7.483e-22	60.00	Homeobox protein meis3-B OS=Xenopus laevis OX=8355...	[more]

BLAST of SMED30001461 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
J7FUV3	5.884e-47	100.00	Meis OS=Schmidtea mediterranea OX=79327 PE=2 SV=1	[more]
A0A1S3KHW9	8.722e-23	60.27	homeobox protein Meis1-like OS=Salmo salar OX=8030...	[more]
A0A226N868	8.848e-23	60.27	Meis_PKNOX_N domain-containing protein (Fragment) ...	[more]
A0A401RJD9	2.610e-22	60.27	Meis_PKNOX_N domain-containing protein OS=Chiloscy...	[more]
A0A4Z2EZ94	3.927e-22	60.27	Homeobox protein Meis1 OS=Liparis tanakae OX=23014...	[more]

BLAST of SMED30001461 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
meis1b	1.774e-23	60.27	Meis homeobox 1 [Source:NCBI gene;Acc:103042376]	[more]
meis1b	1.968e-23	60.27	Meis homeobox 1 [Source:NCBI gene;Acc:103042376]	[more]
meis1b	2.691e-23	60.27	Meis homeobox 1 [Source:NCBI gene;Acc:103042376]	[more]
meis2a	1.408e-22	58.11	homeobox protein Meis1-like [Source:NCBI gene;Acc:...	[more]
meis2a	1.551e-22	57.75	Meis homeobox 2 [Source:NCBI gene;Acc:103045180]	[more]

BLAST of SMED30001461 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2


Match Name	E-value	Identity	Description
ENSPMAT00000001860.1	6.558e-21	59.02	pep scaffold:Pmarinus_7.0:GL485367:2529:11155:-1 g...	[more]
pknox2	3.017e-8	30.77	pbx/knotted 1 homeobox 2 [Source:ZFIN;Acc:ZDB-GENE...	[more]

BLAST of SMED30001461 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30001461 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO36439	6.615e-21	58.82	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO39375	4.926e-9	40.91	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30001461 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
meis1b	1.992e-23	60.27	Meis homeobox 1 [Source:NCBI gene;Acc:101167316]	[more]
meis3	8.324e-23	56.52	homeobox protein meis3-B [Source:NCBI gene;Acc:101...	[more]
meis2a	3.311e-22	57.75	Meis homeobox 2 [Source:NCBI gene;Acc:101174873]	[more]
meis2a	4.026e-22	57.75	Meis homeobox 2 [Source:NCBI gene;Acc:101174873]	[more]
meis2a	5.462e-22	57.75	Meis homeobox 2 [Source:NCBI gene;Acc:101174873]	[more]

BLAST of SMED30001461 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4


Match Name	E-value	Identity	Description
SMESG000006530.1	2.675e-55	100.00	SMESG000006530.1	[more]
SMESG000006530.1	2.724e-55	100.00	SMESG000006530.1	[more]
SMESG000078684.1	4.263e-16	56.52	SMESG000078684.1	[more]
SMESG000013307.1	4.134e-8	36.76	SMESG000013307.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30001461 ID=SMED30001461|Name=SMED30001461|organism=Schmidtea mediterranea sexual|type=transcript|length=315bp
GTCAGGATCTGCGCTGAAAAGATCTTTTTCTGATGTCATAATTTCTTTTA
CAAAAGCTGTGATGTCATCATTGAATGATTCAGATGAGCAAACATCATTT
GCTGAATTGGTTGAATCTTTTGGTGAAGTTGTTGCTAATTCACATTTTTC
GAAAATCAACGCCAAAAGAGGAAAAAGTGGATGATTGTAAATTTCTTTTT
TGTCCAATTTGAAACTATTAAACGGCTCTTGAGGAATATATGATGAATTA
TTTGAGAGAGAATTATTAGAATATTGATTGATTCCAGAATTCGAAATCGA
TTCCAAATATGGATT

protein sequence of SMED30001461-orf-1

>SMED30001461-orf-1 ID=SMED30001461-orf-1|Name=SMED30001461-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=105bp
NPYLESISNSGINQYSNNSLSNNSSYIPQEPFNSFKLDKKEIYNHPLFPL
LALIFEKCELATTSPKDSTNSANDVCSSESFNDDITAFVKEIMTSEKDLF
SADPD

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000017	photoreceptor neuron
PLANA:0000103	central nervous system

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR11850:SF266	HOMEOBOX PROTEIN HOMOTHORAX	coord: 13..104
None	No IPR available	PANTHER	PTHR11850	HOMEOBOX PROTEIN TRANSCRIPTION FACTORS	coord: 13..104

Main menu

SMED30001461

Zeng et. al., 2018

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Owlery

blazegraph-runner