SMED30001457
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30001457 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of SMED30001457 vs. TrEMBL
Match: A0A1I8I0S4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig024983g1 PE=4 SV=1) HSP 1 Score: 99.7525 bits (247), Expect = 3.773e-20 Identity = 77/262 (29.39%), Postives = 130/262 (49.62%), Query Frame = 1 Query: 226 WEKTIF-----NISLFVSNKTKFIVLEYLLDDSNQINGIKV-----AHVNNSDQIWCLMNMRGGLWEICDELSERSRSNS----FCNQYDKIKDNKRCFNYL--YRS-RDFSSETYAYENKN-LYFLKMQNNVIACAIMVLICXXXXXXXXXXXXIWQLVLCGMVASVLFAMSALFSVFASAIQNEKV----KLLQVDE--QCHNYTPLNIPSEICPYYTSKSFSATTEWPFTLSLISSILFAGSTLLWVMITRNLRLVKAK 939 WE F N +L + T++ L +S Q G+ + + N + +CL+N+ +W +CD ++ S +NS +C+ Y + N CF+Y+ Y S RD +T EN + +KMQNN +AC I+VL+C + L+ ++V MV SVL + +F VF+++IQ+EKV K Q+D QC Y+ + P C S SA+ W + +++ + + W+ +TR +R+ K+K Sbjct: 58 WESVTFDFGQVNRTLAAAGSTQWQQPRPLFSESGQQTGLVLLPAGNSTANGTATGFCLLNVYASMWSLCDRVNSASGTNSDYSDYCSTYSMLIRNNTCFSYISGYSSIRDLLDKT---ENSSESQIMKMQNNAMACCIVVLLCVAASMVTGAIGLLARVVPSIMVTSVLNLTAGIFIVFSNSIQHEKVARMIKKPQIDACPQCACYSLIKTPEMFC---NSTHRSASLGWSVITAWVAAGAYLATAGCWLALTRRIRIEKSK 313
BLAST of SMED30001457 vs. TrEMBL
Match: A0A1I8HCJ1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 99.7525 bits (247), Expect = 3.811e-20 Identity = 77/262 (29.39%), Postives = 130/262 (49.62%), Query Frame = 1 Query: 226 WEKTIF-----NISLFVSNKTKFIVLEYLLDDSNQINGIKV-----AHVNNSDQIWCLMNMRGGLWEICDELSERSRSNS----FCNQYDKIKDNKRCFNYL--YRS-RDFSSETYAYENKN-LYFLKMQNNVIACAIMVLICXXXXXXXXXXXXIWQLVLCGMVASVLFAMSALFSVFASAIQNEKV----KLLQVDE--QCHNYTPLNIPSEICPYYTSKSFSATTEWPFTLSLISSILFAGSTLLWVMITRNLRLVKAK 939 WE F N +L + T++ L +S Q G+ + + N + +CL+N+ +W +CD ++ S +NS +C+ Y + N CF+Y+ Y S RD +T EN + +KMQNN +AC I+VL+C + L+ ++V MV SVL + +F VF+++IQ+EKV K Q+D QC Y+ + P C S SA+ W + +++ + + W+ +TR +R+ K+K Sbjct: 58 WESVTFDFGQVNRTLAAAGSTQWQQPRPLFSESGQQTGLVLLPAGNSTANGTATGFCLLNVYASMWSLCDRVNSASATNSDYSDYCSTYSMLIRNNTCFSYISGYSSIRDLLDKT---ENSSESQIMKMQNNAMACCIVVLLCVAASMVTGAIGLLARVVPSIMVTSVLNLTAGIFIVFSNSIQHEKVARMIKKPQIDACPQCACYSLIKTPEMFC---NSSHRSASLGWSVITAWVAAGAYLATAGCWLALTRRIRIEKSK 313
BLAST of SMED30001457 vs. TrEMBL
Match: A0A267GF00 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig024983g2 PE=4 SV=1) HSP 1 Score: 98.9821 bits (245), Expect = 7.751e-20 Identity = 77/262 (29.39%), Postives = 130/262 (49.62%), Query Frame = 1 Query: 226 WEKTIF-----NISLFVSNKTKFIVLEYLLDDSNQINGIKV-----AHVNNSDQIWCLMNMRGGLWEICDELSERSRSNS----FCNQYDKIKDNKRCFNYL--YRS-RDFSSETYAYENKN-LYFLKMQNNVIACAIMVLICXXXXXXXXXXXXIWQLVLCGMVASVLFAMSALFSVFASAIQNEKV----KLLQVDE--QCHNYTPLNIPSEICPYYTSKSFSATTEWPFTLSLISSILFAGSTLLWVMITRNLRLVKAK 939 WE F N +L + T++ L +S Q G+ + + N + +CL+N+ +W +CD ++ S +NS +C+ Y + N CF+Y+ Y S RD +T EN + +KMQNN +AC I+VL+C + L+ ++V MV SVL + +F VF+++IQ+EKV K Q+D QC Y+ + P C S SA+ W + +++ + + W+ +TR +R+ K+K Sbjct: 58 WESVTFDFGQVNRTLAAAGSTQWQQPRPLFSESGQQTGLVLLPAGNSTANGTATGFCLLNVYASMWSLCDRVNSASATNSDYSDYCSTYSMLIRNNTCFSYISGYSSIRDLLDKT---ENSSESQIMKMQNNAMACCIVVLLCVAASMVTGAIGLLARVVPSIMVTSVLNLTAGIFIVFSNSIQHEKVARMIKKPQIDACPQCVCYSLIKTPEMFC---NSTHRSASLGWSVITAWVAAGAYLATAGCWLALTRRIRIEKSK 313
BLAST of SMED30001457 vs. TrEMBL
Match: A0A4S2LVP6 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_005965 PE=4 SV=1) HSP 1 Score: 79.7221 bits (195), Expect = 1.080e-12 Identity = 55/199 (27.64%), Postives = 97/199 (48.74%), Query Frame = 1 Query: 352 DQIWCLMNMRGGLWEICDELSERSRSNSFCNQYDKIKDNKRCFNYLY-RSRDFSSETYAYENKNL---------YFLKMQNNVIACAIMVLICXXXXXXXXXXXXIWQLVLCGMVASVLFAMSALFSVFASAIQNEKVKLLQVD-EQCHNYTPLNIPSEICPYYTSKSFSATTEWPFTLSLISSILFAGSTLLWVMITR 915 + W +MN G+W + L + SF Y Y Y ++ SS +Y +NL Y+LKMQNN+I+C I+V +C + + + +++++ V MV VL S +F +FA+ I + K L+ D CH + ++P E+ Y + S + WP +S I+S +F +++ W++ T+ Sbjct: 223 ESAWIVMNYHAGIWSMQHTLPD-IYDTSFLPHY-----------YTYCKTPPISSSKLSYFAQNLSESGISCTQYYLKMQNNIISCVIVVYLCMVGSALIGTFAIVFRTVPASMVTGVLHVTSGVFVIFANCIHHTKFNRLEYDWGPCHPIS--HLPKEL---YHPEYISVHSHWPLIVSWIASPIFFIASVAWIIFTQ 404
BLAST of SMED30001457 vs. TrEMBL
Match: A0A4Z2DXF0 (Uncharacterized protein (Fragment) OS=Schistosoma japonicum OX=6182 GN=EWB00_000737 PE=4 SV=1) HSP 1 Score: 76.6406 bits (187), Expect = 1.430e-12 Identity = 54/200 (27.00%), Postives = 97/200 (48.50%), Query Frame = 1 Query: 358 IWCLMNMRGGLWEICDELSERSRSNSFCNQYDKIKDNK---RCFNYLYRSRDFSSETYAYENKNL---YFLKMQNNVIACAIMVLICXXXXXXXXXXXXIWQLVLCGMVASVLFAMSALFSVFASAIQNEKVKLLQVDEQCHNYTPLNIPSEICPYYTSKSFSATTEWPFTLSLISSILFAGSTLLWVMITRNLRLVKAK 939 +W +MN++ G+W +C L++ + + D+ C +YL + E KNL + LKMQNN+I+C ++V + + + S +++ V MV VL+ S +F +FA+ I + K+ L+ E Y IP + YT S WP +S ISS +F +++ W+++T+ + +K Sbjct: 30 LWLIMNIQAGIWTMCFRLADAYKPKILAYSSNLHPDSNIPLDCMSYLQANLS--------ETKNLCVQFLLKMQNNIISCVVVVYLSVAASVLIGAFSTLFRAVPAAMVTGVLYVTSGVFIIFANCIHHTKLNRLEY-EWGPCYPISQIPRTL---YTPDIISVHALWPVLVSWISSFVFFTASITWIILTQLMTFENSK 217
BLAST of SMED30001457 vs. Planmine SMEST
Match: SMESG000022121.1 (SMESG000022121.1) HSP 1 Score: 551.206 bits (1419), Expect = 0.000e+0 Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 1 Query: 100 MSFNLEICFPTHSRNTLFLFIICWLLPILATVFYSVVIILPNWEKTIFNISLFVSNKTKFIVLEYLLDDSNQINGIKVAHVNNSDQIWCLMNMRGGLWEICDELSERSRSNSFCNQYDKIKDNKRCFNYLYRSRDFSSETYAYENKNLYFLKMQNNVIACAIMVLICXXXXXXXXXXXXIWQLVLCGMVASVLFAMSALFSVFASAIQNEKVKLLQVDEQCHNYTPLNIPSEICPYYTSKSFSATTEWPFTLSLISSILFAGSTLLWVMITRNLRLVKAKAYL 948 MSFNLEICFPTHSRNTLFLFIICWLLPILATVFYSVVIILPNWEKTIFNISLFVSNKTKFIVLEYLLDDSNQINGIKVAHVNNSDQIWCLMNMRGGLWEICDELSERSRSNSFCNQYDKIKDNKRCFNYLYRSRDFSSETYAYENKNLYFLKMQNNVIACAIMVLICSLFAFGLALASLIWQLVLCGMVASVLFAMSALFSVFASAIQNEKVKLLQVDEQCHNYTPLNIPSEICPYYTSKSFSATTEWPFTLSLISSILFAGSTLLWVMITRNLRLVKAKAYL Sbjct: 1 MSFNLEICFPTHSRNTLFLFIICWLLPILATVFYSVVIILPNWEKTIFNISLFVSNKTKFIVLEYLLDDSNQINGIKVAHVNNSDQIWCLMNMRGGLWEICDELSERSRSNSFCNQYDKIKDNKRCFNYLYRSRDFSSETYAYENKNLYFLKMQNNVIACAIMVLICSLFAFGLALASLIWQLVLCGMVASVLFAMSALFSVFASAIQNEKVKLLQVDEQCHNYTPLNIPSEICPYYTSKSFSATTEWPFTLSLISSILFAGSTLLWVMITRNLRLVKAKAYL 283
BLAST of SMED30001457 vs. Planmine SMEST
Match: SMESG000022121.1 (SMESG000022121.1) HSP 1 Score: 239.58 bits (610), Expect = 5.981e-82 Identity = 131/132 (99.24%), Postives = 132/132 (100.00%), Query Frame = 1 Query: 553 KMQNNVIACAIMVLICXXXXXXXXXXXXIWQLVLCGMVASVLFAMSALFSVFASAIQNEKVKLLQVDEQCHNYTPLNIPSEICPYYTSKSFSATTEWPFTLSLISSILFAGSTLLWVMITRNLRLVKAKAYL 948 +MQNNVIACAIMVLICSLFAFGLALASLIWQLVLCGMVASVLFAMSALFSVFASAIQNEKVKLLQVDEQCHNYTPLNIPSEICPYYTSKSFSATTEWPFTLSLISSILFAGSTLLWVMITRNLRLVKAKAYL Sbjct: 39 QMQNNVIACAIMVLICSLFAFGLALASLIWQLVLCGMVASVLFAMSALFSVFASAIQNEKVKLLQVDEQCHNYTPLNIPSEICPYYTSKSFSATTEWPFTLSLISSILFAGSTLLWVMITRNLRLVKAKAYL 170 HSP 2 Score: 84.3445 bits (207), Expect = 5.981e-82 Identity = 39/40 (97.50%), Postives = 40/40 (100.00%), Query Frame = 2 Query: 404 MNCQKGQDQIRFAINMTKSRTTRDALIICTDQEISVQKRM 523 MNCQKGQDQIRFAINMTKSRTTRDALIICTDQEISVQK+M Sbjct: 1 MNCQKGQDQIRFAINMTKSRTTRDALIICTDQEISVQKQM 40 The following BLAST results are available for this feature:
BLAST of SMED30001457 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30001457 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30001457 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30001457 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30001457 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30001457 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30001457 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30001457 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30001457 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30001457 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30001457 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30001457 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30001457 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30001457 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30001457 ID=SMED30001457|Name=SMED30001457|organism=Schmidtea mediterranea sexual|type=transcript|length=1038bpback to top protein sequence of SMED30001457-orf-1 >SMED30001457-orf-1 ID=SMED30001457-orf-1|Name=SMED30001457-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=284bp MSFNLEICFPTHSRNTLFLFIICWLLPILATVFYSVVIILPNWEKTIFNIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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