SMEDWI-3
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Expression Details SMEDWI-3 ( piwi-3 ) is expressed during Stage 2, Stage 3, Stage 4, Stage 5, Stage 6, Stage 7 and Stage 8 in the neoblast and blastomere
Click to see image symbols and abbreviations
piwi-1+ blastomeres co-express the adult asexual neoblast enriched gene piwi-3. SPIM reconstructed S4 embryo costained with piwi-1 (red) and piwi-3 (green). piwi-1+ blastomeres co-express the nuage factor piwi-3, and virtually all piwi-3+ cells co-express piwi-1.
piwi-1+ blastomeres co-express the adult asexual neoblast enriched gene piwi-3. SPIM reconstructed S3 embryo costained with piwi-1 (green) and piwi-3 (red). piwi-1+ blastomeres co-express the nuage factor piwi-3, and virtually all piwi-3+ cells co-express piwi-1. Several fluorescent beads used for 3-dimensional reconstruction are visible (red).
piwi-1+ blastomeres co-express the adult asexual neoblast enriched gene piwi-3. SPIM reconstructed S5 embryo costained with piwi-1 (green) and piwi-3 (red). piwi-1+ blastomeres co-express the nuage factor piwi-3, and virtually all piwi-3+ cells co-express piwi-1. Several fluorescent beads used for 3-dimensional reconstruction are visible (red). Anatomical Expression
PAGE et. al., 2020SMED30001362 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 57Note: Hover over icons to view figure legend
Homology
BLAST of SMEDWI-3 vs. Ensembl Human
Match: PIWIL1 (piwi like RNA-mediated gene silencing 1 [Source:HGNC Symbol;Acc:HGNC:9007]) HSP 1 Score: 568.54 bits (1464), Expect = 0.000e+0 Identity = 295/766 (38.51%), Postives = 465/766 (60.70%), Query Frame = 1
Query: 736 LSANYVKVRTTQPH--IYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXS-ECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEI-NKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKG-GEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
LS N+ ++ T++P +YQYH+ + P +++R +R ++ DL + + AFDG L++P+ L+ K T + + V + S C YN++F+R+ + + +IGR+Y+ P I + R+ IWPG+ TSI ++ ++L DVSHKV+R+++V D M ++T +K Q Q + +L+G +VLT +NN+ YR+ DIDW +N S F K G ++S+ +Y++ Y +I DL+QP++VS+ R RGP G+ G LIPELC LTGLTD M DF +M++L H R+TP++R + +D I+ + ++ L WG++ + + ++ + + E I+ G + + A++ + +I ++NW+LI + AN L+Q L +P +G+ K ++E++ DRTE YL+ L++ V Q+VV +LS+ R+D+Y+AIKK+ DCP PSQ V+A+TL + + ++A IALQ+N K+GGELW V IP+K M+VG+D +HD RS+ V S N TRWFS+ Q++ +E++ L+ C+ + L+A+ N ++P I +YRDGV DGQL + E+ QF +S Y P + VV+KR+NTR + ++ G++ NP PGT++D VTR YDFF+VSQ+VR G+V+PTHY V+ DNS P +Q + YK CHIYYNWPG +RVPAPC YAHKLA++VGQ++ EP+ L +RL+YL
Sbjct: 114 LSTNHFRL-TSRPQWALYQYHIDYNPLMEARRLRSALL--FQHEDL--IGKCHAFDGTILFLPKRLQQKVTEVFSKTRNGEDVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPNDPIDIPSHRLVIWPGFTTSILQYENSIMLCTDVSHKVLRSETVLDFMFNFYHQTEEHKFQEQVS--KELIGLVVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRR----RGPGGTLP---GPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRKAIMIEVD-DRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVGIDCYHDMTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFA---QSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDNSGLKPDHIQRLTYKLCHIYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL 861
BLAST of SMEDWI-3 vs. Ensembl Human
Match: PIWIL1 (piwi like RNA-mediated gene silencing 1 [Source:HGNC Symbol;Acc:HGNC:9007]) HSP 1 Score: 568.54 bits (1464), Expect = 0.000e+0 Identity = 295/766 (38.51%), Postives = 465/766 (60.70%), Query Frame = 1
Query: 736 LSANYVKVRTTQPH--IYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXS-ECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEI-NKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKG-GEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
LS N+ ++ T++P +YQYH+ + P +++R +R ++ DL + + AFDG L++P+ L+ K T + + V + S C YN++F+R+ + + +IGR+Y+ P I + R+ IWPG+ TSI ++ ++L DVSHKV+R+++V D M ++T +K Q Q + +L+G +VLT +NN+ YR+ DIDW +N S F K G ++S+ +Y++ Y +I DL+QP++VS+ R RGP G+ G LIPELC LTGLTD M DF +M++L H R+TP++R + +D I+ + ++ L WG++ + + ++ + + E I+ G + + A++ + +I ++NW+LI + AN L+Q L +P +G+ K ++E++ DRTE YL+ L++ V Q+VV +LS+ R+D+Y+AIKK+ DCP PSQ V+A+TL + + ++A IALQ+N K+GGELW V IP+K M+VG+D +HD RS+ V S N TRWFS+ Q++ +E++ L+ C+ + L+A+ N ++P I +YRDGV DGQL + E+ QF +S Y P + VV+KR+NTR + ++ G++ NP PGT++D VTR YDFF+VSQ+VR G+V+PTHY V+ DNS P +Q + YK CHIYYNWPG +RVPAPC YAHKLA++VGQ++ EP+ L +RL+YL
Sbjct: 114 LSTNHFRL-TSRPQWALYQYHIDYNPLMEARRLRSALL--FQHEDL--IGKCHAFDGTILFLPKRLQQKVTEVFSKTRNGEDVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPNDPIDIPSHRLVIWPGFTTSILQYENSIMLCTDVSHKVLRSETVLDFMFNFYHQTEEHKFQEQVS--KELIGLVVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRR----RGPGGTLP---GPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRKAIMIEVD-DRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVGIDCYHDMTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFA---QSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDNSGLKPDHIQRLTYKLCHIYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL 861
BLAST of SMEDWI-3 vs. Ensembl Human
Match: PIWIL2 (piwi like RNA-mediated gene silencing 2 [Source:HGNC Symbol;Acc:HGNC:17644]) HSP 1 Score: 556.214 bits (1432), Expect = 0.000e+0 Identity = 301/766 (39.30%), Postives = 443/766 (57.83%), Query Frame = 1
Query: 733 TLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD-KGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNV-APG-VQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
+L N VK++ +YQYHV+F+P ++ + MR G+ + V AFDG LY+P L+ + K + + S+ C YNV+F+R+ L M +GR+++ P +VL + R++IWPGY SI+ DGGL L DVSHKVIRND V D+M I + NK Q L+G IV+T +NNR YRI D+DW K F G++I++ +Y+ YG+ +++ +QP+++ + + R N G + G + L+PEL +TG+ + M KDFR M++L + ++PK+ H AL + I E A L WG+ ++KD I + E I N + +++ E K+ + + I I + W L PK + +A L+ L + +G+ P VE+ +DR E Y++ ++ + A G +Q+VV I+ R+D Y AIKK C V PVPSQVV +T+ + +L SVA I LQIN KLGGELW V IP+K+ MV+G+DV+HD RSV V S N T+W+S+ Q +EI+ L+ C++ LK ++E N+ LPE I +YRDGVSDGQL V EI Q K FE+F +Y+P MV VVQK+I+T LY++ P+N P PGT+VDHT+T DF+L++ VRQG PTHYV + + + +P +Q + +K CH+Y+NWPGT+RVPAPC YAHKLA++ G L EP+ LC+ LF+L
Sbjct: 225 SLGLNLVKIQCHNEAVYQYHVTFSPNVECKSMRF----GMLKDHQAVTGNVTAFDGSILYLPVKLQQVLELKSQRKTDSAEISIKIQMTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSAMVLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDCVLDVMHAIYQ--QNKEHFQDECTKLLVGNIVITRYNNRTYRIDDVDWNKTPKDSFTMSDGKEITFLEYYSKNYGITVKEEDQPLLIHRPSERQ-DNHG-----MLLKGEILLLPELSFMTGIPEKMKKDFRAMKDLAQQINLSPKQHHSALECLLQRIAKNEAATNELMRWGLRLQKDVHKIEGRVLPMERINLKNTSFITSQELNWVKE-VTRDPSILTIPMHFWALFYPKRAMDQARELVNMLEKIAGPIGMRMSPPAWVELKDDRIETYVRTIQSTLGAEGKIQMVVCIIMGPRDDLYGAIKKLCCVQSPVPSQVVNVRTIGQPTRLRSVAQKILLQINCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIVDSLKLCLVGSLKKFYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFE-NYQPKMVVFVVQKKISTNLYLAAPQN---FVTPTPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYVCVLNTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGHILHHEPAIQLCENLFFL 973
BLAST of SMEDWI-3 vs. Ensembl Human
Match: PIWIL2 (piwi like RNA-mediated gene silencing 2 [Source:HGNC Symbol;Acc:HGNC:17644]) HSP 1 Score: 556.214 bits (1432), Expect = 0.000e+0 Identity = 301/766 (39.30%), Postives = 443/766 (57.83%), Query Frame = 1
Query: 733 TLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD-KGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNV-APG-VQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
+L N VK++ +YQYHV+F+P ++ + MR G+ + V AFDG LY+P L+ + K + + S+ C YNV+F+R+ L M +GR+++ P +VL + R++IWPGY SI+ DGGL L DVSHKVIRND V D+M I + NK Q L+G IV+T +NNR YRI D+DW K F G++I++ +Y+ YG+ +++ +QP+++ + + R N G + G + L+PEL +TG+ + M KDFR M++L + ++PK+ H AL + I E A L WG+ ++KD I + E I N + +++ E K+ + + I I + W L PK + +A L+ L + +G+ P VE+ +DR E Y++ ++ + A G +Q+VV I+ R+D Y AIKK C V PVPSQVV +T+ + +L SVA I LQIN KLGGELW V IP+K+ MV+G+DV+HD RSV V S N T+W+S+ Q +EI+ L+ C++ LK ++E N+ LPE I +YRDGVSDGQL V EI Q K FE+F +Y+P MV VVQK+I+T LY++ P+N P PGT+VDHT+T DF+L++ VRQG PTHYV + + + +P +Q + +K CH+Y+NWPGT+RVPAPC YAHKLA++ G L EP+ LC+ LF+L
Sbjct: 225 SLGLNLVKIQCHNEAVYQYHVTFSPNVECKSMRF----GMLKDHQAVTGNVTAFDGSILYLPVKLQQVLELKSQRKTDSAEISIKIQMTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSAMVLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDCVLDVMHAIYQ--QNKEHFQDECTKLLVGNIVITRYNNRTYRIDDVDWNKTPKDSFTMSDGKEITFLEYYSKNYGITVKEEDQPLLIHRPSERQ-DNHG-----MLLKGEILLLPELSFMTGIPEKMKKDFRAMKDLAQQINLSPKQHHSALECLLQRIAKNEAATNELMRWGLRLQKDVHKIEGRVLPMERINLKNTSFITSQELNWVKE-VTRDPSILTIPMHFWALFYPKRAMDQARELVNMLEKIAGPIGMRMSPPAWVELKDDRIETYVRTIQSTLGAEGKIQMVVCIIMGPRDDLYGAIKKLCCVQSPVPSQVVNVRTIGQPTRLRSVAQKILLQINCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIVDSLKLCLVGSLKKFYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFE-NYQPKMVVFVVQKKISTNLYLAAPQN---FVTPTPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYVCVLNTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGHILHHEPAIQLCENLFFL 973
BLAST of SMEDWI-3 vs. Ensembl Human
Match: PIWIL1 (piwi like RNA-mediated gene silencing 1 [Source:HGNC Symbol;Acc:HGNC:9007]) HSP 1 Score: 516.153 bits (1328), Expect = 2.913e-168 Identity = 272/728 (37.36%), Postives = 434/728 (59.62%), Query Frame = 1
Query: 736 LSANYVKVRTTQPH--IYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXS-ECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEI-NKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKG-GEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWP 2904
LS N+ ++ T++P +YQYH+ + P +++R +R ++ DL + + AFDG L++P+ L+ K T + + V + S C YN++F+R+ + + +IGR+Y+ P I + R+ IWPG+ TSI ++ ++L DVSHKV+R+++V D M ++T +K Q Q + +L+G +VLT +NN+ YR+ DIDW +N S F K G ++S+ +Y++ Y +I DL+QP++VS+ R RGP G+ G LIPELC LTGLTD M DF +M++L H R+TP++R + +D I+ + ++ L WG++ + + ++ + + E I+ G + + A++ + +I ++NW+LI + AN L+Q L +P +G+ K ++E++ DRTE YL+ L++ V Q+VV +LS+ R+D+Y+AIKK+ DCP PSQ V+A+TL + + ++A IALQ+N K+GGELW V IP+K M+VG+D +HD RS+ V S N TRWFS+ Q++ +E++ L+ C+ + L+A+ N ++P I +YRDGV DGQL + E+ QF +S Y P + VV+KR+NTR + ++ G++ NP PGT++D VTR YDFF+VSQ+VR G+V+PTHY V+ DNS P +Q + YK CHIYYNWP
Sbjct: 114 LSTNHFRL-TSRPQWALYQYHIDYNPLMEARRLRSALL--FQHEDL--IGKCHAFDGTILFLPKRLQQKVTEVFSKTRNGEDVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPNDPIDIPSHRLVIWPGFTTSILQYENSIMLCTDVSHKVLRSETVLDFMFNFYHQTEEHKFQEQVS--KELIGLVVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRR----RGPGGTLP---GPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRKAIMIEVD-DRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVGIDCYHDMTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFA---QSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDNSGLKPDHIQRLTYKLCHIYYNWP 823
BLAST of SMEDWI-3 vs. Ensembl Celegans
Match: prg-1 (Piwi-like protein [Source:UniProtKB/TrEMBL;Acc:P90786]) HSP 1 Score: 430.639 bits (1106), Expect = 4.514e-136 Identity = 257/790 (32.53%), Postives = 440/790 (55.70%), Query Frame = 1
Query: 742 ANYVKVRTTQPH--IYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETI-IXXXXXXXXXXXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYF-YPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLG-QIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLL-QALMSKSPRVG----VMFGKPKIVEMNNDRTEEYLKELKRNV----APGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLK-------EGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENN-FLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFS---------ADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
AN++ + TQ IYQYHV F P +DS+ R K+++ + +D + FDGM LY+ + + + I ++ + K V+ + ++N + +R ++L++ ++GR+YF + + + V D M I PGY T+I+ ++ +L + K++R +S+Y + + ++ N Q +N++ G ++T +NN+ +R T +D++ + LSEF K G+ I+ ++YFKN YG++I +QP+I+S+ GK + P +V Y++PELC TGLTD+M KDF++M+E+ +H R++P++R + + ++ E+ + YWGI++ +D N+ A + E + G+QAE+ + + + G + NWI+I P S +GLL Q + ++ R+G V G+P V + +YL+ +K + + ++V +L+ + RY+++KKF V+CP+P+Q V +TL E L S+ + I LQ+ K GG LW V IP+K M+VG D++HD+ + ++VGA V +T+ T+++S++ E ++ L + LK Y++ N+ LP + +YRDG DGQ+ Y++ TE++ ++ + K + + +V KR+N R+ IN P PGT+VD TVTR DF+LV Q V QGTVTP Y ++ D++ P + Q +A+K CH+YYNW GTVRVPAPC YAHKLA++ Q+L + + L D+LF+L
Sbjct: 54 ANFIPIEMTQSDYSIYQYHVEFEPTVDSKANREKMLRDNNVTD--EIGHHFVFDGMILYLKEEWEQNQMIEVQHPIDRSLICIRFKQTNRFLVDDPQTINIFNTIIRRSFDALQLTQLGRNYFNWGDSRAVPDYN-MSILPGYETAIRMYEENFMLCVENRFKMVREESMYILFHKELRSCQNNPQRVQEKMNEMYGGTTIITRYNNKLHRYTRLDYSISPLSEFVKDGQSITLKEYFKNQYGIEITVDDQPIIISE-----GKPKQPGEPPQVS----YIVPELCFPTGLTDEMRKDFKMMKEIAKHTRMSPQQRLVESRKLIVDLSKNEKVMECFKYWGISLGQDLANVQARVLKSEPL---QGKKTYEGKQAEWARGVKECGIYRGSNMTNWIVIGPGS---GNSGLLSQKFIEEARRLGKILQVQLGEPMCVPIRGISPNDYLEGVKGAIKQVDGEDIHMLVVMLADDNKTRYDSLKKFLCVECPIPNQCVNLRTLAGKSKDGGENKNLGSIVLKIVLQMICKTGGALWKVNIPLKNTMIVGYDLYHDSTLKGKTVGACVSTTSNDFTQFYSQTRPHENPTQLGNNLTHFVRKALKQYYDSNDQTLPSRLILYRDGAGDGQIPYIKNTEVKLVRDACDAVTDKAAELSNKVQEKIKLAFIIVTKRVNMRILKQGSSLDNAIN-PQPGTVVDTTVTRPERMDFYLVPQFVNQGTVTPVSYNIIHDDTDLGPDKHQQLAFKLCHLYYNWQGTVRVPAPCQYAHKLAFLTAQSLHDDANGCLRDKLFFL 824
BLAST of SMEDWI-3 vs. Ensembl Celegans
Match: alg-2 (pep chromosome:WBcel235:II:874160:879001:1 gene:WBGene00000106.1 transcript:T07D3.7b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:alg-2) HSP 1 Score: 168.703 bits (426), Expect = 1.485e-42 Identity = 166/685 (24.23%), Postives = 298/685 (43.50%), Query Frame = 1
Query: 1066 SLRMVKIGRDYFYP------------EKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVI-RNDSVYDIMMEI----------NKTVNNKGQMQ-TAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD---KGGEKI--SYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHE-----HCRVTPKKRHEALLEFVDNIYSCE-EAKKLLGYWGITIEKDTVNINACKMN-PEMIYFG-NEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKI-------VEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQ-TLKEGPKLNSVAVNIALQINAKLGGELWAVKIPI-------KKFMVVGLDVWHDTKGRSR--SVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNK-GQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAY 2955
SL+ +GR +F P E K+ R E+W G+ S++ ++L DVS R V + + E+ + +++ +++ T I L +I R YR+ ++ F + G+ I + YF + Y +Q++ P + + YL PE+C D++ R +++L + + T + E E + E A +GITI + ++ P+++Y G + A+ + Q + + + G+ + W + L+ ++ R+ G P I ++ E K LK+ + +QL+V +L + Y +K+ + +Q V A+ ++ P+ S N+ L++N KLGG + ++ +P + + +G D+ H G +R S+ AVVGS +A +R+ + +Q+ +EI+ L + LL ++ F P I +YRDGVS+GQL V + E+ + + Y+P + + VQKR +TRL+ +D ++ G+ N PGT VD +T +DFFL S + QGT P+HY VL D++ T ++Q + Y+ CH Y +V +PAP YAH +A+
Sbjct: 191 SLKYTPVGRSFFTPPGVMKPGMQMHQESKLGGGR---EVWFGFHQSVRPSQWKMMLNIDVSATAFYRAMPVIEFVAEVLELPVQALAERRALSDAQRVKFTKEIRGLKIEITHCGAVRRKYRVCNVTRRPAQTQTFPLQLETGQTIECTVAKYFFDKYRIQLKYPHLPCLQVGQEQKH----------------TYLPPEVC-------DIVPGQRCLKKLTDVQTSTMIKATARSAPEREREICKLVSKAELSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRHRATTALPNQGVWD--MRGKQFHTGMEVRTWAIACFAQQSHVKENDLRMFTTQLQRISTDAGMPIIGTPMFCKYASGVEQVEPMFKYLKQTYS-AIQLIVVVLPG-KTPIYAEVKRVGDTVLGIATQCVQAKNAIRTTPQTLS---NLCLKMNVKLGG-VNSILLPNVRPRIFNEPVIFLGCDITHPAAGDTRKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIITDLTYMVRELLVQFYRNTRFKPARIVVYRDGVSEGQLFNVLQYELRAIREACVMLESGYQPGITFIAVQKRHHTRLFAADKADQVGKAFNIPPGTTVDVGITHPTEFDFFLCSHAGIQGTSRPSHYHVLWDDNDLTADELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVAF 841
BLAST of SMEDWI-3 vs. Ensembl Celegans
Match: alg-2 (pep chromosome:WBcel235:II:873176:878945:1 gene:WBGene00000106.1 transcript:T07D3.7a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:alg-2) HSP 1 Score: 168.318 bits (425), Expect = 1.996e-42 Identity = 166/685 (24.23%), Postives = 298/685 (43.50%), Query Frame = 1
Query: 1066 SLRMVKIGRDYFYP------------EKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVI-RNDSVYDIMMEI----------NKTVNNKGQMQ-TAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD---KGGEKI--SYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHE-----HCRVTPKKRHEALLEFVDNIYSCE-EAKKLLGYWGITIEKDTVNINACKMN-PEMIYFG-NEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKI-------VEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQ-TLKEGPKLNSVAVNIALQINAKLGGELWAVKIPI-------KKFMVVGLDVWHDTKGRSR--SVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNK-GQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAY 2955
SL+ +GR +F P E K+ R E+W G+ S++ ++L DVS R V + + E+ + +++ +++ T I L +I R YR+ ++ F + G+ I + YF + Y +Q++ P + + YL PE+C D++ R +++L + + T + E E + E A +GITI + ++ P+++Y G + A+ + Q + + + G+ + W + L+ ++ R+ G P I ++ E K LK+ + +QL+V +L + Y +K+ + +Q V A+ ++ P+ S N+ L++N KLGG + ++ +P + + +G D+ H G +R S+ AVVGS +A +R+ + +Q+ +EI+ L + LL ++ F P I +YRDGVS+GQL V + E+ + + Y+P + + VQKR +TRL+ +D ++ G+ N PGT VD +T +DFFL S + QGT P+HY VL D++ T ++Q + Y+ CH Y +V +PAP YAH +A+
Sbjct: 210 SLKYTPVGRSFFTPPGVMKPGMQMHQESKLGGGR---EVWFGFHQSVRPSQWKMMLNIDVSATAFYRAMPVIEFVAEVLELPVQALAERRALSDAQRVKFTKEIRGLKIEITHCGAVRRKYRVCNVTRRPAQTQTFPLQLETGQTIECTVAKYFFDKYRIQLKYPHLPCLQVGQEQKH----------------TYLPPEVC-------DIVPGQRCLKKLTDVQTSTMIKATARSAPEREREICKLVSKAELSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRHRATTALPNQGVWD--MRGKQFHTGMEVRTWAIACFAQQSHVKENDLRMFTTQLQRISTDAGMPIIGTPMFCKYASGVEQVEPMFKYLKQTYS-AIQLIVVVLPG-KTPIYAEVKRVGDTVLGIATQCVQAKNAIRTTPQTLS---NLCLKMNVKLGG-VNSILLPNVRPRIFNEPVIFLGCDITHPAAGDTRKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIITDLTYMVRELLVQFYRNTRFKPARIVVYRDGVSEGQLFNVLQYELRAIREACVMLESGYQPGITFIAVQKRHHTRLFAADKADQVGKAFNIPPGTTVDVGITHPTEFDFFLCSHAGIQGTSRPSHYHVLWDDNDLTADELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVAF 860
BLAST of SMEDWI-3 vs. Ensembl Celegans
Match: alg-1 (pep chromosome:WBcel235:X:13951262:13956261:1 gene:WBGene00000105.1 transcript:F48F7.1a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:alg-1) HSP 1 Score: 166.392 bits (420), Expect = 1.014e-41 Identity = 162/659 (24.58%), Postives = 289/659 (43.85%), Query Frame = 1
Query: 1099 FYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVI-RNDSVYDIMMEI----------NKTVNNKGQMQ-TAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD---KGGEKI--SYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIK--DFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEE--AKKLLGYWGITIEKDTVNINACKMN-PEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKI-------VEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQ-TLKEGPKLNSVAVNIALQINAKLGGELWAVKIPI-------KKFMVVGLDVWHDTKGRSR--SVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNK-GQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAY 2955
++ E K+ R E+W G+ S++ ++L DVS R+ V + + E+ + +++ +++ T I L +I R YR+ ++ F + G+ I + YF + Y +Q++ P + + YL PE+C + + K D + + R P++ E + N+ E A +GITI + ++ P+++Y G + + Q + + + GI + W + L+ ++ R+ G P + + ++ E K LK+N + G+QLVV +L + Y +K+ + +Q V A+ ++ P+ S N+ L++N KLGG + ++ +P + + G D+ H G SR S+ AVVGS +A +R+ + +Q+ +EI+ L + LL ++ F P I +YRDGVS+GQ V + E+ + Y+P + + VQKR +TRL+ D K++ G+ N PGT VD +T +DF+L S + QGT P+HY VL D++ T ++Q + Y+ CH Y +V +PAP YAH +A+
Sbjct: 328 YHAESKLGGGR---EVWFGFHQSVRPSQWKMMLNIDVSATAFYRSMPVIEFIAEVLELPVQALAERRALSDAQRVKFTKEIRGLKIEITHCGQMRRKYRVCNVTRRPAQTQTFPLQLETGQTIECTVAKYFYDKYRIQLKYPHLPCLQVGQEQKH----------------TYLPPEVCNIVPGQRCIKKLTDVQTSTMIKATARSAPERERE-----ISNLVRKAEFSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRTRATALPNQGVWD--MRGKQFHTGIDVRVWAIACFAQQQHVKENDLRMFTNQLQRISNDAGMPIVGNPCFCKYAVGVEQVEPMFKYLKQNYS-GIQLVVVVLPG-KTPVYAEVKRVGDTVLGIATQCVQAKNAIRTTPQTLS---NLCLKMNVKLGG-VNSILLPNVRPRIFNEPVIFFGCDITHPPAGDSRKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIISDLTYMVRELLVQFYRNTRFKPARIVVYRDGVSEGQFFNVLQYELRAIREACMMLERGYQPGITFIAVQKRHHTRLFAVDKKDQVGKAYNIPPGTTVDVGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNNLTADELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVAF 954
BLAST of SMEDWI-3 vs. Ensembl Celegans
Match: alg-1 (pep chromosome:WBcel235:X:13949114:13956261:1 gene:WBGene00000105.1 transcript:F48F7.1b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:alg-1) HSP 1 Score: 166.392 bits (420), Expect = 1.335e-41 Identity = 162/659 (24.58%), Postives = 289/659 (43.85%), Query Frame = 1
Query: 1099 FYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVI-RNDSVYDIMMEI----------NKTVNNKGQMQ-TAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD---KGGEKI--SYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIK--DFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEE--AKKLLGYWGITIEKDTVNINACKMN-PEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKI-------VEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQ-TLKEGPKLNSVAVNIALQINAKLGGELWAVKIPI-------KKFMVVGLDVWHDTKGRSR--SVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNK-GQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAY 2955
++ E K+ R E+W G+ S++ ++L DVS R+ V + + E+ + +++ +++ T I L +I R YR+ ++ F + G+ I + YF + Y +Q++ P + + YL PE+C + + K D + + R P++ E + N+ E A +GITI + ++ P+++Y G + + Q + + + GI + W + L+ ++ R+ G P + + ++ E K LK+N + G+QLVV +L + Y +K+ + +Q V A+ ++ P+ S N+ L++N KLGG + ++ +P + + G D+ H G SR S+ AVVGS +A +R+ + +Q+ +EI+ L + LL ++ F P I +YRDGVS+GQ V + E+ + Y+P + + VQKR +TRL+ D K++ G+ N PGT VD +T +DF+L S + QGT P+HY VL D++ T ++Q + Y+ CH Y +V +PAP YAH +A+
Sbjct: 349 YHAESKLGGGR---EVWFGFHQSVRPSQWKMMLNIDVSATAFYRSMPVIEFIAEVLELPVQALAERRALSDAQRVKFTKEIRGLKIEITHCGQMRRKYRVCNVTRRPAQTQTFPLQLETGQTIECTVAKYFYDKYRIQLKYPHLPCLQVGQEQKH----------------TYLPPEVCNIVPGQRCIKKLTDVQTSTMIKATARSAPERERE-----ISNLVRKAEFSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRTRATALPNQGVWD--MRGKQFHTGIDVRVWAIACFAQQQHVKENDLRMFTNQLQRISNDAGMPIVGNPCFCKYAVGVEQVEPMFKYLKQNYS-GIQLVVVVLPG-KTPVYAEVKRVGDTVLGIATQCVQAKNAIRTTPQTLS---NLCLKMNVKLGG-VNSILLPNVRPRIFNEPVIFFGCDITHPPAGDSRKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIISDLTYMVRELLVQFYRNTRFKPARIVVYRDGVSEGQFFNVLQYELRAIREACMMLERGYQPGITFIAVQKRHHTRLFAVDKKDQVGKAYNIPPGTTVDVGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNNLTADELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVAF 975
BLAST of SMEDWI-3 vs. Ensembl Fly
Match: AGO3 (gene:FBgn0250816 transcript:FBtr0299882) HSP 1 Score: 555.829 bits (1431), Expect = 0.000e+0 Identity = 301/766 (39.30%), Postives = 456/766 (59.53%), Query Frame = 1
Query: 727 SLTLSANYVKVRTTQPH-IYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVY-LIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
S+ + NY+K+ T + ++ Y V F PPIDS +RIK + + LG K FDG LY+P LL NK T+ + + + + C +YN+LF R+ L VK R F P + ++ ++E+WPGYVT++ + GGL+L CDVSH+++ +V ++++++ + N Q +A L+G IVLT +NNR Y+I DI + +N +F+ SY +Y+K + + I+D+ QP+I S K+RG +E L + LIPELC LTGL D++ D +LMRE+ RV+P +R AL +F +N+ + A+++L WG+++ ++ I+ +M+ E IYF ++ SVSAG AEF + N+++ + + WI+I ++ A LL + +G+ P ++ +++DR + Y++ L+RN+ Q+VV I R+DRY AIKK C + P+PSQV+ A+TL+ K+ SV I LQ+N KLGG LW VKIP K M+ G+D +HD R SV A V S N+ ++W+SK+ +Q + +EI+ L + LK Y + N LP I +YRDG+ DGQL EI QF V + + + Y VVQKRINTR++ + + NP PGT+VD +T++N+YDFFLVSQ VRQGTVTPTHYVVL D+ Y P +Q ++YK C +YYNW GTVR+PA CMYAHKLAY++GQ+++ + + L ++LFYL
Sbjct: 127 SVNIYCNYLKLTTDESKGVFNYEVRFFPPIDSVHLRIKYLND-HKDKLGGTK---TFDGNTLYLPILLPNKMTVFISKAEDVELQIRILYKKKEEM--RNCTQLYNILFDRVMKVLNYVKFDRKQFDPSRPKIIPLAKLEVWPGYVTAVDEYKGGLMLCCDVSHRILCQKTVLEMLVDLYQ--QNVEHYQESARKMLVGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGCTSYVEYYKQYHNINIKDVNQPLIYSIK-----KSRGIPAERE---NLQFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYF-SKISVSAGRSAEFSKHAVTNEMLKVVHLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKIRSVVQKIVLQMNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYEIPQFEMVCGN-----RIKISYIVVQKRINTRIF---SGSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRDDCNYGPDIIQKLSYKLCFLYYNWAGTVRIPACCMYAHKLAYLIGQSIQRDVAEALSEKLFYL 867
BLAST of SMEDWI-3 vs. Ensembl Fly
Match: AGO3 (gene:FBgn0250816 transcript:FBtr0299881) HSP 1 Score: 555.829 bits (1431), Expect = 0.000e+0 Identity = 301/766 (39.30%), Postives = 456/766 (59.53%), Query Frame = 1
Query: 727 SLTLSANYVKVRTTQPH-IYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVY-LIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
S+ + NY+K+ T + ++ Y V F PPIDS +RIK + + LG K FDG LY+P LL NK T+ + + + + C +YN+LF R+ L VK R F P + ++ ++E+WPGYVT++ + GGL+L CDVSH+++ +V ++++++ + N Q +A L+G IVLT +NNR Y+I DI + +N +F+ SY +Y+K + + I+D+ QP+I S K+RG +E L + LIPELC LTGL D++ D +LMRE+ RV+P +R AL +F +N+ + A+++L WG+++ ++ I+ +M+ E IYF ++ SVSAG AEF + N+++ + + WI+I ++ A LL + +G+ P ++ +++DR + Y++ L+RN+ Q+VV I R+DRY AIKK C + P+PSQV+ A+TL+ K+ SV I LQ+N KLGG LW VKIP K M+ G+D +HD R SV A V S N+ ++W+SK+ +Q + +EI+ L + LK Y + N LP I +YRDG+ DGQL EI QF V + + + Y VVQKRINTR++ + + NP PGT+VD +T++N+YDFFLVSQ VRQGTVTPTHYVVL D+ Y P +Q ++YK C +YYNW GTVR+PA CMYAHKLAY++GQ+++ + + L ++LFYL
Sbjct: 127 SVNIYCNYLKLTTDESKGVFNYEVRFFPPIDSVHLRIKYLND-HKDKLGGTK---TFDGNTLYLPILLPNKMTVFISKAEDVELQIRILYKKKEEM--RNCTQLYNILFDRVMKVLNYVKFDRKQFDPSRPKIIPLAKLEVWPGYVTAVDEYKGGLMLCCDVSHRILCQKTVLEMLVDLYQ--QNVEHYQESARKMLVGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGCTSYVEYYKQYHNINIKDVNQPLIYSIK-----KSRGIPAERE---NLQFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYF-SKISVSAGRSAEFSKHAVTNEMLKVVHLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKIRSVVQKIVLQMNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYEIPQFEMVCGN-----RIKISYIVVQKRINTRIF---SGSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRDDCNYGPDIIQKLSYKLCFLYYNWAGTVRIPACCMYAHKLAYLIGQSIQRDVAEALSEKLFYL 867
BLAST of SMEDWI-3 vs. Ensembl Fly
Match: AGO3 (gene:FBgn0250816 transcript:FBtr0299880) HSP 1 Score: 555.829 bits (1431), Expect = 0.000e+0 Identity = 301/766 (39.30%), Postives = 456/766 (59.53%), Query Frame = 1
Query: 727 SLTLSANYVKVRTTQPH-IYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVY-LIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
S+ + NY+K+ T + ++ Y V F PPIDS +RIK + + LG K FDG LY+P LL NK T+ + + + + C +YN+LF R+ L VK R F P + ++ ++E+WPGYVT++ + GGL+L CDVSH+++ +V ++++++ + N Q +A L+G IVLT +NNR Y+I DI + +N +F+ SY +Y+K + + I+D+ QP+I S K+RG +E L + LIPELC LTGL D++ D +LMRE+ RV+P +R AL +F +N+ + A+++L WG+++ ++ I+ +M+ E IYF ++ SVSAG AEF + N+++ + + WI+I ++ A LL + +G+ P ++ +++DR + Y++ L+RN+ Q+VV I R+DRY AIKK C + P+PSQV+ A+TL+ K+ SV I LQ+N KLGG LW VKIP K M+ G+D +HD R SV A V S N+ ++W+SK+ +Q + +EI+ L + LK Y + N LP I +YRDG+ DGQL EI QF V + + + Y VVQKRINTR++ + + NP PGT+VD +T++N+YDFFLVSQ VRQGTVTPTHYVVL D+ Y P +Q ++YK C +YYNW GTVR+PA CMYAHKLAY++GQ+++ + + L ++LFYL
Sbjct: 127 SVNIYCNYLKLTTDESKGVFNYEVRFFPPIDSVHLRIKYLND-HKDKLGGTK---TFDGNTLYLPILLPNKMTVFISKAEDVELQIRILYKKKEEM--RNCTQLYNILFDRVMKVLNYVKFDRKQFDPSRPKIIPLAKLEVWPGYVTAVDEYKGGLMLCCDVSHRILCQKTVLEMLVDLYQ--QNVEHYQESARKMLVGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGCTSYVEYYKQYHNINIKDVNQPLIYSIK-----KSRGIPAERE---NLQFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYF-SKISVSAGRSAEFSKHAVTNEMLKVVHLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKIRSVVQKIVLQMNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYEIPQFEMVCGN-----RIKISYIVVQKRINTRIF---SGSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRDDCNYGPDIIQKLSYKLCFLYYNWAGTVRIPACCMYAHKLAYLIGQSIQRDVAEALSEKLFYL 867
BLAST of SMEDWI-3 vs. Ensembl Fly
Match: AGO3 (gene:FBgn0250816 transcript:FBtr0301725) HSP 1 Score: 526.554 bits (1355), Expect = 4.447e-172 Identity = 288/741 (38.87%), Postives = 433/741 (58.43%), Query Frame = 1
Query: 727 SLTLSANYVKVRTTQPH-IYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVY-LIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAH 2943
S+ + NY+K+ T + ++ Y V F PPIDS +RIK + + LG K FDG LY+P LL NK T+ + + + + C +YN+LF R+ L VK R F P + ++ ++E+WPGYVT++ + GGL+L CDVSH+++ +V ++++++ + N Q +A L+G IVLT +NNR Y+I DI + +N +F+ SY +Y+K + + I+D+ QP+I S K+RG +E L + LIPELC LTGL D++ D +LMRE+ RV+P +R AL +F +N+ + A+++L WG+++ ++ I+ +M+ E IYF + SVSAG AEF + N+++ + + WI+I ++ A LL + +G+ P ++ +++DR + Y++ L+RN+ Q+VV I R+DRY AIKK C + P+PSQV+ A+TL+ K+ SV I LQ+N KLGG LW VKIP K M+ G+D +HD R SV A V S N+ ++W+SK+ +Q + +EI+ L + LK Y + N LP I +YRDG+ DGQL EI QF V + + Y VVQKRINTR++ + + NP PGT+VD +T++N+YDFFLVSQ VRQGTVTPTHYVVL D+ Y P +Q ++YK C +YYNW GTVR+PA CM +H
Sbjct: 127 SVNIYCNYLKLTTDESKGVFNYEVRFFPPIDSVHLRIKYLND-HKDKLGGTK---TFDGNTLYLPILLPNKMTVFISKAEDVELQIRILYKKKEEM--RNCTQLYNILFDRVMKVLNYVKFDRKQFDPSRPKIIPLAKLEVWPGYVTAVDEYKGGLMLCCDVSHRILCQKTVLEMLVDLYQ--QNVEHYQESARKMLVGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGCTSYVEYYKQYHNINIKDVNQPLIYSIK-----KSRGIPAERE---NLQFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYFS-KISVSAGRSAEFSKHAVTNEMLKVVHLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKIRSVVQKIVLQMNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYEIPQFEMV-----CGNRIKISYIVVQKRINTRIF---SGSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRDDCNYGPDIIQKLSYKLCFLYYNWAGTVRIPACCMVSH 842
BLAST of SMEDWI-3 vs. Ensembl Fly
Match: aub (gene:FBgn0000146 transcript:FBtr0112793) HSP 1 Score: 425.631 bits (1093), Expect = 1.799e-134 Identity = 253/783 (32.31%), Postives = 420/783 (53.64%), Query Frame = 1
Query: 730 LTLSANYVKVRTTQPH--IYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKET------IIXXXXXXXXXXXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSK---SNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQV----VLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCT-RWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEI----EQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
+T+ ANY KV +P+ IYQY V F P +++ +R + G++ FDG N++ K + ++ ++ + + +K V + +E V N++ +R L + + R Y+ P+ KI L+ RM++WPGY TSI+ + +LL ++ HKV+R +++Y+I+ + + + Q+ ++G ++LT +NN+ YRI D+D+ L +F +ISY DY+K Y + IRDL+QP+++S+ N R G ++ + +IPEL TG+TD M DFR +R + EH R+ P +R E L F + SC+++ + L W I ++ V I A + PE I FGN+ +A++ + + I W +I+P L + +Q + + + + P E+ +DR Y + + A Q+V+ ++ + E++Y+ IKK VD PVPSQV V+A ++ L S+A + +Q+NAKL G W V IP+ M VG DV H K +++S GA V + + + R+FS + + +E+ + M L++Y E++ LPE I +RDGV DGQL V +E+ ++ ++++S M + +V KRIN+R + NP PGT+VD +T YDFFLVSQ+VR GTV+PT Y V+ DN ++Q+++YK H+YYN+ GT+RVPA C YAHKLA++V +++ PS L ++L++L
Sbjct: 40 ITVQANYFKV-LKRPNWTIYQYRVDFTPDVEATRLR----RSFLYEHKGILG-GYIFDGTNMFCINQFKAVQDSPYVLELVTKSRAGENIEIKIKAVGSVQSTDAEQFQVLNLILRRAMEGLDLKLVSRYYYDPQAKINLENFRMQLWPGYQTSIRQHENDILLCSEICHKVMRTETLYNILSD---AIRDSDDYQSTFKRAVMGMVILTDYNNKTYRIDDVDFQSTPLCKFKTNDGEISYVDYYKKRYNIIIRDLKQPLVMSRPTDKNIRGGNDQA-----------IMIIPELARATGMTDAMRADFRTLRAMSEHTRLNPDRRIERLRMFNKRLKSCKQSVETLKSWNIELDSALVEIPARVLPPEKILFGNQKIFVCDARADWTNEFRTCSMFKNVHINRWYVITPSRNLRETQEFVQMCIRTASSMKMNICNPIYEEIPDDRNGTYSQAIDNAAANDPQIVMVVMRSPNEEKYSCIKKRTCVDRPVPSQVVTLKVIAPRQQKPTGLMSIATKVVIQMNAKLMGAPWQVVIPLHGLMTVGFDVCHSPKNKNKSYGAFVATMDQKESFRYFSTVNEHIKGQELSEQMSVNMACALRSYQEQHRSLPERILFFRDGVGDGQLYQVVNSEVNTLKDRLDEIYKSAGKQEGCRMTFIIVSKRINSRYFTGH-------RNPVPGTVVDDVITLPERYDFFLVSQAVRIGTVSPTSYNVISDNMGLNADKLQMLSYKMTHMYYNYSGTIRVPAVCHYAHKLAFLVAESINRAPSAGLQNQLYFL 795
BLAST of SMEDWI-3 vs. Ensembl Zebrafish
Match: piwil2 (piwi-like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:572134]) HSP 1 Score: 556.984 bits (1434), Expect = 0.000e+0 Identity = 290/781 (37.13%), Postives = 456/781 (58.39%), Query Frame = 1
Query: 688 IKEPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD-KGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVA--PGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
+KEP + + +NY+ + ++QYHV+F P ++S MR G+ + E AFDG LY+P+ L+ + K ++ + + S+ C YNV+ +R+ L + +GR+++ P ++L + R+++WPGY TSI++ DGGL L DVSHKV+RNDSV D+M I + ++ Q + +G IV+T +NNR YRI DI+W+K+ F G ++ DY++ YG+ I++L+QP+++ + RS + +V G + L+PEL +TG+ + M KDFR M++L H V ++ ++L + + NI S EA LG WG++I ++ + + E I + AS ++ + L + I + + W + P+ +A L+ + +G+ +P VE+ +DRTE ++K + + P VQLVV I++ R+D Y+AIKK C + PVPSQ + +T+ + KL SVA I LQIN KLGGELW V +P+K MV+G+DV HDT +SRSV V S N++ T+W+S+ Q ++EI+ + C+L+ L+ Y+E N+ PE I +YRDGVSDGQL V+ EI Q K FE+ +Y+P + + VVQKRI+T LY + G P+PGT++DHTVT + DF+L++ S+RQG PTHY+ + + + TP +Q + +K CH+Y+NWPGT+RVPAPC YAHKLA++ GQ L +EP+ L ++LF+L
Sbjct: 283 VKEPLQKIGTKGSPIPIGSNYIPICCKNDAVFQYHVTFTPNVESLSMRF----GMMKEHRPTTGEVVAFDGSILYLPKRLEEVVHLKAERKTDNQEIDIKIQLTKILPPSSDLCIPFYNVVLRRVMKILGLKLVGRNHYDPNAVVILGKHRLQVWPGYSTSIKHTDGGLYLVVDVSHKVLRNDSVLDVMNLIYQ--GSRESFQDECTKEFVGSIVITRYNNRTYRIDDIEWSKSPKDTFTLADGSVTTFVDYYRKNYGITIKELDQPLLIHRPKERS------RPGGKVITGEILLLPELSFMTGIPEKMRKDFRAMKDLTMHINVGAQQHTQSLKQLLHNINSNNEALSELGRWGLSISQEILVTQGRTLPSETICL-HSASFVTSPAVDWSRELVRDPSISTVPLNCWAVFYPRRATDQAEELVTTFSRVAGPMGMRVERPIRVELRDDRTETFVKSIHSQLTSEPRVQLVVCIMTGNRDDLYSAIKKLCCIQSPVPSQAINVRTISQPQKLRSVAQKILLQINCKLGGELWTVNVPLKYLMVIGVDVHHDTSKKSRSVMGFVASLNSMLTKWYSRVTFQMPNEEIINGFRVCLLAALQKYYEVNHAFPEKIVIYRDGVSDGQLKTVEHYEIPQILKCFETI-PNYEPKLAFIVVQKRISTTLYSYGSDHFG---TPSPGTVLDHTVTNRDWVDFYLMAHSIRQGCGLPTHYITVYNTANLTPDHLQRLTFKMCHLYWNWPGTIRVPAPCKYAHKLAFLSGQYLHSEPAIQLSEKLFFL 1046
BLAST of SMEDWI-3 vs. Ensembl Zebrafish
Match: piwil2 (piwi-like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:572134]) HSP 1 Score: 556.984 bits (1434), Expect = 0.000e+0 Identity = 290/781 (37.13%), Postives = 456/781 (58.39%), Query Frame = 1
Query: 688 IKEPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD-KGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVA--PGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
+KEP + + +NY+ + ++QYHV+F P ++S MR G+ + E AFDG LY+P+ L+ + K ++ + + S+ C YNV+ +R+ L + +GR+++ P ++L + R+++WPGY TSI++ DGGL L DVSHKV+RNDSV D+M I + ++ Q + +G IV+T +NNR YRI DI+W+K+ F G ++ DY++ YG+ I++L+QP+++ + RS + +V G + L+PEL +TG+ + M KDFR M++L H V ++ ++L + + NI S EA LG WG++I ++ + + E I + AS ++ + L + I + + W + P+ +A L+ + +G+ +P VE+ +DRTE ++K + + P VQLVV I++ R+D Y+AIKK C + PVPSQ + +T+ + KL SVA I LQIN KLGGELW V +P+K MV+G+DV HDT +SRSV V S N++ T+W+S+ Q ++EI+ + C+L+ L+ Y+E N+ PE I +YRDGVSDGQL V+ EI Q K FE+ +Y+P + + VVQKRI+T LY + G P+PGT++DHTVT + DF+L++ S+RQG PTHY+ + + + TP +Q + +K CH+Y+NWPGT+RVPAPC YAHKLA++ GQ L +EP+ L ++LF+L
Sbjct: 283 VKEPLQKIGTKGSPIPIGSNYIPICCKNDAVFQYHVTFTPNVESLSMRF----GMMKEHRPTTGEVVAFDGSILYLPKRLEEVVHLKAERKTDNQEIDIKIQLTKILPPSSDLCIPFYNVVLRRVMKILGLKLVGRNHYDPNAVVILGKHRLQVWPGYSTSIKHTDGGLYLVVDVSHKVLRNDSVLDVMNLIYQ--GSRESFQDECTKEFVGSIVITRYNNRTYRIDDIEWSKSPKDTFTLADGSVTTFVDYYRKNYGITIKELDQPLLIHRPKERS------RPGGKVITGEILLLPELSFMTGIPEKMRKDFRAMKDLTMHINVGAQQHTQSLKQLLHNINSNNEALSELGRWGLSISQEILVTQGRTLPSETICL-HSASFVTSPAVDWSRELVRDPSISTVPLNCWAVFYPRRATDQAEELVTTFSRVAGPMGMRVERPIRVELRDDRTETFVKSIHSQLTSEPRVQLVVCIMTGNRDDLYSAIKKLCCIQSPVPSQAINVRTISQPQKLRSVAQKILLQINCKLGGELWTVNVPLKYLMVIGVDVHHDTSKKSRSVMGFVASLNSMLTKWYSRVTFQMPNEEIINGFRVCLLAALQKYYEVNHAFPEKIVIYRDGVSDGQLKTVEHYEIPQILKCFETI-PNYEPKLAFIVVQKRISTTLYSYGSDHFG---TPSPGTVLDHTVTNRDWVDFYLMAHSIRQGCGLPTHYITVYNTANLTPDHLQRLTFKMCHLYWNWPGTIRVPAPCKYAHKLAFLSGQYLHSEPAIQLSEKLFFL 1046
BLAST of SMEDWI-3 vs. Ensembl Zebrafish
Match: piwil1 (piwi-like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:368200]) HSP 1 Score: 554.288 bits (1427), Expect = 0.000e+0 Identity = 295/770 (38.31%), Postives = 452/770 (58.70%), Query Frame = 1
Query: 718 SSPSLTLSANYVKVRTTQP--HIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
S ++ L AN++++ ++P +YQYHV + PP++SR +R ++ E+ + +A FDG L++P L+N ET++ + V + S C YN+LF+RI L M +IGR Y+ P+ + + R+ IWPG++T+I ++ ++L DVSHKV+R+++V D M + + ++ + A +L+G I+LT +NN+ YRI DI W + F KG +IS+++YFK+ YGL I D Q ++VS S G L+PE C LTGLTD M DF +M++L H R++P++R + + NI + + L WG++ E +++N + E I G A A++ + + +I + ++NW++ + A LLQ L S +G+ + ++E DR E L+ L++NVA Q+VV IL R+D+Y+ +KK+ VDCP PSQ V+++T+ + L +VA IALQ+N K+GGELW+V+IP+++ M+VG+D +HDT RS+GA+V S N +RWFSK LQ + +EI+ L+ + LKAY + NN LP I +YRDGV DG L V E+ Q + ++ DY+P + VV+KRI++R + + G+I NP PGT++D VTR YDFF+VSQ+VR G V PTHY V+ DNS P +Q + YK CH+YYNW G VRVPAPC YAHKLA++VGQ++ EP+ L D L+YL
Sbjct: 106 SGTAIELRANFMRL-LSRPMWALYQYHVDYKPPMESRRLRSALLFQHEET----LGKAHTFDGAILFLPNKLRNAETVLCSETRNGEKVEITVTLTNELPPSSPVCLQFYNILFRRILRILNMQQIGRHYYNPDDPFNIPQHRLTIWPGFMTTILQYESSIMLCSDVSHKVLRSETVLDFMYSLRQQCGDQ-RFPEACTKELVGLIILTKYNNKTYRIDDIAWDHTPNNTFKKGDTEISFKNYFKSQYGLDITDGNQVLLVSHVKRLGP-------SGRPPPGPAMLVPEFCYLTGLTDKMRADFNIMKDLASHTRLSPEQREGRINRLISNINRNGDVQNELTTWGLSFENKLLSLNGRVLPSERIIQGGRAFEYNPWTADWSKEMRGLPLISCMSLDNWLMFYTRRNADVAQSLLQTLNKVSGPMGIRMQRAVMIEYE-DRQESLLRALQQNVARETQMVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISKPQALMTVATKIALQMNCKMGGELWSVEIPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYEPKLSVVVVKKRISSRFFA---RIDGKIANPPPGTVIDTEVTRPEWYDFFIVSQAVRFGCVAPTHYNVVFDNSGLKPDHMQRLTYKLCHMYYNWQGIVRVPAPCQYAHKLAFLVGQSIHKEPNMNLDDFLYYL 858
BLAST of SMEDWI-3 vs. Ensembl Zebrafish
Match: piwil1 (piwi-like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:368200]) HSP 1 Score: 554.288 bits (1427), Expect = 0.000e+0 Identity = 295/770 (38.31%), Postives = 452/770 (58.70%), Query Frame = 1
Query: 718 SSPSLTLSANYVKVRTTQP--HIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
S ++ L AN++++ ++P +YQYHV + PP++SR +R ++ E+ + +A FDG L++P L+N ET++ + V + S C YN+LF+RI L M +IGR Y+ P+ + + R+ IWPG++T+I ++ ++L DVSHKV+R+++V D M + + ++ + A +L+G I+LT +NN+ YRI DI W + F KG +IS+++YFK+ YGL I D Q ++VS S G L+PE C LTGLTD M DF +M++L H R++P++R + + NI + + L WG++ E +++N + E I G A A++ + + +I + ++NW++ + A LLQ L S +G+ + ++E DR E L+ L++NVA Q+VV IL R+D+Y+ +KK+ VDCP PSQ V+++T+ + L +VA IALQ+N K+GGELW+V+IP+++ M+VG+D +HDT RS+GA+V S N +RWFSK LQ + +EI+ L+ + LKAY + NN LP I +YRDGV DG L V E+ Q + ++ DY+P + VV+KRI++R + + G+I NP PGT++D VTR YDFF+VSQ+VR G V PTHY V+ DNS P +Q + YK CH+YYNW G VRVPAPC YAHKLA++VGQ++ EP+ L D L+YL
Sbjct: 106 SGTAIELRANFMRL-LSRPMWALYQYHVDYKPPMESRRLRSALLFQHEET----LGKAHTFDGAILFLPNKLRNAETVLCSETRNGEKVEITVTLTNELPPSSPVCLQFYNILFRRILRILNMQQIGRHYYNPDDPFNIPQHRLTIWPGFMTTILQYESSIMLCSDVSHKVLRSETVLDFMYSLRQQCGDQ-RFPEACTKELVGLIILTKYNNKTYRIDDIAWDHTPNNTFKKGDTEISFKNYFKSQYGLDITDGNQVLLVSHVKRLGP-------SGRPPPGPAMLVPEFCYLTGLTDKMRADFNIMKDLASHTRLSPEQREGRINRLISNINRNGDVQNELTTWGLSFENKLLSLNGRVLPSERIIQGGRAFEYNPWTADWSKEMRGLPLISCMSLDNWLMFYTRRNADVAQSLLQTLNKVSGPMGIRMQRAVMIEYE-DRQESLLRALQQNVARETQMVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISKPQALMTVATKIALQMNCKMGGELWSVEIPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYEPKLSVVVVKKRISSRFFA---RIDGKIANPPPGTVIDTEVTRPEWYDFFIVSQAVRFGCVAPTHYNVVFDNSGLKPDHMQRLTYKLCHMYYNWQGIVRVPAPCQYAHKLAFLVGQSIHKEPNMNLDDFLYYL 858
BLAST of SMEDWI-3 vs. Ensembl Zebrafish
Match: piwil2 (piwi-like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:572134]) HSP 1 Score: 480.33 bits (1235), Expect = 7.698e-152 Identity = 260/729 (35.67%), Postives = 414/729 (56.79%), Query Frame = 1
Query: 688 IKEPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD-KGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVA--PGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQ 2862
+KEP + + +NY+ + ++QYHV+F P ++S MR G+ + E AFDG LY+P+ L+ + K ++ + + S+ C YNV+ +R+ L + +GR+++ P ++L + R+++WPGY TSI++ DGGL L DVSHKV+RNDSV D+M I + ++ Q + +G IV+T +NNR YRI DI+W+K+ F G ++ DY++ YG+ I++L+QP+++ + RS + +V G + L+PEL +TG+ + M KDFR M++L H V ++ ++L + + NI S EA LG WG++I ++ + + E I + AS ++ + L + I + + W + P+ +A L+ + +G+ +P VE+ +DRTE ++K + + P VQLVV I++ R+D Y+AIKK C + PVPSQ + +T+ + KL SVA I LQIN KLGGELW V +P+K MV+G+DV HDT +SRSV V S N++ T+W+S+ Q ++EI+ + C+L+ L+ Y+E N+ PE I +YRDGVSDGQL V+ EI Q K FE+ +Y+P + + VVQKRI+T LY + G P+PGT++DHTVT + DF+L++ S+RQG PTHY+ + + + TP +Q
Sbjct: 283 VKEPLQKIGTKGSPIPIGSNYIPICCKNDAVFQYHVTFTPNVESLSMRF----GMMKEHRPTTGEVVAFDGSILYLPKRLEEVVHLKAERKTDNQEIDIKIQLTKILPPSSDLCIPFYNVVLRRVMKILGLKLVGRNHYDPNAVVILGKHRLQVWPGYSTSIKHTDGGLYLVVDVSHKVLRNDSVLDVMNLIYQ--GSRESFQDECTKEFVGSIVITRYNNRTYRIDDIEWSKSPKDTFTLADGSVTTFVDYYRKNYGITIKELDQPLLIHRPKERS------RPGGKVITGEILLLPELSFMTGIPEKMRKDFRAMKDLTMHINVGAQQHTQSLKQLLHNINSNNEALSELGRWGLSISQEILVTQGRTLPSETICL-HSASFVTSPAVDWSRELVRDPSISTVPLNCWAVFYPRRATDQAEELVTTFSRVAGPMGMRVERPIRVELRDDRTETFVKSIHSQLTSEPRVQLVVCIMTGNRDDLYSAIKKLCCIQSPVPSQAINVRTISQPQKLRSVAQKILLQINCKLGGELWTVNVPLKYLMVIGVDVHHDTSKKSRSVMGFVASLNSMLTKWYSRVTFQMPNEEIINGFRVCLLAALQKYYEVNHAFPEKIVIYRDGVSDGQLKTVEHYEIPQILKCFETI-PNYEPKLAFIVVQKRISTTLYSYGSDHFG---TPSPGTVLDHTVTNRDWVDFYLMAHSIRQGCGLPTHYITVYNTANLTPDHLQ 994
BLAST of SMEDWI-3 vs. Ensembl Xenopus
Match: cd74 (CD74 molecule [Source:Xenbase;Acc:XB-GENE-922847]) HSP 1 Score: 578.17 bits (1489), Expect = 0.000e+0 Identity = 320/856 (37.38%), Postives = 489/856 (57.13%), Query Frame = 1
Query: 460 ENEEPGQIKKFVGRGRGLLNSQKECSNSTPSEVSNELKQMKISNDDKMTVSSEAKSQFENIEKPISKFRRREYPTQIKEPCNTRNDSSPSLT-LSANYVK-VRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKG-GEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
E EP + VGRGR + ++STP + +L+ + + + F ++ R+ +KE ++ SS S+ LS N++K + Q ++QYH+ +AP ++SR +R ++ E ++ +ARAFDG L++P+ L + + + + + + C YN++F+R+ + M +IGR+Y+ P +I + + I+PG+ TSI ++ ++L DVSHKV+R+++V D M +N+ V + + L+GQIVLT +NN+ YRI DI+W S F K G +IS+ DY++ Y I DL QP +V + P+G + V G + LIPE C LTGLTD M DF +M++L H R+ P++R + +F+ NI+ + +K L WG+ + + E I + + + A++ + L +I ++NW+L+ + AN L Q L S ++G+ + +VE++ D T+ Y+K L++ V P VQ+VV +LS+ R+D+Y+AIKK+ +DCPVPSQ VLA+TL + + SVA IALQ+N K+GGELW V+IP+K+ M+VG+D +HDT RS+GA V S N TRWFS+ LQ Q +EI+ L+ CM + LKA+F N LP I +YRDGV DGQL + + EI Q S DY P + VV+KR++ R + G++ NP PGTIVD +TR YDFF++SQSVR G+V+PTHY V+ D+ P +Q + YK CH+YYNWPG +RVPAPC YAHKLA++VGQ++ EP L DRL+YL
Sbjct: 37 EQAEP----EIVGRGR---QKRASVAHSTPVTQAGDLQISAGFQEISLGDRGGRRRDFHDL-----GINTRQAIEHVKE---SKTGSSGSIIQLSTNHIKLISRPQWVLFQYHIDYAPQMESRGLRCALLFQHEE----LIGKARAFDGTMLFLPKRLDKVNEVFSQTRNGETVKITITLTNELPPTSPTCFQFYNIIFRRLLKMMNMKQIGRNYYNPNDQIEISSHGLTIYPGFSTSILQYENNIMLSIDVSHKVLRSETVLDYMYNVNQKVESH-KFHDICSKDLIGQIVLTKYNNKTYRIDDINWDFTPESTFKKSDGSEISFVDYYRTQYNKGITDLNQPALVHNP-------KKPRGPQNVPAGPILLIPEFCFLTGLTDRMRSDFNVMKDLAIHTRLAPEQREIQVGKFLKNIHKDDSVQKELQDWGLNFDSKLLPFAGRVAPAEKILQAGKTADYNPQFADWSRELRGPTLIRVKHLDNWVLLYTRRNYDAANTLTQNLFKVSGQMGIKMNRAVMVEVD-DTTDAYVKVLQQKVTPDVQMVVCLLSSNRKDKYDAIKKYLCIDCPVPSQCVLAKTLNKPQTVVSVATKIALQMNCKMGGELWTVEIPLKELMIVGIDCYHDTLSGKRSIGAFVASLNPSMTRWFSRCVLQAQKQEIVDGLKVCMHAALKAWFNCNKSLPSRIIIYRDGVGDGQLKTMVEYEIPQLQDCIRSAEKDYSPKLTVIVVKKRVSARFFA---HVGGRLQNPPPGTIVDVEITRPEWYDFFIISQSVRVGSVSPTHYNVVYDSGALKPDHMQRLTYKLCHLYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPHLTLSDRLYYL 861
BLAST of SMEDWI-3 vs. Ensembl Xenopus
Match: cd74 (CD74 molecule [Source:Xenbase;Acc:XB-GENE-922847]) HSP 1 Score: 577.4 bits (1487), Expect = 0.000e+0 Identity = 320/856 (37.38%), Postives = 489/856 (57.13%), Query Frame = 1
Query: 460 ENEEPGQIKKFVGRGRGLLNSQKECSNSTPSEVSNELKQMKISNDDKMTVSSEAKSQFENIEKPISKFRRREYPTQIKEPCNTRNDSSPSLT-LSANYVK-VRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKG-GEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
E EP + VGRGR + ++STP + +L+ + + + F ++ R+ +KE ++ SS S+ LS N++K + Q ++QYH+ +AP ++SR +R ++ E ++ +ARAFDG L++P+ L + + + + + + C YN++F+R+ + M +IGR+Y+ P +I + + I+PG+ TSI ++ ++L DVSHKV+R+++V D M +N+ V + + L+GQIVLT +NN+ YRI DI+W S F K G +IS+ DY++ Y I DL QP +V + P+G + V G + LIPE C LTGLTD M DF +M++L H R+ P++R + +F+ NI+ + +K L WG+ + + E I + + + A++ + L +I ++NW+L+ + AN L Q L S ++G+ + +VE++ D T+ Y+K L++ V P VQ+VV +LS+ R+D+Y+AIKK+ +DCPVPSQ VLA+TL + + SVA IALQ+N K+GGELW V+IP+K+ M+VG+D +HDT RS+GA V S N TRWFS+ LQ Q +EI+ L+ CM + LKA+F N LP I +YRDGV DGQL + + EI Q S DY P + VV+KR++ R + G++ NP PGTIVD +TR YDFF++SQSVR G+V+PTHY V+ D+ P +Q + YK CH+YYNWPG +RVPAPC YAHKLA++VGQ++ EP L DRL+YL
Sbjct: 17 EQAEP----EIVGRGR---QKRASVAHSTPVTQAGDLQISAGFQEISLGDRGGRRRDFHDL-----GINTRQAIEHVKE---SKTGSSGSIIQLSTNHIKLISRPQWVLFQYHIDYAPQMESRGLRCALLFQHEE----LIGKARAFDGTMLFLPKRLDKVNEVFSQTRNGETVKITITLTNELPPTSPTCFQFYNIIFRRLLKMMNMKQIGRNYYNPNDQIEISSHGLTIYPGFSTSILQYENNIMLSIDVSHKVLRSETVLDYMYNVNQKVESH-KFHDICSKDLIGQIVLTKYNNKTYRIDDINWDFTPESTFKKSDGSEISFVDYYRTQYNKGITDLNQPALVHNP-------KKPRGPQNVPAGPILLIPEFCFLTGLTDRMRSDFNVMKDLAIHTRLAPEQREIQVGKFLKNIHKDDSVQKELQDWGLNFDSKLLPFAGRVAPAEKILQAGKTADYNPQFADWSRELRGPTLIRVKHLDNWVLLYTRRNYDAANTLTQNLFKVSGQMGIKMNRAVMVEVD-DTTDAYVKVLQQKVTPDVQMVVCLLSSNRKDKYDAIKKYLCIDCPVPSQCVLAKTLNKPQTVVSVATKIALQMNCKMGGELWTVEIPLKELMIVGIDCYHDTLSGKRSIGAFVASLNPSMTRWFSRCVLQAQKQEIVDGLKVCMHAALKAWFNCNKSLPSRIIIYRDGVGDGQLKTMVEYEIPQLQDCIRSAEKDYSPKLTVIVVKKRVSARFFA---HVGGRLQNPPPGTIVDVEITRPEWYDFFIISQSVRVGSVSPTHYNVVYDSGALKPDHMQRLTYKLCHLYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPHLTLSDRLYYL 841
BLAST of SMEDWI-3 vs. Ensembl Xenopus
Match: piwil3 (piwi-like RNA-mediated gene silencing 3 [Source:NCBI gene;Acc:100496286]) HSP 1 Score: 557.37 bits (1435), Expect = 0.000e+0 Identity = 294/770 (38.18%), Postives = 449/770 (58.31%), Query Frame = 1
Query: 718 SSPSLTLSANYVK-VRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKG-GEKISYRDYFKNTYGLQIRDLEQPMIVS-KSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
S ++ LS N++K V Q +YQYH+ + P ++SR +R ++ E ++ +ARAFDG L+ P+ L + + + + + + C YN++F+R+ + M +IGR+Y+ P I + + + IWPG+ TSI ++ ++L DVSHKV+R+++V D+M ++ + ++ A +LLGQIVLT +NN+ YRI +I W S F K G IS+ DY++ Y +IRDL Q +I + R G + GP + L+PELC LTGLTD M DF +MR+L H R+ P +R + + F++ I+ E +K L WG+ +K + PE I + + A++ + + +I ++NW+L+ P+ A L+Q L + ++G+ ++ E++ DR Y++ L V P Q+VV +LS++ +++Y++IKK+ + P PSQ V+A+TL + + SVA IA+Q+N K+GGELW+V+IP+ + MV+G+D +HDT RS+ V S N TRW+S+ LQ+Q +EI+ L+ C+ + L A+F N LP I +YRDGV DGQL + EI QF +S DY P + VV+KRIN R + G+I NP PGT+VD VTR YDFF+VSQSVRQGTV+PTHY V+ D+S P +Q + YK CH+YYNWPG +RVPAPC YAHKLA++VGQ++ EP L +RL+YL
Sbjct: 119 SGSTIQLSTNHIKLVSRPQWVLYQYHIDYNPQMESRRLRTALLYQHEE----LIGKARAFDGTMLFAPKRLNKATEVFSQTRNGENVKITITLTNELPPTSPTCFQFYNIIFRRLLKMMNMKQIGRNYYNPSDPIEISQHNLTIWPGFTTSILQYETSIMLSLDVSHKVLRSETVLDVMTKLYRMHGDR-DFADAVTKELLGQIVLTKYNNKTYRIDEICWNMTPQSTFKKSDGSDISFVDYYRMQYNKEIRDLRQALIANIPRRVRPGGDTGP----------IVLVPELCNLTGLTDRMRNDFGVMRDLAVHTRLPPDQREKQVARFLNYIHKDENVQKELRDWGLNFDKQLLPFVGRVSPPERILQAGRTADYNPQFADWSREMRGQTLISVRHLDNWMLLYPRRCYDAAQTLVQTLNKVAQQIGMRMNNVRMFEVD-DRANSYIQVLNAEVNPDTQMVVCVLSSIHKEKYDSIKKYLCLQIPTPSQCVVARTLSKPQTVLSVATKIAMQMNCKMGGELWSVEIPLGEMMVIGIDCYHDTLSGKRSIAGFVASMNRNLTRWYSRCVLQDQKQEIVDGLKVCLQASLHAWFNCNKNLPSRILIYRDGVGDGQLKTLVNYEIPQFLDCIKSAGRDYCPKLSIIVVKKRINARFFA---HLGGRIQNPPPGTVVDVEVTRPEWYDFFIVSQSVRQGTVSPTHYNVVYDSSGLKPDYMQRLTYKLCHLYYNWPGVIRVPAPCQYAHKLAFLVGQSIHKEPHLALSNRLYYL 869
BLAST of SMEDWI-3 vs. Ensembl Xenopus
Match: setx (senataxin [Source:Xenbase;Acc:XB-GENE-966858]) HSP 1 Score: 554.288 bits (1427), Expect = 0.000e+0 Identity = 301/781 (38.54%), Postives = 446/781 (57.11%), Query Frame = 1
Query: 694 EPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD-KGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNP-EMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPG---VQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
EP + L N +K+ +YQYHV+F P ++ R MR G+ + V AFDG LY+P L + + + + + S+ C YNV+ +R+ L + +GR+++ P VL + R+++WPGY +I+ DGGL L D++HK+IR+DSV DIM + + + Q QL+G IV+T +NNR YRI DI+W + F G K+S+ DY+ YG+ +++L+QP+++ + + R + PKG K +D +V L+PEL +TG+ + M KDFR M++L + ++PK+ H +L + + I S +AK L WG+ ++ D +++ ++ P E I N S AGE + + + + + W +I PK +A L L +G+ P VE+ +DR E Y + +K ++ G VQL+V ++S R+D Y AIKK C V PVPSQV+ +T+ + KL S+A I LQIN KLGGELW V IP+K MV+G+DV+HD RSV V S N+ T W+S+ Q ++EIM L+ C+++ L+ +FE N+ LPE I +YRDGVSDGQL V+ EI Q F++F +Y P MV VVQKR++T LY + GQ P PGT++DHTVT DFFL+S VRQG PTHY+ + + + P +Q + +K CH+Y+NWPGT+RVPAPC YAHKLA++ GQ L EPS LCD+LF+L
Sbjct: 189 EPLPKKGSKGSPCQLGLNLIKINFQNEAVYQYHVTFTPIVECRSMRF----GMMKDHRSVTGPVTAFDGSILYLPVKLAQTVELESERRTDGQKIKITIQMTKILDPSSDLCLPFYNVVMRRVFKILDLKLVGRNFYDPASSTVLQQYRLQVWPGYAANIRKTDGGLFLLVDITHKIIRSDSVLDIMNILYQ--QSPENFQDEVTKQLVGSIVITRYNNRTYRIDDIEWNMSPKDSFSMSDGSKVSFIDYYSKNYGITVKELDQPLLLHRPSER----KAPKG-KALD--IVLLLPELAFMTGIPEKMRKDFRAMKDLTQQIHLSPKQHHISLGKLLKRIESSADAKNELQRWGLFLDTD-IHMTTGRILPIEKINLRNN-SFPAGEDLNWNREVTREACRSSVHLLYWAMIYPKRASAQAQELSSMLERIGGPIGIRVNHPNCVELRDDRVETYARSIK-SLLEGEGKVQLLVCLISGTRDDLYGAIKKLCCVQNPVPSQVINTRTISQPQKLRSIAQKILLQINCKLGGELWGVDIPLKSVMVIGMDVYHDPSRGMRSVLGFVASINSCLTAWYSRVVFQLPNQEIMDSLKLCLVAALQKFFEVNHSLPEKIVVYRDGVSDGQLNTVENYEIPQLQTCFQTFD-NYNPRMVVIVVQKRVSTNLYST---ATGQFLTPQPGTVIDHTVTNRKWIDFFLMSHHVRQGCGIPTHYICVMNTANLGPDHLQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQFLHHEPSIKLCDKLFFL 949
BLAST of SMEDWI-3 vs. Ensembl Xenopus
Match: setx (senataxin [Source:Xenbase;Acc:XB-GENE-966858]) HSP 1 Score: 548.125 bits (1411), Expect = 0.000e+0 Identity = 302/791 (38.18%), Postives = 453/791 (57.27%), Query Frame = 1
Query: 691 KEPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD-KGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNP-EMIYFGNEASVSAGEQAEF---------KQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPG---VQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
+EP + L N +K+ +YQYHV+F P ++ R MR G+ + V AFDG LY+P L + + + + + S+ C YNV+ +R+ L + +GR+++ P VL + R+++WPGY +I+ DGGL L D++HK+IR+DSV DIM + + + Q QL+G IV+T +NNR YRI DI+W + F G K+S+ DY+ YG+ +++L+QP+++ + + R + PKG K +D +V L+PEL +TG+ + M KDFR M++L + ++PK+ H +L + + I S +AK L WG+ ++ D +++ ++ P E I N S AGE + + ++++N ++ + + W +I PK +A L L +G+ P VE+ +DR E Y + +K ++ G VQL+V ++S R+D Y AIKK C V PVPSQV+ +T+ + KL S+A I LQIN KLGGELW V IP+K MV+G+DV+HD RSV V S N+ T W+S+ Q ++EIM L+ C+++ L+ +FE N+ LPE I +YRDGVSDGQL V+ EI Q F++F +Y P MV VVQKR++T LY + GQ P PGT++DHTVT DFFL+S VRQG PTHY+ + + + P +Q + +K CH+Y+NWPGT+RVPAPC YAHKLA++ GQ L EPS LCD+LF+L
Sbjct: 36 REPLPKKGSKGSPCQLGLNLIKINFQNEAVYQYHVTFTPIVECRSMRF----GMMKDHRSVTGPVTAFDGSILYLPVKLAQTVELESERRTDGQKIKITIQMTKILDPSSDLCLPFYNVVMRRVFKILDLKLVGRNFYDPASSTVLQQYRLQVWPGYAANIRKTDGGLFLLVDITHKIIRSDSVLDIMNILYQ--QSPENFQDEVTKQLVGSIVITRYNNRTYRIDDIEWNMSPKDSFSMSDGSKVSFIDYYSKNYGITVKELDQPLLLHRPSER----KAPKG-KALD--IVLLLPELAFMTGIPEKMRKDFRAMKDLTQQIHLSPKQHHISLGKLLKRIESSADAKNELQRWGLFLDTD-IHMTTGRILPIEKINLRNN-SFPAGEDLNWNREVTREACRSSVSNNTIV--VHLLYWAMIYPKRASAQAQELSSMLERIGGPIGIRVNHPNCVELRDDRVETYARSIK-SLLEGEGKVQLLVCLISGTRDDLYGAIKKLCCVQNPVPSQVINTRTISQPQKLRSIAQKILLQINCKLGGELWGVDIPLKSVMVIGMDVYHDPSRGMRSVLGFVASINSCLTAWYSRVVFQLPNQEIMDSLKLCLVAALQKFFEVNHSLPEKIVVYRDGVSDGQLNTVENYEIPQLQTCFQTFD-NYNPRMVVIVVQKRVSTNLYST---ATGQFLTPQPGTVIDHTVTNRKWIDFFLMSHHVRQGCGIPTHYICVMNTANLGPDHLQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQFLHHEPSIKLCDKLFFL 804
BLAST of SMEDWI-3 vs. Ensembl Mouse
Match: Piwil1 (piwi-like RNA-mediated gene silencing 1 [Source:MGI Symbol;Acc:MGI:1928897]) HSP 1 Score: 566.614 bits (1459), Expect = 0.000e+0 Identity = 292/766 (38.12%), Postives = 465/766 (60.70%), Query Frame = 1
Query: 736 LSANYVKVRTTQPH--IYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXS-ECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEI-NKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKG-GEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
LS N+ ++ T++P +YQYH+ + P +++R +R ++ DL + AFDG L++P+ L++K T + + V + S C YN++F+R+ + + +IGR+Y+ P I + R+ IWPG+ TSI ++ ++L DVSHKV+R+++V D M + +T +K Q Q + +L+G IVLT +NN+ YR+ DIDW +N S F K G ++S+ +Y++ Y +I DL+QP++VS+ R RGP G+ G LIPELC LTGLTD M DF +M++L H R+TP++R + +D I+ + ++ L WG++ + + ++ + + E I+ G + + A++ + +I ++NW+LI + AN L+Q L +P +G+ K ++E++ DRTE YL+ L++ V Q+VV +LS+ R+D+Y+AIKK+ DCP PSQ V+A+TL + + ++A IALQ+N K+GGELW V +P+K M+VG+D +HDT RS+ V S N TRWFS+ Q++ +E++ L+ C+ + L+A+ N ++P + +YRDGV DGQL + E+ QF +S Y P + VV+KR+N R + ++ G++ NP PGT++D VTR YDFF+VSQ+VR G+V+PTHY V+ D+S P +Q + YK CH+YYNWPG +RVPAPC YAHKLA++VGQ++ EP+ L +RL+YL
Sbjct: 115 LSTNHFRL-TSRPQWALYQYHIDYNPLMEARRLRSALL--FQHEDL--IGRCHAFDGTILFLPKRLQHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYENNIMLCTDVSHKVLRSETVLDFMFNLYQQTEEHKFQEQVS--KELIGLIVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRR----RGPGGTLP---GPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWGLSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQMKKAIMIEVD-DRTEAYLRALQQKVTSDTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIVGIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYNPRLTVIVVKKRVNARFFA---QSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL 862
BLAST of SMEDWI-3 vs. Ensembl Mouse
Match: Piwil2 (piwi-like RNA-mediated gene silencing 2 [Source:MGI Symbol;Acc:MGI:1930036]) HSP 1 Score: 553.132 bits (1424), Expect = 0.000e+0 Identity = 299/766 (39.03%), Postives = 442/766 (57.70%), Query Frame = 1
Query: 733 TLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEF-DKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKR--NVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
+L N +K++ +YQYHV+F+P ++ + MR G+ + V AFDG LY+P L+ + K D + + S+ C YNV+F+R+ L M +GR+++ P +VL + R++IWPGY SI+ DGGL L DVSHKVIRNDSV D+M I + NK Q L+G IV+T +NNR YRI D+DW K F G++I++ +Y+ YG+ +++ +QP+++ + + R N G + G + L+PEL +TG+ + M KDFR M++L + ++PK+ H AL + I E A L WG+++ KD I + E I N + V++ E + + + + I I + W L PK + +A L+ L + +G+ P VE+ +DR E Y++ ++ V +Q+VV I+ R+D Y AIKK C V PVPSQV+ +T+ + +L SVA I LQ+N KLGGELW V IP+K+ MV+G+DV+HD RSV V S N T+W+S+ Q +EI+ L+ C++ LK Y+E N+ LPE I +YRDGVSDGQL V EI Q K FE+F +Y P MV VVQK+I+T LY++ P + P+PGT+VDHT+T DF+L++ VRQG PTHY+ + + + +P +Q + +K CH+Y+NWPGT+RVPAPC YAHKLA++ GQ L EP+ LC LF+L
Sbjct: 223 SLGLNLIKIQCHNEAVYQYHVTFSPSVECKSMRF----GMLKDHQSVTGNVTAFDGSILYLPVKLQQVVELKSQRKTDDAEISIKIQLTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSAMVLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDSVLDVMHAIYQ--QNKEHFQDECSKLLVGSIVITRYNNRTYRIDDVDWNKTPKDSFVMSDGKEITFLEYYSKNYGITVKEDDQPLLIHRPSERQ-NNHG-----MLLKGEILLLPELSFMTGIPEKMKKDFRAMKDLTQQINLSPKQHHGALECLLQRISQNETASNELTRWGLSLHKDVHKIEGRLLPMERINLRNTSFVTS-EDLNWVKEVTRDASILTIPMHFWALFYPKRAMDQARELVNMLEKIAGPIGMRISPPAWVELKDDRIETYIRTIQSLLGVEGKIQMVVCIIMGTRDDLYGAIKKLCCVQSPVPSQVINVRTIGQPTRLRSVAQKILLQMNCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIVDSLKLCLVGSLKKYYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFD-NYHPKMVVFVVQKKISTNLYLAAPDH---FVTPSPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYICVLNTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQILHHEPAIQLCGNLFFL 971
BLAST of SMEDWI-3 vs. Ensembl Mouse
Match: Piwil1 (piwi-like RNA-mediated gene silencing 1 [Source:MGI Symbol;Acc:MGI:1928897]) HSP 1 Score: 514.227 bits (1323), Expect = 1.283e-167 Identity = 270/731 (36.94%), Postives = 436/731 (59.64%), Query Frame = 1
Query: 736 LSANYVKVRTTQPH--IYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXS-ECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEI-NKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKG-GEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTV 2913
LS N+ ++ T++P +YQYH+ + P +++R +R ++ DL + AFDG L++P+ L++K T + + V + S C YN++F+R+ + + +IGR+Y+ P I + R+ IWPG+ TSI ++ ++L DVSHKV+R+++V D M + +T +K Q Q + +L+G IVLT +NN+ YR+ DIDW +N S F K G ++S+ +Y++ Y +I DL+QP++VS+ R RGP G+ G LIPELC LTGLTD M DF +M++L H R+TP++R + +D I+ + ++ L WG++ + + ++ + + E I+ G + + A++ + +I ++NW+LI + AN L+Q L +P +G+ K ++E++ DRTE YL+ L++ V Q+VV +LS+ R+D+Y+AIKK+ DCP PSQ V+A+TL + + ++A IALQ+N K+GGELW V +P+K M+VG+D +HDT RS+ V S N TRWFS+ Q++ +E++ L+ C+ + L+A+ N ++P + +YRDGV DGQL + E+ QF +S Y P + VV+KR+N R + ++ G++ NP PGT++D VTR YDFF+VSQ+VR G+V+PTHY V+ D+S P +Q + YK CH+YYNWP +V
Sbjct: 115 LSTNHFRL-TSRPQWALYQYHIDYNPLMEARRLRSALL--FQHEDL--IGRCHAFDGTILFLPKRLQHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYENNIMLCTDVSHKVLRSETVLDFMFNLYQQTEEHKFQEQVS--KELIGLIVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRR----RGPGGTLP---GPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWGLSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQMKKAIMIEVD-DRTEAYLRALQQKVTSDTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIVGIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYNPRLTVIVVKKRVNARFFA---QSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPVSV 827
BLAST of SMEDWI-3 vs. Ensembl Mouse
Match: Piwil4 (piwi-like RNA-mediated gene silencing 4 [Source:MGI Symbol;Acc:MGI:3041167]) HSP 1 Score: 474.167 bits (1219), Expect = 3.952e-152 Identity = 274/791 (34.64%), Postives = 438/791 (55.37%), Query Frame = 1
Query: 670 REYPTQIKEPCNTRNDSSPSLTLSANYVKVRTTQP-HIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDK-GGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVG----VMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKP-NMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
RE T +K+ C T + P + L N + Q +YQYHV+++P + SR +RI ++ S + +A+AFDG +L++ + L K T + +T+ + + S C +NV+F++I +L M +IGR+++ P + + + + ++ +WPG+ S+ +F+ LL DV++KV+RN++V D M ++ + QL+G +VLT +NN+ YRI DIDW+ F K G +++Y DY+K Y + + DL QP++VS + N P+ +V+L+PELC LTGL+ DFRLM+ + E R++P R + L VD+I A+ L WG+ +++ + E I + A A++ + + KV+ + W+++ +A L++A +S RVG G PKI++++ + +L+ ++ + P VQLV+ IL + +++ Y++IKK+ DCPVPSQ VL +TL + + SVA IA+Q+ KLGGELW+V+IP+K MVVG+D+ D ++ V V S N+ TRWFS+ LQ +I L+ CM L ++ N+ LP I +YRDGV +GQL V + E+ Q K +D + + VV+KR RL+ S + NP GT+VD TR YDF+L+SQ+ +GTV+PTHY V+ D++ P +Q + +K CH+YYNW G + VPAPC YAHKL ++V Q++ EPS L + LFYL
Sbjct: 83 REKLTHVKD-CKTGSSGIP-VRLVTNLFNLDLPQDWQLYQYHVTYSPDLASRRLRIALLYNHSI----LSDKAKAFDGASLFLSEKLDQKVTELTSETQRGETIKITLTLTSKLFPNSPVCIQFFNVIFRKILKNLSMYQIGRNFYKPSEPVEIPQYKLSLWPGFAISVSHFESKLLFNADVNYKVLRNETVLDFMTDLCLRTGMSCFTEMCH-KQLVGLVVLTRYNNKTYRIDDIDWSVKPTQAFQKRDGSEVTYVDYYKQQYDITLSDLNQPVLVSLLKRKRNDNSEPQ--------MVHLMPELCFLTGLSSQATSDFRLMKAVAEETRLSPVGRQQQLARLVDDIQRNPVARFELETWGLHFGSQ-LSLTGRVVPSEKILLQDHTCQPAFA-ADWSKDMRSCKVLSSQPLNRWLIVC----CNRAEHLIEAFLSCLRRVGGSMGFNVGYPKIIKVD-ETPAAFLRAIQVHGDPDVQLVMCILPSNQKNYYDSIKKYLSSDCPVPSQCVLTRTLNKQGTMLSVATKIAMQMTCKLGGELWSVEIPLKSLMVVGIDICRDALNKNVVVVGFVASINSRITRWFSRCVLQRTAADIADCLKVCMTGALNRWYRHNHDLPARIVVYRDGVGNGQLKAVLEYEVPQLLKSVTECGSDARSCRLSVVVVRKRCLLRLFASTDHT---VQNPPLGTVVDSEATRPEWYDFYLISQTANRGTVSPTHYNVIYDDNALKPDHMQRLTFKLCHLYYNWQGLISVPAPCQYAHKLTFLVAQSVHKEPSLELANNLFYL 848
BLAST of SMEDWI-3 vs. Ensembl Mouse
Match: Piwil4 (piwi-like RNA-mediated gene silencing 4 [Source:MGI Symbol;Acc:MGI:3041167]) HSP 1 Score: 397.512 bits (1020), Expect = 1.052e-122 Identity = 257/818 (31.42%), Postives = 412/818 (50.37%), Query Frame = 1
Query: 670 REYPTQIKEPCNTRNDSSPSLTLSANYVKVRTTQP-HIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDK-GGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIY-SCEEAKKLLGY-----WG----------------------ITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVG----VMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
RE T +K+ C T + P + L N + Q +YQYHV+++P + SR +RI ++ S + +A+AFDG +L++ + L K T + +T+ + + S C +NV+F++I +L M +IGR+++ P + + EI P Y LL DV++KV+RN++V D M ++ + QL+G +VLT +NN+ YRI DIDW+ F K G +++Y DY+K Y + + DL QP++VS + N P+ +V+L+PELC LTGL+ DFRLM+ + E R++P R + L VD+I + ++++L + W +T + I + P F + + +A ++ + + KV+ + W+++ +A L++A +S RVG G PKI++++ + +L+ ++ + P VQLV+ IL + +++ Y++IKK+ DCPVPSQ VL +TL + + SVA IA+Q+ KLGGELW+V+IP+K MVVG+D+ D ++ V V S N+ TRWFS+ LQ +I L+ CM L ++ N+ LP I +YRDGV +GQL V + E+ Q K +D + YDF+L+SQ+ +GTV+PTHY V+ D++ P +Q + +K CH+YYNW G + VPAPC YAHKL ++V Q++ EPS L + LFYL
Sbjct: 146 REKLTHVKD-CKTGSSGIP-VRLVTNLFNLDLPQDWQLYQYHVTYSPDLASRRLRIALLYNHSI----LSDKAKAFDGASLFLSEKLDQKVTELTSETQRGETIKITLTLTSKLFPNSPVCIQFFNVIFRKILKNLSMYQIGRNFYKPSEPV-------EI-PQY--------NKLLFNADVNYKVLRNETVLDFMTDLCLRTGMSCFTEMCH-KQLVGLVVLTRYNNKTYRIDDIDWSVKPTQAFQKRDGSEVTYVDYYKQQYDITLSDLNQPVLVSLLKRKRNDNSEPQ--------MVHLMPELCFLTGLSSQATSDFRLMKAVAEETRLSPVGRQQQLARLVDDIQRTLPSSQEVLSHTSLPLWAPEPGGLSSAIPLSTVLPFAQQLLTALSLSPGIPLPHLKPPSFLFLCQPAFAA----DWSKDMRSCKVLSSQPLNRWLIVC----CNRAEHLIEAFLSCLRRVGGSMGFNVGYPKIIKVD-ETPAAFLRAIQVHGDPDVQLVMCILPSNQKNYYDSIKKYLSSDCPVPSQCVLTRTLNKQGTMLSVATKIAMQMTCKLGGELWSVEIPLKSLMVVGIDICRDALNKNVVVVGFVASINSRITRWFSRCVLQRTAADIADCLKVCMTGALNRWYRHNHDLPARIVVYRDGVGNGQLKAVLEYEVPQLLKSVTECGSDAR---------------------------------------------YDFYLISQTANRGTVSPTHYNVIYDDNALKPDHMQRLTFKLCHLYYNWQGLISVPAPCQYAHKLTFLVAQSVHKEPSLELANNLFYL 878
BLAST of SMEDWI-3 vs. UniProt/SwissProt
Match: sp|A9ZSZ2|AGO3_BOMMO (Piwi-like protein Ago3 OS=Bombyx mori OX=7091 GN=AGO3 PE=1 SV=2) HSP 1 Score: 603.979 bits (1556), Expect = 0.000e+0 Identity = 330/854 (38.64%), Postives = 503/854 (58.90%), Query Frame = 1
Query: 496 GRGRGLLN----SQKECSNSTPSEVSNELKQMKISNDDKMT---VSSEAKSQFENIEKPISKFRRREYPTQIKE--PCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKG--GE--KISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
GRG LL SQK S S+V++ + +KM+ VS++A S +N +Y ++K+ P + ++ + ++ NY+ + + +++Y V F P D + +R K++ E KE + FDG LY+P L + + P D++ V+V ++F SE +YNV+FK I L++++ GR ++ I + + ++E+WPGYVT++ ++GGL+L D +H+V+R +V ++ E+ +T + + I L+G V+T +N + +R+ ID + S F+K GE +IS+ DY+K YG++I D +QP+++S+ R GS ++ LIPELC LTGLTDD +FRLM+++ + R+TP +RH A ++++++ E AK L WG++I +TVN+ A + PE +YFG+ V AE+ + + V+ + I W+L+ + A L L +G+M ++V + NDRT+ Y+ LK+ + VQLVV I S R+DRY AIKK C D PVPSQV+ A+TL K+ S+ I LQ+N KLGG LW++ IP K M+VG+D +HD R+RSV + V S N T W+SK QE+ +EI+ L+ C++ L Y N LP+ I +YRDGV DGQL +Q+ EI Q F ++Y+P + Y VVQKRINTR+++ K++ +NPNPGT+VDH +TR + YDF +VSQ V QGTVTPTHYVV+ D+S TP Q Q + YK CH+YYNWPGTVRVPAPC YAHKL+Y+VGQ + A+PS++L D+LF+L
Sbjct: 100 GRGFKLLQNLQASQKASSQIASSQVTSSAQSDIKDLTEKMSETSVSAQASSVAKN-----------KYFREVKDTPPVVKKGETGVPIEVTCNYIYLNFKENIVFEYEVKFEPDQDYKHLRFKLLNEHIEH----FKE-KTFDGTTLYVPHELPDAVRNLVSTNPYDQSKVNVSIIFRRTRRLSEMIHIYNVMFKCIMKDLKLIRFGRQHYNEHAAIQIPQHKLEVWPGYVTAVDEYEGGLMLTLDSTHRVLRTQTVLSLIKEVVQTEGANWKRKMTDI--LIGASVMTTYNKKLFRVDTIDDKMSPRSTFEKTEKGETVQISFIDYYKKNYGIEIMDWDQPLLISRDTKRM------PGSDTPTDFMICLIPELCQLTGLTDDQRSNFRLMKDVATYTRITPNQRHAAFKKYIESVMKNETAKSRLAGWGLSIAPETVNLTARTLPPETLYFGDNVRVPGKPNAEWNSEVTKHSVMQAVDIMRWVLLFTQRDKQVAMDFLSTLKRNCRPMGIMVSDAELVPLANDRTDTYVLALKKCITSSVQLVVAICSTKRDDRYAAIKKVCCADNPVPSQVINARTLMNTNKIRSITQKILLQLNCKLGGTLWSISIPFKSAMIVGIDSYHDPSRRNRSVCSFVASYNQSMTLWYSKVIFQEKGQEIVDGLKCCLVDALTHYLRSNGQLPDRIIIYRDGVGDGQLKLLQQYEIPQMKICFTILGSNYQPTLTYVVVQKRINTRIFL---KSRDGYDNPNPGTVVDHCITRRDWYDFLIVSQKVTQGTVTPTHYVVVYDDSGITPDQCQRLTYKMCHLYYNWPGTVRVPAPCQYAHKLSYLVGQCVHAQPSDVLVDKLFFL 926
BLAST of SMEDWI-3 vs. UniProt/SwissProt
Match: sp|A6N7Y9|PIWL1_CHICK (Piwi-like protein 1 OS=Gallus gallus OX=9031 GN=PIWIL1 PE=2 SV=1) HSP 1 Score: 571.237 bits (1471), Expect = 0.000e+0 Identity = 295/771 (38.26%), Postives = 457/771 (59.27%), Query Frame = 1
Query: 718 SSPSLTLSANYVKVRTTQPH--IYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXS-ECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKG-GEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
S + L AN+ ++ T++P +YQYHV + P +++R +R GL ++ + AFDG L++P+ L NK T + + V + S C YN++F+R+ + +IGR+Y+ P+ + + R+ +WPG+ +SI ++ ++L DVSHK++R+++V D M + + V + + + A +L+G IVLT +NNR YR+ DIDW N F + G +ISY DY+K Y I DL QP+++S+ + G V G V LIPELC LTGLT+ M DF +M++L H R++P++R + + VD + E +K L WG++ + ++ + E I + A++ + +I ++NW+LI + AN LLQ L +P +G+ K ++E++ DRTE YL+ L++++ P +VV ILS+ R+D+Y+AIKK+ DCP+PSQ V+A+TL + ++ IALQ+N K+GGELW+V+IP+K+ M+VG+D +HDT +S+ V S N TRWFS+ +Q + +EI+ L++C+ + L+ +F+ N +LP I +YRDGV DGQL + E+ QF ++ DY P + VV+KR++TR + + G + NP PGT+VD VTR YDFF+VSQ+VR G V PTHY V+ D SK P VQ + YK CH+YYNW G +RVPAPC YAHKLA++VGQ++ EP+ LL DRL+YL
Sbjct: 116 SGAMIKLIANFFRL-TSRPQWALYQYHVDYNPEMEARRLR----SGLLFQHEDLIGKTHAFDGSILFLPKRLPNKVTEVYSKTRNGEDVRITITLTNELPPTSPTCLQFYNIIFRRLLKMMNFQQIGRNYYNPKDPVSIPNHRLMVWPGFTSSILQYEESIMLCADVSHKILRSETVLDFMYSLYEQVEER-RFRDACAKELIGVIVLTKYNNRTYRVDDIDWDANPQCTFRRADGSEISYIDYYKRQYNQDISDLNQPVLISQYRRKRGN---------VTVGPVVLIPELCYLTGLTEKMRNDFNMMKDLAVHTRLSPEQRQREIGKLVDCMKKDECVQKELRDWGLSFDSSLLSFTGRVVQAEKILQAGNVFDYNPQFADWSRETRVAPLIHAKPLDNWLLIYTRRNYDAANMLLQNLFKVTPSMGIRMNKATMIEVD-DRTEAYLRVLQQSITPDTNIVVCILSSTRKDKYDAIKKYLCTDCPIPSQCVVARTLSKPQTALAIVTKIALQMNCKMGGELWSVEIPLKQLMIVGIDCYHDTLSGKQSIAGFVASLNEKMTRWFSRCVVQSRGQEIVDGLKACLQTALREWFKWNKYLPSRIIVYRDGVGDGQLNTLVNYEVPQFLDCLKTVGKDYNPRLTVIVVKKRVSTRFFA---QAGGGLKNPPPGTVVDIEVTRPEWYDFFIVSQAVRNGCVAPTHYNVVYDTSKLKPDHVQRLTYKLCHMYYNWSGVIRVPAPCQYAHKLAFLVGQSIHREPNLLLSDRLYYL 867
BLAST of SMEDWI-3 vs. UniProt/SwissProt
Match: sp|Q96J94|PIWL1_HUMAN (Piwi-like protein 1 OS=Homo sapiens OX=9606 GN=PIWIL1 PE=1 SV=1) HSP 1 Score: 568.54 bits (1464), Expect = 0.000e+0 Identity = 295/766 (38.51%), Postives = 465/766 (60.70%), Query Frame = 1
Query: 736 LSANYVKVRTTQPH--IYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXS-ECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEI-NKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKG-GEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
LS N+ ++ T++P +YQYH+ + P +++R +R ++ DL + + AFDG L++P+ L+ K T + + V + S C YN++F+R+ + + +IGR+Y+ P I + R+ IWPG+ TSI ++ ++L DVSHKV+R+++V D M ++T +K Q Q + +L+G +VLT +NN+ YR+ DIDW +N S F K G ++S+ +Y++ Y +I DL+QP++VS+ R RGP G+ G LIPELC LTGLTD M DF +M++L H R+TP++R + +D I+ + ++ L WG++ + + ++ + + E I+ G + + A++ + +I ++NW+LI + AN L+Q L +P +G+ K ++E++ DRTE YL+ L++ V Q+VV +LS+ R+D+Y+AIKK+ DCP PSQ V+A+TL + + ++A IALQ+N K+GGELW V IP+K M+VG+D +HD RS+ V S N TRWFS+ Q++ +E++ L+ C+ + L+A+ N ++P I +YRDGV DGQL + E+ QF +S Y P + VV+KR+NTR + ++ G++ NP PGT++D VTR YDFF+VSQ+VR G+V+PTHY V+ DNS P +Q + YK CHIYYNWPG +RVPAPC YAHKLA++VGQ++ EP+ L +RL+YL
Sbjct: 114 LSTNHFRL-TSRPQWALYQYHIDYNPLMEARRLRSALL--FQHEDL--IGKCHAFDGTILFLPKRLQQKVTEVFSKTRNGEDVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPNDPIDIPSHRLVIWPGFTTSILQYENSIMLCTDVSHKVLRSETVLDFMFNFYHQTEEHKFQEQVS--KELIGLVVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRR----RGPGGTLP---GPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRKAIMIEVD-DRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVGIDCYHDMTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFA---QSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDNSGLKPDHIQRLTYKLCHIYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL 861
BLAST of SMEDWI-3 vs. UniProt/SwissProt
Match: sp|Q9JMB7|PIWL1_MOUSE (Piwi-like protein 1 OS=Mus musculus OX=10090 GN=Piwil1 PE=1 SV=1) HSP 1 Score: 566.614 bits (1459), Expect = 0.000e+0 Identity = 292/766 (38.12%), Postives = 465/766 (60.70%), Query Frame = 1
Query: 736 LSANYVKVRTTQPH--IYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXS-ECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEI-NKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKG-GEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
LS N+ ++ T++P +YQYH+ + P +++R +R ++ DL + AFDG L++P+ L++K T + + V + S C YN++F+R+ + + +IGR+Y+ P I + R+ IWPG+ TSI ++ ++L DVSHKV+R+++V D M + +T +K Q Q + +L+G IVLT +NN+ YR+ DIDW +N S F K G ++S+ +Y++ Y +I DL+QP++VS+ R RGP G+ G LIPELC LTGLTD M DF +M++L H R+TP++R + +D I+ + ++ L WG++ + + ++ + + E I+ G + + A++ + +I ++NW+LI + AN L+Q L +P +G+ K ++E++ DRTE YL+ L++ V Q+VV +LS+ R+D+Y+AIKK+ DCP PSQ V+A+TL + + ++A IALQ+N K+GGELW V +P+K M+VG+D +HDT RS+ V S N TRWFS+ Q++ +E++ L+ C+ + L+A+ N ++P + +YRDGV DGQL + E+ QF +S Y P + VV+KR+N R + ++ G++ NP PGT++D VTR YDFF+VSQ+VR G+V+PTHY V+ D+S P +Q + YK CH+YYNWPG +RVPAPC YAHKLA++VGQ++ EP+ L +RL+YL
Sbjct: 115 LSTNHFRL-TSRPQWALYQYHIDYNPLMEARRLRSALL--FQHEDL--IGRCHAFDGTILFLPKRLQHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYENNIMLCTDVSHKVLRSETVLDFMFNLYQQTEEHKFQEQVS--KELIGLIVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRR----RGPGGTLP---GPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWGLSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQMKKAIMIEVD-DRTEAYLRALQQKVTSDTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIVGIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYNPRLTVIVVKKRVNARFFA---QSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL 862
BLAST of SMEDWI-3 vs. UniProt/SwissProt
Match: sp|Q8TC59|PIWL2_HUMAN (Piwi-like protein 2 OS=Homo sapiens OX=9606 GN=PIWIL2 PE=1 SV=1) HSP 1 Score: 556.214 bits (1432), Expect = 0.000e+0 Identity = 301/766 (39.30%), Postives = 443/766 (57.83%), Query Frame = 1
Query: 733 TLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD-KGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNV-APG-VQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
+L N VK++ +YQYHV+F+P ++ + MR G+ + V AFDG LY+P L+ + K + + S+ C YNV+F+R+ L M +GR+++ P +VL + R++IWPGY SI+ DGGL L DVSHKVIRND V D+M I + NK Q L+G IV+T +NNR YRI D+DW K F G++I++ +Y+ YG+ +++ +QP+++ + + R N G + G + L+PEL +TG+ + M KDFR M++L + ++PK+ H AL + I E A L WG+ ++KD I + E I N + +++ E K+ + + I I + W L PK + +A L+ L + +G+ P VE+ +DR E Y++ ++ + A G +Q+VV I+ R+D Y AIKK C V PVPSQVV +T+ + +L SVA I LQIN KLGGELW V IP+K+ MV+G+DV+HD RSV V S N T+W+S+ Q +EI+ L+ C++ LK ++E N+ LPE I +YRDGVSDGQL V EI Q K FE+F +Y+P MV VVQK+I+T LY++ P+N P PGT+VDHT+T DF+L++ VRQG PTHYV + + + +P +Q + +K CH+Y+NWPGT+RVPAPC YAHKLA++ G L EP+ LC+ LF+L
Sbjct: 225 SLGLNLVKIQCHNEAVYQYHVTFSPNVECKSMRF----GMLKDHQAVTGNVTAFDGSILYLPVKLQQVLELKSQRKTDSAEISIKIQMTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSAMVLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDCVLDVMHAIYQ--QNKEHFQDECTKLLVGNIVITRYNNRTYRIDDVDWNKTPKDSFTMSDGKEITFLEYYSKNYGITVKEEDQPLLIHRPSERQ-DNHG-----MLLKGEILLLPELSFMTGIPEKMKKDFRAMKDLAQQINLSPKQHHSALECLLQRIAKNEAATNELMRWGLRLQKDVHKIEGRVLPMERINLKNTSFITSQELNWVKE-VTRDPSILTIPMHFWALFYPKRAMDQARELVNMLEKIAGPIGMRMSPPAWVELKDDRIETYVRTIQSTLGAEGKIQMVVCIIMGPRDDLYGAIKKLCCVQSPVPSQVVNVRTIGQPTRLRSVAQKILLQINCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIVDSLKLCLVGSLKKFYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFE-NYQPKMVVFVVQKKISTNLYLAAPQN---FVTPTPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYVCVLNTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGHILHHEPAIQLCENLFFL 973
BLAST of SMEDWI-3 vs. TrEMBL
Match: B2Z3D6 (Piwi protein WI-3 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 1885.15 bits (4882), Expect = 0.000e+0 Identity = 958/960 (99.79%), Postives = 959/960 (99.90%), Query Frame = 1
Query: 139 LLMQKFLNKDVLVPSVESLEDKALNKLGIPLPGSTVEKNTEXXXXXXXXXXXXXXXXXXXNIKLQDSDIENRNITIVTRPLSCIGRGRGLSNPSSLTTSSGKSDKITENEEPGQIKKFVGRGRGLLNSQKECSNSTPSEVSNELKQMKISNDDKMTVSSEAKSQFENIEKPISKFRRREYPTQIKEPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
LLMQKFLNKDVLVPSVESLEDKALNKLGIP PGSTVEKNTESSSISSGDSRSNPSGDSRNNIKLQDSDIENRNITIVTRPLSCIGRGRGLSNPSSLTTSSGKSDKITENEEPGQIKKFVGRGRGLLNSQKECSNSTPSEVSNELKQMKISNDDKMTVSSEAKSQFENIEKPISKFRRREYPTQIKEPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIKVNKPTDKTVVDVKVVFTNNVNFSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYF+NTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL
Sbjct: 15 LLMQKFLNKDVLVPSVESLEDKALNKLGIPPPGSTVEKNTESSSISSGDSRSNPSGDSRNNIKLQDSDIENRNITIVTRPLSCIGRGRGLSNPSSLTTSSGKSDKITENEEPGQIKKFVGRGRGLLNSQKECSNSTPSEVSNELKQMKISNDDKMTVSSEAKSQFENIEKPISKFRRREYPTQIKEPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIKVNKPTDKTVVDVKVVFTNNVNFSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFRNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 974
BLAST of SMEDWI-3 vs. TrEMBL
Match: D2Z0E0 (DjPiwiC OS=Dugesia japonica OX=6161 GN=DjpiwiC PE=2 SV=1) HSP 1 Score: 1734.15 bits (4490), Expect = 0.000e+0 Identity = 871/962 (90.54%), Postives = 904/962 (93.97%), Query Frame = 1
Query: 139 LLMQKFLNKDVLVPSVESLEDKALNKLGIPLPGSTVEKNTEXXXXXXXXXXXXXXXXXXXNIKLQDSDIENRNITIVTRPLSCIGRGRGLSNPSSLTTSSGKSDKITENEEPGQIKKFVGRGRGLLNSQKECSNSTPSEVSNELKQMKISNDDKMTVSS-EAKSQFENIEKPI-SKFRRREYPTQIKEPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
LLMQKFLNKDV VPSVESLEDKALNKLGIP PGST EK TESSSISSGDS S +KLQDSDIEN+NITIV RPLSCIGRGRGLSNPSSL TSSG+SDKI E +E Q+KK +GRGRGLL+SQKECSNSTPSE+SNELK +K+SNDDKMTVSS E KSQFEN EKP SKFRRRE+PTQIKE CNTRND SPS+ LSANYVKV TTQPHIYQYHVSFAP IDSRLMRIKIVQGLSE LGVVKEARAFDGMNLYIPQ LKNKET+IKVNKPTD T VDVK+VFTNNVNFSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINK+VNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDW KNCLSEFDK GEKISYRDYFKNTYGLQIRDLEQP+IVSKSNSR K R KGSK+ DGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIY+CEEA KLLGYWGITIEKDTVNI ACKMNPEMIYFGNEASVSAGE AEFKQALAHN+VI GIRIENW+L++PKSL+TKANGLLQALMSKSP++GV F KP+I+EMNNDRTEEY+KELKRNV VQLVVTILSAVREDRYNAIKK CYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHD KGR RSVGAVVGSTNALCTRWFSKSHLQE DKEI+YVLQSCMLSLLKAYFEENNFLP+TIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQ+NNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNS+YTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPS+LLCDRLFYL
Sbjct: 15 LLMQKFLNKDVPVPSVESLEDKALNKLGIPPPGSTTEKTTESSSISSGDSSS--------KLKLQDSDIENKNITIVKRPLSCIGRGRGLSNPSSLATSSGQSDKIAEIDETNQVKKVIGRGRGLLSSQKECSNSTPSEISNELKDLKLSNDDKMTVSSVEDKSQFENSEKPTTSKFRRREHPTQIKEACNTRNDQSPSIILSANYVKVYTTQPHIYQYHVSFAPTIDSRLMRIKIVQGLSEDQLGVVKEARAFDGMNLYIPQQLKNKETVIKVNKPTDNTSVDVKIVFTNNVNFSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKSVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWTKNCLSEFDKSGEKISYRDYFKNTYGLQIRDLEQPLIVSKSNSRGPKGR--KGSKDADGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYNCEEANKLLGYWGITIEKDTVNIKACKMNPEMIYFGNEASVSAGENAEFKQALAHNRVISGIRIENWVLVAPKSLMTKANGLLQALMSKSPKIGVNFSKPRIIEMNNDRTEEYIKELKRNVTSSVQLVVTILSAVREDRYNAIKKHCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDAKGRGRSVGAVVGSTNALCTRWFSKSHLQEHDKEIVYVLQSCMLSLLKAYFEENNFLPDTIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQVNNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSRYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSHLLCDRLFYL 966
BLAST of SMEDWI-3 vs. TrEMBL
Match: G9B7B8 (Piwi C (Fragment) OS=Phagocata kawakatsui OX=1051007 GN=piwiC PE=2 SV=1) HSP 1 Score: 887.486 bits (2292), Expect = 0.000e+0 Identity = 414/534 (77.53%), Postives = 470/534 (88.01%), Query Frame = 1
Query: 1438 FKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGG-LVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHD------TKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
FKNTY L +RDLEQP++VSK+ +R +GPK + E + VYLIPELCMLTGLTDDM+KDFRLMRELHEHCRVTPKKRHE LL+FV+NI +CEEA +LLGYWGITI+ TVNI+ACK+ PEMI FG +A+VSAG+QA+FKQA+ HN+VIGG+RIENW++I+PKSL++KA+ LQALMSK PR+G+MF PK+VEM DRTE+Y++ELKRNV GVQLVVT+LSAVREDRYNAIKK CYVD PVPSQVVL TLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFM VGLDVWHD +KG +SVGAVVGSTNALCTRW SK Q +EI+ VLQSCM+ +LKAY+EENNFLPETIFMYRDGVSDGQLG VQ+TEIEQF K FE+FS DYKP+++YN+VQKRINTRL+V+DP++K QI NP PGT+VDHTVTR+NLYDFFLVSQSVRQGTVTPTHYVVL DNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLK EPSNL+ DRLFYL
Sbjct: 1 FKNTYNLTVRDLEQPLLVSKTKARK---QGPKPTNEKESSEFVYLIPELCMLTGLTDDMVKDFRLMRELHEHCRVTPKKRHEGLLQFVENIRNCEEATRLLGYWGITIDNGTVNIDACKLTPEMIQFGGDATVSAGDQADFKQAIGHNRVIGGMRIENWVMITPKSLVSKAHAFLQALMSKGPRIGIMFKNPKLVEMQTDRTEDYIRELKRNVTSGVQLVVTLLSAVREDRYNAIKKLCYVDSPVPSQVVLGNTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMAVGLDVWHDAAMRGRSKGAGKSVGAVVGSTNALCTRWHSKCAFQRPHQEIVDVLQSCMIGILKAYYEENNFLPETIFMYRDGVSDGQLGLVQRTEIEQFLKAFETFSPDYKPSLIYNIVQKRINTRLFVTDPRSKDQILNPGPGTVVDHTVTRSNLYDFFLVSQSVRQGTVTPTHYVVLYDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKVEPSNLMSDRLFYL 531
BLAST of SMEDWI-3 vs. TrEMBL
Match: A0A2I7YUY6 (Piwi2 OS=Eisenia fetida OX=6396 PE=2 SV=1) HSP 1 Score: 686.797 bits (1771), Expect = 0.000e+0 Identity = 363/859 (42.26%), Postives = 529/859 (61.58%), Query Frame = 1
Query: 496 GRGRGLLNSQK---------ECSNSTPS------EVSNELKQMKISNDDKMTVSSEAKSQF-ENIEKPISKFRRREYPTQIKEPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDK-GGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQI-NNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
G GR L SQK S S PS E++ L +S K+ + EA + E+P R + + P + ++ SANY+ +R +YQY VSF P +DSR MR K+ L+E V+ E +AFDG L++P LL+ +ET++K + D+ + +K+ + F+ C +YNV+F+RI + LRMV++GR+YF P + + + R+E+WPGYVT++ ++GG++LQ DVSH+V+R ++V +++ I + NN Q QL+G +VLT +NN+ YRI DI W KN LS F GE I++ DY++ Y I+D QP+++ R + PKG K ++ ++ LIPEL +TGLTD++ +D R+M++L H R+TP +R +L F N+ S EA+ L WG+ ++ D + + ++ E I N + AG +A++ +AL + VI + + NW++++ +KA + M + +G+ KP + ++ NDRTE YL+E+++ + VQ+VVTI R+DRYNAIKK C V+CPVPSQV+ A+T+ + KL SV IALQIN KLGGELWAVKIP K MV G+D +HD+ + SV V S NA CTRW+S+ Q +E+++ LQ C + L+ Y E N+ LPE I +YRDGV DGQL V E+ Q + F+ F ADY+PN+ VVQKRINTR+ I +NP PG+I+DHTVTR + +DFFLVSQ VRQGTV+PTHY+V+ D S + ++Q+++YK H+YYNWPGTVRVPAPC YAHKLAY+VGQN++ EPS LL DRLF+L
Sbjct: 147 GLGRAALLSQKYLGGTGLDAAASTSVPSGLRSVRELAAALPPPPVSGISKVEMVEEAHPESPAATEEPTKLQRMLDRLEAERAPVVRKGSLGEKVSFSANYIALRCVNAGVYQYAVSFRPAVDSRGMRFKM---LNEHK-SVIGETKAFDGAILFLPILLREQETVLKSIRQNDEQEITIKIQLVKVLPFASCRQLYNVVFRRIMSILRMVQVGRNYFLPSGAVPVPQHRLEVWPGYVTTVDEYEGGVMLQTDVSHRVLRTETVLNVISGIIR--NNPRGYQDDINKQLIGCMVLTRYNNKTYRIDDIAWDKNPLSSFASITGENITFVDYYRTAYNKVIQDHHQPLLIHIPKKRELRE-APKGKKALE--VICLIPELSFMTGLTDEIRQDHRVMKDLATHTRITPAQRRISLRNFSKNVNSVPEARAELKKWGLELDADIMELEGRILSQEKIVLRNRTT-DAGVEADWSRALTRDHVITAVDLLNWMIVTTSRDASKALEFVNMYMQCARDMGIRVEKPAVYQLQNDRTETYLREIRQKMTDQVQMVVTIFPTSRDDRYNAIKKLCCVECPVPSQVINARTISQQQKLRSVTQKIALQINCKLGGELWAVKIPTKGLMVCGVDTYHDSVQKKSSVAGFVSSVNATCTRWYSRVCCQAPGEELVHGLQVCFTASLRKYHEINHKLPERIIIYRDGVGDGQLSTVANFEVVQLMECFKHFGADYEPNLAVVVVQKRINTRILSRKGTGPNAIMDNPPPGSILDHTVTRKDWFDFFLVSQHVRQGTVSPTHYIVVHDTSNWDVDKLQMLSYKMTHLYYNWPGTVRVPAPCQYAHKLAYLVGQNIRKEPSLLLSDRLFFL 995
BLAST of SMEDWI-3 vs. TrEMBL
Match: A0A0L8HBN2 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22018299mg PE=3 SV=1) HSP 1 Score: 664.84 bits (1714), Expect = 0.000e+0 Identity = 346/890 (38.88%), Postives = 538/890 (60.45%), Query Frame = 1
Query: 379 LSCIGRGRGLSNPSSLTTSSGKSDKITENEEPGQIKKFVGRGRGLLNSQKECSNSTPSEVSNELKQMKISNDDKMTVSSEAKSQFENIEKPISKFRRREYPTQIKEPCNTRNDSSPS--------LTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGG--EKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
LS GRG L+ +SD + EP + V GRGL Q ++N K+ I ++SS A + +N + +S R+ +++ + ++D S S + LSANY++++T ++QYHVSF P IDS+ +R K++ ++ V + RAFDG LY+P L N+ET++ + +D + + + + C +YN++F+R+ + L+M ++GR Y+ P + + ++EIWPGY+T+I+ +GGLLL D SHKV+R ++V D+++ + + + + + A+ ++G +VLT +NN+ YR+ DI W + ++F G E IS+ +Y++ Y +I D QP+++ K ++ ++ G V L+ LIPE C +TGLTDDM DFR+M+++ H RVTP +R L FV++I S E+A + L WG+ E ++ I PE I E A E+A++ + L+ VI + ++NW++I+ K + K+ + +M + +G+ +PK++E + RTE ++ L+ + +QLVV I R+D+Y+A+KK C V+CPVPSQV++A+T+ + KL SV IALQIN KLGG+LWA+ IP+ MV+G+DV+HD+ RS+GA V STN TRWFS+ Q+ ++E++ L++C++ LK Y E N+ LP+ I ++RDGVSD QL V E+EQ K F+ F Y P + +VQKR+NTR++++ + Q++NP PGTIVDH++TR N YDFFLVSQ VRQGTVTPTHY+V+ D S VQ + YK H+YYNWPGT+RVPAPC YAHKLAY+VGQN+ EPS+ L DRL++L
Sbjct: 29 LSRFGRGLALTRAMEACALKEQSDSESPFPEP-LLPATVPIGRGLAAMQ---------ALTNAAKKQPIERPLGSSLSSNAPAVGKNSSQELS----RQSSVSVEDLYSPQHDLSESHRGTKGKKIPLSANYIRIKTRNEGVFQYHVSFNPEIDSKSVRYKMLYEHTD----VTGKTRAFDGAILYLPIKLPNQETVLASTRLSDGEKITITIKMVKVLKSESCIPLYNIMFRRVMSILQMKQVGRYYYNPNTPTSVPQHKLEIWPGYITAIKEHEGGLLLMADASHKVLRKETVLDVLVSLVR--QDAARFRDNAMRTIVGSVVLTRYNNKTYRVDDIAWDMSPNNKFVSSGSKESISFIEYYRKQYNREISDTNQPLLIHKPKKKALQH----GDGPVKSELICLIPEFCYMTGLTDDMRSDFRVMKDIAAHTRVTPMQRQLTLNRFVESIKSNEDAMQQLTNWGLYFEDASIQIEGRYFQPERIIM-RERDFVASEEADWGRDLSRASVITPVPLQNWVVITCKQDIGKSKDYISMMMKVTGPMGIDVSQPKLIETRDQRTETLIRLLRETIHKNLQLVVIIFPTSRDDKYSAVKKLCCVECPVPSQVIIARTISQQQKLRSVTQKIALQINCKLGGDLWALSIPLSGIMVIGIDVYHDSSKGHRSIGAFVASTNNNLTRWFSRVCFQQPNQELIDGLKTCLIGALKKYHEMNHILPKRIVVFRDGVSDSQLSVVSGYEVEQLSKCFQHFGDTYSPLLSVIIVQKRVNTRIFIN---RRNQVDNPAPGTIVDHSITRKNWYDFFLVSQHVRQGTVTPTHYIVVHDTSAMKVDHVQRLTYKMTHLYYNWPGTIRVPAPCQYAHKLAYLVGQNIHKEPSHSLADRLYFL 890
BLAST of SMEDWI-3 vs. Ensembl Cavefish
Match: piwil1 (piwi like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:103023762]) HSP 1 Score: 568.926 bits (1465), Expect = 0.000e+0 Identity = 319/855 (37.31%), Postives = 479/855 (56.02%), Query Frame = 1
Query: 472 PGQIKKFVGRGRGLLNSQKECSNSTPSE----VSNELKQMKISNDDKMTVSSEAKSQFENIEKPISKFRRREYPTQIKEPCNTRNDSSPSLTLSANYVKVRT-TQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
P + VGRGR Q+ + SE +S +Q+KI + F + + R+ +KE + S + L AN++++ + Q +YQYHV F PP++SR +R ++ E+ + +AR FDG L++P L N ET++ + V + S C YN++F+RI L M +IGR Y+ P+ + + + R+ IWPG+VT+I ++ ++L DVSHKV+R+++V D M + K ++ + A +L+G IVLT +NN+ YRI DI W + F +G IS++DY+K YGL I D Q ++VS+ S G LIPE C LTGLTD M DF +M++L H R+TP++R L F+ NI EA+ L WG++ E + + + E I G+ A++ + + +I + +E+W++ + A LLQ L S +G+ + ++E DR E L+ L++NV P Q+VV IL R+D+Y+ +KK+ VDCP PSQ V+++TL L +VA IALQ+N K+GGELW+++IP+K+ M+VG+D +HDT RS+GA+V S N +RWFSK LQ + +EI+ L+ + LKAY++ NN LP I +YRDGV DG L V E+ Q + ++ DY P + VV+KRI +R + + G++ NP PGT++D VTR YDFF+VSQ+VR G+V+PTHY V+ DNS P +Q + YK CH+YYNW G VRVPAPC YAHKLA++VGQ++ EP+ L D L+YL
Sbjct: 36 PSAEGELVGRGR-----QRRAPGAVASEALLQISAGFQQVKIGE------RGGRRRDFHD-----AGVHTRQLMEHVKE--SKTGVSGSKIELRANFMRLLSRPQWALYQYHVDFKPPMESRRLRSALLFQHDET----LGKARTFDGAILFLPHRLHNTETVLCSETRHGERVQITVTLTNELPPSSPVCLQFYNIIFRRILRMLNMQQIGRHYYNPDDPLNIPQHRLTIWPGFVTNILQYESSIMLCTDVSHKVLRSETVLDFMYNLRKQCGDQ-RFPDACTKELVGLIVLTKYNNKTYRIDDIAWDHTPNNTFKRGDADISFKDYYKTQYGLDITDGNQVLLVSEVKRMGP-------SGAPPPGPALLIPEFCYLTGLTDKMRNDFNIMKDLSSHTRLTPEQRESRLNRFITNISRNTEAQNELSTWGLSFENKLLGLTGRVLPAERILQGSRTYDYNPWAADWSKEMRGLPLISSMPLESWLMFYTRRNSDVAQSLLQTLNRVSGPMGIRMQRAVMIEYE-DRQESLLRALQQNVGPDTQMVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTLSRPQALMTVATKIALQMNCKMGGELWSIEIPLKQLMIVGIDCYHDTAAGKRSIGALVASLNHGMSRWFSKCVLQSRGQEIIDGLKGALKGALKAYWKYNNCLPSRIIVYRDGVGDGMLQSVVNYEVPQIMQSIKTMGEDYMPKLTVVVVKKRIASRFFA---RIDGKLTNPPPGTVIDTEVTRPEWYDFFIVSQAVRCGSVSPTHYNVVFDNSGLKPDHMQRLTYKLCHMYYNWQGIVRVPAPCQYAHKLAFLVGQSIHKEPNINLDDFLYYL 856
BLAST of SMEDWI-3 vs. Ensembl Cavefish
Match: piwil2 (piwi like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:103024883]) HSP 1 Score: 555.058 bits (1429), Expect = 3.217e-180 Identity = 293/782 (37.47%), Postives = 450/782 (57.54%), Query Frame = 1
Query: 691 KEPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPM---VYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD-KGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVA--PGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
+EP S+T+ ANY+ +R ++QYHV+F P +++ MR G+ + E AFDG LY+P+ L E + + + V C + YNV+ +R+ + + +GRD++ P IVL + R+++WPGY T I+ DGGL L DVSHKV+RNDSV +IM I + + Q + +G IV+T +NNR YRI DI W+K+ F G + ++ DY++ YG+ +++++QP++V + RS G K++ G + L+PEL +TG+ + M KDFR M++L H V+ ++ ++ + + NI S EA L WG+ I + + + E I AS + A++ + + + I I + W + P+ +A L+ + +G+ +P VE+ +DRTE Y+K ++ + P VQLVV I++ R+D Y+AIKK C V P PSQ + +T+ + KL S+A I LQ+N KLGGELW V +P+K MV+G+DV HD + RSV V S N+L TRW+S+ Q ++EI+ + C+L+ L+ Y+E N+ PE I +YRDGVSDGQL V++ EI Q K FE+F +Y+P + + VVQKRI+T LY + G P PGT++DHTVT + DFFL++ S+RQG PTHY+ + + + TP +Q + +K CH+Y+NWPGT+RVPAPC YAHKLA++ GQ L +EP+ L D+L++L
Sbjct: 341 REPLKKAGTKGNSITIGANYIPIRCKNEAVFQYHVTFTPNVEALSMRF----GMMKDHRSTTGEVVAFDGSILYLPKRL--DEVVHLKAERKTDNQEIDIKIQMTKVLPPHCDLCIPFYNVVLRRVMKIVGLKLVGRDHYDPSNAIVLGKHRLQVWPGYSTCIKETDGGLYLGVDVSHKVLRNDSVLNIMGVIYQ--QSPEHFQDECTKEFVGAIVITRYNNRTYRIDDIKWSKSPKDSFTLADGSETTFIDYYRKNYGITVKEIDQPLLVHRPKERSK-----PGGKQIITGEILLLPELSFMTGIPEKMRKDFRAMKDLTVHINVSAEQHTHSIKQLLQNINSNPEALNELQRWGLEISPEILMTQGRTLPMETICL-QSASFVSSSSADWSREVVRDASISSIPLYCWAVFYPRRAAEQAEELVTMFGKVAGPIGIKLERPIRVELRDDRTETYVKSIQSQLTSEPNVQLVVCIITGNRDDLYSAIKKLCCVQSPCPSQAINVRTISQPQKLRSIAQKILLQMNCKLGGELWTVSVPLKHLMVIGVDVHHDVSKKKRSVMGFVASLNSLLTRWYSRVTFQMPNEEIINGFRVCLLAALQKYYEMNHSFPEKIVIYRDGVSDGQLKTVEQYEIPQILKCFETF-PNYEPKLTFIVVQKRISTTLYSYGGERFG---TPPPGTVLDHTVTNKDWVDFFLMAHSIRQGCGLPTHYISVYNTANLTPDHLQRLTFKMCHLYWNWPGTIRVPAPCKYAHKLAFLSGQYLHSEPAIQLADKLYFL 1104
BLAST of SMEDWI-3 vs. Ensembl Cavefish
Match: piwil2 (piwi like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:103024883]) HSP 1 Score: 554.673 bits (1428), Expect = 1.214e-179 Identity = 293/782 (37.47%), Postives = 450/782 (57.54%), Query Frame = 1
Query: 691 KEPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPM---VYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD-KGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVA--PGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
+EP S+T+ ANY+ +R ++QYHV+F P +++ MR G+ + E AFDG LY+P+ L E + + + V C + YNV+ +R+ + + +GRD++ P IVL + R+++WPGY T I+ DGGL L DVSHKV+RNDSV +IM I + + Q + +G IV+T +NNR YRI DI W+K+ F G + ++ DY++ YG+ +++++QP++V + RS G K++ G + L+PEL +TG+ + M KDFR M++L H V+ ++ ++ + + NI S EA L WG+ I + + + E I AS + A++ + + + I I + W + P+ +A L+ + +G+ +P VE+ +DRTE Y+K ++ + P VQLVV I++ R+D Y+AIKK C V P PSQ + +T+ + KL S+A I LQ+N KLGGELW V +P+K MV+G+DV HD + RSV V S N+L TRW+S+ Q ++EI+ + C+L+ L+ Y+E N+ PE I +YRDGVSDGQL V++ EI Q K FE+F +Y+P + + VVQKRI+T LY + G P PGT++DHTVT + DFFL++ S+RQG PTHY+ + + + TP +Q + +K CH+Y+NWPGT+RVPAPC YAHKLA++ GQ L +EP+ L D+L++L
Sbjct: 371 REPLKKAGTKGNSITIGANYIPIRCKNEAVFQYHVTFTPNVEALSMRF----GMMKDHRSTTGEVVAFDGSILYLPKRL--DEVVHLKAERKTDNQEIDIKIQMTKVLPPHCDLCIPFYNVVLRRVMKIVGLKLVGRDHYDPSNAIVLGKHRLQVWPGYSTCIKETDGGLYLGVDVSHKVLRNDSVLNIMGVIYQ--QSPEHFQDECTKEFVGAIVITRYNNRTYRIDDIKWSKSPKDSFTLADGSETTFIDYYRKNYGITVKEIDQPLLVHRPKERSK-----PGGKQIITGEILLLPELSFMTGIPEKMRKDFRAMKDLTVHINVSAEQHTHSIKQLLQNINSNPEALNELQRWGLEISPEILMTQGRTLPMETICL-QSASFVSSSSADWSREVVRDASISSIPLYCWAVFYPRRAAEQAEELVTMFGKVAGPIGIKLERPIRVELRDDRTETYVKSIQSQLTSEPNVQLVVCIITGNRDDLYSAIKKLCCVQSPCPSQAINVRTISQPQKLRSIAQKILLQMNCKLGGELWTVSVPLKHLMVIGVDVHHDVSKKKRSVMGFVASLNSLLTRWYSRVTFQMPNEEIINGFRVCLLAALQKYYEMNHSFPEKIVIYRDGVSDGQLKTVEQYEIPQILKCFETF-PNYEPKLTFIVVQKRISTTLYSYGGERFG---TPPPGTVLDHTVTNKDWVDFFLMAHSIRQGCGLPTHYISVYNTANLTPDHLQRLTFKMCHLYWNWPGTIRVPAPCKYAHKLAFLSGQYLHSEPAIQLADKLYFL 1134
BLAST of SMEDWI-3 vs. Ensembl Cavefish
Match: piwil2 (piwi like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:103024883]) HSP 1 Score: 513.842 bits (1322), Expect = 1.681e-164 Identity = 260/648 (40.12%), Postives = 393/648 (60.65%), Query Frame = 1
Query: 1084 IGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD-KGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVA--PGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
+GRD++ P IVL + R+++WPGY T I+ DGGL L DVSHKV+RNDSV +IM I + + Q + +G IV+T +NNR YRI DI W+K+ F G + ++ DY++ YG+ +++++QP++V + RS G K++ G + L+PEL +TG+ + M KDFR M++L H V+ ++ ++ + + NI S EA L WG+ I + + + E I AS + A++ + + + I I + W + P+ +A L+ + +G+ +P VE+ +DRTE Y+K ++ + P VQLVV I++ R+D Y+AIKK C V P PSQ + +T+ + KL S+A I LQ+N KLGGELW V +P+K MV+G+DV HD + RSV V S N+L TRW+S+ Q ++EI+ + C+L+ L+ Y+E N+ PE I +YRDGVSDGQL V++ EI Q K FE+F +Y+P + + VVQKRI+T LY + G P PGT++DHTVT + DFFL++ S+RQG PTHY+ + + + TP +Q + +K CH+Y+NWPGT+RVPAPC YAHKLA++ GQ L +EP+ L D+L++L
Sbjct: 468 VGRDHYDPSNAIVLGKHRLQVWPGYSTCIKETDGGLYLGVDVSHKVLRNDSVLNIMGVIYQ--QSPEHFQDECTKEFVGAIVITRYNNRTYRIDDIKWSKSPKDSFTLADGSETTFIDYYRKNYGITVKEIDQPLLVHRPKERSKP-----GGKQIITGEILLLPELSFMTGIPEKMRKDFRAMKDLTVHINVSAEQHTHSIKQLLQNINSNPEALNELQRWGLEISPEILMTQGRTLPMETICL-QSASFVSSSSADWSREVVRDASISSIPLYCWAVFYPRRAAEQAEELVTMFGKVAGPIGIKLERPIRVELRDDRTETYVKSIQSQLTSEPNVQLVVCIITGNRDDLYSAIKKLCCVQSPCPSQAINVRTISQPQKLRSIAQKILLQMNCKLGGELWTVSVPLKHLMVIGVDVHHDVSKKKRSVMGFVASLNSLLTRWYSRVTFQMPNEEIINGFRVCLLAALQKYYEMNHSFPEKIVIYRDGVSDGQLKTVEQYEIPQILKCFETF-PNYEPKLTFIVVQKRISTTLYSYGGERFG---TPPPGTVLDHTVTNKDWVDFFLMAHSIRQGCGLPTHYISVYNTANLTPDHLQRLTFKMCHLYWNWPGTIRVPAPCKYAHKLAFLSGQYLHSEPAIQLADKLYFL 1103
BLAST of SMEDWI-3 vs. Ensembl Cavefish
Match: ago4 (protein argonaute-4 [Source:NCBI gene;Acc:103043810]) HSP 1 Score: 175.637 bits (444), Expect = 1.119e-44 Identity = 177/693 (25.54%), Postives = 286/693 (41.27%), Query Frame = 1
Query: 1066 SLRMVKIGRDYFYPEKKIV--LDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVI-RNDSVYDIMMEINKTVNNKGQMQ----------TAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD---KGGEKI--SYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTG-------LTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAH----------NKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVE-------MNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVV-LAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKK-------FMVVGLDVWHDTKGRSR--SVGAVVGSTNALCTRWFSKSHLQ--EQD--------KEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNK-GQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAY 2955
S+R +GR +F P + L R E+W G+ S++ ++L DVS R V + M E+ N Q + T I L ++ R YR+ ++ F + G+ + + YFK Y LQ++ P + + YL E+C + LTD+ + R P R E + V + L +GI + D + + M+ +G S G + +A + GI I+ W + LL++ + ++ G P + D E K LK + G+QL+V IL + Y +K+ + +Q V + +K P+ S N+ L+INAKLGG + V +P ++ + +G DV H G + S+ AVVGS + +R+ + +Q QD +E++ L + + LL +++ F P I YR GVS+GQ+ V E+ K S DY+P + Y VVQKR +TRL+ SD + G+ N GT VD T+T + +DF+L S + QGT P+HY VL D++ +T ++QL+ Y+ CH Y +V +PAP YA +A+
Sbjct: 163 SMRYTPVGRSFFSPPEGYYHPLGGGR-EVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKH----------------TYLPLEVCNIVAGQRCIKKLTDNQTSTM-----IKATARSAPD-RQEEISRLVKSNSMVGGPDPYLKEFGIVVHNDMTEVTGRVLPAPMLQYGGRVSTDTGRDCGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMSYV-GLQLIVVILPG-KTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLS---NLCLKINAKLGG-INNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQDLSQEQLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKAERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAF 826
BLAST of SMEDWI-3 vs. Ensembl Sea Lamprey
Match: piwil2 (piwi-like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:572134]) HSP 1 Score: 573.163 bits (1476), Expect = 0.000e+0 Identity = 293/781 (37.52%), Postives = 459/781 (58.77%), Query Frame = 1
Query: 688 IKEPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEF-DKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVA--PGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
+++P L ++ NY+++R + IYQYHV+F+P +DS R GL V+ +AFDG LY+P+ L + + + S+ C +YN++FK+I L + IG+ Y P + IV++R R+ IWPGYV ++Q DGGL+L DVSH+V+R DSV ++M + ++ + Q L+G +V+T +NNR YRI DI W K S F + G+ +S+ +Y+K Y + I DL QP+++ ++ R+ + +V + LIPEL +TG+TD M DF +M+ + EH R+ P RH +L+EF+ N+ EA++ L WG+ ++ D ++ ++ PE+I +A + E A++ + + + I + ++NW ++ + + + + P +G++ P I+E+ ND T+ Y+ +++N + GVQ+V+ I +R+DRY+ +K+ C V+ VPSQVV +T+ + +L SVA + LQ+N KLGGELW + IP+K MVVG+DV+H+ +SV V S N +RW+S+ Q +E++ L CM S +K Y+E N++LPE I MYRDGVSD QL V+ E+ Q F +F Y+P M+ VVQKRI+T ++++ N G + N G+++D T +DFFLVSQ VRQGTV+PTHYVV+ ++ K + VQ + YK H+YYNW GTVRVPAPC YAHKLA +VG+NL ++P LCD+LF+L
Sbjct: 9 VRQPIQKIGTYGTPLEIATNYIRLRCSNEAIYQYHVTFSPDVDSTNTRF----GLLNEHKSVIGNTKAFDGCVLYLPRKLDDNLNLTSKRISDGVEITLSIKCTKIIKPDSDLCLPIYNIIFKKIMRLLELKLIGKSYLNPRQPIVINRYRIHIWPGYVVAVQKKDGGLMLMLDVSHRVLRMDSVLEVMTHLYESCQSSANFQEECTKALVGHVVITRYNNRTYRIDDIAWDKTPKSTFLNSAGKTMSFVEYYKTQYEITIDDLGQPLLLHRAKQRN------RTDDQVVIQTICLIPELAYMTGITDLMRSDFSMMKAISEHMRLGPSLRHNSLMEFISNVKRNPEAQQELSNWGLQMDSDIYKLSGRQLPPEIIRLQYKAFAFSPE-ADWTRHIVNTPCITAVAMKNWQVLCTHRDSFLVHEFVTMMCNVGPPMGLVHQLPNIIELPNDHTKTYIDCIRKNYSYSQGVQMVMCIFPTLRDDRYSTVKQVCCVELAVPSQVVQTRTISQQKRLRSVAQKVVLQMNCKLGGELWILDIPLKNLMVVGVDVFHNVVLGKQSVVGFVASMNRSVSRWYSRVVRQSTSQELVDGLNLCMTSAIKKYYELNHYLPEKIIMYRDGVSDNQLAIVRDFEVMQLTNGFRTFDLSYQPKMLVVVVQKRISTNIFLN---NSGAMENAPSGSVLDTVATSQEWFDFFLVSQHVRQGTVSPTHYVVIFNSLKLSADHVQRLTYKMTHLYYNWSGTVRVPAPCQYAHKLASLVGENLHSDPDPGLCDKLFFL 775
BLAST of SMEDWI-3 vs. Ensembl Sea Lamprey
Match: piwil1 (piwi-like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:368200]) HSP 1 Score: 496.893 bits (1278), Expect = 2.344e-161 Identity = 267/769 (34.72%), Postives = 448/769 (58.26%), Query Frame = 1
Query: 718 SSPSLTLSANYVKVRTTQP-HIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKG-GEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
S + L NY K+ + + +YQYHV+F+P ++S+ +RI ++ +E ++ E RAFDG LY+P+ L+ + + K + + + + +++N++F+++ + M +IGR+Y+ P+ + + R+ +WPG+VTSI ++ ++L DVSHK++RN+SV D M + + + + A +L+GQ++LT +NNR YRI DI W +N F K G +++Y +Y+ Y L+++D++QP+++S+S + + + E++ ++L+PE+ LTGL+D M DF LM++L +V P KR +F++ + S E WG++ ++ +++ + E +Y +++ QA++ + + K++ I +E W+L+ + +A ++ LM P +G+ KP++ E+ DR + +++ L++N+ Q+V+ +L + + +Y++IK+F V+ P PSQ VLA+TL + + SVA IALQ+N KLGGELW+V+IPIK+ MVVG+D +HD+ R +SV V S N TRW+S+ Q +E++ L+ CM + NF+ I + + V + I F S+ +P + VV+KR R + + + NP+PGT++D TR YDF+LVSQSVRQGTVTPTHY V+ D S P +Q +AYK CH+YYNWPG +RVPAPC YAHKLA++VGQ++ EPS L DRL+YL
Sbjct: 128 SGQPVKLLTNYFKLMSRKMWMVYQYHVTFSPELESKRLRIALLHEHNE----LLGETRAFDGCILYLPKKLEKVSEVWSKTRQEQKIKITITLTNELPPSSPTAIVLFNIIFRKVLKMVNMQQIGRNYYNPDDAFTIPQHRLHVWPGFVTSIMCYESDVMLCADVSHKILRNESVLDFMYTLFEQIEER-HFHDACQKELVGQVILTRYNNRTYRIDDISWNRNPSMTFLKSDGTEVTYYEYYTKHYSLEVKDMQQPLLISRSKRKDSVGK----TVEIE---LHLLPEISYLTGLSDKMRSDFHLMKDLGLQTQVGPDKRAMTTQKFIEKLQSNPETCNEFAKWGLSFDRQLLSLTGRVVPCEKLYQKDQSYQYNQRQADWSRESRNMKLMTPISLEEWMLLFTHNNSNEARAIVDNLMQVGPPMGMHINKPEMFEVL-DRAQSFVQTLQQNLNHHTQMVMCVLPTIHKGKYDSIKQFLCVERPFPSQCVLARTLSKPQMIRSVATKIALQMNCKLGGELWSVEIPIKQLMVVGVDSYHDSITRGQSVCGFVASLNQTLTRWYSRCIFQHAAQEMIDGLKVCM--------QCKNFISLQIASHSNDV-------MINVVIIYFLSFLNSYCCGSRPKVTVVVVKKRGIARFF---EFGRSGLQNPSPGTVIDTEATRHEWYDFYLVSQSVRQGTVTPTHYNVVYDTSNMKPDHMQRLAYKMCHLYYNWPGVIRVPAPCHYAHKLAFLVGQSIHKEPSLDLSDRLYYL 865
BLAST of SMEDWI-3 vs. Ensembl Sea Lamprey
Match: ago2 (argonaute RISC catalytic component 2 [Source:ZFIN;Acc:ZDB-GENE-110606-6]) HSP 1 Score: 135.191 bits (339), Expect = 1.969e-35 Identity = 76/227 (33.48%), Postives = 123/227 (54.19%), Query Frame = 1
Query: 2305 MVVGLDVWHDTKGRSR--SVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNK-GQINN-PNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNL 2973
+ +G +V H G + S+ AVVGS +A +R+ + +Q +EI+ L + + LL +++ F P I YRDGVS+GQ V E+ DY+P + Y VQKR +TRL+ +D + G+ N P T VD +T + +DF+L S + QGT P+HY VL D+++++ ++Q++ Y+ CH Y +V +PAP YA +A+ +L
Sbjct: 11 IFLGAEVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQHHRQEIIQDLGTMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLFHELLAIRDACIKLEKDYQPGITYVAVQKRHHTRLFCADRNERIGKSGNIPAGTTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSADELQVLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHL 237
BLAST of SMEDWI-3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009610.1 (pep scaffold:Pmarinus_7.0:GL477777:44890:66775:1 gene:ENSPMAG00000008651.1 transcript:ENSPMAT00000009610.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 102.834 bits (255), Expect = 1.221e-22 Identity = 91/331 (27.49%), Postives = 156/331 (47.13%), Query Frame = 1
Query: 1744 WGITIEKDTVNINACKMNPEMI-YFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWIL--ISPKSLLTKA--NGLLQALMSKSPRVGV-MFGKPKIVE--MNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVV-LAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIP-------IKKFMVVGLDVWHDTKGRSR--SVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSD 2682
+GI ++ D ++ +NP M+ Y G +V+ Q + + + GI I+ W + +P+ + L S G+ + G+P + + E + LK N G+QL+V IL + Y +K+ + +Q V + LK P+ S N+ L+IN KLGG + + +P + + +G DV H G + S+ AVVGS +A +R+ + +Q+ +EI++ L S + LL +++ F P I YRDGVS+GQ V E+ + +Y+P + + VVQKR +TRL+ +D
Sbjct: 398 FGIMVKDDMADVQGRVLNPPMLQYGGRTRAVATPNQGVWD--MRGKQFFTGIEIKVWAIACFAPQRQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGAENVEPMFRHLK-NAYMGLQLIVVILPG-KTPVYAEVKRVGDTLLGMATQCVQVKNVLKTSPQTLS---NLCLKINVKLGG-VNNILVPHIRPAVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQQHRQEIIHDLSSMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLYHELLAIREACIRLEKEYRPGITFIVVQKRHHTRLFCAD 720
BLAST of SMEDWI-3 vs. Ensembl Nematostella
Match: EDO34027 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQI3]) HSP 1 Score: 637.876 bits (1644), Expect = 0.000e+0 Identity = 325/768 (42.32%), Postives = 488/768 (63.54%), Query Frame = 1
Query: 730 LTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPM-VYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD-KGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLV---YLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
+ LS NY+++ + ++QY VS++P ID+R R ++Q G +AFD L++P+ L T ++ + +D + V V + FT V + VYN+LF+R+ L M ++GR ++ P+ + + + +WPGY+TS+Q+++GGL+L CDV H+++R DS D M+ ++ V ++ + + + +L+G +VLT +NN+ YR+ DIDW K S F GE++++ +Y+K +Y ++IRDL+QPMIV++ PK +++ GG++ LIPELC LTGL D M KDFR+M+++ H RV P +R EA+ +FV +I S EA + L W + ++ T+ + PE I +GN SAG+QA++ + KVI + + NW++ + KAN +Q + +P +G+ +PK+ ++ +DRTE YL+ ++ N+ P VQ+VV I R+DRY A+KK C V+ PVPSQV++++T+ KL SV IALQIN KLGGELWA+ IP + MV G+DV+HD RSVG +V S N TRW+S Q +E++ L+ ++ ++ + + NN LPE I +YRDGV DGQL V E++QF + F SF YKP + VVQKRINTR++ ++P+ + NP PG+++DHTVTR + DFFLVSQ VRQGTVTPTHYVV+ D S + P +Q ++YK H+YYNWPGTVRVPAPC YAHKLAY VGQ++ +PS LCDRLF+L
Sbjct: 1 IPLSCNYIRIHCPEQAVFQYCVSYSPEIDNRSERFHLLQ----QHPGFRGPQKAFDSTMLFLPRRLPQPVTQLQSTRRSDGSPVTVTITFTRVVPPDDAIFQVYNILFRRVMKILDMQQVGRYFYNPKTPAHIPQHNLVLWPGYITSLQHYEGGLMLLCDVHHRLLRTDSCLDYMLNMHSKVGDRFRDEVS--KELIGSVVLTTYNNKTYRVDDIDWNKRPTSTFTFHNGEQMTFVEYYKKSYEIEIRDLDQPMIVNR----------PKKTEQKRGGVIEVILLIPELCKLTGLNDAMRKDFRVMKDIAAHTRVNPMQRQEAMKKFVMSINSTPEALEQLTAWNLRLDTSTLMTEGRQFPPEKIIYGNTTG-SAGDQADWGRDATREKVISAVNLNNWMVFFTQRDSGKANDFVQTMNKCAPAMGIQVNQPKMNQLRDDRTETYLRSIRENLHPQVQVVVVIFPTSRDDRYAAVKKLCCVESPVPSQVIISKTISNPTKLRSVVQKIALQINVKLGGELWALDIPSRSLMVCGIDVYHDKARGGRSVGGLVCSMNRSLTRWYSDVCFQSPGQELIDGLKMLLIKGIRKWHDVNNALPERIIVYRDGVGDGQLKVVAGYEVQQFEECFASFGESYKPKLAVLVVQKRINTRIFSAEPR----LGNPGPGSVLDHTVTRKDWNDFFLVSQHVRQGTVTPTHYVVVYDTSNWKPDIMQKLSYKLTHLYYNWPGTVRVPAPCQYAHKLAYQVGQSIHGQPSQELCDRLFFL 747
BLAST of SMEDWI-3 vs. Ensembl Nematostella
Match: EDO49931 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFC2]) HSP 1 Score: 570.852 bits (1470), Expect = 0.000e+0 Identity = 303/792 (38.26%), Postives = 462/792 (58.33%), Query Frame = 1
Query: 679 PTQIKEPCNTRNDSSPSLTLSANYVKVRT-TQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXX-----XXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNV-APGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTR---WFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQIN--NPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
PT ++ N + + +NY ++ T Q IYQY+VSF+P +D + R ++ G LG + RAFDGM L++P+ L ++ T I + +T + + + + C ++N++ KRI + M ++GR+Y+Y + I + + +E+WPGY TSI ++ +LL DVSHKV+R +V D + E+ + +G A +L+G+IVLT +NN+ YR+ DIDW K S F +IS+ DY+K Y I D EQP++ SK R G + V L+PELC LTGL+DDM DF +M++L H R+ PK R + L + + I + +A L WG+ ++ ++ + + E I+ N+ S + A++ + + + + +ENW+++ A Q L +G+ KP++V +N+DRT+ YLK L + A Q+VVTI+ + R+DRY++IKK C ++ VPSQVV+++TL + L SV I +Q+N KLGGE WAV IP+K M+VG+D +HD+ + RSVG V S N CTR ++S+ Q +E++ L+ CM + L+ + + N LP+ I ++RDGV DGQL V + E+ Q F+ F Y P + VV+KRI TRL++ + ++ N NP PGT+VD VTR+ +DFF+VSQSVRQGTV+PTHY V+ D + P Q ++YK H+YYNWPGTVRVPAPC YAHKLA++VGQ+L EPS L D+L++L
Sbjct: 95 PTTRRDGFNKEGSVGTKVGIVSNYFRLETRPQAAIYQYNVSFSPEVDFKKARFALI-GEQRELLGNI---RAFDGMVLFLPKKLPDQVTKIMAVRTRRETPDEPVEITITLTNELPADSPTCLHLFNIILKRILKYIDMKQVGRNYYYIKNAIAIPKHGLELWPGYTTSILKYEHSILLGADVSHKVLRKQTVLDFLYELYNKMQGRGNFHDEAAKKLVGEIVLTRYNNKTYRVDDIDWNKRPNSTFTTPKGEISFNDYYKKNYDFTITDQEQPLLFSKPKKRKGADDP-----------VLLLPELCFLTGLSDDMRADFSVMKDLAVHTRLDPKARSKDLNKLISEINNNPDASGELSGWGLRFGRNLLDFDGRILPGEKIFQKNKGSNYDPKSADWSREIRGAPLHSTVNLENWVIVHTNRDSGVATDFQQTLARVCGPMGINTTKPRMVPLNDDRTDSYLKALSNEIQADFPQIVVTIVPSNRKDRYDSIKKLCCLEKGVPSQVVVSRTLSKKQMLMSVCTKIGIQLNCKLGGEAWAVDIPLKNLMIVGIDTYHDSSQKGRSVGGFVASLNKPCTRQREYYSRCTFQHSGQELVDGLKVCMTAALRQWQQINGVLPDKIIVFRDGVGDGQLRAVIEHEVPQILSTFKQFQDGYDPKFGFTVVKKRIQTRLFMREARDPDMRNLMNPPPGTVVDKEVTRSEWFDFFIVSQSVRQGTVSPTHYNVIHDTTGLRPDHWQQLSYKLTHLYYNWPGTVRVPAPCQYAHKLAFLVGQSLHKEPSLKLADKLYFL 871
BLAST of SMEDWI-3 vs. Ensembl Nematostella
Match: EDO46431 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQ46]) HSP 1 Score: 166.007 bits (419), Expect = 4.215e-42 Identity = 164/642 (25.55%), Postives = 284/642 (44.24%), Query Frame = 1
Query: 1144 IWPGYVTSIQNFDGGLLLQCDVSHKVI-RNDSVYDIMMEI----------NKTVNNKGQMQ-TAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD---KGGE--KISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIK--DFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQA--LAHNKVIGGIRIENW-ILISPKSLLTKANGLLQ---ALMSKSPRVGVMFGKPKIV---EMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTL-KEGPKLNSVAVNIALQINAKLGG--ELWAVKI--PI--KKFMVVGLDVWHDTKGRSR--SVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNK-GQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAY 2955
+W G+ SI+ +LL DVS + SV + M E+ + +N+ +++ T I L +I R YR+ ++ +F + G+ +I+ YF++ + ++R P + R YL E+C + + K D + + + R P + E + V E+A + + I+I K+ V + + P + +G + + + F + ++ GI I W I K + + L + LM S G+ P + N D E + LK P +Q+++ +L + Y +K+ V +Q + + + K P+ S N+ L+INAKLGG + A +I P+ + + +G DV H G + SV AVVGS +A +R+++ +Q +EI+ L + + LL ++ P+ I YRDGVS+GQ V E++ + Y+P + + VVQKR +TRL+ ++K G+ N PGT VD +T +DF+L S + QGT P+HY VL D++ ++ ++Q + Y+ CH Y V +PAP YAH +A+
Sbjct: 189 VWFGFHQSIRPSQWKMLLNIDVSATAFYKCQSVVEFMCEVLRIRKDDLEKHAPLNDADRLRFTREIKGLKVEITHCGPMKRKYRVINVTKQPAQALQFPLTLESGQTAQITVARYFQDKHKQRLRYPHLPCLQVGQEQRH----------------TYLPLEVCNIVPGQRCVKKLTDTQTSKMIRATARSAPDRERE-IRGLVKKANFDEDA--YVKDFSISIGKNMVELQGRVLPPPKLVYGGKVRIEKIIHS-FSGVWDMRGRQLFHGIEIRTWAIACFVKQQMCTEDSLRRFSNQLMKISVEQGMPISCPPVFFRYARNPDEVERMFRRLK-EAHPDLQMILVVLPG-KTPVYAEVKRVGDTMLGVITQCIQGKNVTKPSPQTLS---NLCLKINAKLGGVNNILAPEIRPPVFREPVIFLGADVTHPAAGDDKRPSVAAVVGSMDAHPSRYYASVRVQTHRQEIIAELAAMVRELLVQFYRSTRHKPQRIVFYRDGVSEGQFRQVLVHELKAIREACIKLEVGYQPGISFIVVQKRHHTRLFCQRDQDKCGKSGNVPPGTTVDRGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNNFSADELQALTYQLCHTYVRCTRAVSIPAPAYYAHLVAF 805
BLAST of SMEDWI-3 vs. Ensembl Nematostella
Match: EDO28124 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T7C9]) HSP 1 Score: 92.4337 bits (228), Expect = 1.665e-21 Identity = 59/180 (32.78%), Postives = 81/180 (45.00%), Query Frame = 1
Query: 2296 KKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEI-------------------------------------------------MYVL-QSCML-SLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSD 2682
+ MV G+DV+HD RSVG +V S N TRW+S Q +E+ MYV+ C+L ++ + + NN LPE I +YRDGV DGQL V E++QF + F SF YKP + VVQKRINTR++ ++
Sbjct: 1 RSLMVCGIDVYHDKARGGRSVGGLVCSMNRSLTRWYSDVCFQSPGQELIDGLKMLLIKGIRKWHDAIRKWHDVCYLTMFVSLSRPSGSGMMPSGSGMMYVIFNVCVLIKAIRKWHDVNNALPERIIVYRDGVGDGQLKVVAGYEVQQFEECFASFGESYKPKLAVLVVQKRINTRIFSAE 180
BLAST of SMEDWI-3 vs. Ensembl Nematostella
Match: EDO28197 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T755]) HSP 1 Score: 77.7962 bits (190), Expect = 1.100e-17 Identity = 37/70 (52.86%), Postives = 52/70 (74.29%), Query Frame = 1
Query: 2086 QLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPI 2295
Q+VVTI+ + R+DRY++IKK C ++ VPSQVV+++TL + L SV I +Q+N KLGGE WAV IP+
Sbjct: 1 QIVVTIVPSNRKDRYDSIKKLCCLEKGVPSQVVVSRTLSKKQMLMSVCTKIGIQLNCKLGGEAWAVDIPV 70
BLAST of SMEDWI-3 vs. Ensembl Medaka
Match: piwil1 (piwi like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:100301587]) HSP 1 Score: 560.836 bits (1444), Expect = 0.000e+0 Identity = 310/788 (39.34%), Postives = 466/788 (59.14%), Query Frame = 1
Query: 670 REYPTQIKEP-CNTRNDSSPSLTLSANYVKVRT-TQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLY--VSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
R+ +KE C T S + L+AN+ ++ + Q +YQYHV F PP++SR +R ++ +E V+ +AR FDG L +P+ L KET++ + + V+ + S C YN+LFKR+ L M +IGR+Y+ P+ + + + IWPGY T+I ++ ++L DVSHKV+R+++V D M+ + + N+ + A +L+G IVLT +NN+ YRI DI W + F +G IS+ +Y+K YGL I D+ Q ++VS R+G GP + LIPELC LTGLTD M DF +MR+L E+ R++P KR L F+++I ++ L WGI + +N++A + PE I+ G + ++ + + +I + NWI+ S + +A LLQ L S +G+ + ++E D E L+ LK+NV P Q+VV +L + R+D+Y+++KK+ VDCP PSQ VL++TL L +VA IALQ++ K+GGELW+V+IP+K+ M+VG+D +HD RS+GA+V S N +RW+SK LQ + +E+M L+ LK Y + NN LP I +YRDGV DGQL V EI Q + +S DY P + VV+KRI++R + ++D +++NP PGT++D VTR YDFF+VSQ+VR G+V+PTHY V+ D S P +Q + YK CH+YYNW G +RVPAPC YAHKLA++VGQ++ EP+ L + LFYL
Sbjct: 88 RQLMEHVKESKCGT---SGSPIQLTANFYRILSRPQWVLYQYHVDFKPPMESRRLRTALLYRHAE----VIGDARTFDGALLILPRRLHCKETVLYSETKSGEKVLITVTLTNELPPTSPVCIQFYNILFKRVLRLLEMQQIGRNYYNPKNPLNIPEHNLTIWPGYSTTILQYESSIMLCTDVSHKVLRSETVLDFMVNLRQKCGNQ-RFPDACAKELIGLIVLTKYNNKTYRIDDIAWDHTPKNTFTRGDADISFMNYYKTQYGLDITDVNQVLLVSHVKKRTGPGGGPPLGPAM------LIPELCYLTGLTDKMRSDFTIMRDLSENTRLSPDKRISRLSGFIEDICGNAGVQEELKKWGIGFDNHLLNLSARVLAPERIFQGPRSYDYNPMSGDWSREMRGIPIISSPPLNNWIMFSTRRNSNEAQSLLQTLGKVSGPLGLRLQRAVMIEYE-DHQESLLRALKQNVGPTTQMVVVVLPSNRKDKYDSVKKYLCVDCPTPSQCVLSRTLSRPQALMTVATKIALQMSCKMGGELWSVEIPLKQLMIVGIDCYHDISAGKRSIGAMVASLNQSMSRWYSKCVLQHKGQELMDGLKMAFTGALKDYLKFNNCLPSRIIVYRDGVGDGQLHSVVNFEISQILESIKSLGQDYNPKVSVVVVKKRISSRFFAMIND-----KVSNPPPGTVIDTEVTRPEWYDFFIVSQAVRCGSVSPTHYNVVYDTSGLKPDHMQRLTYKLCHMYYNWQGLIRVPAPCQYAHKLAFLVGQSIHREPNIKLDNYLFYL 855
BLAST of SMEDWI-3 vs. Ensembl Medaka
Match: piwil1 (piwi like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:100301587]) HSP 1 Score: 553.518 bits (1425), Expect = 0.000e+0 Identity = 310/788 (39.34%), Postives = 467/788 (59.26%), Query Frame = 1
Query: 670 REYPTQIKEP-CNTRNDSSPSLTLSANYVKVRT-TQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLY--VSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
R+ +KE C T S + L+AN+ ++ + Q +YQYHV F PP++SR +R ++ +E V+ +AR FDG L +P+ L KET++ + + V+ + S C YN+LFKR+ L M +IGR+Y+ P+ + + + IWPGY T+I ++ ++L DVSHKV+R+++V D M+ + + N+ + A +L+G IVLT +NN+ YRI DI W + F +G IS+ +Y+K YGL I D+ Q ++VS R+G GP + LIPELC LTGLTD M DF +MR+L E+ R++P KR L F+++I ++ L WGI + +N++A + PE I+ G + ++ + + +I + NWI+ S + +A LLQ L S +G+ + ++E D E L+ LK+NV P Q+VV +L + R+D+Y+++KK+ VDCP PSQ VL++TL L +VA IALQ++ K+GGELW+V+IP+K+ M+VG+D +HD RS+GA+V S N +RW+SK LQ + +E+M L+ + LK Y + NN LP I +YRDGV DGQL V EI Q + +S DY P + VV+KRI++R + ++D +++NP PGT++D VTR YDFF+VSQ+VR G+V+PTHY V+ D S P +Q + YK CH+YYNW G +RVPAPC YAHKLA++VGQ++ EP+ L + LFYL
Sbjct: 58 RQLMEHVKESKCGT---SGSPIQLTANFYRILSRPQWVLYQYHVDFKPPMESRRLRTALLYRHAE----VIGDARTFDGALLILPRRLHCKETVLYSETKSGEKVLITVTLTNELPPTSPVCIQFYNILFKRL---LEMQQIGRNYYNPKNPLNIPEHNLTIWPGYSTTILQYESSIMLCTDVSHKVLRSETVLDFMVNLRQKCGNQ-RFPDACAKELIGLIVLTKYNNKTYRIDDIAWDHTPKNTFTRGDADISFMNYYKTQYGLDITDVNQVLLVSHVKKRTGPGGGPPLGPAM------LIPELCYLTGLTDKMRSDFTIMRDLSENTRLSPDKRISRLSGFIEDICGNAGVQEELKKWGIGFDNHLLNLSARVLAPERIFQGPRSYDYNPMSGDWSREMRGIPIISSPPLNNWIMFSTRRNSNEAQSLLQTLGKVSGPLGLRLQRAVMIEYE-DHQESLLRALKQNVGPTTQMVVVVLPSNRKDKYDSVKKYLCVDCPTPSQCVLSRTLSRPQALMTVATKIALQMSCKMGGELWSVEIPLKQLMIVGIDCYHDISAGKRSIGAMVASLNQSMSRWYSKCVLQHKGQELMDGLKMA-FTALKDYLKFNNCLPSRIIVYRDGVGDGQLHSVVNFEISQILESIKSLGQDYNPKVSVVVVKKRISSRFFAMIND-----KVSNPPPGTVIDTEVTRPEWYDFFIVSQAVRCGSVSPTHYNVVYDTSGLKPDHMQRLTYKLCHMYYNWQGLIRVPAPCQYAHKLAFLVGQSIHREPNIKLDNYLFYL 821
BLAST of SMEDWI-3 vs. Ensembl Medaka
Match: piwil2 (piwi like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:100301588]) HSP 1 Score: 554.673 bits (1428), Expect = 7.435e-180 Identity = 292/779 (37.48%), Postives = 449/779 (57.64%), Query Frame = 1
Query: 694 EPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFD-KGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVA--PGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
+P N S+T+ +N++ + +YQYHV+F P ++S MR G+ E AFDG LY+P L + + V + D+ + + S+ C YNV+F+R+ + + +GR+++ PE ++L ++R+++WPGY T+I+ DGGL L DVSHKV+RNDSV D+M + + K + A +L+G I +T +NNR YRI DI+W K+ F G + ++ +Y+ YG+ +++ QP++V + RS N G K V G + L+PEL LTG+ + M D+R M++L H V+ + A+ + + NI S E+ K L WG+ + D + I + E I +S + G + + + + I I + W++ P+ +A L+ + +G+ +P VE+ +DRTE Y+K + + P +QLVV ++ R+D Y+AIKK C + CPVPSQV+ +T+ + KL +A I LQ+N+KLGGELW V IP+K MVVG+DV HDT + +SV V S N+ T W+S+ Q +E++ ++C LS L+ Y+E N+ LP+ I +YRDGVSDGQL V+ EI Q K FE+F +Y+P +V+ VVQKRINT LY S + GT++DHT+T + DF+L + +RQG PTHYV L + + TP +Q + +K CH+Y+NWPGT+RVPAPC YAHKLA++ GQ L +EP+ L D+LF+L
Sbjct: 273 KPLNKAGVKGASITIGSNHIPISCKNQAVYQYHVTFNPNVESMSMRF----GMMRDHRSTTGEVIAFDGSILYLPVQLCDVVNLKSVRRTDDEEIEITIKMTKVLPPNSDLCIPFYNVVFRRVMKVIGLRLVGRNHYDPENAVILGKQRLQVWPGYSTAIKRMDGGLYLAVDVSHKVLRNDSVLDLMNTLYQ--QTKENFKDACTKELIGSIAITRYNNRTYRIDDIEWDKSPKDSFTLMDGTETTFLEYYSKNYGITVQETNQPLLVHRPKERS--NPGGK----VISGHILLVPELSFLTGIPEKMKNDYRAMKDLKIHINVSSDQHTHAIKQLLKNISSKEDCTKALEGWGLELGSDVMMITGRMLPFETICL-KSSSFATGANVSWSREVVNGASISSIPLNIWVVFYPRRSAEQAVELISTFHKVAGPIGLRLQRPITVELRDDRTETYVKSIHSQLTSEPHLQLVVCVIEGNRDDLYSAIKKLCCLKCPVPSQVINVRTISQQQKLRGIAQKILLQLNSKLGGELWTVGIPLKSLMVVGVDVHHDTSKKHQSVMGFVASVNSSLTHWYSRVTFQTPTEELISGFRACFLSTLQKYYELNHDLPQKIVVYRDGVSDGQLKMVELYEIPQLIKCFETF-PNYEPKLVFIVVQKRINTILYSSSANGFSSVPA---GTVLDHTLTNKDWVDFYLTAHDIRQGVGLPTHYVTLYNTANLTPDHLQRLTFKMCHLYWNWPGTIRVPAPCKYAHKLAFLSGQFLHSEPAIQLSDKLFFL 1034
BLAST of SMEDWI-3 vs. Ensembl Medaka
Match: piwil1 (piwi like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:100301587]) HSP 1 Score: 499.204 bits (1284), Expect = 4.787e-158 Identity = 287/749 (38.32%), Postives = 436/749 (58.21%), Query Frame = 1
Query: 670 REYPTQIKEP-CNTRNDSSPSLTLSANYVKVRTT-QPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLY--VSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNW 2901
R+ +KE C T S + L+AN+ ++ + Q +YQYHV F PP++SR +R ++ +E V+ +AR FDG L +P+ L KET++ + + V+ + S C YN+LFKR+ L M +IGR+Y+ P+ + + + IWPGY T+I ++ ++L DVSHKV+R+++V D M+ + + N+ + A +L+G IVLT +NN+ YRI DI W + F +G IS+ +Y+K YGL I D+ Q ++VS R+G GP + LIPELC LTGLTD M DF +MR+L E+ R++P KR L F+++I ++ L WGI + +N++A + PE I+ G + ++ + + +I + NWI+ S + +A LLQ L S +G+ + ++E D E L+ LK+NV P Q+VV +L + R+D+Y+++KK+ VDCP PSQ VL++TL L +VA IALQ++ K+GGELW+V+IP+K+ M+VG+D +HD RS+GA+V S N +RW+SK LQ + +E+M L+ LK Y + NN LP I +YRDGV DGQL V EI Q + +S DY P + VV+KRI++R + ++D +++NP PGT++D VTR YDFF+VSQ+VR G+V+PTHY V+ D S P +Q + YK CH+YYNW
Sbjct: 88 RQLMEHVKESKCGT---SGSPIQLTANFYRILSRPQWVLYQYHVDFKPPMESRRLRTALLYRHAE----VIGDARTFDGALLILPRRLHCKETVLYSETKSGEKVLITVTLTNELPPTSPVCIQFYNILFKRVLRLLEMQQIGRNYYNPKNPLNIPEHNLTIWPGYSTTILQYESSIMLCTDVSHKVLRSETVLDFMVNLRQKCGNQ-RFPDACAKELIGLIVLTKYNNKTYRIDDIAWDHTPKNTFTRGDADISFMNYYKTQYGLDITDVNQVLLVSHVKKRTGPGGGPPLGPAM------LIPELCYLTGLTDKMRSDFTIMRDLSENTRLSPDKRISRLSGFIEDICGNAGVQEELKKWGIGFDNHLLNLSARVLAPERIFQGPRSYDYNPMSGDWSREMRGIPIISSPPLNNWIMFSTRRNSNEAQSLLQTLGKVSGPLGLRLQRAVMIEYE-DHQESLLRALKQNVGPTTQMVVVVLPSNRKDKYDSVKKYLCVDCPTPSQCVLSRTLSRPQALMTVATKIALQMSCKMGGELWSVEIPLKQLMIVGIDCYHDISAGKRSIGAMVASLNQSMSRWYSKCVLQHKGQELMDGLKMAFTGALKDYLKFNNCLPSRIIVYRDGVGDGQLHSVVNFEISQILESIKSLGQDYNPKVSVVVVKKRISSRFFAMIND-----KVSNPPPGTVIDTEVTRPEWYDFFIVSQAVRCGSVSPTHYNVVYDTSGLKPDHMQRLTYKLCHMYYNW 816
BLAST of SMEDWI-3 vs. Ensembl Medaka
Match: piwil1 (piwi like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:100301587]) HSP 1 Score: 499.204 bits (1284), Expect = 4.787e-158 Identity = 287/749 (38.32%), Postives = 436/749 (58.21%), Query Frame = 1
Query: 670 REYPTQIKEP-CNTRNDSSPSLTLSANYVKVRTT-QPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSE-CPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLY--VSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNW 2901
R+ +KE C T S + L+AN+ ++ + Q +YQYHV F PP++SR +R ++ +E V+ +AR FDG L +P+ L KET++ + + V+ + S C YN+LFKR+ L M +IGR+Y+ P+ + + + IWPGY T+I ++ ++L DVSHKV+R+++V D M+ + + N+ + A +L+G IVLT +NN+ YRI DI W + F +G IS+ +Y+K YGL I D+ Q ++VS R+G GP + LIPELC LTGLTD M DF +MR+L E+ R++P KR L F+++I ++ L WGI + +N++A + PE I+ G + ++ + + +I + NWI+ S + +A LLQ L S +G+ + ++E D E L+ LK+NV P Q+VV +L + R+D+Y+++KK+ VDCP PSQ VL++TL L +VA IALQ++ K+GGELW+V+IP+K+ M+VG+D +HD RS+GA+V S N +RW+SK LQ + +E+M L+ LK Y + NN LP I +YRDGV DGQL V EI Q + +S DY P + VV+KRI++R + ++D +++NP PGT++D VTR YDFF+VSQ+VR G+V+PTHY V+ D S P +Q + YK CH+YYNW
Sbjct: 88 RQLMEHVKESKCGT---SGSPIQLTANFYRILSRPQWVLYQYHVDFKPPMESRRLRTALLYRHAE----VIGDARTFDGALLILPRRLHCKETVLYSETKSGEKVLITVTLTNELPPTSPVCIQFYNILFKRVLRLLEMQQIGRNYYNPKNPLNIPEHNLTIWPGYSTTILQYESSIMLCTDVSHKVLRSETVLDFMVNLRQKCGNQ-RFPDACAKELIGLIVLTKYNNKTYRIDDIAWDHTPKNTFTRGDADISFMNYYKTQYGLDITDVNQVLLVSHVKKRTGPGGGPPLGPAM------LIPELCYLTGLTDKMRSDFTIMRDLSENTRLSPDKRISRLSGFIEDICGNAGVQEELKKWGIGFDNHLLNLSARVLAPERIFQGPRSYDYNPMSGDWSREMRGIPIISSPPLNNWIMFSTRRNSNEAQSLLQTLGKVSGPLGLRLQRAVMIEYE-DHQESLLRALKQNVGPTTQMVVVVLPSNRKDKYDSVKKYLCVDCPTPSQCVLSRTLSRPQALMTVATKIALQMSCKMGGELWSVEIPLKQLMIVGIDCYHDISAGKRSIGAMVASLNQSMSRWYSKCVLQHKGQELMDGLKMAFTGALKDYLKFNNCLPSRIIVYRDGVGDGQLHSVVNFEISQILESIKSLGQDYNPKVSVVVVKKRISSRFFAMIND-----KVSNPPPGTVIDTEVTRPEWYDFFIVSQAVRCGSVSPTHYNVVYDTSGLKPDHMQRLTYKLCHMYYNW 816
BLAST of SMEDWI-3 vs. Planmine SMEST
Match: SMESG000081970.1 (SMESG000081970.1) HSP 1 Score: 1885.92 bits (4884), Expect = 0.000e+0 Identity = 959/960 (99.90%), Postives = 959/960 (99.90%), Query Frame = 1
Query: 139 LLMQKFLNKDVLVPSVESLEDKALNKLGIPLPGSTVEKNTEXXXXXXXXXXXXXXXXXXXNIKLQDSDIENRNITIVTRPLSCIGRGRGLSNPSSLTTSSGKSDKITENEEPGQIKKFVGRGRGLLNSQKECSNSTPSEVSNELKQMKISNDDKMTVSSEAKSQFENIEKPISKFRRREYPTQIKEPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIXXXXXXXXXXXXXXXXXXXXXXXSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 3018
LLMQKFLNKDVLVPSVESLEDKALNKLGIP PGSTVEKNTESSSISSGDSRSNPSGDSRNNIKLQDSDIENRNITIVTRPLSCIGRGRGLSNPSSLTTSSGKSDKITENEEPGQIKKFVGRGRGLLNSQKECSNSTPSEVSNELKQMKISNDDKMTVSSEAKSQFENIEKPISKFRRREYPTQIKEPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIKVNKPTDKTVVDVKVVFTNNVNFSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL
Sbjct: 15 LLMQKFLNKDVLVPSVESLEDKALNKLGIPPPGSTVEKNTESSSISSGDSRSNPSGDSRNNIKLQDSDIENRNITIVTRPLSCIGRGRGLSNPSSLTTSSGKSDKITENEEPGQIKKFVGRGRGLLNSQKECSNSTPSEVSNELKQMKISNDDKMTVSSEAKSQFENIEKPISKFRRREYPTQIKEPCNTRNDSSPSLTLSANYVKVRTTQPHIYQYHVSFAPPIDSRLMRIKIVQGLSESDLGVVKEARAFDGMNLYIPQLLKNKETIIKVNKPTDKTVVDVKVVFTNNVNFSECPMVYNVLFKRIENSLRMVKIGRDYFYPEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCDRLFYL 974
BLAST of SMEDWI-3 vs. Planmine SMEST
Match: SMESG000036375.1 (SMESG000036375.1) HSP 1 Score: 240.736 bits (613), Expect = 1.275e-66 Identity = 190/699 (27.18%), Postives = 329/699 (47.07%), Query Frame = 1
Query: 1039 NVLFKRIENSLRMVKIGRDYFYP---------EKKIVLDRRRMEIWPGYVTSI-----QNFDGGLLLQCDVSHKVIRNDSVYDIMMEINKTVNNKGQMQTAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNS-----RSGKNRGPKGSKEVDGGLVYLIP-ELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTKANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDR-YNAIKKFCYVDCPVPSQVVLAQ--TLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFES-FSADYKPNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYT-----------PHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNLLCD----RLFYL 3018
N++F I+ + KIGR +F E +I GY T+I +N LL + ++V+ ++V + + I+QL+G+ ++T +NN+ YRI+DI + E G +KISY +YFK Y + +++ +QP ++S+ + P+G E D L IP ELC L G +D + L + L + P++R + +F ++K + WG+ IE+ + I ++ P I F NE ++ ++K V G +L+ ++ + N ++ + + R+ + + I ++ E+ L + R V V L + +D+ Y +K F + + +Q V + + ++ + +VA +Q+ +KLG + W + + + M+VGLD +H G+ RSV A V S +A +++ S + + E L L+ L + + N +P + +YRDGV D QL + +K E + K+ E + P ++Y VV+KRI+ + + NPNPGT+VD + + N Y+F+LVSQ +GT +PT+Y VL D +K+T P +Q + Y H+Y+NW GT+RVP P YAH+LA +VG+ + + D RLFYL
Sbjct: 144 NIVFNNIQIFMGQEKIGRQFFLGSNCESGGIREGPSFFKMDNFKILGGYSTTITRGTNENGTASTLLYLERINRVLNENNVVSVY-------------RRDQIDQLIGRDIITKYNNKTYRISDIK-EMDVNDEVQLGDKKISYLNYFKQRYNINLKNDKQPFVISRVKRSMVRPKEKTENEPEGPTETDQSLS--IPGELCYLCGFSDSERSNINLQKNLGTVLKREPRERLNDIRDFC-KWTGAGKSKDYMKSWGMCIEEQPITIRGRELAPVDI-FSNEVKINTKIPDDWKFGEIKFDVPKGTAHRFGVLVVDRNP-SHFNNFIEDVKREIGRLRINYTMDSISSCRSNEVEDALTDFIR-----VSKVHMALVFIPDDKVYAKVKNFT-MSTGLLTQCVTQRNGSNRDDRRRKTVADKSVMQMFSKLGYDPWGINLKMAPTMIVGLDTFHSKTGK-RSVQASVFSISAKFSQYISFVNSSKGKNEFHENLGKNFLTALTTFQNKFNTMPLRLIIYRDGVGDSQLAFTKKFETDAVMKMIEKIYENQTLPQIIYVVVKKRISVKFFKDGA-------NPNPGTVVDEKIVKPNFYEFYLVSQKTTKGTASPTNYNVLMD-TKFTNKKTNEVSVMSPSVLQQITYSLTHLYFNWMGTIRVPVPTHYAHRLAELVGKIHRGVTPPAINDRIRERLFYL 808
BLAST of SMEDWI-3 vs. Planmine SMEST
Match: SMESG000069984.1 (SMESG000069984.1) HSP 1 Score: 223.402 bits (568), Expect = 1.291e-60 Identity = 174/629 (27.66%), Postives = 310/629 (49.28%), Query Frame = 1
Query: 1258 INKTVNNKGQMQT---AAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEFDKGGEKISYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDG--------------GLVYLIP-ELCMLTGLTDDMIKDFRLMRELHEHCRVTPKKRHEALLEFVDNIYSCEEAKKLLGYWGITIEKDTVNINACKMNP-EMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWILISPKSLLTK--ANGLLQALMSKSPRVGVMFGKPKIVEMNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQ--TLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMVVGLDVWHDTKGRSRSVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYK------PNMVYNVVQKRINTRLYVSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYT---------PHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEP----SNLLCDRLFYL 3018
IN+ +N+ M+ +I+QL+G+ ++T +NN+ YRI++I N +F+ GG +SY +YFK Y +++ +QP ++++ + R K + V+ + IP ELC L G +D + L + L + P++R + + + + I + + A + W + I+ + I ++ P ++I G++ + G+ +F + K I+ +++ K N + Q L ++ R+ GK N+ E L + R+ + ++ + V +DR A K + + +Q V + T + + V+ +QI AK G + W V+I ++ M+VG+D +H+ K +S+ A V S N+ T++ S + + +E L L+ + + + LP+ I ++RDGV D QL + + E++ + E+ YK P ++Y +V+KR+ T+L+ N+G NNPNPGT+VD + + N Y+FFLVSQ +GT TPT+Y VL D T P+++Q + Y H+Y+NW GT+RVP P YAH+LA +VG+ K + + +RLFYL
Sbjct: 228 INRVLNDNSVMKAYNRRSIDQLIGRDIITKYNNKTYRISEIK-EMNVDEKFEMGGRTLSYAEYFKERYNIRLTQGDQPFVLTRVKKPMRRERKKKDEEGVEKEKEKEREKEAPEEKDMTLNIPGELCFLCGFSDQEKSNMDLQKNLGCVLKREPRERLDDIPAYCNWIKNSDAATGMCNKWQLKIDNKPLEIEGRELPPCDVISGGSKINEKMGDDWKFGRVQFDIKRDRKHEID-VVIVDRNDFQYKNFMNDVEQEL--RNMRIDARVGKVNTCGPND--VERCLNDAARSGSGCAKMALVF---VPDDRVYAKVKSFTMSTGLLTQCVTTRNGTNRNDKRRKVVSSKTVMQIFAKFGYDPWTVEIKLRPTMIVGMDTYHN-KSSKKSIQASVFSINSTFTQYMSFVNSPKGRQEFHETLGKNFNLALEDFKKRYDILPQRILVFRDGVGDNQLQFTKNFEVDAMKPLIENI---YKGNGFPVPQIIYVIVKKRVGTKLF-----NRG--NNPNPGTVVDKEIVKPNFYEFFLVSQRTTKGTATPTNYNVLEDTRLLTKKGTMDPMAPNELQKITYALTHLYFNWMGTIRVPVPVHYAHRLAELVGKVHKGSNPVAINERIRNRLFYL 836
BLAST of SMEDWI-3 vs. Planmine SMEST
Match: SMESG000047591.1 (SMESG000047591.1) HSP 1 Score: 168.703 bits (426), Expect = 1.733e-42 Identity = 166/668 (24.85%), Postives = 296/668 (44.31%), Query Frame = 1
Query: 1066 SLRMVKIGRDYFY--PEKKIVLDRRRMEIWPGYVTSIQNFDGGLLLQCDVSHKVI-RNDSVYDIMMEI-------NKTVNNKGQMQ-TAAINQLLGQIVLTPHNNRNYRITDIDWAKNCLSEF---DKGGEKI--SYRDYFKNTYGLQIRDLEQPMIVSKSNSRSGKNRGPKGSKEVDGGLVYLIPELCMLTGLTDDMIK--DFRLMRELHEHCRVTPKKRHEALLEFVDNIY--SCEEAKKLLGYWGITIEKDTVNINACKMNPEMIYFGNEASVSAGEQAEFKQALAHNKVIGGIRIENWIL--ISPKSLLTKAN--GLLQALMSKSPRVGV-MFGKPKIVE--MNNDRTEEYLKELKRNVAPGVQLVVTILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTL-KEGPKLNSVAVNIALQINAKLGGELWAVKIPI-------KKFMVVGLDVWHDTKGRSR--SVGAVVGSTNALCTRWFSKSHLQEQDKEIMYVLQSCMLSLLKAYFEENNFLPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNK-GQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAY 2955
S+ +GR +F+ + L R E+W G+ S++ ++L DVS + SV + M E+ + + + +++ T I L +I R YR+ ++ F + G+ I + YF++ YG++++ P + + YL E+C + + K D + + R P + E ++N+ S A L +G+++ +I + I +G A Q + + GI I+ W + +P+ + + + Q L S G+ + G+P + D+ E + LK N G+QL+V +L + Y +K+ + + +Q V A+ + K P+ S N+ L+IN KLGG + ++ +P + + +G D H G S+ AVVGS +A +R+ + +Q +E + L + + LL +++ F P I YRDGVS+GQ V E++ + Y+P + + VVQKR +TRL+ SD K++ G+ N GT VD +T +DF+L S + QGT P+HY VL D+++++ ++Q + Y+ CH Y +V +PAP YAH +A+
Sbjct: 242 SMTYTPVGRSFFHNTGDYDNPLGGGR-EVWFGFHQSVRPSQWKMMLNIDVSATAFYKEQSVINFMFEVLEIDKDQKRPLTDSQRVKFTKEIKGLKVEINHCGPIKRKYRVCNVTRRSAQSQTFPLQSEQGQTIECTVTKYFQDRYGMKLQHPHLPCLQVGQEQKH----------------TYLPLEVCNIVSGQRCIKKLTDMQTSTMIKATARSAPDREKE-----INNLVRRSNFNADPHLQIFGVSVNTRMADIQGRVIPAPKIQYGGRTKAQATPQQGVWD-MRGKQFFSGIEIKVWAIACFAPQRTVREDSLRTFTQQLQKISNDAGMPIVGQPCFCKYATGQDQVEPMFRYLK-NSFTGLQLIVVVLPG-KTPVYAEVKRVGDIIFGLATQCVQAKNVNKPSPQTLS---NLCLKINVKLGG-INSILVPSVRPAVFKEPVIFLGADFTHPPAGDKTKPSIAAVVGSMDAHPSRYAATVSVQPHRQETIQDLAAMVKELLIQFYKSTRFKPTRIIYYRDGVSEGQFLNVLNHELKAIREACIKLEMSYQPGITFVVVQKRHHTRLFCSDKKDQMGRSGNIPAGTTVDQIITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSADEIQTLTYQLCHTYVRCTRSVSIPAPAYYAHLVAF 880
BLAST of SMEDWI-3 vs. Planmine SMEST
Match: SMESG000051000.1 (SMESG000051000.1) HSP 1 Score: 159.458 bits (402), Expect = 1.559e-39 Identity = 109/339 (32.15%), Postives = 178/339 (52.51%), Query Frame = 1
Query: 2023 MNNDRTEEYLKELKRNVAPGVQLVVTILSAVRED-RYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVK----IPIKK-FMVVGLDVWH--DTKGRSRSVGAVVGSTNALCTRWFS--KSHLQEQDK---EIMYVLQSCMLSLLKAYFEENNF-LPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLY-VSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNL 2994
++ND ++ ++++K ++ L+V + VR D Y +K ++ + +Q + + L L+ N+ L++NAKLGG W +K PIK FMV G+DV H + G S+SV AVVGS N T + + K L + K E + ++ + L+K +++ N LP+ + YRDGVS+GQ V EI E F +YKP + Y +VQKR N R ++ + G+ N PG ++D+ +T + +DF+L S QGT P HY V+ D +++ + +Q Y C++ + ++ PAP YAH LA G+ LK + N+
Sbjct: 589 VSNDDIKKSIEKIKEDL----DLLVVV---VRNDVEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVETVVEMEDMVHRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAH-LAAFRGRILKDDEDNI 919 The following BLAST results are available for this feature:
BLAST of SMEDWI-3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of SMEDWI-3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of SMEDWI-3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of SMEDWI-3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of SMEDWI-3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of SMEDWI-3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of SMEDWI-3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of SMEDWI-3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMEDWI-3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of SMEDWI-3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 4
BLAST of SMEDWI-3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMEDWI-3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of SMEDWI-3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of SMEDWI-3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30001362 ID=SMED30001362|Name=SMEDWI-3|organism=Schmidtea mediterranea sexual|type=transcript|length=3093bpback to top protein sequence of SMED30001362-orf-1 >SMED30001362-orf-1 ID=SMED30001362-orf-1|Name=SMED30001362-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=975bp MSGSSGIGRGRSRGLLMQKFLNKDVLVPSVESLEDKALNKLGIPLPGSTVback to top Aliases
The feature 'SMEDWI-3' has the following synonyms
Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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