SMED30001306

Overview
NameSMED30001306
Smed IDSMED30001306
Length (bp)1732
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30001306 (SMED30001306) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30001306 (SMED30001306) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30001306 (SMED30001306) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30001306 (SMED30001306) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30001306

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 28

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30001306SMESG000068156.1 SMESG000064685.1 SMESG000010609.1 SMESG000076470.1 SMESG000073857.1 SMESG000062429.1 SMESG000060487.1 SMESG000058575.1 SMESG000053533.1 SMESG000026676.1 SMESG000020635.1 SMESG000015751.1 SMESG000005260.1 Contig240newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30001306SMESG000049723.1 Contig240newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30001306SMESG000068156.1 SMESG000010609.1 SMESG000076470.1 SMESG000073857.1 SMESG000062429.1 SMESG000060487.1 SMESG000058575.1 SMESG000053533.1 SMESG000026676.1 SMESG000020635.1 SMESG000015751.1 SMESG000005260.1 Contig236newmark_estsPMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30001306SMESG000068156.1 SMESG000010609.1 SMESG000076470.1 SMESG000073857.1 SMESG000062429.1 SMESG000060487.1 SMESG000058575.1 SMESG000053533.1 SMESG000026676.1 SMESG000020635.1 SMESG000015751.1 SMESG000005260.1 Contig236newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30001306SMESG000068156.1 SMESG000064685.1 SMESG000043474.1 SMESG000010609.1 SMESG000076470.1 SMESG000073857.1 SMESG000062429.1 SMESG000060487.1 SMESG000058575.1 SMESG000053533.1 SMESG000020635.1 SMESG000015751.1 SMESG000005260.1 Contig233newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30001306SMESG000068156.1 SMESG000064685.1 SMESG000010609.1 SMESG000076470.1 SMESG000073857.1 SMESG000062429.1 SMESG000060487.1 SMESG000058575.1 SMESG000053533.1 SMESG000026676.1 SMESG000020635.1 SMESG000015751.1 SMESG000005260.1 Contig239newmark_estsPMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30001306SMESG000068156.1 SMESG000064685.1 SMESG000010609.1 SMESG000076470.1 SMESG000073857.1 SMESG000062429.1 SMESG000060487.1 SMESG000058575.1 SMESG000053533.1 SMESG000026676.1 SMESG000020635.1 SMESG000015751.1 SMESG000005260.1 Contig239newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30001306SMESG000057816.1 Contig239newmark_estsPMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30001306SMESG000057816.1 Contig239newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30001306SMESG000071252.1 Contig233newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30001306SMESG000037949.1 Contig236newmark_estsPMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30001306SMESG000037949.1 Contig236newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30001306SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30001306SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30001306SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30001306SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30001306SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30001306SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30001306SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30001306SMESG000060566.1 SMESG000060556.1 SMESG000060487.1 dd_Smed_v6_4002_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
oocyteSMED30001306SMESG000060487.1 Contig4435newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30001306SMESG000060487.1 Contig4435newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30001306SMESG000060487.1 Contig4435newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30001306SMESG000061766.1 Contig4435newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30001306SMESG000061766.1 Contig4435newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30001306SMESG000061766.1 Contig4435newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
epidermisSMED30001306SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30001306SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30001306 vs. Planmine SMEST
Match: SMESG000060556.1 (SMESG000060556.1)

HSP 1 Score: 771.155 bits (1990), Expect = 0.000e+0
Identity = 378/438 (86.30%), Postives = 393/438 (89.73%), Query Frame = 1
Query:   91 MDIYSEYHISSKESLSYLTRGIPERYEKHNQIEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCIRCRYRLIDLTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQARLWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMERCGIEISTECPKACVNDVCNAGVCHETYDRDPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKISWRAHSDDILGIWKEKISLKIQVKRNHLYFLYEEKYLFIKKTITNKYQPNQNDCVYLSFDANFIRIKFVQNDSTIITKLPVPKDLSKEDFTSISVLSGPGFIDIVHKLRPSQYQWLPFHRKFFVIKTD-------LSINASDSREEKINNSQVNVKDVLITILSVLLMISIIWIFYFLKNSRKMKIIRK 1383
            MDIYSEYHISSKESLSYLTRGIPERYE HNQ EFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGC+RCRYRLIDLTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQA+LWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMER GIEISTECPKACVNDVCNAGVCHETYDR+PFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIK SWRAHSDDILGIWKEKISLKIQVKRNHLYFLYEEK+LFIKKTITNKYQPNQNDCVYLSFD NFI IKFVQNDSTIITKLPVPKDLSKEDFTSIS+LS PGFIDIVHKLRPSQYQWLPF  KFFV +TD        S+     +  K  +S    ++++I ILS LL+I II     L     +KI RK
Sbjct:    1 MDIYSEYHISSKESLSYLTRGIPERYEIHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCVRCRYRLIDLTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQAKLWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDREPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKTSWRAHSDDILGIWKEKISLKIQVKRNHLYFLYEEKFLFIKKTITNKYQPNQNDCVYLSFDTNFIGIKFVQNDSTIITKLPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTDRSAIPKKTSMVLQTPQYSKRKDSGSISREIVIGILSFLLLIFII-----LAAVYAVKIFRK 433          
BLAST of SMED30001306 vs. Planmine SMEST
Match: SMESG000060556.1 (SMESG000060556.1)

HSP 1 Score: 765.763 bits (1976), Expect = 0.000e+0
Identity = 377/448 (84.15%), Postives = 397/448 (88.62%), Query Frame = 1
Query:   70 VFDSGDHMDIYSEYHISSKESLSYLTRGIPERYEKHNQIEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCIRCRYRLIDLTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQARLWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMERCGIEISTECPKACVNDVCNAGVCHETYDRDPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKISWRAHSDDILGIWKEKISLKIQVKRNHLYFLYEEKYLFIKKTITNKYQPNQNDCVYLSFDANFIRIKFVQNDSTIITKLPVPKDLSKEDFTSISVLSGPGFIDIVHKLRPSQYQWLPFHRKFFVIKTD-------LSINASDSREEKINNSQVNVKDVLITILSVLLMISIIWIFYFLKNSRKMKIIRKNSK 1392
            +F  G  + IY+EYHISSKESLSYLTRGIPERYE HNQ EFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGC+RCRYRLIDLTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQA+LWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMER GIEISTECPKACVNDVCNAGVCHETYDR+PFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIK SWRAHSDDILGIWKEKISLKIQVKRNHLYFLYEEK+LFIKKTITNKYQPNQNDCVYLSFD NFI IKFVQNDSTIITKLPVPKDLSKEDFTSIS+LS PGFIDIVHKLRPSQYQWLPF  KFFV +TD        S+     +  K  +S    ++++I ILS LL+I II     L     +KI RK ++
Sbjct:   18 IFIVGKQL-IYAEYHISSKESLSYLTRGIPERYEIHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCVRCRYRLIDLTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQAKLWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDREPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKTSWRAHSDDILGIWKEKISLKIQVKRNHLYFLYEEKFLFIKKTITNKYQPNQNDCVYLSFDTNFIGIKFVQNDSTIITKLPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTDRSAIPKKTSMVLQTPQYSKRKDSGSISREIVIGILSFLLLIFII-----LAAVYAVKIFRKRNE 459          
BLAST of SMED30001306 vs. Planmine SMEST
Match: SMESG000060556.1 (SMESG000060556.1)

HSP 1 Score: 764.222 bits (1972), Expect = 0.000e+0
Identity = 377/448 (84.15%), Postives = 397/448 (88.62%), Query Frame = 1
Query:   70 VFDSGDHMDIYSEYHISSKESLSYLTRGIPERYEKHNQIEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCIRCRYRLIDLTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQARLWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMERCGIEISTECPKACVNDVCNAGVCHETYDRDPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKISWRAHSDDILGIWKEKISLKIQVKRNHLYFLYEEKYLFIKKTITNKYQPNQNDCVYLSFDANFIRIKFVQNDSTIITKLPVPKDLSKEDFTSISVLSGPGFIDIVHKLRPSQYQWLPFHRKFFVIKTD-------LSINASDSREEKINNSQVNVKDVLITILSVLLMISIIWIFYFLKNSRKMKIIRKNSK 1392
            +F  G  + IY+EYHISSKESLSYLTRGIPERYE HNQ EFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGC+RCRYRLIDLTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQA+LWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMER GIEISTECPKACVNDVCNAGVCHETYDR+PFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIK SWRAHSDDILGIWKEKISLKIQVKRNHLYFLYEEK+LFIKKTITNKYQPNQNDCVYLSFD NFI IKFVQNDSTIITKLPVPKDLSKEDFTSIS+LS PGFIDIVHKLRPSQYQWLPF  KFFV +TD        S+     +  K  +S    ++++I ILS LL+I II     L     +KI RK ++
Sbjct:   18 IFIVGKQL-IYAEYHISSKESLSYLTRGIPERYEIHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCVRCRYRLIDLTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQAKLWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDREPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKTSWRAHSDDILGIWKEKISLKIQVKRNHLYFLYEEKFLFIKKTITNKYQPNQNDCVYLSFDTNFIGIKFVQNDSTIITKLPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTDRSAIPKKTSMVLQTPQYSKRKDSGSISREIVIGILSFLLLIFII-----LAAVYAVKIFRKRNE 459          
BLAST of SMED30001306 vs. Planmine SMEST
Match: SMESG000060566.1 (SMESG000060566.1)

HSP 1 Score: 761.14 bits (1964), Expect = 0.000e+0
Identity = 378/455 (83.08%), Postives = 395/455 (86.81%), Query Frame = 1
Query:   97 IYSEYHISSKESLSYLTRGIPERYEKHNQIEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCIRCRYRLIDLTVSITDRINIQCNNQPYKIFSYSNQKLINN----------------AYPIVMTFENSQARLWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMERCGIEISTECPKACVNDVCNAGVCHETYDRDPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKISWRAHSDDILGIWKEKISLKIQVKRNHLYFLYEEKYLFIKKTITNKYQPNQNDCVYLSFDANFIRIKFVQNDSTIITKLPVPKDLSKEDFTSISVLSGPGFIDIVHKLRPSQYQWLPFHRKFFVIKTDLSI----------NASDSREEKINNSQVNVKDVLITILSVLLMISIIWIFYF----LKNSRKMK 1371
            IY+EYHISSKES SYLTRGIPERYEKHNQIEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCIRCRYRLI+LTVSITDRINIQCNNQPYKIFSYSNQKLINN                 YPIVMTFENSQARLWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMERCGIEISTECPKACVNDVCNAGVCHETYDRDPFCDCTDSFLNGPFCNSSSITQMDSN+KYHSNTFRSKLIKISWRAHSDDILGIWKEKISLKIQVKRNHLY LYEEK LFIKKTITNKYQPNQNDCVYLSFDANFI IKFVQN+STIITKLPVPKDLSKEDFTSIS+LS PGFIDIVHKLRPSQYQWLPF  KFFV +TD S              DS+ +++++S    +D+ I ILS LL+I II    F     +N  +MK
Sbjct:   26 IYTEYHISSKESPSYLTRGIPERYEKHNQIEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCIRCRYRLIELTVSITDRINIQCNNQPYKIFSYSNQKLINNENKAFVVHLLWDSAVCTYPIVMTFENSQARLWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMERCGIEISTECPKACVNDVCNAGVCHETYDRDPFCDCTDSFLNGPFCNSSSITQMDSNKKYHSNTFRSKLIKISWRAHSDDILGIWKEKISLKIQVKRNHLYLLYEEKILFIKKTITNKYQPNQNDCVYLSFDANFIEIKFVQNNSTIITKLPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTDRSAIPKKTSIVLQTPQDSKRKRLSSS----RDIAIGILSFLLLIFIILAAVFAVKYFRNKNEMK 476          
BLAST of SMED30001306 vs. Planmine SMEST
Match: SMESG000060487.1 (SMESG000060487.1)

HSP 1 Score: 576.244 bits (1484), Expect = 0.000e+0
Identity = 288/391 (73.66%), Postives = 313/391 (80.05%), Query Frame = 1
Query:  292 MKIEFRGKEHHLTLYELCGCIRCRYRLIDLTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQARLWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMERCGIEISTECPKACVNDVCNAGVCHETYDRDPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKISWRAHSDDILGIWKEKISLKIQVKRNHLYFLYEEKY--LFIKKTITNKYQPNQNDCVYLSFDANFIRIKFVQNDSTIITKLPVPKDLSKEDFTSISVLSGPGFIDIVHKLRPSQYQWLPFHRKFFVIKTDLSINASDSREEKINNSQVNVKDVLITILSVLLMISIIWIFYFLKNSRKMKIIRKNSKSAPRDTQDCSMLNSSLMWCKTI 1458
            MKIEFRGKE+H TL ELCGC+ C+Y++IDL V ITDRINIQCN QP KI SYSNQK IN AYPI+      Q  LWLVNTP+HG  YPNP+QL GCIH++  NG P +ME  G  IST+CPK C NDVCNAGVC+ETYDR+PFCDCTDSFLNGP+CNSS    MD N KY   TFRSK IKISW+ HS  I GI     SL +   R+ LY LY  +    FI KTIT+KY P+Q   +Y +   N I IKFVQ + TI T  PVP+DLSKEDFTSISVLSGPGFIDIVHKLRPSQYQWLPFHRKFFVIKTDLSINASDSREEKINNSQVNVKDVLITILSVLLMISIIWIFYFLKNSRKMKIIRKNSKSAPRDTQDCSMLNSSLMWCKTI
Sbjct:    1 MKIEFRGKEYHSTLSELCGCVTCKYKIIDLRVVITDRINIQCNEQPNKICSYSNQKFINTAYPIMKMSGKFQVILWLVNTPSHGQHYPNPAQLGGCIHEISFNGKPLQMEIFGKAISTKCPKVCANDVCNAGVCNETYDREPFCDCTDSFLNGPYCNSSGSDGMDPNWKYPFETFRSKFIKISWQNHSGFIFGIGNMNTSLNVHSTRHCLYLLYHSRMEKFFINKTITDKYHPDQISRLYFTLGENII-IKFVQLNYTIFTNSPVPRDLSKEDFTSISVLSGPGFIDIVHKLRPSQYQWLPFHRKFFVIKTDLSINASDSREEKINNSQVNVKDVLITILSVLLMISIIWIFYFLKNSRKMKIIRKNSKSAPRDTQDCSMLNSSLMWCKTI 390          
The following BLAST results are available for this feature:
BLAST of SMED30001306 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001306 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001306 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001306 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001306 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001306 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001306 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001306 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001306 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001306 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001306 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001306 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001306 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001306 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000060556.10.000e+086.30SMESG000060556.1[more]
SMESG000060556.10.000e+084.15SMESG000060556.1[more]
SMESG000060556.10.000e+084.15SMESG000060556.1[more]
SMESG000060566.10.000e+083.08SMESG000060566.1[more]
SMESG000060487.10.000e+073.66SMESG000060487.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001306 ID=SMED30001306|Name=SMED30001306|organism=Schmidtea mediterranea sexual|type=transcript|length=1732bp
CATTAAATGTCTTATCGTTTCAATAGTAAAATCTAACAATTTCATGACAA
TATTTAAATTTCTGAAGCAGTCTTTGATTCCGGAGATCATATGGATATTT
ATTCCGAATACCACATCAGTAGCAAGGAGAGTCTCAGCTATTTGACCAGA
GGTATTCCAGAAAGATATGAAAAACATAATCAAATTGAATTTAAAATGAC
TCTAAATAGGATGGAAGGAACTTTATTATATATGTATGATAAATACGACA
ATCAATTGATAATTTCCCTTTCTGATGATCTGGAAGAGAGAATGAAAATA
GAATTCAGGGGTAAAGAACACCATTTAACTCTATATGAATTGTGTGGATG
CATCAGATGTAGATATCGATTGATCGATCTGACCGTGTCCATCACTGATC
GGATCAATATCCAATGCAATAATCAGCCATATAAAATATTCAGCTATTCC
AATCAGAAATTGATTAATAATGCTTATCCGATCGTGATGACATTTGAAAA
TTCCCAAGCGAGATTATGGCTTGTAAACACTCCTGCTCATGGTTACTTTT
ATCCGAACCCTTCGCAATTGGCTGGCTGCATTCATGATGTAATAATAAAT
GGAGTTCCTTCGGAAATGGAGAGATGTGGCATTGAGATATCCACTGAATG
TCCAAAGGCGTGCGTCAATGACGTTTGCAATGCTGGCGTGTGCCATGAAA
CATATGATAGAGATCCATTCTGTGATTGCACTGATTCATTCCTGAACGGC
CCATTCTGTAATTCAAGCAGCATCACCCAAATGGATTCCAATAGGAAATA
TCATTCAAATACTTTCCGGTCGAAATTAATCAAAATATCATGGCGGGCTC
ATTCTGATGATATTTTGGGCATTTGGAAAGAAAAGATTTCACTAAAGATT
CAAGTTAAAAGGAATCATCTTTACTTTCTATATGAAGAAAAATATTTATT
TATAAAAAAAACGATAACTAATAAATATCAACCAAATCAAAATGACTGCG
TTTATTTATCATTTGATGCAAACTTTATCAGAATAAAATTCGTACAAAAC
GATTCTACAATAATTACGAAACTACCTGTTCCCAAAGATTTATCTAAAGA
GGATTTCACATCAATCTCAGTGTTAAGTGGTCCTGGATTCATTGACATCG
TCCATAAATTGCGGCCCTCCCAATACCAATGGCTGCCGTTTCATCGCAAA
TTCTTTGTTATAAAAACTGATCTCTCCATCAATGCCAGCGATTCCAGAGA
AGAGAAGATAAATAATTCTCAAGTAAATGTTAAAGATGTCTTAATTACAA
TTTTGTCAGTTTTATTGATGATAAGCATCATATGGATTTTCTATTTTCTT
AAAAATTCCAGAAAAATGAAAATTATCAGGAAAAATTCTAAATCTGCTCC
AAGGGACACCCAAGATTGTTCAATGCTGAATAGTTCATTGATGTGGTGCA
AAACAATATGAAGAGTTTCTCTGTAAATTTTTATTTGCATTTTATTTGTT
ATTAAAAATCATGATTCGTATTCAACCTTGAATGTAGCGAAATGGATGTT
GTGATTAGCATCTCGAACCTAATTAGCATAGCTTTATATACATACATTTA
TACAGATTTTTATTCGTGCACAATGTTCATTGCGTTAAACACAGACATAT
GAAAAGATTGATAATATTTTTTTATACATTTTCTTACGAATTTTGTCATA
TTGCCATGTTTTACATTTTATGTCATTTTCAT
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protein sequence of SMED30001306-orf-1

>SMED30001306-orf-1 ID=SMED30001306-orf-1|Name=SMED30001306-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=457bp
MDIYSEYHISSKESLSYLTRGIPERYEKHNQIEFKMTLNRMEGTLLYMYD
KYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCIRCRYRLIDLTVS
ITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQARLWLVNTPAH
GYFYPNPSQLAGCIHDVIINGVPSEMERCGIEISTECPKACVNDVCNAGV
CHETYDRDPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKIS
WRAHSDDILGIWKEKISLKIQVKRNHLYFLYEEKYLFIKKTITNKYQPNQ
NDCVYLSFDANFIRIKFVQNDSTIITKLPVPKDLSKEDFTSISVLSGPGF
IDIVHKLRPSQYQWLPFHRKFFVIKTDLSINASDSREEKINNSQVNVKDV
LITILSVLLMISIIWIFYFLKNSRKMKIIRKNSKSAPRDTQDCSMLNSSL
MWCKTI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000074oocyte
PLANA:0000099neuron
PLANA:0000106ciliated epithelial cell
PLANA:0000231vitelline gland
PLANA:0002032epidermal cell
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR013320ConA-like_dom_sf