Protein BUD31-like protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30001291 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of Protein BUD31-like protein vs. Ensembl Human
Match: BUD31 (BUD31 homolog [Source:HGNC Symbol;Acc:HGNC:29629]) HSP 1 Score: 222.631 bits (566), Expect = 3.528e-75 Identity = 97/144 (67.36%), Postives = 122/144 (84.72%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PP+GWE IEPT++E +++MR+AE +PHEGKRKVE+LWPI RIHH++++YIFDL++KRK IS+ELYE+C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPK KLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYIFDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. Ensembl Human
Match: BUD31 (BUD31 homolog [Source:HGNC Symbol;Acc:HGNC:29629]) HSP 1 Score: 222.631 bits (566), Expect = 3.528e-75 Identity = 97/144 (67.36%), Postives = 122/144 (84.72%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PP+GWE IEPT++E +++MR+AE +PHEGKRKVE+LWPI RIHH++++YIFDL++KRK IS+ELYE+C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPK KLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYIFDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. Ensembl Human
Match: BUD31 (BUD31 homolog [Source:HGNC Symbol;Acc:HGNC:29629]) HSP 1 Score: 134.806 bits (338), Expect = 5.480e-41 Identity = 68/144 (47.22%), Postives = 84/144 (58.33%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PP+GWE IEPT++E +++MR+ ELYE+C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPK KLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKAPPDGWELIEPTLDELDQKMRE-----------------------------------------ELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG 103
BLAST of Protein BUD31-like protein vs. Ensembl Celegans
Match: bud-31 (pep chromosome:WBcel235:III:9694670:9695356:-1 gene:WBGene00007400.1 transcript:C07A9.2.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:bud-31) HSP 1 Score: 197.593 bits (501), Expect = 1.067e-65 Identity = 83/141 (58.87%), Postives = 110/141 (78.01%), Query Frame = 3 Query: 33 IRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 +RR + PPEGW+ IEPT+E+F +MR+AE +PHEGKRK E WPI RIHH+RS+Y++D+Y+K+ IS+ELYEFC+ +K+AD LIAKWKK GYENLCC+ C+ ++ T CICRVPK KL+ +++EC CGC GCSG Sbjct: 7 LRRVRKSPPEGWDLIEPTLEQFEAKMREAETEPHEGKRKTEINWPIFRIHHQRSRYVYDMYYKKAEISRELYEFCLTAKFADAALIAKWKKQGYENLCCVKCVNTRDSNFGTACICRVPKSKLDAERVIECVHCGCHGCSG 147
BLAST of Protein BUD31-like protein vs. Ensembl Celegans
Match: bud-31 (pep chromosome:WBcel235:III:9694668:9696517:-1 gene:WBGene00007400.1 transcript:C07A9.2.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:bud-31) HSP 1 Score: 197.593 bits (501), Expect = 1.067e-65 Identity = 83/141 (58.87%), Postives = 110/141 (78.01%), Query Frame = 3 Query: 33 IRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 +RR + PPEGW+ IEPT+E+F +MR+AE +PHEGKRK E WPI RIHH+RS+Y++D+Y+K+ IS+ELYEFC+ +K+AD LIAKWKK GYENLCC+ C+ ++ T CICRVPK KL+ +++EC CGC GCSG Sbjct: 7 LRRVRKSPPEGWDLIEPTLEQFEAKMREAETEPHEGKRKTEINWPIFRIHHQRSRYVYDMYYKKAEISRELYEFCLTAKFADAALIAKWKKQGYENLCCVKCVNTRDSNFGTACICRVPKSKLDAERVIECVHCGCHGCSG 147
BLAST of Protein BUD31-like protein vs. Ensembl Fly
Match: l(1)10Bb (gene:FBgn0001491 transcript:FBtr0073460) HSP 1 Score: 215.698 bits (548), Expect = 9.206e-73 Identity = 95/144 (65.97%), Postives = 121/144 (84.03%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M +RRS+ PPP+GWE IEPT+EE ++MR+AE +PHEGKR E+LWPI +IHH++++YI+DL+++RK IS+ELY++C++ K AD NLIAKWKK+GYENLCCL CIQ +T TNCICRVPK KLEEG+IVEC CGCRGCSG Sbjct: 1 MPKVRRSRKPPPDGWELIEPTLEELEQKMREAETEPHEGKRITESLWPIFKIHHQKTRYIYDLFYRRKAISRELYDYCLKEKIADGNLIAKWKKSGYENLCCLRCIQTRDTNFGTNCICRVPKCKLEEGRIVECVHCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. Ensembl Zebrafish
Match: bud31 (BUD31 homolog [Source:NCBI gene;Acc:445383]) HSP 1 Score: 228.794 bits (582), Expect = 8.208e-78 Identity = 100/144 (69.44%), Postives = 124/144 (86.11%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PPP+GWE IEPT++E +++MR+AE +PHEGKRKVE+LWPI R+HH+RS+YIFDL++KRK IS+ELYE+C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPKGKLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKPPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRLHHQRSRYIFDLFYKRKAISRELYEYCIREGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKGKLEVGRIIECTHCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. Ensembl Xenopus
Match: bud31 (BUD31 homolog [Source:NCBI gene;Acc:549113]) HSP 1 Score: 223.787 bits (569), Expect = 1.375e-75 Identity = 97/144 (67.36%), Postives = 123/144 (85.42%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PPP+GWE IEPT++E +++MR+AE +PHEGKRKVE+LWPI RIHH++++YIFDL++KRK IS+ELY++C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPK KLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKPPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYIFDLFYKRKAISRELYDYCIREGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKTKLEVGRIIECTHCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. Ensembl Xenopus
Match: bud31 (BUD31 homolog [Source:NCBI gene;Acc:549113]) HSP 1 Score: 197.978 bits (502), Expect = 1.349e-65 Identity = 85/127 (66.93%), Postives = 109/127 (85.83%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLE 404 M ++RS+ PPP+GWE IEPT++E +++MR+AE +PHEGKRKVE+LWPI RIHH++++YIFDL++KRK IS+ELY++C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPK KLE Sbjct: 1 MPKVKRSRKPPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYIFDLFYKRKAISRELYDYCIREGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKTKLE 127
BLAST of Protein BUD31-like protein vs. Ensembl Xenopus
Match: bud31 (BUD31 homolog [Source:NCBI gene;Acc:549113]) HSP 1 Score: 135.961 bits (341), Expect = 1.325e-41 Identity = 68/144 (47.22%), Postives = 85/144 (59.03%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PPP+GWE IEPT++E +++MR+ ELY++C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPK KLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKPPPDGWELIEPTLDELDQKMRE-----------------------------------------ELYDYCIREGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKTKLEVGRIIECTHCGCRGCSG 103
BLAST of Protein BUD31-like protein vs. Ensembl Mouse
Match: Bud31 (BUD31 homolog [Source:MGI Symbol;Acc:MGI:2141291]) HSP 1 Score: 222.631 bits (566), Expect = 2.422e-75 Identity = 97/144 (67.36%), Postives = 122/144 (84.72%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PP+GWE IEPT++E +++MR+AE +PHEGKRKVE+LWPI RIHH++++YIFDL++KRK IS+ELYE+C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPK KLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYIFDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. Ensembl Mouse
Match: Bud31 (BUD31 homolog [Source:MGI Symbol;Acc:MGI:2141291]) HSP 1 Score: 222.631 bits (566), Expect = 2.422e-75 Identity = 97/144 (67.36%), Postives = 122/144 (84.72%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PP+GWE IEPT++E +++MR+AE +PHEGKRKVE+LWPI RIHH++++YIFDL++KRK IS+ELYE+C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPK KLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYIFDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. Ensembl Mouse
Match: Bud31 (BUD31 homolog [Source:MGI Symbol;Acc:MGI:2141291]) HSP 1 Score: 222.631 bits (566), Expect = 2.422e-75 Identity = 97/144 (67.36%), Postives = 122/144 (84.72%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PP+GWE IEPT++E +++MR+AE +PHEGKRKVE+LWPI RIHH++++YIFDL++KRK IS+ELYE+C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPK KLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYIFDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. Ensembl Mouse
Match: Bud31 (BUD31 homolog [Source:MGI Symbol;Acc:MGI:2141291]) HSP 1 Score: 134.806 bits (338), Expect = 3.762e-41 Identity = 68/144 (47.22%), Postives = 84/144 (58.33%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PP+GWE IEPT++E +++MR+ ELYE+C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPK KLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKAPPDGWELIEPTLDELDQKMRE-----------------------------------------ELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG 103
BLAST of Protein BUD31-like protein vs. UniProt/SwissProt
Match: sp|Q567Z7|BUD31_DANRE (Protein BUD31 homolog OS=Danio rerio OX=7955 GN=bud31 PE=2 SV=1) HSP 1 Score: 226.868 bits (577), Expect = 3.501e-76 Identity = 98/144 (68.06%), Postives = 124/144 (86.11%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PPP+GWE +EPT++E +++MR+AE +PHEGKRKVE+LWPI R+HH+RS+YIFDL++KRK IS+ELY++C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPKGKLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKPPPDGWELVEPTLDELDQKMREAETEPHEGKRKVESLWPIFRLHHQRSRYIFDLFYKRKAISRELYKYCIRGGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKGKLEVGRIIECTHCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. UniProt/SwissProt
Match: sp|Q962X9|BUD31_BRABE (Protein BUD31 homolog OS=Branchiostoma belcheri OX=7741 PE=2 SV=1) HSP 1 Score: 226.483 bits (576), Expect = 5.486e-76 Identity = 101/144 (70.14%), Postives = 123/144 (85.42%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M +RRS+ PPPEGWE IEPT++E +++MR+AE +PHEGKRKVEALWPI +IHH++S+YIFDL+++RK IS+ELYE+C++ ADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPK KLEEG+IVEC CGCRGCSG Sbjct: 1 MPKVRRSRKPPPEGWELIEPTLDELDQKMREAETEPHEGKRKVEALWPIFKIHHQKSRYIFDLFYRRKAISRELYEYCLKEGIADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEEGRIVECVHCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. UniProt/SwissProt
Match: sp|P12805|BUD31_XENLA (Protein BUD31 homolog OS=Xenopus laevis OX=8355 GN=bud31 PE=2 SV=1) HSP 1 Score: 225.713 bits (574), Expect = 1.276e-75 Identity = 98/144 (68.06%), Postives = 123/144 (85.42%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PPP+GWE IEPT++E +++MR+AE DPHEGKRKVE+LWPI RIHH++++YIFDL++KRK IS+ELY++C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPK KLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKPPPDGWELIEPTLDELDQKMREAETDPHEGKRKVESLWPIFRIHHQKTRYIFDLFYKRKAISRELYDYCIREGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKTKLEVGRIIECTHCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. UniProt/SwissProt
Match: sp|O70454|BUD31_RAT (Protein BUD31 homolog OS=Rattus norvegicus OX=10116 GN=Bud31 PE=2 SV=2) HSP 1 Score: 222.631 bits (566), Expect = 1.694e-74 Identity = 97/144 (67.36%), Postives = 122/144 (84.72%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PP+GWE IEPT++E +++MR+AE +PHEGKRKVE+LWPI RIHH++++YIFDL++KRK IS+ELYE+C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPK KLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYIFDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. UniProt/SwissProt
Match: sp|Q6PGH1|BUD31_MOUSE (Protein BUD31 homolog OS=Mus musculus OX=10090 GN=Bud31 PE=1 SV=2) HSP 1 Score: 222.631 bits (566), Expect = 1.694e-74 Identity = 97/144 (67.36%), Postives = 122/144 (84.72%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PP+GWE IEPT++E +++MR+AE +PHEGKRKVE+LWPI RIHH++++YIFDL++KRK IS+ELYE+C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPK KLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYIFDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. TrEMBL
Match: A0A094ZLM0 (Protein BUD31-like protein (Fragment) OS=Schistosoma haematobium OX=6185 GN=MS3_02041 PE=4 SV=1) HSP 1 Score: 246.128 bits (627), Expect = 3.119e-81 Identity = 113/144 (78.47%), Postives = 125/144 (86.81%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 MAP RRSK PPPEGWE IEPTIEE NR+MR+AE DPHEGKRKVEA WPI RIHHKRS++I+DLY+KRK ISKELYEFC++ K AD NLIAKWKK GYENLCCL CIQ +T TNC+CRVPK KLEEGK+VECQ+CGCRGCSG Sbjct: 1 MAPYRRSKKPPPEGWELIEPTIEELNRKMREAETDPHEGKRKVEAEWPIFRIHHKRSRFIYDLYYKRKAISKELYEFCIKEKIADANLIAKWKKQGYENLCCLRCIQSRDTNFGTNCVCRVPKSKLEEGKVVECQSCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. TrEMBL
Match: A0A183K3N6 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS9604 PE=4 SV=1) HSP 1 Score: 246.128 bits (627), Expect = 3.119e-81 Identity = 113/144 (78.47%), Postives = 125/144 (86.81%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 MAP RRSK PPPEGWE IEPTIEE NR+MR+AE DPHEGKRKVEA WPI RIHHKRS++I+DLY+KRK ISKELYEFC++ K AD NLIAKWKK GYENLCCL CIQ +T TNC+CRVPK KLEEGK+VECQ+CGCRGCSG Sbjct: 1 MAPYRRSKKPPPEGWELIEPTIEELNRKMREAETDPHEGKRKVEAEWPIFRIHHKRSRFIYDLYYKRKAISKELYEFCIKEKIADANLIAKWKKQGYENLCCLRCIQSRDTNFGTNCVCRVPKSKLEEGKVVECQSCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. TrEMBL
Match: A0A183NSD1 (Uncharacterized protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS5017 PE=4 SV=1) HSP 1 Score: 246.128 bits (627), Expect = 3.119e-81 Identity = 113/144 (78.47%), Postives = 125/144 (86.81%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 MAP RRSK PPPEGWE IEPTIEE NR+MR+AE DPHEGKRKVEA WPI RIHHKRS++I+DLY+KRK ISKELYEFC++ K AD NLIAKWKK GYENLCCL CIQ +T TNC+CRVPK KLEEGK+VECQ+CGCRGCSG Sbjct: 1 MAPYRRSKKPPPEGWELIEPTIEELNRKMREAETDPHEGKRKVEAEWPIFRIHHKRSRFIYDLYYKRKAISKELYEFCIKEKIADANLIAKWKKQGYENLCCLRCIQSRDTNFGTNCVCRVPKSKLEEGKVVECQSCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. TrEMBL
Match: G4VHY6 (Putative g10 protein homolog OS=Schistosoma mansoni OX=6183 GN=Smp_092380 PE=4 SV=1) HSP 1 Score: 246.128 bits (627), Expect = 3.119e-81 Identity = 113/144 (78.47%), Postives = 125/144 (86.81%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 MAP RRSK PPPEGWE IEPTIEE NR+MR+AE DPHEGKRKVEA WPI RIHHKRS++I+DLY+KRK ISKELYEFC++ K AD NLIAKWKK GYENLCCL CIQ +T TNC+CRVPK KLEEGK+VECQ+CGCRGCSG Sbjct: 1 MAPYRRSKKPPPEGWELIEPTIEELNRKMREAETDPHEGKRKVEAEWPIFRIHHKRSRFIYDLYYKRKAISKELYEFCIKEKIADANLIAKWKKQGYENLCCLRCIQSRDTNFGTNCVCRVPKSKLEEGKVVECQSCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. TrEMBL
Match: A0A183MER8 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS14543 PE=4 SV=1) HSP 1 Score: 246.128 bits (627), Expect = 3.119e-81 Identity = 113/144 (78.47%), Postives = 125/144 (86.81%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 MAP RRSK PPPEGWE IEPTIEE NR+MR+AE DPHEGKRKVEA WPI RIHHKRS++I+DLY+KRK ISKELYEFC++ K AD NLIAKWKK GYENLCCL CIQ +T TNC+CRVPK KLEEGK+VECQ+CGCRGCSG Sbjct: 1 MAPYRRSKKPPPEGWELIEPTIEELNRKMREAETDPHEGKRKVEAEWPIFRIHHKRSRFIYDLYYKRKAISKELYEFCIKEKIADANLIAKWKKQGYENLCCLRCIQSRDTNFGTNCVCRVPKSKLEEGKVVECQSCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. Ensembl Cavefish
Match: bud31 (BUD31 homolog [Source:NCBI gene;Acc:103025586]) HSP 1 Score: 228.794 bits (582), Expect = 6.956e-78 Identity = 100/144 (69.44%), Postives = 124/144 (86.11%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PPP+GWE IEPT++E +++MR+AE +PHEGKRKVE+LWPI R+HH+RS+YIFDL++KRK IS+ELYE+C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPKGKLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKPPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRLHHQRSRYIFDLFYKRKAISRELYEYCIREGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKGKLEVGRIIECTHCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. Ensembl Yeast
Match: BUD31 (Component of the SF3b subcomplex of the U2 snRNP; increases efficiency of first and second step pre-mRNA splicing; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis [Source:SGD;Acc:S000000659]) HSP 1 Score: 155.606 bits (392), Expect = 1.104e-49 Identity = 78/150 (52.00%), Postives = 105/150 (70.00%), Query Frame = 3 Query: 36 RRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHE--GKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEE-----GKIV---ECQTCGCRGCSG 455 RRSK P P+G+E I+PT+ +F Q+RDA+ D + E LW IM++HH+RS+YI+ LY+KRK ISK+LY++ ++ KYADK LIAKW+K GYE LCCL CIQ++ T + CICRVP+ +LEE G V +C CGCRGC+ Sbjct: 7 RRSK-PAPDGFEKIKPTLTDFEIQLRDAQKDKSSKLAAKSNEQLWEIMQLHHQRSRYIYTLYYKRKAISKDLYDWLIKEKYADKLLIAKWRKTGYEKLCCLRCIQKNETNNGSTCICRVPRAQLEEEARKKGTQVSFHQCVHCGCRGCAS 155
BLAST of Protein BUD31-like protein vs. Ensembl Nematostella
Match: EDO36963 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SH57]) HSP 1 Score: 232.261 bits (591), Expect = 1.105e-79 Identity = 104/144 (72.22%), Postives = 123/144 (85.42%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M +RRS+ PPPEGWE IEPT++E +++MR+AE DPHEGKRKVEALWPI RIHH++S+Y++DLY+KRK ISKELY+FC++ +ADKNLIAKWKK GYENLCCL CIQ +T NCICRVPK KLEEGKIVEC CGCRGCSG Sbjct: 1 MPKVRRSRKPPPEGWELIEPTLDELDQKMREAETDPHEGKRKVEALWPIFRIHHQKSRYVYDLYYKRKAISKELYDFCLKEGHADKNLIAKWKKQGYENLCCLRCIQTHDTNFGANCICRVPKSKLEEGKIVECVVCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. Ensembl Medaka
Match: bud31 (BUD31 homolog [Source:NCBI gene;Acc:101174835]) HSP 1 Score: 224.557 bits (571), Expect = 4.121e-76 Identity = 97/144 (67.36%), Postives = 123/144 (85.42%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PPP+GWE IEPT++E +++MR+AE +PHEGKRKVE+LWPI R+HH+RS+YI+DL++KRK IS+ELY++C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPK KLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKPPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRLHHQRSRYIYDLFYKRKAISRELYDYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. Ensembl Medaka
Match: bud31 (BUD31 homolog [Source:NCBI gene;Acc:101174835]) HSP 1 Score: 136.732 bits (343), Expect = 4.637e-42 Identity = 68/144 (47.22%), Postives = 85/144 (59.03%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 M ++RS+ PPP+GWE IEPT++E +++MR+ ELY++C++ YADKNLIAKWKK GYENLCCL CIQ +T TNCICRVPK KLE G+I+EC CGCRGCSG Sbjct: 1 MPKVKRSRKPPPDGWELIEPTLDELDQKMRE-----------------------------------------ELYDYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG 103
BLAST of Protein BUD31-like protein vs. Planmine SMEST
Match: SMESG000018009.1 (SMESG000018009.1) HSP 1 Score: 301.212 bits (770), Expect = 1.345e-106 Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG Sbjct: 1 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 144
BLAST of Protein BUD31-like protein vs. Planmine SMEST
Match: SMESG000017986.1 (SMESG000017986.1) HSP 1 Score: 301.212 bits (770), Expect = 1.345e-106 Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 3 Query: 24 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 455 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG Sbjct: 1 MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMRIHHKRSKYIFDLYHKRKTISKELYEFCVQSKYADKNLIAKWKKNGYENLCCLACIQQSNTTMKTNCICRVPKGKLEEGKIVECQTCGCRGCSG 144 The following BLAST results are available for this feature:
BLAST of Protein BUD31-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 3
BLAST of Protein BUD31-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 2
BLAST of Protein BUD31-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 1
BLAST of Protein BUD31-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of Protein BUD31-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 3
BLAST of Protein BUD31-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 4
BLAST of Protein BUD31-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Protein BUD31-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Protein BUD31-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of Protein BUD31-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Protein BUD31-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Protein BUD31-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Protein BUD31-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 2
BLAST of Protein BUD31-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30001291 ID=SMED30001291|Name=Protein BUD31-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=502bpback to top protein sequence of SMED30001291-orf-1 >SMED30001291-orf-1 ID=SMED30001291-orf-1|Name=SMED30001291-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=145bp MAPIRRSKNPPPEGWESIEPTIEEFNRQMRDAEVDPHEGKRKVEALWPIMback to top Annotated Terms
The following terms have been associated with this transcript:
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