electron transfer flavoprotein subunit alpha, mitochondrial

Overview
Nameelectron transfer flavoprotein subunit alpha, mitochondrial
Smed IDSMED30001244
Length (bp)1139
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of electron transfer flavoprotein subunit alpha, mitochondrial (SMED30001244) t-SNE clustered cells

Violin plots show distribution of expression levels for electron transfer flavoprotein subunit alpha, mitochondrial (SMED30001244) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of electron transfer flavoprotein subunit alpha, mitochondrial (SMED30001244) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for electron transfer flavoprotein subunit alpha, mitochondrial (SMED30001244) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30001244

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior sideSMED30001244h1SMcG0004340 dd_Smed_v4_1889_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30001244h1SMcG0004340 dd_Smed_v4_1889_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Human
Match: ETFA (electron transfer flavoprotein subunit alpha [Source:HGNC Symbol;Acc:HGNC:3481])

HSP 1 Score: 344.354 bits (882), Expect = 6.012e-117
Identity = 189/317 (59.62%), Postives = 248/317 (78.23%), Query Frame = 2
Query:  134 IRFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALLK 1084
            +RF S+LVIA+H+   L PITLN +TAA++LG +V  LV G  C  VA++  KV G+  V +A + ++KG L E ++ L+   Q + N++HI  GA++ GKN +PR A+K+ ++ ISDII I+SP+TFVRTIYAGNAL TV+ +EKV   +VRGTSF+ A     S   +  S  + V  + ++++ +++ K+DRPEL GAK+V+SGGRGLKSG+NFK+LY+LAD+L+AAVGASRAAVDAG+V ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+G+V DLFK VPEM+ +LK
Sbjct:   17 LRFQSTLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPV--EISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILK 331          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Human
Match: ETFA (electron transfer flavoprotein subunit alpha [Source:HGNC Symbol;Acc:HGNC:3481])

HSP 1 Score: 295.434 bits (755), Expect = 2.024e-98
Identity = 165/273 (60.44%), Postives = 218/273 (79.85%), Query Frame = 2
Query:  266 KSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALLK 1084
            ++VA++  KV G+  V +A + ++KG L E ++ L+   Q + N++HI  GA++ GKN +PR A+K+ ++ ISDII I+SP+TFVRTIYAGNAL TV+ +EKV   +VRGTSF+ A     S   +  S  + V  + ++++ +++ K+DRPEL GAK+V+SGGRGLKSG+NFK+LY+LAD+L+AAVGASRAAVDAG+V ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+G+V DLFK VPEM+ +LK
Sbjct:   12 RAVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPV--EISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILK 282          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Human
Match: ETFA (electron transfer flavoprotein subunit alpha [Source:HGNC Symbol;Acc:HGNC:3481])

HSP 1 Score: 292.352 bits (747), Expect = 5.808e-97
Identity = 160/312 (51.28%), Postives = 215/312 (68.91%), Query Frame = 2
Query:  134 IRFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEM 1069
            +RF S+LVIA+H+   L PITLN +TAA++LG +V  LV G  C  VA++  KV G+  V +A + ++KG L E ++ L+   Q + N++HI  GA++ GKN +PR A+K+ ++ ISDII I+SP+TFVRTIYAGNAL TV+ +EKV   +VRGTSF+ A     S   +  S  + V  + ++++ +++ K+DRPEL GAK+V+SG    ++  +                       AG+V ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+G+V DLFK VPEM
Sbjct:   17 LRFQSTLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPV--EISEWLDQKLTKSDRPELTGAKVVVSGVGASRAAVD-----------------------AGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEM 303          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Human
Match: ETFA (electron transfer flavoprotein subunit alpha [Source:HGNC Symbol;Acc:HGNC:3481])

HSP 1 Score: 263.077 bits (671), Expect = 3.108e-86
Identity = 151/233 (64.81%), Postives = 191/233 (81.97%), Query Frame = 2
Query:  383 QSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALL 1081
            Q + N++HI  GA++ GKN +PR A+K+ ++ ISDII I+SP+TFVRTIYAGNAL TV+ +EKV   +VRGTSF+ A     S   +  S  + V  + ++++ +++ K+DRPEL GAK+V+SGGRGLKSG+NFK+LY+LAD+L+AAVGASRAAVDAG+V ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+G+V DLFK  P  +ALL
Sbjct:    4 QKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPV--EISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFK--PPWTALL 232          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Human
Match: ETFA (electron transfer flavoprotein subunit alpha [Source:HGNC Symbol;Acc:HGNC:3481])

HSP 1 Score: 254.218 bits (648), Expect = 3.756e-83
Identity = 145/216 (67.13%), Postives = 181/216 (83.80%), Query Frame = 2
Query:  437 NFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALLK 1084
            N +PR A+K+ ++ ISDII I+SP+TFVRTIYAGNAL TV+ +EKV   +VRGTSF+ A     S   +  S  + V  + ++++ +++ K+DRPEL GAK+V+SGGRGLKSG+NFK+LY+LAD+L+AAVGASRAAVDAG+V ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+G+V DLFK VPEM+ +LK
Sbjct:   14 NLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPV--EISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILK 227          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Celegans
Match: F27D4.1 (Probable electron transfer flavoprotein subunit alpha, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:Q93615])

HSP 1 Score: 323.939 bits (829), Expect = 2.125e-109
Identity = 183/315 (58.10%), Postives = 242/315 (76.83%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALL 1081
            R  S+LV+A+H + KL PITLN +TAASKLGN+V  LV G N   VAE+ +KV GVK V +A +   K  L E ++ ++   Q + NF+ I  G+++ G+  +PR A+K+ +S ISD+  + S ++F RT+YAGNA++ V+S   +  +T RGTSFEPA  E  S  ++  + +A++K D ++F+ +E+ K++RP+LA AK+V+SGGRGLKSGDNFK++Y+LADKL A VGASRAAVDAGYV ND+QVGQTGKIVAP+LYIA+GISGAIQHLAGMKDSKVIVAINKDP+APIFQVAD GL  DLFKAVPE++A L
Sbjct:   19 RLNSTLVVAEHDETKLAPITLNAITAASKLGNEVSVLVTGANATKVAEQVAKVNGVKRVLVAQDEKLKNNLPERVAPVILASQKQFNFTAITAGSSAFGRGVIPRVAAKLDVSSISDVTEVHSADSFTRTLYAGNAVKKVKSTAPIKLLTFRGTSFEPAK-EGGSGAVEN-APSADIKTDLSEFLGQELSKSERPDLATAKVVVSGGRGLKSGDNFKLIYDLADKLGAGVGASRAAVDAGYVPNDMQVGQTGKIVAPELYIAIGISGAIQHLAGMKDSKVIVAINKDPDAPIFQVADIGLKADLFKAVPELTAAL 331          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Fly
Match: wal (gene:FBgn0010516 transcript:FBtr0088082)

HSP 1 Score: 335.88 bits (860), Expect = 5.815e-114
Identity = 190/315 (60.32%), Postives = 244/315 (77.46%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALL 1081
            R  S+LV+A+H+   LNPITLN ++AA K+G DV  LV G  C   +E  SKV GV  V +A+N+ F G  AES++ L+   QS+  F+HIL GA++ GKN +PR A+K+ +S IS+II ++S +TFVRTIYAGNA+ T++S++ V  ITVR T+F PA     S  ++  + A +  +  ++F+ +E+ K+DRPELAGAK+++SGGRGLKSGDNFK+LY+LADK  AAVGASRAAVDAG+V NDLQ+GQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD G+V DLFKAVPE++  L
Sbjct:   17 RCKSTLVVAEHNNEVLNPITLNTISAAKKIGGDVTVLVAGTKCGPASEALSKVDGVTKVLVAENAAFNGFTAESLTPLVLAAQSQFKFTHILAGASAFGKNVLPRVAAKLDVSPISEIIDVKSDDTFVRTIYAGNAILTLKSKDAVKVITVRSTNFPPAATSGGSGAVEQ-APAGDFASSLSEFVSQELTKSDRPELAGAKVIVSGGRGLKSGDNFKLLYDLADKFGAAVGASRAAVDAGFVPNDLQIGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADIGIVADLFKAVPELTGKL 330          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Fly
Match: wal (gene:FBgn0010516 transcript:FBtr0088083)

HSP 1 Score: 335.88 bits (860), Expect = 5.815e-114
Identity = 190/315 (60.32%), Postives = 244/315 (77.46%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALL 1081
            R  S+LV+A+H+   LNPITLN ++AA K+G DV  LV G  C   +E  SKV GV  V +A+N+ F G  AES++ L+   QS+  F+HIL GA++ GKN +PR A+K+ +S IS+II ++S +TFVRTIYAGNA+ T++S++ V  ITVR T+F PA     S  ++  + A +  +  ++F+ +E+ K+DRPELAGAK+++SGGRGLKSGDNFK+LY+LADK  AAVGASRAAVDAG+V NDLQ+GQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD G+V DLFKAVPE++  L
Sbjct:   17 RCKSTLVVAEHNNEVLNPITLNTISAAKKIGGDVTVLVAGTKCGPASEALSKVDGVTKVLVAENAAFNGFTAESLTPLVLAAQSQFKFTHILAGASAFGKNVLPRVAAKLDVSPISEIIDVKSDDTFVRTIYAGNAILTLKSKDAVKVITVRSTNFPPAATSGGSGAVEQ-APAGDFASSLSEFVSQELTKSDRPELAGAKVIVSGGRGLKSGDNFKLLYDLADKFGAAVGASRAAVDAGFVPNDLQIGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADIGIVADLFKAVPELTGKL 330          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Zebrafish
Match: etfa (electron transfer flavoprotein subunit alpha [Source:ZFIN;Acc:ZDB-GENE-030131-4449])

HSP 1 Score: 357.836 bits (917), Expect = 2.206e-122
Identity = 201/315 (63.81%), Postives = 249/315 (79.05%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALL 1081
            RF S+LV+A+H+   L PITLN +TAA+KLG+DV  LV G NC  VAE+ SKV+GVK V +A +  +KG L E ++ L+   Q + +F+HI  GA++ GKN +PR A+K+ ++ ISDII I+SP+TFVRTIYAGNAL TV+  E V   TVRGTSFEPA  +  S   + VS +A V    ++++++ + K+DRPEL  AK+V+SGGRGLKSGDNFK+LY+LADKLNAAVGASRAAVDAGYV ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+GLV DLFK VPE++  L
Sbjct:   19 RFQSTLVVAEHNNETLTPITLNAITAATKLGSDVSCLVAGTNCAKVAEQLSKVQGVKKVLVAQHEAYKGLLPEELTPLILATQKQFSFTHICAGASAFGKNLLPRVAAKLDVAPISDIIEIKSPDTFVRTIYAGNALSTVKCNESVKVFTVRGTSFEPAAADGGSSTSEEVSPSAPV--GVSEWLEQTLSKSDRPELTSAKVVVSGGRGLKSGDNFKLLYDLADKLNAAVGASRAAVDAGYVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGLVADLFKVVPELTEAL 331          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Xenopus
Match: kif7 (kinesin family member 7 [Source:Xenbase;Acc:XB-GENE-6034962])

HSP 1 Score: 348.977 bits (894), Expect = 7.615e-119
Identity = 192/316 (60.76%), Postives = 248/316 (78.48%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALLK 1084
            RF S+LVIA+H   ++ P+TL+ +TAA +LG DV  LV G NC  VAEE +KV+GV  +++A +  +KG L E ++ L+ + Q K NF+HI  GA++ GK+ +PR A+K+ ++ +SD+I I+SP+TFVRTIYAGNAL TV+ EE V   ++RGTSFE A     S    TV+ +A      ++++ + + K+DRPEL  AK+V+SGGRGLKSG+NFK+LY+LAD+LNAAVGASRAAVDAG+V ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+GLV DLFKAVPEM+ +LK
Sbjct:   18 RFLSALVIAEHDTQQVTPVTLSAITAAKRLGADVSCLVAGTNCSKVAEELAKVQGVSKLFVAQHESYKGFLPEELTPLVLEAQKKFNFTHICAGASAFGKSLLPRIAAKLDVAPVSDVIEIKSPDTFVRTIYAGNALCTVKCEESVKVFSIRGTSFEAAETSGGSASSDTVAPSAPA--GLSEWLHQSLTKSDRPELTSAKVVVSGGRGLKSGENFKLLYDLADQLNAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGLVADLFKAVPEMTEILK 331          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Xenopus
Match: kif7 (kinesin family member 7 [Source:Xenbase;Acc:XB-GENE-6034962])

HSP 1 Score: 335.495 bits (859), Expect = 1.207e-113
Identity = 188/315 (59.68%), Postives = 244/315 (77.46%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFK--AVPEMSA 1075
            RF S+LVIA+H   ++ P+TL+ +TAA +LG DV  LV G NC  VAEE +KV+GV  +++A +  +KG L E ++ L+ + Q K NF+HI  GA++ GK+ +PR A+K+ ++ +SD+I I+SP+TFVRTIYAGNAL TV+ EE V   ++RGTSFE A     S    TV+ +A      ++++ + + K+DRPEL  AK+V+SGGRGLKSG+NFK+LY+LAD+LNAAVGASRAAVDAG+V ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+GLV DLFK   VP +S+
Sbjct:    9 RFLSALVIAEHDTQQVTPVTLSAITAAKRLGADVSCLVAGTNCSKVAEELAKVQGVSKLFVAQHESYKGFLPEELTPLVLEAQKKFNFTHICAGASAFGKSLLPRIAAKLDVAPVSDVIEIKSPDTFVRTIYAGNALCTVKCEESVKVFSIRGTSFEAAETSGGSASSDTVAPSAPA--GLSEWLHQSLTKSDRPELTSAKVVVSGGRGLKSGENFKLLYDLADQLNAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGLVADLFKREMVPAISS 321          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Xenopus
Match: kif7 (kinesin family member 7 [Source:Xenbase;Acc:XB-GENE-6034962])

HSP 1 Score: 335.109 bits (858), Expect = 2.382e-113
Identity = 185/306 (60.46%), Postives = 239/306 (78.10%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFK 1054
            RF S+LVIA+H   ++ P+TL+ +TAA +LG DV  LV G NC  VAEE +KV+GV  +++A +  +KG L E ++ L+ + Q K NF+HI  GA++ GK+ +PR A+K+ ++ +SD+I I+SP+TFVRTIYAGNAL TV+ EE V   ++RGTSFE A     S    TV+ +A      ++++ + + K+DRPEL  AK+V+SGGRGLKSG+NFK+LY+LAD+LNAAVGASRAAVDAG+V ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+GLV DLFK
Sbjct:   18 RFLSALVIAEHDTQQVTPVTLSAITAAKRLGADVSCLVAGTNCSKVAEELAKVQGVSKLFVAQHESYKGFLPEELTPLVLEAQKKFNFTHICAGASAFGKSLLPRIAAKLDVAPVSDVIEIKSPDTFVRTIYAGNALCTVKCEESVKVFSIRGTSFEAAETSGGSASSDTVAPSAPA--GLSEWLHQSLTKSDRPELTSAKVVVSGGRGLKSGENFKLLYDLADQLNAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGLVADLFK 321          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Xenopus
Match: kif7 (kinesin family member 7 [Source:Xenbase;Acc:XB-GENE-6034962])

HSP 1 Score: 333.954 bits (855), Expect = 4.826e-113
Identity = 185/306 (60.46%), Postives = 239/306 (78.10%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFK 1054
            RF S+LVIA+H   ++ P+TL+ +TAA +LG DV  LV G NC  VAEE +KV+GV  +++A +  +KG L E ++ L+ + Q K NF+HI  GA++ GK+ +PR A+K+ ++ +SD+I I+SP+TFVRTIYAGNAL TV+ EE V   ++RGTSFE A     S    TV+ +A      ++++ + + K+DRPEL  AK+V+SGGRGLKSG+NFK+LY+LAD+LNAAVGASRAAVDAG+V ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+GLV DLFK
Sbjct:   22 RFLSALVIAEHDTQQVTPVTLSAITAAKRLGADVSCLVAGTNCSKVAEELAKVQGVSKLFVAQHESYKGFLPEELTPLVLEAQKKFNFTHICAGASAFGKSLLPRIAAKLDVAPVSDVIEIKSPDTFVRTIYAGNALCTVKCEESVKVFSIRGTSFEAAETSGGSASSDTVAPSAPA--GLSEWLHQSLTKSDRPELTSAKVVVSGGRGLKSGENFKLLYDLADQLNAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGLVADLFK 325          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Xenopus
Match: kif7 (kinesin family member 7 [Source:Xenbase;Acc:XB-GENE-6034962])

HSP 1 Score: 333.954 bits (855), Expect = 5.559e-113
Identity = 185/306 (60.46%), Postives = 239/306 (78.10%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFK 1054
            RF S+LVIA+H   ++ P+TL+ +TAA +LG DV  LV G NC  VAEE +KV+GV  +++A +  +KG L E ++ L+ + Q K NF+HI  GA++ GK+ +PR A+K+ ++ +SD+I I+SP+TFVRTIYAGNAL TV+ EE V   ++RGTSFE A     S    TV+ +A      ++++ + + K+DRPEL  AK+V+SGGRGLKSG+NFK+LY+LAD+LNAAVGASRAAVDAG+V ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+GLV DLFK
Sbjct:   22 RFLSALVIAEHDTQQVTPVTLSAITAAKRLGADVSCLVAGTNCSKVAEELAKVQGVSKLFVAQHESYKGFLPEELTPLVLEAQKKFNFTHICAGASAFGKSLLPRIAAKLDVAPVSDVIEIKSPDTFVRTIYAGNALCTVKCEESVKVFSIRGTSFEAAETSGGSASSDTVAPSAPA--GLSEWLHQSLTKSDRPELTSAKVVVSGGRGLKSGENFKLLYDLADQLNAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGLVADLFK 325          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Mouse
Match: Etfa (electron transferring flavoprotein, alpha polypeptide [Source:MGI Symbol;Acc:MGI:106092])

HSP 1 Score: 333.569 bits (854), Expect = 6.468e-113
Identity = 187/317 (58.99%), Postives = 245/317 (77.29%), Query Frame = 2
Query:  134 IRFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALLK 1084
            +RF S+LVIA+H+   L PITLN +TAA +LG +V  LV G  C  V ++  KV GV  V +A +  +KG L E ++ L+ + Q + +++HI  GA++ GKN +PR A+K+ ++ +SDII I+SP+TFVRTIYAGNAL TV+ +EKV   +VRGTSFE A     S   +    ++ V    ++++ +++ K+DRPEL GAK+V+SGGRGLKSG+NFK+LY+LAD+L+AAVGASRAAVDAG+V ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+G+V DLFK VPEM+ +LK
Sbjct:   17 LRFQSTLVIAEHANDSLAPITLNTITAAGRLGGEVSCLVAGTKCDKVVQDLCKVAGVAKVLVAQHDAYKGLLPEELTPLILETQKQFSYTHICAGASAFGKNLLPRVAAKLNVAPVSDIIEIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFEAAATSGGSASSEKAPSSSSV--GISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILK 331          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. UniProt/SwissProt
Match: sp|Q8HXY0|ETFA_MACFA (Electron transfer flavoprotein subunit alpha, mitochondrial OS=Macaca fascicularis OX=9541 GN=ETFA PE=2 SV=1)

HSP 1 Score: 344.354 bits (882), Expect = 2.646e-116
Identity = 189/317 (59.62%), Postives = 248/317 (78.23%), Query Frame = 2
Query:  134 IRFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALLK 1084
            +RF S+LVIA+H+   L PITLN +TAA++LG +V  LV G  C  VA++  KV G+  V +A +  ++G LAE ++ L+   Q + N++HI  GA++ GKN +PR A+K+ ++ ISDII I+SP+TFVRTIYAGNAL TV+ +EKV   +VRGTSFE A     +   +  S  + V  + ++++ +++ K+DRPEL GAK+V+SGGRGLKSG+NFK+LY+LAD+L+AAVGASRAAVDAG+V ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+G+V DLFK VPEM+ +LK
Sbjct:   17 LRFQSTLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDAYRGLLAEDLTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFEAAATSGGTASSEKASSTSPV--EISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILK 331          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. UniProt/SwissProt
Match: sp|P13804|ETFA_HUMAN (Electron transfer flavoprotein subunit alpha, mitochondrial OS=Homo sapiens OX=9606 GN=ETFA PE=1 SV=1)

HSP 1 Score: 344.354 bits (882), Expect = 2.887e-116
Identity = 189/317 (59.62%), Postives = 248/317 (78.23%), Query Frame = 2
Query:  134 IRFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALLK 1084
            +RF S+LVIA+H+   L PITLN +TAA++LG +V  LV G  C  VA++  KV G+  V +A + ++KG L E ++ L+   Q + N++HI  GA++ GKN +PR A+K+ ++ ISDII I+SP+TFVRTIYAGNAL TV+ +EKV   +VRGTSF+ A     S   +  S  + V  + ++++ +++ K+DRPEL GAK+V+SGGRGLKSG+NFK+LY+LAD+L+AAVGASRAAVDAG+V ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+G+V DLFK VPEM+ +LK
Sbjct:   17 LRFQSTLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPV--EISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILK 331          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. UniProt/SwissProt
Match: sp|Q5RC31|ETFA_PONAB (Electron transfer flavoprotein subunit alpha, mitochondrial OS=Pongo abelii OX=9601 GN=ETFA PE=2 SV=1)

HSP 1 Score: 342.813 bits (878), Expect = 1.102e-115
Identity = 188/317 (59.31%), Postives = 247/317 (77.92%), Query Frame = 2
Query:  134 IRFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALLK 1084
            +RF S+LVIA+H+   L PITLN +TAA++LG +V  LV G  C  VA++  KV G+  V +A + +++G L E ++ L+   Q + N++HI  GA++ GKN +PR A+K+ ++ ISDII I+SP+TFVRTIYAGNAL TV+ +EKV   +VRGTSFE A     S   +  S  + V  + ++++ +++ K+DRPEL GAK+V+SGGRGLKSG+NFK+LY+LAD+ +AAVGASRAAVDAG+V ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+G+V DLFK VPEM+ +LK
Sbjct:   17 LRFQSTLVIAEHADDSLAPITLNTITAATRLGGEVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDVYRGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFEAAATSGGSASSEKASSTSPV--EISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQRHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILK 331          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. UniProt/SwissProt
Match: sp|Q2KJE4|ETFA_BOVIN (Electron transfer flavoprotein subunit alpha, mitochondrial OS=Bos taurus OX=9913 GN=ETFA PE=2 SV=1)

HSP 1 Score: 335.495 bits (859), Expect = 1.040e-112
Identity = 192/317 (60.57%), Postives = 243/317 (76.66%), Query Frame = 2
Query:  134 IRFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALLK 1084
            +RF S+LVIA+H+   L PITLN +TAA  LG +V  LV G  C  VA++  KV GV  V +A +  +KG L E ++ L+   Q + N +HI  GA++ GKN +PR A+K+ ++ ISDII I+SP+TFVRTIYAGNA+ TV+ +EKV   +VRGTSFE A     S   +  S  + V    ++++ +++ K+DRPEL GAK+V+SGGRGLKSG+NFK+LY+LAD+L+AAVGASRAAVDAG+V NDLQVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+G+V DLFK VPEM+ LLK
Sbjct:   17 LRFQSTLVIAEHANDTLAPITLNTITAAKHLGGEVSCLVAGTKCDKVAQDLCKVAGVAKVLVAQHDAYKGLLPEELTPLILATQKQFNHTHICAGASAFGKNLLPRIAAKLDVAPISDIIAIKSPDTFVRTIYAGNAICTVKCDEKVKVFSVRGTSFEAAAASGGSASSEKASSTSPV--GISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVTNDLQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTELLK 331          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. UniProt/SwissProt
Match: sp|Q99LC5|ETFA_MOUSE (Electron transfer flavoprotein subunit alpha, mitochondrial OS=Mus musculus OX=10090 GN=Etfa PE=1 SV=2)

HSP 1 Score: 333.569 bits (854), Expect = 4.524e-112
Identity = 187/317 (58.99%), Postives = 245/317 (77.29%), Query Frame = 2
Query:  134 IRFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALLK 1084
            +RF S+LVIA+H+   L PITLN +TAA +LG +V  LV G  C  V ++  KV GV  V +A +  +KG L E ++ L+ + Q + +++HI  GA++ GKN +PR A+K+ ++ +SDII I+SP+TFVRTIYAGNAL TV+ +EKV   +VRGTSFE A     S   +    ++ V    ++++ +++ K+DRPEL GAK+V+SGGRGLKSG+NFK+LY+LAD+L+AAVGASRAAVDAG+V ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+G+V DLFK VPEM+ +LK
Sbjct:   17 LRFQSTLVIAEHANDSLAPITLNTITAAGRLGGEVSCLVAGTKCDKVVQDLCKVAGVAKVLVAQHDAYKGLLPEELTPLILETQKQFSYTHICAGASAFGKNLLPRVAAKLNVAPVSDIIEIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFEAAATSGGSASSEKAPSSSSV--GISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILK 331          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. TrEMBL
Match: A0A4W5NNI5 (Electron transfer flavoprotein subunit alpha OS=Hucho hucho OX=62062 PE=4 SV=1)

HSP 1 Score: 368.237 bits (944), Expect = 4.090e-123
Identity = 205/315 (65.08%), Postives = 249/315 (79.05%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALL 1081
            RF S+LV+A+H+  KL PITLN +TAA KLG DV  LV G +C  VA+E SKV GVK V +A N  +KG L E+++ L+   Q + NF+HI  GA++ GKN +PR A+K+ ++ ISDII I+SP+TFVRTIYAGNAL TV+S + V   TVRGTSFEPA +E  S   + V  AA      +++++  + K+DRPEL  AK+V+SGGRGLKSGDNFK+LY+LADK+NAAVGASRAAVDAGYV ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+GLV DLFKAVPEM+A L
Sbjct:   19 RFQSTLVVAEHNNDKLTPITLNAITAAKKLGGDVSCLVAGTDCTKVAQEISKVLGVKTVLIAQNDAYKGSLPEALTPLILATQKQFNFTHICAGASAFGKNLLPRLAAKLDVAPISDIIEIKSPDTFVRTIYAGNALSTVKSNDNVKVFTVRGTSFEPAEVEGGSATSEEV--AASAPAGMSEWLEASLTKSDRPELTSAKVVVSGGRGLKSGDNFKLLYDLADKMNAAVGASRAAVDAGYVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGLVADLFKAVPEMTAAL 331          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. TrEMBL
Match: B5X7Q6 (electron transfer flavoprotein subunit alpha, mitochondrial OS=Salmo salar OX=8030 GN=ETFA PE=2 SV=1)

HSP 1 Score: 367.851 bits (943), Expect = 4.713e-123
Identity = 205/315 (65.08%), Postives = 249/315 (79.05%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALL 1081
            RF S+LV+A+H+  KL PITLN +TAA KLG DV  LV G +C  VA+E SKV GVK V +A N  +KG L E+++ L+   Q + NF+HI  GA++ GKN +PR A+K+ ++ ISDII I+SP+TFVRTIYAGNAL TV+S + V   TVRGTSFEPA +E  S   + V  AA      +++++  + K+DRPEL  AK+V+SGGRGLKSGDNFK+LY+LADK+NAAVGASRAAVDAGYV ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+GLV DLFKAVPEM+A L
Sbjct:   19 RFQSTLVVAEHNNDKLTPITLNAITAAKKLGGDVSCLVAGTDCTKVAQEISKVLGVKTVLIAQNDAYKGSLPEALTPLILATQKQFNFTHICAGASAFGKNLLPRLAAKLDVAPISDIIEIKSPDTFVRTIYAGNALSTVKSNDNVKVFTVRGTSFEPAEVEGGSATSEEV--AASTPAGMSEWLEASLTKSDRPELTSAKVVVSGGRGLKSGDNFKLLYDLADKMNAAVGASRAAVDAGYVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGLVADLFKAVPEMTAAL 331          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. TrEMBL
Match: B5RI57 (Electron-transfer-flavoprotein alpha polypeptide (Fragment) OS=Salmo salar OX=8030 GN=etfa PE=2 SV=1)

HSP 1 Score: 367.851 bits (943), Expect = 5.306e-123
Identity = 205/315 (65.08%), Postives = 249/315 (79.05%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALL 1081
            RF S+LV+A+H+  KL PITLN +TAA KLG DV  LV G +C  VA+E SKV GVK V +A N  +KG L E+++ L+   Q + NF+HI  GA++ GKN +PR A+K+ ++ ISDII I+SP+TFVRTIYAGNAL TV+S + V   TVRGTSFEPA +E  S   + V  AA      +++++  + K+DRPEL  AK+V+SGGRGLKSGDNFK+LY+LADK+NAAVGASRAAVDAGYV ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+GLV DLFKAVPEM+A L
Sbjct:   18 RFQSTLVVAEHNNDKLTPITLNAITAAKKLGGDVSCLVAGTDCTKVAQEISKVLGVKTVLIAQNDAYKGSLPEALTPLILATQKQFNFTHICAGASAFGKNLLPRLAAKLDVAPISDIIEIKSPDTFVRTIYAGNALSTVKSNDNVKVFTVRGTSFEPAEVEGGSATSEEV--AASTPAGMSEWLEASLTKSDRPELTSAKVVVSGGRGLKSGDNFKLLYDLADKMNAAVGASRAAVDAGYVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGLVADLFKAVPEMTAAL 330          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. TrEMBL
Match: A0A060WFG6 (ETF domain-containing protein OS=Oncorhynchus mykiss OX=8022 GN=GSONMT00068790001 PE=4 SV=1)

HSP 1 Score: 367.851 bits (943), Expect = 5.372e-123
Identity = 204/315 (64.76%), Postives = 250/315 (79.37%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALL 1081
            RF S+LV+A+H+  KL PITLN +TAA KLG DV  LV G +C  VA+E SKV GVK V +A N  +KG L E+++ L+   Q + NF+HI  GA++ GKN +PR A+K+ ++ ISDII I+SP+TFVRTIYAGNAL TV+S + V   TVRGTSFEPA +E  S   + V  AA   +  +++++  + K+DRPEL  AK+V+SGGRGLKSGDNFK+LY+LAD++NAAVGASRAAVDAGYV ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+GLV DLFKAVPEM+A L
Sbjct:   19 RFQSTLVVAEHNNDKLTPITLNAITAAKKLGGDVSCLVAGTDCTKVAQEISKVLGVKTVLIAQNDAYKGSLPEALTPLILATQKQFNFTHICAGASAFGKNLLPRLAAKLDVAPISDIIEIKSPDTFVRTIYAGNALSTVKSNDNVKVFTVRGTSFEPAEVEGGSATSEEV--AASTPDGMSEWLEASLTKSDRPELTSAKVVVSGGRGLKSGDNFKLLYDLADQMNAAVGASRAAVDAGYVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGLVADLFKAVPEMTAAL 331          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. TrEMBL
Match: A0A1S3IHU0 (electron transfer flavoprotein subunit alpha, mitochondrial OS=Lingula unguis OX=7574 GN=LOC106164432 PE=4 SV=1)

HSP 1 Score: 366.311 bits (939), Expect = 2.178e-122
Identity = 205/316 (64.87%), Postives = 253/316 (80.06%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALLK 1084
            RF S+LV+A+H    L PITLN V+AA ++G DV  LV G  C  VAEE SK+ GVK V +AD+  FKG + E+++ LL   Q + NF+HIL GAT+ GKN MPR A+K+ +S +SDIIG++SP+TFVRTIYAGNA+QTV+S++K+  +TVRGTSFEPA     S   +TVS  A+V N+ + ++  E+ K+DRPELAGA  V+SGGRG+K+G+NF++LY LAD + AAVGASRAAVDAG+V ND+QVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVAD+GLV DLFKAVPEM+  LK
Sbjct:   22 RFQSTLVVAEHDNESLYPITLNAVSAAKQIGEDVSCLVAGTQCAKVAEEVSKIAGVKKVLVADHDAFKGYMPEALTPLLLSTQQQFNFTHILAGATAFGKNLMPRLAAKLDVSPVSDIIGVKSPDTFVRTIYAGNAIQTVKSKDKIVVLTVRGTSFEPAETSGGSAGQETVS--ADVSNELSSYVGSEIHKSDRPELAGAATVVSGGRGMKNGENFQLLYNLADTMQAAVGASRAAVDAGFVPNDMQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADYGLVADLFKAVPEMTEKLK 335          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Cavefish
Match: etfa (electron transfer flavoprotein subunit alpha [Source:NCBI gene;Acc:103023592])

HSP 1 Score: 361.688 bits (927), Expect = 5.409e-124
Identity = 204/316 (64.56%), Postives = 248/316 (78.48%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALLK 1084
            RF S+LV+A+H+ G L PITLN +TAASKLG+DV  LV G  C  VAEE SKVKG+K + +A +  +KG L E ++ L+   Q + NF+HI  GA++ GKN +PR A+K+ ++ ISDII I+S +TFVRTIYAGNAL TV+  EK+   TVRGTSFE A  E  S   + VS A       ++++++ + K+DRPEL  AK+V+SGGRGLKSG+NFK+LY+LADKLNAAVGASRAAVDAGYV NDLQVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+GLV DLFKAVPEM+  LK
Sbjct:   19 RFQSTLVVAEHNNGTLTPITLNAITAASKLGSDVSCLVAGTECTKVAEELSKVKGLKKILVAQHDAYKGALPEDLTPLILATQKQFNFTHICAGASAFGKNLLPRVAAKLDVAPISDIIEIKSADTFVRTIYAGNALSTVKCNEKIKVFTVRGTSFEAAAKEGGSAASEQVSPAPPA--GISEWLEQSLTKSDRPELTSAKVVVSGGRGLKSGENFKLLYDLADKLNAAVGASRAAVDAGYVPNDLQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGLVADLFKAVPEMTEALK 332          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Cavefish
Match: etfa (electron transfer flavoprotein subunit alpha [Source:NCBI gene;Acc:103023592])

HSP 1 Score: 306.22 bits (783), Expect = 1.603e-102
Identity = 183/316 (57.91%), Postives = 226/316 (71.52%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALLK 1084
            RF S+LV+A+H+ G L PITLN +TAASKLG+DV  LV G  C  VAEE SKVKG+K + +A +  +KG L E ++ L+   Q + NF+HI  GA++ GKN +PR A+K+ ++ ISDII I+S +TFVRTIYAGNAL TV+  EK+   TVRGTSFE A  E  S   + VS A       ++++++ + K+DRPEL  AK+V+SGGRGLKSG+NFK+LY+LADKLNAAVGASRAAVDAGYV NDLQVGQTGKIVAP                       IVAINKDPEAPIFQVAD+GLV DLFKAVPEM+  LK
Sbjct:   19 RFQSTLVVAEHNNGTLTPITLNAITAASKLGSDVSCLVAGTECTKVAEELSKVKGLKKILVAQHDAYKGALPEDLTPLILATQKQFNFTHICAGASAFGKNLLPRVAAKLDVAPISDIIEIKSADTFVRTIYAGNALSTVKCNEKIKVFTVRGTSFEAAAKEGGSAASEQVSPAPPA--GISEWLEQSLTKSDRPELTSAKVVVSGGRGLKSGENFKLLYDLADKLNAAVGASRAAVDAGYVPNDLQVGQTGKIVAP----------------------TIVAINKDPEAPIFQVADYGLVADLFKAVPEMTEALK 310          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Cavefish
Match: etfa (electron transfer flavoprotein subunit alpha [Source:NCBI gene;Acc:103023592])

HSP 1 Score: 300.827 bits (769), Expect = 2.310e-100
Identity = 174/282 (61.70%), Postives = 217/282 (76.95%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIV 982
            RF S+LV+A+H+ G L PITLN +TAASKLG+DV  LV G  C  VAEE SKVKG+K + +A +  +KG L E ++ L+   Q + NF+HI  GA++ GKN +PR A+K+ ++ ISDII I+S +TFVRTIYAGNAL TV+  EK+   TVRGTSFE A  E  S   + VS A       ++++++ + K+DRPEL  AK+V+SGGRGLKSG+NFK+LY+LADKLNAAVGASRAAVDAGYV NDLQVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK +V
Sbjct:   19 RFQSTLVVAEHNNGTLTPITLNAITAASKLGSDVSCLVAGTECTKVAEELSKVKGLKKILVAQHDAYKGALPEDLTPLILATQKQFNFTHICAGASAFGKNLLPRVAAKLDVAPISDIIEIKSADTFVRTIYAGNALSTVKCNEKIKVFTVRGTSFEAAAKEGGSAASEQVSPAPPA--GISEWLEQSLTKSDRPELTSAKVVVSGGRGLKSGENFKLLYDLADKLNAAVGASRAAVDAGYVPNDLQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKCLV 298          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Yeast
Match: AIM45 (Putative ortholog of mammalian ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response; ETF-alpha is an electron transfer flavoprotein complex subunit [Source:SGD;Acc:S000006208])

HSP 1 Score: 202.216 bits (513), Expect = 1.546e-62
Identity = 131/320 (40.94%), Postives = 202/320 (63.12%), Query Frame = 2
Query:  140 FASSLVIADHSK-GKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAE----EFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEP---ATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNF-KILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMS 1072
            +AS+L   + SK G ++  +L+++ AA KL N +  ++ G   +  AE     +S     KLV   D S     L E ++ LL K     ++SH +   +S GK+ +PR  + + +  + ++  I+ P TF+R IYAGN + T++ + +   + +R ++F P    +M++ ++E +T     ++      ++K  + K++RPEL  A+ V++GGR LK  + F K+L  LAD L+AA+GA+RA+VD G   N LQ+GQTGK+VAP LYIA+G+SGA+QHLAGMKDSKVIVAIN DP+APIF VAD+GL  DL+K VPE++
Sbjct:   22 YASTLAFIESSKDGSVSRSSLSLLAAAQKLSNPITAVITGSKAEKTAEALKSSYSCSNLEKLVIFED-SKLDTCLPEQLTPLLVKLLKGGDYSHFVVSNSSVGKSVLPRVGALLDVQPVCEVTVIKDPKTFIRPIYAGNIISTIECQAEKKLLIIRASAFPPIAEGSMDSVTIEKRTDIPPCDLN---VTWVKTILTKSERPELTSAQNVVTGGRALKDKETFEKLLSPLADVLHAAIGATRASVDNGLCDNSLQIGQTGKVVAPNLYIAIGVSGAVQHLAGMKDSKVIVAINNDPDAPIFNVADYGLQGDLYKIVPELT 337          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Nematostella
Match: EDO46072 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR94])

HSP 1 Score: 330.872 bits (847), Expect = 3.222e-112
Identity = 181/320 (56.56%), Postives = 246/320 (76.88%), Query Frame = 2
Query:  113 FAKYCFKIRFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMS 1072
            F ++    R AS+LVIA+H+   L P+TLN +TAA+K+G DV  LV G NC  V E  SKVKG+  + +A N  +KG L E ++ L+   Q + NF+HI  G+++ GK  +PR A+ + ++ +SDI+ I S +TFVRTIYAGNA+ T++S + +  +TVRGT+FE A+ +  +V  +  + +++  N+ + F+K+++  +D PEL  AK+V+SGGRG+KSG+NF++LY++A KLNAAVGASRAAVDAGYV ND+QVGQTGK+VAP LYIAVGISGAIQHLAGMKDSK+IVAINKDPEAPIFQVAD+GLVEDLFKAVPE++
Sbjct:   17 FLRFLCASRNASTLVIAEHNDEHLVPVTLNAITAANKIGGDVAALVAGSNCGPVVEALSKVKGLNKILVAQNDAYKGFLPEVLAPLILATQKQFNFTHITAGSSAFGKGLLPRVAAMLDVAPLSDIMEILSEDTFVRTIYAGNAVTTIKSTDPIKVMTVRGTAFEAASTDGGAVTAED-APSSDTNNELSSFVKQDLHVSDLPELTSAKVVVSGGRGMKSGENFQMLYDMAAKLNAAVGASRAAVDAGYVPNDMQVGQTGKMVAPDLYIAVGISGAIQHLAGMKDSKIIVAINKDPEAPIFQVADYGLVEDLFKAVPEIT 335          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Medaka
Match: etfa (electron transfer flavoprotein subunit alpha [Source:NCBI gene;Acc:101169355])

HSP 1 Score: 350.517 bits (898), Expect = 1.300e-119
Identity = 206/315 (65.40%), Postives = 251/315 (79.68%), Query Frame = 2
Query:  137 RFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALL 1081
            RF S+LVIA+HS  KL PITLN +TAASKLG DV  LV G NC  + EE SKVKGVK V +A +  +KG L E ++ L+ + Q + NF+HI  GA++ GKN +PR A+K+ ++ ISDII I+SP+TFVRTIYAGNAL TV+  E V   TVRGT+FE A++E  S   + V+ AA      ++++++ + K+DRPEL GAK+V+SGGRGLKSGDNFK+LY+LADKLNAAVGASRAAVDAG+V ND+QVGQTGKIVAP+LYIAVGISGAIQHLAGMKDSK IVAINKDPEAPIFQVAD+GLV DLFKAVPEM+  L
Sbjct:   19 RFQSTLVIAEHSNEKLTPITLNAITAASKLGGDVACLVAGTNCAKIVEELSKVKGVKKVLVAQHDAYKGALPEELTPLILETQKQFNFTHICAGASAFGKNLLPRVAAKLDVAPISDIIEIKSPDTFVRTIYAGNALSTVKCNEPVKVFTVRGTAFEAASVEGGSAASEDVAPAALA--GVSEWLEQSLTKSDRPELTGAKVVVSGGRGLKSGDNFKLLYDLADKLNAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGLVADLFKAVPEMTEAL 331          
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Planmine SMEST
Match: SMESG000054248.1 (SMESG000054248.1)

HSP 1 Score: 607.831 bits (1566), Expect = 0.000e+0
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 2
Query:   86 MIKSIVFNGFAKYCFKIRFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFAXXXXXXXXXXXXXXXXPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNXXXXXXXXXXXXXYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALLK 1084
            MIKSIVFNGFAKYCFKIRFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFASKIGISVISDIIGIQSPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNAAVGASRAAVDAGYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALLK
Sbjct:    1 MIKSIVFNGFAKYCFKIRFASSLVIADHSKGKLNPITLNVVTAASKLGNDVHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQSKQNFSHILTGATSNGKNFMPRFASKIGISVISDIIGIQSPNTFVRTIYAGNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTKFIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNAAVGASRAAVDAGYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQVADFGLVEDLFKAVPEMSALLK 333          
The following BLAST results are available for this feature:
BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ETFA6.012e-11759.62electron transfer flavoprotein subunit alpha [Sour... [more]
ETFA2.024e-9860.44electron transfer flavoprotein subunit alpha [Sour... [more]
ETFA5.808e-9751.28electron transfer flavoprotein subunit alpha [Sour... [more]
ETFA3.108e-8664.81electron transfer flavoprotein subunit alpha [Sour... [more]
ETFA3.756e-8367.13electron transfer flavoprotein subunit alpha [Sour... [more]
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BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
F27D4.12.125e-10958.10Probable electron transfer flavoprotein subunit al... [more]
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BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
wal5.815e-11460.32gene:FBgn0010516 transcript:FBtr0088082[more]
wal5.815e-11460.32gene:FBgn0010516 transcript:FBtr0088083[more]
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BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
etfa2.206e-12263.81electron transfer flavoprotein subunit alpha [Sour... [more]
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BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
kif77.615e-11960.76kinesin family member 7 [Source:Xenbase;Acc:XB-GEN... [more]
kif71.207e-11359.68kinesin family member 7 [Source:Xenbase;Acc:XB-GEN... [more]
kif72.382e-11360.46kinesin family member 7 [Source:Xenbase;Acc:XB-GEN... [more]
kif74.826e-11360.46kinesin family member 7 [Source:Xenbase;Acc:XB-GEN... [more]
kif75.559e-11360.46kinesin family member 7 [Source:Xenbase;Acc:XB-GEN... [more]
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BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Etfa6.468e-11358.99electron transferring flavoprotein, alpha polypept... [more]
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BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8HXY0|ETFA_MACFA2.646e-11659.62Electron transfer flavoprotein subunit alpha, mito... [more]
sp|P13804|ETFA_HUMAN2.887e-11659.62Electron transfer flavoprotein subunit alpha, mito... [more]
sp|Q5RC31|ETFA_PONAB1.102e-11559.31Electron transfer flavoprotein subunit alpha, mito... [more]
sp|Q2KJE4|ETFA_BOVIN1.040e-11260.57Electron transfer flavoprotein subunit alpha, mito... [more]
sp|Q99LC5|ETFA_MOUSE4.524e-11258.99Electron transfer flavoprotein subunit alpha, mito... [more]
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BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4W5NNI54.090e-12365.08Electron transfer flavoprotein subunit alpha OS=Hu... [more]
B5X7Q64.713e-12365.08electron transfer flavoprotein subunit alpha, mito... [more]
B5RI575.306e-12365.08Electron-transfer-flavoprotein alpha polypeptide (... [more]
A0A060WFG65.372e-12364.76ETF domain-containing protein OS=Oncorhynchus myki... [more]
A0A1S3IHU02.178e-12264.87electron transfer flavoprotein subunit alpha, mito... [more]
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BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
etfa5.409e-12464.56electron transfer flavoprotein subunit alpha [Sour... [more]
etfa1.603e-10257.91electron transfer flavoprotein subunit alpha [Sour... [more]
etfa2.310e-10061.70electron transfer flavoprotein subunit alpha [Sour... [more]
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BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
AIM451.546e-6240.94Putative ortholog of mammalian ETF-alpha; interact... [more]
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BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO460723.222e-11256.56Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
etfa1.300e-11965.40electron transfer flavoprotein subunit alpha [Sour... [more]
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BLAST of electron transfer flavoprotein subunit alpha, mitochondrial vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000054248.10.000e+0100.00SMESG000054248.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001244 ID=SMED30001244|Name=electron transfer flavoprotein subunit alpha, mitochondrial|organism=Schmidtea mediterranea sexual|type=transcript|length=1139bp
GTTTTAAACTATCAACTTTCATATATTATAAAAATAATTTGAATTATTTA
TAATTTGATTTTCCTATTAGCATTAGGCCATTTCAATGATAAAGTCAATA
GTTTTCAACGGATTTGCAAAATATTGCTTTAAGATAAGATTTGCTAGCTC
TTTGGTAATTGCAGATCACTCGAAAGGGAAATTAAATCCTATTACACTAA
ATGTTGTAACAGCAGCATCTAAGTTAGGCAATGATGTACACGGTTTAGTC
ATTGGAGATAATTGTAAATCAGTAGCTGAAGAATTTTCCAAAGTTAAAGG
AGTAAAGCTAGTATATTTAGCTGACAATTCTATATTTAAAGGTCAATTAG
CTGAAAGTATTTCAGGTTTATTGGAAAAATTTCAATCAAAGCAGAATTTT
TCACACATTTTAACCGGAGCAACATCCAATGGAAAAAATTTTATGCCTCG
ATTTGCTTCGAAAATTGGAATTTCTGTTATTTCCGATATTATCGGGATCC
AATCGCCAAATACTTTCGTTCGCACCATTTACGCTGGAAATGCTCTTCAA
ACTGTTCAAAGCGAGGAAAAGGTTCATTGCATTACAGTTCGTGGAACTTC
TTTTGAACCAGCCACAATGGAAAATAATTCAGTGGAAATTAAAACAGTTT
CAGAAGCGGCTGAAGTAAAAAATGATAAAACCAAATTTATTAAAGAGGAA
ATGGTGAAAAATGATCGACCAGAATTAGCTGGTGCTAAAATTGTTATATC
AGGAGGCAGAGGACTTAAAAGTGGTGATAATTTCAAAATACTTTATGAGT
TGGCGGATAAATTGAATGCAGCAGTTGGGGCTTCTAGAGCAGCAGTGGAT
GCAGGCTATGTAGCTAATGACTTACAAGTTGGCCAAACAGGGAAAATTGT
TGCCCCACAATTATATATTGCCGTAGGCATCAGTGGGGCCATACAGCATC
TAGCAGGTATGAAGGATAGCAAGGTTATTGTTGCCATTAATAAAGATCCA
GAGGCTCCCATATTTCAAGTTGCTGATTTTGGACTGGTGGAAGATCTATT
TAAAGCAGTTCCTGAAATGTCTGCTCTGTTGAAATAAATTATTTAAATTT
GTTTTTAATCAAAAATTATTTCCTATGAAAAAGATCGGA
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protein sequence of SMED30001244-orf-1

>SMED30001244-orf-1 ID=SMED30001244-orf-1|Name=SMED30001244-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=334bp
MIKSIVFNGFAKYCFKIRFASSLVIADHSKGKLNPITLNVVTAASKLGND
VHGLVIGDNCKSVAEEFSKVKGVKLVYLADNSIFKGQLAESISGLLEKFQ
SKQNFSHILTGATSNGKNFMPRFASKIGISVISDIIGIQSPNTFVRTIYA
GNALQTVQSEEKVHCITVRGTSFEPATMENNSVEIKTVSEAAEVKNDKTK
FIKEEMVKNDRPELAGAKIVISGGRGLKSGDNFKILYELADKLNAAVGAS
RAAVDAGYVANDLQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVA
INKDPEAPIFQVADFGLVEDLFKAVPEMSALLK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0005759mitochondrial matrix
Vocabulary: biological process
TermDefinition
GO:0022900electron transport chain
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0003001lateral region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR033947ETF_alpha_N
IPR014730ETF_a/b_N
IPR014731ETF_asu_C
IPR001308ETF_a/FixB
IPR029035DHS-like_NAD/FAD-binding_dom
IPR014729Rossmann-like_a/b/a_fold
IPR018206ETF_asu_C_CS
Vocabulary: molecular function
TermDefinition
GO:0050660flavin adenine dinucleotide binding
GO:0009055electron carrier activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014730Electron transfer flavoprotein, alpha/beta-subunit, N-terminalSMARTSM00893ETF_2coord: 22..198
e-value: 2.6E-36
score: 136.6
IPR014730Electron transfer flavoprotein, alpha/beta-subunit, N-terminalPFAMPF01012ETFcoord: 23..192
e-value: 3.4E-38
score: 131.4
IPR001308Electron transfer flavoprotein alpha subunit/FixBPIRSFPIRSF000089Electra_flavoP_acoord: 18..333
e-value: 1.8E-104
score: 347.4
IPR014731Electron transfer flavoprotein, alpha subunit, C-terminalPFAMPF00766ETF_alphacoord: 213..295
e-value: 5.2E-36
score: 122.5
NoneNo IPR availableGENE3DG3DSA:3.40.50.1220coord: 207..333
e-value: 1.8E-56
score: 191.3
NoneNo IPR availablePANTHERPTHR43153FAMILY NOT NAMEDcoord: 15..332
NoneNo IPR availablePANTHERPTHR43153:SF1ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIALcoord: 15..332
NoneNo IPR availableSUPERFAMILYSSF52402Adenine nucleotide alpha hydrolases-likecoord: 23..208
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 19..206
e-value: 4.4E-51
score: 175.1
IPR018206Electron transfer flavoprotein subunit alpha, conserved sitePROSITEPS00696ETF_ALPHAcoord: 276..302
IPR033947Electron transfer flavoprotein, alpha subunit, N-terminalCDDcd01715ETF_alphacoord: 21..189
e-value: 4.29235E-55
score: 175.437
IPR029035DHS-like NAD/FAD-binding domain superfamilySUPERFAMILYSSF52467DHS-like NAD/FAD-binding domaincoord: 210..331