Death domain-containing protein

Overview
NameDeath domain-containing protein
Smed IDSMED30001115
Length (bp)2131
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Death domain-containing protein (SMED30001115) t-SNE clustered cells

Violin plots show distribution of expression levels for Death domain-containing protein (SMED30001115) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Death domain-containing protein (SMED30001115) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Death domain-containing protein (SMED30001115) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30001115

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30001115h1SMcG0020175 SmedASXL_010811SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30001115h1SMcG0020175 dd_Smed_v4_10509_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30001115h1SMcG0020175 dd_Smed_v4_10509_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30001115h1SMcG0020175 dd_Smed_v4_10509_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30001115h1SMcG0020175 dd_Smed_v4_10509_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30001115h1SMcG0020175 dd_Smed_v4_10509_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
dorsal region of the epidermisSMED30001115h1SMcG0020175 dd_Smed_v4_10509_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30001115h1SMcG0020175 dd_Smed_v4_10509_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Death domain-containing protein vs. TrEMBL
Match: A0A1I8GEB9 (Death domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027336g1 PE=4 SV=1)

HSP 1 Score: 266.159 bits (679), Expect = 5.074e-76
Identity = 170/597 (28.48%), Postives = 298/597 (49.92%), Query Frame = 2 
Query:  188 MPVFTTLEFFRSKQTIDSFETMYPKSMSGLREAIHGSLDTISNMLTELIHQYLNGYPDQESFIRDKAISLHLLLIETGAEGVYRKMDFELICDRAGA----SSALNEHINKIKVISKDISEYNPNAFTPIVQLFDNILNCMEKYFLSFYTGKIHLCPSRFIETPFNYENKRLEIIQGIKKKLRRIFNLSNQFNTNGIKITDMGDKSNHIARRAKSLEMPCFLLFPESCDSIEEAKDLLLQWIEMDKEYPKMIKIDINCMNEEKNDLEQKRRDLYDECVKSNHLTDQQKRDYEEMFSLKTAMLTKLQASENE--------------SHETLVKIKHNEFIIKKSNLIIKELERKIESLGSSDKFEKDVNDHTFTIHKVIKIMKENKIKLKRIENKINWYKRYLKNLTLK----------KIELDKAVASNSKIGTQLEELDGKITTINDYLNQLHSILNLKSSNSTLKKIFHNLDLKTKL--SFLPEKNKNKQIVVKDE-----LINAIQ----ITSHLIEKDWIKLYENLPFHPPRGTSEIENDIEKCIKLHVRGSREDLALTILQTWRRFHIRANLLSLVDSLQKINRFDIIKKLD 1861
            MP  TTL F+R  QT+DSF    PKS+  LR A    +D +  +L E+   YL+ +  QE FIR+   +   L++  G   + +  D E + +   +    S    E + K++ + + + ++ P  FTP  +    ++ C+ KY LSFY G     P ++++TPF YE KRLE+   ++K +  +      F + G+ +         +ARR    E+   LL PE+C+ + EA  LL  WIE+DKEY + +  D+  +  E++   +K ++ Y + V+  H               + A+L ++ A E E               H  LV++K  E+ + ++  ++ +++R  E+  S ++ ++   + T         +K  K    +I  K+   +  +K+   +           +EL   +  +S+I   L+E   K+     Y   L  I  LK S S++KKIFHNL +  +L  S + E++ +  +++  E     L N IQ    + +  IE+DW +LY  LPF+PPRG  +++ D+++  K  VRG+R++LA  +L TWRR H RAN+ +L ++L +I R D+ +++D
Sbjct:    1 MPAVTTLAFYREPQTVDSFLRERPKSLLALRAACRQEIDAVLVVLREIELDYLHPHETQEDFIRENLFTFKRLMMAGGIREIRKTFDIENMMESLNSLGTESIKYTESMQKVRQLQQHVGDFLPEKFTPFNERLTKVMQCLRKYCLSFYRGTTQRSPDKYLDTPFKYEQKRLELTTRMQKIIMGLTEKFFVFGSVGLGLQGFSYNVQQLARRVGCGEISLLLLLPEACEQVREACQLLNNWIELDKEYVENLGNDLVGIELEQSSASRKYQEEYTKMVQLKH--------------RQAAILKRIDAYEKELPDFQQKKKHVGRRHHLALVEMKKCEYHLHRAKTVLGDIKRHPETFFSEEEMQRAKLESTLE-------LKRQKTIFPKISKKVEITESIVKHCAQRDSEHEADKRAAMELQFQIDKSSRIVAALQE---KLERSTRYEKVLRHIFTLKQSPSSMKKIFHNLQMDDRLQNSLIKERSVSPTLMLAPEIEEYTLGNPIQMACRVVAKAIEQDWFRLYFQLPFYPPRGQVDLDADVDRINKRLVRGNRQELATEMLATWRRLHTRANVEALKEALGRIGRQDLSQRVD 573
[Close]
BLAST of Death domain-containing protein vs. TrEMBL
Match: A0A1I8J2G3 (Death domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027336g2 PE=4 SV=1)

HSP 1 Score: 266.159 bits (679), Expect = 5.628e-76
Identity = 170/597 (28.48%), Postives = 298/597 (49.92%), Query Frame = 2 
Query:  188 MPVFTTLEFFRSKQTIDSFETMYPKSMSGLREAIHGSLDTISNMLTELIHQYLNGYPDQESFIRDKAISLHLLLIETGAEGVYRKMDFELICDRAGA----SSALNEHINKIKVISKDISEYNPNAFTPIVQLFDNILNCMEKYFLSFYTGKIHLCPSRFIETPFNYENKRLEIIQGIKKKLRRIFNLSNQFNTNGIKITDMGDKSNHIARRAKSLEMPCFLLFPESCDSIEEAKDLLLQWIEMDKEYPKMIKIDINCMNEEKNDLEQKRRDLYDECVKSNHLTDQQKRDYEEMFSLKTAMLTKLQASENE--------------SHETLVKIKHNEFIIKKSNLIIKELERKIESLGSSDKFEKDVNDHTFTIHKVIKIMKENKIKLKRIENKINWYKRYLKNLTLK----------KIELDKAVASNSKIGTQLEELDGKITTINDYLNQLHSILNLKSSNSTLKKIFHNLDLKTKL--SFLPEKNKNKQIVVKDE-----LINAIQ----ITSHLIEKDWIKLYENLPFHPPRGTSEIENDIEKCIKLHVRGSREDLALTILQTWRRFHIRANLLSLVDSLQKINRFDIIKKLD 1861
            MP  TTL F+R  QT+DSF    PKS+  LR A    +D +  +L E+   YL+ +  QE FIR+   +   L++  G   + +  D E + +   +    S    E + K++ + K + ++ P  FTP  +    ++ C+ KY LSFY G     P ++++TPF YE KRLE+   ++K +  +      F + G+ +         +ARR    E+   LL PE+C+ + EA  LL  WIE+DKEY + +  D+  +  E++   +K ++ Y + V+  H               + A+L ++ A E E               H  LV++K  E+ + ++  ++ +++R  E+  + ++ ++   + T         +K  K    +I  K+   +  +K+   +           +EL   +  +S+I   L+E   K+     Y   L  I  LK S S++KKIFHNL +  +L  S + E++ +  +++  E     L N IQ    + +  IE+DW +LY  LPF+PPRG  +++ D+++  K  VRG+R++LA  +L TWRR H RAN+ +L ++L +I R D+ +++D
Sbjct:    1 MPAVTTLAFYREPQTVDSFLRERPKSLLALRAACRQEIDAVLVVLREIELDYLHPHETQEDFIRENLFTFKRLMMAGGIREIRKTFDIENMMESLNSLGTESIKYTESMQKVRQLQKHVGDFLPEKFTPFNERLTKVMQCLRKYCLSFYRGTTQRSPDKYLDTPFKYEQKRLELTTRMQKIIMGLTEKFFVFGSVGLGLQGFSYNVQQLARRVGCGEISLLLLLPEACEQVREACQLLNNWIELDKEYVENLGNDLVGIELEQSSASRKYQEEYTKMVQLKH--------------RQAAILKRIDAYEKELPDFQQKKKHVGRRHHLALVEMKKCEYHLHRAKTVLGDIKRHPETFFTEEEMQRAKLESTLE-------LKRQKTIFPKISKKVEITESIVKHCAQRDSEHEADKRAAMELQFQIDKSSRIVAALQE---KLERSTRYEKVLRHIFTLKQSPSSMKKIFHNLQMDDRLQNSLIKERSVSPTLMLAPEIEEYTLGNPIQMACRVVAKAIEQDWFRLYFQLPFYPPRGQVDLDADVDRINKRLVRGNRQELATEMLATWRRLHTRANVEALKEALGRIGRQDLSQRVD 573
[Close]
BLAST of Death domain-containing protein vs. TrEMBL
Match: A0A1I8J1A2 (Death domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017962g1 PE=4 SV=1)

HSP 1 Score: 265.774 bits (678), Expect = 7.144e-76
Identity = 170/597 (28.48%), Postives = 298/597 (49.92%), Query Frame = 2 
Query:  188 MPVFTTLEFFRSKQTIDSFETMYPKSMSGLREAIHGSLDTISNMLTELIHQYLNGYPDQESFIRDKAISLHLLLIETGAEGVYRKMDFELICDRAGA----SSALNEHINKIKVISKDISEYNPNAFTPIVQLFDNILNCMEKYFLSFYTGKIHLCPSRFIETPFNYENKRLEIIQGIKKKLRRIFNLSNQFNTNGIKITDMGDKSNHIARRAKSLEMPCFLLFPESCDSIEEAKDLLLQWIEMDKEYPKMIKIDINCMNEEKNDLEQKRRDLYDECVKSNHLTDQQKRDYEEMFSLKTAMLTKLQASENE--------------SHETLVKIKHNEFIIKKSNLIIKELERKIESLGSSDKFEKDVNDHTFTIHKVIKIMKENKIKLKRIENKINWYKRYLKNLTLK----------KIELDKAVASNSKIGTQLEELDGKITTINDYLNQLHSILNLKSSNSTLKKIFHNLDLKTKL--SFLPEKNKNKQIVVKDE-----LINAIQ----ITSHLIEKDWIKLYENLPFHPPRGTSEIENDIEKCIKLHVRGSREDLALTILQTWRRFHIRANLLSLVDSLQKINRFDIIKKLD 1861
            MP  TTL F+R  QT+DSF    PKS+  LR A    +D +  +L E+   YL+ +  QE FIR+   +   L++  G   + +  D E + +   +    S    E + K++ + + + ++ P  FTP  +    ++ C+ KY LSFY G     P ++++TPF YE KRLE+   ++K +  +      F + G+ +         +ARR    E+   LL PE+C+ + EA  LL  WIE+DKEY + +  D+  +  E++   +K ++ Y + V+  H               + A+L ++ A E E               H  LV++K  E+ + ++  ++ +++R  E+  + ++ ++   + T         +K  K    +I  K+   +  +K+   +           +EL   +  +S+I   L+E   K+     Y   L  I  LK S S++KKIFHNL L  +L  S + E++ +  +++  E     L N IQ    + +  IE+DW +LY  LPF+PPRG  +++ D+++  K  VRG+R++LA  +L TWRR H RAN+ +L ++L +I R D+ +++D
Sbjct:    1 MPAVTTLAFYREPQTVDSFLRERPKSLLALRAACRQEIDAVLVVLREIELDYLHPHETQEDFIRENLFTFKRLMMAGGIREIRKTFDIENMMESLNSLGTESIKYTESMQKVRQLQQHVGDFLPEKFTPFNERLTKVMQCLRKYCLSFYRGTTQRSPDKYLDTPFKYEQKRLELTTRMQKIIMGLTEKFFVFGSVGLGLQGFSYNVQQLARRVGCGEISLLLLLPEACEQVREACQLLNNWIELDKEYVENLGNDLVGIELEQSSASRKYQEEYTKMVQLKH--------------RQAAILKRIDAYEKELPDFQQKKKHVGRRHHLALVEMKKCEYHLHRAKTVLGDIKRHPETFFTEEEMQRAKLESTLE-------LKRQKTIFPKISKKVEITESIVKHCAQRDSEHEADKRAAMELQFQIDKSSRIVAALQE---KLERSTRYEKVLRHIFTLKQSPSSMKKIFHNLQLDDRLQNSLIKERSVSPTLMLAPEIEEYTLGNPIQMACRVVAKAIEQDWFRLYFQLPFYPPRGQVDLDADVDRINKRLVRGNRQELATEMLATWRRLHTRANVEALKEALGRIGRQDLSQRVD 573
[Close]
BLAST of Death domain-containing protein vs. TrEMBL
Match: A0A1S3HCR9 (uncharacterized protein LOC106154088 OS=Lingula unguis OX=7574 GN=LOC106154088 PE=4 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 1.059e-45
Identity = 162/574 (28.22%), Postives = 269/574 (46.86%), Query Frame = 2 
Query:  191 PVFTTLEFFRSKQTIDSFETMYPKSMSGLREAIHGSLDTISNMLTELIHQYLNG----YPDQESFIRDKAISLHLLLIETGAEGVYRKMDFELICDRA-GASSALNEH----INKIKVISKDISEYNPNAFTPIVQLFDNILNCMEKYFLSFYTGKIHLCPSRFIETPFNYENKRLEIIQGIKKKLRRIFNLSNQFNTNGIKITDMGDKSNHIARRAKSLEMPCFLLFPESCDSIEEAKDLLLQWIEMDKEYPKMIKIDINCMNEEKNDLEQKRRDLYDECVKSNHLTDQQKRDYEEMFSLKTAMLTKLQASENESHETLVKIKHNEFIIKKSNLIIKELERK--------IESLGSSDKFEKDVNDHTFTIHKVIKIMKENKIKLKRIENKINWYKRYLKNLTLKKIELDKAVASNSKIGTQLEELDGKITTINDYLNQLHSILNLKSSNSTLKKIFHNLDLKTKLSFLPEKNKNKQIVVKDELINAIQITSHLIEKDWIKLYENLPFHPPRGTSEIENDIEKCIKLHVRGSREDLALTILQTWRRFHIRANLLSLVDSLQKINRFDIIKKLD 1861
            P   TL+F R  QT++  +   PKS+  +RE +  +   +   L+ LI   L G    Y D   FIR     +  L+       +Y K   E I  +      A+ E      + +K     I EY P AFT I    + IL  ++KY  SF   +       F++TP +YE KR E+ + I+  L  I ++  ++  +G+ I   G  +  I R  +   +P  L+FP++C ++  A   + QW++ D+EYP  IK DI  M ++   +E K R+  +  ++S+ L + + R  ++  S+  + L KL A E   ++   K++     I K  L I  LE++        I+S    +   + ++D     H +       + K+  +E K    +    N +  +    KA   ++ +  +L        T+N+ L +L  I   K+S+ T KKIF+ L L  K +      KN      D L  A++I +H IEKDW+KLY  LPFHP RGT  +E D++   +   R + ++L+   L  WRR H RA L  L  +L ++ R D+++ L+
Sbjct:    3 PATATLQFSRKSQTLEELDKERPKSLEAVREILREATTDVETKLS-LIEVSLIGRGVTYAD---FIRPYVAPIVSLMKSLEIYEIYNKKQIEEIIHQGIETGEAVKESDLPGYHMLKECYHKIKEYLPGAFTAIFLEIEKILENLDKYCRSFSKAE----EREFMDTPSHYEMKRQELWKLIRVNLDDIKDMIGRYQEDGVDIKMFGIHARDIGRGCECERLPFLLMFPQTCVNVRNACKGIRQWLKADEEYPSYIKDDIIDMEKK---IEHKTREERESQMRSSQL-EYKMRHVQQECSVLESELKKLIAREMAFNQDARKLERE---INKLELEIGTLEQEKVMSSKGLIKSSQPPEALSRKIHDLKEKHHGLATKHSHVQAKIMLVEKKREMLRESNANASALRSASRKARTDHTNVEHELH-------TMNERLEELKQIYLYKTSHETPKKIFYGLPLAPKTTVKTTGKKNH-----DPLNRAVKIVAHYIEKDWMKLYRKLPFHPVRGTEMLEQDLDDIFQKSARNTVKELSHEALAKWRRLHTRATLEELKGALVELKRKDVLEHLE 549
[Close]
BLAST of Death domain-containing protein vs. TrEMBL
Match: A0A1S3I4A2 (uncharacterized protein LOC106160203 OS=Lingula unguis OX=7574 GN=LOC106160203 PE=4 SV=2)

HSP 1 Score: 182.185 bits (461), Expect = 1.811e-45
Identity = 159/574 (27.70%), Postives = 272/574 (47.39%), Query Frame = 2 
Query:  191 PVFTTLEFFRSKQTIDSFETMYPKSMSGLREAIHGSLDTISNMLTELIHQYLNG----YPDQESFIRDKAISLHLLLIETGAEGVYRKMDFELICDRA-GASSALNEH----INKIKVISKDISEYNPNAFTPIVQLFDNILNCMEKYFLSFYTGKIHLCPSRFIETPFNYENKRLEIIQGIKKKLRRIFNLSNQFNTNGIKITDMGDKSNHIARRAKSLEMPCFLLFPESCDSIEEAKDLLLQWIEMDKEYPKMIKIDINCMNEEKNDLEQKRRDLYDECVKSNHLTDQQKRDYEEMFSLKTAMLTKLQASENESHETLVKIKHNEFIIKKSNLIIKELERK--------IESLGSSDKFEKDVNDHTFTIHKVIKIMKENKIKLKRIENKINWYKRYLKNLTLKKIELDKAVASNSKIGTQLEELDGKITTINDYLNQLHSILNLKSSNSTLKKIFHNLDLKTKLSFLPEKNKNKQIVVKDELINAIQITSHLIEKDWIKLYENLPFHPPRGTSEIENDIEKCIKLHVRGSREDLALTILQTWRRFHIRANLLSLVDSLQKINRFDIIKKLD 1861
            P   TL+F R  QT++  +   PKS+  +RE +  +   +   L+ LI   L G    Y D   FIR     +  L+       +Y K   E I  +      A+ E      + +K     I EY P AFT I    + IL  ++KY  SF   +       +++TP +YE KR E+ + I+  L  + ++  ++  +G+ I   G  +  I R  +   +P  L+FP++C ++  A   + QW++ D+EYP  IK DI  M ++   +E K R+  +  ++S+ L + + R  ++  S+  + L KL A E   ++   K++     I K  L I  LE++        I+S    +   + ++D     H +       + K+  +E K    +    N +  +       +++ K  T+   ++ ++ T+N+ L +L  I   K+S+ T KKIF+ L L  K +      KN      D L  A++I +H IEKDW+KLY  LPFHP RGT  +E D++   +   R + ++L+   L  WRR H RA L  L  +L ++ R D+++ L+
Sbjct:    3 PATATLQFSRKSQTLEELDKERPKSLEAVREILREATTDVETKLS-LIEVSLIGRGVTYAD---FIRPYVAPIVSLMKSLEIYEIYNKKQIEEIIHQGIETGEAVKESDLPGYHMLKECYHKIKEYLPGAFTAIFLEIEKILENLDKYCRSFSKAE----EREYMDTPSHYEMKRQELWKLIRVNLDDVKDMIGRYQEDGVDIKMFGIHARDIGRGCECERLPFLLMFPQTCVNVRNACKGIRQWLKADEEYPSYIKDDIIDMEKK---IEHKTREERESQMRSSQL-EYKMRHVQQECSVLESELKKLIAREMAFNQDARKLERE---INKLELEIGTLEQEKVMSSKGLIKSSHPPEALSRKIHDLKEKHHGLATKHSHVQAKIMLVEKKREMLRESNANASALR-------SASRKARTEHTNVEHELHTMNERLEELKQIYLYKTSHETPKKIFYGLPLAPKTTVKTTGKKNH-----DPLNRAVKIVAHYIEKDWMKLYRKLPFHPVRGTEMLEQDLDDIFQKSARNTVKELSHEALAKWRRLHTRATLEELKGALVELKRKDVLEHLE 549
[Close]
BLAST of Death domain-containing protein vs. Planmine SMEST
Match: SMESG000034716.1 (SMESG000034716.1)

HSP 1 Score: 1224.92 bits (3168), Expect = 0.000e+0
Identity = 600/601 (99.83%), Postives = 600/601 (99.83%), Query Frame = 2 
Query:  188 MPVFTTLEFFRSKQTIDSFETMYPKSMSGLREAIHGSLDTISNMLTELIHQYLNGYPDQESFIRDKAISLHLLLIETGAEGVYRKMDFELICDRAGASSALNEHINKIKVISKDISEYNPNAFTPIVQLFDNILNCMEKYFLSFYTGKIHLCPSRFIETPFNYENKRLEIIQGIKKKLRRIFNLSNQFNTNGIKITDMGDKSNHIARRAKSLEMPCFLLFPESCDSIEEAKDLLLQWIEMDKEYPKMIKIDINCMNEEKNDLEQKRRDLYDECVKSNHLTDQQKRDYEEMFSLKTAMLTKLQASENESHETLVKIKHNEFIIKKSNLIIKELERKIESLGSSDKFEKDVNDHTFTIHKVIKIMKENKIKLKRIENKINWYKRYLKNLTLKKIELDKAVASNSKIGTQLEELDGKITTINDYLNQLHSILNLKSSNSTLKKIFHNLDLKTKLSFLPEKNKNKQIVVKDELINAIQITSHLIEKDWIKLYENLPFHPPRGTSEIENDIEKCIKLHVRGSREDLALTILQTWRRFHIRANLLSLVDSLQKINRFDIIKKLDFEVTLKQIPSMHRFDDEKLPPMSATTRFLIKHYPNLQKTRSQT 1990
            MPVFTTLEFFRSKQTIDSFETMYPKSMSGLREAIHGSLDTISNMLTELIHQYLNGYPDQESFIRDKAISLHLLLIETGAEGVYRKMDFELICDRAGASSALNEHINKIKVISKDISEYNPNAFTPIVQLFDNILNCMEKYFLSFYTGKIHLCPSRFIETPFNYENKRLEIIQGIKKKLRRIFNLSNQFNTNGIKITDMGDKSNHIARRAKSLEMPCFLLFPESCDSIEEAKDLLLQWIEMDKEYPKMIKIDINCMNEEKNDLEQKRRDLYDECVKSNHLTDQQKRDYEEMFSLKTAMLTKLQASENESHETLVKIKHNEFIIKKSNLIIKELERKIESLGSSDKFEKDVNDHTFTIHKV KIMKENKIKLKRIENKINWYKRYLKNLTLKKIELDKAVASNSKIGTQLEELDGKITTINDYLNQLHSILNLKSSNSTLKKIFHNLDLKTKLSFLPEKNKNKQIVVKDELINAIQITSHLIEKDWIKLYENLPFHPPRGTSEIENDIEKCIKLHVRGSREDLALTILQTWRRFHIRANLLSLVDSLQKINRFDIIKKLDFEVTLKQIPSMHRFDDEKLPPMSATTRFLIKHYPNLQKTRSQT
Sbjct:    1 MPVFTTLEFFRSKQTIDSFETMYPKSMSGLREAIHGSLDTISNMLTELIHQYLNGYPDQESFIRDKAISLHLLLIETGAEGVYRKMDFELICDRAGASSALNEHINKIKVISKDISEYNPNAFTPIVQLFDNILNCMEKYFLSFYTGKIHLCPSRFIETPFNYENKRLEIIQGIKKKLRRIFNLSNQFNTNGIKITDMGDKSNHIARRAKSLEMPCFLLFPESCDSIEEAKDLLLQWIEMDKEYPKMIKIDINCMNEEKNDLEQKRRDLYDECVKSNHLTDQQKRDYEEMFSLKTAMLTKLQASENESHETLVKIKHNEFIIKKSNLIIKELERKIESLGSSDKFEKDVNDHTFTIHKVTKIMKENKIKLKRIENKINWYKRYLKNLTLKKIELDKAVASNSKIGTQLEELDGKITTINDYLNQLHSILNLKSSNSTLKKIFHNLDLKTKLSFLPEKNKNKQIVVKDELINAIQITSHLIEKDWIKLYENLPFHPPRGTSEIENDIEKCIKLHVRGSREDLALTILQTWRRFHIRANLLSLVDSLQKINRFDIIKKLDFEVTLKQIPSMHRFDDEKLPPMSATTRFLIKHYPNLQKTRSQT 601
The following BLAST results are available for this feature:
BLAST of Death domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Death domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Death domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Death domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Death domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Death domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Death domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Death domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8GEB95.074e-7628.48Death domain-containing protein OS=Macrostomum lig... [more]
A0A1I8J2G35.628e-7628.48Death domain-containing protein OS=Macrostomum lig... [more]
A0A1I8J1A27.144e-7628.48Death domain-containing protein OS=Macrostomum lig... [more]
A0A1S3HCR91.059e-4528.22uncharacterized protein LOC106154088 OS=Lingula un... [more]
A0A1S3I4A21.811e-4527.70uncharacterized protein LOC106160203 OS=Lingula un... [more]
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BLAST of Death domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Death domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Death domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Death domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Death domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Death domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000034716.10.000e+099.83SMESG000034716.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001115 ID=SMED30001115|Name=Death domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2131bp
ATAACGGCTACGCTGTAAAAATAGATCATTTAAGACGACAATATTATCTG
CACTTCAAACGGTGATGCTATAGTTACTGTCAGAAAGAACAATTATGAAC
AATTAATCAGAAAGAGTTACTATTTGCAGAGATTGCAGGTTGACAGAGTA
TTTCTCACTAATATTCATTGTAAAGATTTCGCCAAAAATGCCAGTTTTCA
CGACTCTGGAGTTTTTCCGATCAAAACAGACAATCGACAGTTTTGAAACA
ATGTATCCTAAATCAATGTCGGGATTGCGAGAAGCGATTCACGGTTCACT
TGACACAATCAGCAACATGCTCACTGAACTCATCCATCAATACCTCAATG
GCTACCCGGATCAGGAATCTTTCATTCGTGACAAGGCCATCAGTTTGCAT
TTGCTCCTGATTGAAACCGGAGCGGAAGGAGTTTATCGGAAAATGGATTT
CGAGTTGATCTGCGATCGAGCTGGAGCCTCAAGTGCATTGAATGAGCACA
TCAATAAAATCAAGGTGATTAGTAAAGATATTTCAGAATACAATCCAAAT
GCCTTTACACCGATTGTCCAGCTGTTTGACAATATTCTCAATTGCATGGA
AAAATATTTTCTTAGTTTTTATACGGGTAAAATTCATCTCTGCCCTAGCA
GGTTCATCGAAACCCCGTTCAACTACGAGAATAAACGCCTGGAAATTATA
CAAGGCATAAAGAAAAAACTTCGAAGAATATTTAATTTATCAAATCAATT
TAACACAAACGGCATAAAAATAACTGACATGGGGGACAAAAGCAATCACA
TTGCCAGACGGGCAAAATCTTTGGAAATGCCATGTTTTCTATTATTCCCG
GAAAGTTGCGATTCTATAGAAGAGGCGAAAGATTTATTATTGCAATGGAT
CGAAATGGACAAAGAATACCCAAAAATGATTAAAATTGATATTAATTGCA
TGAATGAGGAAAAGAACGATCTAGAACAGAAACGAAGAGATCTGTACGAT
GAATGCGTTAAGTCCAACCATTTGACGGACCAGCAGAAGCGTGATTACGA
GGAAATGTTCTCATTAAAAACTGCAATGTTGACCAAATTGCAAGCTAGTG
AAAATGAATCTCATGAAACGTTGGTCAAAATTAAGCATAATGAATTCATA
ATTAAAAAGTCCAATCTCATCATCAAAGAATTAGAAAGAAAAATCGAAAG
TCTTGGAAGCTCCGATAAATTTGAGAAGGATGTCAATGATCATACTTTTA
CAATTCACAAAGTAATCAAAATAATGAAAGAAAATAAGATAAAGCTTAAA
AGAATTGAAAATAAGATAAATTGGTACAAAAGATATTTAAAAAACCTAAC
ATTGAAAAAAATAGAACTGGATAAAGCGGTGGCCAGCAATTCAAAAATTG
GAACCCAGTTGGAAGAACTTGATGGGAAAATAACAACGATAAATGATTAT
CTCAATCAATTGCACTCGATTTTGAATTTGAAATCGTCAAATTCTACTTT
AAAAAAGATTTTCCATAACTTGGATTTGAAAACTAAACTGAGTTTCTTAC
CGGAAAAGAATAAAAACAAGCAAATAGTTGTCAAAGATGAATTAATAAAT
GCAATTCAAATAACGAGCCATCTAATTGAGAAAGATTGGATAAAGCTTTA
TGAAAATCTTCCATTTCATCCTCCAAGAGGCACTAGTGAAATTGAAAACG
ATATTGAAAAATGTATAAAATTACATGTTCGAGGATCTCGGGAAGATTTG
GCTCTGACAATTCTTCAAACTTGGAGAAGATTTCACATCCGAGCAAATTT
ATTGTCACTGGTAGACTCTCTGCAAAAAATAAATAGATTTGATATAATCA
AAAAATTGGACTTCGAGGTGACATTAAAACAAATTCCTTCAATGCACCGT
TTCGATGATGAAAAGCTGCCTCCAATGTCTGCAACTACAAGATTTTTAAT
CAAGCATTATCCGAATTTACAAAAGACTAGATCTCAAACAATACAATAAA
AATAACAACAAAAAATATTAATGAAAATTTTGCTGGATTTCTACAGAATA
TCCTTTTATTACTGTTGTTGGTTTGTACTCTGACCTCTTTCGTGTTTTTG
GGTATCATTAATCCAATACATAAAGAAAAAT
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protein sequence of SMED30001115-orf-1

>SMED30001115-orf-1 ID=SMED30001115-orf-1|Name=SMED30001115-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=604bp
MPVFTTLEFFRSKQTIDSFETMYPKSMSGLREAIHGSLDTISNMLTELIH
QYLNGYPDQESFIRDKAISLHLLLIETGAEGVYRKMDFELICDRAGASSA
LNEHINKIKVISKDISEYNPNAFTPIVQLFDNILNCMEKYFLSFYTGKIH
LCPSRFIETPFNYENKRLEIIQGIKKKLRRIFNLSNQFNTNGIKITDMGD
KSNHIARRAKSLEMPCFLLFPESCDSIEEAKDLLLQWIEMDKEYPKMIKI
DINCMNEEKNDLEQKRRDLYDECVKSNHLTDQQKRDYEEMFSLKTAMLTK
LQASENESHETLVKIKHNEFIIKKSNLIIKELERKIESLGSSDKFEKDVN
DHTFTIHKVIKIMKENKIKLKRIENKINWYKRYLKNLTLKKIELDKAVAS
NSKIGTQLEELDGKITTINDYLNQLHSILNLKSSNSTLKKIFHNLDLKTK
LSFLPEKNKNKQIVVKDELINAIQITSHLIEKDWIKLYENLPFHPPRGTS
EIENDIEKCIKLHVRGSREDLALTILQTWRRFHIRANLLSLVDSLQKINR
FDIIKKLDFEVTLKQIPSMHRFDDEKLPPMSATTRFLIKHYPNLQKTRSQ
TIQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000054dorsal region of the epidermis
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR000488Death_domain
IPR011029DEATH-like_dom_sf
Vocabulary: biological process
TermDefinition
GO:0007165signal transduction
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 252..272
NoneNo IPR availableGENE3DG3DSA:1.10.533.10coord: 458..562
e-value: 6.6E-7
score: 31.3
IPR000488Death domainPROSITEPS50017DEATH_DOMAINcoord: 525..561
score: 9.686
IPR011029Death-like domain superfamilySUPERFAMILYSSF47986DEATH domaincoord: 469..557