SMED30001042

Overview
NameSMED30001042
Smed IDSMED30001042
Length (bp)1596
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30001042 (SMED30001042) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30001042 (SMED30001042) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30001042 (SMED30001042) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30001042 (SMED30001042) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Homology
[Close]
BLAST of SMED30001042 vs. TrEMBL
Match: Q7YXU7 (ORF2 (Fragment) OS=Girardia tigrina OX=6162 PE=4 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.529e-16
Identity = 81/266 (30.45%), Postives = 130/266 (48.87%), Query Frame = 3 
Query:  117 LFITSEKRGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDL-ISSHLSKKSNLIVVVD--HVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLLKDCALSNISHSARRW 905
            L I S+++G      D++K  + ++   L  ++L + +K   S  + SA      +NK I  G +    W+FIHRAR   L    K  +  N  LCR C  + ETMSH + +C +  TL +ERH D L++ +   LS K    V+    +V  L P+        IT     + +LV++KCP DT  +F L       KY+ +   ++D  P  +V L T I+G+LGS+ P+  + L  +G  +  +  +     L NI+ S  +W
Sbjct:  840 LLIDSKEKG------DVKKIHASLMGF-LRLAHLIRLQKHGWSKLLFSATTHHEILNKRILNGHVPYKIWYFIHRARLGLLP--TKLFSVSN--LCRKCGGKKETMSHALVNCPMMQTLINERH-DALEISLVQILSSKFQGTVIRQKTYVNELRPD--------ITMESDTQYYLVEVKCPFDTKMSFELRTQQTTDKYNIIIEILEDVHPGKEVRLVTFIVGTLGSWGPQNSDFLRDLGFSKDEIDQVKTRLMLQNINSSCEQW 1085
[Close]
BLAST of SMED30001042 vs. TrEMBL
Match: K7JS43 (C2H2-type domain-containing protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.580e-16
Identity = 59/225 (26.22%), Postives = 112/225 (49.78%), Query Frame = 3 
Query:  183 KVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFA-WHFIHRARTNTLSINA-KPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKKSNLIVVVDHVCSLV-PESKERVDLMITDMVRKKIFLVDMKCPCDT-INNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTH 845
            + L+    +S L+K ++G     +    + S + N  +  G  + F  W FIHRAR + L +NA K   +G  + CR C ++ ET+ H++  C  H     +RHN+  D +    S+    I V   V  +  P++  R D+++ D V +++ +VD+  P +  +  F  V    + KY  L  Q+ D+   + V ++  ++G+LG++ P+   +L  + + R +
Sbjct:  109 RCLNRHYAESLLAKKDQGK----VFEVTRRSGQSNHFLRSGSFTRFCDWRFIHRARLDVLPLNAAKRWQRGMDKRCRRCGSDLETLPHVLSHCGPHSAARQKRHNNIQDRLVKAASRCPGTISVNQTVVGVRGPDAALRPDIVVRDDVNRRVTIVDVAVPFENRLEAFDGVREAKIAKYTPLARQLTDS--GYTVTVEAFVVGALGAWDPRNERVLSLLSISRYY 327
[Close]
BLAST of SMED30001042 vs. TrEMBL
Match: K7JWY8 (C2H2-type domain-containing protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.647e-16
Identity = 59/225 (26.22%), Postives = 112/225 (49.78%), Query Frame = 3 
Query:  183 KVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFA-WHFIHRARTNTLSINA-KPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKKSNLIVVVDHVCSLV-PESKERVDLMITDMVRKKIFLVDMKCPCDT-INNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTH 845
            + L+    +S L+K ++G     +    + S + N  +  G  + F  W FIHRAR + L +NA K   +G  + CR C ++ ET+ H++  C  H     +RHN+  D +    S+    I V   V  +  P++  R D+++ D V +++ +VD+  P +  +  F  V    + KY  L  Q+ D+   + V ++  ++G+LG++ P+   +L  + + R +
Sbjct:  109 RCLNRHYAESLLAKKDQGK----VFEVTRRSGQSNHFLRSGSFTRFCDWRFIHRARLDVLPLNAAKRWQRGMDKRCRRCGSDLETLPHVLSHCGPHSAARQKRHNNIQDRLVKAASRCPGTISVNQTVVGVRGPDAALRPDIVVRDDVNRRVTIVDVAVPFENRLEAFDGVREAKIAKYTPLARQLTDS--GYTVTVEAFVVGALGAWDPRNERVLSLLSISRYY 327
[Close]
BLAST of SMED30001042 vs. TrEMBL
Match: K7JS44 (C2H2-type domain-containing protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.716e-16
Identity = 59/225 (26.22%), Postives = 112/225 (49.78%), Query Frame = 3 
Query:  183 KVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFA-WHFIHRARTNTLSINA-KPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKKSNLIVVVDHVCSLV-PESKERVDLMITDMVRKKIFLVDMKCPCDT-INNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTH 845
            + L+    +S L+K ++G     +    + S + N  +  G  + F  W FIHRAR + L +NA K   +G  + CR C ++ ET+ H++  C  H     +RHN+  D +    S+    I V   V  +  P++  R D+++ D V +++ +VD+  P +  +  F  V    + KY  L  Q+ D+   + V ++  ++G+LG++ P+   +L  + + R +
Sbjct:  109 RCLNRHYAESLLAKKDQGK----VFEVTRRSGQSNHFLRSGSFTRFCDWRFIHRARLDVLPLNAAKRWQRGMDKRCRRCGSDLETLPHVLSHCGPHSAARQKRHNNIQDRLVKAASRCPGTISVNQTVVGVRGPDAALRPDIVVRDDVNRRVTIVDVAVPFENRLEAFDGVREAKIAKYTPLARQLTDS--GYTVTVEAFVVGALGAWDPRNERVLSLLSISRYY 327
[Close]
BLAST of SMED30001042 vs. TrEMBL
Match: A0A4U5MB42 (Uncharacterized protein OS=Steinernema carpocapsae OX=34508 GN=L596_025970 PE=4 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 6.237e-16
Identity = 65/207 (31.40%), Postives = 113/207 (54.59%), Query Frame = 3 
Query:  252 IASAIKLSPKINKAIYRGDISEF-AWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKKSNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNF-ALVDLLN--LQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLL 860
            + +A+ +S   +K +  G    F A+ +IHRAR N   +NA      + R CR C  E ET+ H+IQ CK  +TL ++RHN   D + + + KK+   V  +     +P S  R D+++ D   K++ ++D+  P +  N + AL D  N  + KY+S K +++     +KV L T +IGSLG++  K    L ++GV +++ + ++
Sbjct:  268 VLAAVSMSESSSKFLRDGLYVSFSAYKWIHRARLNLHRLNATSFGISDNR-CRRC-GEKETLPHVIQHCKQAMTLITKRHNAVQDRLVAAIPKKAGRTVTTN---VSIPISSNRPDIVVKDDQSKEVIIIDVTVPFE--NGYKALKDAYNRKIAKYESEKKKLESE--GYKVTLTTFVIGSLGTWFSKNTHCLKELGVRKSYTRKMI 465
[Close]
BLAST of SMED30001042 vs. Ensembl Medaka
Match: ENSORLT00000027781.1 (pep primary_assembly:ASM223467v1:7:9724985:9727820:1 gene:ENSORLG00000028072.1 transcript:ENSORLT00000027781.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.0694 bits (131), Expect = 1.646e-7
Identity = 46/180 (25.56%), Postives = 79/180 (43.89%), Query Frame = 3 
Query:  351 NTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGS-ERHNDCLDLISSHLSKKSNL-----------IVVVDHVCSLVPESKERVDLM---------ITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKI 827
            +T +I A+ ++      CRLC    ET+ HI   CK+       ERHN    +I  ++  +  L            VV +    ++ + + + D M         + D  ++K  +VD+  P D   N    +   L+KY  L+ +++ A    KV +   +IG+LG+  PK  E L +I
Sbjct:  665 STRAIEAQIYHTRQDPRCRLCKEAPETIQHITAGCKMLAGKAYMERHNQVAGIIYRNMCAEYGLETPRSKWETPPKVVENERAKILWDFQIQTDRMVMANQPDIVVVDKEQRKAVVVDVAVPSD--GNIRKKEHEKLEKYQGLREELEKAW-KMKVTVVPVVIGALGAVTPKLEEWLQQI 841
[Close]
BLAST of SMED30001042 vs. Planmine SMEST
Match: SMESG000044750.1 (SMESG000044750.1)

HSP 1 Score: 647.121 bits (1668), Expect = 0.000e+0
Identity = 316/325 (97.23%), Postives = 316/325 (97.23%), Query Frame = 3 
Query:    3 INGKEPK-------TRFQRARIAIAFF*KEHDINIMFIVTKGQPSLFITSEKRGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKKSNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLLKDCALSNISHSARRWHYHKTGILVNLEKFRFN 956
            INGKEPK       TRFQRARIAIAFF KEHDINIMFIVTKGQPSLFITSEKRGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSK SNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLLKDCALSNISHSARRWHYHKTGILVNLEKFRFN
Sbjct: 2067 INGKEPKPWHSGKKTRFQRARIAIAFFKKEHDINIMFIVTKGQPSLFITSEKRGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKNSNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLLKDCALSNISHSARRWHYHKTGILVNLEKFRFN 2391

HSP 2 Score: 576.63 bits (1485), Expect = 0.000e+0
Identity = 285/300 (95.00%), Postives = 287/300 (95.67%), Query Frame = 3 
Query:    3 INGKEPKTRFQRARIAIAFF*KEHDINIMFIVTKGQPSLFITSEKRGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKKSNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLLKDCALSNISHSARR 902
            INGKEPK       + I    KEHDINIMFIVTKGQPSLFITSEKRGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSK SNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLK+QIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLLKDCALSNISHSARR
Sbjct: 1711 INGKEPK-------LGIL---KEHDINIMFIVTKGQPSLFITSEKRGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKNSNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKIQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLLKDCALSNISHSARR 2000
[Close]
BLAST of SMED30001042 vs. Planmine SMEST
Match: SMESG000046455.1 (SMESG000046455.1)

HSP 1 Score: 624.78 bits (1610), Expect = 0.000e+0
Identity = 303/316 (95.89%), Postives = 304/316 (96.20%), Query Frame = 3 
Query:    3 INGKEPK-------TRFQRARIAIAFF*KEHDINIMFIVTKGQPSLFITSEKRGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKKSNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLLKDCALSNISHSARRWHYHKTGIL 929
            INGKEPK       TRFQRARIAIAFF KEHDINIMFIVT G+PSLFITSEK GTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSK SNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLL DCALSNISHSARRWHYHKTGIL
Sbjct: 1970 INGKEPKPRHSGKKTRFQRARIAIAFFKKEHDINIMFIVTNGKPSLFITSEKGGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKNSNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLLIDCALSNISHSARRWHYHKTGIL 2285
[Close]
BLAST of SMED30001042 vs. Planmine SMEST
Match: SMESG000046455.1 (SMESG000046455.1)

HSP 1 Score: 573.548 bits (1477), Expect = 0.000e+0
Identity = 280/302 (92.72%), Postives = 283/302 (93.71%), Query Frame = 3 
Query:    3 INGKEPK-------TRFQRARIAIAFF*KEHDINIMFIVTKGQPSLFITSEKRGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKKSNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLLKDCALSNIS 887
            INGKEPK       TRFQRARIAIAFF KEHDINIMFIVT G+PSLFITSEK GTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSK SNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLK +L       I+
Sbjct: 2027 INGKEPKPRHSGKKTRFQRARIAIAFFKKEHDINIMFIVTNGKPSLFITSEKGGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKNSNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKDVLFSLLFGGIA 2328
[Close]
BLAST of SMED30001042 vs. Planmine SMEST
Match: SMESG000021486.1 (SMESG000021486.1)

HSP 1 Score: 516.153 bits (1328), Expect = 8.180e-164
Identity = 251/324 (77.47%), Postives = 280/324 (86.42%), Query Frame = 3 
Query:    6 NGKEPK-------TRFQRARIAIAFF*KEHDINIMFIVTKGQPSLFITSEKRGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKKSNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLLKDCALSNISHSARRWHYHKTGILVNLEKFRFN 956
            NGKE K       TRFQRARIA+AFF K H+I++ FIV   +PSL+IT+  RGT ILT DLRKTTSKVLHMALCDSYLSKWEK C SNFIASA+KLSPKINKAI+R ++SEFAW+FIHRARTNTLSI AK HNKG+KRLCRLCH EDETMSH IQSCK+HLTLG+ERHNDCL LI+S+L+K SNLIVVVDHVCSLVP SKERVDLMITD+ RKKIFLVDMKCPCD++NNF  VDLLNL+KYDSLK QIQ  KPDFKVELDTC+ GSLGSFP KT E+L+KIGV RT LK LLK+CALSNI+HSARRWH+HKTGILV+ EK   N
Sbjct: 3540 NGKESKPHHSGRKTRFQRARIAVAFFEKTHNISVSFIVKDHKPSLYITTAMRGTIILTSDLRKTTSKVLHMALCDSYLSKWEKSCVSNFIASAVKLSPKINKAIFRSELSEFAWNFIHRARTNTLSIFAKHHNKGDKRLCRLCHAEDETMSHAIQSCKVHLTLGAERHNDCLKLIASNLTKNSNLIVVVDHVCSLVPNSKERVDLMITDLSRKKIFLVDMKCPCDSVNNFEQVDLLNLRKYDSLKNQIQAVKPDFKVELDTCVFGSLGSFPLKTSEILVKIGVKRTELKPLLKECALSNIAHSARRWHFHKTGILVDPEKIYKN 3863

HSP 2 Score: 516.153 bits (1328), Expect = 8.584e-164
Identity = 250/320 (78.12%), Postives = 279/320 (87.19%), Query Frame = 3 
Query:    6 NGKEPK-------TRFQRARIAIAFF*KEHDINIMFIVTKGQPSLFITSEKRGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKKSNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLLKDCALSNISHSARRWHYHKTGILVNLEK 944
            NGKE K       TRFQRARIA+AFF K H+I++ FIV   +PSL+IT+  RGT ILT DLRKTTSKVLHMALCDSYLSKWEK C SNFIASA+KLSPKINKAI+R ++SEFAW+FIHRARTNTLSI AK HNKG+KRLCRLCH EDETMSH IQSCK+HLTLG+ERHNDCL LI+S+L+K SNLIVVVDHVCSLVP SKERVDLMITD+ RKKIFLVDMKCPCD++NNF  VDLLNL+KYDSLK QIQ  KPDFKVELDTC+ GSLGSFP KT E+L+KIGV RT LK LLK+CALSNI+HSARRWH+HKTGILV+ EK
Sbjct: 5896 NGKESKPHHSGRKTRFQRARIAVAFFEKTHNISVSFIVKDHKPSLYITTAMRGTIILTSDLRKTTSKVLHMALCDSYLSKWEKSCVSNFIASAVKLSPKINKAIFRSELSEFAWNFIHRARTNTLSIFAKHHNKGDKRLCRLCHAEDETMSHAIQSCKVHLTLGAERHNDCLKLIASNLTKNSNLIVVVDHVCSLVPNSKERVDLMITDLSRKKIFLVDMKCPCDSVNNFEQVDLLNLRKYDSLKNQIQAVKPDFKVELDTCVFGSLGSFPLKTSEILVKIGVKRTELKPLLKECALSNIAHSARRWHFHKTGILVDPEK 6215

HSP 3 Score: 342.043 bits (876), Expect = 1.688e-103
Identity = 177/323 (54.80%), Postives = 226/323 (69.97%), Query Frame = 3 
Query:    3 INGKEPK-------TRFQRARIAIAFF*KEHDINIMFIVTKGQPSLFITSEKRGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKKSNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLLKDCALSNISHSARRWHYHKTGILVNLEKFR 950
            INGKE K       TRF R R +I +F + H+I ++F + K  PS+ IT+   GT I+ P LRKT SK+LH AL DSYLSKWE    SN + SAIKL P +NK I+R D+++F W FIHRARTNTLS  AKPH  G  RLCR C +EDET+ H++Q+CKI+ +L + RHN CL  I   + K   L+VVVDH CSLVP S ERVDL+IT+  +K I LVDMKCP D+++NF LVD LNL+KY  LK+ IQ  KPD+KVEL TCI+GSLGS   KT +LLLK+GV    + GL+K+CA+SNI+HSA+  + ++    VN E F+
Sbjct: 1220 INGKESKLNHSGRKTRFHRLRTSIEYFSRVHNIKLLFEIFKQNPSIRITTSLTGTRIILPKLRKTLSKILHSALQDSYLSKWEMSNHSNLLVSAIKLCPLVNKLIFRSDLTDFEWKFIHRARTNTLSTFAKPHKAGEDRLCRRCKSEDETILHVLQTCKINQSLATNRHNACLIKIKDSI-KDPELLVVVDHKCSLVPTSSERVDLIITNTEKKTILLVDMKCPIDSVSNFQLVDNLNLEKYAKLKLDIQAVKPDYKVELHTCIMGSLGSIESKTEDLLLKMGVQPNRIIGLMKECAISNIAHSAKICYKNE----VNCENFK 1537
[Close]
BLAST of SMED30001042 vs. Planmine SMEST
Match: SMESG000021486.1 (SMESG000021486.1)

HSP 1 Score: 515.768 bits (1327), Expect = 9.629e-164
Identity = 251/324 (77.47%), Postives = 280/324 (86.42%), Query Frame = 3 
Query:    6 NGKEPK-------TRFQRARIAIAFF*KEHDINIMFIVTKGQPSLFITSEKRGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKKSNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLLKDCALSNISHSARRWHYHKTGILVNLEKFRFN 956
            NGKE K       TRFQRARIA+AFF K H+I++ FIV   +PSL+IT+  RGT ILT DLRKTTSKVLHMALCDSYLSKWEK C SNFIASA+KLSPKINKAI+R ++SEFAW+FIHRARTNTLSI AK HNKG+KRLCRLCH EDETMSH IQSCK+HLTLG+ERHNDCL LI+S+L+K SNLIVVVDHVCSLVP SKERVDLMITD+ RKKIFLVDMKCPCD++NNF  VDLLNL+KYDSLK QIQ  KPDFKVELDTC+ GSLGSFP KT E+L+KIGV RT LK LLK+CALSNI+HSARRWH+HKTGILV+ EK   N
Sbjct: 3511 NGKESKPHHSGRKTRFQRARIAVAFFEKTHNISVSFIVKDHKPSLYITTAMRGTIILTSDLRKTTSKVLHMALCDSYLSKWEKSCVSNFIASAVKLSPKINKAIFRSELSEFAWNFIHRARTNTLSIFAKHHNKGDKRLCRLCHAEDETMSHAIQSCKVHLTLGAERHNDCLKLIASNLTKNSNLIVVVDHVCSLVPNSKERVDLMITDLSRKKIFLVDMKCPCDSVNNFEQVDLLNLRKYDSLKNQIQAVKPDFKVELDTCVFGSLGSFPLKTSEILVKIGVKRTELKPLLKECALSNIAHSARRWHFHKTGILVDPEKIYKN 3834

HSP 2 Score: 515.768 bits (1327), Expect = 1.001e-163
Identity = 250/320 (78.12%), Postives = 279/320 (87.19%), Query Frame = 3 
Query:    6 NGKEPK-------TRFQRARIAIAFF*KEHDINIMFIVTKGQPSLFITSEKRGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKKSNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLLKDCALSNISHSARRWHYHKTGILVNLEK 944
            NGKE K       TRFQRARIA+AFF K H+I++ FIV   +PSL+IT+  RGT ILT DLRKTTSKVLHMALCDSYLSKWEK C SNFIASA+KLSPKINKAI+R ++SEFAW+FIHRARTNTLSI AK HNKG+KRLCRLCH EDETMSH IQSCK+HLTLG+ERHNDCL LI+S+L+K SNLIVVVDHVCSLVP SKERVDLMITD+ RKKIFLVDMKCPCD++NNF  VDLLNL+KYDSLK QIQ  KPDFKVELDTC+ GSLGSFP KT E+L+KIGV RT LK LLK+CALSNI+HSARRWH+HKTGILV+ EK
Sbjct: 5838 NGKESKPHHSGRKTRFQRARIAVAFFEKTHNISVSFIVKDHKPSLYITTAMRGTIILTSDLRKTTSKVLHMALCDSYLSKWEKSCVSNFIASAVKLSPKINKAIFRSELSEFAWNFIHRARTNTLSIFAKHHNKGDKRLCRLCHAEDETMSHAIQSCKVHLTLGAERHNDCLKLIASNLTKNSNLIVVVDHVCSLVPNSKERVDLMITDLSRKKIFLVDMKCPCDSVNNFEQVDLLNLRKYDSLKNQIQAVKPDFKVELDTCVFGSLGSFPLKTSEILVKIGVKRTELKPLLKECALSNIAHSARRWHFHKTGILVDPEK 6157

HSP 3 Score: 342.043 bits (876), Expect = 1.687e-103
Identity = 177/323 (54.80%), Postives = 226/323 (69.97%), Query Frame = 3 
Query:    3 INGKEPK-------TRFQRARIAIAFF*KEHDINIMFIVTKGQPSLFITSEKRGTFILTPDLRKTTSKVLHMALCDSYLSKWEKGCTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNKGNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKKSNLIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVDLLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVDRTHLKGLLKDCALSNISHSARRWHYHKTGILVNLEKFR 950
            INGKE K       TRF R R +I +F + H+I ++F + K  PS+ IT+   GT I+ P LRKT SK+LH AL DSYLSKWE    SN + SAIKL P +NK I+R D+++F W FIHRARTNTLS  AKPH  G  RLCR C +EDET+ H++Q+CKI+ +L + RHN CL  I   + K   L+VVVDH CSLVP S ERVDL+IT+  +K I LVDMKCP D+++NF LVD LNL+KY  LK+ IQ  KPD+KVEL TCI+GSLGS   KT +LLLK+GV    + GL+K+CA+SNI+HSA+  + ++    VN E F+
Sbjct: 1220 INGKESKLNHSGRKTRFHRLRTSIEYFSRVHNIKLLFEIFKQNPSIRITTSLTGTRIILPKLRKTLSKILHSALQDSYLSKWEMSNHSNLLVSAIKLCPLVNKLIFRSDLTDFEWKFIHRARTNTLSTFAKPHKAGEDRLCRRCKSEDETILHVLQTCKINQSLATNRHNACLIKIKDSI-KDPELLVVVDHKCSLVPTSSERVDLIITNTEKKTILLVDMKCPIDSVSNFQLVDNLNLEKYAKLKLDIQAVKPDYKVELHTCIMGSLGSIESKTEDLLLKMGVQPNRIIGLMKECAISNIAHSAKICYKNE----VNCENFK 1537
The following BLAST results are available for this feature:
BLAST of SMED30001042 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001042 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001042 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001042 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001042 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001042 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001042 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001042 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
Q7YXU71.529e-1630.45ORF2 (Fragment) OS=Girardia tigrina OX=6162 PE=4 S... [more]
K7JS433.580e-1626.22C2H2-type domain-containing protein OS=Nasonia vit... [more]
K7JWY83.647e-1626.22C2H2-type domain-containing protein OS=Nasonia vit... [more]
K7JS443.716e-1626.22C2H2-type domain-containing protein OS=Nasonia vit... [more]
A0A4U5MB426.237e-1631.40Uncharacterized protein OS=Steinernema carpocapsae... [more]
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BLAST of SMED30001042 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001042 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001042 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001042 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30001042 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSORLT00000027781.11.646e-725.56pep primary_assembly:ASM223467v1:7:9724985:9727820... [more]
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BLAST of SMED30001042 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000044750.10.000e+097.23SMESG000044750.1[more]
SMESG000046455.10.000e+095.89SMESG000046455.1[more]
SMESG000046455.10.000e+092.72SMESG000046455.1[more]
SMESG000021486.18.180e-16477.47SMESG000021486.1[more]
SMESG000021486.19.629e-16477.47SMESG000021486.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001042 ID=SMED30001042|Name=SMED30001042|organism=Schmidtea mediterranea sexual|type=transcript|length=1596bp
GGATCAATGGGAAAGAGCCAAAAACTCGTTTTCAGCGAGCTAGAATTGCG
ATTGCTTTTTTTTAGAAAGAACATGATATTAATATTATGTTTATTGTCAC
CAAAGGTCAACCTTCACTCTTCATTACATCAGAAAAACGTGGGACATTTA
TTCTTACTCCTGATTTGCGTAAAACTACTTCAAAAGTCTTGCACATGGCT
CTCTGTGACTCGTATCTTTCGAAATGGGAAAAAGGCTGCACTTCAAATTT
CATTGCCTCAGCCATTAAACTTTCACCAAAAATTAATAAAGCTATTTATC
GAGGTGACATTTCTGAGTTTGCTTGGCACTTTATCCATCGTGCTCGTACA
AATACTTTGTCCATCAATGCAAAACCACATAATAAAGGTAATAAAAGACT
GTGTCGATTATGTCACACTGAAGACGAAACAATGTCGCATATTATTCAAT
CTTGTAAAATCCATTTGACTCTTGGTTCTGAGAGGCACAATGATTGTCTT
GATCTGATTTCGTCTCACTTGTCGAAAAAATCAAATTTGATTGTTGTTGT
TGACCATGTATGTTCACTTGTTCCGGAATCGAAAGAGCGAGTAGATTTAA
TGATCACTGACATGGTGCGGAAAAAAATATTTTTAGTTGACATGAAATGT
CCATGTGATACAATTAATAATTTTGCACTAGTAGATCTTCTTAATTTACA
AAAATACGATTCGTTGAAAATGCAAATACAAGATGCCAAACCTGACTTCA
AAGTGGAGCTTGATACTTGTATCATCGGCTCTTTAGGTTCCTTTCCTCCA
AAGACTCCTGAATTACTTTTAAAGATTGGAGTTGATCGAACTCACCTGAA
AGGTTTATTAAAAGATTGTGCGCTAAGCAATATTTCACATTCTGCTAGAC
GGTGGCACTACCACAAGACGGGTATTTTGGTAAATCTTGAAAAATTTCGA
TTTAATTAATGTTTGAGTTAAAATTGCCATTTGACTTGTGTATGTGATTT
ATTGTGTTAATTAATGAATTCCACCCTCTTCCAGGGAAAGGTTTAACTTT
GCATAAAATTAAACTCTCTGTCTACACTTAGTAGCAAATTAATCTAGAAT
GAAGAAGCCTGATGGTATTTTTCCCAAATCTGCTGAAAACTTAGTTCTGA
AGGATGGAAATAAAAGCAGATAAAGATTCAATATTCGATAAAGAAATGGA
CCTCAACAATCAATGTCAATTATCAATAATAAATCTGGGCAGATGTCAGA
AGGCGATAATCAAATGCTGAATTCCAATGACCGAATCTACTTTGATGTCT
TAGATTTGTGATCAGCTTCCACTACAAAGGTGCTGCATCAAAAGCCAATG
GAGATTCAATGCCGGATGAAAATGCCGATGAAAGAGAATCAGAGCCGAGG
ACAATTTAGAGAAAATTCGATTGCAACTGCGTCAAGAGTTCCTCAACAAT
AAGATGAAATATCGAAACTCAATTGGATCTTTTGCTTTTGGAGAGTCTCA
GAACGGCAAGCTCCATGTAGAGCTGTGTCCGATATGAAAAATTCAAAATT
GTTATATTAATAAATTTTTATATGAGTGATTTTGCTAAGTCTACGA
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protein sequence of SMED30001042-orf-1

>SMED30001042-orf-1 ID=SMED30001042-orf-1|Name=SMED30001042-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=291bp
MFIVTKGQPSLFITSEKRGTFILTPDLRKTTSKVLHMALCDSYLSKWEKG
CTSNFIASAIKLSPKINKAIYRGDISEFAWHFIHRARTNTLSINAKPHNK
GNKRLCRLCHTEDETMSHIIQSCKIHLTLGSERHNDCLDLISSHLSKKSN
LIVVVDHVCSLVPESKERVDLMITDMVRKKIFLVDMKCPCDTINNFALVD
LLNLQKYDSLKMQIQDAKPDFKVELDTCIIGSLGSFPPKTPELLLKIGVD
RTHLKGLLKDCALSNISHSARRWHYHKTGILVNLEKFRFN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002089reproductive organ
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
Vocabulary: molecular function
TermDefinition
GO:0008234cysteine-type peptidase activity