39S ribosomal protein L44, mitochondrial

Overview
Name39S ribosomal protein L44, mitochondrial
Smed IDSMED30001033
Length (bp)1006
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 39S ribosomal protein L44, mitochondrial (SMED30001033) t-SNE clustered cells

Violin plots show distribution of expression levels for 39S ribosomal protein L44, mitochondrial (SMED30001033) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 39S ribosomal protein L44, mitochondrial (SMED30001033) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 39S ribosomal protein L44, mitochondrial (SMED30001033) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30001033

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymaSMED30001033h1SMcG0012984 dd_Smed_v4_6197_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
testisSMED30001033h1SMcG0010257 Contig4475uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30001033h1SMcG0010257 Contig4475newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
prepharyngeal regionSMED30001033h1SMcG0010257 Contig4475uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
prepharyngeal regionSMED30001033h1SMcG0010257 Contig4475newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30001033h1SMcG0012984 Contig4475uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30001033h1SMcG0012984 Contig4475newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
prepharyngeal regionSMED30001033h1SMcG0012984 Contig4475uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
prepharyngeal regionSMED30001033h1SMcG0012984 Contig4475newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Human
Match: MRPL44 (mitochondrial ribosomal protein L44 [Source:HGNC Symbol;Acc:HGNC:16650])

HSP 1 Score: 145.591 bits (366), Expect = 2.523e-40
Identity = 98/297 (33.00%), Postives = 146/297 (49.16%), Query Frame = 3 
Query:   81 VRYVRDITKRLQ-----NQEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQ-------------DMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDFEDKFE 917
            V  VR + K  +      +E   +R+   P    +RSE  NW+Y +EI AFG RL+ENF +  L+ AF++  YI S E +             ++ +N EL   G S S      FL   +PD+P+E I  L + L    +  HV+ +L  + L       +         FA+IGAL +S        FI+DF++TQ+T   L +++++ N   LL   L      AP  RL RQSG  +    Y VG+Y D  L+ E  GE++L AE+ AAR +L  L+G +ENR   ++    E
Sbjct:   25 VPPVRGVKKGFRAAFRFQKELERQRLLRCPPPPVRRSEKPNWDYHAEIQAFGHRLQENFSLDLLKTAFVNSCYIKSEEAKRQQLGIEKEAVLLNLKSNQELSEQGTSFSQTCLTQFLEDEYPDMPTEGIKNLVDFLTGEEVVCHVARNLAVEQLTLSEEFPVPPAVLQQTFFAVIGALLQSSGPERTALFIRDFLITQMTGKELFEMWKIINPMGLLVEELKKRNVSAPESRLTRQSGGTTALPLYFVGLYCDKKLIAEGPGETVLVAEEEAARVALRKLYGFTENRRPWNYSKPKE 321
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Celegans
Match: mrpl-44 (Mitochondrial Ribosomal Protein, Large [Source:RefSeq peptide;Acc:NP_499012])

HSP 1 Score: 100.138 bits (248), Expect = 1.650e-23
Identity = 82/285 (28.77%), Postives = 142/285 (49.82%), Query Frame = 3 
Query:   78 SVRYVRDITKRLQNQEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRL--KENFDIKYLRQAFIDKSYINSNETQDML------------NNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDN-DXXXXXXXXXXXXXXDMAARHSLLNLFGVSENR 887
            S R +R   +R   ++ Y+RR  +G + A  RS + NW+Y+SE++AFG R+   E  + +Y++ A  ++S+    + ++ +            +N EL   G    S + + +L       P E I  + +HLL     A ++ HLG   L+      +  E+ +D   A+ G  +   DE V    + DFI+ QL      D++ + +   +++ +L  +      PR+LR +G NS    Y V IY+D    +G++AGESL  A DMAAR +LL L+ ++  +
Sbjct:   18 SYRLLRTRWERGYLKDLYHRRQILGADPAISRSSYPNWDYDSELYAFGHRIGAPEISEDQYIK-ALTNESFFQRADVEENVESAQPGAEVGTEHNAELVKRGEQNLSIWLKRYLRFHLAKAPEELIEAIDSHLLDDECLAGIASHLGIDHLVRTKEFPISQESSADAFRALAGVFS---DEKVK-NLVIDFIVPQLVDIDFADIYPLADPLAVVTDLLKSNGVTEIEPRVLRSAGENSAEPIYVVAIYADKLKNVGQSAGESLAIAVDMAAREALLRLWDITSEK 297
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Fly
Match: mRpL44 (gene:FBgn0037330 transcript:FBtr0078768)

HSP 1 Score: 180.644 bits (457), Expect = 3.939e-54
Identity = 114/314 (36.31%), Postives = 168/314 (53.50%), Query Frame = 3 
Query:   42 MSFLKSNLPIKISVRYV--------RDITKRLQN---QEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQ-----------DMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDFEDKFE 917
            MSFL+S + +   V+ +        R   KR  +   +E  +R+ ++GP+K  +RS F  WN  SE+FAFGKRL E+F++  L++AF +KS+    E +            M +N++L   G  ++  Y  AFL H  P +P+E +  + ++LLS    AHVS HLGTKDLI         E+++  L A+IGALA S     AF F++DFI TQL    L +V+       LL  +    + G   PRLL   G N+V A Y VGIY++  LLG+  GE +  A + AA  +L ++F   +NR   DF  + E
Sbjct:    1 MSFLRSAVGLLARVKLLEGSPTVQTRRHIKRWVSPTLRELAHRQKKLGPQKPERRSGFVEWNQRSELFAFGKRLGESFELSELQRAFTEKSFAEREEDRRRQLGIEESDLQMPHNSDLVEKGQQIARAYVEAFLQHQLPKVPNEGLQAIASYLLSTETLAHVSTHLGTKDLIQSTEYPPSAESQAKSLHAVIGALASSSGIERAFIFVRDFICTQLNQKDLLEVWTPQEPIQLLEKICQERKLGEAEPRLLGDCGKNTVLAAYQVGIYANRQLLGKGFGEDVKTATETAALDALQSIFDTRDNRRPFDFAIQLE 314
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Zebrafish
Match: mrpl44 (mitochondrial ribosomal protein L44 [Source:ZFIN;Acc:ZDB-GENE-050913-59])

HSP 1 Score: 145.206 bits (365), Expect = 2.044e-40
Identity = 90/264 (34.09%), Postives = 143/264 (54.17%), Query Frame = 3 
Query:  150 GPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQ-------------DMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDF 902
            GP   + RSE  N++Y +E+ AFG RL+E+F +  L+ AF++  Y+ S   +             ++ +NTELR++G   ++ +   +  +SFP+LP E +  +  HL    +  HV+ +L  ++L       +         FA+IGAL +S     A  FI+DF++TQL    L D+++V +   LL   L       P PRL+R +G ++V   Y VG+YSD  LL +  GE++L AE+ AAR +L  L+G +ENR   DF
Sbjct:   51 GPPAPKPRSEQPNFDYHAEVEAFGVRLQESFSVDLLKTAFVNSCYLRSEVERRKALGLDSESAILNLKDNTELRDHGQHFTTGFLSDWCKNSFPNLPDEGVAAIAGHLTGSEVICHVARNLAVEELTMSAEFPVPDHILQGTFFAVIGALEQSSGPVRAGLFIRDFLVTQLIGKDLFDMWKVVDPMGLLVEELTRKGVALPEPRLIRSAGASTVLPLYFVGLYSDRKLLAQGPGETVLIAEEEAARVALRKLYGFTENRRPFDF 314
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Zebrafish
Match: mrpl44 (mitochondrial ribosomal protein L44 [Source:ZFIN;Acc:ZDB-GENE-050913-59])

HSP 1 Score: 80.4925 bits (197), Expect = 6.162e-18
Identity = 52/121 (42.98%), Postives = 73/121 (60.33%), Query Frame = 3 
Query:  540 FAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDF 902
            FA+IGAL +S     A  FI+DF++TQL    L D+++V +   LL   L       P PRL+R +G ++V   Y VG+YSD  LL +  GE++L AE+ AAR +L  L+G +ENR   DF
Sbjct:   17 FAVIGALEQSSGPVRAGLFIRDFLVTQLIGKDLFDMWKVVDPMGLLVEELTRKGVALPEPRLIRSAGASTVLPLYFVGLYSDRKLLAQGPGETVLIAEEEAARVALRKLYGFTENRRPFDF 137
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Xenopus
Match: mrpl44 (mitochondrial ribosomal protein L44 [Source:NCBI gene;Acc:496841])

HSP 1 Score: 150.599 bits (379), Expect = 2.498e-42
Identity = 103/293 (35.15%), Postives = 156/293 (53.24%), Query Frame = 3 
Query:   63 LPIKISVRYVRDITKRLQNQEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQ----------DMLN---NTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDF 902
            +P++   R+++   + L+ Q     R   GP +   RS+  N++Y +E+ AF +RL E+F I+ L+ AF++K+Y+   E +           MLN   N EL   G   ++ Y +  L H FP+LP + I  L +HL S  +  HV+ +L  +DL   +   LD  T     FAIIGAL +S     A  FI+DF+L QL    L D++ + +  +LL   L +     P PRL R+ G  +V   Y VG+Y D  L+ E  GES+ AAE+ AAR +L  +FG +ENR   D+
Sbjct:   19 IPVRGKKRWLQPYLRALERQ-----REVAGPPEPIPRSQQPNFDYHAEVLAFSQRLNESFSIQLLKTAFVNKTYVEQEEQRRQELGLDKEMAMLNLQDNEELAGVGSDFTATYLQKTLEHGFPNLPPDGIKSLVDHLSSQEVINHVAQNLAVEDLALGSECPLDPLTVYRTFFAIIGALLQSSGTQRAGLFIRDFLLAQLIGKDLFDIWLIADPMSLLVQELSHRNLPLPEPRLTRECGAATVLPVYFVGLYCDKKLIAEGPGESISAAEEEAARVALRKMFGFTENRRPWDY 306
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Mouse
Match: Mrpl44 (mitochondrial ribosomal protein L44 [Source:MGI Symbol;Acc:MGI:1916413])

HSP 1 Score: 150.214 bits (378), Expect = 3.323e-42
Identity = 103/295 (34.92%), Postives = 145/295 (49.15%), Query Frame = 3 
Query:   90 VRDITK------RLQNQEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQ-------------DMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDFEDKFE 917
            VR + K      R Q +   +R +   P   R RSE  NW+Y +E+ AFG RL+E F +  L+ AFI+  YI S E +             ++ +N EL   G S S +    FL   FPDLP+E    L + L    +  HV+ +L  + L       + L       FA+IGAL +S     A  FI+DF++TQ+T   L +++ V N   LL   L      AP  RL RQSG  +    Y VG+Y D  L+ E  GE++L AE+ AAR +L  L+G +ENR   D+    E
Sbjct:   28 VRGVKKGFRAAFRFQKELERWRLLRCPPPPVR-RSEKPNWDYHAEVQAFGSRLQETFSLDLLKTAFINSCYIKSEEAKRQSLGIEKEAALLNLKDNQELFEQGLSFSHRCLTQFLEDEFPDLPAEGTESLVSFLTGEAVVCHVARNLAVEQLTLSAEFPVPLPVLRQTFFAVIGALLQSSGPERAALFIRDFLITQMTGKELFEMWTVVNPMGLLVEELKKRNISAPESRLTRQSGSTTALPLYFVGLYCDRKLIAEGPGETVLVAEEEAARVALRKLYGFTENRRPWDYSKPKE 321
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. UniProt/SwissProt
Match: sp|Q9CY73|RM44_MOUSE (39S ribosomal protein L44, mitochondrial OS=Mus musculus OX=10090 GN=Mrpl44 PE=1 SV=3)

HSP 1 Score: 150.214 bits (378), Expect = 2.324e-41
Identity = 103/295 (34.92%), Postives = 145/295 (49.15%), Query Frame = 3 
Query:   90 VRDITK------RLQNQEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQ-------------DMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDFEDKFE 917
            VR + K      R Q +   +R +   P   R RSE  NW+Y +E+ AFG RL+E F +  L+ AFI+  YI S E +             ++ +N EL   G S S +    FL   FPDLP+E    L + L    +  HV+ +L  + L       + L       FA+IGAL +S     A  FI+DF++TQ+T   L +++ V N   LL   L      AP  RL RQSG  +    Y VG+Y D  L+ E  GE++L AE+ AAR +L  L+G +ENR   D+    E
Sbjct:   28 VRGVKKGFRAAFRFQKELERWRLLRCPPPPVR-RSEKPNWDYHAEVQAFGSRLQETFSLDLLKTAFINSCYIKSEEAKRQSLGIEKEAALLNLKDNQELFEQGLSFSHRCLTQFLEDEFPDLPAEGTESLVSFLTGEAVVCHVARNLAVEQLTLSAEFPVPLPVLRQTFFAVIGALLQSSGPERAALFIRDFLITQMTGKELFEMWTVVNPMGLLVEELKKRNISAPESRLTRQSGSTTALPLYFVGLYCDRKLIAEGPGETVLVAEEEAARVALRKLYGFTENRRPWDYSKPKE 321
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. UniProt/SwissProt
Match: sp|Q2KIS2|RM44_BOVIN (39S ribosomal protein L44, mitochondrial OS=Bos taurus OX=9913 GN=MRPL44 PE=2 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 1.150e-39
Identity = 97/290 (33.45%), Postives = 142/290 (48.97%), Query Frame = 3 
Query:   90 VRDITK------RLQNQEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQ-------------DMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDF 902
            VR + K      R Q +   +R +   P   R RSE  NW+Y +EI AFG RL+E F +  L+ AF++  YI S E +             ++ +N EL   G S S      F   +FPDLP+E +  L ++L    +  HV+ +L  + L       +         FA+IGAL  S        FI+DF++TQ+T   L +++++ N   LL   L       P  RL RQSG  +    Y VG+Y D  L+ E  GE++L AE+ AAR +L  L+G +ENR   D+
Sbjct:   28 VRGVKKGFRAAFRFQKELERWRLLRCPPPPVR-RSEKPNWDYHAEIQAFGPRLQETFSLDLLKTAFVNSCYIKSEEAKRQKLGIEKEAVLLNLKDNQELSEQGTSFSQTCLTQFFEDAFPDLPTEGVKSLVDYLTGEEVVCHVARNLAVEQLTLSADFPVPPAVLRQTFFAVIGALLHSSGPERTSLFIRDFLITQMTGKELFEIWKIINPMGLLVQELKKRNISVPESRLTRQSGSTTALPVYFVGLYCDKKLIAEGPGETVLVAEEEAARVALRKLYGFTENRQPWDY 316
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. UniProt/SwissProt
Match: sp|Q9H9J2|RM44_HUMAN (39S ribosomal protein L44, mitochondrial OS=Homo sapiens OX=9606 GN=MRPL44 PE=1 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 1.212e-39
Identity = 98/297 (33.00%), Postives = 146/297 (49.16%), Query Frame = 3 
Query:   81 VRYVRDITKRLQ-----NQEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQ-------------DMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDFEDKFE 917
            V  VR + K  +      +E   +R+   P    +RSE  NW+Y +EI AFG RL+ENF +  L+ AF++  YI S E +             ++ +N EL   G S S      FL   +PD+P+E I  L + L    +  HV+ +L  + L       +         FA+IGAL +S        FI+DF++TQ+T   L +++++ N   LL   L      AP  RL RQSG  +    Y VG+Y D  L+ E  GE++L AE+ AAR +L  L+G +ENR   ++    E
Sbjct:   25 VPPVRGVKKGFRAAFRFQKELERQRLLRCPPPPVRRSEKPNWDYHAEIQAFGHRLQENFSLDLLKTAFVNSCYIKSEEAKRQQLGIEKEAVLLNLKSNQELSEQGTSFSQTCLTQFLEDEYPDMPTEGIKNLVDFLTGEEVVCHVARNLAVEQLTLSEEFPVPPAVLQQTFFAVIGALLQSSGPERTALFIRDFLITQMTGKELFEMWKIINPMGLLVEELKKRNVSAPESRLTRQSGGTTALPLYFVGLYCDKKLIAEGPGETVLVAEEEAARVALRKLYGFTENRRPWNYSKPKE 321
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. UniProt/SwissProt
Match: sp|Q5REY8|RM44_PONAB (39S ribosomal protein L44, mitochondrial OS=Pongo abelii OX=9601 GN=MRPL44 PE=2 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 2.399e-39
Identity = 95/280 (33.93%), Postives = 139/280 (49.64%), Query Frame = 3 
Query:  117 NQEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQ-------------DMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDFEDKFE 917
             +E   +R+   P    +RSE  NW+Y +EI AFG RL+ENF +  L+ AF++  YI S E +             ++ +N EL   G S S      FL   +PDLP+E I  L   L    +  HV+ +L  + L       +         FA+IGAL +S        FI+DF++TQ+T   L +++++ N   LL   L      AP  RL RQSG  +    Y VG+Y D  L+ E  GE++L AE+ AAR +L  L+G +ENR   ++    E
Sbjct:   42 QKELERQRLLRCPPPPVRRSEKPNWDYHAEIQAFGHRLQENFSLDLLKTAFVNSCYIKSEEAKRQQLGIEKEAVLLNLKSNQELSEQGTSFSQTCLTQFLEDEYPDLPTEGIKNLVEFLTGEDVVCHVARNLAVEQLTLSEEFPVPPTVLQQTFFAVIGALLQSSGPERTALFIRDFLITQMTGKELFEMWKIINPMGLLVEELKKRNISAPESRLTRQSGGTTALPLYFVGLYCDKKLIAEGPGETVLVAEEEAARVALRKLYGFTENRRPWNYSKPKE 321
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. UniProt/SwissProt
Match: sp|P34384|RM44_CAEEL (Ribosomal protein L44, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=mrpl-44 PE=3 SV=3)

HSP 1 Score: 100.138 bits (248), Expect = 2.098e-22
Identity = 82/285 (28.77%), Postives = 142/285 (49.82%), Query Frame = 3 
Query:   78 SVRYVRDITKRLQNQEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRL--KENFDIKYLRQAFIDKSYINSNETQDML------------NNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDN-DXXXXXXXXXXXXXXDMAARHSLLNLFGVSENR 887
            S R +R   +R   ++ Y+RR  +G + A  RS + NW+Y+SE++AFG R+   E  + +Y++ A  ++S+    + ++ +            +N EL   G    S + + +L       P E I  + +HLL     A ++ HLG   L+      +  E+ +D   A+ G  +   DE V    + DFI+ QL      D++ + +   +++ +L  +      PR+LR +G NS    Y V IY+D    +G++AGESL  A DMAAR +LL L+ ++  +
Sbjct:   18 SYRLLRTRWERGYLKDLYHRRQILGADPAISRSSYPNWDYDSELYAFGHRIGAPEISEDQYIK-ALTNESFFQRADVEENVESAQPGAEVGTEHNAELVKRGEQNLSIWLKRYLRFHLAKAPEELIEAIDSHLLDDECLAGIASHLGIDHLVRTKEFPISQESSADAFRALAGVFS---DEKVK-NLVIDFIVPQLVDIDFADIYPLADPLAVVTDLLKSNGVTEIEPRVLRSAGENSAEPIYVVAIYADKLKNVGQSAGESLAIAVDMAAREALLRLWDITSEK 297
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. TrEMBL
Match: A0A2C9KAB1 (RNase III domain-containing protein OS=Biomphalaria glabrata OX=6526 GN=106068572 PE=4 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 8.348e-57
Identity = 120/311 (38.59%), Postives = 180/311 (57.88%), Query Frame = 3 
Query:  120 QEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQ-------------DMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTI---DFEDKFEIDFNKKPSYSIADYLKCFENK*YFSFLKK 1004
            +E Y RR+E+GPEK R+RSE+ NWNY+SEIFAFGKRL E F+   ++QAFI  SY+     +             D+ +N+EL   G  L S Y + +L + +P    E I  + N+L+S  + +H++ H+G  DLI      +   T S    A++GA+ K + +  A TF++DF+L QL    + ++++V N   LL+ VL    KG P PRLL +SG N++ A Y VGIYSD  L+ ++ GE++  AE+MAAR +L  L    ++RS +      D  ++D+ KK + S A+ LK      Y+S LKK
Sbjct:   30 REMYKRRLEVGPEKERRRSEWINWNYDSEIFAFGKRLGEEFNETTIKQAFIHSSYVEKERAKRAELGLNLKDLTFDLEDNSELAEKGADLISNYVKVYLRNMYPYFFEEGICAIHNYLVSESMLSHIAKHIGCGDLILSEDFPVLPSTLSQTFKAVVGAVLKDRGQEKAETFVRDFVLPQLISKDINELWEVVNPMGLLTAVLALIGKGPPEPRLLWKSGANTIMALYWVGIYSDKQLVAKSPGETVTIAEEMAAREALKKLMKTEDSRSPLILGRMADNLKLDY-KKVNMSAAEVLKN-----YYSQLKK 334
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. TrEMBL
Match: A0A0R3SCF8 (RNase III domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS2264 PE=4 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 9.644e-54
Identity = 129/330 (39.09%), Postives = 180/330 (54.55%), Query Frame = 3 
Query:   54 KSNLPIKISVRYVRDITKRLQNQEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDK---SYINSNETQDML-NNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFY--------NSDTLDLETE-------SDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQV---NNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDFEDKFEIDFNKKPSYSIADYLKCFEN 977
            K+N  +  SVR  R +   L+N   Y RR+  GPE  R RS +  WNY+SEI AF  R+ EN D   L   F DK   SY NS +    L +N++L   G S+S  Y R FL   +P LP EWI+ +CN LLS    + V  HLG  D++ Y        +S  L +  +       +    A+IGA+A   D+  A  FI+DFILT+L     C  F     +  F LL+GVL ++++G P PRL +QS MN++ ACY VGIYS+  L+GEAAGE++  AE  AA  +L NLFG+ +NR  +     +    +  P+ ++ DYL+  E 
Sbjct:    2 KTNYIVSRSVRIARYVRPYLRN--LYDRRLVQGPEPYRPRSAWKPWNYDSEILAFKNRIGENIDTNVLCLCFTDKSFSSYTNSKDASGHLRDNSKLAEQGKSVSELYIRGFLRKFYPRLPEEWISCICNRLLSDSELSCVGSHLGITDVLQYSLEEKRFDDSPLLPVINQPPPNSAIATSFLALIGAIA--NDQKAADLFIRDFILTRLLTLEFCSDFPFPLQDEPFKLLAGVLRFYKRGPPEPRLQKQSSMNTLLACYQVGIYSNCQLIGEAAGETIDVAEKEAALQALRNLFGIQDNRPWLPLSGTYVSVDDIPPNPTLEDYLRTEEQ 327
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. TrEMBL
Match: B4KD97 (RNase III domain-containing protein OS=Drosophila mojavensis OX=7230 GN=Dmoj\GI24499 PE=4 SV=1)

HSP 1 Score: 184.882 bits (468), Expect = 2.310e-52
Identity = 113/277 (40.79%), Postives = 154/277 (55.60%), Query Frame = 3 
Query:  120 QEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINS-NETQDML----------NNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDFEDKFE 917
            +E  +R  + GP+K   RS F  WNY SE+FAFGKRL E F +  L+ AF  ++Y N   E Q  L          +N +L+  G  ++  Y  A+L HS P LP      + ++LLS    AHVS HLGTK+LIF      + ET ++ L AIIGALA S   + AF F++DFI TQL    L D++       LLS +    + G P PRLL   G N++ A + VGIY+D  LLG+  GE++  A + AA  +LL LF + ENR   DF+ + E
Sbjct:   38 RELAHRVKKQGPQKPEPRSGFLEWNYRSELFAFGKRLNEEFQLPILQIAFTQQAYANQEQERQRKLGIAEADLQISDNKQLKAKGAHITRAYVEAYLQHSLPKLPPAGQKAITSYLLSTETLAHVSMHLGTKELIFSTEYPPNSETLAETLQAIIGALADSSGVDRAFLFVRDFICTQLNQKDLLDIWTPEQPLELLSEICNQRKLGEPEPRLLGDCGKNTLLAAFQVGIYADRQLLGKGFGENVQTATETAAICALLTLFDMHENRRPFDFQIQLE 314
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. TrEMBL
Match: B3LZH8 (RNase III domain-containing protein OS=Drosophila ananassae OX=7217 GN=Dana\GF17708 PE=4 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 3.739e-52
Identity = 114/302 (37.75%), Postives = 161/302 (53.31%), Query Frame = 3 
Query:   51 LKSNLPIKISVRYVRDITKRLQN--QEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQD-----------MLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDFEDKFE 917
            L  +L   I+V+  R I + +    +E  +R+ ++GP+K   R+ F  WN  SE+FAFGKRL E+F +  L++AF +K++    E +            +L+N +L   G  +S  Y  AFL H  P +P E + ++ NHLLS    AHVS HLGTKDLI         E+ ++ L A+IGALA S     AF F++DFI TQL    L D++       LL  +    + G P PRLL   G N+V A Y VGIY +  LLG+  GE +  A + AA  +L  +F   ENR   DF  + E
Sbjct:   13 LTRSLGCPITVQTHRHIKRWVAPTLRELAHRQKKLGPQKPEPRAGFVEWNERSELFAFGKRLGESFQLPLLQRAFTEKAFARVEEEKRRKLGIEENDLHILDNEDLVEKGRHISKAYVEAFLQHQMPKVPKEGLVEISNHLLSTDTLAHVSSHLGTKDLIHSTEYPPSKESLAESLQAVIGALADSSGVERAFLFVRDFICTQLNQKDLLDIWTPEEPLQLLEKICAERKLGDPEPRLLGDCGKNTVLAAYQVGIYGNRQLLGQGFGEDVQTATETAAIDALQRIFDTHENRRPFDFGLQLE 314
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. TrEMBL
Match: Q7K141 (LD10028p (Fragment) OS=Drosophila melanogaster OX=7227 GN=mRpL44 PE=2 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 6.217e-52
Identity = 116/319 (36.36%), Postives = 170/319 (53.29%), Query Frame = 3 
Query:   27 NIHFKMSFLKSNLPIKISVRYV--------RDITKRLQN---QEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQ-----------DMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDFEDKFE 917
             I  KMSFL+S + +   V+ +        R   KR  +   +E  +R+ ++GP+K  +RS F  WN  SE+FAFGKRL E+F++  L++AF +KS+    E +            M +N++L   G  ++  Y  AFL H  P +P+E +  + ++LLS    AHVS HLGTKDLI         E+++  L A+IGALA S     AF F++DFI TQL    L +V+       LL  +    + G   PRLL   G N+V A Y VGIY++  LLG+  GE +  A + AA  +L ++F   +NR   DF  + E
Sbjct:    9 KIALKMSFLRSAVGLLARVKLLEGSPTVQTRRHIKRWVSPTLRELAHRQKKLGPQKPERRSGFVEWNQRSELFAFGKRLGESFELSELQRAFTEKSFAEREEDRRRQLGIEESDLQMPHNSDLVEKGQQIARAYVEAFLQHQLPKVPNEGLQAIASYLLSTETLAHVSTHLGTKDLIQSTEYPPSAESQAKSLHAVIGALASSSGIERAFIFVRDFICTQLNQKDLLEVWTPQEPIQLLEKICQERKLGEAEPRLLGDCGKNTVLAAYQVGIYANRQLLGKGFGEDVKTATETAALDALQSIFDTRDNRRPFDFAIQLE 327
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Cavefish
Match: mrpl44 (mitochondrial ribosomal protein L44 [Source:NCBI gene;Acc:103033378])

HSP 1 Score: 131.724 bits (330), Expect = 2.223e-35
Identity = 96/273 (35.16%), Postives = 142/273 (52.01%), Query Frame = 3 
Query:  135 RRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINS----------NETQDMLN---NTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVL----FAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDF 902
            RRIE G    + R++  NW+Y +E+ AF  RLKE F  + L+ AF+   Y+ S          N    +LN   NT+LR +G   +  + + +   +FP LP   +  + N+L    +  HV+ +L  ++L    S   +     DVL    FA+IGAL +S     A  F++DF++TQL    L ++++V N   LL   L       P PRL++ +G N+V   Y VG+YSD  L+    GE+LLAAE+ AA  +L  LFG  ENR   DF
Sbjct:   47 RRIE-GEPAPKPRNQQPNWDYHAEVEAFSARLKERFSTELLKTAFVSPCYLRSELERRRLLGLNTEDAVLNIRENTQLREHGQQFTLAFLKDWCKSNFPSLPEAGVTAIVNYLTGSEVMCHVARNLAVEEL----SMAAEFPVPDDVLQGMFFAVIGALEQSSGSERAGIFVRDFLVTQLIGKDLFEIWRVVNPMGLLVEELTCRGVPPPEPRLIQATGTNTVLPLYFVGLYSDQKLIARGPGETLLAAEEEAACVALRKLFGYMENRKPSDF 314
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Nematostella
Match: EDO36101 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJH8])

HSP 1 Score: 98.2117 bits (243), Expect = 6.513e-23
Identity = 73/247 (29.55%), Postives = 113/247 (45.75%), Query Frame = 3 
Query:   93 RDITKRLQNQEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQD---MLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETE-----------SDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDND 791
            RD  +R +N     R+ E    +   + EF   NYE E+FAF  RL   F+ + L +A +       +  +D   +  N++L   G +L+S+Y   +L   +P+LPSE + QL N L +      V+      +LI    D   L+ E           SD   A+IGA+   Q  + A  F++DF++TQL    L  V Q  N   LL  ++L      P  R+ RQSG  +    Y VG++   +
Sbjct:   50 RDTRRRERNLLEKKRKREGQKPRVFDKREFMRENYEKELFAFRNRLSLVFEDERLLRAALTHELCEEDLVKDKEKIEENSKLALLGHNLTSQYITEYLCFKYPNLPSEGVRQLHNFLTNRETLVKVADLTALPELIRTKHDLDSLKKEKHVEVTKEDVISDAFLALIGAIYLDQGSSEANKFVRDFLITQLYTQELNQVLQFENPEALLESMVLSEGHTKPEARITRQSGKATHLPVYVVGLFCGEE 296
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Medaka
Match: mrpl44 (mitochondrial ribosomal protein L44 [Source:NCBI gene;Acc:101155534])

HSP 1 Score: 133.265 bits (334), Expect = 1.186e-35
Identity = 88/264 (33.33%), Postives = 137/264 (51.89%), Query Frame = 3 
Query:  150 GPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSN-ETQDML------------NNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDF 902
            GP   + R +  NW+Y +E+ AF  RL+ENF ++ L+ AFI+  Y+ +  E ++ L            +N +LRN G   +  +   +   +FP LP   +  +  +L S    +HV+ +LG +DL       +  E       A+IGAL +S        F++DF+ TQL    L D++ V +   LL   L   +   P PRL+R +G ++V   Y VG+YSD  LL +  GE+L+ AE+ AAR +L  L+G +ENR   DF
Sbjct:   91 GPPPPKPRWQQPNWDYHAEVQAFSVRLQENFSLELLKTAFINPCYLRAERERREQLGVDSESTALSLKDNVQLRNSGERFAKSFLSDWCRANFPRLPVVGVESIVGNLTSAATVSHVARNLGVEDLSMSADFPVPDEVLRSTFMALIGALKESSGAERTGFFLRDFLSTQLIGKDLFDLWTVVDPMGLLVEELTERKCPLPEPRLIRAAGASTVLPLYFVGLYSDQKLLAQGPGETLVGAEEEAARVALRKLYGFTENRRPFDF 354
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. Planmine SMEST
Match: SMESG000010640.1 (SMESG000010640.1)

HSP 1 Score: 613.994 bits (1582), Expect = 0.000e+0
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 3 
Query:   42 MSFLKSNLPIKISVRYVRDITKRLQNQEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQDMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDFEDKFEIDFNKKPSYSIADYLKCFENK 980
            MSFLKSNLPIKISVRYVRDITKRLQNQEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQDMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDLLGEAAGESLLAAEDMAARHSLLNLFGVSENRSTIDFEDKFEIDFNKKPSYSIADYLKCFENK
Sbjct:    1 MSFLKSNLPIKISVRYVRDITKRLQNQEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQDMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDLLGEAAGESLLAAEDMAARHSLLNLFGVSENRSTIDFEDKFEIDFNKKPSYSIADYLKCFENK 313
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. Planmine SMEST
Match: SMESG000010640.1 (SMESG000010640.1)

HSP 1 Score: 552.362 bits (1422), Expect = 0.000e+0
Identity = 283/288 (98.26%), Postives = 283/288 (98.26%), Query Frame = 3 
Query:  117 NQEFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQDMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDXXXXXXXXXXXXXXDMAARHSLLNLFGVSENRSTIDFEDKFEIDFNKKPSYSIADYLKCFENK 980
            N  F   RIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQDMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDLLGEAAGESLLAAEDMAARHSLLNLFGVSENRSTIDFEDKFEIDFNKKPSYSIADYLKCFENK
Sbjct:   13 NMTFEKTRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQDMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSVFACYHVGIYSDNDLLGEAAGESLLAAEDMAARHSLLNLFGVSENRSTIDFEDKFEIDFNKKPSYSIADYLKCFENK 300
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. Planmine SMEST
Match: SMESG000010661.1 (SMESG000010661.1)

HSP 1 Score: 406.371 bits (1043), Expect = 1.524e-143
Identity = 195/200 (97.50%), Postives = 197/200 (98.50%), Query Frame = 3 
Query:  123 EFYYRRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNETQDMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPR 722
            E   +RIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFD+KYLRQAFIDKSYINSNETQDMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPR
Sbjct:   45 EILIKRIEIGPEKARQRSEFSNWNYESEIFAFGKRLKENFDMKYLRQAFIDKSYINSNETQDMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPR 244
[Close]
BLAST of 39S ribosomal protein L44, mitochondrial vs. Planmine SMEST
Match: SMESG000010661.1 (SMESG000010661.1)

HSP 1 Score: 286.574 bits (732), Expect = 7.036e-98
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 3 
Query:  309 MLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPR 722
            MLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPR
Sbjct:    1 MLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLTAHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIKDFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPR 138
The following BLAST results are available for this feature:
BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MRPL442.523e-4033.00mitochondrial ribosomal protein L44 [Source:HGNC S... [more]
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BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl-441.650e-2328.77Mitochondrial Ribosomal Protein, Large [Source:Re... [more]
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BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mRpL443.939e-5436.31gene:FBgn0037330 transcript:FBtr0078768[more]
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BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mrpl442.044e-4034.09mitochondrial ribosomal protein L44 [Source:ZFIN;A... [more]
mrpl446.162e-1842.98mitochondrial ribosomal protein L44 [Source:ZFIN;A... [more]
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BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl442.498e-4235.15mitochondrial ribosomal protein L44 [Source:NCBI g... [more]
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BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Mrpl443.323e-4234.92mitochondrial ribosomal protein L44 [Source:MGI Sy... [more]
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BLAST of 39S ribosomal protein L44, mitochondrial vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9CY73|RM44_MOUSE2.324e-4134.9239S ribosomal protein L44, mitochondrial OS=Mus mu... [more]
sp|Q2KIS2|RM44_BOVIN1.150e-3933.4539S ribosomal protein L44, mitochondrial OS=Bos ta... [more]
sp|Q9H9J2|RM44_HUMAN1.212e-3933.0039S ribosomal protein L44, mitochondrial OS=Homo s... [more]
sp|Q5REY8|RM44_PONAB2.399e-3933.9339S ribosomal protein L44, mitochondrial OS=Pongo ... [more]
sp|P34384|RM44_CAEEL2.098e-2228.77Ribosomal protein L44, mitochondrial OS=Caenorhabd... [more]
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BLAST of 39S ribosomal protein L44, mitochondrial vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2C9KAB18.348e-5738.59RNase III domain-containing protein OS=Biomphalari... [more]
A0A0R3SCF89.644e-5439.09RNase III domain-containing protein OS=Hymenolepis... [more]
B4KD972.310e-5240.79RNase III domain-containing protein OS=Drosophila ... [more]
B3LZH83.739e-5237.75RNase III domain-containing protein OS=Drosophila ... [more]
Q7K1416.217e-5236.36LD10028p (Fragment) OS=Drosophila melanogaster OX=... [more]
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BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl442.223e-3535.16mitochondrial ribosomal protein L44 [Source:NCBI g... [more]
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BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO361016.513e-2329.55Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of 39S ribosomal protein L44, mitochondrial vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl441.186e-3533.33mitochondrial ribosomal protein L44 [Source:NCBI g... [more]
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BLAST of 39S ribosomal protein L44, mitochondrial vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000010640.10.000e+0100.00SMESG000010640.1[more]
SMESG000010640.10.000e+098.26SMESG000010640.1[more]
SMESG000010661.11.524e-14397.50SMESG000010661.1[more]
SMESG000010661.17.036e-98100.00SMESG000010661.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30001033 ID=SMED30001033|Name=39S ribosomal protein L44, mitochondrial|organism=Schmidtea mediterranea sexual|type=transcript|length=1006bp
TTTACAATAGACAATACAACTGTTTAAATATTCATTTTAAAATGAGCTTT
TTAAAGTCAAATTTACCGATAAAAATTTCTGTTCGTTATGTGCGGGATAT
TACTAAGAGATTGCAAAATCAAGAATTTTATTATAGAAGAATAGAAATTG
GTCCTGAAAAAGCAAGACAGAGATCAGAATTTTCTAATTGGAATTATGAA
TCCGAAATTTTTGCATTCGGTAAAAGATTGAAAGAAAATTTTGATATAAA
ATATCTTAGGCAGGCATTTATTGACAAATCTTATATTAATAGTAATGAAA
CTCAAGATATGTTAAATAATACTGAATTGAGGAATTATGGTTTTAGTCTG
TCAAGTAAATACACTCGAGCTTTCCTAATTCATAGTTTTCCTGATTTACC
ATCTGAATGGATAAATCAGTTATGTAATCATCTGCTATCTATACATTTAA
CAGCACATGTTAGTGGCCATCTCGGCACAAAGGATCTGATTTTTTATAAT
TCTGATACACTTGACTTGGAAACTGAGTCAGATGTTTTATTTGCTATAAT
AGGAGCATTGGCTAAAAGTCAGGATGAAAATGTAGCATTTACATTTATCA
AAGATTTTATTCTAACCCAATTAACTGTATTTCATTTGTGCGATGTTTTT
CAAGTCAATAATGCTTTTAATTTGTTATCAGGAGTGTTATTATATCACGA
GAAAGGAGCTCCTGTGCCTCGGCTGCTGCGTCAATCTGGAATGAATTCAG
TATTTGCCTGCTATCATGTTGGTATTTACAGTGATAATGATTTGCTAGGG
GAAGCTGCTGGTGAATCGCTTTTGGCAGCAGAAGATATGGCTGCTAGGCA
TTCGCTGCTTAATCTGTTTGGAGTATCTGAGAATAGATCAACAATCGACT
TTGAAGATAAATTTGAAATTGACTTTAATAAAAAGCCGTCTTATAGTATT
GCTGATTATTTGAAATGTTTTGAAAATAAATAATATTTTTCGTTTTTAAA
AAAAAA
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protein sequence of SMED30001033-orf-1

>SMED30001033-orf-1 ID=SMED30001033-orf-1|Name=SMED30001033-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=327bp
YNRQYNCLNIHFKMSFLKSNLPIKISVRYVRDITKRLQNQEFYYRRIEIG
PEKARQRSEFSNWNYESEIFAFGKRLKENFDIKYLRQAFIDKSYINSNET
QDMLNNTELRNYGFSLSSKYTRAFLIHSFPDLPSEWINQLCNHLLSIHLT
AHVSGHLGTKDLIFYNSDTLDLETESDVLFAIIGALAKSQDENVAFTFIK
DFILTQLTVFHLCDVFQVNNAFNLLSGVLLYHEKGAPVPRLLRQSGMNSV
FACYHVGIYSDNDLLGEAAGESLLAAEDMAARHSLLNLFGVSENRSTIDF
EDKFEIDFNKKPSYSIADYLKCFENK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR036389RNase_III_sf
IPR000999RNase_III_dom
IPR018222Nuclear_transport_factor_2_euk
Vocabulary: molecular function
TermDefinition
GO:0004525ribonuclease III activity
GO:0003723RNA binding
GO:0004518nuclease activity
GO:0004519endonuclease activity
GO:0016787hydrolase activity
Vocabulary: biological process
TermDefinition
GO:0006396RNA processing
GO:0070125mitochondrial translational elongation
GO:0090305nucleic acid phosphodiester bond hydrolysis
GO:0090501RNA phosphodiester bond hydrolysis
GO:0090502RNA phosphodiester bond hydrolysis, endonucleolytic
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005739mitochondrion
GO:0005762mitochondrial large ribosomal subunit
GO:0005840ribosome
GO:0005886plasma membrane
GO:0030529intracellular ribonucleoprotein complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000999Ribonuclease III domainSMARTSM00535riboneu5coord: 83..212
e-value: 0.0078
score: 12.0
IPR000999Ribonuclease III domainPROSITEPS50142RNASE_3_2coord: 72..191
score: 9.102
IPR036389Ribonuclease III, endonuclease domain superfamilyGENE3DG3DSA:1.10.1520.10coord: 35..162
e-value: 9.3E-13
score: 50.2
IPR036389Ribonuclease III, endonuclease domain superfamilySUPERFAMILYSSF69065RNase III domain-likecoord: 61..207
NoneNo IPR availableGENE3DG3DSA:3.30.160.20coord: 217..306
e-value: 1.8E-14
score: 55.4
NoneNo IPR availablePANTHERPTHR11207:SF539S RIBOSOMAL PROTEIN L44, MITOCHONDRIALcoord: 30..302
NoneNo IPR availablePANTHERPTHR11207RIBONUCLEASE IIIcoord: 30..302
IPR018222Nuclear transport factor 2, eukaryotePROSITEPS50177NTF2_DOMAINcoord: 85..231
score: 8.813